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A comparison of techniques for sampling amphibians in isolated wetlands in Georgia, USA 美国乔治亚州孤立湿地两栖动物取样技术比较
Pub Date : 2009-01-01 DOI: 10.1163/157075309X12470350858433
Anna L. Farmer, L. Smith, S. Castleberry, J. Gibbons
We compared the effectiveness of five amphibian sampling methods in nine isolated wetlands in Baker County, Georgia, USA. Overall, aquatic funnel traps yielded the most species, although the number detected using frogloggers (automated frog call recording devices), funnel traps, dipnetting, and PVC pipe refugia was not significantly different among sampling techniques. We detected the same median number of species with funnel traps and frogloggers as with all five methods combined. Methods varied widely in their detection probabilities for individual species and life stages. Species occupancy estimates were strongly affected by method choice. Our results suggest that a combination of methods and prolonged sampling periods are necessary to detect the large number of species present in southeastern isolated wetlands. We recommend that future amphibian surveys in these habitats use a combination of floating funnel traps, frogloggers, and crayfish traps as sampling methods when an assessment of species richness is the objective of a study.
在美国乔治亚州贝克县的9个孤立湿地中,比较了5种两栖动物取样方法的有效性。总体而言,水生漏斗陷阱捕获的物种最多,尽管使用青蛙记录器(自动青蛙呼叫记录设备)、漏斗陷阱、浸液和PVC管避难所检测到的物种数量在采样技术之间没有显著差异。我们用漏斗诱捕器和蛙捕器检测到的中位数物种数量与这五种方法的总和相同。不同的方法对不同的物种和生命阶段的检测概率差别很大。物种占用率估计受方法选择的强烈影响。我们的研究结果表明,要检测东南部孤立湿地中存在的大量物种,需要结合多种方法和延长采样周期。我们建议未来在这些栖息地的两栖动物调查中,当评估物种丰富度是一项研究的目标时,使用浮动漏斗陷阱、蛙人陷阱和小龙虾陷阱的组合作为采样方法。
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引用次数: 17
Optimising the sampling of skinks using artificial retreats based on weather conditions and time of day 根据天气条件和时间,利用人工撤退优化石龙子的采样
Pub Date : 2009-01-01 DOI: 10.1163/157075309X12531848432985
J. M. Hoare, Dorothee Hodapp, I. Westbrooke, M. Lettink, C. O'donnell
Artificial retreats are a low-cost, low-impact and effective technique for detecting cryptic taxa, such as herpetofauna, and have potential as a monitoring tool. The success of using artificial retreats for monitoring is dependent on our ability to maximise detection and minimise variability in sightings within a sampling period. Because ectotherms use retreats for their thermal properties, it may be possible to identify weather conditions during which animals consistently use retreats and design a monitoring protocol based on optimal conditions. We investigated the use of artificial retreats by common skinks, Oligosoma polychroma, in mixed grassland habitat in southern New Zealand under various weather conditions and at a range of times of day. We recorded 1175 sightings of skinks during 1800 artificial retreat checks over a nine day period in early summer 2008. Ambient temperature, rainfall, humidity and time of day were the strongest predictors of skink sightings beneath retreats. Skink sightings were highest and least variable: (1) in an ambient temperature range of 12-18°C, (2) during light or no rain, and (3) at low levels of relative humidity. Under optimal weather conditions, skink counts were similar during the day (8 a.m. to 5 p.m. inclusive) but lower during the evening (8 p.m.). Observer variability, type of substrate beneath the retreat and retreat colour did not significantly influence the number of skinks sighted. We highlight the need to optimise protocols for checking artificial retreats on a species- and site-specific basis before attempting to evaluate their use as a monitoring tool for herpetofauna.
人工退群是一种低成本、低影响、有效的隐蔽类群探测技术,具有潜在的监测潜力。使用人工撤退进行监测的成功取决于我们在采样周期内最大限度地检测和最小化目击变化的能力。由于变温动物利用休养所来提高它们的热性能,因此有可能确定动物持续使用休养所的天气条件,并根据最佳条件设计监测方案。我们调查了新西兰南部混合草地栖息地中常见石龙子(Oligosoma polychroma)在不同天气条件和一天中不同时间的人工撤退。2008年初夏,在为期9天的1800次人工撤退检查中,我们记录了1175次石龙子目击事件。环境温度,降雨量,湿度和一天中的时间是在撤退下发现石龙子的最强预测因子。(1)在12-18°C的环境温度范围内,(2)在下雨或无雨的情况下,(3)在相对湿度较低的情况下,石龙子的目击率最高,变化最小。在最佳天气条件下,石龙子的数量在白天(早上8点到下午5点包括在内)相似,但在晚上(晚上8点)较低。观察者的可变性、后退的底物类型和后退的颜色对看到的石龙子数量没有显著影响。我们强调,在试图评估人工撤退作为一种监测爬虫动物的工具之前,需要在特定物种和地点的基础上优化检查人工撤退的方案。
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引用次数: 32
Methods in herpetological forensic work — clinical techniques 爬行动物法医工作方法。临床技术
Pub Date : 2009-01-01 DOI: 10.1163/157075408X386141
Mark A. Mitchell, S. Hernandez-Divers
Biologists, law enforcement officials and veterinarians are routinely called upon to investigate reptile cases for abuse, neglect, illegal importation, and abandonment. While pursuing these situations, it is important that evidence is collected in an organized and systematic way to ensure successful prosecution or to mount a defense. There are different types of evidence that can be amassed to diagnose a disease/condition in a reptile case. Antemortem clinical investigations can be conducted for those cases where the animals are alive, while postmortem examinations should be pursued for animals that have expired. The purpose of this article is to review the common antemortem clinical techniques that can be used for forensic cases. There are a number of clinical diagnostics available for the forensic case, including the physical examination, clinical pathology, parasite diagnostics, infectious disease diagnostics, clinical toxicology, and diagnostic imaging. In addition to the clinical techniques, it is important to review and document the methods used to house and care for the animals. For this, a thorough review of the husbandry practices provided for the animal is needed.
生物学家、执法官员和兽医经常被要求调查虐待、忽视、非法进口和遗弃爬行动物的案件。在处理这些情况时,重要的是要以有组织和系统的方式收集证据,以确保成功起诉或进行辩护。在爬行动物病例中,可以收集不同类型的证据来诊断疾病/病症。在动物还活着的情况下,可以进行死前临床调查,而在动物已经死亡的情况下,应该进行死后检查。本文的目的是回顾常见的临死前临床技术,可用于法医案件。有许多临床诊断可用于法医案件,包括体格检查、临床病理学、寄生虫诊断、传染病诊断、临床毒理学和诊断成像。除了临床技术,重要的是审查和记录用于饲养和照顾动物的方法。为此,需要对提供给动物的饲养方法进行彻底的审查。
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引用次数: 2
Conservation of the herpetofauna of the Dominican Republic 多米尼加共和国的爬行动物保护
Pub Date : 2009-01-01 DOI: 10.1163/157075409X427153
R. Powell, Sixto J. Incháustegui
The hcrpetofauna of the Dominican Republic consists of 39 frogs (two of which are introduced), 110 squa­ mates (olle possibly extinct and three or fOUf introduced), one crocodilian, three turtles (one introduced), plus fOUf species of sea turtles. Renccting the recent "Glohal Amphibian Assessment", 32 of 37 (86%) on· live species of amphibians are included on the IUeN Red List. Reptilian species given formal recognition as being in need of protection include the sea turtles (listed in CITES appendices and the IUCN Red List), the two native species of pond turtles (Traehelll)'s spp.: mCN, although one as being at "lower risk" ofextinc~ tion), both species of rock iguanas (Cyclfll"ll spp.; CITES and IUCN), two giant galJiwasps (CeleslIu spp., meN), three boids (Epierates spp., CITES), a ground boa (Tropidophis haetiamls, CITES), and the Amer­ ican crocodile (Crocodyills aelltlls; CITES and IUCN). However, at least some additional squamate species appear to meet criteria for inclusion on the IUeN Red List. Four factors largely responsible for the status of these species arc: (I) small ranges, habitat specialization. and encroachment by human activities (many amphibians); (2) large size and cconomic value (turtles. iguanas, crocodile): (3) persecution by people who fear them (galliwasps and snakes); and (4) diurnally active, terrestrial. and vulnerable to predation by mon­ gooses and other introduced mammalian predators (some snakes, Mabllya). Although protection for a few species and for national parks in critical habitats is legislated, enforcement is sporadic and threats, mostly associated with exploitation and development, remain. Specific recommendations for the conservation of the herpetofauna arc listed. © Koninklijke Brill NV. Lciden. 2009
多米尼加共和国的爬虫动物群包括39种青蛙(其中两种是引进的),110种方头蛙(可能灭绝,三种或四种引进的),一种鳄鱼,三种海龟(一种引进的),以及四种海龟。根据最近的“全球两栖动物评估”,37种两栖动物中有32种(86%)被列入IUeN红色名录。被正式认可为需要保护的爬行动物物种包括海龟(列入CITES附录和IUCN红色名录),两种本地池塘龟(特拉海)的物种:mCN,尽管其中一种处于灭绝的“较低风险”),两种岩鬣蜥(特拉海)的物种;),两种巨型大黄蜂(CeleslIu spp., meN),三种鳄鱼(Epierates spp., CITES),一种地蟒(troidophis haetiamls, CITES)和美洲鳄鱼(Crocodyills aelltls;CITES和IUCN)。然而,至少有一些其他的鳞类动物似乎符合列入IUeN红色名录的标准。造成这些物种现状的主要因素有四个:(1)分布范围小,生境特化。人类活动的侵犯(许多两栖动物);(2)体积大,经济价值高(龟)。鬣蜥、鳄鱼);(3)被害怕它们的人(蜥蜴和蛇)迫害;(4)日活动,陆生。而且容易受到母鹅和其他引进的哺乳动物捕食者(一些蛇,马布利亚)的捕食。虽然立法保护少数物种和国家公园的关键栖息地,但执法是零星的,威胁仍然存在,主要与开发和开发有关。还列出了保护爬行动物的具体建议。©Koninklijke Brill NV. Lciden. 2009
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引用次数: 12
Forensic Science and Herpetology 法医学和爬虫学
Pub Date : 2008-11-01 DOI: 10.1163/157075408786532084
J. Cooper, B. W. Baker, M. Cooper
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引用次数: 0
Abnormalities of forelimb and pronephros in a direct developing frog suggest a retinoic acid deficiency 直接发育的青蛙前肢和前肾异常提示维甲酸缺乏
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489202
R. Elinson, Seung Yun Lee
The direct developing frog, Eleutherodactylus coqui, serves as a model for a divergent, derived pattern of anuran development. Reproduction and development occur normally in captivity, although individual embryos and sometimes entire clutches exhibit edema. Edematous embryos have abnormal pronephroi, as judged by histology and by alkaline phosphatase staining. The pronephric defects would be sufficient to account for the edema. The first indicator of later edema is loss or reduced development of the forelimbs. The coupling of forelimb and pronephric abnormalities is likely due to insufficient retinoic acid. The same syndrome can be produced experimentally by treatment with citral, an inhibitor of retinoic acid synthesis. The involvement of retinoic acid in forelimb development, found previously in other vertebrates, can now be extended to anuran amphibians. Reduced forelimb development in embryos may be a useful way to detect insufficient dietary vitamin A in reproductive colonies of direct developing frogs and of other vertebrates.
直接发育的沙蛙,coqui沙蛙,是无尾猿发展的发散衍生模式的一个模型。繁殖和发育在圈养环境中正常进行,尽管个别胚胎和有时整个卵窝出现水肿。通过组织学和碱性磷酸酶染色判断,水肿胚胎肾原异常。肾原缺损足以解释水肿的原因。晚期水肿的第一个指标是前肢丧失或发育减少。前肢和肾原异常的耦合可能是由于维甲酸不足造成的。实验中,用柠檬酸(一种维甲酸合成抑制剂)治疗也会产生同样的综合征。维甲酸参与前肢发育,以前在其他脊椎动物中发现,现在可以扩展到无脊椎两栖动物。胚胎前肢发育减少可能是检测直接发育的青蛙和其他脊椎动物生殖群体饮食中维生素a不足的有用方法。
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引用次数: 8
Low thermal tolerances of stream amphibians in the Pacific Northwest: Implications for riparian and forest management 西北太平洋地区溪流两栖动物的低耐热性:对河岸和森林管理的影响
Pub Date : 2008-01-01 DOI: 10.1163/157075408783489211
R. Bury
Temperature has a profound effect on survival and ecology of amphibians. In the Pacific Northwest, timber harvest is known to increase peak stream temperatures to 24 ◦ C or higher, which has potential to negatively impact cold-water stream amphibians. I determined the Critical Thermal Maxima (CTmax) for two salamanders that are endemic to the Pacific Northwest. Rhyacotriton variegatus larvae acclimated at 10 ◦ C had mean CTmax of 26.7 ± 0. 7S D ◦ C and adults acclimated at 11 ◦ C had mean CTmax of 27.9 ± 1.1 ◦ C. These were among the lowest known values for any amphibian. Values were significantly higher for larval Dicamptodon tenebrosus acclimated at 14 ◦ C ( ¯ x = 29.1 ± 0.2 ◦ C). Although the smallest R. variegatus had some of the lowest values, size of larvae and adults did not influence CTmax in this species. Current forest practices retain riparian buffers along larger fish-bearing streams; however, such buffers along smaller headwaters and non-fish bearing streams may provide favorable habitat conditions for coldwater-associated species in the Pacific Northwest. The current study lends further evidence to the need for protection of Northwest stream amphibians from environmental perturbations. Forest guidelines that include riparian buffer zones and configurations of upland stands should be developed, while monitoring amphibian responses to determine their success.
温度对两栖动物的生存和生态有着深远的影响。在太平洋西北地区,木材采伐已知会将溪流的峰值温度提高到24°C或更高,这可能会对冷水溪流两栖动物产生负面影响。我确定了太平洋西北地区特有的两种蝾螈的临界温度最大值(CTmax)。10℃环境下驯化的异纹螟幼虫CTmax平均值为26.7±0。7S D◦C和适应于11◦C的成人的平均CTmax为27.9±1.1◦C,这是所有两栖动物中已知的最低值之一。在14°C(¯x = 29.1±0.2°C)环境下驯化的斑齿龙幼虫CTmax值显著高于黄齿龙(Dicamptodon tenebrosus),尽管最小的斑齿龙的CTmax值最低,但幼虫和成虫的大小对其CTmax没有影响。目前的森林做法保留了较大的鱼类溪流沿岸的河岸缓冲带;然而,沿着较小的源头和非鱼类溪流的缓冲带可能为太平洋西北部冷水相关物种提供有利的栖息地条件。目前的研究进一步证明了保护西北河流两栖动物免受环境干扰的必要性。应制定包括河岸缓冲区和高地林分配置在内的森林指南,同时监测两栖动物的反应,以确定其成功与否。
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引用次数: 43
Technology meets tradition: A combined VIE-C technique for individually marking anurans 技术与传统的结合:一种单独标记anurans的VIE-C组合技术
Pub Date : 2008-01-01 DOI: 10.1163/157075408785911002
Kristine Hoffmann, Monica E. McGarrity, Steve A. Johnson
We report on the use of a hybrid marking technique (VIE-C) combining Visible Implant Elastomer (VIE) marks with toe-clipping (C) to mark individuals of several species of treefrogs (Hylidae). Our marking strategy entailed injecting elastomer into the plantar surface of the digits and clipping only one toe. This method allows large numbers of frogs to be individually marked, reduces the potential for negative effects due to clipping multiple toes, and minimizes the frequency of elastomer migration from the injection site, a common problem with VIE marks on the body or limbs. We found retention rate of VIE marks in the digits to be similar to that of toe-clips, indicating that VIE provides a satisfactory alternative to multiple toe-clips. In addition, cost of materials, frog handling time, and ill effects were minimal. This VIE-C marking scheme is highly recommended when considering techniques for marking anurans, as it reduces potential negative effects of clipping multiple toes, and provides a large number of inexpensive and long-lasting individual marks that can be easily applied and quickly read in the field by trained observers.
本文报道了一种结合可见植入弹性体(VIE)标记和趾夹(C)标记的杂交标记技术(VIE-C),用于标记几种树蛙(Hylidae)的个体。我们的标记策略是将弹性体注射到手指的足底表面,只剪掉一个脚趾。这种方法允许对大量蛙类进行单独标记,减少了由于夹断多个脚趾而产生的潜在负面影响,并最大限度地减少了弹性体从注射部位迁移的频率,这是身体或四肢上VIE标记的常见问题。我们发现VIE标记在手指上的保留率与脚趾夹的保留率相似,表明VIE提供了一个令人满意的替代多个脚趾夹。此外,材料成本、青蛙处理时间和不良影响都很小。在考虑标记anurans的技术时,强烈推荐使用这种viec标记方案,因为它减少了修剪多个脚趾的潜在负面影响,并提供了大量廉价且持久的单个标记,可以由训练有素的观察者在现场轻松应用和快速读取。
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引用次数: 27
Scientific refutation of traditional Chinese medicine claims about turtles 科学驳斥中医关于乌龟的说法
Pub Date : 2008-01-01 DOI: 10.1163/157075408784648835
Hong Meiling, Shi Haitao, Fu Lirong, Gong Shi-ping, J. Fong, J. F. Parham
The Chinese turtle trade is the primary threat to endangered turtle populations throughout Asia, primarily because of the long tradition of consuming turtles in China. Practitioners of Traditional Chinese Medicine (TCM) promote nutritional and medicinal benefits from eating turtles, especially those made from hardshell species. We tested these claims by determining the nutritional value of turtle products (meat, fat and shell) in five species of geoemydid turtle, Cuora trifasciata, C. mouhotii, Mauremys mutica, M. sinensis and Geoemyda spengleri. Nutritional variables such as the composition of amino acids, fatty acids and mineral elements were analyzed to determine the relative nutritional quality of turtle products. Our study refutes TCM claims about products made from hardshell turtles. Alternative animal products should be substituted to obtain similar minerals, amino acids and fatty acids. Balancing the cultural use of turtles with their conservation status remains a major challenge.
中国海龟贸易是整个亚洲濒危海龟种群的主要威胁,主要是因为中国有悠久的食用海龟的传统。中医医生提倡食用海龟,尤其是那些由硬壳物种制成的海龟,具有营养和药用价值。我们通过测定五种地鳖(Cuora trifasciata, C. mouhotii, Mauremys mutica, M. sinensis和geemyda spengleri)龟产品(肉、脂肪和壳)的营养价值来验证这些说法。通过对氨基酸、脂肪酸和矿质元素组成等营养变量的分析,确定了龟产品的相对营养品质。我们的研究驳斥了中医对由硬壳龟制成的产品的说法。应该用替代动物产品来获得类似的矿物质、氨基酸和脂肪酸。平衡海龟的文化用途和它们的保护状况仍然是一项重大挑战。
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引用次数: 14
Translocation of the Gopher Tortoise: Difficulties Associated with Assessing Success 地鼠龟的易位:与评估成功相关的困难
Pub Date : 2008-01-01 DOI: 10.1163/157075408784648844
S. Riedl, H. Mushinsky, E. McCoy
Gopher tortoises on lands to be developed may be translocated as a conservation measure, sometimes to areas already occupied by the species. We assessed the success of this type of translocation by monitoring the movements, spatial positioning, health, and reproductive activity of translocated and resident individuals at a site in central Florida from 2001 to 2004. By several criteria, the translocation was a success. Most translocated individuals remained on-site for at least one year, home ranges of resident individuals were not significantly different before and after translocation, home ranges of translocated individuals fit within the range of estimates reported in the literature, and neither body condition nor reproduction of either group of individuals could be shown to be affected by the translocation. On the other hand, several resident individuals altered their habitat use after translocation and the spatial positioning of resident individuals was different than that of individuals throughout; so, some potential exists for future off-site movements. The study illustrates two practical problems in assessing translocation success: lack of adequate pre-translocation data for both resident and translocated individuals, which interferes with documentation of translocation effects, and the necessarily small sample sizes, which reduces statistical power.
作为一项保护措施,待开发土地上的地鼠龟可能会被转移,有时会转移到该物种已经占据的地区。从2001年到2004年,我们通过监测迁移个体和常住个体的移动、空间定位、健康和生殖活动来评估这种类型迁移的成功。从几个标准来看,这次易位是成功的。大多数易位个体在易位前后的居住范围没有显著差异,易位个体的居住范围符合文献报道的估计范围,两组个体的身体状况和繁殖均未受到易位的影响。另一方面,部分常住个体在迁移后改变了其栖息地利用方式,且常住个体的空间定位与整体个体的空间定位存在差异;因此,未来的非现场移动存在一些潜力。该研究说明了评估易位成功的两个实际问题:缺乏足够的居民和易位个体的易位前数据,这干扰了对易位效应的记录,以及必要的小样本量,这降低了统计能力。
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引用次数: 21
期刊
Applied Herpetology
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