Hind Baballa Gasmalla, A. Idris, M. I. Shinger, Dongdong Qin, Duoliang Shan, Xiaoquan Lu
Antibacterial activity of iron oxide nanoparticles, an employing B. aegyptiaca oil (L.) Del., was used as natural stabilizer by modifying a co-precipitation method. In this work, we chose B. aegyptiaca oil as the new surfactant coating agent, and synthesized B. aegyptiaca oil coating with iron oxide nanoparticles which were characterized with a variety of methods, including Gas Chromatography (GC) to determine the fatty acids composition of the seeds oil, Fourier Transform-Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) equipped with Energy Dispersive Spectroscopy (EDS), X-ray Powder Diffractometer (XRD) and Vibrating Sample Magnetometer (VSM). In antibacterial studies, disk diffusion susceptibility test was used to measure efficacy of iron oxide nanoparticles against Gram-positive bacteria Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli) in terms of zone inhibition. The B. aegyptiaca coated on the surface of iron oxide nanoparticles; its particle size was found to be nanoscale below 50 nm, and the magnetization (δs) was 16.975 emu g-1. Antibacterial activity was measured. Efficacy of iron oxide nanoparticles against bacterial strains was found in Escherichia coli (E. coli). All these findings suggest that the nanoparticles synthesized from B. aegyptiaca oil may be a promising reagent for a wide variety of applications in biological fields as well as in nanomedicine.
{"title":"Balanites aegyptiaca Oil Synthesized Iron Oxide Nanoparticles: Characterization and Antibacterial Activity","authors":"Hind Baballa Gasmalla, A. Idris, M. I. Shinger, Dongdong Qin, Duoliang Shan, Xiaoquan Lu","doi":"10.4236/JBNB.2016.73016","DOIUrl":"https://doi.org/10.4236/JBNB.2016.73016","url":null,"abstract":"Antibacterial activity of iron oxide nanoparticles, an employing B. aegyptiaca oil (L.) Del., was used as natural stabilizer by modifying a co-precipitation method. In this work, we chose B. aegyptiaca oil as the new surfactant coating agent, and synthesized B. aegyptiaca oil coating with iron oxide nanoparticles which were characterized with a variety of methods, including Gas Chromatography (GC) to determine the fatty acids composition of the seeds oil, Fourier Transform-Infrared Spectroscopy (FTIR), Transmission Electron Microscopy (TEM) equipped with Energy Dispersive Spectroscopy (EDS), X-ray Powder Diffractometer (XRD) and Vibrating Sample Magnetometer (VSM). In antibacterial studies, disk diffusion susceptibility test was used to measure efficacy of iron oxide nanoparticles against Gram-positive bacteria Staphylococcus aureus (S. aureus), Bacillus subtilis (B. subtilis) and Gram-negative bacteria Escherichia coli (E. coli) in terms of zone inhibition. The B. aegyptiaca coated on the surface of iron oxide nanoparticles; its particle size was found to be nanoscale below 50 nm, and the magnetization (δs) was 16.975 emu g-1. Antibacterial activity was measured. Efficacy of iron oxide nanoparticles against bacterial strains was found in Escherichia coli (E. coli). All these findings suggest that the nanoparticles synthesized from B. aegyptiaca oil may be a promising reagent for a wide variety of applications in biological fields as well as in nanomedicine.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"154-165"},"PeriodicalIF":0.0,"publicationDate":"2016-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Marie Siampiringue, C. Massard, E. Caudron, Y. Sibaud, M. Sarakha, K. Awitor
In this work, we study the influence of the annealing treatment on the behaviour of titanium dioxide nanotube layers. The heat treatment protocol is actually the key parameter to induce stable oxide layers and needs to be better understood. Nanotube layers were prepared by electrochemical anodization of Ti foil in 0.4 wt% hydrofluoric acid solution during 20 minutes and then annealed in air atmosphere. In-situ X-ray diffraction analysis, coupled with thermogravimetry, gives us an inside on the oxidation behaviour of titanium dioxide nanotube layers compared to bulk reference samples. Structural studies were performed at 700°C for 12 h in order to follow the time consequences on the oxidation of the material, in sufficient stability conditions. In-situ XRD brought to light that the amorphous oxide layer induced by anodization is responsible for the simultaneous growths of anatase and rutile phase during the first 30 minutes of annealing while the bulk sample oxidation leads to the nucleation of a small amount of anatase TiO2. The initial amorphous oxide layer created by anodization is also responsible for the delay in crystallization compared to the bulk sample. Thermogravimetric analysis exhibits parabolic shape of the mass gain for both anodized and bulk sample; this kinetics is caused by the formation of a rutile external protective layer, as depicted by the associated in-situ XRD diffractograms. We recorded that titanium dioxide nanotube layers exhibit a lower mean mass gain than the bulk, because of the presence of an initial amorphous oxide layer on anodized samples. In-situ XRD results also provide accurate information concerning the sub-layers behavior during the annealing treatment for the bulk and nanostructured layer. Anatase crystallites are mainly localized at the interface oxide layer-metal and the rutile is at the external interface. Sample surface topography was characterized using scanning electron microscopy (SEM). As a probe of the photoactivity of the annealed TiO2 nanotube layers, degradation of an acid orange 7 (AO7) dye solution and 4-chlorophenol under UV irradiation (at 365 nm) were performed. Such titanium dioxide nanotube layers show an efficient photocatalytic activity and the analytical results confirm the degradation mechanism of the 4-chlorophenol reported elsewhere.
{"title":"Impact of Annealing Treatment on the Behaviour of Titanium Dioxide Nanotube Layers","authors":"Marie Siampiringue, C. Massard, E. Caudron, Y. Sibaud, M. Sarakha, K. Awitor","doi":"10.4236/JBNB.2016.73015","DOIUrl":"https://doi.org/10.4236/JBNB.2016.73015","url":null,"abstract":"In this work, we study \u0000the influence of the annealing treatment on the behaviour of titanium dioxide \u0000nanotube layers. The heat treatment protocol is actually the key parameter to \u0000induce stable oxide layers and needs to be better understood. Nanotube layers \u0000were prepared by electrochemical anodization of Ti foil in 0.4 wt% hydrofluoric \u0000acid solution during 20 minutes and then annealed in air atmosphere. In-situ X-ray diffraction analysis, \u0000coupled with thermogravimetry, gives us an inside on the oxidation behaviour of \u0000titanium dioxide nanotube layers compared to bulk reference samples. Structural \u0000studies were performed at 700°C for 12 h in order to follow the time \u0000consequences on the oxidation of the material, in sufficient stability \u0000conditions. In-situ XRD brought to light that the amorphous oxide layer induced by \u0000anodization is responsible for the simultaneous growths of anatase and rutile phase \u0000during the first 30 minutes of annealing while the bulk sample oxidation leads \u0000to the nucleation of a small amount of anatase TiO2. The initial \u0000amorphous oxide layer created by anodization is also responsible for the delay \u0000in crystallization compared to the bulk sample. Thermogravimetric analysis \u0000exhibits parabolic shape of the mass gain for both anodized and bulk sample; this \u0000kinetics is caused by the formation of a rutile external protective layer, as \u0000depicted by the associated in-situ XRD \u0000diffractograms. We recorded that titanium dioxide nanotube layers exhibit a \u0000lower mean mass gain than the bulk, because of the presence of an initial \u0000amorphous oxide layer on anodized samples. In-situ XRD results also provide accurate information concerning the sub-layers \u0000behavior during the annealing treatment for the bulk and nanostructured layer. Anatase \u0000crystallites are mainly localized at the interface oxide layer-metal and the \u0000rutile is at the external interface. Sample surface topography was characterized \u0000using scanning electron microscopy (SEM). As a probe of the photoactivity of \u0000the annealed TiO2 nanotube layers, degradation of an acid orange 7 \u0000(AO7) dye solution and 4-chlorophenol under UV irradiation (at 365 nm) were \u0000performed. Such titanium dioxide nanotube layers show an efficient \u0000photocatalytic activity and the analytical results confirm the degradation \u0000mechanism of the 4-chlorophenol reported elsewhere.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"142-153"},"PeriodicalIF":0.0,"publicationDate":"2016-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biodegradable polymeric materials are the most common carriers for use in drug delivery systems. With this trend, newer drug delivery systems using targeted and controlled release polymeric nanoparticles (NPs) are being developed to manipulate their navigation in complex in vivo environment. However, a clear understanding of the interactions between biological systems and these nanoparticulates is still unexplored. Different studies have been performed to correlate the physicochemical properties of polymeric NPs with the biological responses. Size and surface charge are the two fundamental physicochemical properties that provide a key direction to design an effective NP formulation. In this critical review, our goal is to provide a brief overview on the influences of size and surface charge of different polymeric NPs in vitro and to highlight the challenges involved with in vivo trials.
{"title":"Effects of Size and Surface Charge of Polymeric Nanoparticles on in Vitro and in Vivo Applications","authors":"Sams M A Sadat, S. Jahan, A. Haddadi","doi":"10.4236/JBNB.2016.72011","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72011","url":null,"abstract":"Biodegradable polymeric materials are the most common carriers for use in drug delivery systems. With this trend, newer drug delivery systems using targeted and controlled release polymeric nanoparticles (NPs) are being developed to manipulate their navigation in complex in vivo environment. However, a clear understanding of the interactions between biological systems and these nanoparticulates is still unexplored. Different studies have been performed to correlate the physicochemical properties of polymeric NPs with the biological responses. Size and surface charge are the two fundamental physicochemical properties that provide a key direction to design an effective NP formulation. In this critical review, our goal is to provide a brief overview on the influences of size and surface charge of different polymeric NPs in vitro and to highlight the challenges involved with in vivo trials.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"91-108"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896362","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pouya Moghimian, V. Srot, B. Pichon, S. Facey, P. Aken
The effect of different types of organic solvents on the structural integrity of M13 phages has been directly visualized by transmission and scanning electron microscopy. The exposure of M13 phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm diameter upon exposure to polar organic solvents. We show that this finding can be beneficial for the macromolecular self-assembly and in broader aspects, to enhance the spatial arrangement of desired inorganic nanoparticles in the rapidly developing field of virotronics.
{"title":"Stability of M13 Phage in Organic Solvents","authors":"Pouya Moghimian, V. Srot, B. Pichon, S. Facey, P. Aken","doi":"10.4236/JBNB.2016.72009","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72009","url":null,"abstract":"The effect of different types of organic solvents on the structural integrity of M13 phages has been \u0000directly visualized by transmission and scanning electron microscopy. The exposure of M13 \u0000phages to apolar hexane had no effect on the structure of the phages for up to 8 h. In contrast, \u0000phages showed ~10-fold contraction into rod-like I-forms and to flattened spheroids with ~12 nm \u0000diameter upon exposure to polar organic solvents. We show that this finding can be beneficial for \u0000the macromolecular self-assembly and in broader aspects, to enhance the spatial arrangement of \u0000desired inorganic nanoparticles in the rapidly developing field of virotronics.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"72-77"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896017","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Katrin Quester, M. Avalos-Borja, E. Castro-Longoria
The search for reliable and eco-friendly methods for the production of nanoscale materials is an important aspect of nanotechnology. Silver nanoparticles (AgNPs) are of special interest because of their antimicrobial properties, especially those of small size. In this work, AgNPs were produced under different conditions of temperature and pH using the extract from the fungus Neurospora crassa as reducing agent. Mainly quasi-spherical particles were obtained at all incubation conditions. However, optimum conditions to produce small sizes in the range of 2 - 9 nm were at 4°C and pH 3, also particles of 2 - 22 nm were obtained at 25°C with unmodified pH (6.5) and pH 10. Nevertheless, only particles synthesized at 25°C and pH 10 maintained the same size range after storage of 10 months. In summary, optimal incubation conditions for the synthesis of silver nanoparticles of small size range are reported. This improves the storage time of particles without losing its original size and without going into aggregation or agglomeration.
{"title":"Controllable Biosynthesis of Small Silver Nanoparticles Using Fungal Extract","authors":"Katrin Quester, M. Avalos-Borja, E. Castro-Longoria","doi":"10.4236/JBNB.2016.72013","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72013","url":null,"abstract":"The search for reliable and eco-friendly methods for the production of nanoscale materials is an \u0000important aspect of nanotechnology. Silver nanoparticles (AgNPs) are of special interest because \u0000of their antimicrobial properties, especially those of small size. In this work, AgNPs were produced \u0000under different conditions of temperature and pH using the extract from the fungus Neurospora \u0000crassa as reducing agent. Mainly quasi-spherical particles were obtained at all incubation conditions. \u0000However, optimum conditions to produce small sizes in the range of 2 - 9 nm were at 4°C and \u0000pH 3, also particles of 2 - 22 nm were obtained at 25°C with unmodified pH (6.5) and pH 10. Nevertheless, \u0000only particles synthesized at 25°C and pH 10 maintained the same size range after storage \u0000of 10 months. In summary, optimal incubation conditions for the synthesis of silver nanoparticles \u0000of small size range are reported. This improves the storage time of particles without losing \u0000its original size and without going into aggregation or agglomeration.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"129 10 1","pages":"118-125"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Morphological characteristics and proximate chemical analysis of E. binata showed it as a suitable raw material for writing and printing grade paper. Maximum pulp yield (43.58%) of kappa number 17.38 with 0.9% screening rejects was obtained at 12% of active alkali (as Na2O) pulping temperature 130°C, cooking time 120 min and solid to liquor ratio 1:4. During bio-soda pulping of E. binata, pulp yield decreased slightly while pulp brightness, tensile index, burst index and double fold numbers were improved by 4.1%, 24.94%, 14.03% and 48.45% respectively compared to soda pulping. During ethanol-soda pulping of E. binata, pulp yield, pulp brightness, tensile index, burst index and double fold numbers were improved by 3.9%, 6.6%, 32.18%, 35.40% and 77.31% respectively compared to soda pulping.
{"title":"Effects of Ethanol Addition and Biological Pretreatment on Soda Pulping of Eulaliopsis binata","authors":"A. Gautam, Amit Kumar, D. Dutt","doi":"10.4236/JBNB.2016.72010","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72010","url":null,"abstract":"Morphological characteristics and proximate chemical analysis of E. binata showed it as a suitable \u0000raw material for writing and printing grade paper. Maximum pulp yield (43.58%) of kappa number \u000017.38 with 0.9% screening rejects was obtained at 12% of active alkali (as Na2O) pulping \u0000temperature 130°C, cooking time 120 min and solid to liquor ratio 1:4. During bio-soda pulping of E. binata, pulp yield decreased slightly while pulp brightness, tensile index, burst index and double \u0000fold numbers were improved by 4.1%, 24.94%, 14.03% and 48.45% respectively compared to soda \u0000pulping. During ethanol-soda pulping of E. binata, pulp yield, pulp brightness, tensile index, \u0000burst index and double fold numbers were improved by 3.9%, 6.6%, 32.18%, 35.40% and 77.31% \u0000respectively compared to soda pulping.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"78-90"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896114","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As a major plasma protein, albumin has a distinct advantage compared with other materials for nanoparticle preparation. It is cheap and easily available. The present work aimed to prepare bovine albumin nanoparticles (BAN) with a simple coacervation method and to test their hemocompatibility. The albumin nanoparticles obtained by this method had a range of sizes from 250 - 350 nm at pH = 7.4. In vitro hemocompatibility tests of the prepared (BAN) were conducted after the incubation of BAN with normal blood for 2 h at 37°C. Hemocompatibility tests showed that the reduction in the hemolysis percentage of erythrocytes was due to exposure to BAN. The other blood parameters such as hemoglobin (HG), mean corpuscle hemoglobin (MCH), and mean corpuscle hemoglobin concentration (MCHC) were in the normal range. The prothrombin time (PT) and erythrocyte sedimentation rate (ESR) decreased as the concentration of BAN increased. The results obtained in this study demonstrated that BAN could be used safely and without abnormal effect when interacted with blood through many biomedical applications.
{"title":"Hemocompatibility of Albumin Nanoparticles as a Drug Delivery System—An in Vitro Study","authors":"M. A. Elblbesy","doi":"10.4236/JBNB.2016.72008","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72008","url":null,"abstract":"As a major plasma protein, albumin has a distinct advantage compared with other materials for \u0000nanoparticle preparation. It is cheap and easily available. The present work aimed to prepare bovine \u0000albumin nanoparticles (BAN) with a simple coacervation method and to test their hemocompatibility. \u0000The albumin nanoparticles obtained by this method had a range of sizes from 250 - 350 \u0000nm at pH = 7.4. In vitro hemocompatibility tests of the prepared (BAN) were conducted after the \u0000incubation of BAN with normal blood for 2 h at 37°C. Hemocompatibility tests showed that the reduction \u0000in the hemolysis percentage of erythrocytes was due to exposure to BAN. The other blood \u0000parameters such as hemoglobin (HG), mean corpuscle hemoglobin (MCH), and mean corpuscle \u0000hemoglobin concentration (MCHC) were in the normal range. The prothrombin time (PT) and \u0000erythrocyte sedimentation rate (ESR) decreased as the concentration of BAN increased. The results \u0000obtained in this study demonstrated that BAN could be used safely and without abnormal effect \u0000when interacted with blood through many biomedical applications.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"64-71"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
K. Han, C. Takagi, Chunwei Wu, H. Mizukami, A. Ostafin
Perfluorocarbon emulsion has been studied as an oxygen carrier, due to its high oxygen content. In clinical trials, it has shown stability in delivering oxygen to the target region. The purpose of the present study was to increase the stability of the emulsion by coating its surface with calcium phosphate. A layer-by-layer method was employed to coat the flexible emulsion surface. Considering the ionic affinity of calcium and phosphate to the lecithin emulsion surface, the first layer of coating was calcium and the second layer was comprised of phosphate ion. The coated emulsion demonstrated various oxygen release times depending on the thickness of the layers: from 0.04 sec. for a thickness of 8 nm to 0.17 sec. for a thickness 38 nm. Overall, the stability of the calcium phosphate coated emulsion was increased, while its original function as an oxygen carrier was maintained.
{"title":"Synthesis of Calcium Phosphate Controllable Coating Thickness on Oil-in-Water Nanoemulsion with Performance of Oxygen Release as Oxygen Carrier","authors":"K. Han, C. Takagi, Chunwei Wu, H. Mizukami, A. Ostafin","doi":"10.4236/JBNB.2016.72007","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72007","url":null,"abstract":"Perfluorocarbon emulsion has been studied as an oxygen carrier, due to its high oxygen content. In clinical trials, it has shown stability in delivering oxygen to the target region. The purpose of the present study was to increase the stability of the emulsion by coating its surface with calcium phosphate. A layer-by-layer method was employed to coat the flexible emulsion surface. Considering the ionic affinity of calcium and phosphate to the lecithin emulsion surface, the first layer of coating was calcium and the second layer was comprised of phosphate ion. The coated emulsion demonstrated various oxygen release times depending on the thickness of the layers: from 0.04 sec. for a thickness of 8 nm to 0.17 sec. for a thickness 38 nm. Overall, the stability of the calcium phosphate coated emulsion was increased, while its original function as an oxygen carrier was maintained.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"55-63"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
S. A. Mualla, R. Farahat, P. Basmaji, G. Olyveira, L. Costa, José C Oliveira, Gino Bruno Francozo
Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. In addition, biomedical materials have claimed attention because of the increased interest in tissue engineering materials for wound care and regenerative medicine. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin®) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified before the bacteria are inoculated for mimicking ECM to cells support and built new local material for wound healing. Chemical groups influences and thermal behavior in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Besides, In vivo analysis was evaluated with clinical study at Sharjah Kuwait Hospital.
{"title":"Study of Nanoskin ECM-Bacterial Cellulose Wound Healing/United Arab Emirates","authors":"S. A. Mualla, R. Farahat, P. Basmaji, G. Olyveira, L. Costa, José C Oliveira, Gino Bruno Francozo","doi":"10.4236/JBNB.2016.72012","DOIUrl":"https://doi.org/10.4236/JBNB.2016.72012","url":null,"abstract":"Natural extracellular matrices (ECMs) perform the tasks necessary for tissue formation, maintenance, regulation and function, providing a powerful means of controlling the biological performance of regenerative materials. In addition, biomedical materials have claimed attention because of the increased interest in tissue engineering materials for wound care and regenerative medicine. Moreover, the nanostructure and morphological similarities with collagen make BC attractive for cell immobilization, cell support and Natural Extracellular Matrix (ECM) Scaffolds. In this work, we present the extracellular matrix (ECM) using the bacterial cellulose (Nanoskin®) which regulates cell behavior by influencing cell proliferation, survival, shape, migration and differentiation. Bacterial cellulose fermentation process is modified before the bacteria are inoculated for mimicking ECM to cells support and built new local material for wound healing. Chemical groups influences and thermal behavior in bacterial cellulose were analyzed using transmission infrared spectroscopy (FTIR) and thermogravimetric analysis (TGA), respectively. Besides, In vivo analysis was evaluated with clinical study at Sharjah Kuwait Hospital.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"109-117"},"PeriodicalIF":0.0,"publicationDate":"2016-03-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Chenghu Liu, L. Hou, Li‐li Liu, Z. Qu, Xin Wang, Xiaoxia Sun, Ping Wu, Yanping Shi
In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC50 values among different dilutions of AgNO3 solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC50 values of AgNO50 solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC50 value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.
{"title":"In Vitro Cytotoxicity Evaluation of Highly Absorbent Foam Dressings Based on Silver Zirconium Phosphate via IC50 Value","authors":"Chenghu Liu, L. Hou, Li‐li Liu, Z. Qu, Xin Wang, Xiaoxia Sun, Ping Wu, Yanping Shi","doi":"10.4236/JBNB.2016.71005","DOIUrl":"https://doi.org/10.4236/JBNB.2016.71005","url":null,"abstract":"In this study, we, for the first time, tried to apply IC50 values (inhibitory concentration estimated to affect the endpoint in question by 50%) in the MTT colorimetric assay to investigate the cytotoxic effects of highly absorbent foam dressings based on silver zirconium phosphate, a newly nano-based matrix. Our results showed that silver released from dressings based on silver zirconium phosphate attributed mainly to highly cytotoxic to L929 cells cultured with MEM containing 10% fetal bovine serum. In addition, we have also compared the IC50 values among different dilutions of AgNO3 solution, silver based dressing extracts and material reference control (ZDEC) extracts using the optimized MTT assay, along with characterizing the silver content in the dressing extracts using atomic absorption spectroscopy. Results have shown that the IC50 values of AgNO50 solution, silver based dressing extracts and ZDEC extracts are 3.5 μg/mL, 3.8 μg/mL and 8.4%, respectively. And there exist some good agreements between qualitative and quantitative evaluation method as well. In conclusion, our study has led to the view that the IC50 value is a promising quantitative index for screening cytotoxicity with regard to silver based dressings.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"07 1","pages":"37-44"},"PeriodicalIF":0.0,"publicationDate":"2016-01-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70895547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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