The objectives of this study were a) to analyze the growth of seed origin saplings and young stump sprouts, emerged from small dimension stumps that were established and grew under different shade and site conditions in low elevation Fagus sylvatica L. s.l. stands, which are located in northeastern Greece, and b) to examine whether those stump sprouts can be incorporated in the regeneration process of beech stands growing in analogous marginal, for the species conservation conditions. Fifty-six seed and sprout origin saplings were cut. The sprouts had emerged from stumps having a basal diameter lower than 7 cm. For each of the 56 saplings, the mean annual height increment was calculated, dividing the height of the sapling by the age of its base. The different shade regimes were determined using hemispherical photography. Sprouts, which grew in deep shade in both site types, and in the moderate shade in medium productivity sites, exhibited height growth analogous to seed origin plants growing under moderate and deep shade in good productivity sites, and under moderate shade in medium productivity sites. Moreover, even though it is of no practical significance, stump sprouts that were established and grew under moderate shade in good productivity sites, showed higher height growth rates than all the other (compared) categories of seed and sprout origin saplings. Consequently, sprouts can be used in various types and forms of shelterwood silvicultural systems where regeneration plants are established and grow under shade conditions. A few years after the emergence of sprouts, as regeneration cuttings proceed through the gradual removal of the mother trees, sprouts will probably exhibit high growth rates as a result of the improved light conditions and their pre-existing root system.
{"title":"Height growth of sprouts emerged from small stumps and seed origin saplings under shade, in low elevation Fagus sylvatica L. s.l. stands in Greece","authors":"E. Milios, C. Papalexandris","doi":"10.12657/denbio.082.001","DOIUrl":"https://doi.org/10.12657/denbio.082.001","url":null,"abstract":"The objectives of this study were a) to analyze the growth of seed origin saplings and young stump sprouts, emerged from small dimension stumps that were established and grew under different shade and site conditions in low elevation Fagus sylvatica L. s.l. stands, which are located in northeastern Greece, and b) to examine whether those stump sprouts can be incorporated in the regeneration process of beech stands growing in analogous marginal, for the species conservation conditions. Fifty-six seed and sprout origin saplings were cut. The sprouts had emerged from stumps having a basal diameter lower than 7 cm. For each of the 56 saplings, the mean annual height increment was calculated, dividing the height of the sapling by the age of its base. The different shade regimes were determined using hemispherical photography. Sprouts, which grew in deep shade in both site types, and in the moderate shade in medium productivity sites, exhibited height growth analogous to seed origin plants growing under moderate and deep shade in good productivity sites, and under moderate shade in medium productivity sites. Moreover, even though it is of no practical significance, stump sprouts that were established and grew under moderate shade in good productivity sites, showed higher height growth rates than all the other (compared) categories of seed and sprout origin saplings. Consequently, sprouts can be used in various types and forms of shelterwood silvicultural systems where regeneration plants are established and grow under shade conditions. A few years after the emergence of sprouts, as regeneration cuttings proceed through the gradual removal of the mother trees, sprouts will probably exhibit high growth rates as a result of the improved light conditions and their pre-existing root system.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-10-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47133393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Seeds of numerous trees and shrubs require specific environmental conditions to initiate germination. In the genus Cotoneaster, seeds often remain dormant in the ground for 1–2 years. Therefore, knowledge of the method of breaking seed dormancy under controlled conditions is needed. Various temperatures of stratification and chemical scarification were tested in this work to break deep double seed dormancy under controlled conditions in C. divaricatus, C. horizontalis and C. hupehensis. Fruits were collected in October, when they were fully ripe. The seeds extracted from the fruits were air-dried at room temperature to a moisture content of approximately 10%. Fresh and air-dried seeds were subjected to different variants of cold stratification or warm-cold stratification with different alternating temperatures. The hard seeds of C. hupehensis were also scarified in concentrated sulfuric acid, followed by warm stratification. A seed was counted as germinated when a 1–3 mm long radicle appeared. After stratification (ca. 5% seed germination), seeds were tested for germination and emergence. The dormancy of air-dried C. divaricatus and C. horizontalis seeds was broken most effectively by stratification for 12–16 weeks at 20°/30°C (16/8 hours), followed by 15–18 weeks at 3°C. Scarification in concentrated sulfuric acid for 3 hours followed by warmcold stratification at 20°/30°C (4 weeks) and 3°C (18 weeks) resulted in the highest germination (48%) of C. hupehensis seeds at 3°/20°C (16/8 hours). In our experiment, we demonstrated that the physiological and physical dormancy of Cotoneaster species can be broken under controlled conditions with stratification or stratification combined with scarification methods.
{"title":"Seed dormancy breaking under controlled conditions in ornamental Cotoneaster spp.","authors":"B. Bujarska-Borkowska, J. Suszka","doi":"10.12657/denbio.081.011","DOIUrl":"https://doi.org/10.12657/denbio.081.011","url":null,"abstract":"Seeds of numerous trees and shrubs require specific environmental conditions to initiate germination. In the genus Cotoneaster, seeds often remain dormant in the ground for 1–2 years. Therefore, knowledge of the method of breaking seed dormancy under controlled conditions is needed. Various temperatures of stratification and chemical scarification were tested in this work to break deep double seed dormancy under controlled conditions in C. divaricatus, C. horizontalis and C. hupehensis. Fruits were collected in October, when they were fully ripe. The seeds extracted from the fruits were air-dried at room temperature to a moisture content of approximately 10%. Fresh and air-dried seeds were subjected to different variants of cold stratification or warm-cold stratification with different alternating temperatures. The hard seeds of C. hupehensis were also scarified in concentrated sulfuric acid, followed by warm stratification. A seed was counted as germinated when a 1–3 mm long radicle appeared. After stratification (ca. 5% seed germination), seeds were tested for germination and emergence. The dormancy of air-dried C. divaricatus and C. horizontalis seeds was broken most effectively by stratification for 12–16 weeks at 20°/30°C (16/8 hours), followed by 15–18 weeks at 3°C. Scarification in concentrated sulfuric acid for 3 hours followed by warmcold stratification at 20°/30°C (4 weeks) and 3°C (18 weeks) resulted in the highest germination (48%) of C. hupehensis seeds at 3°/20°C (16/8 hours). In our experiment, we demonstrated that the physiological and physical dormancy of Cotoneaster species can be broken under controlled conditions with stratification or stratification combined with scarification methods.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42116487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Antoni Zięba, W. Różański, Marcin Bukowski, B. Ciesielska, J. Szwagrzyk
{"title":"Distribution and habitat conditions of Pinus cembra forests in the Tatra Mountains","authors":"Antoni Zięba, W. Różański, Marcin Bukowski, B. Ciesielska, J. Szwagrzyk","doi":"10.12657/denbio.081.010","DOIUrl":"https://doi.org/10.12657/denbio.081.010","url":null,"abstract":"","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45647491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pedunculate oak (Quercus robur L.) is an important component of temperate forests in the northern hemisphere. It occurs naturally across Europe and in parts of North Africa, the Balkans, the Urals and the Caucasus. In Poland, it predominantly grows on the plains at ≤700 m a.s.l. The main objective of this study was to determine how different temperature-humidity growth conditions influence dormancy breakage and epicotyl emergence in Q. robur, using growth curve models. We also investigated whether these differences result from changes in the climatic conditions under which the oak populations grow naturally. In this study, we selected four pedunculate oak stands in western and eastern Poland. These sites were characterised by oceanic and continental climates, respectively. Mature acorns were collected in the autumn of 2016 and pretreated for two weeks in the cold (4 °C). The acorns were then sown in plastic pots filled with a peat/sand substrate. Four climatic variants (cold-dry, cold-wet, warm-dry and warm-wet) and a control (average conditions) were used. Epicotyl emergence was monitored daily for 74 days. Epicotyl emergence dynamics (maximum absolute growth rate, lag time, T50), number of days to epicotyl emergence, and cumulative epicotyl emergence were measured or calculated. The acorns from oceanic climates required more intense warm humid conditions for epicotyl emergence than those from continental climates. In contrast, acorns from continental climates had an evolutionary advantage in that their epicotyl emergence occurred both in cold-dry and warm-wet seasons. This indicated that each population was adapted to its local environment.
{"title":"Dynamics of epicotyl emergence of Quercus robur from different climatic regions is strongly driven by post-germination temperature and humidity conditions","authors":"S. Jastrzębowski, J. Ukalska","doi":"10.12657/denbio.081.009","DOIUrl":"https://doi.org/10.12657/denbio.081.009","url":null,"abstract":"Pedunculate oak (Quercus robur L.) is an important component of temperate forests in the northern hemisphere. It occurs naturally across Europe and in parts of North Africa, the Balkans, the Urals and the Caucasus. In Poland, it predominantly grows on the plains at ≤700 m a.s.l. The main objective of this study was to determine how different temperature-humidity growth conditions influence dormancy breakage and epicotyl emergence in Q. robur, using growth curve models. We also investigated whether these differences result from changes in the climatic conditions under which the oak populations grow naturally. In this study, we selected four pedunculate oak stands in western and eastern Poland. These sites were characterised by oceanic and continental climates, respectively. Mature acorns were collected in the autumn of 2016 and pretreated for two weeks in the cold (4 °C). The acorns were then sown in plastic pots filled with a peat/sand substrate. Four climatic variants (cold-dry, cold-wet, warm-dry and warm-wet) and a control (average conditions) were used. Epicotyl emergence was monitored daily for 74 days. Epicotyl emergence dynamics (maximum absolute growth rate, lag time, T50), number of days to epicotyl emergence, and cumulative epicotyl emergence were measured or calculated. The acorns from oceanic climates required more intense warm humid conditions for epicotyl emergence than those from continental climates. In contrast, acorns from continental climates had an evolutionary advantage in that their epicotyl emergence occurred both in cold-dry and warm-wet seasons. This indicated that each population was adapted to its local environment.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48462701","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
This study examined variation of stomatal density in two populations of Quercus petraea (Matt.) Liebl. and two populations of Q. robur L. from northwestern Turkey. Stomatal density was determined in fully expanded and dried leaf samples that were collected from trees under natural conditions. Stomatal densities of Q. petraea and Q. robur varied from 186 to 459 per mm2 (mean value: 333 stomata per mm2) and from 397 to 826 per mm2 (mean value: 517 stomata per mm2), respectively. Significant differences in stomatal density were found between these two oak species in Turkey as well as between populations within species. Strong and significant negative correlations were observed between stomatal density and leaf length within each species and across the species. While in Central Europe Q. petraea occurs in drier environments than Q. robur, in the present study Q. robur populations grow in more arid environments and have smaller leaves and a higher stomatal density than Q. petraea. Stomatal density had negative correlations with each of the other leaf characters apart from sinus width. In addition, the interspecific PST value (0.48) for stomatal density was relatively high compared to the mean genetic differentiation calculated at eight microsatellite loci (FST = 0.030), suggesting different local adaptations of populations. Further studies that include additional populations will be necessary to associate genetic variation at candidate genes with phenotypic and environmental variation.
{"title":"Stomatal density in Quercus petraea and Q. robur natural populations in Northern Turkey","authors":"C. Yücedağ, J. Sanders, M. Musah, O. Gailing","doi":"10.12657/DENBIO.081.007","DOIUrl":"https://doi.org/10.12657/DENBIO.081.007","url":null,"abstract":"This study examined variation of stomatal density in two populations of Quercus petraea (Matt.) Liebl. and two populations of Q. robur L. from northwestern Turkey. Stomatal density was determined in fully expanded and dried leaf samples that were collected from trees under natural conditions. Stomatal densities of Q. petraea and Q. robur varied from 186 to 459 per mm2 (mean value: 333 stomata per mm2) and from 397 to 826 per mm2 (mean value: 517 stomata per mm2), respectively. Significant differences in stomatal density were found between these two oak species in Turkey as well as between populations within species. Strong and significant negative correlations were observed between stomatal density and leaf length within each species and across the species. While in Central Europe Q. petraea occurs in drier environments than Q. robur, in the present study Q. robur populations grow in more arid environments and have smaller leaves and a higher stomatal density than Q. petraea. Stomatal density had negative correlations with each of the other leaf characters apart from sinus width. In addition, the interspecific PST value (0.48) for stomatal density was relatively high compared to the mean genetic differentiation calculated at eight microsatellite loci (FST = 0.030), suggesting different local adaptations of populations. Further studies that include additional populations will be necessary to associate genetic variation at candidate genes with phenotypic and environmental variation.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41803076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Postharvest needle abscission poses a significant challenge to the balsam fir (Abies balsamea L.) Christmas tree industry. Root detachment, and other postharvest handling and transporting factors, lead to a progressive dehydration leading to postharvest needle loss. If this were so, reducing the transpiratory water loss through regulating stomata would be expected to reduce dehydration extending needle retention. This study explored this hypothesis through manipulation of balsam fir stomata using chemical treatments to determine the effect on water uptake, relative water content, and needle abscission. Branches were collected from 70 trees and immediately applied a postharvest treatment of water (control), ABA, fluoridone, BAP, theophylline, potassium nitrate, or dopamine. Stomatal conductance decreased by 27% after application of ABA and increased by 24%, 17%, and 18% by fluoridone, BA, and potassium nitrate, respectively. Consequently, the ABA treatment resulted in a significantly lower water uptake while fluoridone, BAP, and potassium nitrate all increased water uptake. Despite changes in stomatal conductance and water uptake, there were no significant changes in needle retention. Needle abscission commenced after an average of 12.5 days from postharvest treatment application and reached 100% completion after an average of 71.9 days. It is possible to manipulate stomatal conductance and water uptake through chemical methods, but such a manipulation does not guarantee superior needle retention. It is proposed that deficiency of certain hormonal signals originating from roots may play a critical role in postharvest needle abscission.
{"title":"Modifying stomatal conductance delays dehydration but not postharvest needle abscission in Abies balsamea","authors":"Caitlin McCavour, M. MacDonald, R. Lada","doi":"10.12657/DENBIO.081.008","DOIUrl":"https://doi.org/10.12657/DENBIO.081.008","url":null,"abstract":"Postharvest needle abscission poses a significant challenge to the balsam fir (Abies balsamea L.) Christmas tree industry. Root detachment, and other postharvest handling and transporting factors, lead to a progressive dehydration leading to postharvest needle loss. If this were so, reducing the transpiratory water loss through regulating stomata would be expected to reduce dehydration extending needle retention. This study explored this hypothesis through manipulation of balsam fir stomata using chemical treatments to determine the effect on water uptake, relative water content, and needle abscission. Branches were collected from 70 trees and immediately applied a postharvest treatment of water (control), ABA, fluoridone, BAP, theophylline, potassium nitrate, or dopamine. Stomatal conductance decreased by 27% after application of ABA and increased by 24%, 17%, and 18% by fluoridone, BA, and potassium nitrate, respectively. Consequently, the ABA treatment resulted in a significantly lower water uptake while fluoridone, BAP, and potassium nitrate all increased water uptake. Despite changes in stomatal conductance and water uptake, there were no significant changes in needle retention. Needle abscission commenced after an average of 12.5 days from postharvest treatment application and reached 100% completion after an average of 71.9 days. It is possible to manipulate stomatal conductance and water uptake through chemical methods, but such a manipulation does not guarantee superior needle retention. It is proposed that deficiency of certain hormonal signals originating from roots may play a critical role in postharvest needle abscission.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49007458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Szymajda, E. Žurawicz, R. Maciorowski, K. Pruski
The deep dormancy of the Prunus species seeds is caused by the presence of germination inhibitors, mainly abscisic acid (ABA) – in the endocarp, the seed coat and endosperm, and in the embryo. As a consequence, the removal of the endocarp, the seed coat together with the endosperm increases the number of germinated seeds. The effect of different treatments of seeds of three peach cultivars – ‘Madison’, ‘Elberta’ and ‘Rakoniewicka’, and of three apricot cultivars – M II‐42, ‘Bella’ and ‘Somo’, were assessed in terms of seed germination and the growth of obtained young seedlings. Seed treatments involved different duration of stratification period at 5°C and removing endocarp, seed coat with endosperm and the part of cotyledons of the embryo. The best seed/embryo germination, about 96% for the peach cultivars and practically 100% for the apricot cultivars, was obtained by stratifying seeds for 90 days and then removing the seed coat together with the endosperm and subjecting the extracted embryos to a temperature of 20°C. The seedlings obtained from the seeds treated this way have shown good growth. Using these treatments, it was possible to obtain a larger number of apricot and peach seedlings, and thus increase the breeding efficiency of these species.
{"title":"Stratification period combined with mechanical treatments increase Prunus persica and Prunus armeniaca seed germination","authors":"M. Szymajda, E. Žurawicz, R. Maciorowski, K. Pruski","doi":"10.12657/denbio.081.006","DOIUrl":"https://doi.org/10.12657/denbio.081.006","url":null,"abstract":"The deep dormancy of the Prunus species seeds is caused by the presence of germination inhibitors, mainly abscisic acid (ABA) – in the endocarp, the seed coat and endosperm, and in the embryo. As a consequence, the removal of the endocarp, the seed coat together with the endosperm increases the number of germinated seeds. The effect of different treatments of seeds of three peach cultivars – ‘Madison’, ‘Elberta’ and ‘Rakoniewicka’, and of three apricot cultivars – M II‐42, ‘Bella’ and ‘Somo’, were assessed in terms of seed germination and the growth of obtained young seedlings. Seed treatments involved different duration of stratification period at 5°C and removing endocarp, seed coat with endosperm and the part of cotyledons of the embryo. The best seed/embryo germination, about 96% for the peach cultivars and practically 100% for the apricot cultivars, was obtained by stratifying seeds for 90 days and then removing the seed coat together with the endosperm and subjecting the extracted embryos to a temperature of 20°C. The seedlings obtained from the seeds treated this way have shown good growth. Using these treatments, it was possible to obtain a larger number of apricot and peach seedlings, and thus increase the breeding efficiency of these species.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47058321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Paweł Przybylski, J. Kowalczyk, I. Odrzykoski, J. Matras
This study investigates the rates of grafting and planting errors that occur in seed orchards, and evaluates their effects on the genetic diversity and relatedness of genotypes. The study used three clonal Scots pine seed orchards of differing ages and clonal composition located in the forest districts of Susz, Pniewy and Zdrojowa Góra, Poland. Maximum breeding ability within a seed orchard requires isolated from external sources of pollen, and have no alien genotypes inside the orchard. We used 13 isoenzyme markers to determine the genotypic identity of ramets and compared the number of genotypes of the actual current ramet population (W1) with genotypes comprising the originally intended plus trees (designated as W0) to estimate the genotypic assignment error rate per orchard. For both W0 and W1, we calculated the effective number of clones and the relative effective number of clones. Ramet assignment errors were detected in all three seed orchards. Gnotypic errors ranged from 5.8% to 37.7% across orchards. A total of 46 alleles were found, with the mean number of alleles per locus ranging from 2.77 to 3.23. At individual loci, the level of observed heterozygosity was variable. Alien genotypes had negligible effects on seed orchard heterozygosity. The Fst values between seed orchards amounted to 0.6% between Susz and Pniewy and 1.1% between Susz and Zdrojowa Góra. The effect on genetic variation of ramet assignment errors was small and influenced genetic diversity only in the case of the Susz seed orchard. However, our results suggest that negative effects of alien genotypes can occur on breeding value of seeds from analysed seeds orchards.
{"title":"Identyfing alien genotypes and their consequences for genetic variationin clonal seed orchards of Pinus sylvestris L.","authors":"Paweł Przybylski, J. Kowalczyk, I. Odrzykoski, J. Matras","doi":"10.12657/DENBIO.081.005","DOIUrl":"https://doi.org/10.12657/DENBIO.081.005","url":null,"abstract":"This study investigates the rates of grafting and planting errors that occur in seed orchards, and evaluates their effects on the genetic diversity and relatedness of genotypes. The study used three clonal Scots pine seed orchards of differing ages and clonal composition located in the forest districts of Susz, Pniewy and Zdrojowa Góra, Poland. Maximum breeding ability within a seed orchard requires isolated from external sources of pollen, and have no alien genotypes inside the orchard. We used 13 isoenzyme markers to determine the genotypic identity of ramets and compared the number of genotypes of the actual current ramet population (W1) with genotypes comprising the originally intended plus trees (designated as W0) to estimate the genotypic assignment error rate per orchard. For both W0 and W1, we calculated the effective number of clones and the relative effective number of clones. Ramet assignment errors were detected in all three seed orchards. Gnotypic errors ranged from 5.8% to 37.7% across orchards. A total of 46 alleles were found, with the mean number of alleles per locus ranging from 2.77 to 3.23. At individual loci, the level of observed heterozygosity was variable. Alien genotypes had negligible effects on seed orchard heterozygosity. The Fst values between seed orchards amounted to 0.6% between Susz and Pniewy and 1.1% between Susz and Zdrojowa Góra. The effect on genetic variation of ramet assignment errors was small and influenced genetic diversity only in the case of the Susz seed orchard. However, our results suggest that negative effects of alien genotypes can occur on breeding value of seeds from analysed seeds orchards.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-07-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46329015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Staszak, E. Pers-kamczyc, Ewelina A. Klupczyńska, T. Pawłowski
European beech seeds are characterised by deep physiological dormancy and to germinate, they require several weeks of stratification at 3°C. Seed dormancy is under hormonal regulation, but the details of how hormones regulate deep dormancy in trees remain not yet well elucidated. We hypothesised that the mechanism of seed dormancy breaking is differentially regulated according to depth of dormancy. Expression of ABI5 and 14-3-3, members of the abscisic acid pathway, and RGL2, a member of gibberellic acid pathways, were examined at the protein and mRNA levels during dormancy alleviation of beech seeds. Unlike in non-deep dormant seeds, ABI5, 14-3-3 and RGL2 were present during nearly all periods of cold stratification in beech seeds, but during dormancy breaking and germination these proteins nearly disappeared. Relative abundances of ABI5 and 14-3-3 transcripts were the highest in dormant dry seeds, and during stratification it decreased gradually. We suppose that during stratification, de novo translation of proteins on the basis of deposited mRNA occurred. On the base of our research we can conclude that the seed dormancy breaking mechanism differs according to seed’s dormancy depth.
{"title":"Expression of abscisic and gibberellic acid signalling factors in Fagus sylvatica L. seeds during dormancy breaking and germination","authors":"A. Staszak, E. Pers-kamczyc, Ewelina A. Klupczyńska, T. Pawłowski","doi":"10.12657/DENBIO.081.003","DOIUrl":"https://doi.org/10.12657/DENBIO.081.003","url":null,"abstract":"European beech seeds are characterised by deep physiological dormancy and to germinate, they require several weeks of stratification at 3°C. Seed dormancy is under hormonal regulation, but the details of how hormones regulate deep dormancy in trees remain not yet well elucidated. We hypothesised that the mechanism of seed dormancy breaking is differentially regulated according to depth of dormancy. Expression of ABI5 and 14-3-3, members of the abscisic acid pathway, and RGL2, a member of gibberellic acid pathways, were examined at the protein and mRNA levels during dormancy alleviation of beech seeds. Unlike in non-deep dormant seeds, ABI5, 14-3-3 and RGL2 were present during nearly all periods of cold stratification in beech seeds, but during dormancy breaking and germination these proteins nearly disappeared. Relative abundances of ABI5 and 14-3-3 transcripts were the highest in dormant dry seeds, and during stratification it decreased gradually. We suppose that during stratification, de novo translation of proteins on the basis of deposited mRNA occurred. On the base of our research we can conclude that the seed dormancy breaking mechanism differs according to seed’s dormancy depth.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49644906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Korean arborvitae (Thuja koraiensis) is an evergreen shrub or small tree native to Korea and the extreme Changbai Mountain of China threatened by habitat loss. Due to the limited genomic sources of Korean arborvitae, it is important to explore transcriptome to understand this economically important plant. We used RNA-seq technology to characterize the transcriptome of root, stem and leaf in Korean arborvitae, respectively. Based on the bioinformatics analysis of assembled transcriptome data, transcriptional pathways and differentially expressed genes (DEGs) were identified. There were 152.26 million reads generated, and 446,568 unigenes with a mean size of 423.51 nt obtained via de novo assembly. Of these, 204,091 unigenes (45.70%) were further annotated by comparison to public protein databases. A total of 63,495 unigenes (14.22%) were associated into 130 pathways by searching against the KEGG database. DEGs analysis identified 11,890, 5,900 and 10,136 DEGs from the comparison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. Besides, photosynthesis, plant hormone signal transduction and metabolism and biosynthesis of sugar and amino acids were changed in different tissues. We obtained 446,568 unigenes in Korean arborvitae and 11,890, 5,900 and 10,136 DEGs from the comparison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. These results will aid in understanding and carrying out future studies on the molecular basis of Korean arborvitae and contribute to future artificial production and applications.
{"title":"Transcriptomic analysis of different tissues in Korean arborvitae","authors":"Huaizhi Mu, Yuting Gao, Fushan Cheng, Lin Lin, Gerong Wang, Fucai Xia","doi":"10.12657/DENBIO.081.004","DOIUrl":"https://doi.org/10.12657/DENBIO.081.004","url":null,"abstract":"Korean arborvitae (Thuja koraiensis) is an evergreen shrub or small tree native to Korea and the extreme Changbai Mountain of China threatened by habitat loss. Due to the limited genomic sources of Korean arborvitae, it is important to explore transcriptome to understand this economically important plant. We used RNA-seq technology to characterize the transcriptome of root, stem and leaf in Korean arborvitae, respectively. Based on the bioinformatics analysis of assembled transcriptome data, transcriptional pathways and differentially expressed genes (DEGs) were identified. There were 152.26 million reads generated, and 446,568 unigenes with a mean size of 423.51 nt obtained via de novo assembly. Of these, 204,091 unigenes (45.70%) were further annotated by comparison to public protein databases. A total of 63,495 unigenes (14.22%) were associated into 130 pathways by searching against the KEGG database. DEGs analysis identified 11,890, 5,900 and 10,136 DEGs from the comparison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. Besides, photosynthesis, plant hormone signal transduction and metabolism and biosynthesis of sugar and amino acids were changed in different tissues. We obtained 446,568 unigenes in Korean arborvitae and 11,890, 5,900 and 10,136 DEGs from the comparison of root vs. stem, root vs. leaf and stem vs. leaf, respectively. These results will aid in understanding and carrying out future studies on the molecular basis of Korean arborvitae and contribute to future artificial production and applications.","PeriodicalId":55182,"journal":{"name":"Dendrobiology","volume":" ","pages":""},"PeriodicalIF":0.9,"publicationDate":"2019-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46595002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：