Lepisosteiformes, ginglymodian fishes with fusiform bodies and ganoid scales, are a common source of Mesozoic fossil remains. Despite their widespread historical distribution, their diversity in the Alcântara Formation (northeastern Brazil) remains poorly understood, with only one lepisosteid previously identified. In contrast, nearby groups, such as the Santana Group and the Kem Kem Group, host diverse lepisosteiform taxa. This study revises the taxonomy of ganoid scales from the Alcântara Formation using external morphology, microstructure, and ultrastructure analyses. The scales examined exhibit characteristic Lepisosteiformes features, including ganoine on the free field and articulation via ventral and vertical processes. Histological analysis revealed both palaeoniscoid and lepidosteoid scales. Lepidosteoid scales varied significantly in shape, size, and structure based on body position, with smooth ganoine scales dominating the sample, and representing a large, non-gar lepisosteiform (cf. Adrianaichthys). Smaller, ornamented scales were rare and indicative of a lepisosteid taxon. Palaeoniscoid scales, identified as Obaichthys, were the least abundant and showed a prominent median spine; they were of different developmental stages and allowed the inference of the existence of different Obaichthys africanus morphotypes. This study offer many insights regarding the different aspects of lepisosteiform squamation such as histological structure, external morphology, shape, size, and ornamentation. We identified at least three Lepisosteiformes taxa in the Alcântara Formation: a non-gar lepisosteiform (cf. Adrianaichthys), the obaichthyid Obaichthys, and a lepisosteid. These findings highlight similarities between Cenomanian faunal communities in northeastern Brazil and northwestern Africa, expanding our understanding of Lepisosteiformes diversity and extending the known vertical distribution of Obaichthyidae in South America to the Cenomanian. We also propose the existence of two distinct morphotypes of O. africanus.
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