Pub Date : 2024-08-23DOI: 10.1007/s13197-024-06064-3
Antonietta Baiano, Anna Fiore, Mariacinzia Rutigliano, Barbara la Gatta
This work was aimed to investigate the effects of three factors on cookie quality: brewers’ spent grain (BSG) composition [65% malted barley and 35% of unmalted durum (DA) or soft (RI), or emmer (EM) wheats]; geographical origin of the cereals used in brewing (Daunia or Salento); and percentages of BSG in cookie formulation (30 or 40%). A control made of 100% Manitoba flour was produced. Statistical analyses were performed to evaluate the effects of those factors (Analysis of Variance), the possibility to distinguish the various types of cookies (Principal Component Analysis), and the relationships among variables (Pearson Correlation Analysis).The single and interactive effects of the three factors were significant for almost all variables. Cookies with 40% EM spent grains showed the highest ash, dietary fibre, and total phenolic contents but cookies with 30% DA or RI spent grains received the highest overall quality scores due to the higher intensity of their fresh baked flavour and their lower hardness and fibrousness. Based on the nutritional and sensory characteristics, cookies fortified with RI and DA were the best to consume. Although few physicochemical differences can be attributed to geographical origin, a slightly higher overall sensory score was assigned to those produced with Salento cereals. Principal Component Analysis showed a clear separation between the control made of 100% Manitoba flour and the group of fortified cookies. Among the latter, the cookies produced with RI and DA spent grains were indistinguishable from each other due to their similar quality characteristics.
这项工作旨在研究以下三个因素对饼干质量的影响:酿酒师用过的谷物(BSG)成分[65%的发芽大麦和 35%的未发芽硬质小麦(DA)或软质小麦(RI)或埃默尔小麦(EM)];酿酒所用谷物的地理来源(道尼亚或萨兰托);以及饼干配方中 BSG 的百分比(30% 或 40%)。此外,还制作了由 100% 马尼托巴面粉制成的对照组。进行了统计分析,以评估这些因素的影响(方差分析)、区分不同类型饼干的可能性(主成分分析)以及变量之间的关系(皮尔逊相关分析)。含 40% EM 废谷物的饼干灰分、膳食纤维和总酚类含量最高,但含 30% DA 或 RI 废谷物的饼干总体质量得分最高,原因是其新鲜烘焙风味更浓,硬度和纤维度较低。从营养和感官特征来看,添加了 RI 和 DA 的饼干最适于食用。虽然几乎没有理化差异可归因于地理来源,但使用萨兰托谷物生产的饼干的总体感官评分略高。主成分分析表明,100% 马尼托巴面粉制成的对照组与强化饼干组之间存在明显差异。在后者中,用 RI 和 DA 废谷物生产的饼干因其相似的质量特性而无法区分。
{"title":"Application of a multivariate approach to the study of chemometric and sensory profiles of cookies fortified with brewers’ spent grain","authors":"Antonietta Baiano, Anna Fiore, Mariacinzia Rutigliano, Barbara la Gatta","doi":"10.1007/s13197-024-06064-3","DOIUrl":"https://doi.org/10.1007/s13197-024-06064-3","url":null,"abstract":"<p>This work was aimed to investigate the effects of three factors on cookie quality: brewers’ spent grain (BSG) composition [65% malted barley and 35% of unmalted durum (DA) or soft (RI), or emmer (EM) wheats]; geographical origin of the cereals used in brewing (Daunia or Salento); and percentages of BSG in cookie formulation (30 or 40%). A control made of 100% Manitoba flour was produced. Statistical analyses were performed to evaluate the effects of those factors (Analysis of Variance), the possibility to distinguish the various types of cookies (Principal Component Analysis), and the relationships among variables (Pearson Correlation Analysis).The single and interactive effects of the three factors were significant for almost all variables. Cookies with 40% EM spent grains showed the highest ash, dietary fibre, and total phenolic contents but cookies with 30% DA or RI spent grains received the highest overall quality scores due to the higher intensity of their fresh baked flavour and their lower hardness and fibrousness. Based on the nutritional and sensory characteristics, cookies fortified with RI and DA were the best to consume. Although few physicochemical differences can be attributed to geographical origin, a slightly higher overall sensory score was assigned to those produced with Salento cereals. Principal Component Analysis showed a clear separation between the control made of 100% Manitoba flour and the group of fortified cookies. Among the latter, the cookies produced with RI and DA spent grains were indistinguishable from each other due to their similar quality characteristics.</p>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194008","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-22DOI: 10.1007/s13197-024-06062-5
Xia Ma, Zhihan Liu, Yan He, Yumei Zhou
Natto is a functional food, but it produces an unpleasant smell during the fermentation process. To eliminate the unacceptable smell, Lactobacillus bulgaricus and Bacillus subtilis were used to co-ferment soybeans in this study. The optimal conditions of co-fermentation anaerobic fermentation were: fermentation time 20 h, temperature 35 °C, strain ratio 1:1, and inoculum amount 4%. After optimization, fibrinolytic activity, antioxidant activity, polyphenol content, and γ-polyglutamic acid (γ-PGA) yield of Natto were increased by 15.2%, 10.9%, 29.1%, and 72.3%. It also introduced a wealth of probiotics with lactic acid bacteria up to 1.45 X 1010cfu/g. The co-fermented natto had unique fruity and lactic flavor, and high sensory score based on the flavor and sensory analysis. The activity and value of natto have been increased through co-fermentation, making it possible to improve natto.
{"title":"Process optimization of co-fermentation natto with Lactobacillus bulgaricus and characteristic analysis","authors":"Xia Ma, Zhihan Liu, Yan He, Yumei Zhou","doi":"10.1007/s13197-024-06062-5","DOIUrl":"https://doi.org/10.1007/s13197-024-06062-5","url":null,"abstract":"<p>Natto is a functional food, but it produces an unpleasant smell during the fermentation process. To eliminate the unacceptable smell, <i>Lactobacillus bulgaricus</i> and <i>Bacillus subtilis</i> were used to co-ferment soybeans in this study. The optimal conditions of co-fermentation anaerobic fermentation were: fermentation time 20 h, temperature 35 °C, strain ratio 1:1, and inoculum amount 4%. After optimization, fibrinolytic activity, antioxidant activity, polyphenol content, and γ-polyglutamic acid (γ-PGA) yield of Natto were increased by 15.2%, 10.9%, 29.1%, and 72.3%. It also introduced a wealth of probiotics with lactic acid bacteria up to 1.45 X 10<sup>10</sup>cfu/g. The co-fermented natto had unique fruity and lactic flavor, and high sensory score based on the flavor and sensory analysis. The activity and value of natto have been increased through co-fermentation, making it possible to improve natto.</p>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Fermentation of milk contributes to an array of organoleptic and nutritional changes, which is largely governed by the metabolic activity of starter cultures. Dahi, a popular traditional fermented milk product of India, varies considerably from its commercial counterparts due to diversity in household production practices and the use of undefined microbial consortia as inoculants. Herein, we investigated the unexplored bacterial signatures and metabolic profiles of traditional Dahi from northern region of India using 16S metagenomics and untargeted gas chromatography-mass spectrophotometry. We found Firmicutes followed by Proteobacteria as the dominant phyla, while Lactobacillus and Streptococcus were the most prevalent lactic acid bacterial genera many species of which also serves as thermophilic starters. Notably, differential abundance of environmental bacteria like Bacilli, Prevotella, Oscillospira, Ruminococcus, and Acinetobacter were also observed in different samples. The study also revealed a total of 62 metabolites belonging to amino acids, fatty acids, sugars, and others metabolic intermediates, which also varied among regions. This highlights different production practices as influenced by milk type, heating methods, container choices and inoculum types leading to distinct microbiota succession and associated metabolites. This study provides valuable insights into the complexities as well as health benefits of indigenous fermented milks particularly Dahi and paves the way for future exploration of novel probiotic strains.
Graphical abstract
牛奶发酵会导致一系列感官和营养变化,这在很大程度上取决于发酵培养物的新陈代谢活动。Dahi 是印度一种流行的传统发酵奶产品,由于家庭生产方式的多样性以及使用未确定的微生物群作为接种物,它与商业同类产品有很大的不同。在此,我们使用 16S 元基因组学和非靶向气相色谱-质谱光度法研究了印度北部地区传统 Dahi 中尚未探索的细菌特征和代谢特征。我们发现,固着菌属和变形菌属是最主要的菌属,而乳酸菌和链球菌是最常见的乳酸菌属,其中许多种类还可用作嗜热起始菌。值得注意的是,在不同的样本中还观察到了不同数量的环境细菌,如芽孢杆菌、普雷沃特氏菌、弧菌、反刍球菌和醋杆菌。研究还发现了氨基酸、脂肪酸、糖类和其他代谢中间产物共 62 种代谢物,这些代谢物在不同地区也存在差异。这凸显了不同的生产方式受牛奶类型、加热方法、容器选择和接种物类型的影响,导致了不同的微生物群演替和相关代谢物。这项研究为了解本土发酵奶(尤其是 Dahi)的复杂性和健康益处提供了宝贵的见解,并为今后探索新型益生菌菌株铺平了道路。
{"title":"16S metagenomics and metabolomics unveil the microbial compositions and metabolite profiles in Dahi, a traditional Indian fermented milk product prepared by the backslopping method","authors":"Amrita Tigga, Rashmi Hogarehalli Mallappa, Santhosh Kumar Muniyappa, Saurabh Kadyan, Diwas Pradhan, E. Shree Niharika, Sunita Grover","doi":"10.1007/s13197-024-06050-9","DOIUrl":"https://doi.org/10.1007/s13197-024-06050-9","url":null,"abstract":"<p>Fermentation of milk contributes to an array of organoleptic and nutritional changes, which is largely governed by the metabolic activity of starter cultures. <i>Dahi</i>, a popular traditional fermented milk product of India, varies considerably from its commercial counterparts due to diversity in household production practices and the use of undefined microbial consortia as inoculants. Herein, we investigated the unexplored bacterial signatures and metabolic profiles of traditional <i>Dahi</i> from northern region of India using 16S metagenomics and untargeted gas chromatography-mass spectrophotometry. We found <i>Firmicutes</i> followed by <i>Proteobacteria</i> as the dominant phyla, while <i>Lactobacillus</i> and <i>Streptococcu</i>s were the most prevalent lactic acid bacterial genera many species of which also serves as thermophilic starters. Notably, differential abundance of environmental bacteria like <i>Bacilli, Prevotella, Oscillospira, Ruminococcus</i>, and <i>Acinetobacter</i> were also observed in different samples. The study also revealed a total of 62 metabolites belonging to amino acids, fatty acids, sugars, and others metabolic intermediates, which also varied among regions. This highlights different production practices as influenced by milk type, heating methods, container choices and inoculum types leading to distinct microbiota succession and associated metabolites. This study provides valuable insights into the complexities as well as health benefits of indigenous fermented milks particularly <i>Dahi</i> and paves the way for future exploration of novel probiotic strains.</p><h3 data-test=\"abstract-sub-heading\">Graphical abstract</h3>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194034","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
In this study, the extract and powder obtained from the Reishi mushroom (Ganoderma lucidum) were used in the production of yogurt, and also Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis bacteria were also inoculated. Furthermore, all values were also affected in parallel with the increase in the added ratio. Reishi mushroom powder and extract also increased the samples’ total antioxidant and phenolic values. Among the samples, the highest total phenolic content was found in the 2EA-coded sample with 2.415 mg GAE/g and the lowest in the control sample with 0.630 mg GAE/g. The highest antioxidant activity was determined in the 2PA-coded sample with a value of 0.198. The powder and extract showed a prebiotic effect by contributing positively to the development of probiotic bacteria added to yogurt. The count of probiotic bacteria in yogurts with Reishi mushroom powder and extract increased by approximately 1.4 log cfu/g. Using powder and extract of the Reishi mushroom in yogurt production gave yogurt significant functionality.
{"title":"Incorporating Ganoderma lucidum extract and powder with probiotic cultures (Lactobacillus acidophilus and Bifidobacterium animalis subsp. lactis) enhanced the functional, textural, and sensory qualities of yogurt","authors":"Azize Atik, İlker Atik, Gökhan Akarca, Ayşe Janseli Denizkara","doi":"10.1007/s13197-024-06068-z","DOIUrl":"https://doi.org/10.1007/s13197-024-06068-z","url":null,"abstract":"<p>In this study, the extract and powder obtained from the Reishi mushroom (<i>Ganoderma lucidum</i>) were used in the production of yogurt, and also <i>Lactobacillus acidophilus</i> and <i>Bifidobacterium animalis</i> subsp. <i>lactis</i> bacteria were also inoculated. Furthermore, all values were also affected in parallel with the increase in the added ratio. Reishi mushroom powder and extract also increased the samples’ total antioxidant and phenolic values. Among the samples, the highest total phenolic content was found in the 2EA-coded sample with 2.415 mg GAE/g and the lowest in the control sample with 0.630 mg GAE/g. The highest antioxidant activity was determined in the 2PA-coded sample with a value of 0.198. The powder and extract showed a prebiotic effect by contributing positively to the development of probiotic bacteria added to yogurt. The count of probiotic bacteria in yogurts with Reishi mushroom powder and extract increased by approximately 1.4 log cfu/g. Using powder and extract of the Reishi mushroom in yogurt production gave yogurt significant functionality.</p>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194012","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-18DOI: 10.1007/s13197-024-06035-8
Ferhat Yuksel, Sümeyye Çağlar
In the present study, einkorn wheat flour (Tiriticum monococcum L.) was incorporated into a special dried bread (peksimet) formulation produced from sourdough breads at different concentrations (0–10–20–30–40 and 50 g 100 g−1) and some physicochemical and nutritional (total dietary fiber, resistant starch, glycemic index, acrylamide content) characteristics and sensory properties of the samples were investigated. The total dietary fiber content of the bread samples ranged from 3.00 to 6.17 g 100 g−1. The highest acrylamide content (247.54 µg/kg) was obtained using an einkorn flour level of 40 g 100 g−1. Einkorn wheat flour resulted in a significant decrease (from 94.61 to 89.23) in the glycemic index level of the bread samples (p < 0.05). Bread enriched with einkorn wheat flour (50.0 g 100 g−1) received the highest overall acceptability score. In conclusion, einkorn wheat flour could be used in a special dried bread formulation to enhance its nutritional quality.
{"title":"In vitro glycemic index, acrylamide content, and some physicochemical and sensorial properties of special dried bread (Peksimet) enriched with einkorn wheat (Tiriticum monococcum L.) flour","authors":"Ferhat Yuksel, Sümeyye Çağlar","doi":"10.1007/s13197-024-06035-8","DOIUrl":"https://doi.org/10.1007/s13197-024-06035-8","url":null,"abstract":"<p>In the present study, einkorn wheat flour (<i>Tiriticum monococcum</i> L.) was incorporated into a special dried bread (peksimet) formulation produced from sourdough breads at different concentrations (0–10–20–30–40 and 50 g 100 g<sup>−1</sup>) and some physicochemical and nutritional (total dietary fiber, resistant starch, glycemic index, acrylamide content) characteristics and sensory properties of the samples were investigated. The total dietary fiber content of the bread samples ranged from 3.00 to 6.17 g 100 g<sup>−1</sup>. The highest acrylamide content (247.54 µg/kg) was obtained using an einkorn flour level of 40 g 100 g<sup>−1</sup>. Einkorn wheat flour resulted in a significant decrease (from 94.61 to 89.23) in the glycemic index level of the bread samples (<i>p</i> < 0.05). Bread enriched with einkorn wheat flour (50.0 g 100 g<sup>−1</sup>) received the highest overall acceptability score. In conclusion, einkorn wheat flour could be used in a special dried bread formulation to enhance its nutritional quality.</p>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194052","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-17DOI: 10.1007/s13197-024-06058-1
Lakshmi Jyothish, Sameera Kazi, Jyoti S. Gokhale
Safe and healthy food is the fundamental right of every citizen. Problems caused by foodborne pathogens have always raised a threat to food safety and human health. Centers for Disease Control and Prevention (CDC) estimates that around 48 million people are affected by food intoxication, and 3000 people succumb to death. Hence, it is inevitable that an approach that is efficient, reliable, sensitive, and rapid approach that can replace the conventional analytical methods such as microbiological and biochemical methods, high throughput next-generation sequence (NGS), polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA), etc. Even though the accuracy of conventional methods is high, it is tedious; increased consumption of reagents/samples, false positives, and complex operations are the drawbacks of these methods. Microfluidic devices have shown remarkable advances in all branches of science. They serve as an alternative to conventional ways to overcome the abovementioned drawbacks.
Furthermore, coupling microfluidics can improve the efficiency and accuracy of conventional methods such as surface plasma resonance, loop-mediated isothermal amplification, ELISA, and PCR. This article reviewed the progress of microfluidic devices in the last ten years in detecting foodborne pathogens. Microfluidic technology has opened the research gateway for developing low-cost, on-site, portable, and rapid assay devices. The article includes the application of microfluidic-based devices to identify critical food pathogens and briefly discusses the necessary research in this area.
{"title":"Microfluidics for detection of food pathogens: recent trends and opportunities","authors":"Lakshmi Jyothish, Sameera Kazi, Jyoti S. Gokhale","doi":"10.1007/s13197-024-06058-1","DOIUrl":"https://doi.org/10.1007/s13197-024-06058-1","url":null,"abstract":"<p>Safe and healthy food is the fundamental right of every citizen. Problems caused by foodborne pathogens have always raised a threat to food safety and human health. Centers for Disease Control and Prevention (CDC) estimates that around 48 million people are affected by food intoxication, and 3000 people succumb to death. Hence, it is inevitable that an approach that is efficient, reliable, sensitive, and rapid approach that can replace the conventional analytical methods such as microbiological and biochemical methods, high throughput next-generation sequence (NGS), polymerase chain reaction (PCR), and enzyme-linked immunosorbent assay (ELISA), etc. Even though the accuracy of conventional methods is high, it is tedious; increased consumption of reagents/samples, false positives, and complex operations are the drawbacks of these methods. Microfluidic devices have shown remarkable advances in all branches of science. They serve as an alternative to conventional ways to overcome the abovementioned drawbacks.</p><p>Furthermore, coupling microfluidics can improve the efficiency and accuracy of conventional methods such as surface plasma resonance, loop-mediated isothermal amplification, ELISA, and PCR. This article reviewed the progress of microfluidic devices in the last ten years in detecting foodborne pathogens. Microfluidic technology has opened the research gateway for developing low-cost, on-site, portable, and rapid assay devices. The article includes the application of microfluidic-based devices to identify critical food pathogens and briefly discusses the necessary research in this area.</p>","PeriodicalId":632,"journal":{"name":"Journal of Food Science and Technology","volume":null,"pages":null},"PeriodicalIF":2.701,"publicationDate":"2024-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142194013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}