: Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identi fi ed using von Willebrand factor (vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic model group, a hypoxic group treated with low-concentration hucMSC-Exos (75 μ g/mL) and a hypoxic group treated with high-concentration hucMSC-Exos (100 μ g/mL). Cell viability and proliferation were assessed using Cell Counting Kit-8 (CCK-8) assay and EdU (5-ethynyl-2 ′ -deoxyuridine) assay respectively. Expression levels of VEGF-A were evaluated using RT-PCR, western blotting and immuno fl uorescence. Results: Hypoxia signi fi cantly increased hfRMECs ’ viability and proliferation by upregulating VEGF-A levels. The administration of hucMSC-Exos effectively reversed this response, with the high-concentration group exhibiting greater ef fi cacy compared to the low-concentration group. Conclusion: In conclusion, hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells.
{"title":"Inhibition of VEGF-A expression in hypoxia-exposed fetal retinal microvascular endothelial cells by exosomes derived from human umbilical cord mesenchymal stem cells","authors":"JING LI, WANWAN FAN, LILI HAO, YONGSHENG LI, GUOCHENG YU, WEI SUN, XIANQIONG LUO, JINGXIANG ZHONG","doi":"10.32604/biocell.2023.044177","DOIUrl":"https://doi.org/10.32604/biocell.2023.044177","url":null,"abstract":": Objective: This study aimed to investigate the potential of human umbilical cord mesenchymal stem cell (hucMSC)-derived exosomes (hucMSC-Exos) in inhibiting hypoxia-induced cell hyper proliferation and overexpression of vascular endothelial growth factor A (VEGF-A) in immature human fetal retinal microvascular endothelial cells (hfRMECs). Methods: Exosomes were isolated from hucMSCs using cryogenic ultracentrifugation and characterized through various techniques, including transmission electron microscopy, nanoparticle tracking analysis, bicinchoninic acid assays, and western blotting. The hfRMECs were identi fi ed using von Willebrand factor (vWF) co-staining and divided into four groups: a control group cultured under normoxic condition, a hypoxic model group, a hypoxic group treated with low-concentration hucMSC-Exos (75 μ g/mL) and a hypoxic group treated with high-concentration hucMSC-Exos (100 μ g/mL). Cell viability and proliferation were assessed using Cell Counting Kit-8 (CCK-8) assay and EdU (5-ethynyl-2 ′ -deoxyuridine) assay respectively. Expression levels of VEGF-A were evaluated using RT-PCR, western blotting and immuno fl uorescence. Results: Hypoxia signi fi cantly increased hfRMECs ’ viability and proliferation by upregulating VEGF-A levels. The administration of hucMSC-Exos effectively reversed this response, with the high-concentration group exhibiting greater ef fi cacy compared to the low-concentration group. Conclusion: In conclusion, hucMSC-Exos can dose-dependently inhibit hypoxia-induced hyperproliferation and VEGF-A overexpression in immature fetal retinal microvascular endothelial cells.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135563776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2022-11-18DOI: 10.32604/biocell.2021.0xxx
Wei Wang, Yanrong Pei, Shui-Hua Wang, Juan Manuel Gorrz, Yu-Dong Zhang
Since 2019, the coronavirus disease-19 (COVID-19) has been spreading rapidly worldwide, posing an unignorable threat to the global economy and human health. It is a disease caused by severe acute respiratory syndrome coronavirus 2, a single-stranded RNA virus of the genus Betacoronavirus. This virus is highly infectious and relies on its angiotensin-converting enzyme 2-receptor to enter cells. With the increase in the number of confirmed COVID-19 diagnoses, the difficulty of diagnosis due to the lack of global healthcare resources becomes increasingly apparent. Deep learning-based computer-aided diagnosis models with high generalisability can effectively alleviate this pressure. Hyperparameter tuning is essential in training such models and significantly impacts their final performance and training speed. However, traditional hyperparameter tuning methods are usually time-consuming and unstable. To solve this issue, we introduce Particle Swarm Optimisation to build a PSO-guided Self-Tuning Convolution Neural Network (PSTCNN), allowing the model to tune hyperparameters automatically. Therefore, the proposed approach can reduce human involvement. Also, the optimisation algorithm can select the combination of hyperparameters in a targeted manner, thus stably achieving a solution closer to the global optimum. Experimentally, the PSTCNN can obtain quite excellent results, with a sensitivity of 93.65%±1.86%, a specificity of 94.32%±2.07%, a precision of 94.30%±2.04%, an accuracy of 93.99%±1.78%, an F1-score of 93.97%±1.78%, Matthews Correlation Coefficient of 87.99%±3.56%, and Fowlkes-Mallows Index of 93.97%±1.78%. Our experiments demonstrate that compared to traditional methods, hyperparameter tuning of the model using an optimisation algorithm is faster and more effective.
{"title":"PSTCNN: Explainable COVID-19 diagnosis using PSO-guided self-tuning CNN.","authors":"Wei Wang, Yanrong Pei, Shui-Hua Wang, Juan Manuel Gorrz, Yu-Dong Zhang","doi":"10.32604/biocell.2021.0xxx","DOIUrl":"10.32604/biocell.2021.0xxx","url":null,"abstract":"<p><p>Since 2019, the coronavirus disease-19 (COVID-19) has been spreading rapidly worldwide, posing an unignorable threat to the global economy and human health. It is a disease caused by severe acute respiratory syndrome coronavirus 2, a single-stranded RNA virus of the genus Betacoronavirus. This virus is highly infectious and relies on its angiotensin-converting enzyme 2-receptor to enter cells. With the increase in the number of confirmed COVID-19 diagnoses, the difficulty of diagnosis due to the lack of global healthcare resources becomes increasingly apparent. Deep learning-based computer-aided diagnosis models with high generalisability can effectively alleviate this pressure. Hyperparameter tuning is essential in training such models and significantly impacts their final performance and training speed. However, traditional hyperparameter tuning methods are usually time-consuming and unstable. To solve this issue, we introduce Particle Swarm Optimisation to build a PSO-guided Self-Tuning Convolution Neural Network (PSTCNN), allowing the model to tune hyperparameters automatically. Therefore, the proposed approach can reduce human involvement. Also, the optimisation algorithm can select the combination of hyperparameters in a targeted manner, thus stably achieving a solution closer to the global optimum. Experimentally, the PSTCNN can obtain quite excellent results, with a sensitivity of 93.65%±1.86%, a specificity of 94.32%±2.07%, a precision of 94.30%±2.04%, an accuracy of 93.99%±1.78%, an F1-score of 93.97%±1.78%, Matthews Correlation Coefficient of 87.99%±3.56%, and Fowlkes-Mallows Index of 93.97%±1.78%. Our experiments demonstrate that compared to traditional methods, hyperparameter tuning of the model using an optimisation algorithm is faster and more effective.</p>","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7613982/pdf/EMS158588.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10517202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.027103
Xi Yang, Huixian Wu, Chao-Liang Xiong, Bo Zhao, Meilian Liu, J. Qin, Meihai Deng
{"title":"Comprehensive bioinformatics analysis of CYB561 expression in breast cancer: Link between prognosis and immune infiltration","authors":"Xi Yang, Huixian Wu, Chao-Liang Xiong, Bo Zhao, Meilian Liu, J. Qin, Meihai Deng","doi":"10.32604/biocell.2023.027103","DOIUrl":"https://doi.org/10.32604/biocell.2023.027103","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90194699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.027485
Changmei Long, Tingting Yang, Yujie Han, Lizhen Han
{"title":"Effects of Tsukamurella tyrosinosolvens P9 on growth, physiology and antioxdant enzyme of peanut under drought stress and after re-watering","authors":"Changmei Long, Tingting Yang, Yujie Han, Lizhen Han","doi":"10.32604/biocell.2023.027485","DOIUrl":"https://doi.org/10.32604/biocell.2023.027485","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88871101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.025677
Kamila Czubak-Prowizor, M. Swiatkowska
{"title":"Junctional adhesion molecule-A (JAM-A) in gynecological cancers: Current state of knowledge","authors":"Kamila Czubak-Prowizor, M. Swiatkowska","doi":"10.32604/biocell.2023.025677","DOIUrl":"https://doi.org/10.32604/biocell.2023.025677","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87807544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.030379
Yanshuang Zhao, Wanxiu Yang
{"title":"The effects of hormone-mediated PI3K/AKT signaling on spermatogenesis in Sertoli cells","authors":"Yanshuang Zhao, Wanxiu Yang","doi":"10.32604/biocell.2023.030379","DOIUrl":"https://doi.org/10.32604/biocell.2023.030379","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91120609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.024944
Yao Lu, Qiuyue Wang, Caihui Zhang
Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization domain (NOD)-like receptor 3 (NLRP3) were measured by western blotting. Immunohistochemistry and TdT-mediated dUTP nick end labeling (TUNEL) staining were performed to analyze the development of apoptosis and pyroptosis. A significant increase in body weight and blood glucose in the DB group was observed. Myocardial pathological injury, fibrosis, apoptosis, and pyroptosis were more obvious and serious in the DB group. The expression of anti-apoptotic Bcl2 significantly decreased, while the expression levels of pro-apoptotic Bax, caspase-3, and pyroptosis-related proteins, such as cleaved GSDMD, and caspase-1 in the DB group were significantly increased. Pyroptosis and apoptosis were probably the main mechanisms that caused myocardial fibrosis in mice with diabetes.
{"title":"Hyperglycemia-induced myocardial fibrosis may be associated with pyroptosis and apoptosis of cardiomyoctes in diabetic mice","authors":"Yao Lu, Qiuyue Wang, Caihui Zhang","doi":"10.32604/biocell.2023.024944","DOIUrl":"https://doi.org/10.32604/biocell.2023.024944","url":null,"abstract":"Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization domain (NOD)-like receptor 3 (NLRP3) were measured by western blotting. Immunohistochemistry and TdT-mediated dUTP nick end labeling (TUNEL) staining were performed to analyze the development of apoptosis and pyroptosis. A significant increase in body weight and blood glucose in the DB group was observed. Myocardial pathological injury, fibrosis, apoptosis, and pyroptosis were more obvious and serious in the DB group. The expression of anti-apoptotic Bcl2 significantly decreased, while the expression levels of pro-apoptotic Bax, caspase-3, and pyroptosis-related proteins, such as cleaved GSDMD, and caspase-1 in the DB group were significantly increased. Pyroptosis and apoptosis were probably the main mechanisms that caused myocardial fibrosis in mice with diabetes.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90242144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.s0124
{"title":"Abstracts from XXIV Annual Meeting of the Argentinean Society of Biology (SAB)","authors":"","doi":"10.32604/biocell.2023.s0124","DOIUrl":"https://doi.org/10.32604/biocell.2023.s0124","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83052457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.024625
Jessica CRISTINA MAR蚇-LLERA, Carlos AMAURY JIM蒒EZ-C罵DENAS, JES赟 CHIMAL-MONROY
{"title":"Control of tendon cell fate in the embryonic limb: A molecular perspective","authors":"Jessica CRISTINA MAR蚇-LLERA, Carlos AMAURY JIM蒒EZ-C罵DENAS, JES赟 CHIMAL-MONROY","doi":"10.32604/biocell.2023.024625","DOIUrl":"https://doi.org/10.32604/biocell.2023.024625","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81637422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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