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DNA technology and its applications in herpetological research and forensic investigations involving reptiles and amphibians DNA技术及其在爬行动物和两栖动物爬虫学研究和法医调查中的应用
Pub Date : 2008-01-01 DOI: 10.1163/157075408786532057
I. McDowall
DNA-based technologies, in particular those involved with the identification and screening of DNA polymorphisms, have become a major analytical tool for forensic investigators. These technologies were originally designed for crime scene evaluation and analysis and, more specifically, to the identification of individuals linked to crime scenes. However, the same technologies have subsequently been used identify polymorphisms capable of discrimination at the individual or species level in a wide range of vertebrates. These polymorphic markers are relevant to a range of research and investigative applications in reptiles and amphibians including population and conservation studies, phylogenetics and forensic analysis. Initially cost-prohibitive, DNA technology is now within the budget of many non-specialised laboratories and field centres. The advent of PCR-based methodologies has allowed the purification and subsequent profiling of DNA from an impressive array of biological materials, including limited amounts of partially degraded field or forensic samples. In this respect, non-invasive sampling of endangered species in the field is of particular interest. Polymorphisms occurring within regions of the mitochondrial genomes of vertebrates are currently being screened for species-specific identification purposes. Databases are under construction that will allow rapid comparison of matching regions of the genomes of many thousands of animal species — with obvious applications in forensic investigations.
基于DNA的技术,特别是那些涉及鉴定和筛选DNA多态性的技术,已成为法医调查人员的主要分析工具。这些技术最初是为犯罪现场评估和分析而设计的,更具体地说,是为了识别与犯罪现场有关的个人。然而,同样的技术随后被用于在广泛的脊椎动物中识别能够在个体或物种水平上区分的多态性。这些多态性标记与爬行动物和两栖动物的种群和保护研究、系统发育和法医分析等一系列研究和调查应用有关。DNA技术最初成本高昂,但现在已在许多非专业实验室和实地中心的预算之内。基于聚合酶链反应(pcr)的方法的出现使得从一系列令人印象深刻的生物材料中纯化和随后的DNA分析成为可能,包括有限数量的部分降解的野外或法医样本。在这方面,在野外对濒危物种进行非侵入性取样具有特别的意义。在脊椎动物线粒体基因组区域内发生的多态性目前正在筛选用于物种特异性鉴定的目的。数据库正在建设中,它将允许快速比较数千种动物基因组的匹配区域——在法医调查中有明显的应用。
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引用次数: 10
A Microcantilever Sensor Array for the Detection and Inventory of Desert Tortoises 一种用于沙漠象龟探测和盘存的微悬臂传感器阵列
Pub Date : 2008-01-01 DOI: 10.1163/157075408785911048
T. Porter, R. Dillingham, R. Venedam
We have designed and tested a portable instrument consisting of a small infrared camera coupled with an array of piezoresistive microcantilever sensors that is used to provide real-time, non-invasive data on desert tortoise den occupancy. The piezoresistive microcantilever (PMC) sensors are used to obtain a chemical “signature” of tortoise presence from the air deep within the dens, and provide data in cases where the camera cannot extend deep enough into the den to provide visual evidence of tortoise presence. The infrared camera was used to verify the PMC data during testing, and in many cases such as shallower dens, may be used to provide exact numbers on den populations.
我们设计并测试了一种便携式仪器,该仪器由一个小型红外摄像机和一组压阻式微悬臂传感器组成,用于提供沙漠龟穴占用情况的实时、非侵入性数据。压阻式微悬臂(PMC)传感器用于从洞穴深处的空气中获取乌龟存在的化学“特征”,并在摄像机无法深入洞穴的情况下提供数据,以提供乌龟存在的视觉证据。红外摄像机用于在测试过程中验证PMC数据,并且在许多情况下,例如较浅的洞穴,可以用于提供洞穴数量的确切数字。
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引用次数: 2
Records of feral green iguana, Iguana iguana , in Israel 以色列野生绿鬣蜥的记录
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489257
B. Shacham, S. Nemtzov
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引用次数: 3
The red-eared slider, Trachemys scripta elegans, in Israel 以色列的红耳滑鼠,Trachemys scripta elegans
Pub Date : 2008-01-01 DOI: 10.1163/157075408784648817
B. Shacham, O. Hatzofe
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引用次数: 12
Amphibian use of man-made pools on clear-cuts in the Allegheny Mountains of West Virginia, USA 两栖动物在美国西弗吉尼亚州阿勒格尼山脉的空旷地带使用人造水池
Pub Date : 2008-01-01 DOI: 10.1163/157075408784648772
D. S. Barry, T. Pauley, J. Maerz
Timber harvesting can alter habitats of forest-dwelling species and these alterations can be highly detrimental to species such as amphibians that are intimately associated with habitat temperature and moisture regimes. Consequently, information on conservation practices that can reduce impacts on these species can be useful to prevent local extinctions. Twenty-two of 40 pools constructed by the Fernow Experimental Station, USDA Northern Research Station, Parsons, West Virginia in 1993 on primitive haul roads in two clearcut areas on McGowan Mountain, Tucker County, WV were monitored for 3 years to determine their potential use by anurans. Monthly samples (April through September) of water chemistry and amphibian captures were taken to evaluate amphibian use of these pools. Anurans that used the pools to breed were Bufo americanus americanus, Rana sylvatica, Psuedacris brachyphona, and Hyla chrysoscelis. Desmognathus ochrophaeus and Gyrinophilus porphyriticus porphyriticus were also found in ponds but no evidence of breeding was discovered. By 1994, 14 of the 22 pools that held water were used by anurans for reproduction. Species richness of anurans that bred in pools was primarily dependent on pond depth. Man-made small but deep ponds placed carefully such that their hydroperiods are long enough for larvae to complete development (i.e., near seepages) provides breeding habitat for some frogs species, as well as refugia for non-breeding frogs and salamanders in clear-cut areas. Pools constructed at strategic locations on primitive roads have the potential to reduce adverse impacts of timber harvesting on some amphibian populations.
木材采伐可以改变森林栖息物种的栖息地,这些变化对两栖动物等与栖息地温度和湿度制度密切相关的物种非常有害。因此,有关减少对这些物种影响的保护措施的信息对防止当地物种灭绝是有用的。1993年,美国农业部北部研究站,西弗吉尼亚州帕森斯,Fernow实验站在西弗吉尼亚州塔克县麦高恩山的两个空旷地区的原始运输道路上建造了40个水池,其中22个被监测了3年,以确定它们的潜在用途。每月(4月至9月)采集水化学和两栖动物捕获样本,以评估两栖动物对这些水池的使用情况。利用池塘繁殖的无尾目动物有美洲Bufo americanus americanus、林蛙(Rana sylvatica)、短尾目蛙(Psuedacris brachyphona)和黄壳目蛙(Hyla chrysoscelis)。在池塘中也发现了斑点斑蝽和卟啉回蝽,但没有发现繁殖的证据。到1994年,22个蓄水池中有14个被无尾动物用于繁殖。池中繁殖无尾纲动物的物种丰富度主要取决于池深。人造的小而深的池塘精心布置,使它们的水周期足够长,足以让幼虫完成发育(即靠近渗透处),为一些蛙类提供了繁殖的栖息地,也为无繁殖能力的青蛙和蝾螈提供了避难所。在原始道路上的战略位置建造的水池有可能减少伐木对某些两栖动物种群的不利影响。
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引用次数: 10
Exogenous hormones induce poor rates of oviposition in the anurans, Litoria moorei and L. aurea 外源激素诱导无尾蝇、毛蝇和金蝇产卵率低
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489194
R. Browne, G. Gaikhorst, S. Vitali, J. Roberts, P. Matson
Combinations of commercially available hormones (luteinising hormone-realeasing hormone agonist, human chorionic gonadotrophin and progesterone) were given intra-coelomically to gravid females of two Australian bell frogs, Litoria moorei and L. aurea. The injections were not sufficient to cause normal ovulation and egg laying in the absence of males. Further studies are still required to develop a safe method of programming ovulation and oviposition in these and other recalcitrant species to obtain eggs that have not been exposed to sperm.
将市售激素组合(促黄体生成素-释放激素激动剂、人绒毛膜促性腺激素和孕酮)经腔内注射给两只澳大利亚铃蛙,Litoria moorei和L. aurea。在没有雄性的情况下,注射不足以引起正常的排卵和产卵。还需要进一步的研究来开发一种安全的方法来规划这些和其他顽固物种的排卵和产卵,以获得没有暴露于精子的卵子。
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引用次数: 9
Habitat variables influencing breeding effort in northern clade Bufo fowleri: Implications for conservation 影响北方分支福氏蟾蜍繁殖努力的生境变量:对保护的启示
Pub Date : 2008-01-01 DOI: 10.1163/157075408784648853
R. P. Cook, T. Tupper
Local extirpations of the northern clade of Fowler's toad, Bufo fowleri, have been documented in the northeastern United States and Canada. To facilitate conservation of this species, we identified key characteristics of its preferred breeding habitat and adjacent landscape at Cape Cod National Seashore. We conducted calling surveys at 67 wetlands to quantify B. fowleri annual breeding effort over three years. The resultant multivariate models were then tested with data collected at 30 additional wetlands. B. fowleri choruses were more likely to be detected in permanent wetlands than semi-permanent or temporary wetlands. Predaceous fish and Rana catesbeiana did not negatively affect breeding effort. Wetlands used for breeding typically had shallower shores, less emergent vegetation, less canopy cover, fewer organic acids, and were warmer and less acidic than sites with no evidence of breeding choruses. Large choruses of B. fowleri typically occurred in wetlands containing < ∼33% woody emergent vegetation and adjacent uplands had more bare habitat and less development than sites without breeding. Our results suggest that B. fowleri in the northeastern United States will decline as development and post-agrarian reforestation continue and that removal of woody vegetation in and adjacent to breeding ponds may be necessary to maintain some populations.
在美国东北部和加拿大,福勒氏蟾蜍的北部分支Bufo fowleri的局部灭绝已经被记录下来。为了促进该物种的保护,我们确定了其首选繁殖栖息地和科德角国家海岸附近景观的关键特征。我们在67个湿地进行了电话调查,量化了三年来福氏双胞杆菌的年繁殖量。然后用另外30个湿地收集的数据对所得的多变量模型进行了测试。永久湿地比半永久湿地和临时湿地更容易检出福氏芽胞杆菌。掠食性鱼类和鲶鱼对繁殖效果没有负面影响。用于繁殖的湿地通常具有较浅的海岸,较少的新兴植被,较少的树冠覆盖,较少的有机酸,并且比没有繁殖合唱证据的地方更温暖,酸性更低。福氏芽胞菌的大量聚集通常发生在含有< ~ 33%的木本植被的湿地,而邻近的高地比没有繁殖的地方有更多的裸露栖息地和更少的发育。我们的研究结果表明,在美国东北部,随着发展和农业后再造林的继续,福氏B. fowleri将会下降,去除繁殖池内和附近的木本植被可能是维持一些种群的必要条件。
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引用次数: 4
A comparison of saltwater crocodile (Crocodylus porosus) populations in freshwater-floodplain and tidal river habitats of the Adelaide River catchment, Northern Territory, Australia 澳大利亚北领地阿德莱德河流域淡水泛滥平原和潮汐河生境中咸水鳄(Crocodylus porosus)种群的比较
Pub Date : 2008-01-01 DOI: 10.1163/157075408785911066
Mike Letnic
Because they are subject to commercial harvesting, Crocodylus porosus populations in Australia are monitored to detect trends in their population growth. Monitoring programs are restricted primarily to estuarine waters with high crocodile densities, subsequently little information is available on populations in freshwater habitats. In this study I compared the body-size structure and minimum number of C. porosus known to be alive in tidal-estuarine and freshwater-floodplain habitats of the lower Adelaide River catchment, Northern Territory (N.T.) during the 2004 dry season. A total of 669 C. porosus was sighted. Of these individuals, 546 (81.6%) were sighted in tidal-estuarine waters and 123 (18.4%) were sighted in freshwater, floodplain billabongs and creeks. Crocodiles were observed up to 17.3 km from tidal waters. Crocodiles less than 1.2 m total length (TL) were the most frequently sighted size category in both the freshwater-floodplain (68%), and tidal river (31%) populations. Crocodiles 1.2-3.4 m TL comprised 62% of those seen in the tidal river but only 22% of those in freshwater floodplain habitats. Crocodiles >3.4 m TL comprised 10% and 6.5% of the sighted population in freshwater-floodplain and tidal river habitats, respectively. The body-size structure of C. porosus inhabiting the freshwater floodplain and tidal river habitats of the Adelaide River differed markedly from that observed in marine and freshwater river habitats where crocodiles less than 1.2 m are rarely encountered.
由于它们受到商业捕捞的影响,澳大利亚的鳄鱼种群受到监测,以检测其种群增长的趋势。监测计划主要局限于鳄鱼密度高的河口水域,因此淡水栖息地的种群信息很少。在这项研究中,我比较了2004年旱季在北领地(N.T.)阿德莱德河下游集水区潮汐河口和淡水泛滥平原栖息地已知活的C. porosus的体型结构和最小数量。共发现669只棘球蚴。其中546只(81.6%)出现在潮汐河口水域,123只(18.4%)出现在淡水、河滩滩和小溪中。在距潮汐水域17.3公里处发现鳄鱼。总长度小于1.2米(TL)的鳄鱼是淡水泛滥平原(68%)和潮汐河(31%)种群中最常见的体型类别。长1.2-3.4米的鳄鱼占潮汐河中鳄鱼总数的62%,而淡水洪泛区中鳄鱼总数的22%。在淡水泛滥平原和潮汐河生境中,鳄鱼的数量分别占看得见鳄鱼总数的10%和6.5%。生活在阿德莱德河淡水泛滥平原和潮汐河栖息地的C. porosus的体型结构与很少遇到小于1.2 m的海洋和淡水河流栖息地的鳄鱼明显不同。
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引用次数: 3
Reproduction of the Burmese python ( Python molurus bivittatus ) in southern Florida 南佛罗里达的缅甸巨蟒(python molurus bivittatus)的繁殖
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489185
K. Krysko, James C. Nifong, F. Mazzotti, R. Snow, Kevin M. Enge
Kenneth L. Krysko1,5, James C. Nifong1, Ray W. Snow2, Kevin M. Enge3, Frank J. Mazzotti4 1 Florida Museum of Natural History, Division of Herpetology, University of Florida, Gainesville, Florida 32611, USA 2 Everglades National Park, 40001 State Road 9336, Homestead, Florida 33034, USA 3 Florida Fish and Wildlife Conservation Commission, 4005 South Main Street, Gainesville, Florida 32601, USA 4 Department of Wildlife Ecology and Conservation, University of Florida, Fort Lauderdale Research and Education Center, 3205 College Avenue, Davie, Florida 33314, USA 5 Corresponding author; e-mail: kenneyk@flmnh.ufl.edu
Kenneth L. krysko1,5, James C. Nifong1, Ray W. Snow2, Kevin M. Enge3, Frank J. Mazzotti4 1佛罗里达大学佛罗里达自然历史博物馆爬行动物学部,佛罗里达州盖恩斯维尔32611;2大沼泽地国家公园,40001国道9336,佛罗里达州Homestead 33034; 3佛罗里达州鱼类和野生动物保护委员会,佛罗里达州盖恩斯维尔南大街4005号,佛罗里达州32601;劳德代尔堡研究与教育中心,3205学院大道,戴维,佛罗里达州33314,美国5通讯作者;电子邮件:kenneyk@flmnh.ufl.edu
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引用次数: 17
Feeding and reproductive behavior of captive sea snakes Hydrophis cyanocinctus 圈养海蛇的摄食和繁殖行为
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489220
Karthikeyan., Balasubramanian
The annulated sea snake ( Hydrophis cyanocinctus ) is a black and yellow banded neurotoxic hydrophiid, widely distributed along the Indian coast. Observations on the feeding and reproductive behavior of the snake were made for the first time in captivity. It consumed live or chopped fish; prey size depended on snake size. Breeding commenced in October and the matured neonates were observed during the following January. Twenty five gravid snakes gave birth to 87 neonates, i.e. 3-5 neonates per snake. During delivery, they exhibited a different type of movement to release the neonates, and all were delivered only at night. All neonates were measured the morphological traits of weight, snout-vent length, tail length, head length, head width, neck girth and body girth. The relative size of females and males differed significantly. But the relative size of tail length showed an opposite trend compared to other morphological traits. At the end of the experiment, the snakes and neonates were released in the open sea.
环海蛇(Hydrophis cyanocinctus)是一种黑色和黄色的带状神经毒性水螅类动物,广泛分布在印度海岸。首次在人工饲养条件下对该蛇的摄食和繁殖行为进行了观察。它食用活鱼或切碎的鱼;猎物的大小取决于蛇的大小。10月开始繁殖,次年1月观察成熟的幼鸟。25条妊娠蛇共产下87个新生儿,即每条蛇3-5个新生儿。在分娩过程中,它们表现出不同类型的运动来释放新生儿,而且都只在晚上分娩。测定新生儿体重、口鼻长、尾长、头长、头宽、颈围和体围的形态特征。雌性和雄性的相对体型差异显著。而尾长的相对大小则与其他形态性状相反。在实验结束时,这些蛇和幼蛇被释放到大海中。
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引用次数: 2
期刊
Applied Herpetology
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