R. Frassini, D. Steffens, S. Moura, C. Aguzzoli, A. P. Martins, P. Colepicolo, M. Fujii, N. Yokoya, C.M.P. Pereira, Ana Cláudia Phillipus, M. Falkenberg, J. P. Henriques, M. Roesch-Ely
{"title":"<i>Desmarestia anceps</i> Montagne (Phaeophyceae) against Colorectal Cancer Cells: Cytotoxic Activity and Proapoptotic Effects","authors":"R. Frassini, D. Steffens, S. Moura, C. Aguzzoli, A. P. Martins, P. Colepicolo, M. Fujii, N. Yokoya, C.M.P. Pereira, Ana Cláudia Phillipus, M. Falkenberg, J. P. Henriques, M. Roesch-Ely","doi":"10.4236/abc.2022.126019","DOIUrl":"https://doi.org/10.4236/abc.2022.126019","url":null,"abstract":"","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70475900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Phytochemical Analysis and Bioactivity Screening of Primary and Secondary Metabolic Products of Medicinal Plants in the Valleys of Medina Region Saudi Arabia","authors":"Safa Alsaedi, G. Aljeddani","doi":"10.4236/abc.2022.124009","DOIUrl":"https://doi.org/10.4236/abc.2022.124009","url":null,"abstract":"","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70476090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The present work describes the synthesis of a novel heterocyclic azo dye by general diazonization of 2-amino-4,5-dimethylthiazole followed by the diazo- coupling of the resulting diazonium ion with 5-methyl-2-(propan-2-yl)phenol to obtain ligand L. This was characterized using Fourier-transformed infrared and electronic spectrophotometry. Ligand L was further coordinated with five metal ions, M:L, 1:2 [M = Cu(II), Mn(II), Zn(II), Ni(II) and Co(II)]. The coordination compounds obtained were characterized by electronic, IR spectrophotometry, magnetic susceptibility and percentage metal analyses. The results obtained suggested that a thiazoylazo dye was obtained as ligand L. It was proposed that two molecules of the solvent coordinated to the metal ion in addition with the ligands to give an octahedral geometry for copper(II), manganese(II) and nickel(II) complexes. On the other hand, square planar geometry was suggested for zinc(II) and cobalt(II) complexes. The anti-infla- mmatory activity of the ligand and coordination compounds was evaluated using four in vitro-based assays viz: xanthine oxidase and lipoxygenase inhibition assay, membrane stability and protein denaturation assay. The synthesized compounds generally exhibited good anti-inflammatory activity in all the assays carried out. However, the reference standards, in this instance, were more effective in the case of xanthine oxidase, lipoxygenase and protein denaturation inhibitory assays. For the membrane stability study, the coordination compounds and ligand L elicited more potent anti-inflammatory activity than the standard drug.
{"title":"Syntheses, Characterization and Biological Activity of Novel Thiazoylazo Dye and Its Coordination Compounds","authors":"T. Aiyelabola","doi":"10.4236/abc.2021.115013","DOIUrl":"https://doi.org/10.4236/abc.2021.115013","url":null,"abstract":"The present work describes the synthesis of a novel heterocyclic azo dye \u0000by general diazonization of \u00002-amino-4,5-dimethylthiazole followed by the diazo- coupling \u0000of the resulting diazonium ion with 5-methyl-2-(propan-2-yl)phenol to obtain ligand L. This was \u0000characterized using Fourier-transformed infrared and electronic \u0000spectrophotometry. Ligand L was further coordinated with five metal ions, M:L, 1:2 [M = Cu(II), Mn(II), Zn(II), Ni(II) and Co(II)]. The coordination \u0000compounds obtained were characterized by electronic, IR spectrophotometry, \u0000magnetic susceptibility and percentage metal analyses. The results obtained suggested \u0000that a thiazoylazo dye was obtained as ligand L. It was proposed that \u0000two molecules of the solvent coordinated to the metal ion in addition with the \u0000ligands to give an octahedral geometry for copper(II), manganese(II) and \u0000nickel(II) complexes. On the other hand, square planar geometry was suggested for zinc(II) and cobalt(II) complexes. The anti-infla- mmatory \u0000activity of the ligand and coordination compounds was evaluated using four in vitro-based \u0000assays viz: xanthine oxidase and \u0000lipoxygenase inhibition assay, membrane stability and protein denaturation \u0000assay. The synthesized compounds generally exhibited good anti-inflammatory \u0000activity in all the assays carried out. \u0000However, the reference standards, in this instance, were more effective \u0000in the case of xanthine oxidase, lipoxygenase and protein denaturation \u0000inhibitory assays. For the membrane stability study, the coordination compounds and ligand L elicited \u0000more potent anti-inflammatory activity than the standard drug.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48852083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
With the increasing demand for imaging quality, many scientists constantly explore new imaging instruments to meet the requirements. The total internal reflection fluorescence microscopy has some incomparable superiorities, so it has become one of the research hotspots in recent years. It can show great performance in single-molecule imaging because it has unique imaging principles. This apparatus is used mainly in two fields, biomolecule research and clinical medicine. To know this instrument’s function, the summary of applications in these two parts was given in this article. Now, scientists who have been focusing on this apparatus try to make this microscope combined with other late-model precise instruments that probe some unknown interaction mechanism of action. The TIRFM will show extraordinary talents in many aspects, and it will become a powerful tool for people to explore the mysteries of life.
{"title":"Application of TIRFM in Biomolecule Research and Clinical Medicine","authors":"Ye-Qin Zhang","doi":"10.4236/abc.2021.115014","DOIUrl":"https://doi.org/10.4236/abc.2021.115014","url":null,"abstract":"With the increasing demand for imaging quality, many scientists \u0000constantly explore new imaging instruments to meet the requirements. The total \u0000internal reflection fluorescence microscopy has some incomparable superiorities, so it has become one of the research hotspots in \u0000recent years. It can show great performance in single-molecule imaging because \u0000it has unique imaging principles. This apparatus is used mainly in two fields, \u0000biomolecule research and clinical medicine. To know this instrument’s function, \u0000the summary of applications in these two parts was given in this article. Now, scientists who have been focusing on this apparatus try to make this microscope combined with other late-model \u0000precise instruments that probe some unknown interaction mechanism of action. The TIRFM will show extraordinary talents in many aspects, and it will become a powerful tool for people to explore the \u0000mysteries of life.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41949758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
J. Mpetga, A. R. N. Donfack, J. Tamokou, Iréne Chinda Kengne, P. Tane, Xiao-jiang Hao, Mathieu Tene
Chemical investigation of the MeOH extract from the leaves of C. glauca yielded nine known triterpenoids (1-9) belonging to the cycloartane and friedelane series. Two of these compounds namely glaucartanoic acid A (1) and 3β,21β-dihydroxy-30-nor-(D:A)-friedoolean-20(29)-en-27-oic acid (5) were subjected to chemical derivatizations and afforded five new derivatives: diacetylglaucartanoic acid A (1a), 24-acetylglaucartanoic acid A (1b), glaucartanoic acid A methyl ester (1c), 24-methoxyglaucartanoic acid A methyl ester (1d), and 3β,21β-diacetoxy-30-nor-(D:A)-friedoolean-20(29)-en-27-oic acid (5a). Their structures were assigned based on their NMR and MS data and by comparison with literature values. The MeOH extract, isolated compounds and some new semi-synthetic derivatives were subjected to in vitro antimicrobial assays against a panel of pathogenic microorganisms, including Gram-positive and Gram-negative bacteria, and fungi using broth microdilution method. The MeOH extract displayed activity towards all the tested pathogenic bacterial and fungal strains with good activity (MIC against Staphylococcus aureus ATCC25923 and Shigella flexneri SDINT. Compounds 3 and 5 showed the most potent antimicrobial effect.
{"title":"Triterpenoids and Semisynthetic Derivatives with Antimicrobial Activities from the Leaves of Caloncoba glauca (Flacourtiaceae)","authors":"J. Mpetga, A. R. N. Donfack, J. Tamokou, Iréne Chinda Kengne, P. Tane, Xiao-jiang Hao, Mathieu Tene","doi":"10.4236/abc.2021.114011","DOIUrl":"https://doi.org/10.4236/abc.2021.114011","url":null,"abstract":"Chemical investigation of the MeOH extract from the \u0000leaves of C. glauca yielded nine known triterpenoids (1-9) \u0000belonging to the cycloartane and friedelane series. Two of these \u0000compounds namely glaucartanoic acid A (1) and 3β,21β-dihydroxy-30-nor-(D:A)-friedoolean-20(29)-en-27-oic \u0000acid (5) were subjected to chemical derivatizations and afforded five \u0000new derivatives: diacetylglaucartanoic acid A (1a), \u000024-acetylglaucartanoic acid A (1b), glaucartanoic acid A methyl ester (1c), \u000024-methoxyglaucartanoic acid A methyl ester \u0000(1d), and 3β,21β-diacetoxy-30-nor-(D:A)-friedoolean-20(29)-en-27-oic acid (5a). Their structures were assigned based on their NMR and MS data \u0000and by comparison with literature values. \u0000The MeOH extract, isolated compounds and some new semi-synthetic \u0000derivatives were subjected to in vitro antimicrobial assays \u0000against a panel of pathogenic microorganisms, including Gram-positive \u0000and Gram-negative bacteria, and fungi using broth microdilution method. The \u0000MeOH extract displayed activity towards all the tested pathogenic bacterial and fungal strains with good \u0000activity (MIC against Staphylococcus aureus ATCC25923 and Shigella flexneri SDINT. Compounds 3 and 5 showed the most potent antimicrobial effect.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42607891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ablo Evrard, Coulibali Sioménan, Camara Tchambaga Etienne, Touré Daouda, C. Souleymane, Sissouma Drissa, A. Ané
A series of novel substituted benzimidazole (7a - n) derivatives were synthesized and characterized by 1H, 13C Nuclear Magnetic Resonance (NMR) spectra and High Resolution Mass Spectrometry (HRMS). The substitution was done in position -1 and -2 by appropriate groups. These compounds are obtained by N-alkylation reaction with thiomethyl-1H-benzimidazole intermediates (5a - g). Design of intermediates (5a - g) was made by condensation reaction between 2-methylbenzimidazole thiourunium salt (3) and functionalized halides (4) in the presence of sodium hydroxide (NaOH). Among the twenty-one compounds synthesized, ten were evaluated for their antimicrobial activity on three bacterial strains namely: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Only E. coli ATTC 25922 was susceptible to the synthesized derivatives 5g, 7f and 7h with a significant antibacterial activity (CMI is between 250 and 500 μg/mL).
{"title":"Design, Synthesis and in Vitro Antibacterial Activity of 2-thiomethyl-benzimidazole Derivatives","authors":"Ablo Evrard, Coulibali Sioménan, Camara Tchambaga Etienne, Touré Daouda, C. Souleymane, Sissouma Drissa, A. Ané","doi":"10.4236/abc.2021.114012","DOIUrl":"https://doi.org/10.4236/abc.2021.114012","url":null,"abstract":"A series \u0000of novel substituted benzimidazole (7a - n) derivatives were synthesized and characterized by 1H, 13C Nuclear \u0000Magnetic Resonance (NMR) spectra and High Resolution Mass Spectrometry (HRMS). \u0000The substitution was done in position -1 and -2 by appropriate groups. These compounds are \u0000obtained by N-alkylation reaction with thiomethyl-1H-benzimidazole \u0000intermediates (5a - g). Design of intermediates (5a - g) was made by condensation reaction between 2-methylbenzimidazole \u0000thiourunium salt (3) and functionalized halides (4) in the \u0000presence of sodium hydroxide (NaOH). Among the twenty-one compounds synthesized, ten were evaluated for their antimicrobial \u0000activity on three bacterial strains namely: Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 25923 and Pseudomonas aeruginosa ATCC 27853. Only E. coli ATTC 25922 was \u0000susceptible to the synthesized derivatives 5g, 7f and 7h with a significant antibacterial activity (CMI is between 250 and 500 μg/mL).","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49509471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Non-template [2 + 2] condensation of benzene-1,4-dicarboxaldehyde with two diamines: ethane-1,2-diamine and propane-1,2-diamine was carried out to give Ligands 1 and 2. Additionally, template [1 + 2] condensation of benzene-1,4-dicarboxaldehyde with ethane-1,2-diamine and propane-1,2-diamine were also carried out to give Ligands 3 and 4. These were characterized using 1H and 13C NMR, UV-Vis and infra-red spectroscopy and mass spectrometry. Coordination compounds of each ligand were further synthesized using Ni(II) and Cu(II) ions. They were characterized by Uv-vis, infrared spectroscopy, mass spectrometry, magnetic susceptibility and energy dispersion X-ray spectroscopy EDX. The ligands and complexes were further analyzed for their antimicrobial activities and extraction efficiency. The results obtained suggested that tetraaza macrocyclic and linear compounds were obtained for [2 + 2] and [1 + 2] condensation reactions respectively. Coordination compounds of the macrocyclic ligand yielded octahedral geometry for Ni(II) and Cu(II) complexes of Ligand 1 and square planar geometry for Ni(II) and Cu(II) complexes of Ligand 2. On the other hand square planar geometry was proposed for coordination compounds of Ligand 3 and 4 exception for the Ni(II) complex of Ligand 3. All ligands coordinated to the metal ion in a tetradentate fashion. In some cases chelation enhanced the antimicrobial activity of some of the ligands. The results further showed that Ligands 1-4 effectively extracted cadmium(II). zinc(II) and lead(II) ions in solution.
{"title":"Syntheses, Characterization, Antimicrobial Activity and Extraction Studies of Tetraaza Macrocyclic/Linear Schiff Bases Derived from Benzene-1,4-Dicarboxaldehyde and Their Coordination Compounds","authors":"T. Aiyelabola, J. Jordaan, D. Otto, E. Akinkunmi","doi":"10.4236/ABC.2021.113007","DOIUrl":"https://doi.org/10.4236/ABC.2021.113007","url":null,"abstract":"Non-template [2 + 2] condensation of benzene-1,4-dicarboxaldehyde \u0000with two diamines: ethane-1,2-diamine and propane-1,2-diamine was carried out to give Ligands 1 and 2. Additionally, \u0000template [1 + 2] condensation of benzene-1,4-dicarboxaldehyde \u0000with ethane-1,2-diamine and propane-1,2-diamine were also carried out to \u0000give Ligands 3 and 4. These were characterized using 1H \u0000and 13C NMR, UV-Vis and infra-red spectroscopy and mass \u0000spectrometry. Coordination compounds of each ligand were further synthesized \u0000using Ni(II) and Cu(II) ions. They were characterized by Uv-vis, infrared spectroscopy, mass spectrometry, \u0000magnetic susceptibility and energy dispersion X-ray spectroscopy EDX. The \u0000ligands and complexes were further analyzed for their antimicrobial activities and extraction \u0000efficiency. The results obtained suggested that tetraaza macrocyclic and linear \u0000compounds were obtained for [2 + 2] and [1 + 2] condensation reactions respectively. \u0000Coordination compounds of the macrocyclic ligand yielded octahedral geometry \u0000for Ni(II) and Cu(II) complexes of Ligand 1 and square planar geometry \u0000for Ni(II) and Cu(II) complexes of Ligand 2. On the other hand square \u0000planar geometry was proposed for coordination compounds of Ligand 3 and 4 exception for the Ni(II) complex of Ligand 3. All ligands coordinated to the \u0000metal ion in a tetradentate fashion. In some cases chelation enhanced the \u0000antimicrobial activity of some of the ligands. The results further showed that \u0000Ligands 1-4 effectively extracted cadmium(II). zinc(II) and \u0000lead(II) ions in solution.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42508690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Coordination compounds of 3-hydroxy-2-methyl-4H-pyran-4-one with iron(III), cobat(III) and chromium(III) were synthesized with M:L (1:2). Mixed ligand coordination compounds of 3-hydroxy-2-methyl-4H-pyran-4-one and 1,2-diaminocyclohexane using the same metal ions were also synthesized M:L1:L2 (1:1:1) where L1 is 3-hydroxy-2-methyl-4H-pyran-4-one and L2 is 1,2-diaminocyclohexane. The coordination compounds obtained were characterized using electronic and infrared spectral analyses, magnetic susceptibility and percentage metal analysis. They were also evaluated for their cytotoxic and antioxidant activities. The result obtained suggested that octahedral geometry was obtained for all the compounds, as a result of additional two molecules of the solvent coordinated to the metal ions. Both the primary and secondary ligands coordinated in a bidentate fashion. The synthesized compounds exhibited moderate cytotoxicity, although none was as active as the standard. The cobalt(III) mixed ligand complex elicited the highest activity. The synthesized compounds all exhibited good to moderate antioxidant activity.
{"title":"Syntheses, Characterization and Biological Activity of Coordination Compounds of 3-Hydroxy-2-methyl-4 H -pyran-4-one and Its Mixed Ligand Complexes with 1,2-Diaminocyclohexane","authors":"T. Aiyelabola","doi":"10.4236/ABC.2021.113008","DOIUrl":"https://doi.org/10.4236/ABC.2021.113008","url":null,"abstract":"Coordination compounds of 3-hydroxy-2-methyl-4H-pyran-4-one with iron(III), cobat(III) and chromium(III) were \u0000synthesized with M:L (1:2). Mixed ligand coordination compounds of \u00003-hydroxy-2-methyl-4H-pyran-4-one and 1,2-diaminocyclohexane using the \u0000same metal ions were also synthesized M:L1:L2 (1:1:1) where L1 is 3-hydroxy-2-methyl-4H-pyran-4-one and L2 is \u00001,2-diaminocyclohexane. The coordination compounds obtained were characterized \u0000using electronic and infrared spectral analyses, magnetic susceptibility and \u0000percentage metal analysis. They were also evaluated for their cytotoxic and antioxidant \u0000activities. The result obtained suggested that octahedral geometry was obtained \u0000for all the compounds, as a result of additional two molecules of the solvent \u0000coordinated to the metal ions. Both the primary and secondary ligands \u0000coordinated in a bidentate fashion. The synthesized compounds exhibited \u0000moderate cytotoxicity, although none was as active as the standard. The \u0000cobalt(III) mixed ligand complex elicited the highest activity. The synthesized \u0000compounds all exhibited good to moderate antioxidant activity.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42937202","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
P. Sinda, B. Ponou, B. Tsafack, J. Kühlborn, R. Tchuenguem, R. Teponno, J. Dzoyem, T. Opatz, L. Tapondjou
Phytochemical investigation of the seeds of Pentaclethra macrophylla led to the isolation of a mixture of two new aromatic monoterpene glycosides, pentamacrophylloside A (1a) and pentamacrophylloside B (1b), together with six known secondary metabolites: Comososide (2), secopentaclethroside (3), caffeoylputrescine (4), β-sitosterol-3-O-β-D-glucopyranoside (5), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol (6), and sucrose (7). Their structures were elucidated mainly by extensive spectroscopic analysis (1D and 2D NMR), high-resolution mass spectrometry and by comparison of their spectral data with those of related compounds. The extracts and compounds 3 and 4 were screened for their antimicrobial activity. The n-BuOH fraction showed a weak effect against three microbial strains: Candida albicans (MIC: 256 μg/mL), Enterococcus faecalis (MIC: 512 μg/mL), and Proteus mirabilis (MIC: 512 μg/mL) while no significant inhibition was observed for pure compounds when compared to ketoconazole and ciprofloxacin used as references. Furthermore, the ethnobotany and pharmacology of this plant are reviewed, and the chemophenetic significance of the isolation of the above secondary metabolites is discussed. This is the first report on the isolation of aromatic monoterpenoids from a plant of the genus Pentaclethra.
对大叶五肢草(Pentaclethra macrophylla)种子进行植物化学研究，分离出两种新的芳香单萜苷，五肢草苷a (1a)和五肢草苷B (1b)，以及六种已知的次生代谢物:Comososide(2)、secopentaclethroside(3)、caffeoylpurescine(4)、β-谷甾醇-3- o -β- d -glucopyranoside(5)、2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol(6)和蔗糖(7)。它们的结构主要通过广泛的光谱分析(1D和2D NMR)、高分辨率质谱分析以及与相关化合物的光谱数据比较来确定。对提取物及化合物3、4进行抑菌活性筛选。正丁醇提取物对白色念珠菌(MIC: 256 μg/mL)、粪肠球菌(MIC: 512 μg/mL)和奇异变形杆菌(MIC: 512 μg/mL)的抑菌效果较弱，对纯化合物的抑菌效果与酮康唑和环丙沙星对照无明显差异。综述了该植物的民族植物学和药理学研究进展，并对分离上述次生代谢物的化学意义进行了讨论。本文首次从五草属植物中分离得到芳香单萜类化合物。
{"title":"Ethnobotany, Pharmacology and Phytochemical Investigations of the Seeds of Pentaclethra macrophylla Benth (Mimosaceae)","authors":"P. Sinda, B. Ponou, B. Tsafack, J. Kühlborn, R. Tchuenguem, R. Teponno, J. Dzoyem, T. Opatz, L. Tapondjou","doi":"10.4236/ABC.2021.113009","DOIUrl":"https://doi.org/10.4236/ABC.2021.113009","url":null,"abstract":"Phytochemical investigation of the seeds \u0000of Pentaclethra macrophylla led to the isolation of a mixture of two new aromatic monoterpene \u0000glycosides, pentamacrophylloside A \u0000(1a) and pentamacrophylloside B (1b), \u0000together with six known secondary \u0000metabolites: Comososide (2), secopentaclethroside (3), caffeoylputrescine (4), β-sitosterol-3-O-β-D-glucopyranoside (5), 2-hydroxymethyl-5-(2-hydroxypropan-2-yl)phenol \u0000(6), and sucrose (7). Their structures were elucidated mainly by extensive spectroscopic analysis (1D and \u00002D NMR), high-resolution mass \u0000spectrometry and by comparison of their \u0000spectral data with those of related compounds. The extracts and compounds 3 and 4 were screened for their antimicrobial activity. The n-BuOH \u0000fraction showed a weak effect against three microbial strains: Candida albicans (MIC: 256 μg/mL), Enterococcus faecalis (MIC: 512 μg/mL), and Proteus \u0000mirabilis (MIC: 512 μg/mL) while \u0000no significant inhibition was observed for pure compounds when compared to ketoconazole and ciprofloxacin used as references. Furthermore, the \u0000ethnobotany and pharmacology of this plant are reviewed, and the chemophenetic significance of the isolation of \u0000the above secondary metabolites is discussed. This is the first report on the isolation of aromatic \u0000monoterpenoids from a plant of the genus Pentaclethra.","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43847650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Kandji Pape Matar, Djité Moustapha, Barry Nene Oumou Kesso, Sagne René Ngor, Thioune Marême, Mbacke Ndoumbé Mame, N. H. Malick, G. Fatou, Lopez-Sall Philomène, C. Aynina, D. Amadou, Gueye Papa Madièye
The objective of this study was to assess the association between uric acid and the metabolic syndrome and its components in homozygous sickle cell patients. This is a prospective case/control study of sickle cell SS patients. Each patient was matched to a control of the same sex and age ± 2 years. In our framework, we used the criteria for defining metabolic syndrome according to International Diabetes Federation (IDF) 2009. Assay of all biological parameters was performed with the ARCHITECT ci4100, Abbot (Chicago, Illinois, USA). Data were collected with Excel 2016 software and statistical analysis was done using XLSTAT 2019 software. Student’s T test was used to compare means and a p-value less than 0.05 was considered significant. The study population consisted of 100 homozygous sickle cell patients with an average age of 26 years with a sex ratio of 0.58. The prevalence of metabolic syndrome in our population according to the IDF 2009 was 2%. In our study 28% of patients presented with hyperuricemia. Uricaemia was significantly elevated in patients with components of the metabolic syndrome, in particular in 33% of patients with a large waist circumference, in 25% of hypertensive patients, in 50% of patients with hypertriglyceridemia and in 60% of patients with hypertriglyceridemia and low HDL-cholesterol levels. Significant correlations were found between uricemia and certain components of the metabolic syndrome, in particular the level of triglycerides (r = 0.31, p = 0.002), blood sugar (r = 0.16; p = 0.012), around size (r = 0.071; p ≤ 0.05), HDL-Cholesterol (r = ﹣0.01; p = 0.018), PAS (r = 0.076; p = 0.035) and PAD (r = ﹣0.18; p = 0.0015).
{"title":"Association between Uric Acid and Metabolic Syndrome in Homozygous Sickle Cell Patients","authors":"Kandji Pape Matar, Djité Moustapha, Barry Nene Oumou Kesso, Sagne René Ngor, Thioune Marême, Mbacke Ndoumbé Mame, N. H. Malick, G. Fatou, Lopez-Sall Philomène, C. Aynina, D. Amadou, Gueye Papa Madièye","doi":"10.4236/ABC.2021.113010","DOIUrl":"https://doi.org/10.4236/ABC.2021.113010","url":null,"abstract":"The objective of this study was to assess the association between uric \u0000acid and the metabolic syndrome and its \u0000components in homozygous sickle cell patients. This is a prospective \u0000case/control study of sickle cell SS patients. Each patient \u0000was matched to a control of the same sex and age ± 2 years. In our framework, we used the criteria for defining metabolic syndrome according to \u0000International Diabetes Federation (IDF) 2009. Assay of all biological parameters was performed with the ARCHITECT ci4100, Abbot (Chicago, \u0000Illinois, USA). Data were collected with Excel 2016 software and statistical \u0000analysis was done using XLSTAT 2019 software. Student’s T test was \u0000used to compare means and a p-value less \u0000than 0.05 was considered significant. The study \u0000population consisted of 100 homozygous sickle cell patients with an average age \u0000of 26 years with a sex ratio of 0.58. The prevalence of metabolic syndrome in \u0000our population according to the IDF 2009 was 2%. In our study 28% of patients \u0000presented with hyperuricemia. Uricaemia was significantly elevated in patients \u0000with components of the metabolic syndrome, in particular in 33% of patients \u0000with a large waist circumference, in 25% of hypertensive patients, in 50% of \u0000patients with hypertriglyceridemia and in 60% of patients with hypertriglyceridemia \u0000and low HDL-cholesterol levels. Significant correlations were found between \u0000uricemia and certain components of the metabolic syndrome, in particular the \u0000level of triglycerides (r = 0.31, p = 0.002), blood sugar (r = 0.16; p = 0.012), \u0000around size (r = 0.071; p ≤ 0.05), HDL-Cholesterol (r = ﹣0.01; p = 0.018), PAS (r = 0.076; p = 0.035) and PAD (r = ﹣0.18; p = \u00000.0015).","PeriodicalId":59114,"journal":{"name":"生物化学进展(英文)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48271543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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