Z. A. Rahman, Sentoor Kumaran Govindasamy, Ahmad Ngalim, Nur Auni Syazalien Adlan, N. Basiron, A. N. Othman
{"title":"Callus Induction of Young Leaf Coconut cv. MATAG with Combination of 2,4-Dichlorophenoxyacetic Acid (2,4-D), α-Naphthalene Acetic Acid (NAA) and Benzyl Amino Purin (BAP)","authors":"Z. A. Rahman, Sentoor Kumaran Govindasamy, Ahmad Ngalim, Nur Auni Syazalien Adlan, N. Basiron, A. N. Othman","doi":"10.4236/abb.2022.135015","DOIUrl":"https://doi.org/10.4236/abb.2022.135015","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Somo Toukam Gabriel Mahbou, Godswill Ntsomboh-Ntsefong, Mongoue Fanche Aminatou, Fabrice Tchohou Lessa, Gaston Etoga Onana, E. Youmbi
{"title":"Effect of Grafting on Growth and Shelf Life of Tomatoes (<i>Solanum lycopersicum</i> L.) Grafted on Two Local <i>Solanum</i> Species","authors":"Somo Toukam Gabriel Mahbou, Godswill Ntsomboh-Ntsefong, Mongoue Fanche Aminatou, Fabrice Tchohou Lessa, Gaston Etoga Onana, E. Youmbi","doi":"10.4236/abb.2022.139026","DOIUrl":"https://doi.org/10.4236/abb.2022.139026","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/abb.2022.1310030
Gang Yang, Shaojun Yang, Chenbing Wang
{"title":"Study on Analgesia and Sedation of Butorphanol Tartrate Combined with Dexmedetomidine in Severe Cerebral Hemorrhage for Patients with Mechanical Ventilation","authors":"Gang Yang, Shaojun Yang, Chenbing Wang","doi":"10.4236/abb.2022.1310030","DOIUrl":"https://doi.org/10.4236/abb.2022.1310030","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. Hossain, Md. Faizul Hossain Miraz, S. Akter, Md Younus Ali, G. Deb
{"title":"Comparative Study on Semen Quality and Fertility of Red Chittagong Cattle, BCB1 and Munshiganj Bulls of Bangladesh","authors":"S. Hossain, Md. Faizul Hossain Miraz, S. Akter, Md Younus Ali, G. Deb","doi":"10.4236/abb.2022.139028","DOIUrl":"https://doi.org/10.4236/abb.2022.139028","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Antioxidant Properties and Antimicrobial Activity in the Extracts of Two Edible Mushroom, <i>Pleurotus sajor caju</i> and <i>Schizophyllum commune</i>","authors":"Sujjat Al-Azad, Vivian Chong Ai Ping","doi":"10.4236/abb.2022.139023","DOIUrl":"https://doi.org/10.4236/abb.2022.139023","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469525","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/abb.2022.1312036
O. Ibrahim, M. Menkovska
{"title":"Dietary Fibers-Classification, Properties, Analysis and Function: A Review","authors":"O. Ibrahim, M. Menkovska","doi":"10.4236/abb.2022.1312036","DOIUrl":"https://doi.org/10.4236/abb.2022.1312036","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468985","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Al-Baraa Akram, Glen J. P. McCann, Triaziana Sgamma
{"title":"Identification Transgenic DNA of Transformed <i>Arabidopsis</i> Using PCR and Southern Blot","authors":"Al-Baraa Akram, Glen J. P. McCann, Triaziana Sgamma","doi":"10.4236/abb.2022.133006","DOIUrl":"https://doi.org/10.4236/abb.2022.133006","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The quantification of blood/plasma ethanol concentration (BEC/PEC) is of great importance in experiments involving basic research, clinical studies, and bioethanol production. Traditional methods commonly used to measure BEC can be expensive and require high-cost equipment and qualified labor. The aim of this study was to develop a low-cost method that can be performed with simple infrastructure commonly available in research laboratories. For this, we developed a protocol to quantify PEC in mice, using the method of reduction of potassium dichromate by ethanol. However, this oxi-dation-reduction (redox) reaction is not specific to ethanol. Thus, the PEC was measured following a sequence of chemical reactions to eliminate the reductive interfering substances presented in the samples. Firstly, we eva-luated the sensitivity of the dichromate reactive to ethanol and to different reducing substances found in the plasma, in order to determine which the main interfering substances are. Next, once the main interfering substances were determined in the dichromate reduction, plasma was assayed for PEC. First, mice received intraperitoneally (i.p.) saline (basal reading, 0% ethanol) or ethanol injections (0.5, 1, 2, 3, and 4 g/kg) and had their plasma collected. After plasma deproteinization and plasma glucose oxidation, it was mixed with the dichromate/acetic acid reactive, and then the products of the redox reaction were determined by the spectrophotometric method. Then, tivity assay. Therefore, despite the need for a background reading, this method can be successfully applied to determine PEC using low-cost chemical reagents.
{"title":"Spectrophotometric Assay for the Quantification of Plasma Ethanol Levels in Mice through Chromium-Ethanol Oxidation-Reduction Reaction","authors":"M. B. Contó, R. Camarini","doi":"10.4236/abb.2022.134009","DOIUrl":"https://doi.org/10.4236/abb.2022.134009","url":null,"abstract":"The quantification of blood/plasma ethanol concentration (BEC/PEC) is of great importance in experiments involving basic research, clinical studies, and bioethanol production. Traditional methods commonly used to measure BEC can be expensive and require high-cost equipment and qualified labor. The aim of this study was to develop a low-cost method that can be performed with simple infrastructure commonly available in research laboratories. For this, we developed a protocol to quantify PEC in mice, using the method of reduction of potassium dichromate by ethanol. However, this oxi-dation-reduction (redox) reaction is not specific to ethanol. Thus, the PEC was measured following a sequence of chemical reactions to eliminate the reductive interfering substances presented in the samples. Firstly, we eva-luated the sensitivity of the dichromate reactive to ethanol and to different reducing substances found in the plasma, in order to determine which the main interfering substances are. Next, once the main interfering substances were determined in the dichromate reduction, plasma was assayed for PEC. First, mice received intraperitoneally (i.p.) saline (basal reading, 0% ethanol) or ethanol injections (0.5, 1, 2, 3, and 4 g/kg) and had their plasma collected. After plasma deproteinization and plasma glucose oxidation, it was mixed with the dichromate/acetic acid reactive, and then the products of the redox reaction were determined by the spectrophotometric method. Then, tivity assay. Therefore, despite the need for a background reading, this method can be successfully applied to determine PEC using low-cost chemical reagents.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Bénédicte Sophie Adjoua Akakpo, Arnaud Agbidinoukoun, C. Ahanhanzo, Gilles Habib Todjro Cacai, Bienvenu T. Badou, S. Houédjissin, J. Dossou
{"title":"Buds Reactivity and Factors Promoting Shoots Proliferation and Rooting of Cashew Seedlings Using <i>in Vitro</i> Tissue Culture Process","authors":"Bénédicte Sophie Adjoua Akakpo, Arnaud Agbidinoukoun, C. Ahanhanzo, Gilles Habib Todjro Cacai, Bienvenu T. Badou, S. Houédjissin, J. Dossou","doi":"10.4236/abb.2022.139025","DOIUrl":"https://doi.org/10.4236/abb.2022.139025","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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