Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.01034
Yong Liao, Qing-long Tang, Jian-jun Li, Yi Luo, Rongya Yang
{"title":"A PubMed-based bibliometric analysis on research of pruritus","authors":"Yong Liao, Qing-long Tang, Jian-jun Li, Yi Luo, Rongya Yang","doi":"10.3724/SP.J.1008.2015.01034","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01034","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"1034"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69858303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.01102
Chuan-chuan Wang, Mi-rui Qu, Qinghai Huang
Identifying patients with rupture-prone intracranial aneurysms and giving pertinent appropriate therapies are the keys to prevent aneurysm rupture and reduce the morbidity and mortality.Progression in magnetic resonance imaging has resulted in increasingly application of it for intracranial aneurysms.This review discussed the recent progress on the role of high-resolution MRI in evaluating intracranial aneurysm rupture risk.
{"title":"High-resolution MRI for evaluation of intracranial aneurysm rupture risk:recent progress","authors":"Chuan-chuan Wang, Mi-rui Qu, Qinghai Huang","doi":"10.3724/SP.J.1008.2015.01102","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01102","url":null,"abstract":"Identifying patients with rupture-prone intracranial aneurysms and giving pertinent appropriate therapies are the keys to prevent aneurysm rupture and reduce the morbidity and mortality.Progression in magnetic resonance imaging has resulted in increasingly application of it for intracranial aneurysms.This review discussed the recent progress on the role of high-resolution MRI in evaluating intracranial aneurysm rupture risk.","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"1102"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69858861","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.00136
B. Tan, L. Yong, L. Fan, Qun Zhao, Dong Wang, Yu Liu
Objective To explore the role of Vav3 gene in multidrug resistance(MDR)of gastric cancer and the related mechanism.Methods QRT-PCR was used to examine the expressions of Vav3 gene in gastric cancer tissues,tumor-adjacent tissues,human gastric cancer cell line SGC7901,and gastric epithelial cell line GES-1.Then Vav3-siRNA was synthesized and tansfected into SGC7901 cells. MTT assay was then used to determine the inhibition rates of tumor cells exposed to chemotherapeutic agents(5-FU,L-OHP)before and after Vav3-siRNA transfection.Real-time RT-PCR and Western blotting analysis were used to observe the expressions of inhibitor of apoptosis proteins(IAPs):xIAP,Survivin,and Livin;meanwhile,the expression and activity of Caspase-3and Caspase-8 were also determined.Results Vav3 was over-expressed in gastric cancer tissues and gastric cell line compared with those in tumor-adjacent tissues and gastric epithelial cell line GES-1(P0.05).Expression of Vav3 was significantly inhibited by Vav3-siRNA(P0.01).Inhibition rates of tumor cells exposed to 5-FU and L-OHP were significantly increased 48 hafter Vav3-siRNA tansfection(P0.05).The expressions of xIAP and Survivin were significantly decreased in cancer cells after Vav3-siRNA tansfection(both P0.05),and no notable change was found for Livin expression;also the expression and activity of Caspase-3and Caspase-8protein were significantly increased after Vav3-siRNA tansfection in SGC7901cells(all P0.05).Conclusion Vav3 can participate in MDR of gastric cancer by regulating apoptotic pathways,and inhibition of Vav3 can help reverse MDR of gastric cancer cells by regulating some IAPs.
{"title":"Effect of Vav3 gene inhibition on inhibitor of apoptosis proteins and multidrug resistance in gastric cancer cells","authors":"B. Tan, L. Yong, L. Fan, Qun Zhao, Dong Wang, Yu Liu","doi":"10.3724/SP.J.1008.2015.00136","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00136","url":null,"abstract":"Objective To explore the role of Vav3 gene in multidrug resistance(MDR)of gastric cancer and the related mechanism.Methods QRT-PCR was used to examine the expressions of Vav3 gene in gastric cancer tissues,tumor-adjacent tissues,human gastric cancer cell line SGC7901,and gastric epithelial cell line GES-1.Then Vav3-siRNA was synthesized and tansfected into SGC7901 cells. MTT assay was then used to determine the inhibition rates of tumor cells exposed to chemotherapeutic agents(5-FU,L-OHP)before and after Vav3-siRNA transfection.Real-time RT-PCR and Western blotting analysis were used to observe the expressions of inhibitor of apoptosis proteins(IAPs):xIAP,Survivin,and Livin;meanwhile,the expression and activity of Caspase-3and Caspase-8 were also determined.Results Vav3 was over-expressed in gastric cancer tissues and gastric cell line compared with those in tumor-adjacent tissues and gastric epithelial cell line GES-1(P0.05).Expression of Vav3 was significantly inhibited by Vav3-siRNA(P0.01).Inhibition rates of tumor cells exposed to 5-FU and L-OHP were significantly increased 48 hafter Vav3-siRNA tansfection(P0.05).The expressions of xIAP and Survivin were significantly decreased in cancer cells after Vav3-siRNA tansfection(both P0.05),and no notable change was found for Livin expression;also the expression and activity of Caspase-3and Caspase-8protein were significantly increased after Vav3-siRNA tansfection in SGC7901cells(all P0.05).Conclusion Vav3 can participate in MDR of gastric cancer by regulating apoptotic pathways,and inhibition of Vav3 can help reverse MDR of gastric cancer cells by regulating some IAPs.","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"136"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69844428","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.00115
S. Deng, Jing-hui Li, Tao Sun, Hua-lin Yu, Yi-liu Ma, Ying Yang
{"title":"Endoscopic resection of third ventricular colloid cysts: a case report","authors":"S. Deng, Jing-hui Li, Tao Sun, Hua-lin Yu, Yi-liu Ma, Ying Yang","doi":"10.3724/SP.J.1008.2015.00115","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00115","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"115"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69844457","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.00346
Jing Yang, Mian-hua Wu, Qi Zhang, Xiangcheng Wang
{"title":"First line XELOX chemotherapy combined with subsequent capecitabine maintenance chemotherapy for patients with advanced gastric cancer: a clinical analysis of effectiveness","authors":"Jing Yang, Mian-hua Wu, Qi Zhang, Xiangcheng Wang","doi":"10.3724/SP.J.1008.2015.00346","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00346","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"346"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69845938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.00581
Cheng-zhong Li
{"title":"Thinking after fighting against 2014 Ebola epidemic in Liberia","authors":"Cheng-zhong Li","doi":"10.3724/SP.J.1008.2015.00581","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.00581","url":null,"abstract":"","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"581"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69849804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Objective To study the effect of nano-silver active carbon fiber dressings on wound healing in bedsore wound animal models.Methods Adult SD rats were used and bedsore wound models were made on their back;the animals were then randomly divided into 5groups(n=10)according to different materials of the wound dressing and treatment.Groups were treated with nano-silver active carbon dressings,nano-silver dressings,active carbon dressings,Vaseline chlorhexidine dressings,or blank control.The wound healing situation,contents of angiogenic factors and inflammatory factors in wound tissues were compared among the 5groups.Results Wound healing time and the healing rates were different among the 5groups,with the healing time of rats in nano-silver active carbon dressing group being significantly shorter than those in the other 4groups(P0.05).On the 3rd,7th,14 th and 21 st days after bedsore wound modeling,the healing rates of nano-silver active carbon dressing group were significantly higher than those of the other 4groups(P0.05).The levels of angiogenesis factors and inflammatory factors were different in wound tissues of five groups,with the contents of VEGFA,VEGFB and VEGFC mRNA in nano-silver active carbon dressing group being significantly higher and the contents of TNF-α,IL-2and IL-8mRNA being significantly lower than those of the other 4groups(P0.05).Conclusion The prepared nano-silver active carbon fiber dressing can help to improve pressure ulcer wound healing,relieve wound inflammation,and promote angiogenesis in the wound;it may serve as an ideal material for treating bedsore wound.
{"title":"Nano-silver active carbon fiber dressings improving bedsore wound healing in rats","authors":"Ying Wang, Jian-xing Song, Jiang-ping Chen, Xiaojin Lou","doi":"10.3724/SP.J.1008.2015.01051","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01051","url":null,"abstract":"Objective To study the effect of nano-silver active carbon fiber dressings on wound healing in bedsore wound animal models.Methods Adult SD rats were used and bedsore wound models were made on their back;the animals were then randomly divided into 5groups(n=10)according to different materials of the wound dressing and treatment.Groups were treated with nano-silver active carbon dressings,nano-silver dressings,active carbon dressings,Vaseline chlorhexidine dressings,or blank control.The wound healing situation,contents of angiogenic factors and inflammatory factors in wound tissues were compared among the 5groups.Results Wound healing time and the healing rates were different among the 5groups,with the healing time of rats in nano-silver active carbon dressing group being significantly shorter than those in the other 4groups(P0.05).On the 3rd,7th,14 th and 21 st days after bedsore wound modeling,the healing rates of nano-silver active carbon dressing group were significantly higher than those of the other 4groups(P0.05).The levels of angiogenesis factors and inflammatory factors were different in wound tissues of five groups,with the contents of VEGFA,VEGFB and VEGFC mRNA in nano-silver active carbon dressing group being significantly higher and the contents of TNF-α,IL-2and IL-8mRNA being significantly lower than those of the other 4groups(P0.05).Conclusion The prepared nano-silver active carbon fiber dressing can help to improve pressure ulcer wound healing,relieve wound inflammation,and promote angiogenesis in the wound;it may serve as an ideal material for treating bedsore wound.","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"1051"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69857982","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-01-01DOI: 10.3724/SP.J.1008.2015.01092
L. Xian
Objective To lay a foundation for the industrial fermentation and vaccine development of Pseudomonas aeruginosa(P.oeruginosa)outer membrane protein OprH.Methods The OprH expression strain was obtained by molecular cloning.The culture condition and optimal expression of the experiment was obtained by the method of orthogonal design.OprH was purified by gel slice strategy and was used to immunize mice to prepare the polyclonal antibody.The antibody titer and specificity were detected by ELISA and Western blotting analysis,respectively.Mice were immunized by OprH protein and infected with P.aeruginosa,and the immune protection of OprH was detected.Results OprH recombinant vector were digested and sequenced,and the results confirmed the correct construction;and OprH expression and purification of strip size agreed with the prediction.The optimal culture condition was as follows:rotation rate was 230r/min,glucose concentration was 0%,and the medium volume was 50 mL.The optimal inducing expression condition of OprH was as follows:isopropy-β-Dthiogalactoside final concentration was 0.3mmol/L,strain D600 value was 0.8,inducing temperature was 32℃,and inducing time was 3h.The OprH antibody titer was 1∶1 600 as detected by ELISA,and Western blotting analysis proved that the antiserum had good specificity.Mice specific immune was activated by OprH,and immune protection rate for mice against P.aeruginosainfection was 46.15 %,which had significant differences compared with the control(P0.05).Conclusion We have successfully cloned OprH expression vector,purified OprH,prepared the polyclonal antibodies of OprH.It is confirmed that OprH protein has significant immune protection against P.aeruginosa,and the culture and induction conditions of the recombinant OprH have been obtained.
{"title":"Prokaryotic expression,polyclonal antibody preparation and immunoprotection potential of Pseudomonas aeruginosa outer membrane protein OprH","authors":"L. Xian","doi":"10.3724/SP.J.1008.2015.01092","DOIUrl":"https://doi.org/10.3724/SP.J.1008.2015.01092","url":null,"abstract":"Objective To lay a foundation for the industrial fermentation and vaccine development of Pseudomonas aeruginosa(P.oeruginosa)outer membrane protein OprH.Methods The OprH expression strain was obtained by molecular cloning.The culture condition and optimal expression of the experiment was obtained by the method of orthogonal design.OprH was purified by gel slice strategy and was used to immunize mice to prepare the polyclonal antibody.The antibody titer and specificity were detected by ELISA and Western blotting analysis,respectively.Mice were immunized by OprH protein and infected with P.aeruginosa,and the immune protection of OprH was detected.Results OprH recombinant vector were digested and sequenced,and the results confirmed the correct construction;and OprH expression and purification of strip size agreed with the prediction.The optimal culture condition was as follows:rotation rate was 230r/min,glucose concentration was 0%,and the medium volume was 50 mL.The optimal inducing expression condition of OprH was as follows:isopropy-β-Dthiogalactoside final concentration was 0.3mmol/L,strain D600 value was 0.8,inducing temperature was 32℃,and inducing time was 3h.The OprH antibody titer was 1∶1 600 as detected by ELISA,and Western blotting analysis proved that the antiserum had good specificity.Mice specific immune was activated by OprH,and immune protection rate for mice against P.aeruginosainfection was 46.15 %,which had significant differences compared with the control(P0.05).Conclusion We have successfully cloned OprH expression vector,purified OprH,prepared the polyclonal antibodies of OprH.It is confirmed that OprH protein has significant immune protection against P.aeruginosa,and the culture and induction conditions of the recombinant OprH have been obtained.","PeriodicalId":6893,"journal":{"name":"海军军医大学学报","volume":"36 1","pages":"1092"},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69858667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：