Pub Date : 2022-01-03DOI: 10.37427/botcro-2021-026
A. Butiuc-Keul, A. Coste, H. Budahn, F. Dunemann, A. Farkas, D. Postolache, E. Klocke
Hypericum perforatum, H. umbellatum, H. maculatum, and H. hircinum accessions originating from botanical gardens across Europe were examined by flow cytometry and molecular markers. 2C DNA content of 17 Hypericum perforatum accessions (Hp) and the H. perforatum cultivar Topaz amounted to between 1.56 pg and 1.62 pg. In four Hp accessions some individual plants were found with a DNA content corresponding to 6Cx (2.34 - 2.39 pg). All plants of accession Hp8 showed a DNA content of 6Cx (2.41 pg). In root tips of Hp plants with an average DNA amount of 1.58 pg, 32 chromosomes were detected, corresponding to 2n = 4x. This is the first ploidy and/or DNA content report for H. umbellatum, H. maculatum and H. hircinum. H. umbellatum and H. maculatum, each contained 0.76 pg DNA and 16 chromosomes were counted. The 2C DNA content of H. hircinum was 1.00 pg with the best metaphase plate revealing 32 chromosomes. Additionally, a combined marker analysis, based on inter-simple sequence repeats (ISSR) and sequence related amplified polymorphism (SRAP), was conducted to gain a better understanding of diversity especially within the accessions of H. perforatum. A total of 27 (11 ISSR and 16 SRAP) primer combinations were screened, showing 699 bands, of which 661 were polymorphic. UPGMA clustering revealed that accessions from the same geographic area tended to be more closely related, while H. maculatum was grouped separately from all H. perforatum accessions. Both methods have shown similar sensitivities in detecting the genetic diversity of the analyzed genotypes. Our results may be useful for Hypericum breeding programs and the development of effective conservation strategies.
{"title":"Analysis of Hypericum accessions by DNA fingerprinting and flow cytometry","authors":"A. Butiuc-Keul, A. Coste, H. Budahn, F. Dunemann, A. Farkas, D. Postolache, E. Klocke","doi":"10.37427/botcro-2021-026","DOIUrl":"https://doi.org/10.37427/botcro-2021-026","url":null,"abstract":"Hypericum perforatum, H. umbellatum, H. maculatum, and H. hircinum accessions originating from botanical gardens across Europe were examined by flow cytometry and molecular markers. 2C DNA content of 17 Hypericum perforatum accessions (Hp) and the H. perforatum cultivar Topaz amounted to between 1.56 pg and 1.62 pg. In four Hp accessions some individual plants were found with a DNA content corresponding to 6Cx (2.34 - 2.39 pg). All plants of accession Hp8 showed a DNA content of 6Cx (2.41 pg). In root tips of Hp plants with an average DNA amount of 1.58 pg, 32 chromosomes were detected, corresponding to 2n = 4x. This\u0000is the first ploidy and/or DNA content report for H. umbellatum, H. maculatum and H. hircinum. H. umbellatum and H. maculatum, each contained 0.76 pg DNA and 16 chromosomes were counted. The 2C DNA content of H. hircinum was 1.00 pg with the best metaphase plate revealing 32 chromosomes. Additionally, a combined marker analysis, based on inter-simple sequence repeats (ISSR) and sequence related amplified polymorphism (SRAP), was conducted to gain a better understanding of diversity especially within the accessions of H. perforatum. A total of 27 (11 ISSR and 16 SRAP) primer combinations were\u0000screened, showing 699 bands, of which 661 were polymorphic. UPGMA clustering revealed that accessions from the same geographic area tended\u0000to be more closely related, while H. maculatum was grouped separately from all H. perforatum accessions. Both methods have shown similar sensitivities in detecting the genetic diversity of the analyzed genotypes. Our results may be useful for Hypericum breeding programs and\u0000the development of effective conservation strategies.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2022-01-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42718713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.37427/BOTCRO-2021-015
H. Eroğlu, M. Karaismailoğlu, S. M. Pinar, M. Fidan
This study presents the first in-depth evaluation of the morphological and anatomical characters, as well as their taxonomic importance, of the seeds of 36 taxa in subgenera Muscari, Leopoldia, Pseudomuscari and Botryanthus of the genus Muscari in Turkey, where 24 of the taxa are endemic. The results indicate that the taxa generally differ from each other in terms of seed shape and dimension. Seed dimensions vary between 1.66 mm and 3.21 mm in length, and between 1.12 mm and 2.63 mm in width. The seed surface ornamentation is grouped into nine forms: ruminate, reticulate, reticulate-areolate, reticulatefoveate, alveolate, scalariform, rugose, verrucate and areolate. The most common type is ruminate, while areolate, reticulate-foveate and scalariform ornamentation forms were found to be taxon-specific. Testa structures of the taxa examined consist in general of two different layers: the epidermis and the subepidermis in scleranchymatous or parenchymatous structures. The subepidermis may be absent in some of taxa. The structure and thickness of the epidermis and the subepidermis are very important characteristics that disclose interspecific relations among the examined taxa. We also provide a key for the identification of the studied taxa based on seed features.
{"title":"Seed micromorphology and anatomy of 36 Muscari (Asparagaceae) taxa from Turkey with notes on their systematic importance","authors":"H. Eroğlu, M. Karaismailoğlu, S. M. Pinar, M. Fidan","doi":"10.37427/BOTCRO-2021-015","DOIUrl":"https://doi.org/10.37427/BOTCRO-2021-015","url":null,"abstract":"This study presents the first in-depth evaluation of the morphological and anatomical characters, as well as their taxonomic importance, of the seeds of 36 taxa in subgenera Muscari, Leopoldia, Pseudomuscari and Botryanthus of the genus Muscari in Turkey, where 24 of the taxa are endemic. The results indicate that the taxa generally differ from each other in terms of seed shape and dimension. Seed dimensions vary between 1.66 mm and 3.21 mm in length, and between 1.12 mm and 2.63 mm in width. The seed surface ornamentation is grouped into nine forms: ruminate, reticulate, reticulate-areolate, reticulatefoveate, alveolate, scalariform, rugose, verrucate and areolate. The most common type is ruminate, while areolate, reticulate-foveate and scalariform ornamentation forms were found to be taxon-specific. Testa structures of the taxa examined consist in general of two different layers: the epidermis and the subepidermis in scleranchymatous or parenchymatous structures. The subepidermis may be absent in some of taxa. The structure and thickness of the epidermis and the subepidermis are very important characteristics that disclose interspecific relations among the examined taxa. We also provide a key for the identification of the studied taxa based on seed features.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":"0-0"},"PeriodicalIF":1.3,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44094119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.37427/BOTCRO-2021-016
Anita Dedić, D. Hafner, Anamarija Antunović, J. Kamberović, Svjetlana Stanić-Koštroman, M. Kelly
– The aims of this paper were to describe seasonal changes in the qualitative and quantitative composition of diatom taxa and the potential application of benthic diatoms for ecological status evaluation. Diatom indices (IPS and TI) were calculated from data from three different locations along a longitudinal profile of the Bunica, a small karstic river in Bosnia and Herzegovina. A total of 147 taxa were recorded in 12 samples. The most common taxa were Meridion circulare (Greville) C.Agardh and Ulnaria ulna (Nitzsch) Compère. Physical and chemical analyses showed low concentrations of nutrients, good oxygenation, typical pH for carbonate bed/origin and generally oligotrophic conditions and high ecological status. All sites had similar physico-chemical conditions and there were only few seasonal differences. Ordination of the diatom data showed that samples showed neither longitudinal nor seasonal patterns. Median value for IPS (16.8) and for TI (7.3) can be possible ‘‘expected’’ values for ecological status assessment for small karstic rivers in the Mediterranean region. We propose the use of the phytobenthos Intercalibration Common Metric (pICM - an index that combines the IPS and TI) as a national metric for countries developing WFD diatom methods at a late stage. One situation is described, and a solution, which is potentially transferable to other locations in Bosnia and Herzegovina and also to other countries facing similar challenges.
{"title":"Biodiversity and seasonal distribution of benthic diatom assemblages as an indicator of water quality of small karstic river in Bosnia and Herzegovina","authors":"Anita Dedić, D. Hafner, Anamarija Antunović, J. Kamberović, Svjetlana Stanić-Koštroman, M. Kelly","doi":"10.37427/BOTCRO-2021-016","DOIUrl":"https://doi.org/10.37427/BOTCRO-2021-016","url":null,"abstract":"– The aims of this paper were to describe seasonal changes in the qualitative and quantitative composition of diatom taxa and the potential application of benthic diatoms for ecological status evaluation. Diatom indices (IPS and TI) were calculated from data from three different locations along a longitudinal profile of the Bunica, a small karstic river in Bosnia and Herzegovina. A total of 147 taxa were recorded in 12 samples. The most common taxa were Meridion circulare (Greville) C.Agardh and Ulnaria ulna (Nitzsch) Compère. Physical and chemical analyses showed low concentrations of nutrients, good oxygenation, typical pH for carbonate bed/origin and generally oligotrophic conditions and high ecological status. All sites had similar physico-chemical conditions and there were only few seasonal differences. Ordination of the diatom data showed that samples showed neither longitudinal nor seasonal patterns. Median value for IPS (16.8) and for TI (7.3) can be possible ‘‘expected’’ values for ecological status assessment for small karstic rivers in the Mediterranean region. We propose the use of the phytobenthos Intercalibration Common Metric (pICM - an index that combines the IPS and TI) as a national metric for countries developing WFD diatom methods at a late stage. One situation is described, and a solution, which is potentially transferable to other locations in Bosnia and Herzegovina and also to other countries facing similar challenges.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47127424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.37427/BOTCRO-2021-014
K. Süveges, A. Molnár, A. Mesterházy, J. Budai, R. Fekete
– This paper reports the occurrence of a North American salt-tolerant taxon, Diplachne fusca subsp. fascicularis (Lam.) P.M.Peterson et N.Snow in Hungary (Central-Europe). Two earlier Hungarian observations of D. fusca were known from 1915, near Győr (West Transdanubia), later the taxon was collected by Pénzes in 1958, in downtown Budatétény (central Hungary. Both observations seem to be occasional. Recently, the taxon has started spreading in Europe, mainly on rice paddy fields, with a serious invasion potential. In North America its appearance on ruderal habitats, as well as along roads and other linear infrastructures is a well known phenomenon. The Hungarian population was found near Cegléd (Central Hungary) on the roadside of the E40 primary main road in September 2018. In July 2019 more than one thousand (mostly vegetative) individuals were detected. The salt content of the habitat shows remarkable temporal and spatial variability. At one meter distance from the edge of the paved road soil salt content was higher in spring (after the winter de-icing regime), than in autumn. Salt concentration was highest in the vicinity of the road, and decreased with increasing distance from it. Germination tests revealed a significant negative effect of NaCl concentration on germination rates, but germination occurred even on extremely saline substrates with 1.5% NaCl concentration. Considering its biology and reproduction strategy, the further spread of Diplachne fusca is highly presumable.
{"title":"Emergence of a new salt-tolerant alien grass along roadsides? Occurrence of Diplachne fusca subsp. fascicularis (Poaceae) in Hungary","authors":"K. Süveges, A. Molnár, A. Mesterházy, J. Budai, R. Fekete","doi":"10.37427/BOTCRO-2021-014","DOIUrl":"https://doi.org/10.37427/BOTCRO-2021-014","url":null,"abstract":"– This paper reports the occurrence of a North American salt-tolerant taxon, Diplachne fusca subsp. fascicularis (Lam.) P.M.Peterson et N.Snow in Hungary (Central-Europe). Two earlier Hungarian observations of D. fusca were known from 1915, near Győr (West Transdanubia), later the taxon was collected by Pénzes in 1958, in downtown Budatétény (central Hungary. Both observations seem to be occasional. Recently, the taxon has started spreading in Europe, mainly on rice paddy fields, with a serious invasion potential. In North America its appearance on ruderal habitats, as well as along roads and other linear infrastructures is a well known phenomenon. The Hungarian population was found near Cegléd (Central Hungary) on the roadside of the E40 primary main road in September 2018. In July 2019 more than one thousand (mostly vegetative) individuals were detected. The salt content of the habitat shows remarkable temporal and spatial variability. At one meter distance from the edge of the paved road soil salt content was higher in spring (after the winter de-icing regime), than in autumn. Salt concentration was highest in the vicinity of the road, and decreased with increasing distance from it. Germination tests revealed a significant negative effect of NaCl concentration on germination rates, but germination occurred even on extremely saline substrates with 1.5% NaCl concentration. Considering its biology and reproduction strategy, the further spread of Diplachne fusca is highly presumable.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45517206","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.37427/BOTCRO-2021-012
M. Ebadi, Rosa Eftekharian
– Senecio vulgaris L., an annual herb belonging to the Asteraceae, is widely distributed in different regions of the world. There is no information on the intraspecific variations of the morphological and molecular features of this species. In the present investigation, we studied the morphological and genetic diversity of 81 accessions of S. vulgaris collected from 10 geographical populations. Eleven inter simple sequence repeat (ISSR) primers were used for the examination of genetic variations among the populations. Analysis of molecular variance (AMOVA) and G ST analyses revealed significant differences among the investigated populations. A significant correlation between genetic distance and geographical distance was revealed by the Mantel test. However, reticulation analysis indicated the occurrence of gene flow among most of the populations studied. Principal component analysis (PCA) plot showed that the number of capitula, length of the cauline leaf and plant height were the most variable morphological characters. Principal coordinates analysis (PCoA) plot revealed two groups of populations, according to molecular and morphological data. The results suggested the existence of possible intraspecific taxonomic ranks within this species.
{"title":"Morphological and genetic diversity of Senecio vulgaris L. (Asteraceae) in Iran","authors":"M. Ebadi, Rosa Eftekharian","doi":"10.37427/BOTCRO-2021-012","DOIUrl":"https://doi.org/10.37427/BOTCRO-2021-012","url":null,"abstract":"– Senecio vulgaris L., an annual herb belonging to the Asteraceae, is widely distributed in different regions of the world. There is no information on the intraspecific variations of the morphological and molecular features of this species. In the present investigation, we studied the morphological and genetic diversity of 81 accessions of S. vulgaris collected from 10 geographical populations. Eleven inter simple sequence repeat (ISSR) primers were used for the examination of genetic variations among the populations. Analysis of molecular variance (AMOVA) and G ST analyses revealed significant differences among the investigated populations. A significant correlation between genetic distance and geographical distance was revealed by the Mantel test. However, reticulation analysis indicated the occurrence of gene flow among most of the populations studied. Principal component analysis (PCA) plot showed that the number of capitula, length of the cauline leaf and plant height were the most variable morphological characters. Principal coordinates analysis (PCoA) plot revealed two groups of populations, according to molecular and morphological data. The results suggested the existence of possible intraspecific taxonomic ranks within this species.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45086521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.37427/BOTCRO-2021-013
Mahmoud O. Hassan, Howida Y. Mohamed, A. H. Aboellil
– Pruning Ficus trees in urban green spaces may lead to the accumulation and spread of their leaf litter on the understory vegetation. This study was conducted to evaluate the allelopathic effect of Ficus retusa L. leaf litter on the understory species in urban gardens. A field study showed that the plant cover and species richness of litter-affected plots were lower than those of litter-free areas. The litter-affected soils had substantially lower pH and higher electrical conductivity. In a greenhouse experiment, litter-affected soil significantly inhibited the emergence and growth of understory species selected for the purpose of this study: Melilotus indicus (L.) All. , Trifolium resupinatum L. and Amaranthus viridis L. Osmotic potentials equivalent to those of the litter-affected soils did not affect emergence or growth of these species. A spectrophotometric analysis indicated that the litter-affected soils contained larger amounts of phenolics and flavonoids. An HPLC analysis revealed that the litter-affected soils contained higher concentrations of free phenolic and flavonoid allelochemicals. These results demonstrate that F . retusa leaf litter may reduce plant cover and species richness. The significant inhibition in both field and greenhouse experiments could be attributed to phenolic and flavonoid allelochemicals released from the tree litter, as the osmotic potential of the litter had no effect on the understory species. The allelopathic potential of F. retusa leaf litter plays at least a partial role in reducing urban vegetation.
{"title":"Allelopathic potential of Ficusretusa L. leaf litter on understory vegetation in urban gardens","authors":"Mahmoud O. Hassan, Howida Y. Mohamed, A. H. Aboellil","doi":"10.37427/BOTCRO-2021-013","DOIUrl":"https://doi.org/10.37427/BOTCRO-2021-013","url":null,"abstract":"– Pruning Ficus trees in urban green spaces may lead to the accumulation and spread of their leaf litter on the understory vegetation. This study was conducted to evaluate the allelopathic effect of Ficus retusa L. leaf litter on the understory species in urban gardens. A field study showed that the plant cover and species richness of litter-affected plots were lower than those of litter-free areas. The litter-affected soils had substantially lower pH and higher electrical conductivity. In a greenhouse experiment, litter-affected soil significantly inhibited the emergence and growth of understory species selected for the purpose of this study: Melilotus indicus (L.) All. , Trifolium resupinatum L. and Amaranthus viridis L. Osmotic potentials equivalent to those of the litter-affected soils did not affect emergence or growth of these species. A spectrophotometric analysis indicated that the litter-affected soils contained larger amounts of phenolics and flavonoids. An HPLC analysis revealed that the litter-affected soils contained higher concentrations of free phenolic and flavonoid allelochemicals. These results demonstrate that F . retusa leaf litter may reduce plant cover and species richness. The significant inhibition in both field and greenhouse experiments could be attributed to phenolic and flavonoid allelochemicals released from the tree litter, as the osmotic potential of the litter had no effect on the understory species. The allelopathic potential of F. retusa leaf litter plays at least a partial role in reducing urban vegetation.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"80 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43937965","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-16DOI: 10.37427/botcro-2021-025
D. Iamonico
Habrosia (Sagineae, Caryophyllaceae) is a genus that includes only H. spinuliflora, a species occurring in Iran, Iraq, Syria, Lebanon, and Turkey (Irano-Turanian floristic chorological element). Based on the available molecular data published in 2011, Habrosia appears to be nested in a Minuartia-clade, which includes taxa currently recognized under the genus Sabulina. Consequently, Habrosia should be treated as a genus to be included in Sabulina. However, the molecular tree published in 2011 considered only 9 Sabulina members whereas, according to the current concept, Sabulina is a genus comprising about 65 species. Unfortunately, the molecular phylogeny including a larger Sabulina sample published in 2014 did not include H. spinuliflora and the taxonomic position of Habrosia remains, therefore, uncertain. With the aim of verifying the correct position of Habrosia in the tribe Sagineae with respect to its relationship to Sabulina, a comprehensive molecular investigation based on ITS sequences, linked to detailed morphological data, is presented. The results obtained revealed that Habrosia is not part of Sabulina. A detailed description of H. spinuliflora, its ecological preference, and a distribution map are provided. Eventually, the name Arenaria spinulifolia (basionym of H. spinuliflora) is lectotypified on a specimen preserved at G (barcode G00212963).
{"title":"Habrosia (Caryophyllaceae) a monotypic genus endemic to Western Asia","authors":"D. Iamonico","doi":"10.37427/botcro-2021-025","DOIUrl":"https://doi.org/10.37427/botcro-2021-025","url":null,"abstract":"Habrosia (Sagineae, Caryophyllaceae) is a genus that includes only H. spinuliflora, a species occurring in Iran, Iraq, Syria, Lebanon, and Turkey (Irano-Turanian floristic chorological element). Based on the available molecular data published in 2011, Habrosia appears to be nested in a Minuartia-clade, which includes taxa currently recognized under the genus Sabulina. Consequently, Habrosia should be treated as a genus to be included in Sabulina. However, the molecular tree published in 2011 considered only 9 Sabulina members whereas, according to the current concept, Sabulina is a genus comprising about 65 species. Unfortunately, the molecular phylogeny including a larger Sabulina sample published in 2014 did not include H. spinuliflora and the taxonomic position of Habrosia remains, therefore, uncertain. With the aim of verifying the correct position of Habrosia in the tribe Sagineae with respect to its relationship to Sabulina, a comprehensive molecular investigation based on ITS sequences, linked to detailed morphological data, is presented. The results obtained revealed that Habrosia is not part of Sabulina. A detailed description of H. spinuliflora, its ecological preference, and a distribution map are provided. Eventually, the name Arenaria spinulifolia (basionym of H. spinuliflora) is lectotypified on a specimen preserved at G (barcode G00212963).","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44900821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-16DOI: 10.37427/botcro-2021-022
A. Shahzad, Mingzhou Qin, Mudassar Nazir, A. Shakoor, Motsim Billah, G. Zaib
Anthropogenic activities have added a large amount of heavy metals to the environment. Heavy metal contaminants affect the physiological and biological properties of soil and plant health. Zinc (Zn) is an essential micronutrient and it promotes plant growth and development but a higher concentration of the metal causes reduction in plant growth. The present study was aimed to evaluate the response of Bacillus cereus on maize plants at different concentrations of ZnSO4 (20, 40 and 60 mg kg–1) amended in the soil under pot experiment conditions. The experiment was conducted by using complete randomized design (CRD) with three replications. Higher doses of ZnSO4 inhibited maize growth and nutrient uptake. However, inoculation of maize seeds with Bacillus cereus at 20 mg kg–1 concentration of ZnSO4 increased seed germination about 39% and plant height by 15%. Moreover, 17% increase in leaf length and a 7% increase in leaf number were observed as compared to control at 20 mg kg–1 concentrations of ZnSO4. Reductions in all growth parameters were observed with 60 mg kg–1 concentration of ZnSO4. The Zn uptake was 75% higher in treatment T8 (uninoculated seeds with 60 mg kg–1 concentration of ZnSO4) as compared to treatments which were inoculated and grown under different zinc concentrations. The results suggest that Bacillus cereus has good potential to remediate Zn from soil as well as to reduce the phyto-availibility and phytotoxicity of zinc.
{"title":"Response of Bacillus cereus on Zea mays under different doses of zinc sulphate","authors":"A. Shahzad, Mingzhou Qin, Mudassar Nazir, A. Shakoor, Motsim Billah, G. Zaib","doi":"10.37427/botcro-2021-022","DOIUrl":"https://doi.org/10.37427/botcro-2021-022","url":null,"abstract":"Anthropogenic activities have added a large amount of heavy metals to the environment. Heavy metal contaminants affect the physiological and biological properties of soil and plant health. Zinc (Zn) is an essential micronutrient and it promotes plant growth and development but\u0000a higher concentration of the metal causes reduction in plant growth. The present study was aimed to evaluate the response of Bacillus cereus on maize plants at different concentrations of ZnSO4 (20, 40 and 60 mg kg–1) amended in the soil under pot experiment conditions. The experiment was conducted by using complete randomized design (CRD) with three replications. Higher doses of ZnSO4 inhibited maize growth and nutrient uptake. However, inoculation of maize seeds with Bacillus cereus at 20 mg kg–1 concentration of ZnSO4 increased seed germination about 39% and plant height by 15%. Moreover, 17% increase in leaf length\u0000and a 7% increase in leaf number were observed as compared to control at 20 mg kg–1 concentrations of ZnSO4. Reductions in all growth parameters were observed with 60 mg kg–1 concentration of ZnSO4. The Zn uptake was 75% higher in treatment T8 (uninoculated seeds with 60 mg kg–1 concentration of ZnSO4) as compared to treatments which were inoculated and grown under different zinc concentrations. The results suggest that Bacillus cereus has good potential to remediate Zn from soil as well as to reduce the phyto-availibility and phytotoxicity of zinc.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49615122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-16DOI: 10.37427/botcro-2021-024
E. Kaya, Sevil Yeniocak, İrem Aktay, O. Çelįk, Bilge Ozturk, Damla Ekin Ozkaya, Sevinc Guldag, Taner Mercan, S. Galatali, O. Akguller, Mehmet Ali Balci
Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.
纤毛胸腺是东地中海的一种特有元素，具有芳香的药用特性。它的自然种群分布在安纳托利亚南部和西南部的砾石地面和开阔的岩石地区。目前对弯孢霉体外繁殖的研究主要集中在环境应用方面，如开发高效和遗传稳定的微繁殖的最佳培养基组成，以及改进保存程序以长期保存优良种质以供进一步研究。为此，单独使用MS和OM培养基，并与细胞分裂素、木炭、AgNO3、Fe-EDDHA和H3BO3组合使用。通过图核方法对原始数据进行统计分析，以优化所有参数之间的非线性关系。以10 g L-1活性炭和1 mg L-1 KIN为复合培养基，优化了毛滴虫的增殖培养基，在该培养基上计算出的最佳再生率、最佳芽数和最佳芽长的平均值分别为96.89%、3和1.24。利用ISSR-PCR技术，在用图形核法获得的最佳培养基组成上测定了离体植物的遗传稳定性。所有ISSR引物共产生192个繁殖带谱，没有一个是多态性的。此外，微繁殖植物成功生根并适应温室条件。在这项研究中，我们提出了一个图核多重传播指数，它考虑了所有可能需要分析的参数。这种指数首次用于确定最佳增殖培养基。
{"title":"Development of an optimum proliferation medium via the graph kernel statistical analysis method for genetically stable in vitro propagation of endemic Thymus cilicicus (Turkey)","authors":"E. Kaya, Sevil Yeniocak, İrem Aktay, O. Çelįk, Bilge Ozturk, Damla Ekin Ozkaya, Sevinc Guldag, Taner Mercan, S. Galatali, O. Akguller, Mehmet Ali Balci","doi":"10.37427/botcro-2021-024","DOIUrl":"https://doi.org/10.37427/botcro-2021-024","url":null,"abstract":"Thymus cilicicus is an endemic Eastern Mediterranean element that has aromatic-medicinal properties. Its natural population spreads across gravelly ground and open rocky areas of South and Southwest Anatolia. The current study on in vitro propagation of T. cilicicus focused deeply\u0000on environmental applications such as the development of an optimum medium composition for efficient and genetically stable micropropagation\u0000and improved preservation procedures for long-time conservation of elite germplasms for further studies. For this purpose, MS and OM media were used individually and in combination with cytokinins, charcoal, AgNO3, Fe-EDDHA, and H3BO3. The raw data were statistically analyzed via\u0000the graph kernel method to optimize the nonlinear relationship between all parameters. The optimal proliferation medium for T. cilicicus was OM\u0000supplemented with a combination of 10 g L-1 charcoal and 1 mg L-1 KIN and the calculated averages of the best regeneration rate, the best shoot number and the best shoot length were 96.89%, 3 and 1.24 respectively on this medium. The determination of genetic stability of in vitro grown plants on the optimum medium compositions obtained by the\u0000graph kernel method was carried out with the use of the ISSR-PCR technique. All the ISSR primers produced a total of 192 reproductive band profiles, none of which were polymorphic. Furthermore, the micropropagated plants were successfully rooted and acclimatized to greenhouse conditions. In this study, we present a graph kernel multiple\u0000propagation index which considers all the possible parameters needing to be analyzed. Such an index is used for the first time for the determination of the optimum proliferation medium.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42622994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-09-16DOI: 10.37427/botcro-2021-023
M. Cerrato, A. Ribas-Serra, C. Cardona, L. Gil
Based on plant material collected in the forest nursery of the Balearic Island Forestry Center (CEFOR) for autochthonous plant production and the University of the Balearic Islands experimental facilities, two new plant records are presented for the Mediterranean island of Mallorca. Dactyloctenium aegyptium, an invasive grass previously recorded in other areas of the Mediterranean basin, and Glinus oppositifolius, a new record for the European flora. In both cases the species are presumed to have arrived through contaminated batches of the coconut fibre substrate used in both facilities.
{"title":"Species introductions through coconut fibre: Dactyloctenium aegyptium and Glinus oppositifolius, new records for the Balearic Islands, Spain","authors":"M. Cerrato, A. Ribas-Serra, C. Cardona, L. Gil","doi":"10.37427/botcro-2021-023","DOIUrl":"https://doi.org/10.37427/botcro-2021-023","url":null,"abstract":"Based on plant material collected in the forest nursery of the Balearic Island Forestry Center (CEFOR) for autochthonous plant production and the University of the Balearic Islands experimental facilities, two new plant records are presented for the Mediterranean island of Mallorca. Dactyloctenium aegyptium, an invasive grass previously recorded in other\u0000areas of the Mediterranean basin, and Glinus oppositifolius, a new record for the European flora. In both cases the species are presumed to\u0000have arrived through contaminated batches of the coconut fibre substrate used in both facilities.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2021-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42912429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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