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2009 3rd International Conference on Bioinformatics and Biomedical Engineering最新文献

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Antitumor Activity of Escin in Mice Cervical Carcinoma U_(14) In Vivo and Human Cervical Carcinoma Hela Cell Line In Vitro Escin对小鼠宫颈癌U_(14)和人宫颈癌Hela细胞株体内抗肿瘤活性的研究
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162378
Xueying Zhou, Changhai Wang, F. Fu
Pollution made cancer a major disease threatening people's health. In the search for new cancer drug treatments, natural products perform an essential role. Escin, a mixture of triterpene saponins extracted from Aesculus Wilsonii Rehd., was used to analyze the cell growth inhibitory effects and the induction of apoptosis in human cervical carcinoma Hela cancer cell line in vitro. The results of MTT cell viability assay showed that escin could induce significant concentration-dependent and time-dependent inhibition of cell growth, and the cell cycle was arrested at G 1 /S phase in the escin-treated cells. Apoptosis were detected and assessed by Acridine Orange/Ethidium Bromide Staining and Flow Cytometry. The antitumor efficacy of escin on mice cervical carcinoma U_(14) in vivo was investigated, at dose of 2.8 mg/kg escin had rather high inhibition ratio(43.5%) on U_(14) tumor growth.
污染使癌症成为威胁人们健康的主要疾病。在寻找新的癌症药物治疗中,天然产物发挥着至关重要的作用。Escin是一种由三萜皂苷组成的混合物。体外实验分析其对人宫颈癌Hela细胞株的细胞生长抑制作用及诱导凋亡的作用。MTT细胞活力测定结果显示,叶香素对细胞生长有明显的浓度依赖性和时间依赖性抑制作用,细胞周期停留在g1 /S期。用吖啶橙/溴化乙啶染色和流式细胞术检测细胞凋亡。研究了escin对小鼠宫颈癌U_(14)的体内抗肿瘤作用,在2.8 mg/kg剂量下,escin对U_(14)肿瘤生长有较高的抑制率(43.5%)。
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引用次数: 0
Urban Water Sustainable Utilization 城市水资源可持续利用
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5163732
H. Cui, Jun Liu, C. Gao, Fangqing Yu
The prevalent water problems like quantity shortage, environment pollution and ecological damage restrict urban sustainable development. Unreasonable water utilizing causes conflicts between artificial water cycling system and natural cycling water system, which turns out to be the source of urban water problems. To find ultimate solutions to the water problems and the way of sustainable water utilization, artificial water cycling system with the whole urban water ecosystem is analyzed. After a comparative analysis of urban water ecosystem and steady natural ecosystem, unsmooth flowing and unbalanced in-out of matter is put forward as the inducement of water problems. It is quite necessary to strengthen wastewater-disposal making it accord with exploitation and using, and to strengthen water renewal forming efficient circulation. In all, water utilizing model should be completed. Reduce, reuse, recycle principle can be applied to promote water cycling efficiency. For further study wastewater can be utilized as resources with technical innovation.
水资源短缺、环境污染、生态破坏等问题普遍存在,制约着城市的可持续发展。不合理的水资源利用导致人工循环水系统与自然循环水系统之间的冲突,成为城市水问题的根源。为从根本上解决城市水问题,寻找可持续利用水资源的途径,对城市整体水生态系统的人工水循环系统进行了分析。通过对城市水生态系统与稳定的自然生态系统的对比分析,提出了不顺畅的流动和不平衡的物质进出是水问题的诱因。加强废水处理,使其与开发利用相适应,加强水的更新,形成高效循环是十分必要的。总之,应完善水资源利用模式。减量、再利用、再循环原则可用于提高水循环效率。通过技术创新,废水可以作为资源加以利用。
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引用次数: 1
Microcosm Study on Response of Soil Microorganisms to Polycyclic Aromatic Hydrocarbons Contamination 土壤微生物对多环芳烃污染响应的微观研究
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5163052
Dan Zhang, A. Ding, Wenjie Tan, Cheng Chen, Lirong Cheng, Yujiao Sun, Xue-yu Lin
Soil and groundwater contamination by petroleum and coal tar becomes one of the urgent environmental issues concerned by more and more scientists with regards to the public health. With the induction of organic contaminants to soil and groundwater it will change the environmental conditions within the plume and hence lead to the variation of indigenous microorganisms. The article aims to investigate the response of indigenous microorganisms to the petroleum contamination. Three polycyclic aromatic hydrocarbons (PAHs) phenanthrene, anthracene and pyrene were selected as the contaminants and tested using laboratory microcosm. The response of indigenous microorganisms to the PAHs induction was analyzed by measuring the change of soil microbial activity, soil catalase activity, contaminants concentration and geochemical parameters. The experimental results showed that: (1) the major parts of the PAHs were absorbed by the soil, its transport was retarded, (2) the soil microbial activity was inhibited at the initial stage of PAHs induction and then slowly increased, (3) the soil catalase activity was stimulated at first with PAHs induction and then inhibited within the range -0.05 mL (0.1 M KMnO 4 )/g (soil).
石油和煤焦油对土壤和地下水的污染已成为越来越多科学家关注的环境问题之一,关系到公众健康。随着有机污染物进入土壤和地下水,它将改变烟羽内的环境条件,从而导致本地微生物的变化。研究了石油污染对土壤微生物的影响。选取3种多环芳烃(PAHs):菲、蒽和芘作为污染物,采用实验室微观环境进行检测。通过测定土壤微生物活性、土壤过氧化氢酶活性、污染物浓度和地球化学参数的变化,分析了本地微生物对多环芳烃诱导的响应。实验结果表明:(1)多环芳烃的主要部分被土壤吸收,其转运受到阻碍;(2)多环芳烃诱导初期土壤微生物活性受到抑制后缓慢升高;(3)多环芳烃诱导先刺激土壤过氧化氢酶活性,然后在-0.05 mL (0.1 M kmno4)/g(土壤)范围内抑制土壤过氧化氢酶活性。
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引用次数: 1
Investigation of Resistivity's Abnormity and Moduli of Elasticity Softening in NiTi Shape Memory Alloy NiTi形状记忆合金的电阻率异常及弹性软化模量研究
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162982
F. M. Chen, G. X. Zhao, L. S. Zhang, J. Y. Guo, H. X. Yang, Z. M. Li
Spreading the landau phase change theory, by using two order parameters, that is martensite transition percentange Φ and mean shearing strain ε, and supposing friction function is the first-order linear coupling of them. Explaining the experimental facts of resistivity’s abnormal raising and moduli of elasticity’s softening on the Thermoelastic Martensitic Tansformation. Keywords-phase transformations; stress; thermoelastic martensite; phenomenological theory ; Shape memory effect
采用马氏体转变百分比Φ和平均剪切应变ε两个阶参数,假设摩擦函数是它们的一阶线性耦合,推广朗道相变理论。解释了热弹性马氏体相变中电阻率异常升高和弹性模量软化的实验事实。Keywords-phase转换;压力;热弹性马氏体;现象学理论;形状记忆效应
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引用次数: 1
Effect of Contralateral Stimulation on Auditory Filter Bandwidth: A Preliminary Study 对侧刺激对听觉过滤带宽影响的初步研究
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162691
Chang Liu
The main goal of this study was to examine the effect of contralateral stimulation on auditory filter shape. Auditory filter was measured at 1 and 2 kHz for normal-hearing listeners using notched-noise method with and without contralateral stimulation presented. A broadband noise was presented as contralateral stimulation at three levels: 50, 60, and 70 dB SPL. Results indicated that contralateral stimulation at 50 dB SPL slightly increased frequency selectivity (i.e. reduced auditory filter bandwidth) for 2 kHz, suggesting that auditory efferent fibers such as medial olivocochlear bundles may function to improve frequency selectivity of the cochlear. Such improvement in the cochlear frequency selectivity appeared to be dependent on signal frequency and intensity of contralateral stimulation.
本研究的主要目的是探讨对侧刺激对听觉滤过器形状的影响。在有和无对侧刺激的情况下,用陷波噪声法测量正常听力听者在1和2 kHz的听觉滤波器。宽带噪声作为对侧刺激在三个水平:50、60和70 dB SPL。结果表明,对侧50 dB SPL的刺激在2 kHz时略微增加了频率选择性(即减少了听觉滤波器带宽),提示耳蜗内侧耳蜗束等听觉传出纤维可能起着提高耳蜗频率选择性的作用。这种耳蜗频率选择性的改善似乎依赖于对侧刺激的信号频率和强度。
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引用次数: 0
Development and Validity of Tissue Biomechanics Modeling for Virtual Robot Assisted Orthopedic Surgery System 虚拟机器人辅助骨科手术系统组织生物力学建模的开发与有效性
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162638
Monan Wang
Information with respect to the internal stresses/strains in the human leg during bone-setting is helpful to control the medical robots in HIT-robot assisted orthopedic surgery system (HIT-RAOS). While techniques to measure external pressure are well established, direct measurement of the internal stresses/strains is difficult. A three-dimensional (3D) finite element model of the human leg is developed using the actual geometry of the leg skeleton and soft tissues, which were obtained from 3D reconstruction of CT images. The major tendons are simulated using tension-only truss elements by connecting the corresponding attachment points on the bone surfaces. The bony structures are defined as linearly elastic, while the soft tissue and muscles are assumed to be hyper elastic. The bony and tendons structures are embedded in a volume of soft tissues. To validate this model, the same cadaver is utilized in experiment, during reposition procedure, the force needed to separate the two broken bones and corresponding the distance are recorded. Then, another set of data is acquired from the finite element analysis. After comparison, the finite element model is proved to be acceptable. This simulation model is used in virtual HIT-robot assisted orthopedic surgery system.
在HIT-robot辅助骨科手术系统(HIT-RAOS)中,关于人腿在植骨过程中的内应力/应变信息有助于医疗机器人的控制。虽然测量外部压力的技术已经建立,但直接测量内部应力/应变是困难的。通过对CT图像进行三维重建,得到人体腿部骨骼和软组织的实际几何形状,建立了人体腿部的三维有限元模型。通过连接骨表面相应的附着点来模拟主要肌腱。骨结构被定义为线性弹性,而软组织和肌肉被认为是超弹性。骨和肌腱结构嵌入大量的软组织中。为了验证该模型,实验中使用同一具尸体,在复位过程中,记录两根骨折分离所需的力和相应的距离。然后,从有限元分析中获得另一组数据。通过比较,证明了有限元模型是可以接受的。将该仿真模型应用于虚拟hit机器人辅助骨科手术系统中。
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引用次数: 3
Cultivation of High Cell Density to Improve Degradation Efficiency of River-Water 培养高密度细胞提高河水降解效率
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162566
Su-yun Chang, Jingmei Sun, Jianjun Huang, Baosheng Sun, P. Sun
Technology of bioaugmentation was used to degrade contaminants of river water. It was realized that degradation effectives was affected by the density of added microbes. Improving the density of microbes was necessary to reach satisfactory degradation effects on the contaminated river. Therefore a practical recycle cultivation method was studied. It was proved to be practical and effective, for easy to combine with air-lift bioreactor, and the process was easy to implement and take low cost. To achieve high cell density and keep them with high activity, three times cycle was the optimum. It was noticed that recycle cultivation was affected by centrifuge temperature and carbon concentration. More carbon source and higher centrifugal temperature were required to harvest high cell density. But for degradation ability of individual microbe, more carbon was not effective. Finally recycle culture was ended at low centrifugal temperature or higher, because a lot of gels were produced. The gels wrap to the cells so firmly that the cells can't uptake the nutrient and their activities were limited.
采用生物强化技术对河流水体中的污染物进行了降解。研究发现,添加微生物的密度对降解效果有影响。提高微生物浓度对污染河流的降解效果是必要的。为此,研究了一种实用的循环栽培方法。实践证明,该工艺易于与气升式生物反应器相结合,操作简单,成本低,具有实用性和有效性。为获得较高的细胞密度并保持较高的活性,3次循环为最佳。注意到循环培养受离心温度和碳浓度的影响。为了获得高细胞密度,需要更多的碳源和更高的离心温度。但对于单个微生物的降解能力而言,碳含量的增加并不有效。最后,由于产生了大量的凝胶,在较低或更高的离心温度下结束循环培养。凝胶紧紧地包裹在细胞上,细胞无法吸收营养物质,它们的活动受到限制。
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引用次数: 0
Bioavailable Vanadium Extracting with EDTA in Panzhihua Urban Park Topsoil EDTA萃取攀枝花城市公园表土中生物可利用性钒
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162440
Yanguo Teng, J.Y. Xiao, Q. Zhang, R. Zuo, Jinsheng Wang, Jie Yang
Vanadium was a trace element, which may be biologically beneficial and possibly essential but certainly harmful to human beings and some living organisms if excessive. In order to investigate potentially environmental risk of vanadium, a total of 46 topsoil samples were collected in dry and wet season in Panzhihua urban park. The concentration of bioavailable vanadium in wet season was higher than that in the dry season. The higher concentration of bioavailable vanadium was distributed in the gateway, the children's pleasure ground and the resting place. In contrast to the toxicity reference values (TRV) in Eco-SSLs for vanadium in the soil and bioavailable vanadium, there was a slight health risk. So some countermeasures of reducing pollution of vanadium in urban park soil should be put forward.
钒是一种微量元素,在生物学上可能是有益的,可能是必需的,但如果过量,对人体和某些生物肯定是有害的。为探讨钒的潜在环境风险,在攀枝花城市公园干湿季节共采集了46份表土样品。湿季生物可利用钒浓度高于旱季。生物可利用钒浓度较高的区域分布在入口、儿童游乐场和休息场所。与生态ssls中土壤中钒和生物可利用钒的毒性参考值(TRV)相比,存在轻微的健康风险。因此,应提出降低城市公园土壤中钒污染的对策。
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引用次数: 0
Effects of Sewage Sludge Application on Growth of Maize Grown in Aluminum-Toxic Soils 污泥施用对铝中毒土壤中玉米生长的影响
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5162753
Qiang Liu, X. Zou, C. Xiao, L. Luo
In the present study, a pot experiment was conducted to investigate the ameliorative effects of sewage sludge on maize grown in acid soils by measuring plant growth and physiological responses. The sewage sludge had higher pH and organic matter than red soil, thus the application of sewage sludge significantly increased soil pH and decreased monomeric aluminum (Al) concentration in soil solution. In general, there was a trend of increasing plant height, fresh weight, chlorophyll (Chi) content and activities of peroxides (POD) and catalase (CAT), or decreasing contents of malondialdehyde (MDA) and membrane permeability (MP), with sewage sludge ratio increased from 0% to 10%. However, those index mentioned above were opposite at a higher sewage sludge application ratio, ie. 30%, partly due to the high contents of soluble salts and NH 4 -N in sewage sludge. The physiological amelioration (such as keeping higher activities of POD and CAT, more chlorophyll content and lower contents of MDA and MP) for maize grown in Al-toxic soils at 10% sewage sludge ratio provides foundations for the tolerance to Al, thus resulting in highest plant growth.
本研究通过盆栽试验,考察了污水污泥对酸性土壤中玉米生长和生理反应的改善作用。污泥的pH值和有机质均高于红壤,因此污泥的施用显著提高了土壤pH值,降低了土壤溶液中单体铝(Al)的浓度。总体而言,随着污泥比从0%增加到10%,株高、鲜重、叶绿素(Chi)含量、过氧化物(POD)和过氧化氢酶(CAT)活性增加,丙二醛(MDA)含量和膜透性(MP)含量降低。而污泥施用量越大,上述指标则相反。30%,部分原因是污水污泥中可溶性盐和nh4 -N含量高。10%污泥比铝毒土壤中玉米的生理改良(保持较高的POD和CAT活性,较高的叶绿素含量,较低的MDA和MP含量)为玉米耐铝提供了基础,从而使植株生长达到最高水平。
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引用次数: 4
Utilization of Spatial Coherence in Functional Neuroimage-Based Classification 空间相干性在功能性神经图像分类中的应用
Pub Date : 2009-06-11 DOI: 10.1109/ICBBE.2009.5163742
Pinaki S. Mitra, Vanathi Gopalakrishnan, R. McNamee
Functional magnetic resonance imaging provides a non-invasive mechanism for monitoring brain activity of subjects during performance of a task. While this approach has been used extensively for human brain mapping activities, automated classification of subjects based on neural activation patterns is also of interest. However, due to the high dimensionality of the image data, classification accuracy is highly dependent upon the adequacy of the features used in the models. In this work 1 , we present a new feature refinement strategy that uses spatial coherence information to eliminate irrelevant features from consideration. For a neurobehavioral disinhibition dataset, we show that this new approach for feature selection using spatially coherent voxels (SCV) outperforms conventional methods.
功能磁共振成像提供了一种非侵入性机制来监测受试者在执行任务时的大脑活动。虽然这种方法已广泛用于人脑映射活动,但基于神经激活模式的主题自动分类也很有趣。然而,由于图像数据的高维,分类精度高度依赖于模型中使用的特征的充分性。在这项工作中,我们提出了一种新的特征细化策略,该策略使用空间相干信息来消除不相关的特征。对于神经行为去抑制数据集,我们表明这种使用空间相干体素(SCV)进行特征选择的新方法优于传统方法。
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引用次数: 0
期刊
2009 3rd International Conference on Bioinformatics and Biomedical Engineering
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