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Optimization of solid state fermentation of sugar cane by aspergillus niger considering particles size effect 考虑粒径效应的黑曲霉固态发酵甘蔗的优化研究
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110106
J. Echevarria, J. A. R. León, M. E. Espinosa, G. Delgado
The protein enrichment of sugar cane by solid state fermentation employing Aspergillus niger was optimized in a packed bed column using a two Factor Central Composit Design α = 2, considering as independent factors the particles diameter corresponding to different times of grinding for a sample and the air flow rate. It was significative for the air flow rate (optimum 4.34 VKgM) and the particle diameter (optimum 0.136 cm). The average particle size distribution, shape factor, specific surface, volume-surface mean diameter, number of particles, real and apparent density and hollowness for the different times of grinding were determined, in order to characterize the samples.
采用两因素中心组合设计(α = 2),以不同磨粒次数对应的颗粒直径和空气流速为独立因素,在填充床柱上优化了黑曲霉固态发酵甘蔗的蛋白质富集。对空气流速(最优4.34 VKgM)和颗粒直径(最优0.136 cm)均有显著影响。测定不同磨削时间的平均粒度分布、形状因子、比表面积、体面平均直径、颗粒数、实密度、表观密度和空心度,以表征样品的特性。
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引用次数: 9
Lebensmittelmikrobiologie. Grundlagen für die Praxis (2. überarb. und em. Auflage). Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong: Springer‐Verlag, 1989. 253 pp., 79 Fig., 36 Tab., 189 Lit., DM 59,– ISBN 3‐540‐50285‐8 Lebensmittelmikrobiologie .什么是“实践基础”(2)überarb .反腐化。柏林、海德堡、纽约、伦敦、巴黎、东京、香港:马‐出版社,1989 .253章,79格36章189个,Lit . 59,书3马克‐540‐50285‐8
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110103
K. Sattler
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引用次数: 1
Energy balance in solid state fermentation processes 固态发酵过程中的能量平衡
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110104
Rodriguez Leön, J. A. Torres, J. Echevarrí, G. Saura
It was applied a macroscopic energy balance to a solid state fermentation process and an electron balance in order to estimate the temperature and the heat evolved in the process. There were employed several equations that describe the development of the system and offer the possibility to design or control such fermentations.
将宏观能量平衡法应用于固体发酵过程,并用电子平衡法估算发酵过程中的温度和热量。有几个方程描述了系统的发展,并提供了设计或控制这种发酵的可能性。
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引用次数: 26
Bioflavour'87. Analysis · Biochemistry · Biotechnology, Proceedings of the International Conference, Würzburg, Federal Republic of Germany, September 29 – 30,1987. Berlin, New York: Walter de Gryter, 1988. 584 pp., DM 318,– ISBN 3‐11‐01 1204‐3 Bioflavour 87年。分析·生物化学·生物技术,国际会议论文集,德国联邦共和国,1987年9月29 - 30日。柏林,纽约:Walter de Gryter, 1988。584页,DM 318, - ISBN 3‐11‐01 1204‐3
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110108
J. Schneider
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引用次数: 0
Yeast Technology. Berlin, Heidelberg, New York, London, Paris, Tokyo, Hong Kong: Springer-Verlag, 1989. 407 pp., 54 Fig., DM 198,- ISBN 3-540-50689 酵母技术。柏林、海德堡、纽约、伦敦、巴黎、东京、香港:斯普林格出版社,1989。407页,54图,DM 198,- ISBN 3-540-50689
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110105
K. Triems
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引用次数: 0
Isolation and utilization of indigenous yeast strain for brewing of millet beer (OYOKPO) 国产小米啤酒酵母菌株的分离与利用
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110109
N. Uraih, W. E. Anetekhai
Three yeast strains were isolated from a spontaneously fermented native millet (Pennisetum typhoideum) malt beer (Oyokpo). One of the yeast isolates found to have the most highly fermenting capacity was characterised and identified as a strain of Saccharomyces cerevisiae. The yeast was then utilised as the pitching yeast in a subsequent controlled fermentation of millet wort at 20°C for 120 hours. Bitter leaf (Vernonia amagdalina) extract was used as the bittering and flavouring agent. The Oyokpo beer sample produced under these conditions was found to possess both chemical and organoleptic qualities comparable to some extent, to the conventional barley malt beer. At the end of fermentation, the pH, specific gravity, alcohol content, reducing sugar content and protein content of the beer were 4.11, 1.0308, 2.81% (v/v), 4.00 (mg/ml) and 0.84 (mg/ml) respectively.
从一种天然发酵的本地小米(Pennisetum typhoideum)麦芽啤酒(Oyokpo)中分离出3株酵母菌。其中一株发现具有最高发酵能力的酵母菌被鉴定为酿酒酵母菌株。然后将该酵母作为俯仰酵母,在随后的20°C下控制发酵小米麦芽汁120小时。以苦叶提取物为苦味剂和调味剂。在这些条件下生产的Oyokpo啤酒样品在某种程度上具有与传统大麦芽啤酒相当的化学和感官品质。发酵结束时,啤酒的pH值为4.11,比重为1.0308,酒精含量为2.81% (v/v),还原糖含量为4.00 (mg/ml),蛋白质含量为0.84 (mg/ml)。
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引用次数: 2
The application of linear irreversible thermodynamics to biological growth 线性不可逆热力学在生物生长中的应用
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110102
F. Baumann
We measured the rate of cell biomass growth, the consumption of organic substrate, and the heat developed during growth. The relationship between thermodynamic efficiency and the rate of microbial growth was considered.
我们测量了细胞生物量的生长速率、有机基质的消耗以及生长过程中产生的热量。考虑了热力学效率与微生物生长速率之间的关系。
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引用次数: 3
Introduction of the tac-promoter by lactose under fermentation conditions 在发酵条件下引入乳糖促进剂
Pub Date : 1991-01-01 DOI: 10.1002/ABIO.370110107
P. Neubauer, C. Wolff, M. Hecker, K. Hofmann, L. Meyer, P. Kruschke, H. Heinrich
The use of the expensive IPTG for induction of the tac-promoter is not practicable at the industrial scale because of the large amounts necessary for induction. We developed a system in which IPTG as inducer was replaced by lactose which induces the tac-promoter with the same efficiency. In E. coli CW3011 (pOF1.0) lactose is utilized as inducer and carbon source even in the presence of glucose, presumably as a consequence of the overproduction of lac-permease.
使用昂贵的IPTG来诱导tac启动子在工业规模上是不可行的,因为诱导所需的量很大。我们开发了一个用乳糖代替IPTG作为诱导剂诱导tac启动子的系统,其诱导效率相同。在大肠杆菌CW3011 (pOF1.0)中,即使在葡萄糖存在的情况下,乳糖也被用作诱导剂和碳源,可能是由于渗透酶过量产生的结果。
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引用次数: 17
Oil feeding during the oxytetracycline biosynthesis 土霉素生物合成过程中油的摄食
Pub Date : 1987-01-01 DOI: 10.1002/ABIO.370070102
P. Ettler
The ability of utilization of vegetal oils belongs to common properties of Streptomycetes. During the cultivation of Streptomyces rimosus and biosynthesis of oxytetracycline in 15 m3 fermenters, a fixed regime of soya bean oil feeds as antifoam agent and partial carbon source according to subjectively designed mechanism was investigated and further also feeding regime after exhausting starch as main carbon source and finally his dosing with ammonia sulphate in accordance with physiological activity of the culture. In the second case with semicontinuous supplements of soya bean oil it was possible to prevent premature autolysis of mycelia and to extend the biosynthetic process with keeping the oxytetracycline production rate. The third approach, the most progressive one, represents besides the automatisation of control loop the necessity of assurance continuous pH adjustment for elevation of the process production rate.
利用植物油的能力是链霉菌的共同特性。在15 m3发酵罐中进行链霉菌的培养和土霉素的生物合成过程中,根据主观设计的机理,研究了豆油作为消泡剂和部分碳源的固定饲喂制度,并根据培养物的生理活性,进一步研究了以淀粉为主要碳源,再添加硫酸铵的饲喂制度。在第二种情况下,半连续补充大豆油有可能防止菌丝体过早自溶,并在保持土霉素产量的情况下延长生物合成过程。第三种方法是最先进的方法,除了控制回路的自动化之外,还代表了保证连续调整pH值以提高工艺生产率的必要性。
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引用次数: 6
Nutzung cellulosehaltiger Abprodukte bei der Biosynthese von Cellulasen 利用废糖体生成素产生蜂巢组织
Pub Date : 1987-01-01 DOI: 10.1002/abio.370070518
D. G. Pobedimskij, I. E. Mukmenev, V. I. Savdur, A. G. Liakumovic, S. I. Agadganjan
It was analysed the influence of different pretreatment methods of cellulosic materials to increase the productivity of the cellulase biosynthesis. The most effective pretreatment method for cotton cellulose is the thermomechanical destruction in a worm extruder (5–15 minutes) with hydrolysis catalysts. The enzymatic saccharification degree was increased about 4 fold. Utilizing a pretreated cellulose an increase of 70–120% in C1-activity was found besides their level was stabilized in higher values. It was demonstrated that enzymatic hydrolysis parameters would be applied as model for biochemical utilization of cellulosic materials in the biosynthesis of cellulases.
分析了纤维素原料不同预处理方法对提高纤维素酶生物合成效率的影响。棉纤维素最有效的预处理方法是用水解催化剂在蠕虫挤出机中(5-15分钟)进行热机械破坏。酶促糖化程度提高约4倍。经预处理后的纤维素的c1活性提高了70-120%,并稳定在较高的水平。结果表明,酶解参数可作为纤维素原料在纤维素酶生物合成中的生化利用模型。
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引用次数: 0
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