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Aquaporin OsPIP2;2 Regulates Bakanae Disease Resistance in Rice 水通道蛋白OsPIP2;2调控水稻对Bakanae病的抗性

IF 6.1 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-07-01 DOI: 10.1016/j.rsci.2025.01.006

Xin Liu , Chanyuan Li , Ling Chen , Younan Ouyang , Zhijuan Ji

引用次数: 0

Genome-Wide Association Study and Haplotype Analysis Jointly Identify New Candidate Genes for Alkaline Tolerance at Seedling Stage in Rice 全基因组关联研究与单倍型分析联合鉴定水稻苗期耐碱性新候选基因

IF 6.1 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-07-01 DOI: 10.1016/j.rsci.2025.04.006

Ratan Kumar Ganapati , Kai Chen , Xiuqin Zhao , Tianqing Zheng , Fan Zhang , Laiyuan Zhai , Jianlong Xu

Alkaline soil is characterized by high soluble salt content, elevated pH levels, and ionic imbalance, all of which collectively intensify the harmful effects of alkaline stress on plants. To gain molecular insights into alkaline tolerance (AT), we evaluated 13 AT-related traits in 508 diverse rice accessions from the 3K Rice Germplasm Project at the seedling stage. A total of 2 929 764, 2 059 114, and 1 365 868 single nucleotide polymorphisms were used to identify alkaline-tolerance QTLs via genome-wide association studies (GWAS) in the entire population as well as in the xian and geng subpopulations, respectively. Candidate genes and their superior haplotypes were further identified through gene-based association, haplotype analysis, and gene function annotation. In total, 99 QTLs were identified for AT by GWAS, and three genes (LOC_Os03g49050 for qSSD3.1, LOC_Os05g48760 for qSKC5, and LOC_Os12g01922 for qSNC12) were selected as the most promising candidate genes. Furthermore, we successfully mined superior alleles of key candidate genes from natural variants associated with AT-related traits. This study identified crucial candidate genes and their favorable alleles for AT traits, laying a foundation for further gene cloning and the development of AT rice varieties via marker-assisted selection.

碱性土壤具有可溶性盐含量高、pH值升高、离子失衡等特征，这些特征共同加剧了碱胁迫对植物的有害影响。为了深入了解碱性耐受性（AT）的分子机制，我们对来自3K水稻种质资源项目的508份不同水稻材料幼苗期的13个AT相关性状进行了分析。通过全基因组关联研究（GWAS），在全群体、西安亚群体和耿亚群体中分别鉴定了2 929 764、2 059 114和1 365 868个单核苷酸多态性。通过基因关联、单倍型分析和基因功能注释进一步鉴定候选基因及其优势单倍型。GWAS共鉴定出99个AT qtl，筛选出qSSD3.1的LOC_Os03g49050、qSKC5的LOC_Os05g48760和qSNC12的LOC_Os12g01922为最有希望的候选基因。此外，我们成功地从与at相关性状相关的自然变异中挖掘出关键候选基因的优越等位基因。本研究确定了AT性状的关键候选基因及其有利等位基因，为进一步通过标记辅助选择进行基因克隆和AT水稻品种开发奠定了基础。

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引用次数: 0

A Recombinase-Aided Amplification-Lateral Flow Dipstick Detection Technique for Early On-Site Diagnosis of Bacterial Blight Caused by Xanthomonas oryzae pv. oryzae in Rice 重组酶辅助扩增-侧流试纸检测技术在水稻黄单胞菌疫病早期现场诊断中的应用。水稻中的稻谷

IF 6.1 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-07-01 DOI: 10.1016/j.rsci.2025.01.005

Yuxuan Hou , Jie Zhu , Chenglong Lu , Libo Fan , Mengqi Liang , Xiaobo Zhang , Benyi Cheng , Xia Xu , Junyi Gong

Bacterial blight (BB) is a devastating worldwide rice disease caused by Xanthomonas oryzae pv. oryzae (Xoo), which is difficult to diagnose based on early symptoms. Conventional chemical control yields limited effectiveness once BB has spread. Consequently, it is imperative to develop a rapid, highly sensitive, specific, and easy-to-use detection technique for early on-site diagnosis of BB. We first developed a recombinase-aided amplification-lateral flow dipstick (RAA-LFD) technique for the on-site detection of Xoo. The optimized reaction temperature and time were 37 ºC and 20 min, indicating that the reaction system can be initiated by body temperature independently of any precision instruments. Evaluation of the RAA-LFD technique using the primers (RAAF2/R2) and probe (RAA2-nfo-probe) derived from the XooORF0080 locus exhibited high specificity and eliminated cross-reactivity with other bacterial species. The sensitivity of RAA-LFD is up to 1 pg/μL for Xoo genomic DNA and 100 CFU/mL for Xoo cells. Significantly, this technique accurately detected Xoo from both artificially inoculated and naturally infected rice leaves at the early stage of infection, directly deploying plant tissue fluid as the template without DNA extraction. These attributes make the developed RAA-LFD system a viable technique for the early diagnosis of BB in the field, providing technical support for early-warning systems and disease control.

白叶枯病（BB）是由水稻黄单胞菌（Xanthomonas oryzae pv）引起的一种世界性的水稻病害。这种病很难根据早期症状进行诊断。一旦BB扩散，常规的化学防治效果有限。因此，开发一种快速、高灵敏度、特异度和易于使用的检测技术对BB进行早期现场诊断势在必行。我们首先开发了一种重组酶辅助扩增-横向流动量尺（RAA-LFD）技术用于Xoo的现场检测。优化的反应温度为37℃，反应时间为20 min，表明该反应体系可由体温启动，无需任何精密仪器。利用XooORF0080基因座的引物（RAAF2/R2）和探针（raa2 - info -probe）对RAA-LFD技术进行评价，结果表明该技术具有较高的特异性，并且消除了与其他细菌的交叉反应性。RAA-LFD对Xoo基因组DNA的灵敏度可达1 pg/μL，对Xoo细胞的灵敏度可达100 CFU/mL。值得注意的是，该技术在侵染早期从人工接种和自然侵染的水稻叶片中都能准确检测到Xoo，直接利用植物组织液作为模板，无需提取DNA。这些特点使所开发的RAA-LFD系统成为现场早期诊断BB的可行技术，为预警系统和疾病控制提供技术支持。

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引用次数: 0

A Comprehensive Review of Hierarchical Porous Carbon Synthesis from Rice Husk 稻壳分级多孔碳合成技术综述

IF 6.1 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-07-01 DOI: 10.1016/j.rsci.2025.04.009

Dinuka Nuwan Tharaka , Nadeeka D. Tissera , Gayan Priyadarshana , Damayanthi Dahanayake

Hierarchical porous carbon (HPC) materials exhibit superior performance profiles in various applications due to their well-developed multiscale interconnected pore structures. The synthesis of HPC from natural biomass precursors instead of fossil fuel-based precursors has gained considerable attention in recent decades. Rice husk, a globally abundant agricultural waste, offers a sustainable and cost-effective precursor for HPC production. The structural components and inherent silica content of rice husk act as a natural self-template for forming hierarchical pore structures with superior characteristics. In this review, recent studies on preparing rice husk-based HPC are summarized, and synthesis techniques are evaluated. In addition, recent advancements in activation methods and the effect of silica templates are reviewed while comparing these with traditional activated carbon production methods. Potential future directions for research and development activities are also discussed. Rice husk is a highly promising candidate for producing high-performance HPC materials.

层次化多孔碳（HPC）材料由于其发育良好的多尺度互联孔隙结构，在各种应用中表现出优异的性能。近几十年来，用天然生物质前体代替化石燃料前体合成高性能聚乙烯获得了相当大的关注。稻壳是一种全球丰富的农业废弃物，为高性能pc生产提供了一种可持续的、具有成本效益的前体。稻壳的结构成分和固有的二氧化硅含量为形成具有优越特性的分层孔结构提供了天然的自模板。本文综述了近年来以稻壳为原料制备高性能塑料的研究进展，并对合成技术进行了评述。此外，综述了近年来在活化方法和二氧化硅模板效果方面的研究进展，并与传统的活性炭生产方法进行了比较。研究和发展活动的潜在未来方向也进行了讨论。稻壳是一种极具潜力的高性能高性能材料。

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引用次数: 0

Optimizing Hybrid with Improved Resistance to Rice Blast and Superior Ratooning Ability 抗稻瘟病能力增强、再生力强的杂种优化

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-05-01 DOI: 10.1016/j.rsci.2025.04.015

Yi Liang , Zhaofeng Yi , Wen Zhuang , Teng Peng , Gui Xiao , Yunkai Jin , Qiyuan Tang , Jiaojun Xiong , Qiyun Deng , Bo Zhou , Xionglun Liu , Jun Wu

引用次数: 0

Function of R2R3-Type Myeloblastosis Transcription Factors in Plants r2r3型成髓细胞转录因子在植物中的作用

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-05-01 DOI: 10.1016/j.rsci.2025.01.007

Su Chen , Feilong Ma , Jiaoyang Chen , Man Qi , Qianshu Wei , Zhihuan Tao , Bo Sun

Myeloblastosis (MYB) transcription factors, particularly those in the R2R3 MYB subclass, are pivotal in plant growth, development, and environmental stress responses. As one of the largest transcription factor families in plants, the MYB family significantly regulates plant secondary metabolism, including the biosynthetic pathways for phenylpropanoids, which are crucial for stress resistance. This review presents a comprehensive overview of MYB transcription factor classification and their regulatory mechanisms in plant metabolism and stress responses. We discuss the roles of MYB transcription factors in biotic stress resistance, such as defense against pathogens and pests, and in abiotic stress tolerance, including responses to drought and salinity. Special attention is given to the interactions of R2R3 MYB with other transcription factors and co-repressors, focusing on how these synergistic or antagonistic relationships modulate physiological processes. The multifunctional role of R2R3 MYBs in stress responses positions them as promising targets for enhancing crop resilience through genetic breeding. Furthermore, this review highlights potential applications of MYB transcription factors in developing stress-resistant crops and their utility in plant resistant breeding programs.

髓母细胞病（MYB）转录因子，特别是R2R3 MYB亚类中的转录因子，在植物生长、发育和环境胁迫反应中起着关键作用。作为植物中最大的转录因子家族之一，MYB家族显著调控植物次生代谢，包括对抗逆性至关重要的苯丙素的生物合成途径。本文综述了MYB转录因子的分类及其在植物代谢和胁迫反应中的调控机制。我们讨论了MYB转录因子在生物抗逆性中的作用，如对病原体和害虫的防御，以及在非生物抗逆性中的作用，包括对干旱和盐度的反应。特别关注R2R3 MYB与其他转录因子和共抑制因子的相互作用，重点关注这些协同或拮抗关系如何调节生理过程。R2R3 myb在逆境反应中的多功能作用使其成为通过遗传育种提高作物抗逆性的有希望的靶点。此外，本文还综述了MYB转录因子在抗逆性作物开发中的潜在应用及其在植物抗性育种中的应用。

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引用次数: 0

Global Transcriptome Analysis of Rice Seedlings in Response to Extracellular ATP 水稻幼苗对细胞外ATP响应的转录组分析

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-05-01 DOI: 10.1016/j.rsci.2025.03.002

Chaemyeong Lim , Sae Hyun Lee , Haeun Lee , So-Yon Park , Kiyoon Kang , Hyeryung Yoon , Tae-Jin Yang , Gary Stacey , Nam-Chon Paek , Sung-Hwan Cho

Herbivorous insects and pathogens cause severe damage to rice tissues, affecting yield and grain quality. Damaged cells trigger downstream defense responses through various signals. Extracellular ATP (eATP), a signaling molecule released during mechanical cell damage, is considered a constitutive damage-associated molecular pattern (DAMP), which is crucial for initiating plant defense responses. Thus, understanding how rice plants cope with DAMPs such as eATP is essential. Here, we found that exogenous ATP affected rice growth and development, cell wall composition, chloroplast development, and cell death. Subsequent global transcriptome analysis revealed that several pathways were involved in the eATP response, including genes related to cell surface receptors, cell wall organization, chlorophyll biosynthesis, heat and temperature stimulation, epigenetic regulation, and reactive oxygen species metabolism. Cell surface receptors, including members of the lectin receptor-like kinases (LecRKs), were found to participate in the eATP response. We further investigated ATP-induced genes in T-DNA activation mutants of OsLecRKs, demonstrating their involvement in eATP signaling in rice. This study confirms a DAMP-mediated transcriptional response in plants and provides novel candidates for advancing resistant rice breeding against insect herbivores and pathogens.

草食性昆虫和病原体对水稻组织造成严重损害，影响产量和稻米品质。受损细胞通过各种信号触发下游防御反应。细胞外ATP （eATP）是细胞机械损伤过程中释放的一种信号分子，被认为是一种本构性损伤相关分子模式（DAMP），在启动植物防御反应中起着至关重要的作用。因此，了解水稻植物如何应对像eATP这样的damp是至关重要的。在这里，我们发现外源ATP影响水稻的生长发育、细胞壁组成、叶绿体发育和细胞死亡。随后的全球转录组分析显示，eATP反应涉及多种途径，包括与细胞表面受体、细胞壁组织、叶绿素生物合成、热和温度刺激、表观遗传调控和活性氧代谢相关的基因。包括凝集素受体样激酶（LecRKs）成员在内的细胞表面受体被发现参与了eATP反应。我们进一步研究了OsLecRKs T-DNA激活突变体中的atp诱导基因，证实了它们参与水稻eATP信号传导。该研究证实了植物中damp介导的转录反应，为推进水稻抗虫、抗草食动物和抗病原体育种提供了新的候选材料。

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引用次数: 0

Generational Genetic Mechanism of Space Mutagenesis in Rice Based on Multi-Omics 基于多组学的水稻空间诱变世代遗传机制研究

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2025-05-01 DOI: 10.1016/j.rsci.2025.03.001

Zeng Deyong , Cui Jie , Yin Yishu , Dai Cuihong , Yu Wencheng , Zhao Haitian , Guan Shuanghong , Cheng Dayou , Sun Yeqing , Lu Weihong

Intergenerational inheritance of stress memory plays a crucial role in plant adaptation to environmental changes, particularly in the context of spaceflight, where plants may serve as a food source for humans on long-duration missions. However, the intergenerational genetic effects of spaceflight-induced stress memory in plants remain unclear. In this study, we assessed the cross-generational genetic effects of spaceflight stress memory using the rice mutant B10, identified during the SJ-10 return satellite mission. Our results showed that the oxidative stress effects induced by spaceflight persisted until the M5 generation in rice. We found that the rice genome remained unstable post-spaceflight, leading to alterations in genome methylation levels. Additionally, we observed significant changes in the methylation levels of transposons, suggesting their involvement in the intergenerational inheritance of spaceflight-induced stress memory. Furthermore, we identified thousands of differentially expressed genes (DEGs) and differentially alternatively spliced (DAS) genes induced by spaceflight stress memory across multiple rice generations. Notably, differentially methylated cytosines were more abundant in non-expressed genes than in DEGs or DAS genes. A substantial number of DEGs and DASs related to oxidative stress were identified, primarily involved in the generation and scavenging of reactive oxygen species. This study also presented report on the response of alternative splicing events in rice to spaceflight stress. Moreover, our findings revealed that genome methylation was associated with gene expression but not with DAS. In conclusion, our study provides comprehensive insights into the intergenerational inheritance of spaceflight-induced stress in rice and may contribute to uncovering novel mechanisms of oxidative stress-induced genomic instability and epigenetic regulation in plant stress inheritance.

压力记忆的代际遗传在植物适应环境变化中起着至关重要的作用，特别是在太空飞行的背景下，植物可能作为长期任务中人类的食物来源。然而，太空飞行诱导的植物应激记忆的代际遗传效应仍不清楚。在这项研究中，我们利用SJ-10返回卫星任务中鉴定的水稻突变体B10评估了航天胁迫记忆的跨代遗传效应。结果表明，航天诱导的氧化应激效应一直持续到水稻的M5代。我们发现，水稻基因组在太空飞行后仍然不稳定，导致基因组甲基化水平的改变。此外，我们观察到转座子甲基化水平的显著变化，表明它们参与了太空飞行诱导的应激记忆的代际遗传。此外，我们在多个水稻世代中鉴定了数千个由航天胁迫记忆诱导的差异表达基因（DEGs）和差异选择性剪接（DAS）基因。值得注意的是，差异甲基化的胞嘧啶在非表达基因中比在DEGs或DAS基因中更丰富。大量与氧化应激相关的DEGs和DASs被鉴定出来，主要参与活性氧的产生和清除。本研究还报道了水稻选择性剪接事件对航天胁迫的响应。此外，我们的研究结果表明，基因组甲基化与基因表达相关，而与DAS无关。综上所述，本研究为水稻航天胁迫的代际遗传提供了全面的见解，并可能有助于揭示氧化应激诱导的基因组不稳定性和植物胁迫遗传中的表观遗传调控的新机制。

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引用次数: 0

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Pub Date : 2025-05-01 DOI: 10.1016/j.rsci.2025.01.003

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IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

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引用次数: 0

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