Pub Date : 2022-01-01DOI: 10.4236/abb.2022.1311033
F. Aşçı, A. Unal, Sultan Demir Şimşek
{"title":"Isolation and Expression Analysis of Meristem Gene <i>CLV</i>2 and Carpel Generation Gene (<i>CRC</i>) from Multi-Carpel Structure of <i>Thermopsis turcica</i>","authors":"F. Aşçı, A. Unal, Sultan Demir Şimşek","doi":"10.4236/abb.2022.1311033","DOIUrl":"https://doi.org/10.4236/abb.2022.1311033","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468838","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Microbial enhanced oil recovery (MEOR) is the research focus in the field of energy development as an environmentally friendly and low cost technology. MEOR can bes divided into indigenous microbial oil recovery and exogenous microbial oil recovery. The ultimate goal of indigenous microbial flooding is to enhance oil recovery via stimulation of specific indigenous microorganisms by injecting optimal nutrients. For studying the specific rule to activate the indigenous community during the long-term injection period, a series of indigenous displacement flooding experiments were carried out by using the long-core physical simulation test. The experimental results have shown that the movement of nutrients components (i.e., carbon/nitrogen/phosphorus) differed from the consumption of them. Moreover, there was a positive relationship between the nutrients concentration and bacteria concentration once observed in the produced fluid. And the trend of concentration of acetic acid was consistent with that of methanogens. When adding same activators, the impacts of selective activators to stimulate the indigenous microorganisms became worse along with the injection period, which led to less oil recovery efficiency.
{"title":"Relationship between Microbial Community Characteristics and Flooding Efficiency in Microbial Enhanced Oil Recovery","authors":"Gangzheng Sun, Jing Hu, Qiong Chen, Zihui Chen, Weidong Wang, Q. Qian, Feng-zhen Han, Ling Li, Yuesheng Li","doi":"10.4236/abb.2022.135014","DOIUrl":"https://doi.org/10.4236/abb.2022.135014","url":null,"abstract":"Microbial enhanced oil recovery (MEOR) is the research focus in the field of energy development as an environmentally friendly and low cost technology. MEOR can bes divided into indigenous microbial oil recovery and exogenous microbial oil recovery. The ultimate goal of indigenous microbial flooding is to enhance oil recovery via stimulation of specific indigenous microorganisms by injecting optimal nutrients. For studying the specific rule to activate the indigenous community during the long-term injection period, a series of indigenous displacement flooding experiments were carried out by using the long-core physical simulation test. The experimental results have shown that the movement of nutrients components (i.e., carbon/nitrogen/phosphorus) differed from the consumption of them. Moreover, there was a positive relationship between the nutrients concentration and bacteria concentration once observed in the produced fluid. And the trend of concentration of acetic acid was consistent with that of methanogens. When adding same activators, the impacts of selective activators to stimulate the indigenous microorganisms became worse along with the injection period, which led to less oil recovery efficiency.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"191 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469055","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The quantification of blood/plasma ethanol concentration (BEC/PEC) is of great importance in experiments involving basic research, clinical studies, and bioethanol production. Traditional methods commonly used to measure BEC can be expensive and require high-cost equipment and qualified labor. The aim of this study was to develop a low-cost method that can be performed with simple infrastructure commonly available in research laboratories. For this, we developed a protocol to quantify PEC in mice, using the method of reduction of potassium dichromate by ethanol. However, this oxi-dation-reduction (redox) reaction is not specific to ethanol. Thus, the PEC was measured following a sequence of chemical reactions to eliminate the reductive interfering substances presented in the samples. Firstly, we eva-luated the sensitivity of the dichromate reactive to ethanol and to different reducing substances found in the plasma, in order to determine which the main interfering substances are. Next, once the main interfering substances were determined in the dichromate reduction, plasma was assayed for PEC. First, mice received intraperitoneally (i.p.) saline (basal reading, 0% ethanol) or ethanol injections (0.5, 1, 2, 3, and 4 g/kg) and had their plasma collected. After plasma deproteinization and plasma glucose oxidation, it was mixed with the dichromate/acetic acid reactive, and then the products of the redox reaction were determined by the spectrophotometric method. Then, tivity assay. Therefore, despite the need for a background reading, this method can be successfully applied to determine PEC using low-cost chemical reagents.
{"title":"Spectrophotometric Assay for the Quantification of Plasma Ethanol Levels in Mice through Chromium-Ethanol Oxidation-Reduction Reaction","authors":"M. B. Contó, R. Camarini","doi":"10.4236/abb.2022.134009","DOIUrl":"https://doi.org/10.4236/abb.2022.134009","url":null,"abstract":"The quantification of blood/plasma ethanol concentration (BEC/PEC) is of great importance in experiments involving basic research, clinical studies, and bioethanol production. Traditional methods commonly used to measure BEC can be expensive and require high-cost equipment and qualified labor. The aim of this study was to develop a low-cost method that can be performed with simple infrastructure commonly available in research laboratories. For this, we developed a protocol to quantify PEC in mice, using the method of reduction of potassium dichromate by ethanol. However, this oxi-dation-reduction (redox) reaction is not specific to ethanol. Thus, the PEC was measured following a sequence of chemical reactions to eliminate the reductive interfering substances presented in the samples. Firstly, we eva-luated the sensitivity of the dichromate reactive to ethanol and to different reducing substances found in the plasma, in order to determine which the main interfering substances are. Next, once the main interfering substances were determined in the dichromate reduction, plasma was assayed for PEC. First, mice received intraperitoneally (i.p.) saline (basal reading, 0% ethanol) or ethanol injections (0.5, 1, 2, 3, and 4 g/kg) and had their plasma collected. After plasma deproteinization and plasma glucose oxidation, it was mixed with the dichromate/acetic acid reactive, and then the products of the redox reaction were determined by the spectrophotometric method. Then, tivity assay. Therefore, despite the need for a background reading, this method can be successfully applied to determine PEC using low-cost chemical reagents.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-01-01DOI: 10.4236/abb.2022.1312037
Qianqian Wang, Ping Wang, Xiaozhen Wang, Xiaowen Wang, Zeyan Zhang, F. Lian, Rong Zhen, Yifei Cao
{"title":"The Activity of AQP9 Is Mediated MAPK in Arsenic-Treated Mouse Model of Hepatocellular Carcinoma","authors":"Qianqian Wang, Ping Wang, Xiaozhen Wang, Xiaowen Wang, Zeyan Zhang, F. Lian, Rong Zhen, Yifei Cao","doi":"10.4236/abb.2022.1312037","DOIUrl":"https://doi.org/10.4236/abb.2022.1312037","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A. Reza, Sabrina Akter Tushi, Nithon Chandra Sahana, Uttom Kumar Bhowmik, A. Majumder, A. Akram
{"title":"Bioequivalence Study of Two Formulations of Telmisartan 40 mg Tablets in Healthy Adult Bangladeshi Subjects under Fasting Conditions","authors":"A. Reza, Sabrina Akter Tushi, Nithon Chandra Sahana, Uttom Kumar Bhowmik, A. Majumder, A. Akram","doi":"10.4236/abb.2022.139027","DOIUrl":"https://doi.org/10.4236/abb.2022.139027","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469140","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Ashley Kelican, Nathan Huysman, J. Voorhees, M. E. Barnes
{"title":"Sutureless Implantation of Acoustic Transmitters in Rainbow Trout Exceeding 2% Tag-to-Body Ratio","authors":"Ashley Kelican, Nathan Huysman, J. Voorhees, M. E. Barnes","doi":"10.4236/abb.2022.136016","DOIUrl":"https://doi.org/10.4236/abb.2022.136016","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468684","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A Review on the Impact of the Plant Bioactive Compound <i>β</i>-Glucans as Phytochemicals for Boosting Human and Animals’ Immune Response—<i>β</i>-Glucans as Immunostimulants","authors":"M. Menkovska, O. Ibrahim","doi":"10.4236/abb.2022.137019","DOIUrl":"https://doi.org/10.4236/abb.2022.137019","url":null,"abstract":"","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70469324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Aml O. Alhadad, Galal S. Salem, M. F. Elmhdwi, Suliman M. Hussein, Sarah M. Elshareef
The current study was carried out to determine the bioactivity of P. lentiscus leaf extracts as potential antibacterial and antioxidant properties. The plant extracts were examined for antibacterial activity against antibiotic-resistant Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, and Proteus mirabilis using the agar well method (according to the guidelines of Clinical and Laboratory Standard Institute). The antioxidant potential of 3 plant leaf extracts was determined by their ability to convert Fe3+ to Fe2+ and scavenge the DPPH free radical. At all concentrations studied, the methanolic leaf extract had higher total phenolic and flavonoid content, as well as stronger antioxidant and antibacterial inhibitory activity compared to aqueous extract. Our findings with P. aeruginosa were especially interesting, because this bacterium was inhibited by methanol extract than that of the reference antibiotics. The results also demonstrated a link between DPPH radical scavenging ability, reducing power, and total phenolic and flavonoid content of plant extracts (r > 0.97, R2 > 0.95, P = 0.01). As a result, the methanolic leaf extract of the chosen plant might be employed as an effective antioxidant and antibacterial agent for the treatment of a variety of morbidities.
本研究旨在确定香菇叶提取物的生物活性及其潜在的抗菌和抗氧化性能。采用琼脂孔法测定植物提取物对耐药金黄色葡萄球菌、溶血葡萄球菌、铜绿假单胞菌和奇异变形杆菌的抑菌活性(按照临床与实验室标准协会的指导方针)。3种植物叶片提取物的抗氧化能力主要取决于其将Fe3+转化为Fe2+和清除DPPH自由基的能力。在所有研究浓度下，与水提物相比，甲醇叶提取物具有更高的总酚和类黄酮含量，以及更强的抗氧化和抗菌抑制活性。我们对铜绿假单胞菌的研究结果特别有趣，因为这种细菌被甲醇提取物抑制，而不是参考抗生素。结果还表明，植物提取物对DPPH自由基的清除能力、还原能力与总酚和总黄酮含量有关(r > 0.97, R2 > 0.95, P = 0.01)。因此，所选植物的甲醇叶提取物可作为一种有效的抗氧化剂和抗菌剂，用于治疗多种疾病。
{"title":"Assessments of Antibacterial and Antioxidant Properties in the Methanolic and Aqueous Leaf Extracts of <i>Pistacia lentiscus</i> against Different Antibiotic Resistance Pathogenic Bacteria","authors":"Aml O. Alhadad, Galal S. Salem, M. F. Elmhdwi, Suliman M. Hussein, Sarah M. Elshareef","doi":"10.4236/abb.2022.133005","DOIUrl":"https://doi.org/10.4236/abb.2022.133005","url":null,"abstract":"The current study was carried out to determine the bioactivity of P. lentiscus leaf extracts as potential antibacterial and antioxidant properties. The plant extracts were examined for antibacterial activity against antibiotic-resistant Staphylococcus aureus, Staphylococcus haemolyticus, Pseudomonas aeruginosa, and Proteus mirabilis using the agar well method (according to the guidelines of Clinical and Laboratory Standard Institute). The antioxidant potential of 3 plant leaf extracts was determined by their ability to convert Fe3+ to Fe2+ and scavenge the DPPH free radical. At all concentrations studied, the methanolic leaf extract had higher total phenolic and flavonoid content, as well as stronger antioxidant and antibacterial inhibitory activity compared to aqueous extract. Our findings with P. aeruginosa were especially interesting, because this bacterium was inhibited by methanol extract than that of the reference antibiotics. The results also demonstrated a link between DPPH radical scavenging ability, reducing power, and total phenolic and flavonoid content of plant extracts (r > 0.97, R2 > 0.95, P = 0.01). As a result, the methanolic leaf extract of the chosen plant might be employed as an effective antioxidant and antibacterial agent for the treatment of a variety of morbidities.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70468746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
I. O. Ogbonna, J. Ikwebe, Otsai Otsima Okpozu, C. Eze, J. Ogbonna
As a way of making algal feedstock feasible for biofuel production, simultaneous utilization of microalga Dictyosphaerium sp. LC172264 for cassava wastewater remediation and accumulation of lipids for biodiesel production was investigated. The algal biomass, lipid contents and composition were measured from the autotrophic, heterotrophic and mixotrophic cultured algal cells. Physicochemical parameters of the cassava wastewater and bioremediation potentials were measured. Biodiesel properties were deduced and compared with the standards. The results showed that mixotrophic culture was the best for both biomass accumulation (1.022 g/L) and lipid contents (24.53%). Irrespective of the culture condition, the predominant fatty acids were similar and included 11-Octadecenoic acid (vaccenic acid (C19H36O2), oleic acid (C18H34O2) and 14-methyl pentadecanoic acid (isopalmitic acid (C17H34O2). The percentage reduction of total dissolved solids was 79.32% and 89.78% for heterotrophy and mixotrophy respectively. Biochemical oxygen demand was 72.95% and 89.35%, chemical oxygen demand was 72.19% and 84.03% whereas cyanide contents reduced from the initial value of 450 mg/L to 93.105 (79.31%) and 85.365 mg/L (81.03%) respectively. Dictyosphaerium sp. showed good growth and lipid production under mixotrophic condition and produced good quality biodiesel under the three cultivation modes. Even though both mixotrophic and heterotrophic conditions had good promise of cassava wastewater remediation by Dictyosphaerium sp., mixotrophy showed superiority.
{"title":"Potential of Dictyosphaerium sp. LC172264 Concomitant Remediation of Cassava Wastewater and Accumulation of Lipids for Biodiesel Production","authors":"I. O. Ogbonna, J. Ikwebe, Otsai Otsima Okpozu, C. Eze, J. Ogbonna","doi":"10.4236/abb.2021.128016","DOIUrl":"https://doi.org/10.4236/abb.2021.128016","url":null,"abstract":"As a way of making algal feedstock feasible for \u0000biofuel production, simultaneous utilization \u0000of microalga Dictyosphaerium sp. \u0000LC172264 for cassava wastewater remediation and accumulation of lipids \u0000for biodiesel production was investigated. The algal biomass, lipid contents \u0000and composition were measured from the autotrophic, heterotrophic and \u0000mixotrophic cultured algal cells. Physicochemical parameters of the cassava \u0000wastewater and bioremediation potentials were measured. Biodiesel properties \u0000were deduced and compared with the standards. The results showed that \u0000mixotrophic culture was the best for both biomass accumulation (1.022 g/L) and \u0000lipid contents (24.53%). Irrespective of the culture condition, the predominant \u0000fatty acids were similar and included 11-Octadecenoic acid (vaccenic acid (C19H36O2), \u0000oleic acid (C18H34O2) and 14-methyl \u0000pentadecanoic acid (isopalmitic acid (C17H34O2). \u0000The percentage reduction of total dissolved solids was 79.32% and 89.78% for \u0000heterotrophy and mixotrophy respectively. Biochemical oxygen demand was 72.95% \u0000and 89.35%, chemical oxygen demand was 72.19% and 84.03% whereas cyanide \u0000contents reduced from the initial value of 450 mg/L to 93.105 (79.31%) and \u000085.365 mg/L (81.03%) respectively. Dictyosphaerium sp. showed good growth and lipid production under mixotrophic condition and \u0000produced good quality biodiesel under the three cultivation modes. Even though \u0000both mixotrophic and heterotrophic conditions had good promise of cassava \u0000wastewater remediation by Dictyosphaerium sp., mixotrophy showed superiority.","PeriodicalId":65405,"journal":{"name":"生命科学与技术进展(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2021-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47684324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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