Microchimica Acta最新文献

英文中文

Simultaneous detection and removal of tetracycline antibiotics in water using silver-based metal-organic frameworks

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-10 DOI: 10.1007/s00604-025-07000-8

Yajing Cui, Qiulin Li, Junchao Qian, Feng Chen

Tetracyclines (TCs), a class of broad-spectrum antibiotics, are major contaminants in water, have adverse effects on the ecosystem, and are toxic non-target organisms. A straightforward and efficient strategy for both the detection and removal of TCs from water remains highly desirable but is challenging to develop. In this study, a dual-functional platform for detecting and removing TCs was developed using highly stable silver-based metal-organic frameworks (Ag-MOFs). This platform enabled the specific detection of TCs over a broad concentration range (from 1 × 10⁻¹⁰ to 1 × 10⁻³ mol/L), with a low detection limit of 8.4 nM. By leveraging its high surface area, the Ag-MOFs exhibited exceptional adsorption capacities, reaching 276 mg/g for chlortetracycline. To elucidate the potential response mechanism and electronic transfer pathway, density functional theory calculations and charge density difference analyses were performed. This Ag-MOFs-based platform achieved both rapid detection and efficient removal of TCs from the environment. The design principles proposed herein are expected to inspire the development of novel platforms for the simultaneous sensing and removal of specific pollutants.

Graphical Abstract

{"title":"Simultaneous detection and removal of tetracycline antibiotics in water using silver-based metal-organic frameworks","authors":"Yajing Cui, Qiulin Li, Junchao Qian, Feng Chen","doi":"10.1007/s00604-025-07000-8","DOIUrl":"10.1007/s00604-025-07000-8","url":null,"abstract":"<div><p>Tetracyclines (TCs), a class of broad-spectrum antibiotics, are major contaminants in water, have adverse effects on the ecosystem, and are toxic non-target organisms. A straightforward and efficient strategy for both the detection and removal of TCs from water remains highly desirable but is challenging to develop. In this study, a dual-functional platform for detecting and removing TCs was developed using highly stable silver-based metal-organic frameworks (Ag-MOFs). This platform enabled the specific detection of TCs over a broad concentration range (from 1 × 10⁻<sup>10</sup> to 1 × 10⁻<sup>3</sup> mol/L), with a low detection limit of 8.4 nM. By leveraging its high surface area, the Ag-MOFs exhibited exceptional adsorption capacities, reaching 276 mg/g for chlortetracycline. To elucidate the potential response mechanism and electronic transfer pathway, density functional theory calculations and charge density difference analyses were performed. This Ag-MOFs-based platform achieved both rapid detection and efficient removal of TCs from the environment. The design principles proposed herein are expected to inspire the development of novel platforms for the simultaneous sensing and removal of specific pollutants.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379830","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-performance Ag-modified ZnO coral-like nanosheets for enhanced ethanol gas sensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-10 DOI: 10.1007/s00604-025-07013-3

Leyao Bi, Boyan Fu, Jiahui Lin, Junsheng Wen, Jiaxing Fu, Binglin Zou, Ningning Su, Chunjie Wang, Yue Wang

Ag-modified ZnO coral-like nanosheets were synthesized for the first time using a simple hydrothermal method. Compared to other Ag-modified ZnO sensors, the sensor with 2 wt% Ag content demonstrates excellent ethanol-sensing performance. The maximum response value of 67.42 can be achieved at the optimal operating temperature of 260 °C for 100 ppm ethanol gas, with corresponding response/recovery time of 6/75 s, respectively. This sensor also exhibits superior selectivity, reproducibility, and long-term stability. Techniques such as XRD, XPS, SEM, and TEM were employed to characterize the physicochemical properties of the gas-sensitive materials. Further research indicates that the enhanced gas-sensing properties of the composite materials can be mainly attributed to the unique coral-like nanosheet structure, the relatively high-specific surface area (9.8706 m²/g), and the combined effects of the Ag-ZnO heterostructure, as well as the synergistic interplay of electronic sensitization and spillover effects. This research offers valuable insights into the modification of ZnO-based gas-sensing materials with noble metals.

Graphical Abstract

{"title":"High-performance Ag-modified ZnO coral-like nanosheets for enhanced ethanol gas sensing","authors":"Leyao Bi, Boyan Fu, Jiahui Lin, Junsheng Wen, Jiaxing Fu, Binglin Zou, Ningning Su, Chunjie Wang, Yue Wang","doi":"10.1007/s00604-025-07013-3","DOIUrl":"10.1007/s00604-025-07013-3","url":null,"abstract":"<div><p> Ag-modified ZnO coral-like nanosheets were synthesized for the first time using a simple hydrothermal method. Compared to other Ag-modified ZnO sensors, the sensor with 2 wt% Ag content demonstrates excellent ethanol-sensing performance. The maximum response value of 67.42 can be achieved at the optimal operating temperature of 260 °C for 100 ppm ethanol gas, with corresponding response/recovery time of 6/75 s, respectively. This sensor also exhibits superior selectivity, reproducibility, and long-term stability. Techniques such as XRD, XPS, SEM, and TEM were employed to characterize the physicochemical properties of the gas-sensitive materials. Further research indicates that the enhanced gas-sensing properties of the composite materials can be mainly attributed to the unique coral-like nanosheet structure, the relatively high-specific surface area (9.8706 m<sup>2</sup>/g), and the combined effects of the Ag-ZnO heterostructure, as well as the synergistic interplay of electronic sensitization and spillover effects. This research offers valuable insights into the modification of ZnO-based gas-sensing materials with noble metals.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143379829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Development of a molecularly imprinted electrochemical sensor for erlotinib detection using a modified screen-printed carbon electrode

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-10 DOI: 10.1007/s00604-025-07012-4

Saif Ali Ahmed, Mahmoud Roushani, Zahra Mirzaei Karazan

A screen-printed carbon electrode (SPCE) based on a molecularly imprinted polymer (MIP) technique has been modified to provide detection of erlotinib (ERL). For this purpose, the pyrogallol (PG) monomer in the presence of ERL as an analyte has been electropolymerized on the SPCE surface. The introduced sensor has been characterized through differential pulse voltammetry (DPV), cyclic voltammetry (CV), and field emission scanning electron microscopy (FESEM) methods. The modified SPCE allowed ERL detection in a linear range of 0.05–800 nM with a limit of detection (LOD) of 0.016 nM. The applicability of modified SPCE was successfully investigated in serum samples. Lastly, for validation, sensor performance was compared with high-performance liquid chromatography (HPLC). The introduced sensor exhibited good application in the biomedical field.

Graphical Abstract

引用次数: 0

Formation of anisotropic nanoparticle structure for nanoplasmonic biosensing

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-08 DOI: 10.1007/s00604-025-06998-1

Chaoshan Zhao, Minshan Gan, Zhuoya Jiang, Zilan Lv, Wei Feng, Li Wang, Shunbo Li

A novel anisotropic nanoparticle structure consisting of a single gold nanorod and nanospheres is designed. The optical properties, especially the scattering under polarized light, are studied. The signal readout is conducted using dark-field microimaging techniques in a microfluidic chip. The formation of this novel structure is induced by the intermediate biomolecules. Therefore, it demonstrates potential applications in the ultrasensitive detection of biomarkers. As an example, the detection of vascular endothelial growth factor (VEGF165) is demonstrated, and the specificity is also investigated. This unique approach not only effectively reduces background interference but also provides a new approach for accurate sensing of targeted tumor markers.

Graphical Abstract

引用次数: 0

A programmable paper-based microfluidic chip based on multicolor fluorescence carbon dots for visual sensing of multiple catecholamines in human serum 基于多色荧光碳点的可编程纸基微流控芯片，用于目测人血清中的多种儿茶酚胺类物质

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-08 DOI: 10.1007/s00604-025-07017-z

Jiajing Zhang, Jia An, Yaqin Han, Junan Fang, Yufei Liu

Catecholamines (epinephrine, norepinephrine, and levodopa) are a kind of neurotransmitter with similar structure, which orchestrates the regulation of emotional balance, stress adaptation, cardiovascular system optimization, and energy metabolism coordination. To satisfy the actual requirements for rapid, accurate, and on-site detection of multiple catecholamines, we propose a programmable paper-based microfluidic chip, which is loaded with diethanolamine as a multifunctional and efficient non-fluorescent detection probe. Specifically, diethanolamine reacts with epinephrine, norepinephrine, and levodopa to generate polymer carbon dots with yellow, green, and blue fluorescence. On this basis, the integration of paper-based microfluidics with smartphone-assisted technology enables the intelligent visual fluorescence detection of multiple catecholamines. The analytical device exhibits a good linear relationship in the detection of epinephrine (0.008 ~ 0.5 mM), norepinephrine (0 ~ 0.2 mM), and levodopa (0.004 ~ 0.06 mM), with lower limits of detection of 7.4, 19.6, and 1.6 μM, respectively. The practicability and efficiency of the analytical detection device were further demonstrated through the analysis of human serum samples. The intelligent analytical device for the individual quantitative detection and visual differentiation of epinephrine, norepinephrine, and levodopa provides a novel and exciting avenue for accurate detection of multiple catecholamines.

Graphical Abstract

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引用次数: 0

Fluorine-fluorine interaction-driven colorimetric sensor for PFOA-sensitive detection using F-functionalized Ce-UiO-66-NH2 MOF with oxidase-like activity

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-06 DOI: 10.1007/s00604-025-07014-2

Nairong Yang, Xinyu Lin, Xiaomei Zheng, Wenqiang Lai, Youxiu Lin, Zehua Zou, Qingxiang Wang, Xuan Zheng

A novel colorimetric sensor was designed for sensitive perfluorooctanoic acid (PFOA) detection based on a fluorine-functionalized Ce-metal–organic framework (F-Ce-UiO-66-NH₂) with oxidase-like activity, using 3,3′,5,5′-tetramethylbenzidine (TMB) as the chromogenic substrate. This F-Ce-UiO-66-NH₂ was synthesized through ligand exchange and post-modification with pentafluorobenzaldehyde (PFBA) on the basis of Ce-terephthalic acid (Ce-UiO-66), incorporating pentafluorophenyl groups that enhance the material’s affinity for PFOA, leading to a more sensitive absorbance change in the presence of PFOA. Experimental and computational assays revealed that oxidase-like activity of F-Ce-UiO-66-NH₂ primarily arises from hydroxyl radicals (•OH) generated through the conversion of superoxide radicals (•O₂⁻). Furthermore, PFOA molecules were shown to undergo self-aggregation on the F-Ce-UiO-66-NH₂ surface via fluorine-fluorine (F-F) interactions between PFOA molecules and the pentafluorophenyl groups as well as between PFOA themselves, blocking the active Ce sites and hindering the interaction of O₂ and TMB with F-Ce-UiO-66-NH₂, thereby diminishing its oxidase-like activity. Owing to these sophisticated mechanisms, this colorimetric sensor demonstrated a broad linear detection range from 0.5 to 210 µM with a low detection limit of 0.41 µM for PFOA, enabling precise quantification of PFOA concentrations in real environmental water samples. This work introduces a new strategy for constructing field-deployable colorimetric sensors based on F-F interaction, offering very valuable insights into the design and operational principle for PFAS detection.

Graphical Abstract

{"title":"Fluorine-fluorine interaction-driven colorimetric sensor for PFOA-sensitive detection using F-functionalized Ce-UiO-66-NH2 MOF with oxidase-like activity","authors":"Nairong Yang, Xinyu Lin, Xiaomei Zheng, Wenqiang Lai, Youxiu Lin, Zehua Zou, Qingxiang Wang, Xuan Zheng","doi":"10.1007/s00604-025-07014-2","DOIUrl":"10.1007/s00604-025-07014-2","url":null,"abstract":"<div><p> A novel colorimetric sensor was designed for sensitive perfluorooctanoic acid (PFOA) detection based on a fluorine-functionalized Ce-metal–organic framework (F-Ce-UiO-66-NH<sub>2</sub>) with oxidase-like activity, using 3,3′,5,5′-tetramethylbenzidine (TMB) as the chromogenic substrate. This F-Ce-UiO-66-NH<sub>2</sub> was synthesized through ligand exchange and post-modification with pentafluorobenzaldehyde (PFBA) on the basis of Ce-terephthalic acid (Ce-UiO-66), incorporating pentafluorophenyl groups that enhance the material’s affinity for PFOA, leading to a more sensitive absorbance change in the presence of PFOA. Experimental and computational assays revealed that oxidase-like activity of F-Ce-UiO-66-NH<sub>2</sub> primarily arises from hydroxyl radicals (•OH) generated through the conversion of superoxide radicals (•O<sub>2</sub><sup>−</sup>). Furthermore, PFOA molecules were shown to undergo self-aggregation on the F-Ce-UiO-66-NH<sub>2</sub> surface via fluorine-fluorine (F-F) interactions between PFOA molecules and the pentafluorophenyl groups as well as between PFOA themselves, blocking the active Ce sites and hindering the interaction of O<sub>2</sub> and TMB with F-Ce-UiO-66-NH<sub>2</sub>, thereby diminishing its oxidase-like activity. Owing to these sophisticated mechanisms, this colorimetric sensor demonstrated a broad linear detection range from 0.5 to 210 µM with a low detection limit of 0.41 µM for PFOA, enabling precise quantification of PFOA concentrations in real environmental water samples. This work introduces a new strategy for constructing field-deployable colorimetric sensors based on F-F interaction, offering very valuable insights into the design and operational principle for PFAS detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143254453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A mini-review on the research progress and application of nanomaterials in electrochemiluminescent sensors in the detection of water environmental pollutants

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-04 DOI: 10.1007/s00604-025-06973-w

Longmei Yao, Jiajia Zhi, Wenchang Wang, Qingyi Li, Ding Jiang, Xiaohui Chen, Zhidong Chen

With the increasingly serious problem of environmental pollution, the development of new and efficient detection technology has become an urgent need. Electrochemiluminescence (ECL) sensors have attracted wide attention in environmental pollution detection due to their advantages of low cost, fast analysis speed, high sensitivity, and good selectivity. At the same time, with the rapid development of nanotechnology, nanomaterials are widely used to construct ECL sensors. Based on the different roles of nanomaterials in the construction of ECL sensors, they can be summarized as (1) nanomaterials for signal amplification; (2) ECL nanoemitters; (3) Nanomaterials as receptors for ECL resonance energy transfer. In this paper, the construction and luminescence mechanism of ECL sensors are discussed from the above three aspects. Finally, the challenges and prospects of nanomaterials ECL sensors in the field of environmental pollution detection in the future are discussed.

Graphical abstract

{"title":"A mini-review on the research progress and application of nanomaterials in electrochemiluminescent sensors in the detection of water environmental pollutants","authors":"Longmei Yao, Jiajia Zhi, Wenchang Wang, Qingyi Li, Ding Jiang, Xiaohui Chen, Zhidong Chen","doi":"10.1007/s00604-025-06973-w","DOIUrl":"10.1007/s00604-025-06973-w","url":null,"abstract":"<div><p>With the increasingly serious problem of environmental pollution, the development of new and efficient detection technology has become an urgent need. Electrochemiluminescence (ECL) sensors have attracted wide attention in environmental pollution detection due to their advantages of low cost, fast analysis speed, high sensitivity, and good selectivity. At the same time, with the rapid development of nanotechnology, nanomaterials are widely used to construct ECL sensors. Based on the different roles of nanomaterials in the construction of ECL sensors, they can be summarized as (1) nanomaterials for signal amplification; (2) ECL nanoemitters; (3) Nanomaterials as receptors for ECL resonance energy transfer. In this paper, the construction and luminescence mechanism of ECL sensors are discussed from the above three aspects. Finally, the challenges and prospects of nanomaterials ECL sensors in the field of environmental pollution detection in the future are discussed.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-06973-w.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic separations in chemistry and life sciences

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-04 DOI: 10.1007/s00604-025-06980-x

Fotios Tsopelas, Chrysanthos Stergiopoulos, Panagiotis Danias, Anna Tsantili-Kakoulidou

Since Otto Schmitt introduced the term “biomimetics” in 1957, the imitation of biological systems to develop separation methods and simulate biological processes has seen continuous growth, particularly over the past five decades. The biomimetic approach relies on the use of specific ligands—biospecific, biomimetic, or synthetic—which target biomolecules, such as proteins, antibodies, nucleic acids, enzymes, drugs, pesticides, and other bioactive analytes. This review highlights advances in biomimetic separations, focusing on biomimetic liquid chromatography (including immobilized artificial membrane chromatography, cell membrane chromatography, biomimetic affinity chromatography, weak affinity chromatography, micellar liquid chromatography, immobilized liposome chromatography, and liposome electrokinetic capillary chromatography) for the complex separation and purification of biomolecules and other important chemical compounds. It also explores their application in studying drug-receptor interactions, screening chemical permeability, absorption, distribution, toxicity, as well as predicting environmental risks. Additionally, this review discusses the application of biomimetic magnetic nanoparticles, which leverage biological membranes and proteins for drug discovery, protein purification, and diagnostics.

Graphical Abstract

{"title":"Biomimetic separations in chemistry and life sciences","authors":"Fotios Tsopelas, Chrysanthos Stergiopoulos, Panagiotis Danias, Anna Tsantili-Kakoulidou","doi":"10.1007/s00604-025-06980-x","DOIUrl":"10.1007/s00604-025-06980-x","url":null,"abstract":"<div><p>Since Otto Schmitt introduced the term “biomimetics” in 1957, the imitation of biological systems to develop separation methods and simulate biological processes has seen continuous growth, particularly over the past five decades. The biomimetic approach relies on the use of specific ligands—biospecific, biomimetic, or synthetic—which target biomolecules, such as proteins, antibodies, nucleic acids, enzymes, drugs, pesticides, and other bioactive analytes. This review highlights advances in biomimetic separations, focusing on biomimetic liquid chromatography (including immobilized artificial membrane chromatography, cell membrane chromatography, biomimetic affinity chromatography, weak affinity chromatography, micellar liquid chromatography, immobilized liposome chromatography, and liposome electrokinetic capillary chromatography) for the complex separation and purification of biomolecules and other important chemical compounds. It also explores their application in studying drug-receptor interactions, screening chemical permeability, absorption, distribution, toxicity, as well as predicting environmental risks. Additionally, this review discusses the application of biomimetic magnetic nanoparticles, which leverage biological membranes and proteins for drug discovery, protein purification, and diagnostics.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-06980-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108329","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

NADPH-mediated seedless in situ formation of gold or gold-platinum nanoparticles for the enzymatic determination of atropine

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-04 DOI: 10.1007/s00604-025-06964-x

Mario Domínguez, Susana de Marcos, Javier Galbán

The without-seeds in situ formation of gold nanoparticles from NADPH and its application to the colorimetric determination of atropine (a tropane alkaloid) in cereals is reported. The method is based on a chemical hydrolysis, followed by an enzymatic oxidation by NADP catalyzed by tropinone reductase in the presence of Au(III) or Au(III)/Pt(II). During this reaction, the formed NADPH reduces the metal ion precursor to AuNPs (or AuPtNPs) and the absorption due to the plasmon band (550 nm or 575 nm) is measured. The method (AuPtNPs) allows the determination of the analyte in the concentration range 0.025 to 0.09 mM with an RSD of 3% (n = 5) and is applied to its determination in spiked buckwheat samples using the standard addition method, with 96.9 ± 2.0% recovery. It is also demonstrated that NAD(P)H and NADH have different kinetics for AuNP generation, which could be used to discriminate between these two cofactors.

Graphical Abstract

{"title":"NADPH-mediated seedless in situ formation of gold or gold-platinum nanoparticles for the enzymatic determination of atropine","authors":"Mario Domínguez, Susana de Marcos, Javier Galbán","doi":"10.1007/s00604-025-06964-x","DOIUrl":"10.1007/s00604-025-06964-x","url":null,"abstract":"<div><p>The without-seeds in situ formation of gold nanoparticles from NADPH and its application to the colorimetric determination of atropine (a tropane alkaloid) in cereals is reported. The method is based on a chemical hydrolysis, followed by an enzymatic oxidation by NADP catalyzed by tropinone reductase in the presence of Au(III) or Au(III)/Pt(II). During this reaction, the formed NADPH reduces the metal ion precursor to AuNPs (or AuPtNPs) and the absorption due to the plasmon band (550 nm or 575 nm) is measured. The method (AuPtNPs) allows the determination of the analyte in the concentration range 0.025 to 0.09 mM with an RSD of 3% (<i>n</i> = 5) and is applied to its determination in spiked buckwheat samples using the standard addition method, with 96.9 ± 2.0% recovery. It is also demonstrated that NAD(P)H and NADH have different kinetics for AuNP generation, which could be used to discriminate between these two cofactors.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 3","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-06964-x.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143108338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A turn-on fluorescent immunosensor for neurodegenerative disease related neurofilament light chain protein

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-02-04 DOI: 10.1007/s00604-025-06995-4

Qingting Song, Hailong Zhang, Jia Kong, Man Shing Wong, Hung-Wing Li

Neurodegenerative diseases (NDs) pose significant challenges to both longevity and quality of life, affecting millions worldwide and ranking as a major cause of global morbidity and disability. There is an urgent need for less invasive, cost-effective diagnostic methods that can reliably detect ND-related biomarkers in readily available biofluids such as serum. In response to this need, we have developed an ultra-sensitive assay that utilizes magnetic nanoparticles and a novel, tailor-made turn-on fluorescent probe named F-SPG. This innovative assay enables the detection of neurofilament light chain (NfL) at femtomolar concentrations, with a remarkable detection limit of 24 fM which is at least 18 times more sensitive than the conventional ELISA kits. Our assay’s design eliminates the need for traditional secondary antibody in immunoassay, thereby streamlining the diagnostic process. Recoveries exceeding 95% underscore the assay’s precision and reliability. It has proven effective in distinguishing Alzheimer’s Disease patients from healthy individuals by quantifying serum NfL levels, showcasing its potential as a cost-effective, ultra-sensitive tool for the early screening of neurodegenerative diseases.

Graphical abstract

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