Microchimica Acta最新文献

{"title":"Engineering fluorescent carbon dot sensor with rare earth europium for the detection of uranium (VI) ion in vivo","authors":"Xiayu Zhou,&nbsp;Yue Wang,&nbsp;Jiayi Song,&nbsp;Lihao Xiong,&nbsp;Xin Zhao,&nbsp;Sihan Chen,&nbsp;Weichao Zhao,&nbsp;Le Li,&nbsp;Deshuai Zhen","doi":"10.1007/s00604-025-07078-0","DOIUrl":"10.1007/s00604-025-07078-0","url":null,"abstract":"<div><p>Three types of carbon dots were synthesized using the same precursor (folic acid and europium nitrate) via different preparation methods (doping and direct coordination). A comprehensive comparison and analysis of the morphology, surface groups, and optical properties of the prepared carbon dots (CD), europium-doped carbon dots (CD-Eu), and europium-functionalized carbon dots (CD@Eu) were conducted. Moreover, due to the higher quantum yield, excellent stability, and outstanding selectivity for UO₂²⁺ exhibited by CD-Eu, we selected CD-Eu as the probe for subsequent applications. CD-Eu showed a sensitive response to UO₂²⁺ within the concentration range 25 ~ 200 nM, with a detection limit of 0.84 nM (0.42 μg·L⁻¹). Additionally, CD-Eu demonstrated excellent accuracy and recovery in spiked detection of real water samples. Furthermore, we discovered that this probe could detect UO₂²⁺ both <i>in</i> <i>vitro</i> and <i>in</i> <i>viv</i>o. This strategy provides a promising fluorescent sensor for the detection of UO₂²⁺ in water and biological samples, holding significant implications for addressing UO₂²⁺ contamination issues.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143583340","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A label-free colorimetric assay for sensitive screening of T4 polynucleotide kinase activity and inhibition based on enzyme-aided cyclic strand displacement amplification and PNA-gold nanoparticle platform","authors":"Xiaojun Yuan,&nbsp;Long Chen,&nbsp;Yang Zhao,&nbsp;Li Cheng,&nbsp;Chao Zhao","doi":"10.1007/s00604-025-07086-0","DOIUrl":"10.1007/s00604-025-07086-0","url":null,"abstract":"<div><p>The phosphorylation of nucleic acids mediated by 5′-polynucleotide kinase (PNK) exerts a crucial regulatory function in a wide range of significant cellular activities. Nevertheless, the current approaches for detecting PNK require expensive labeled probes and complex instrumentation, making it impossible to achieve real-time, on-site, and rapid analysis. Here, we take T4 PNK as a model and establish a novel colorimetric strategy for the detection of PNK activity and its inhibition by means of a coupled enzyme-assisted cyclic strand displacement amplification (SDA) and peptide nucleic acid (PNA)-gold nanoparticle (AuNP) based platform. The inspiration for this innovative strategy comes from the high stability, strong binding ability, and potent regulatory effect of PNA probes on AuNPs. Under the catalysis of PNK, the 5′-hydroxyl end of the hairpin-shaped DNA (hpDNA) is initially phosphorylated and subsequently digested by λ exonuclease (λ exo). This results in the release of a single-stranded DNA, which serves as a triggering factor to initiate the strand displacement reaction (SDR). The replaced PNA probe adheres to the surface of AuNPs, inducing their aggregation and causing a remarkable color change. Meanwhile, the double-stranded SDR product releases the SDR trigger with the aid of a nicking enzyme, triggering the next round of the SDR cycle and achieving highly efficient and controllable signal amplification. This assay is simple to operate and does not require bulky and expensive instruments or complex labeled probes. Compared with existing colorimetric methods, the detection sensitivity has been greatly improved, reaching 3.52 × 10⁻⁴ U/mL. Additionally, the method has demonstrated satisfactory results when applied to intricate biological matrices and the screening of T4 PNK inhibitors. Therefore, the proposed strategy holds significant potential for real-time analysis, high-throughput detection, and PNK-related drug screening.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07086-0.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of trypsin-protected CsPbCl3 fluorescent nanocrystals for hydroxyl radical sensing","authors":"Suresh Kumar Kailasa,&nbsp;Kartik Pankajbhai Makwana,&nbsp;Madhura Pradeep Deshpande,&nbsp;Yoojin Choi,&nbsp;Ruth Stephanie,&nbsp;Chan Yeong Park,&nbsp;Tae Jung Park","doi":"10.1007/s00604-025-07070-8","DOIUrl":"10.1007/s00604-025-07070-8","url":null,"abstract":"<div><p>Water-dispersible perovskite nanocrystals (PNCs) show promising applications in recognizing ionic and molecular species because of their excellent optical properties. However, lead halide PNCs have some limitations when they are used as probes for molecular species sensing in aqueous media. Here, we introduce trypsin (Try) as a bioligand for the synthesis of cesium lead chloride (CsPbCl₃) PNCs with high water stability. The as-fabricated Try-CsPbCl₃ PNCs show λ_Em/Ex at 433/370 nm with a quantum yield of 17.26%. The fluorescence emission spectral characteristics of Try-CsPbCl₃ PNCs demonstrated that water-stable Try-CsPbCl₃ PNCs acted as a promising fluorescent probe for the detection of hydroxyl radical (^•OH) via turn-off mechanism. The Try-CsPbCl₃ PNCs-based turn-off fluorescence approach displayed good selectivity for hydroxyl radical in water, showing a wider linear range (0.01–5 µM) with a remarkable detection limit of 3.10 nM for hydroxyl radical. The Try-CsPbCl₃ PNCs were demonstrated to be a facile probe for sensing ^•OH in water samples, which signifies that Try-CsPbCl₃ PNCs exhibited broad applications for hydroxyl radical sensing in real samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07070-8.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581260","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioaccessibility and cellular transport study of silver and titanium dioxide nanoparticles from exposed seaweed and mussels using Caco-2 cells","authors":"Juan José López-Mayán,&nbsp;Raquel Domínguez-González,&nbsp;María Carmen Barciela-Alonso,&nbsp;Elena Peña-Vázquez,&nbsp;Antonio Moreda-Piñeiro,&nbsp;Pablo Taboada-Antelo,&nbsp;Pilar Bermejo-Barrera","doi":"10.1007/s00604-025-07066-4","DOIUrl":"10.1007/s00604-025-07066-4","url":null,"abstract":"<div><p>Bioavailability studies on pollution pre-concentrator organisms such as algae and mussels are necessary to ensure food safety, particularly in the case of nanomaterials whose industrial applications have increased in recent years. Thus, the bioaccessibility and the bioavailability of total Ag and Ti and AgNPs and TiO₂NPs from raw and cooked seaweed (<i>Palmaria palmata</i> and <i>Ulva</i> sp.) and cooked mussels (<i>Mytilus edulis</i>) exposed to 1.0 mg L⁻¹ of 15 nm PVP-AgNPs, 1.0 mg L⁻¹ of 25 nm citrate-TiO₂NPs, or 1.0 mg L⁻¹ 5 nm citrate-TiO₂NPs, for 28 days, were evaluated. Samples were subjected to an in vitro process simulating human gastrointestinal digestion while cell transport from the intestinal lumen to the bloodstream was mimicked using Caco-2 cell cultures. Total Ag and Ti in the digest samples, the bioaccessible fractions, the apical, and basolateral fractions were determined by ICP-MS, while AgNPs and TiO₂NPs were determined by single-particle-ICP-MS (SP-ICP-MS). Finally, the presence of nanoparticles in the Caco-2 cells was verified by single-cell-ICP-MS (SC-ICP-MS). AgNP bioaccessibility ratios were between 22% and 97% for seaweed and 18% for mussels, while for TiO₂NPs were between 17% and 81% (seaweed) and 76% and 100% (mussels). Nanoparticle cellular transports were in all cases less than 1%. However, the mass percentages of Ag as NPs and Ti as NPs in the Caco-2 cells for raw and cooked seaweed were 9% and 7% and 20% and 6%, respectively. These results confirm a small transport of the nanoparticles through the Caco-2 cells under the proposed experimental conditions.\u0000</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07066-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143581152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Fluorescent aptasensor for detection of Salmonella typhimurium using boric acid-functionalized terbium metal–organic framework and magnetic nanoparticles","authors":"Dinesh Kumar,&nbsp;Harpreet Singh,&nbsp;Simran Makkar,&nbsp;Nitin Singhal,&nbsp;Akash Deep,&nbsp;Sanjeev Soni","doi":"10.1007/s00604-025-07073-5","DOIUrl":"10.1007/s00604-025-07073-5","url":null,"abstract":"<div><p>A fluorescent detection platform was designed using boric acid-functionalized terbium metal–organic framework (BA-Tb-MOF) and carboxyl-modified magnetic nanoparticles (MNPs) to identify <i>Salmonella typhimurium </i>(<i>S. typhimurium</i>) bacteria. Firstly, carboxyl-modified Fe₃O₄MNPs were coated with specific aptamer (Apt-MNPs) as the capture probe for <i>S. typhimurium</i>. Then, the Apt-MNPs were added to the bacterial suspension to facilitate the targeted binding. Subsequently, the fluorescent probe (BA-Tb-MOF) was introduced into this solution. The BA-Tb-MOF was strongly attached to the bacterial surface through interactions between BA and glycolipids on the bacterial cell walls, forming a stable complex. As the bacterial concentration increased, the fluorescence intensity of the solution progressively decreased due to the binding and removal of bacteria-Apt-MNPs/BA-Tb-MOF complexes through magnetic separation. Under optimum conditions, the concentration of <i>S. typhimurium</i> and the fluorescence intensity showed an inverse linear relationship within the range of 10¹–10⁹ CFU/mL, and the detection limit was 4 CFU/mL. The developed sensor showed high specificity against several other pathogenic bacteria such as <i>E. coli</i>, <i>S. aureus</i>, and <i>P. aeruginosa</i>. The developed fluorescence platform also successfully detected the <i>S. typhimurium</i> in drinking water and egg samples with satisfactory recoveries (83–98%). This strategy can be investigated further for the detection of <i>S. typhimurium</i> and other pathogens in food and clinical samples.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143570891","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasensitive detection of doxycycline enabled by oxygen vacancy modulated TiO2 nanotubes","authors":"Juan Gao,&nbsp;Sen Yang,&nbsp;Chen Xu,&nbsp;Zerui Dong,&nbsp;SiZhu Chen,&nbsp;Lingcheng Zheng,&nbsp;Leilei Lan,&nbsp;Gang He","doi":"10.1007/s00604-025-07072-6","DOIUrl":"10.1007/s00604-025-07072-6","url":null,"abstract":"<div><p>TiO₂ nanotubes rich in oxygen vacancies (Ov), which were successfully fabricated on Ti foils, were used as the working electrode of a photoelectrochemical (PEC) sensor. The TiO₂ nanotube electrode optimized with abundant Ov demonstrated a remarkable photocurrent density of 1.03 mA/cm², which is approximately 2.9 times higher than that of the TiO₂ nanotube electrode. When applied to the detection of DOC, this electrode exhibited a wide linear detection range spanning from 0.1 to 100 μM and achieved an exceptionally low detection limit of 0.043 μM with a signal-to-noise ratio of 3. Furthermore, comparative experiments indicated that the Ov-enriched TiO₂ nanotube electrode exhibited excellent anti-interference capabilities and long-term stability, ensuring the accuracy and reliability of the detection outcomes. The superior detection performance is primarily attributed to two aspects: on one hand, Ov act as electron traps, facilitating the capture and transfer of photogenerated electrons, effectively prolonging the lifetime of these carriers; on the other hand, Ov also serves as active sites, enhancing the adsorption of DOC molecules and reaction kinetics, further amplifying the detection signal. This work offers a theoretical and experimental groundwork for the rapid monitoring of residual antibiotics.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div><div><p>Graphical Abstract</p></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound meets nanomedicine: towards disease treatment and medical imaging","authors":"Xiaochun Li,&nbsp;Yanting Liu,&nbsp;Xuewan Wu,&nbsp;Rui Huang,&nbsp;Shaoqi Chen,&nbsp;Kaisong Yuan","doi":"10.1007/s00604-025-07042-y","DOIUrl":"10.1007/s00604-025-07042-y","url":null,"abstract":"<div><p>As a kind of mechanical wave, ultrasound has been widely employed in the biomedical field due to its superiors of deep tissue penetration, non-destructiveness and non-toxicity. In this review, we highlight current progress and prospects in using ultrasound as a powerful tool for disease treatment and medical imaging, including (1) ultrasound as energy input for driving nano/micromotor in drug delivery, this part first introduces the synthesis and motion behavior of nano/micromotors, then reviews the small molecular and macromolecular compounds that the nano/micromotors are delivering; (2) sonosensitive nanomaterials for disease therapy, the sonodynamic, sonopiezoelectric, sonothermal, and sonomechanical therapy will all be covered; (3) ultrasound as a non-invasive technique for nano/micromotor tracking or medical imaging; (4) the sonoporation of nano/microbubble. Future challenges in using ultrasound for disease treatment or medical imaging will also be described in the conclusion part.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143571132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of a PCR fluorescence sensor utilizing an upconversion FRET system for rapid and ultra-sensitive determination of Escherichia coli","authors":"Lu Han,&nbsp;Yaqi Song,&nbsp;Min Chen,&nbsp;Leiqing Pan,&nbsp;Kang Tu,&nbsp;Jing Su","doi":"10.1007/s00604-025-07077-1","DOIUrl":"10.1007/s00604-025-07077-1","url":null,"abstract":"<div><p>An upconversion-gold nanoparticle detection system that integrates PCR amplification and fluorescence resonance energy transfer was constructed to enable swift and highly sensitive identification of <i>Escherichia coli</i>. The forward primer used in the PCR amplification is modified with sulfhydryl groups, enabling its connection to gold nanoparticles via Au–S bonds. The complementary strand of the forward primer, which is attached to the upconversion nanomaterials, can hybridize with the free forward primer through base complementary pairing. This interaction induces fluorescence resonance energy transfer, resulting in fluorescence quenching. The concentration of the target bacteria influences the amount of free primer in the system after PCR amplification, which subsequently alters the intensity of the upconversion fluorescence. The fluorescent PCR sensor developed based on the aforementioned principles demonstrated a detection limit of 14 CFU/mL for <i>E. coli</i>, with a quantitative detection range of 18–1.8 × 10⁷ CFU/mL. In comparison to the qPCR method, the number of PCR cycles required for the constructed biosensor can be reduced to approximately 22 to achieve the same detection limit, effectively decreasing the detection time by about 24 min. The spiked recoveries in chicken were 91.8–106.0% with the relative standard deviations less than 10%, indicating that the constructed method exhibits good applicability.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface-enhanced Raman spectroscopy in pharmaceutical analysis: from component determination to mechanism research","authors":"Ling Xia,&nbsp;Xiaoyu Qi,&nbsp;Huimin Yu,&nbsp;Yang Li","doi":"10.1007/s00604-025-07029-9","DOIUrl":"10.1007/s00604-025-07029-9","url":null,"abstract":"<div><p>Surface-enhanced Raman spectroscopy (SERS) technology, possessing extraordinary sensitivity and an outstanding ability to furnish molecular-level information, has emerged as a revolutionary technique within the domain of analytical chemistry. This review comprehensively and in-depthly investigates the application of SERS in the field of pharmaceutical analysis, encompassing detailed analyses in diverse aspects such as ingredient identification, quality control, mechanism of action, and efficacy evaluation. Firstly, we introduce the key aspects of SERS technology, including its working principle, substrate preparation methods, and crucial factors in the experimental design process. Subsequently, the specific application of SERS in drug ingredient analysis is elaborated in detail, emphasizing its capability to rapidly identify active ingredients in complex mixtures, particularly the significant advantages it exhibits when tracking low-concentration bioactive molecules. At the quality control level, the application of SERS can precisely identify illegal adulteration in regular drugs, effectively guaranteeing the quality and consistency of drugs. Additionally, SERS also demonstrates great potential in analyzing the mechanism of action of natural drugs. It can clearly disclose the interaction between drugs and organisms at the molecular level and evaluate the effect of drugs. Although SERS technology has manifested extremely significant application prospects in the field of drug research, its widespread implementation still encounters some challenges, including the standardization of substrates, the reproducibility of signals, and the complexity of data processing. The future development direction will concentrate on the innovative design of substrates, the automation of the analysis process, and the organic integration with other bioanalytical technologies to enhance its accuracy and practicability in pharmaceutical analysis. Through continuous technological innovation and interdisciplinary cooperation, SERS technology is anticipated to play a more crucial role in the research and development of drugs, further promoting the continuous growth and innovation of the drug industry. This article aims to provide researchers in related fields with a comprehensive perspective on the current application status and future development trend of SERS technology, and also offer scientific basis and technical support for drug quality control and new drug development.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Air plasma assisted directional electrodeposition of Ag nanoparticles on carbon cloth for electrochemical detection of rutin","authors":"Jing Ren,&nbsp;Yutong An,&nbsp;Shiqi Yin,&nbsp;Jun Wang,&nbsp;Qingxian Yu,&nbsp;Chunfang Li,&nbsp;Tianrong Zhan","doi":"10.1007/s00604-025-07068-2","DOIUrl":"10.1007/s00604-025-07068-2","url":null,"abstract":"<div><p>The air plasma-induced defects and O-related groups are employed as anchoring sites for the directional electrochemical deposition of Ag nanoparticles (AgNPs) with even distribution and a narrow size range on carbon cloth (CC). The strategy of integrating the air plasma and AgNPs provides plentiful adsorption and catalytic sites, a large electroactive area, superhydrophilic interface, and a smooth charge and mass-transfer pathway. As a consequence, the fabricated self-supporting electrode of AgNPs@CC − P displays an excellent sensing performance toward rutin with a large linear concentration window (0.05 ~ 30 µM), a small LOD (4.2 nM, S/N = 3) and LOQ (32 nM, S/N = 10), as well as good stability and reproducibility. The fabricated sensor is successfully applied to the practical detection of rutin in tablets and beverages with good accuracy. The self-supporting sensor also shows potential for fabricating flexible and soft sensing devices for real-time monitoring. </p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143564338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}