Pub Date : 2023-05-31DOI: 10.37427/botcro-2023-012
Nika Pasković, Iris Dupčić Radić
This report presents the first record of Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea. The species was found in the net sample in the layer of 50 – 100 m depth, on July 2021 at the coastal southern Adriatic Sea. The species T. rotundatus was probably previously mistaken as morphologically similar species T. digitatus (Schütt) Gómez.
{"title":"First record of the dinoflagellate Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea","authors":"Nika Pasković, Iris Dupčić Radić","doi":"10.37427/botcro-2023-012","DOIUrl":"https://doi.org/10.37427/botcro-2023-012","url":null,"abstract":"This report presents the first record of Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea. The species was found in the net sample in the layer of 50 – 100 m depth, on July 2021 at the coastal southern Adriatic Sea. The species T. rotundatus was probably previously mistaken as morphologically similar species T. digitatus (Schütt) Gómez.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48646346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.37427/botcro-2023-011
Yu. O. Bilonozhko, T. Krupodorova, A. Rabokon, A. Postovoitova, L. Kalafat, Y. Pirko, Y. Blume
To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.
{"title":"In vitro cultivation and biocontrol potential of Botryosphaeria visci against European mistletoe (Viscum album L.)","authors":"Yu. O. Bilonozhko, T. Krupodorova, A. Rabokon, A. Postovoitova, L. Kalafat, Y. Pirko, Y. Blume","doi":"10.37427/botcro-2023-011","DOIUrl":"https://doi.org/10.37427/botcro-2023-011","url":null,"abstract":"To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41299989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.37427/botcro-2023-008
D. Pavoković, A. Horvatić, I. Tomljanović, B. Balen, M. Krsnik-Rasol
Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.
{"title":"Sugar beet cells cellular and extracellular events taking place in response to drought and salinity","authors":"D. Pavoković, A. Horvatić, I. Tomljanović, B. Balen, M. Krsnik-Rasol","doi":"10.37427/botcro-2023-008","DOIUrl":"https://doi.org/10.37427/botcro-2023-008","url":null,"abstract":"Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41290715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.37427/botcro-2023-009
YU-JIE Li, JI-SHU Guo, HONG-PING Ni, YING-YAN Huang, J. Kociolek, YAN-LING Li
One new species, Placoneis modaomensis, found in freshwater environment from tributary of the Pearl River which lies in Modaomen Channel, Zhuhai County, Guangdong Province, China, is described based on light and scanning electron microscope observations. P. modaomensis sp. nov. has the morphological features that are typical for the genus, including external terminal raphe fissures curve to the opposite sides and areolae with internal volate occlusions. The new species is similar to P. amphibola (Cleve) E.J. Cox, P. amphiboliformis (Metzeltin, Lange-Bertalot & Soninkhishig) Vishnyakov, P. parvapolonica Lange-Bertalot & Wojtal, P. clementispronina Lange-Bertalot & Wojtal and P. nanoclementis Lange-Bertalot & Wojtal in the shape of the valves and having course striae but it can be easily distinguished by the two main morphological characteristics: external central raphe endings bent in the opposite directions, and areolae covered by volate occlusions externally. The latter feature appears to be new for taxa assigned to the genus Placoneis. Data on the associated diatom flora and its ecology are also given. These findings increase our understanding about the morphology of Placoneis in general and its and distribution of the genus in China.
根据光镜和扫描电镜观察，介绍了在广东省珠海县磨门海峡珠江支流淡水环境中发现的一个新种——磨门扁螺。P.modaomensis sp.nov.具有该属典型的形态特征，包括向外弯曲的中缝末端裂隙和具有内掌闭塞的乳晕。该新种类似于角闪石P.（Cleve）E.J.Cox、角闪石P，P.clementistronina Lange Bertalot&Wojtal和P.nanoclementis Lange Berdalot&Woj tal呈瓣膜形状，具有行程纹，但可以很容易地通过两个主要的形态学特征来区分：外侧中央中缝末端向相反方向弯曲，乳晕外侧被掌状闭塞物覆盖。后一个特征似乎是新的分类群，被分配到Placoneis属。还提供了有关硅藻植物群及其生态学的数据。这些发现增加了我们对斑蝶属的一般形态及其在中国的分布的了解。
{"title":"Placoneis modaomensis sp. nov. (Bacillariophyta; Cymbellaceae), a new species from Guangdong Province, China","authors":"YU-JIE Li, JI-SHU Guo, HONG-PING Ni, YING-YAN Huang, J. Kociolek, YAN-LING Li","doi":"10.37427/botcro-2023-009","DOIUrl":"https://doi.org/10.37427/botcro-2023-009","url":null,"abstract":"One new species, Placoneis modaomensis, found in freshwater environment from tributary of the Pearl River which lies in Modaomen Channel, Zhuhai County, Guangdong Province, China, is described based on light and scanning electron microscope observations. P. modaomensis sp. nov. has the morphological features that are typical for the genus, including external terminal raphe fissures curve to the opposite sides and areolae with internal volate occlusions. The new species is similar to P. amphibola (Cleve) E.J. Cox, P. amphiboliformis (Metzeltin, Lange-Bertalot & Soninkhishig) Vishnyakov, P. parvapolonica Lange-Bertalot & Wojtal, P. clementispronina Lange-Bertalot & Wojtal and P. nanoclementis Lange-Bertalot & Wojtal in the shape of the valves and having course striae but it can be easily distinguished by the two main morphological characteristics: external central raphe endings bent in the opposite directions, and areolae covered by volate occlusions externally. The latter feature appears to be new for taxa assigned to the genus Placoneis. Data on the associated diatom flora and its ecology are also given. These findings increase our understanding about the morphology of Placoneis in general and its and distribution of the genus in China.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45474679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-15DOI: 10.37427/botcro-2023-010
E. C. Aytar, Yasemin ÖZDENER KÖMPE
In recent years, the orchid species have become endangered due to overuse and habitat destruction. As with most flowering plants, seed production is the primary strategy for reproduction in orchids. Orchids produce tiny seeds consisting of a seed coat and a rudimentary embryo. However, it lacks the endosperm, which is generally required as the primary energy source during germination. The only way to germinate orchid seeds is to get nutrients from an external source. In nature, this is achieved by mycorrhizal symbiosis. This study used Ceratobasidium sp. inoculation of Anacamptis laxiflora (Lam.) seeds combined with media with various organic substrates to determine their effectiveness on germination and seedling development by in vitro culture. The highest germination rate (35.78%) was obtained in the medium with addition of young hazelnut leaves. Then, soilless ex vitro symbiotic germination was performed on young hazelnut leaves, the most effective organic substrate. Seed germination was determined to be 19.01% in this medium while 14.87% seedlings with developed leaves and roots were formed. For the first time, success was achieved by producing A. laxiflora from seed in ex vitro conditions without soil and adapting it to nature.
{"title":"Effect of different substrates on in vitro symbiotic seed germination for soilless production of Anacamptis laxiflora orchid","authors":"E. C. Aytar, Yasemin ÖZDENER KÖMPE","doi":"10.37427/botcro-2023-010","DOIUrl":"https://doi.org/10.37427/botcro-2023-010","url":null,"abstract":"In recent years, the orchid species have become endangered due to overuse and habitat destruction. As with most flowering plants, seed production is the primary strategy for reproduction in orchids. Orchids produce tiny seeds consisting of a seed coat and a rudimentary embryo. However, it lacks the endosperm, which is generally required as the primary energy source during germination. The only way to germinate orchid seeds is to get nutrients from an external source. In nature, this is achieved by mycorrhizal symbiosis. This study used Ceratobasidium sp. inoculation of Anacamptis laxiflora (Lam.) seeds combined with media with various organic substrates to determine their effectiveness on germination and seedling development by in vitro culture. The highest germination rate (35.78%) was obtained in the medium with addition of young hazelnut leaves. Then, soilless ex vitro symbiotic germination was performed on young hazelnut leaves, the most effective organic substrate. Seed germination was determined to be 19.01% in this medium while 14.87% seedlings with developed leaves and roots were formed. For the first time, success was achieved by producing A. laxiflora from seed in ex vitro conditions without soil and adapting it to nature.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47455474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-29DOI: 10.37427/botcro-2023-007
N. Jasprica, M. Terzi
This paper presents the new association Pimpinello lithophilae-Centaureetum lovricii, described for the halotolerant vegetation of the order Helichrysetalia italici (Crithmo-Staticetea) on the island of Vis (southern Croatia). The new association substitutes the Pimpinello lithophilae-Centaureetum issaeae, originally published invalidly because its name was formed from the invalid taxon name ‘Centaurea issaea Lovrić’.
{"title":"The new association Pimpinello lithophilae-Centaureetum lovricii (Crithmo-Staticetea) from the island of Vis (southern Croatia)","authors":"N. Jasprica, M. Terzi","doi":"10.37427/botcro-2023-007","DOIUrl":"https://doi.org/10.37427/botcro-2023-007","url":null,"abstract":"This paper presents the new association Pimpinello lithophilae-Centaureetum lovricii, described for the halotolerant vegetation of the order Helichrysetalia italici (Crithmo-Staticetea) on the island of Vis (southern Croatia). The new association substitutes the Pimpinello lithophilae-Centaureetum issaeae, originally published invalidly because its name was formed from the invalid taxon name ‘Centaurea issaea Lovrić’.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45985036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-08DOI: 10.37427/botcro-2023-006
F. Nazari, R. Hajiboland, C. Poschenrieder, E. Kahneh, S. Salehi-Lisar, Aioub Moradi
About one-third of the earth’s land area consists of acidic soils. The rhizotoxic Al3+ is one of the primary constraints associated with low soil pH. Various Amaranthus species are important components of the weed flora in tea plantations on acid soils in north Iran. In this study, four Amaranthus species (A. blitoides, A. retroflexus, A. cruentus, and A. tricolor) were grown under hydroponic conditions with total Al concentrations of 0, 20, 50, 200, and 400 µM corresponding to free Al3+ activity of 0, 3.75, 11.97, 60.34, and 125 µM, respectively. Low Al concentrations (20, 50, or 200 µM) stimulated plant growth, A. tricolor demonstrated the highest improvement in shoot growth (93%), whereas A. retroflexus exhibited the greatest improvement in root biomass (367%), total root length (173%), and aproot length (32%). Although the response of shoot biomass to 400 µM Al varied among species, all species were able to accumulate Al in the leaves above the critical level considered for Al hyperaccumulation (1 mg g–1 DW). Our findings revealed Al accumulation in Amaranthus species for the first time at the genus and family levels, suggesting that these species are suitable for the restoration and revegetation of acid-eroded soils.
地球陆地面积的三分之一是酸性土壤。根毒性Al3+是低土壤ph的主要制约因素之一。伊朗北部酸性土壤茶园中，各种苋属植物是杂草区系的重要组成部分。以四种苋菜(A. blitoides, A. retroflexus, A. cruentus和A. tricolor)为研究对象，在总铝浓度为0、20、50、200和400µM的水培条件下生长，其游离Al3+活性分别为0、3.75、11.97、60.34和125µM。低铝浓度(20、50或200µM)对植物生长有促进作用，三色木犀对茎部生长的促进作用最大(93%)，而逆行木犀对根生物量(367%)、总根长(173%)和主根长(32%)的促进作用最大。尽管不同物种的茎部生物量对400µM Al的响应不同，但所有物种在叶片中积累的Al都超过了过量积累的临界水平(1 mg g-1 DW)。研究结果首次揭示了苋属植物在属和科水平上的铝积累，表明苋属植物适合于酸侵蚀土壤的恢复和恢复。
{"title":"Aluminum accumulation and tolerance in four Amaranthus species","authors":"F. Nazari, R. Hajiboland, C. Poschenrieder, E. Kahneh, S. Salehi-Lisar, Aioub Moradi","doi":"10.37427/botcro-2023-006","DOIUrl":"https://doi.org/10.37427/botcro-2023-006","url":null,"abstract":"About one-third of the earth’s land area consists of acidic soils. The rhizotoxic Al3+ is one of the primary constraints associated with low soil pH. Various Amaranthus species are important components of the weed flora in tea plantations on acid soils in north Iran. In this study, four Amaranthus species (A. blitoides, A. retroflexus, A. cruentus, and A. tricolor) were grown under hydroponic conditions with total Al concentrations of 0, 20, 50, 200, and 400 µM corresponding to free Al3+ activity of 0, 3.75, 11.97, 60.34, and 125 µM, respectively. Low Al concentrations (20, 50, or 200 µM) stimulated plant growth, A. tricolor demonstrated the highest improvement in shoot growth (93%), whereas A. retroflexus exhibited the greatest improvement in root biomass (367%), total root length (173%), and aproot length (32%). Although the response of shoot biomass to 400 µM Al varied among species, all species were able to accumulate Al in the leaves above the critical level considered for Al hyperaccumulation (1 mg g–1 DW). Our findings revealed Al accumulation in Amaranthus species for the first time at the genus and family levels, suggesting that these species are suitable for the restoration and revegetation of acid-eroded soils.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48488883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-08DOI: 10.37427/botcro-2023-005
Gregorio Aragón, Isabel Martínez
The present paper reports the first record of Diplotomma cedricola (Werner) Etayo in the eastern Mediterranean (Creta Island, Greece), being the nearest record from Corsica (France). This lichen species was found near Iérapreta on decorticated Cupressus sempervirens L. and Pinus brutia Ten. trees. This finding constitutes a great expansion of the distribution range of this species.
{"title":"First record of Diplotomma cedricola in the eastern Mediterranean","authors":"Gregorio Aragón, Isabel Martínez","doi":"10.37427/botcro-2023-005","DOIUrl":"https://doi.org/10.37427/botcro-2023-005","url":null,"abstract":"The present paper reports the first record of Diplotomma cedricola (Werner) Etayo in the eastern Mediterranean (Creta Island, Greece), being the nearest record from Corsica (France). This lichen species was found near Iérapreta on decorticated Cupressus sempervirens L. and Pinus brutia Ten. trees. This finding constitutes a great expansion of the distribution range of this species.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44410511","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Benzoapyrene (B[a]P) pollution poses a threat to the environment and the food chain and consequently to human health. However, the alleviation of the harmful effects of B[a]P pollution in perennial ryegrass (Lolium perenne L.) by the application of exogenous nitric oxide (NO) has been ignored. Thus, in this paper the effects of exogenous sodium nitroprusside (SNP, a NO donor) on the growth, photosynthetic fluorescence characteristics, and antioxidant enzyme activity of ryegrass exposed to B[a]P stress are investigated. B[a]P stress induced the reduction of the aboveground and belowground dry weights, chlorophyll (a, b), the total chlorophyll contents, the carotenoid content, the net photosynthetic rate (Pn), the intercellular carbon dioxide concentration (Ci), the water use efficiency (WUE), the photosystem II (PSII) potential activity (Fv/F0), the maximum quantum yield of PSII photochemistry (Fv/Fm), the steady-state fluorescence yield (Fs), and the non-photochemical quenching (qN), while enhancement was recorded in response to the foliar spray of SNP at 200 and 300 μmol·L-1 under B[a]P stress. Gray correlation and principal component analyses show that 200 μmol·L 1 of SNP more drastically alleviated the damage caused by B[a]P stress than 300 μmol·L 1 of SNP. The exogenous NO-mediated alleviation of B[a]P toxicity in ryegrass was associated with preserved photosynthetic characteristics and activation of antioxidant enzymes.
{"title":"Effects of exogenous NO on the growth and photosynthetic fluorescence characteristics of ryegrass seedlings under B[a]P stress","authors":"Yue Li, Junqiang Ma, Yu Wang, Sunan Xu, Lei Jiang, Lihong Zhang, Wei Hou","doi":"10.37427/botcro-2023-004","DOIUrl":"https://doi.org/10.37427/botcro-2023-004","url":null,"abstract":"Benzoapyrene (B[a]P) pollution poses a threat to the environment and the food chain and consequently to human health. However, the alleviation of the harmful effects of B[a]P pollution in perennial ryegrass (Lolium perenne L.) by the application of exogenous nitric oxide (NO) has been ignored. Thus, in this paper the effects of exogenous sodium nitroprusside (SNP, a NO donor) on the growth, photosynthetic fluorescence characteristics, and antioxidant enzyme activity of ryegrass exposed to B[a]P stress are investigated. B[a]P stress induced the reduction of the aboveground and belowground dry weights, chlorophyll (a, b), the total chlorophyll contents, the carotenoid content, the net photosynthetic rate (Pn), the intercellular carbon dioxide concentration (Ci), the water use efficiency (WUE), the photosystem II (PSII) potential activity (Fv/F0), the maximum quantum yield of PSII photochemistry (Fv/Fm), the steady-state fluorescence yield (Fs), and the non-photochemical quenching (qN), while enhancement was recorded in response to the foliar spray of SNP at 200 and 300 μmol·L-1 under B[a]P stress. Gray correlation and principal component analyses show that 200 μmol·L 1 of SNP more drastically alleviated the damage caused by B[a]P stress than 300 μmol·L 1 of SNP. The exogenous NO-mediated alleviation of B[a]P toxicity in ryegrass was associated with preserved photosynthetic characteristics and activation of antioxidant enzymes.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":null,"pages":null},"PeriodicalIF":1.3,"publicationDate":"2023-02-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47731936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2022-12-22DOI: 10.37427/botcro-2023-003
M. Mačukanović-Jocić, D. Stešević, D. Rančić, M. Šundiċ
The pollen grains of Leiotulus aureus (syn. Malabaila aurea (Sm.) Boiss.) were examined by light and scanning electron microscopy in order to contribute to the taxonomical and melissopalynological studies of the species. Flower visitors have also been observed and analyzed aiming at clarifying some pollination aspects including the species contribution to the bee pasture. The pollen grains of L. aureus are isopolar, radially symmetrical, medium to large in size, tricolporate and perprolate. They are slightly equatorially constricted with obtuse polar caps and triangular in polar view. The sculpturing pattern is rugulate–microperforate. With regard to flower visitors, the following pollination types occurred: melittophily, myophily and sapromyophily and cantharophily. Some insects attracted by L. aureus cannot be considered pollinators but casual visitors. The flowers were the most frequently visited by honey bees during the midday.
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