Pub Date : 2022-01-01DOI: 10.1177/1721727X221139485
Jian Xin, Y. Shang
The breast cancer is most frequently diagnosed cancer in the women worldwide. Our study investigated the anticancer effect of alternariol, a secondary metabolite originated from endophytic fungi, against DMBA induced breast carcinoma on Wistar rats. The toxicity study investigated the LD50 and the subsequent doses of alternariol for the carcinogenic study. The breast cancer was developed in rats via induction of DMBA (5 mg/kg, i.v.) and the carcinogenic study was continued for 24 weeks. The induction of breast cancer and the chemotherapeutic effect of alternariol were assessed through histopathological analysis of rat mammary tissue, followed by immunohistochemical analysis, cell proliferation assay and apoptosis assay by TUNEL method. The result showed that alternariol therapy decreased the hyperplastic lesions of mammary tissue and restored the normal histopathological characteristics of breast tissue. Furthermore, alternariol treatment downregulated the expression of carcinogenic markers such as PI3K and Akt increased the expression of apoptotic markers including p53, caspase-3 and Bax. Alternariol therapy also decreased the cellular proliferation and enhanced the apoptotic events. In conclusion, the breast cancer progression was significantly reduced via induction of apoptotic events and inhibition of cell propagation which allowed constructing of suitable mechanism for alternariol mediated chemotherapeutic approach.
{"title":"Alternariol alleviates breast carcinoma by inhibiting cellular proliferation correlated with increased apoptotic events in rats","authors":"Jian Xin, Y. Shang","doi":"10.1177/1721727X221139485","DOIUrl":"https://doi.org/10.1177/1721727X221139485","url":null,"abstract":"The breast cancer is most frequently diagnosed cancer in the women worldwide. Our study investigated the anticancer effect of alternariol, a secondary metabolite originated from endophytic fungi, against DMBA induced breast carcinoma on Wistar rats. The toxicity study investigated the LD50 and the subsequent doses of alternariol for the carcinogenic study. The breast cancer was developed in rats via induction of DMBA (5 mg/kg, i.v.) and the carcinogenic study was continued for 24 weeks. The induction of breast cancer and the chemotherapeutic effect of alternariol were assessed through histopathological analysis of rat mammary tissue, followed by immunohistochemical analysis, cell proliferation assay and apoptosis assay by TUNEL method. The result showed that alternariol therapy decreased the hyperplastic lesions of mammary tissue and restored the normal histopathological characteristics of breast tissue. Furthermore, alternariol treatment downregulated the expression of carcinogenic markers such as PI3K and Akt increased the expression of apoptotic markers including p53, caspase-3 and Bax. Alternariol therapy also decreased the cellular proliferation and enhanced the apoptotic events. In conclusion, the breast cancer progression was significantly reduced via induction of apoptotic events and inhibition of cell propagation which allowed constructing of suitable mechanism for alternariol mediated chemotherapeutic approach.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49465395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221128266
Xue Du, Yu Xiao, Chunbao Chen, Lu Yang, Yu Cui, Tingting Wu, B. Tan
Objective To establish an effective cuproptosis-related long non-coding ribonucleic acid (lncRNA) (CRL) prognostic risk score (RS) model (CRLPRSM) for the prediction of the outcomes of patients with gastric cancer (GC). Introduction Cuproptosis is an up-to-date mode of cell death, and its action mechanism is different from all other known mechanisms that regulate cell death. LncRNAs are RNA species that are over 200 nt long and do not encode proteins. They have prominent actions in tumor onset and development, and their involvement in a variety of intracellular regulatory processes is vital for cell proliferation and differentiation, so they may serve as prognostic biomarkers in tumor patients. Methods We retrieved cuproptosis-associated clinical and lncRNA expression data of GC cases from The Cancer Genome Atlas Stomach Adenocarcinoma (TCGA-STAD). Then a CRLPRSM was built based on univariate and multivariate Cox regression (UCR and MCR) analyses. As per the RS, the patients fell into high- and low-risk group. Later, the predictive efficacy of the CRLPRSM was confirmed with the aid of Kaplan-Meier (KM) analysis and receiver operating characteristic (ROC) curve analysis. Next, combining independent prognostic factors in clinical characteristics, we plotted a prognosis-related nomogram to predict one-year, three-year and five-year overall survival (OS) in GC patients. Finally, we implemented Gene Ontology enrichment analysis (GOEA) and Kyoto Encyclopedia of Genes and Genomes enrichment analysis (KEGGEA) for clarifying the possible biological actions and molecular mechanisms. Results The constructed CRLPRSM consisted of 3 CRLs, namely, AC092574.1, MAGI2-AS3, AC090204.1. It was found that the hazard ratio (HR) was 1.911 (1.337–2.731) (p < 0.001) in UCR analysis and 1.852 (1.286–1.668) (p < 0.001) in MCR analysis, and the AUC of the CRLPRSM was 0.649. Moreover, the KM analysis showed a pronounced intergroup difference in survival, and the nomogram illustrated some clinical benefits of CRLPRSM. Furthermore, GO terms and KEGG pathways were unveiled to be significantly enriched. Conclusion The constructed CRLPRSM has a significant predicted value for GC patient prognosis, and CRLs may become novel hallmarks for clinical treatment of GC.
{"title":"A novel cuproptosis-related lncRNA prognostic risk score model for gastric cancer based on bioinformatics","authors":"Xue Du, Yu Xiao, Chunbao Chen, Lu Yang, Yu Cui, Tingting Wu, B. Tan","doi":"10.1177/1721727X221128266","DOIUrl":"https://doi.org/10.1177/1721727X221128266","url":null,"abstract":"Objective To establish an effective cuproptosis-related long non-coding ribonucleic acid (lncRNA) (CRL) prognostic risk score (RS) model (CRLPRSM) for the prediction of the outcomes of patients with gastric cancer (GC). Introduction Cuproptosis is an up-to-date mode of cell death, and its action mechanism is different from all other known mechanisms that regulate cell death. LncRNAs are RNA species that are over 200 nt long and do not encode proteins. They have prominent actions in tumor onset and development, and their involvement in a variety of intracellular regulatory processes is vital for cell proliferation and differentiation, so they may serve as prognostic biomarkers in tumor patients. Methods We retrieved cuproptosis-associated clinical and lncRNA expression data of GC cases from The Cancer Genome Atlas Stomach Adenocarcinoma (TCGA-STAD). Then a CRLPRSM was built based on univariate and multivariate Cox regression (UCR and MCR) analyses. As per the RS, the patients fell into high- and low-risk group. Later, the predictive efficacy of the CRLPRSM was confirmed with the aid of Kaplan-Meier (KM) analysis and receiver operating characteristic (ROC) curve analysis. Next, combining independent prognostic factors in clinical characteristics, we plotted a prognosis-related nomogram to predict one-year, three-year and five-year overall survival (OS) in GC patients. Finally, we implemented Gene Ontology enrichment analysis (GOEA) and Kyoto Encyclopedia of Genes and Genomes enrichment analysis (KEGGEA) for clarifying the possible biological actions and molecular mechanisms. Results The constructed CRLPRSM consisted of 3 CRLs, namely, AC092574.1, MAGI2-AS3, AC090204.1. It was found that the hazard ratio (HR) was 1.911 (1.337–2.731) (p < 0.001) in UCR analysis and 1.852 (1.286–1.668) (p < 0.001) in MCR analysis, and the AUC of the CRLPRSM was 0.649. Moreover, the KM analysis showed a pronounced intergroup difference in survival, and the nomogram illustrated some clinical benefits of CRLPRSM. Furthermore, GO terms and KEGG pathways were unveiled to be significantly enriched. Conclusion The constructed CRLPRSM has a significant predicted value for GC patient prognosis, and CRLs may become novel hallmarks for clinical treatment of GC.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49566365","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221141789
Yuandong Su, Wei Cui
Objectives: This study aimed to evaluate the correlation of serum biochemical indexes with disease activity and pruritus in patients with chronic spontaneous urticaria (CSU). Methods: Fifty CSU patients were enrolled and 50 health screen examinees were recruited as the control group. Peripheral blood samples were collected, and thyroid colour Doppler ultrasounds were recorded. Body mass index (BMI) was calculated for both groups. The urticaria activity score over 7 days (UAS-7) was used to evaluate the disease activity and degree of pruritus. Results: There was no significant difference in BMI between CSU patients and control (p > .05). The serum WBC, NEU, CRP (all p = .000) and ALT levels (p = .013) in the CSU patients were significantly higher than those in the healthy control group, and the UAS-7 score was positively correlated with WBC and NEU in the CSU patient group. The TG level of the CSU group was higher than healthy controls, albeit no statistical significance (p = .522). The levels of T3 and TSH were significantly lower in the CSU group (p = .000). The incidence rate of thyroid nodules in the CSU patients was significantly higher (p = .045), and four CSU patients showed papillary carcinoma by pathology examination. The average UAS-7 score of patients with complications was significantly higher than that of patients without complications. Conclusion: Patients experiencing CSU generally exhibit abnormal serum biochemical indexes and thyroid function. Their incidence rates of thyroid nodule and thyroid papillary carcinoma are significantly higher than that of healthy subjects, which should be considered in clinical practice.
{"title":"Association of serum biochemical indexes and thyroid nodule with disease activity in patients with chronic spontaneous urticaria","authors":"Yuandong Su, Wei Cui","doi":"10.1177/1721727X221141789","DOIUrl":"https://doi.org/10.1177/1721727X221141789","url":null,"abstract":"Objectives: This study aimed to evaluate the correlation of serum biochemical indexes with disease activity and pruritus in patients with chronic spontaneous urticaria (CSU). Methods: Fifty CSU patients were enrolled and 50 health screen examinees were recruited as the control group. Peripheral blood samples were collected, and thyroid colour Doppler ultrasounds were recorded. Body mass index (BMI) was calculated for both groups. The urticaria activity score over 7 days (UAS-7) was used to evaluate the disease activity and degree of pruritus. Results: There was no significant difference in BMI between CSU patients and control (p > .05). The serum WBC, NEU, CRP (all p = .000) and ALT levels (p = .013) in the CSU patients were significantly higher than those in the healthy control group, and the UAS-7 score was positively correlated with WBC and NEU in the CSU patient group. The TG level of the CSU group was higher than healthy controls, albeit no statistical significance (p = .522). The levels of T3 and TSH were significantly lower in the CSU group (p = .000). The incidence rate of thyroid nodules in the CSU patients was significantly higher (p = .045), and four CSU patients showed papillary carcinoma by pathology examination. The average UAS-7 score of patients with complications was significantly higher than that of patients without complications. Conclusion: Patients experiencing CSU generally exhibit abnormal serum biochemical indexes and thyroid function. Their incidence rates of thyroid nodule and thyroid papillary carcinoma are significantly higher than that of healthy subjects, which should be considered in clinical practice.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43085428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221105131
Zhongsheng Zhu, Jinyu Li, R. Tong, Xiaorong Zhang, Bo Yu
Introduction Astaxanthin (ASX) is carotenoid with the highest antioxidant activity in various cell types and reverse atherosclerosis. However, the roles and detailed mechanisms of ASX in atherosclerosis associated endothelial injury remains unclear. Methods In vitro atherosclerosis model was established in HUVECs by incubation with oxidized low-density lipoprotein (ox-LDL). Cell viability and oxidative stress were measured. The mRNA and protein expressions of lectin-like ox-LDL receptor (LOX-1) and other related genes were determined. Results ox-LDL reduced cell viability of HUVECs, and induced oxidative stress, as evidenced by elevated cellular malondialdehyde (MDA) and decreased superoxide dismutase (SOD). Pretreatment with ASX (50, 100, 200, and 400 μM) markedly reversed the reduction in cell viability and an increase in migration of HUVECs induced by ox-LDL (50 μg/mL). ASX attenuated the increase in the endothelial-to-mesenchymal transition (EndMT) process, as evidenced by increased CD31 and decreased α-SMA and vimentin proteins by ASX treatment in HUVECs. Furthermore, ASX attenuated the increase in MDA and decrease in SOD induced by ox-LDL, increased supernatant NO production, attenuated the increase in iNOS and decrease in eNOS in HUVECs with ox-LDL. ASX enhanced mRNA and protein expressions of the lectin-like ox-LDL receptor (LOX-1), which was dependent on ASX’s antioxidant activity. The inhibitory effect of ASX on EndMT could be abolished by overexpression of LOX-1 in HUVECs induced by ox-LDL. Conclusions Our data speculate that ASX prevents ox-LDL-induced endothelial cell injury and EndMT by inducing antioxidant property (SOD and NO) and decreasing LOX-1 expression.
{"title":"Astaxanthin suppresses End MT by LOX-1 pathway in ox-LDL-induced HUVECs","authors":"Zhongsheng Zhu, Jinyu Li, R. Tong, Xiaorong Zhang, Bo Yu","doi":"10.1177/1721727X221105131","DOIUrl":"https://doi.org/10.1177/1721727X221105131","url":null,"abstract":"Introduction Astaxanthin (ASX) is carotenoid with the highest antioxidant activity in various cell types and reverse atherosclerosis. However, the roles and detailed mechanisms of ASX in atherosclerosis associated endothelial injury remains unclear. Methods In vitro atherosclerosis model was established in HUVECs by incubation with oxidized low-density lipoprotein (ox-LDL). Cell viability and oxidative stress were measured. The mRNA and protein expressions of lectin-like ox-LDL receptor (LOX-1) and other related genes were determined. Results ox-LDL reduced cell viability of HUVECs, and induced oxidative stress, as evidenced by elevated cellular malondialdehyde (MDA) and decreased superoxide dismutase (SOD). Pretreatment with ASX (50, 100, 200, and 400 μM) markedly reversed the reduction in cell viability and an increase in migration of HUVECs induced by ox-LDL (50 μg/mL). ASX attenuated the increase in the endothelial-to-mesenchymal transition (EndMT) process, as evidenced by increased CD31 and decreased α-SMA and vimentin proteins by ASX treatment in HUVECs. Furthermore, ASX attenuated the increase in MDA and decrease in SOD induced by ox-LDL, increased supernatant NO production, attenuated the increase in iNOS and decrease in eNOS in HUVECs with ox-LDL. ASX enhanced mRNA and protein expressions of the lectin-like ox-LDL receptor (LOX-1), which was dependent on ASX’s antioxidant activity. The inhibitory effect of ASX on EndMT could be abolished by overexpression of LOX-1 in HUVECs induced by ox-LDL. Conclusions Our data speculate that ASX prevents ox-LDL-induced endothelial cell injury and EndMT by inducing antioxidant property (SOD and NO) and decreasing LOX-1 expression.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45273796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221144391
U. Saleem, N. Aslam, R. Siddique, S. Iqbal, M. Manan
Hepatitis C is a dangerous liver disease transmitted by Hepatitis C virus (HCV). HCV constitutes an important health issue in Pakistan. In Pakistani setting HCV is found frequently and is recognized as an alarming health problem. In this cross sectional study we reviewed published data regarding the seroprevalence of hepatitis C in general community, blood donors and pregnant females and risk factors linked with its occurrence in Pakistan. Data retrieved from163 studies published from 2001 to 2022 was utilized and weighted mean was calculated. Data of 1,875,232 individuals was collected and arranged into three groups, depending upon the population type such as (1) general population, (2) pregnant women, (3) blood donors. General population (765,426) and blood donors (973,260) formed the most of population. Mean Hepatitis C virus prevalence in general public and blood donors was 16.47% and 8.2% respectively. In pregnant females (136,546) the mean frequency was 9.3%. This study exhibits that the frequency of Hepatitis C in general population, pregnant females and blood donors groups was 11.32%. The data suggested that risks factors for transmitting HCV infection in Pakistan include unsterilized needle use, blood transfusions, shaving by barbers, lack of trained staff, needle stick injuries, injection drug users, household contacts/spousal transmission, unsterilized dental and surgical Instruments, improper disposal of hospital waste, poor infra-structure and others. The frequency of HCV infection is distressing in Pakistan. Health education and awareness programs are needed for decreasing Hepatitis C infection in Pakistan. The data necessitate the implementation of preventive and remedial approaches to decrease the disease load and mortality in Pakistan.
{"title":"Hepatitis C virus: Its prevalence, risk factors and genotype distribution in Pakistan","authors":"U. Saleem, N. Aslam, R. Siddique, S. Iqbal, M. Manan","doi":"10.1177/1721727X221144391","DOIUrl":"https://doi.org/10.1177/1721727X221144391","url":null,"abstract":"Hepatitis C is a dangerous liver disease transmitted by Hepatitis C virus (HCV). HCV constitutes an important health issue in Pakistan. In Pakistani setting HCV is found frequently and is recognized as an alarming health problem. In this cross sectional study we reviewed published data regarding the seroprevalence of hepatitis C in general community, blood donors and pregnant females and risk factors linked with its occurrence in Pakistan. Data retrieved from163 studies published from 2001 to 2022 was utilized and weighted mean was calculated. Data of 1,875,232 individuals was collected and arranged into three groups, depending upon the population type such as (1) general population, (2) pregnant women, (3) blood donors. General population (765,426) and blood donors (973,260) formed the most of population. Mean Hepatitis C virus prevalence in general public and blood donors was 16.47% and 8.2% respectively. In pregnant females (136,546) the mean frequency was 9.3%. This study exhibits that the frequency of Hepatitis C in general population, pregnant females and blood donors groups was 11.32%. The data suggested that risks factors for transmitting HCV infection in Pakistan include unsterilized needle use, blood transfusions, shaving by barbers, lack of trained staff, needle stick injuries, injection drug users, household contacts/spousal transmission, unsterilized dental and surgical Instruments, improper disposal of hospital waste, poor infra-structure and others. The frequency of HCV infection is distressing in Pakistan. Health education and awareness programs are needed for decreasing Hepatitis C infection in Pakistan. The data necessitate the implementation of preventive and remedial approaches to decrease the disease load and mortality in Pakistan.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44164666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221098970
Hamid Chegni, H. Babaii, Z. Hassan, M. Pourshaban
In 2020, a deadly pandemic caused by the SARS-COV-2 virus spread worldwide and killed many people. In some viral infections, in addition to the pathogenic role of the virus, impaired immune function leads to inflammation and further damage in internal tissues. For example, coronavirus in some patients prevents the stimulation of the acquired immune system. Therefore, innate immunity is over-stimulated to compensate, followed by the overproduction of inflammatory cytokines and cytokine storm. Various underlying factors such as age, gender, blood pressure, diabetes, and obesity affect cytokine storm. It seems that cytokine storm is one of the leading causes of death among COVID-19 patients, and providing that this storm is detected and controlled in time, it can reduce the mortality of COVID-19 patients. This article aims to investigate the immune system response to COVID-19, various factors associated with cytokine storm, and its treatment. In the current situation, in parallel with the progress made in the field of vaccination, it is necessary to carefully examine the various dimensions of the immune system in response to the COVID-19 virus to seek a suitable treatment strategy to save the lives of patients in intensive care units
{"title":"Immune response and cytokine storm in SARS-CoV-2 infection: Risk factors, ways of control and treatment","authors":"Hamid Chegni, H. Babaii, Z. Hassan, M. Pourshaban","doi":"10.1177/1721727X221098970","DOIUrl":"https://doi.org/10.1177/1721727X221098970","url":null,"abstract":"In 2020, a deadly pandemic caused by the SARS-COV-2 virus spread worldwide and killed many people. In some viral infections, in addition to the pathogenic role of the virus, impaired immune function leads to inflammation and further damage in internal tissues. For example, coronavirus in some patients prevents the stimulation of the acquired immune system. Therefore, innate immunity is over-stimulated to compensate, followed by the overproduction of inflammatory cytokines and cytokine storm. Various underlying factors such as age, gender, blood pressure, diabetes, and obesity affect cytokine storm. It seems that cytokine storm is one of the leading causes of death among COVID-19 patients, and providing that this storm is detected and controlled in time, it can reduce the mortality of COVID-19 patients. This article aims to investigate the immune system response to COVID-19, various factors associated with cytokine storm, and its treatment. In the current situation, in parallel with the progress made in the field of vaccination, it is necessary to carefully examine the various dimensions of the immune system in response to the COVID-19 virus to seek a suitable treatment strategy to save the lives of patients in intensive care units","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44426615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221092909
Chaoyang Hua, Jia Li, Yanfang Yang, Zhan-Yun Liu
Background: Coronavirus disease 2019 (COVID-19) became pandemic in 2020 and recently, mutated coronaviruses have emerged in many countries. The aim of this study was to identify the clinical characteristics and risk factors for critical illness in hospitalized COVID-19 patients in Zhengzhou for clinical prevention and management. Materials and methods: A total of 70 patients hospitalized with COVID-19 were enrolled between 21 January and 29 February 2020, in Zhengzhou, China. Clinical characteristics, hematological findings, neutrophil lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and inflammatory index on admission were obtained from medical records, COVID-19 patients with different outcomes were compared. Results: The median age was 55 years. Forty-three (61.0%) patients were classified as having severe or critical cases. Eighteen (25.7%) patients died in hospital and the remaining 52 were discharged. Patients who died tend to be old with expectoration and chronic obstructive pulmonary disease. Compared to survivor, non-survivor had significantly higher numbers of leucocytes and neutrophils, NLR, aspartate aminotransferase (AST), γ-glutamyl transpeptidase, total bilirubin, direct bilirubin, lactate dehydrogenase (LDH), prothrombin time, D-dimer, C-reactive protein, and decreased platelets, lymphocytes, uric acid, and albumin (ALB). Logistic regression analysis identified leucocytes, platelets, PLR, NLR, AST, and ALB as independent predictive factors for poor outcomes. The area under curve of the combination of leucocytes, PLR, NLR, and AST was 0.87, with a sensitivity of 0.83 and specificity of 0.81. Conclusion: Our results identified risk factors among COVID-19 patients for in-hospital mortality. Leucocytes, PLR, NLR, and AST could have important reference value for predicting prognosis, especially in low-resource countries.
{"title":"Hematological features and risk factors of hospitalized COVID-19 patients: A retrospective analysis","authors":"Chaoyang Hua, Jia Li, Yanfang Yang, Zhan-Yun Liu","doi":"10.1177/1721727X221092909","DOIUrl":"https://doi.org/10.1177/1721727X221092909","url":null,"abstract":"Background: Coronavirus disease 2019 (COVID-19) became pandemic in 2020 and recently, mutated coronaviruses have emerged in many countries. The aim of this study was to identify the clinical characteristics and risk factors for critical illness in hospitalized COVID-19 patients in Zhengzhou for clinical prevention and management. Materials and methods: A total of 70 patients hospitalized with COVID-19 were enrolled between 21 January and 29 February 2020, in Zhengzhou, China. Clinical characteristics, hematological findings, neutrophil lymphocyte ratio (NLR), platelet lymphocyte ratio (PLR), and inflammatory index on admission were obtained from medical records, COVID-19 patients with different outcomes were compared. Results: The median age was 55 years. Forty-three (61.0%) patients were classified as having severe or critical cases. Eighteen (25.7%) patients died in hospital and the remaining 52 were discharged. Patients who died tend to be old with expectoration and chronic obstructive pulmonary disease. Compared to survivor, non-survivor had significantly higher numbers of leucocytes and neutrophils, NLR, aspartate aminotransferase (AST), γ-glutamyl transpeptidase, total bilirubin, direct bilirubin, lactate dehydrogenase (LDH), prothrombin time, D-dimer, C-reactive protein, and decreased platelets, lymphocytes, uric acid, and albumin (ALB). Logistic regression analysis identified leucocytes, platelets, PLR, NLR, AST, and ALB as independent predictive factors for poor outcomes. The area under curve of the combination of leucocytes, PLR, NLR, and AST was 0.87, with a sensitivity of 0.83 and specificity of 0.81. Conclusion: Our results identified risk factors among COVID-19 patients for in-hospital mortality. Leucocytes, PLR, NLR, and AST could have important reference value for predicting prognosis, especially in low-resource countries.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44508329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221131792
Laxmi Prasad Uprety, Y. Park, Y. Jang
Introduction Lupus nephritis (LN), a severe manifestation of systemic lupus erythematosus (SLE), is associated with high fatality rate in patients. The pathogenesis of lupus nephritis is complex and has not been fully elucidated. Kidney inflammation, renal cell damage, and accumulation of immune complexes in the glomerular basement membrane often occur in patients with lupus nephritis. Spermatid nuclear transition protein 1 (TNP1) might be a potentially interesting autoantigen in exploring the pathogenesis and therapy of lupus nephritis. Objective This study aimed to explore the effect of TNP1 and its complexes with anti-double-stranded DNA antibodies on the levels of interleukin-6 (IL-6) and interferon-α (IFN-α) in vitro. Methods We studied the effect of the synthetic peptide of the autoantigen on the pathogenic characteristics of the G2-6 and G5-8 antibodies in mouse macrophages, using enzyme-linked immunosorbent assay, quantitative RT-PCR, western blotting, and flow cytometry. Results The antibodies exhibited cross-reactivity to spermatid TNP1 in direct-binding and competitive enzyme-linked immunosorbent assay. Results of quantitative RT-PCR and western blotting revealed that the antibodies alone enhanced the levels of IL-6 and IFN-α transcripts and proteins, respectively. Flow cytometry revealed that treatment with the autoantigen enhanced the cell-penetrating activities of G2-6 and G5-8 and remarkably increased the cytokine levels. Conclusion TNP1 enhanced the cell-penetrating activities of anti-dsDNA auto-Abs, G2-6 and G5-8, and remarkably increased the levels of IL-6 and IFN-α in macrophages, suggesting that TNP1 and cell-penetrating pathogenic anti-dsDNA auto-Abs is potential targets for future therapeutic approaches to treat LN/SLE.
{"title":"Autoantigen spermatid nuclear transition protein 1 enhances pro-inflammatory cytokine production stimulated by anti-DNA autoantibodies in macrophages","authors":"Laxmi Prasad Uprety, Y. Park, Y. Jang","doi":"10.1177/1721727X221131792","DOIUrl":"https://doi.org/10.1177/1721727X221131792","url":null,"abstract":"Introduction Lupus nephritis (LN), a severe manifestation of systemic lupus erythematosus (SLE), is associated with high fatality rate in patients. The pathogenesis of lupus nephritis is complex and has not been fully elucidated. Kidney inflammation, renal cell damage, and accumulation of immune complexes in the glomerular basement membrane often occur in patients with lupus nephritis. Spermatid nuclear transition protein 1 (TNP1) might be a potentially interesting autoantigen in exploring the pathogenesis and therapy of lupus nephritis. Objective This study aimed to explore the effect of TNP1 and its complexes with anti-double-stranded DNA antibodies on the levels of interleukin-6 (IL-6) and interferon-α (IFN-α) in vitro. Methods We studied the effect of the synthetic peptide of the autoantigen on the pathogenic characteristics of the G2-6 and G5-8 antibodies in mouse macrophages, using enzyme-linked immunosorbent assay, quantitative RT-PCR, western blotting, and flow cytometry. Results The antibodies exhibited cross-reactivity to spermatid TNP1 in direct-binding and competitive enzyme-linked immunosorbent assay. Results of quantitative RT-PCR and western blotting revealed that the antibodies alone enhanced the levels of IL-6 and IFN-α transcripts and proteins, respectively. Flow cytometry revealed that treatment with the autoantigen enhanced the cell-penetrating activities of G2-6 and G5-8 and remarkably increased the cytokine levels. Conclusion TNP1 enhanced the cell-penetrating activities of anti-dsDNA auto-Abs, G2-6 and G5-8, and remarkably increased the levels of IL-6 and IFN-α in macrophages, suggesting that TNP1 and cell-penetrating pathogenic anti-dsDNA auto-Abs is potential targets for future therapeutic approaches to treat LN/SLE.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42786902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221104389
Xin Li, Zhiqiang Wang
Oral lichen planus is a chronic non-infectious mucosal inflammatory disease caused by an imbalance between reactive oxygen species homeostasis and antioxidant defense systems. Notably, excessive oxidative stress products result in related autoimmune reactions. Further, it activates signaling pathways related to the development of oral lichen planus, as evidenced by the detection of damage to deoxyribonucleic acid, protein, and lipid. Thus, the mechanisms of reactive oxygen species-mediated oxidative stress in the pathogenesis of oral lichen planus are numerous and complex. In this review, we first introduce oxidative stress and oxidative products. Then, we summarize the role and possible mechanisms of reactive oxygen species-mediated oxidative stress in the pathogenesis of oral lichen planus and present a clinical correlation between oxidative stress and oral lichen planus. Finally, we discuss the current challenges and future perspectives.
{"title":"Mechanisms of reactive oxygen species in oral lichen planus: A literature review","authors":"Xin Li, Zhiqiang Wang","doi":"10.1177/1721727X221104389","DOIUrl":"https://doi.org/10.1177/1721727X221104389","url":null,"abstract":"Oral lichen planus is a chronic non-infectious mucosal inflammatory disease caused by an imbalance between reactive oxygen species homeostasis and antioxidant defense systems. Notably, excessive oxidative stress products result in related autoimmune reactions. Further, it activates signaling pathways related to the development of oral lichen planus, as evidenced by the detection of damage to deoxyribonucleic acid, protein, and lipid. Thus, the mechanisms of reactive oxygen species-mediated oxidative stress in the pathogenesis of oral lichen planus are numerous and complex. In this review, we first introduce oxidative stress and oxidative products. Then, we summarize the role and possible mechanisms of reactive oxygen species-mediated oxidative stress in the pathogenesis of oral lichen planus and present a clinical correlation between oxidative stress and oral lichen planus. Finally, we discuss the current challenges and future perspectives.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46089599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.1177/1721727X221092900
Zetian Gao, Qiu-bo Zhang, Xie Zhang, Yufei Song
Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive, deadly, and is rarely diagnosed early. Regulatory T cells (Treg) are a multifunctional class of immunosuppressive T cells that help maintain immunologic homeostasis and participate in autoimmune diseases, transplants, and tumors. This cell type mediates immune homeostasis, tolerance, and surveillance and is associated with poor outcomes in PDAC. Tregs remodel the tumor immune microenvironment, mediate tumor immune escape, and promote tumor invasion and metastasis. A promising area of research involves regulating Tregs to reduce their infiltration into tumor tissues. However, the complexity of the immune microenvironment has limited the efficacy of immunotherapy in PDAC. Treg modulation combined with other treatments is emerging. This review summarizes the mechanisms of Tregs activity in tumor immune microenvironments in PDAC and the latest developments in immunotherapy and clinical trials.
{"title":"Advance of T regulatory cells in tumor microenvironment remodeling and immunotherapy in pancreatic cancer","authors":"Zetian Gao, Qiu-bo Zhang, Xie Zhang, Yufei Song","doi":"10.1177/1721727X221092900","DOIUrl":"https://doi.org/10.1177/1721727X221092900","url":null,"abstract":"Pancreatic ductal adenocarcinoma (PDAC) is highly aggressive, deadly, and is rarely diagnosed early. Regulatory T cells (Treg) are a multifunctional class of immunosuppressive T cells that help maintain immunologic homeostasis and participate in autoimmune diseases, transplants, and tumors. This cell type mediates immune homeostasis, tolerance, and surveillance and is associated with poor outcomes in PDAC. Tregs remodel the tumor immune microenvironment, mediate tumor immune escape, and promote tumor invasion and metastasis. A promising area of research involves regulating Tregs to reduce their infiltration into tumor tissues. However, the complexity of the immune microenvironment has limited the efficacy of immunotherapy in PDAC. Treg modulation combined with other treatments is emerging. This review summarizes the mechanisms of Tregs activity in tumor immune microenvironments in PDAC and the latest developments in immunotherapy and clinical trials.","PeriodicalId":55162,"journal":{"name":"European Journal of Inflammation","volume":" ","pages":""},"PeriodicalIF":0.7,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46137983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：