The latest Maastrichtian was an important interval in Earth's history, characterized by global climatic fluctuations and significant biotic transitions. Understanding the climate and vegetation changes during this time is important for reconstructing the climatic perturbations during the pre-end-Maastrichtian mass extinction and elucidating the cause of the extinction. However, detailed reconstructions of terrestrial climate and vegetation changes remain limited due to the uneven preservation of palynomorphs and the scarcity of high-resolution palynological records. In this study, based on high-resolution palynological data from the Furao Formation in the XHY2008 borehole, Jiayin, Heilongjiang Province, Northeast China, three latest Maastrichtian palynological assemblages were identified, in ascending stratigraphic order: (1) the Gabonisporis–Pinuspollenites–Aquilapollenites assemblage; (2) the Laevigatosporites–Monocolpopollenites–Ulmoideipites assemblage; and (3) the Osmundacidites–Pseudointegricorpus–Wodehouseia assemblage. These assemblages provide key insights into the composition of latest Maastrichtian palynoflora and associated climatic changes. The data indicate that the latest Maastrichtian climate was warm and humid (i.e., subtropical) in the mid-latitudes of the Northern Hemisphere, which was typical of the Cretaceous greenhouse climate. However, two cooling and drying events were recognized based on quantitative analyses of the palynomorphs using principal component analysis and ecological affinity statistics. These two transient events involved changes from a subtropical–humid to temperate–subhumid climate in the last 34 kyr of the Cretaceous. These short-term climatic changes might record abrupt climatic perturbations during latest Maastrichtian cooling and enhance our knowledge on the frequency of climatic change.
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