Infection prevention is critical for improving safety and care quality, reducing mortality, and enhancing the quality of life in nursing homes for older adults. These establishments often lack standardized guidelines, and have inconsistent protocols. This study evaluated the Evidence-Based Guidelines for Nursing Home Infection Control in South Korea (ENIK) by examining healthcare workers’ (HCWs) experiences. Five focus groups, involving 28 HCWs in total, were analyzed using qualitative content analysis and the Systems Engineering Initiative for Patient Safety framework. Participants reported improved infection prevention and control after ENIK implementation. Key motivators included knowledge acquisition, retraining, and positive experiences. Challenges involved staff turnover and external control factors. Clear job roles enhanced efficiency and confidence. Supportive resources (hand hygiene tools, infection control specialists, checklists, and visual aids) and organizational measures (rewards, teamwork, and control systems) were essential in streamlining infection management. ENIK fostered knowledge, clear roles, resource availability, and systematic management, enhancing infection control and care quality.
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