Rice Science最新文献

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Synergy in Rice Immunity: Exploring Strategies of Coordinated Disease Defense Through Receptor-Like Kinases and Receptor- Like Cytoplasmic Kinases

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-11-01 DOI: 10.1016/j.rsci.2024.07.002

Mengtian Pei , Yingying Cao , Xuze Xie , Ying Cao , Jia Chen , Xi Zhang , Zonghua Wang , Guodong Lu , Shenghang Zhang

Receptor-like kinases (RLKs) and receptor-like cytoplasmic kinases (RLCKs) play an indispensable role in the perception and transmission of extracellular signals in plants. In rice, these kinases actively participate in immune responses against a variety of pathogens, including fungi, bacteria, and viruses. However, research on the specific response mechanisms and the spectrum of different kinase activities against various pathogens remains insufficient. This review provides an in-depth and comprehensive overview of the types and functions of RLKs and RLCKs involved in disease resistance, emphasizing the central role of certain RLKs and RLCKs in the plant immune system. These kinases can recognize specific molecular patterns of pathogens and rapidly initiate an immune response in rice. Furthermore, the activity and functional regulation of these key kinases are tightly controlled by various post-translational modifications, such as phosphorylation and ubiquitination. This meticulous regulation ensures that the rice immune system’s response is both precise and timely, effectively balancing the intensity of the immune response and preventing potential issues caused by either hyperactivity or insufficiency. By synthesizing current research findings, this review not only broadens our understanding of the role of RLKs and RLCKs in plant immunity but also provides new perspectives and strategies for future research on disease resistance breeding in rice. Future studies are expected to delve deeper into the signaling networks and regulatory mechanisms of these kinases, exploring their potential in agricultural production to develop rice varieties with enhanced disease resistance.

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引用次数: 0

Efficient Breeding of Early-Maturing Rice Cultivar by Editing Hd6 via CRISPR/Cas9 通过 CRISPR/Cas9 编辑 Hd6 高效培育早熟水稻品种

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-11-01 DOI: 10.1016/j.rsci.2024.06.007

Chen Zhihui , Tao Yajun , Xu Yang , Wu Jingjing , Wang Fangquan , Li Wenqi , Jiang Yanjie , Fan Fangjun , Li Xia , Zhu Jianping , Zhu Qian-Hao , Yang Jie

引用次数: 0

Compound Microbial Agent Improves Soil Redox Status to Reduce Methane Emissions from Paddy Fields 复合微生物制剂改善土壤氧化还原状态，减少稻田甲烷排放

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-11-01 DOI: 10.1016/j.rsci.2024.05.002

Tao Yi , Xiao Deshun , Ye Chang , Liu Kancheng , Tang Xinxin , Ma Hengyu , Chu Guang , Yu Kai , Xu Chunmei , Wang Danying

Paddy fields are considered a major source of methane (CH₄) emissions. Aerobic irrigation methods have proven to be efficacious in mitigating CH₄ emissions in paddy cultivation. The promising role of compound microbial agents in refining the rhizospheric ecosystem suggests their potential as novel agents in reducing CH₄ emissions from paddy fields. To explore a new method of using compound microbial agents to reduce CH₄ emissions, we conducted pot and field experiments over the period of 2022‒2023. We measured CH₄ flux, the redox potential (Eh) of the soil, the concentration of dissolved oxygen (DO) in the floodwater, and the gene abundance of both methanogens (mcrA) and methanotrophs (pmoA). The results showed that the application of the compound microbial agent led to a significant increase in the DO levels within the floodwater and an increase of 9.26% to 35.01% in the Eh of the tillage soil. Furthermore, the abundance of pmoA increased by 31.20%, while the mcrA/pmoA ratio decreased by 25.96% at the maximum tillering stage. Applying 45−75 kg/hm² of the compound microbial agent before transplanting resulted in a reduction of cumulative CH₄ emissions from paddy fields by 17.49% in single- cropped rice and 43.54% to 50.27% in double-cropped late rice during the tillering stage. Correlation analysis indicated that CH₄ flux was significantly negatively correlated with pmoA gene abundance and soil Eh, and positively related to the mcrA/pmoA ratio. Additionally, soil Eh was significantly positively correlated with pmoA gene abundance, suggesting that paddy soil Eh indirectly affected CH₄ flux by influencing the pmoA gene abundance. In conclusion, the pre-planting application of the compound microbial agent at a rate of 45‒75 kg/hm² can enhance the Eh in the rhizosphere and increase the abundance of the pmoA gene, thereby reducing CH₄ emissions from paddy fields during the tillering stage of rice growth.

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引用次数: 0

Progress on Molecular Mechanism of Heat Tolerance in Rice

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-11-01 DOI: 10.1016/j.rsci.2024.07.001

Fu Yiwei , Wu Jiayelu , Wu Mingming , Ye Shenghai , Zhai Rongrong , Ye Jing , Zhu Guofu , Yu Faming , Lu Yanting , Zhang Xiaoming

Rice (Oryza sativa L.) is a major food crop in China, and its high and stable yield is crucial for ensuring food security in the country. However, over the past few years, extreme weather events induced by global climate change have impacted rice growth. For example, the effects of heat stress on rice quality and yield have been significant. Therefore, it is fundamental to conduct in-depth research on the heat-tolerance mechanisms of rice and to cultivate superior new thermotolerant rice varieties. This review summarizes the adverse effects of high temperatures on rice growth at various stages, the heat-tolerance mechanisms in rice, and the heat-tolerance genes and QTLs that have been identified in recent years. We also discuss strategies to enhance the heat tolerance of rice, offering new insights for rice breeding research.

引用次数: 0

Higher Grain-Filling Rate in Inferior Spikelets of Tolerant Rice Genotype Offset Grain Yield Loss under Post-Anthesis High Night Temperatures 耐寒水稻基因型下位小穗的较高籽粒充实率抵消了开花后高夜温条件下的籽粒产量损失

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.06.003

Nitin Sharma , Bhupinder Singh , Subbaiyan Gopala Krishnan , Haritha Bollinedi , Pranab Kumar Mandal , Milan Kumar Lal , Prakash Kumar Jha , P.V.Vara Prasad , Anjali Anand

Increased nighttime respiratory losses decrease the amount of photoassimilates available for plant growth and yield. We hypothesized that the increased respiratory carbon loss under high night temperatures (HNT) could be compensated for by increased photosynthesis during the day following HNT exposure. Two rice genotypes, Vandana (HNT-sensitive) and Nagina 22 (HNT-tolerant), were exposed to HNT (4 °C above the control) from flowering to physiological maturity. They were assessed for alterations in the carbon balance of the source (flag leaf) and its subsequent impact on grain filling dynamics and the quality of spatially differentiated sinks (superior and inferior spikelets). Both genotypes exhibited significantly higher night respiration rates. However, only Nagina 22 compensated for the high respiration rates with an increased photosynthetic rate, resulting in a steady production of total dry matter under HNT. Nagina 22 also recorded a higher grain-filling rate, particularly at 5 and 10 d after flowering, with 1.5- and 4.0-fold increases in the translocation of ¹⁴C sugars to the superior and inferior spikelets, respectively. The ratio of photosynthetic rate to respiratory rate on a leaf area basis was negatively correlated with spikelet sterility, resulting in a higher filled spikelet number and grain weight per plant, particularly for inferior grains in Nagina 22. Grain quality parameters such as head rice recovery, high-density grains, and gelatinization temperature were maintained in Nagina 22. An increase in the rheological properties of rice flour starch in Nagina 22 under HNT indicated the stability of starch and its ability to reorganize during the cooling process of product formation. Thus, our study showed that sink adjustments between superior and inferior spikelets favored the growth of inferior spikelets, which helped to offset the reduction in grain weight under HNT in the tolerant genotype Nagina 22.

夜间呼吸损失的增加会降低植物生长和产量所需的光同化物的数量。我们假设，在夜间高温（HNT）条件下，呼吸碳损失的增加可以通过白天光合作用的增加来补偿。两种水稻基因型 Vandana（对 HNT 敏感）和 Nagina 22（对 HNT 耐受）从开花期到生理成熟期都暴露在 HNT（比对照温度高 4 °C）下。对它们进行了评估，以确定源（旗叶）碳平衡的变化及其随后对谷粒灌浆动态和空间上有差异的汇（上穗和下穗）质量的影响。两种基因型的夜间呼吸速率都明显较高。然而，只有 Nagina 22 通过提高光合速率来补偿高呼吸速率，从而在 HNT 条件下保持稳定的总干物质产量。Nagina 22 的籽粒充实率也较高，特别是在花后 5 d 和 10 d，14C 糖分向上部和下部小穗的转移分别增加了 1.5 倍和 4.0 倍。按叶面积计算的光合速率与呼吸速率之比与小穗不育率呈负相关，从而导致每株的灌浆穗数和粒重增加，尤其是长稻 22 的劣质穗。纳吉纳 22 的谷粒质量参数，如头米回收率、高密度谷粒和糊化温度都保持不变。在 HNT 条件下，Nagina 22 中米粉淀粉流变特性的增加表明淀粉的稳定性及其在产品形成的冷却过程中重组的能力。因此，我们的研究表明，上等穗和下等穗之间的穗沉调整有利于下等穗的生长，这有助于抵消耐逆基因型 Nagina 22 在 HNT 条件下的粒重减少。

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引用次数: 0

Improving Semi-Dried Brown Rice Noodle Quality via Mixed Fermentation of Lactobacillus and Yeast 通过乳酸菌和酵母的混合发酵提高半干糙米面的质量

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.06.005

引用次数: 0

RISE Method Based on Rare Allele Infusion and Sanger Sequencing Estimation: A Simple, Cheap, and Efficient Method for Detecting Transgene Copy Number in Rice 基于稀有等位基因注入和 Sanger 测序估算的 RISE 方法：一种检测水稻转基因拷贝数的简单、廉价而高效的方法

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.05.001

引用次数: 0

Rice Cultivation under Film Mulching Can Improve Soil Environment and Be Beneficial for Rice Production in China 薄膜覆盖栽培水稻可改善土壤环境，有利于中国水稻生产

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.06.009

Youliang Zhang, Kaican Zhu, Yongqi Tang, Shaoyuan Feng

Rice cultivation under film mulching is an integrated management technology that can conserve water, increase soil temperature, improve yield, and enhance water and nitrogen use efficiencies. Despite these advantages, the system does have its drawbacks, such as soil organic matter reduction and microplastic pollution, which impede the widespread adoption of film mulching cultivation in China. Nonetheless, the advent of degradable film, controlled-release fertilizer, organic fertilizer, and film mulching machinery is promoting the development of rice film mulching cultivation. This review outlines the impact of rice cultivation under film mulching on soil moisture, soil temperature, soil fertility, greenhouse gas emissions, weed control, and disease and pest management. It also elucidates the mechanism of changes in rice growth, yield and quality, water use efficiency, and nitrogen use efficiency. This paper incorporates a review of published research articles and discusses some uncertainties and shortcomings associated with rice cultivation under film mulching. Consequently, prospective research directions for the technology of rice film mulching cultivation are outlined, and recommendations for future research into rice cultivation under film mulching are proposed.

地膜覆盖水稻栽培是一项综合管理技术，可以节水、提高土壤温度、提高产量、提高水和氮的利用效率。尽管有这些优点，但该系统也有其缺点，如土壤有机质减少和微塑料污染，这阻碍了地膜覆盖栽培在中国的广泛应用。不过，可降解薄膜、控释肥、有机肥和覆膜机械的出现正在推动水稻覆膜栽培的发展。本综述概述了水稻覆膜栽培对土壤水分、土壤温度、土壤肥力、温室气体排放、杂草控制和病虫害防治的影响。它还阐明了水稻生长、产量和质量、水利用效率和氮利用效率的变化机理。本文对已发表的研究文章进行了综述，并讨论了与薄膜覆盖水稻栽培相关的一些不确定性和不足之处。因此，本文概述了水稻膜下地膜覆盖栽培技术的前瞻性研究方向，并提出了未来膜下地膜覆盖水稻栽培研究的建议。

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引用次数: 0

Bulked Segregant RNA-Seq Analysis of Pollinated Pistils Reveals Genes Influencing Spikelet Fertility in Rice 对授粉雌蕊的大块分离RNA-Seq分析揭示了影响水稻小穗生育力的基因

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.06.001

Kanokwan Kaewmungkun , Keasinee Tongmark , Sriprapai Chakhonkaen , Numphet Sangarwut , Theerachai Thanananta , Amorntip Muangprom

Prezygotic isolation is important for successful fertilization in rice, significantly affecting yield. This study focused on F_5:6 generation plants derived from inter-subspecific crosses (Nipponbare × KDML105) with low (LS) and high seed-setting rates (HS), in which normal pollen fertility was observed. However, LS plants showed a reduced number of pollen grains adhering to the stigma and fewer pollen tubes reaching the ovules at 4‒5 h post-pollination, compared with HS plants. Bulked segregant RNA-Seq analysis of pollinated pistils from the HS and LS groups revealed 249 and 473 differentially expressed genes (DEGs), respectively. Kyoto Encyclopedia of Genes and Genomes analysis of the HS and LS- specific DEGs indicated enrichment in metabolic pathways, pentose and glucuronate interconversions, and flavonoid biosynthesis. Several of these DEGs exhibited co-expression with pollen development genes and formed extensive clusters of co-expression networks. Compared with LS pistils, enzyme genes controlling pectin degradation, such as OsPME35 and OsPLL9, showed similar expression patterns, with higher levels in HS pistils pre-pollination. Os02g0467600, similar to cinnamate 4-hydroxylase gene (CYP73), involved in flavonoid biosynthesis, displayed higher expression in HS pistils post-pollination. Our findings suggest that OsPME35, OsPLL9, and Os02g0467600 contribute to prezygotic isolation by potentially modifying the stigma cell wall (OsPME35 and OsPLL9) and controlling later processes such as pollen-stigma adhesion (Os02g0467600) genes. Furthermore, several DEGs specific to HS and LS were co-localized with QTLs and functional genes associated with spikelet fertility. These findings provide valuable insights for further research on rice spikelet fertility, ultimately contributing to the development of high-yielding rice varieties.

祖先隔离对水稻成功受精非常重要，会显著影响产量。本研究的重点是由低结实率（LS）和高结实率（HS）的亚种间杂交（Nipponbare × KDML105）产生的 F5:6 代植株。然而，与 HS 植物相比，LS 植物在授粉后 4-5 小时粘附在柱头上的花粉粒数量减少，到达胚珠的花粉管数量也减少。对HS组和LS组授粉雌蕊的大量分离RNA-Seq分析分别发现了249个和473个差异表达基因（DEGs）。京都基因和基因组百科全书》对 HS 和 LS 特异性 DEGs 的分析表明，这些 DEGs 在代谢途径、戊糖和葡萄糖醛酸的相互转化以及类黄酮的生物合成方面具有丰富的表达。其中一些 DEGs 与花粉发育基因共表达，并形成了广泛的共表达网络群。与LS雌蕊相比，OsPME35和OsPLL9等控制果胶降解的酶基因表现出相似的表达模式，在HS雌蕊授粉前的表达水平更高。Os02g0467600 与肉桂酸 4-羟化酶基因（CYP73）类似，参与黄酮类化合物的生物合成，在 HS 授粉后雌蕊中的表达量较高。我们的研究结果表明，OsPME35、OsPLL9和Os02g0467600可能通过改变柱头细胞壁（OsPME35和OsPLL9）和控制后期过程（如花粉-柱头粘附（Os02g0467600）基因）来促进祖先分离。此外，一些特异于 HS 和 LS 的 DEGs 与小穗生育力相关的 QTL 和功能基因共定位。这些发现为进一步研究水稻小穗生育力提供了有价值的见解，最终有助于培育高产水稻品种。

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Biochar Decreases Soil Cadmium (Cd) Availability and Regulates Expression Levels of Cd Uptake/Transport-Related Genes to Reduce Cd Translocation in Rice 生物炭降低土壤中镉的供应量并调节镉吸收/转运相关基因的表达水平，从而减少水稻中镉的转运

IF 5.6 2区农林科学 Q1 AGRONOMY

Rice Science

Pub Date : 2024-09-01 DOI: 10.1016/j.rsci.2024.04.004

引用次数: 0

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