Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.027718
{"title":"Analysis of tumor-draining vein secretome: A direct access to tumor-derived extracellular vesicles in surgical lung cancer patients","authors":"","doi":"10.32604/biocell.2023.027718","DOIUrl":"https://doi.org/10.32604/biocell.2023.027718","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"15 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78181883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.s0124
{"title":"Abstracts from XXIV Annual Meeting of the Argentinean Society of Biology (SAB)","authors":"","doi":"10.32604/biocell.2023.s0124","DOIUrl":"https://doi.org/10.32604/biocell.2023.s0124","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"24 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83052457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.023553
K. Sun, Dongqin Wang, Zhiqiang Zhang, Yinlong Huang, Xiaofu Lian, Jia Hua, Jing Zhang, Chaoqun Lian
Columbianetin acetate (CE) is one of the effective components of Angelica pubescens. So far, the specific role and molecular mechanism of CE in pancreatic cancer are not clear. Thus, this study aimed to explore the specific mechanism of CE on pancreatic cancer. The target genes combined with CE were predicted through the PharmMapper database and the 3D molecular structure of CE. Then, the Cancer Genome Atlas (TCGA) and Cistrome data browser (DB) databases were used to screen Meiotic nuclear divisions 1 (MND1)-related genes, transcription factors, and transcription factor data sets, and the intersection of the above data sets. The “limma” package in the R and gene expression profiling interactive analysis (GEPIA) databases were used to analyze the correlation and survival difference between the target genes and MND1 to predict the degree of association between CE and MND1. Western blotting and RT-PCR experiments revealed the regulatory relationship among CE, E2F1, and MND1 at the cellular level. The specific effects of CE on pancreatic cancer cells were explored through CCK8, wound healing, migration, and flow cycle experiments. E2F1, also the predictive transcription factor of MND1, was also the predictive target protein of CE. At the same time, E2F1 and MND1 were closely related in pancreatic tissue. In the cell function experiment, CE and interference with E2F1 expression could reduce the gene and protein expression of MND1, which was closely associated with cell proliferation, migration, and cycle development. Similarly, interfering with the expression of mnd1 can also inhibit the further development of tumor cells. CE may inhibit the development of pancreatic cancer cells by reducing the expression of MND1. This implies that CE may be a potential novel agent for the treatment of pancreatic cancer.
乙酸柱莲素是当归的有效成分之一。迄今为止，CE在胰腺癌中的具体作用和分子机制尚不清楚。因此，本研究旨在探讨CE治疗胰腺癌的具体机制。通过PharmMapper数据库和CE的三维分子结构预测与CE结合的靶基因。然后，利用Cancer Genome Atlas (TCGA)和Cistrome data browser (DB)数据库筛选MND1相关基因、转录因子、转录因子数据集以及上述数据集的交集。利用R和基因表达谱交互分析(GEPIA)数据库中的“limma”包分析靶基因与MND1的相关性和生存差异，预测CE与MND1的关联程度。Western blotting和RT-PCR实验揭示了CE、E2F1和MND1在细胞水平上的调控关系。通过CCK8、伤口愈合、迁移和血流循环实验探讨CE对胰腺癌细胞的特异性影响。E2F1也是MND1的预测转录因子，也是CE的预测靶蛋白。同时，E2F1与MND1在胰腺组织中密切相关。在细胞功能实验中，CE和干扰E2F1表达可降低与细胞增殖、迁移和周期发育密切相关的MND1基因和蛋白表达。同样，干扰mnd1的表达也可以抑制肿瘤细胞的进一步发展。CE可能通过降低MND1的表达来抑制胰腺癌细胞的发展。这表明CE可能是一种潜在的治疗胰腺癌的新型药物。
{"title":"Columbianetin acetate inhibits the occurrence and development of pancreatic cancer cells by down-regulating the expression of Meiotic nuclear divisions 1","authors":"K. Sun, Dongqin Wang, Zhiqiang Zhang, Yinlong Huang, Xiaofu Lian, Jia Hua, Jing Zhang, Chaoqun Lian","doi":"10.32604/biocell.2023.023553","DOIUrl":"https://doi.org/10.32604/biocell.2023.023553","url":null,"abstract":"Columbianetin acetate (CE) is one of the effective components of Angelica pubescens. So far, the specific role and molecular mechanism of CE in pancreatic cancer are not clear. Thus, this study aimed to explore the specific mechanism of CE on pancreatic cancer. The target genes combined with CE were predicted through the PharmMapper database and the 3D molecular structure of CE. Then, the Cancer Genome Atlas (TCGA) and Cistrome data browser (DB) databases were used to screen Meiotic nuclear divisions 1 (MND1)-related genes, transcription factors, and transcription factor data sets, and the intersection of the above data sets. The “limma” package in the R and gene expression profiling interactive analysis (GEPIA) databases were used to analyze the correlation and survival difference between the target genes and MND1 to predict the degree of association between CE and MND1. Western blotting and RT-PCR experiments revealed the regulatory relationship among CE, E2F1, and MND1 at the cellular level. The specific effects of CE on pancreatic cancer cells were explored through CCK8, wound healing, migration, and flow cycle experiments. E2F1, also the predictive transcription factor of MND1, was also the predictive target protein of CE. At the same time, E2F1 and MND1 were closely related in pancreatic tissue. In the cell function experiment, CE and interference with E2F1 expression could reduce the gene and protein expression of MND1, which was closely associated with cell proliferation, migration, and cycle development. Similarly, interfering with the expression of mnd1 can also inhibit the further development of tumor cells. CE may inhibit the development of pancreatic cancer cells by reducing the expression of MND1. This implies that CE may be a potential novel agent for the treatment of pancreatic cancer.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"100 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83348294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.027103
Xi Yang, Huixian Wu, Chao-Liang Xiong, Bo Zhao, Meilian Liu, J. Qin, Meihai Deng
{"title":"Comprehensive bioinformatics analysis of CYB561 expression in breast cancer: Link between prognosis and immune infiltration","authors":"Xi Yang, Huixian Wu, Chao-Liang Xiong, Bo Zhao, Meilian Liu, J. Qin, Meihai Deng","doi":"10.32604/biocell.2023.027103","DOIUrl":"https://doi.org/10.32604/biocell.2023.027103","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90194699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.024944
Yao Lu, Qiuyue Wang, Caihui Zhang
Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization domain (NOD)-like receptor 3 (NLRP3) were measured by western blotting. Immunohistochemistry and TdT-mediated dUTP nick end labeling (TUNEL) staining were performed to analyze the development of apoptosis and pyroptosis. A significant increase in body weight and blood glucose in the DB group was observed. Myocardial pathological injury, fibrosis, apoptosis, and pyroptosis were more obvious and serious in the DB group. The expression of anti-apoptotic Bcl2 significantly decreased, while the expression levels of pro-apoptotic Bax, caspase-3, and pyroptosis-related proteins, such as cleaved GSDMD, and caspase-1 in the DB group were significantly increased. Pyroptosis and apoptosis were probably the main mechanisms that caused myocardial fibrosis in mice with diabetes.
{"title":"Hyperglycemia-induced myocardial fibrosis may be associated with pyroptosis and apoptosis of cardiomyoctes in diabetic mice","authors":"Yao Lu, Qiuyue Wang, Caihui Zhang","doi":"10.32604/biocell.2023.024944","DOIUrl":"https://doi.org/10.32604/biocell.2023.024944","url":null,"abstract":"Myocardial fibrosis is an important manifestation of diabetic cardiomyopathy. This study investigated the potential mechanism of diabetic myocardial fibrosis. Male C57BL/6J and db/db mice aged 8 weeks were randomly divided into the diabetic (DB) and control groups. At 20 weeks, the mouse heart was harvested and subjected to hematoxylin-eosin staining (HE) and Masson staining to investigate the degree of fibrosis. The expressions of transforming growth factor-beta 1 (TGF-β1), collagen-III, B-cell lymphoma-2 (Bcl2), Bcl2-associated X protein (Bax), cleaved gasdermin D (GSDMD), cysteinyl aspartate specific proteinase-1 (caspase-1), apoptosis-associated speck-like protein containing a CARD (ASC), and nucleotide-binding oligomerization domain (NOD)-like receptor 3 (NLRP3) were measured by western blotting. Immunohistochemistry and TdT-mediated dUTP nick end labeling (TUNEL) staining were performed to analyze the development of apoptosis and pyroptosis. A significant increase in body weight and blood glucose in the DB group was observed. Myocardial pathological injury, fibrosis, apoptosis, and pyroptosis were more obvious and serious in the DB group. The expression of anti-apoptotic Bcl2 significantly decreased, while the expression levels of pro-apoptotic Bax, caspase-3, and pyroptosis-related proteins, such as cleaved GSDMD, and caspase-1 in the DB group were significantly increased. Pyroptosis and apoptosis were probably the main mechanisms that caused myocardial fibrosis in mice with diabetes.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"1 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90242144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.029373
HUI LI, CHENGFANG ZHOU, MEI KUANG, YUN LIU, JIEPING CHEN
Fizzy-related protein homolog 1 (FZR1) mainly functions as a specific activator of the anaphase-promoting complex/cyclosome (APC/C) in the cell cycle and controls the G0 and G1 phases of the cell cycle. We highlight recent work that has studied the role of FZR1 in tumorigenesis, growth, differentiation, and genome stability through cell-cycle control. We summarize the current state of knowledge regarding FZR1 structure, function, and the distinct ways of APC/C dysregulation in solid tumors and hematologic malignancies. We also discuss novel approaches for targeting the FZR1 as a cancer therapy and research area for future work.
{"title":"The role of FZR1 in tumorigenesis: Focus on cell-cycle control","authors":"HUI LI, CHENGFANG ZHOU, MEI KUANG, YUN LIU, JIEPING CHEN","doi":"10.32604/biocell.2023.029373","DOIUrl":"https://doi.org/10.32604/biocell.2023.029373","url":null,"abstract":"Fizzy-related protein homolog 1 (FZR1) mainly functions as a specific activator of the anaphase-promoting complex/cyclosome (APC/C) in the cell cycle and controls the G0 and G1 phases of the cell cycle. We highlight recent work that has studied the role of FZR1 in tumorigenesis, growth, differentiation, and genome stability through cell-cycle control. We summarize the current state of knowledge regarding FZR1 structure, function, and the distinct ways of APC/C dysregulation in solid tumors and hematologic malignancies. We also discuss novel approaches for targeting the FZR1 as a cancer therapy and research area for future work.","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"38 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135505236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.027485
Changmei Long, Tingting Yang, Yujie Han, Lizhen Han
{"title":"Effects of Tsukamurella tyrosinosolvens P9 on growth, physiology and antioxdant enzyme of peanut under drought stress and after re-watering","authors":"Changmei Long, Tingting Yang, Yujie Han, Lizhen Han","doi":"10.32604/biocell.2023.027485","DOIUrl":"https://doi.org/10.32604/biocell.2023.027485","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"17 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88871101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01DOI: 10.32604/biocell.2023.023704
Abdul Samad, Kanval Shaukat, M. Ansari, Mereen Nizar, N. Zahra, Ambreen Naz, Hafiz MUHAMMAD WALEED IQBAL, A. Raza, Vladan Pesic, Ivica G. Djalović
{"title":"Role of foliar spray of plant growth regulators in improving photosynthetic pigments and metabolites in Plantago ovata (Psyllium) under salt stress–A field appraisal","authors":"Abdul Samad, Kanval Shaukat, M. Ansari, Mereen Nizar, N. Zahra, Ambreen Naz, Hafiz MUHAMMAD WALEED IQBAL, A. Raza, Vladan Pesic, Ivica G. Djalović","doi":"10.32604/biocell.2023.023704","DOIUrl":"https://doi.org/10.32604/biocell.2023.023704","url":null,"abstract":"","PeriodicalId":55384,"journal":{"name":"Biocell","volume":"3 1","pages":""},"PeriodicalIF":1.2,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89460736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：