Pub Date : 2020-05-11DOI: 10.4236/jbnb.2020.113010
T. A. Marinho, Patrícia Silva Nunes, P. R. Melo-Reis, M. R. F. Machado, Nelson Jorge da Silva Júnior
The SONE oil/water was prepared using the pseudo-ternary phase diagram, using a low energy method with phase inversion by changing the composition. In order to optimize the preparation of SONE, three speeds were used in the agitator arm and two different rods. The particle size and polydispersity index were determined by Dynamic Light Scattering (DLS) and the stability test by the freeze-thaw cycle. Angiogenesis in chicken embryo egg chorioallantoic membrane and zebrafish (Danio rerio) embryotoxicity was performed. The lower stirring speed and propeller shaft induced smaller particle size (550.2 nm). Regarding angiogenesis, there was a statistically significant difference for all the evaluated parameters (length, caliber, junctions, and number of blood vessel complexes) and the result was higher in SONE when compared to the inhibition control (Dexamethasone), but lower than the induction control (Regederm®) and there was no statistically significant difference between SONE and distilled water. It was observed that the exposure of the zebrafish embryos to SONE caused an increase in the mortality rate dependent on time and concentration. The LC50 for SONE decreased statistically with increasing exposure (p-value = 0.046). Heart rate decreased significantly with increasing concentration at all exposure times (p-value < 0.05), as a result of progressive embryo mortality. The hatching rate was late until the concentration of 0.0193 mg/mL and no hatching rate was verified from that concentration. Exposure of zebrafish embryos to different concentrations of SONE induced malformations such as spinal changes, pericardial edema and yolk sac edema, but there was no significant difference in the malformation rate of embryos exposed to SONE when compared to the control group. The SONE produced remained stable in the freeze-thaw cycle, with changes only in pH. Despite the low results for embryotoxicity, further studies are needed, aiming at the ideal formulation for angiogenesis purposes.
{"title":"Production of Soybean Oil Nanoemulsion (SONE) and Evaluation of Angiogenic and Embryotoxic Activity","authors":"T. A. Marinho, Patrícia Silva Nunes, P. R. Melo-Reis, M. R. F. Machado, Nelson Jorge da Silva Júnior","doi":"10.4236/jbnb.2020.113010","DOIUrl":"https://doi.org/10.4236/jbnb.2020.113010","url":null,"abstract":"The SONE oil/water was prepared using the pseudo-ternary phase diagram, using a low energy method with phase inversion by changing the composition. In order to optimize the preparation of SONE, three speeds were used in the agitator arm and two different rods. The particle size and polydispersity index were determined by Dynamic Light Scattering (DLS) and the stability test by the freeze-thaw cycle. Angiogenesis in chicken embryo egg chorioallantoic membrane and zebrafish (Danio rerio) embryotoxicity was performed. The lower stirring speed and propeller shaft induced smaller particle size (550.2 nm). Regarding angiogenesis, there was a statistically significant difference for all the evaluated parameters (length, caliber, junctions, and number of blood vessel complexes) and the result was higher in SONE when compared to the inhibition control (Dexamethasone), but lower than the induction control (Regederm®) and there was no statistically significant difference between SONE and distilled water. It was observed that the exposure of the zebrafish embryos to SONE caused an increase in the mortality rate dependent on time and concentration. The LC50 for SONE decreased statistically with increasing exposure (p-value = 0.046). Heart rate decreased significantly with increasing concentration at all exposure times (p-value < 0.05), as a result of progressive embryo mortality. The hatching rate was late until the concentration of 0.0193 mg/mL and no hatching rate was verified from that concentration. Exposure of zebrafish embryos to different concentrations of SONE induced malformations such as spinal changes, pericardial edema and yolk sac edema, but there was no significant difference in the malformation rate of embryos exposed to SONE when compared to the control group. The SONE produced remained stable in the freeze-thaw cycle, with changes only in pH. Despite the low results for embryotoxicity, further studies are needed, aiming at the ideal formulation for angiogenesis purposes.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42755611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-05-11DOI: 10.4236/jbnb.2020.113011
P. Basmaji
It’s well known that the cancer cell has tendency to grow fast. Chemotherapy drugs have been used in order to kill cancer growing cells and take immune system weakly. However, side effect can damage these healthy cells. Moreover, it is not natural treatment. Natural alternative cancer treatments may be able to help and open new way for cancer treatment. In this work, we transfer cancer nodule to wound and we treat the nodule as wound, using Nanoskin® advance cell therapy (ACT), natural extra cellular matrix which releases oxygen to the cancer tissue. Our result shows that the cancer nodule becomes like chronic wound opened and then disappeared. In addition, we obtained complete healing wound.
{"title":"First Breast Cancer Treatment Naturally by Nanoskin Act","authors":"P. Basmaji","doi":"10.4236/jbnb.2020.113011","DOIUrl":"https://doi.org/10.4236/jbnb.2020.113011","url":null,"abstract":"It’s well known that the cancer cell has tendency to grow fast. Chemotherapy drugs have been used in order to kill cancer growing cells and take immune system weakly. However, side effect can damage these healthy cells. Moreover, it is not natural treatment. Natural alternative cancer treatments may be able to help and open new way for cancer treatment. In this work, we transfer cancer nodule to wound and we treat the nodule as wound, using Nanoskin® advance cell therapy (ACT), natural extra cellular matrix which releases oxygen to the cancer tissue. Our result shows that the cancer nodule becomes like chronic wound opened and then disappeared. In addition, we obtained complete healing wound.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-05-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49377840","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-04DOI: 10.4236/jbnb.2020.112008
Mengru Jin, Q. Xing, Zikang Chen
As the mimic biology becomes more and more important in the field of technology, superhydrophobic materials in the natural world have also become common. Superhydrophobic surfaces are used to prevent water droplets from wetting themselves which contain the micro- and nano-structures named hierarchical surfaces and exhibit the high water contact angles (WCA) that are greater than 150˚ and perfect application foreground in both our daily lives and industry. In this work, we first discuss several surface properties and their numerical models. And then we list the surface properties of a variety of natural superhydrophobic surfaces and sum up their similarities and differences. The most recent strategies of how to apply natural superhydrophobic surfaces are also introduced within the past several years. In addition, we talk about the limitations of the current generation of superhydrophobic surfaces and prospects which looks for solutions to the problems. This review aims to enable researchers to learn more about the principles and mechanisms of superhydrophobicity and perceive the new methods for creating and modifying it.
{"title":"A Review: Natural Superhydrophobic Surfaces and Applications","authors":"Mengru Jin, Q. Xing, Zikang Chen","doi":"10.4236/jbnb.2020.112008","DOIUrl":"https://doi.org/10.4236/jbnb.2020.112008","url":null,"abstract":"As the mimic biology becomes more and more important in the field of technology, superhydrophobic materials in the natural world have also become common. Superhydrophobic surfaces are used to prevent water droplets from wetting themselves which contain the micro- and nano-structures named hierarchical surfaces and exhibit the high water contact angles (WCA) that are greater than 150˚ and perfect application foreground in both our daily lives and industry. In this work, we first discuss several surface properties and their numerical models. And then we list the surface properties of a variety of natural superhydrophobic surfaces and sum up their similarities and differences. The most recent strategies of how to apply natural superhydrophobic surfaces are also introduced within the past several years. In addition, we talk about the limitations of the current generation of superhydrophobic surfaces and prospects which looks for solutions to the problems. This review aims to enable researchers to learn more about the principles and mechanisms of superhydrophobicity and perceive the new methods for creating and modifying it.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"11 1","pages":"110-149"},"PeriodicalIF":0.0,"publicationDate":"2020-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46094900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-04DOI: 10.4236/jbnb.2020.112006
Manjushree Bahir, R. Khairnar, M. Mahabole
The application of electric field to graft materials has significant contribution in bone healing mechanism. Hence, the aim of this study is to develop conductive hydroxyapatite (HAp) scaffolds by introducing different concentrations of silver ion into its structure and demonstrate its impact on in vitro bioactivity and electrical properties. Hydroxyapatite was synthesized by wet chemical method and calcium ions from HAp structure have been partially replaced by silver ions. The HAp and Ag-HAp nanocomposites were characterized by Fourier-transform infrared, Raman spectroscopy, XRD and EDAX for functional group and phase formation analysis as well as to confirm existence of silver ions in HAp structure respectively. Bioactivity of these scaffolds was assessed by using simulated body fluid. The surface morphology, structural analysis and electrical properties of scaffolds before and after formation of newly calcified tissues on its surface were examined via scanning electron microscopy (SEM), XRD, FTIR, dielectric and impedance spectroscopy techniques. Overall, our finding suggests that the administration of silver ions in HAp scaffold boosts bioactivity and has strong correlation with electrical properties.
{"title":"Electrical Properties of Newly Calcified Tissues on the Surface of Silver Ion Administrated Hydroxyapatite Scaffolds","authors":"Manjushree Bahir, R. Khairnar, M. Mahabole","doi":"10.4236/jbnb.2020.112006","DOIUrl":"https://doi.org/10.4236/jbnb.2020.112006","url":null,"abstract":"The application of electric field to graft materials has significant contribution in bone healing mechanism. Hence, the aim of this study is to develop conductive hydroxyapatite (HAp) scaffolds by introducing different concentrations of silver ion into its structure and demonstrate its impact on in vitro bioactivity and electrical properties. Hydroxyapatite was synthesized by wet chemical method and calcium ions from HAp structure have been partially replaced by silver ions. The HAp and Ag-HAp nanocomposites were characterized by Fourier-transform infrared, Raman spectroscopy, XRD and EDAX for functional group and phase formation analysis as well as to confirm existence of silver ions in HAp structure respectively. Bioactivity of these scaffolds was assessed by using simulated body fluid. The surface morphology, structural analysis and electrical properties of scaffolds before and after formation of newly calcified tissues on its surface were examined via scanning electron microscopy (SEM), XRD, FTIR, dielectric and impedance spectroscopy techniques. Overall, our finding suggests that the administration of silver ions in HAp scaffold boosts bioactivity and has strong correlation with electrical properties.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"11 1","pages":"83-100"},"PeriodicalIF":0.0,"publicationDate":"2020-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49403268","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-02-04DOI: 10.4236/jbnb.2020.112007
C. Mattos, M. A. Carvalho, J. J. Carvalho, P. Santos, J. Pires, I. Brum
Background: Narrow-diameter implants (3.0 - 3.5 mm range) have been introduced for the replacement of teeth with insufficient bone structure and/or limited mesiodistal or interimplant spaces, and appear to offer clinical results similar to those obtained with implants of greater diameter. Studies using extra-narrow-diameter implants (2.8 mm) are scarce. Case Presentation: A 59-year-old male patient received two extra-narrow-diameter implants, 2.8 × 11 mm in the region between elements 11 and 14. Together, two 3.5 × 8.5 mm SYSTHEX® platform 4.1 implants were installed in the region of elements 15 and 16 to provide greater stability in the occlusion. Of four previous implants on the maxillary left side, one in the region between the elements 23 and 24 that was located in a very apical position and vestibularized was removed. The provisional was already installed on the elements 11, 21, and 22 with the metal cores already prepared and with the Globteck® implants in the region of the elements 23, 24, and 27. The functional and esthetic results were satisfactory. Conclusions: Insertion of extra-narrow-diameter implants of 2.8 mm in the maxillary anterior region is a reliable option in a patient with absence of elements 12 and 13, restoring masticatory function and aesthetics in the upper arch.
背景：窄直径种植体（3.0-3.5 mm范围）已被引入用于替换骨结构不足和/或近中或种植间隙有限的牙齿，并且似乎提供了与较大直径种植体相似的临床结果。使用超窄直径植入物（2.8毫米）的研究很少。病例介绍：一名59岁男性患者接受了两个超窄直径植入物，在元件11和14之间的区域为2.8×11 mm。两个3.5×8.5 mm SYSTHEX®平台4.1植入物一起安装在元件15和16的区域，以提供更大的咬合稳定性。在上颌左侧先前的四个植入物中，位于元件23和24之间的区域中的一个位于非常顶端的位置并且前庭化的植入物被移除。临时支架已经安装在元件11、21和22上，金属芯已经准备好，Globtec®植入物位于元件23、24和27的区域。功能和美观效果令人满意。结论：对于没有元件12和13的患者，在上颌前部区域插入2.8 mm的超窄直径植入物是一种可靠的选择，可以恢复上弓的咀嚼功能和美观。
{"title":"Use of Extra-Narrow-Diameter Implants in Reduced Alveolar Ridge: A Case Report","authors":"C. Mattos, M. A. Carvalho, J. J. Carvalho, P. Santos, J. Pires, I. Brum","doi":"10.4236/jbnb.2020.112007","DOIUrl":"https://doi.org/10.4236/jbnb.2020.112007","url":null,"abstract":"Background: Narrow-diameter implants (3.0 - 3.5 mm range) have been introduced for the replacement of teeth with insufficient bone structure and/or limited mesiodistal or interimplant spaces, and appear to offer clinical results similar to those obtained with implants of greater diameter. Studies using extra-narrow-diameter implants (2.8 mm) are scarce. Case Presentation: A 59-year-old male patient received two extra-narrow-diameter implants, 2.8 × 11 mm in the region between elements 11 and 14. Together, two 3.5 × 8.5 mm SYSTHEX® platform 4.1 implants were installed in the region of elements 15 and 16 to provide greater stability in the occlusion. Of four previous implants on the maxillary left side, one in the region between the elements 23 and 24 that was located in a very apical position and vestibularized was removed. The provisional was already installed on the elements 11, 21, and 22 with the metal cores already prepared and with the Globteck® implants in the region of the elements 23, 24, and 27. The functional and esthetic results were satisfactory. Conclusions: Insertion of extra-narrow-diameter implants of 2.8 mm in the maxillary anterior region is a reliable option in a patient with absence of elements 12 and 13, restoring masticatory function and aesthetics in the upper arch.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41627109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-14DOI: 10.4236/jbnb.2020.111005
Hazim S. Aljewari, MT Romo Castro, Olivia Solomon, Quincy C. Moore, F. Nave, A. Thompson
This study investigates poly(vinyl alcohol) (PVA) membranes as controlled release micro-matrices, which can be useful in therapeutic applications for optimizing the administration of drugs. Currently, the use of hydrogels is limited by protein size. This study investigates the delivery of PspA, a large protein of approximately 38 kD. Pneumococcal surface protein A (PspA) has been shown to provide protective immunity against pneumococcal infection and is considered as a pneumococcal vaccine. The protein release experiments demonstrated that from an initial pH 7.4, approximately 60% of PspA diffuse into a neutral environment with an initial burst and a declining rate reaching equilibrium. The results indicate that the protein was successfully incorporated and released from the membrane over time. The hydrogel and protein interaction is temporary, and the membrane system is ideal for protein drug delivery. The data confirm that the protein did not aggregate and was active after release. The protein release is promising and a step forward to develop microneedles to facilitate high molecular weight protein delivery as well as vaccine delivery.
{"title":"Study of Pneumococcal Surface Protein, PspA, Incorporated in Poly(Vinyl Alcohol) Hydrogel Membranes","authors":"Hazim S. Aljewari, MT Romo Castro, Olivia Solomon, Quincy C. Moore, F. Nave, A. Thompson","doi":"10.4236/jbnb.2020.111005","DOIUrl":"https://doi.org/10.4236/jbnb.2020.111005","url":null,"abstract":"This study investigates poly(vinyl alcohol) (PVA) membranes as controlled release micro-matrices, which can be useful in therapeutic applications for optimizing the administration of drugs. Currently, the use of hydrogels is limited by protein size. This study investigates the delivery of PspA, a large protein of approximately 38 kD. Pneumococcal surface protein A (PspA) has been shown to provide protective immunity against pneumococcal infection and is considered as a pneumococcal vaccine. The protein release experiments demonstrated that from an initial pH 7.4, approximately 60% of PspA diffuse into a neutral environment with an initial burst and a declining rate reaching equilibrium. The results indicate that the protein was successfully incorporated and released from the membrane over time. The hydrogel and protein interaction is temporary, and the membrane system is ideal for protein drug delivery. The data confirm that the protein did not aggregate and was active after release. The protein release is promising and a step forward to develop microneedles to facilitate high molecular weight protein delivery as well as vaccine delivery.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44456133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.4236/jbnb.2020.111004
A. A. Ntoumba, F. E. Meva, W. Ekoko, L. Foko, Etoile Odette Ngo Hondt, Carsten Schlüsener, Bastian Moll, G. Loé, Philippe Belle Ebanda Kedi, Jean Yves Sikapi Fouda, C. Janiak, L. Lehman
The progress in the field of nanotechnology has contributed to the development of tools for combating the most critical problems in developing countries. The requirements that such tools should meet are low-cost and resource settings, environmental protection, ease of use, and availability. The use of plant properties for the generation of nanoparticles (NPs), which serve as bioinsecticides to combat the plasticity and resistance of mosquitoes and parasites, is considered possible. Here, we report for the first time the larvicidal activity of silver (Ag) NPs (AgNPs) synthesized from Psidium guajava (P. guajava) extract, which targets the 4th instar larvae of Anopheles gambiae. Concentrations of AgNPs between 0 and 200 ppm were used and their LC50 at 24 h and 48 h were determined as 19.55 ppm and 8.737 ppm, respectively. The AgNPs were stable and highly effective against the larvae of A. gambiae and thereby we anticipate that they can be used to combat vector-borne diseases in developing countries.
{"title":"Biogenic Synthesis of Silver Nanoparticles Using Guava (Psidium guajava) Leaf Extract and Its Larvicidal Action against Anopheles gambiae","authors":"A. A. Ntoumba, F. E. Meva, W. Ekoko, L. Foko, Etoile Odette Ngo Hondt, Carsten Schlüsener, Bastian Moll, G. Loé, Philippe Belle Ebanda Kedi, Jean Yves Sikapi Fouda, C. Janiak, L. Lehman","doi":"10.4236/jbnb.2020.111004","DOIUrl":"https://doi.org/10.4236/jbnb.2020.111004","url":null,"abstract":"The progress in the field of nanotechnology has contributed to the development of tools for combating the most critical problems in developing countries. The requirements that such tools should meet are low-cost and resource settings, environmental protection, ease of use, and availability. The use of plant properties for the generation of nanoparticles (NPs), which serve as bioinsecticides to combat the plasticity and resistance of mosquitoes and parasites, is considered possible. Here, we report for the first time the larvicidal activity of silver (Ag) NPs (AgNPs) synthesized from Psidium guajava (P. guajava) extract, which targets the 4th instar larvae of Anopheles gambiae. Concentrations of AgNPs between 0 and 200 ppm were used and their LC50 at 24 h and 48 h were determined as 19.55 ppm and 8.737 ppm, respectively. The AgNPs were stable and highly effective against the larvae of A. gambiae and thereby we anticipate that they can be used to combat vector-borne diseases in developing countries.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.4236/jbnb.2020.114016
E. R. Vickers
Biodegradable scaffolds have a major therapeutic advantage in regenerative medicine with their ability to include multiple compounds of drugs, growth factors and more recently, stem cells within the matrix. The scaffold can be programmed with mechanoresistive parameters targeted to the tissue to be replaced. Direct chemoattraction of in vivo stem cells to the implanted scaffold would be advantageous in the clinical setting. Large peptides such as vasculo-endothelial growth factor have demonstrated chemotaxis for angiogenesis from endothelial cells. This suggests other endogenous peptides may be present to directly attract stem cells to a scaffold. This exploratory study assessed if peptides from the blood peptidome would display chemotaxis to stem cells. Results showed that several short N-mer peptides demonstrated remarkable chemotaxis to blood and adipose tissue derived stem cells. Sodium alginate hydrogel was placed into 6-well, 24-well plate, and partitioned plates with channels between the wells. Connected wells were in series and spiked with peptides, biofluids containing stem cells and control wells. Images were recorded between three and nine days after incubation at 37°C. There were rapid migration and expansion of stem cells into the peptide wells. Cell analysis revealed activated stem cells on a number of parameters including autophagy, Ki67 and nitric oxide. Potentially, this enhanced method to bioscaffold design utilizing peptide chemoattraction could result in an improved approach for stem cell therapy and regenerative medicine applications. Specific patient groups (e.g. blood coagulation disorders) where surgery to acquire adipose tissue or bone marrow is contraindicated may benefit. In addition, the technology is portable and safe by using “on demand” peripheral blood derived stem cells and would be particularly suitable for specialized environments such as space medicine.
{"title":"Peptidic Alginate-Based Hydrogels Demonstrate Chemotaxis and Expansion to Adipose Tissue Derived and Blood Derived Stem Cells","authors":"E. R. Vickers","doi":"10.4236/jbnb.2020.114016","DOIUrl":"https://doi.org/10.4236/jbnb.2020.114016","url":null,"abstract":"Biodegradable scaffolds have a major therapeutic advantage in regenerative medicine with their ability to include multiple compounds of drugs, growth factors and more recently, stem cells within the matrix. The scaffold can be programmed with mechanoresistive parameters targeted to the tissue to be replaced. Direct chemoattraction of in vivo stem cells to the implanted scaffold would be advantageous in the clinical setting. Large peptides such as vasculo-endothelial growth factor have demonstrated chemotaxis for angiogenesis from endothelial cells. This suggests other endogenous peptides may be present to directly attract stem cells to a scaffold. This exploratory study assessed if peptides from the blood peptidome would display chemotaxis to stem cells. Results showed that several short N-mer peptides demonstrated remarkable chemotaxis to blood and adipose tissue derived stem cells. Sodium alginate hydrogel was placed into 6-well, 24-well plate, and partitioned plates with channels between the wells. Connected wells were in series and spiked with peptides, biofluids containing stem cells and control wells. Images were recorded between three and nine days after incubation at 37°C. There were rapid migration and expansion of stem cells into the peptide wells. Cell analysis revealed activated stem cells on a number of parameters including autophagy, Ki67 and nitric oxide. Potentially, this enhanced method to bioscaffold design utilizing peptide chemoattraction could result in an improved approach for stem cell therapy and regenerative medicine applications. Specific patient groups (e.g. blood coagulation disorders) where surgery to acquire adipose tissue or bone marrow is contraindicated may benefit. In addition, the technology is portable and safe by using “on demand” peripheral blood derived stem cells and would be particularly suitable for specialized environments such as space medicine.","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"11 1","pages":"245-259"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896748","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.4236/jbnb.2020.114014
Harleen Kaur, Pranav Pancham, R. Kaur, S. Agarwal, Manisha Singh
{"title":"Synthesis and Characterization of Citrus limonum Essential Oil Based Nanoemulsion and Its Enhanced Antioxidant Activity with Stability for Transdermal Application","authors":"Harleen Kaur, Pranav Pancham, R. Kaur, S. Agarwal, Manisha Singh","doi":"10.4236/jbnb.2020.114014","DOIUrl":"https://doi.org/10.4236/jbnb.2020.114014","url":null,"abstract":"","PeriodicalId":68623,"journal":{"name":"生物材料与纳米技术(英文)","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"70896665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2020-01-01DOI: 10.4236/jbnb.2020.111002
R. Cabello, J. Vega-baudrit, R. Zuluaga, P. Gañán
The need to have an express regulation covering nanotechnology has been the subject of debate in the scientific literature and identified as one of the main subsets of nanotechnology field research. However, most countries still do not have regulatory framework in order to guarantee consumer safety. This is the case of Costa Rica, one of the most promising countries in Latin America in terms of biotechnology and nanotechnology. This article presents a statistical study about the position of industry, academia and government institutions on the need to expressly regulate nanotechnology in Costa Rica. A qualitative study consisting of a survey of 79 forms was done to individuals representing the community involved with nanotechnology and institutions responsible for ensuring the safety of the citizen’s health, to conclude that the nanotechnology regulation should be created to protect the consumer in Costa Rica. The research also proposes aspects that should be taken into account in its drafting as well as the variables on which decisions should be made to authorize the commercialization of nanomaterials based on the findings of the literature.
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