Microchimica Acta最新文献_第5页

Bio-based plasticizer Babassu oil for custom-made conductive additive-manufacturing filaments: towards 3D-printed electrodes applied to cocaine detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-31 DOI: 10.1007/s00604-024-06910-3

João Pedro C. Silva, Raquel G. Rocha, Gilvana P. Siqueira, Caio F. Nascimento, Mario H. P. Santana, Edson Nossol, Eduardo M. Richter, Iranaldo S. da Silva, Rodrigo A. A. Muñoz

Babassu (Atallea sp.), a native palm tree from South America’s Amazon produces bio-oil and biochar with significant potential for industrial applications. Babassu oil as a bio-based plasticizer is reported here for the first time to replace petrochemical alternatives in the production of conductive filaments for additive manufacturing purposes. The newly developed filament, consisting of 30 wt.% carbon black (CB), 60 wt.% polylactic acid (PLA), and 10 wt.% Babassu oil, demonstrates enhanced thermal stability, improved printability, and superior electrochemical performance. 3D-printed electrodes made from this Babassu-CB/PLA filament outperform those made from commercial CB/PLA filaments, exhibiting better voltammetric responses (e.g., lower peak-to-peak separation and higher current intensity) for inner-sphere redox probes. As a proof-of-concept, these bio-based electrodes were successfully used to detect cocaine in real-world street samples, with a limit of detection (LOD) of 1.2 µmol L⁻¹. The Babassu-CB/PLA electrodes provided reliable cocaine quantification without interference from common adulterants such as caffeine, paracetamol, and phenacetin and showed no matrix effects, as confirmed by recovery values ranging from 88 to 102%. Notably, the use of Babassu oil eliminates the need for laborious post-treatment protocols to achieve optimal electrochemical performance. In this study, only mechanical polishing on the electrode surface is required. This study highlights the potential of Babassu oil as a sustainable alternative in the fabrication of conductive filaments for electrochemical applications, advancing the field of environmentally friendly and efficient 3D-printed sensors.

Graphical Abstract

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引用次数: 0

Cutting-edge 3D printing in immunosensor design for early cancer detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06880-6

Sachin Kothawade, Vijaya Padwal

Cancer is a major cause of death globally, and early detection is a key to improving outcomes. Traditional diagnostic methods have limitations such as being invasive and lacking sensitivity. Immunosensors, which detect cancer biomarkers using antibodies, offer a solution with high sensitivity and selectivity. When combined with 3D printing, these immunosensors can be customized to detect specific cancer markers, creating rapid, cost-effective, and scalable diagnostic tools. The article reviews the principles behind immunosensors, different 3D fabrication methods such as Fused Deposition Modeling (FDM) and Stereolithography (SLA), and discusses how functionalization strategies, such as surface modifications, can enhance the sensitivity of these devices. The integration of 3D printing allows for the creation of complex sensor structures, offering advantages such as customization, rapid prototyping, and multi-material printing. These advancements make immunosensors arrays highly promising for early cancer detection, tumor profiling, and personalized medicine. The article also explores challenges like scalability, material biocompatibility, and the need for clinical validation. Future perspectives suggest the potential of integrating nanomaterials, multiplexed detection, and wearable technology to further improve the performance and accessibility of these diagnostic tools.

Graphical Abstract

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引用次数: 0

Covalent organic frameworks hybridized polymeric high internal phase emulsions with amphiphilicity for extraction of trace bisamide insecticides in food samples

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06890-4

Lin Su, Zhuqiang Wu, Guihua Ruan, Yipeng Huang

Polymeric high internal phase emulsions decorated with covalent organic frameworks (polyHIPEs-COFs) were synthesized and used as the sorbent for cyantraniliprole and chlorantraniliprole. Pickering high internal phase emulsions stabilized by covalent organic frameworks solid particles and liquid surfactants (Span80 and polyvinylpyrrolidone) endow the composites with open-cell structures and superwettability. The amphiphilicity and open-cell structures enable rapid adsorption and desorption for cyantraniliprole and chlorantraniliprole, and the solid-phase extraction process can be completed in 5 min. The adsorption efficiencies of polyHIPEs-COFs for cyantraniliprole and chlorantraniliprole are above 85.19%, but lower than 10% for fenvalerate, anti-aphid, and chlorpyrifos, demonstrating the good adsorption selectivity for cyantraniliprole and chlorantraniliprole. The adsorption efficiencies of cyantraniliprole and chlorantraniliprole using a same polyHIPEs-COFs and five different batches of polyHIPEs-COFs range from 94.25 to 100.00%, revealing the good reproducibility of the sorbent. In addition, the polyHIPEs-COF-based solid-phase extraction combined with high-performance liquid chromatography-ultraviolet detector (HPLC–UV) was developed for determination of bisamide insecticides in vegetable (eggplants, tomatoes, and peppers) samples. Results showed that the method was feasible to determine the cyantraniliprole and chlorantraniliprole in real vegetable samples with a linear range of 0.012–1.2 μg/kg and limits of detection of 0.0075–0.0090 μg/kg. The recoveries of cyantraniliprole and chlorantraniliprole spiked in vegetable samples ranged from 85.00 to 100.00% with relative standard deviations less than 3.52%. The study indicates the feasibility of amphiphilic polyHIPEs-COFs in extraction and enrichment of bisamide insecticides from vegetable samples for HPLC–UV analysis.

Graphical Abstract

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引用次数: 0

Robust and efficient separation of white blood cells from blood using a microfluidic chip with a pair of linearly tapered crossflow filter arrays

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06913-0

Yuanding Huang, Ping Chen, Meng Niu, Weng Kung Peng

Clinical and immunological assays of white blood cells (WBCs) in human peripheral blood are of significance for disease diagnosis and immunological studies. However, separating WBCs from blood with high recovery and high purity remains challenging. In this study, by incorporating a pair of linearly tapered filter arrays, a crossflow filtration-based microfluidic chip was designed and fabricated for separation of WBCs from blood. The implementation of the linearly tapered filter arrays not only ensures a minimal and consistent flow through each sieve, but also achieves a high filtration ratio (~ 19). The validity and robustness of this straightforward design were substantiated through theoretical analysis, simulations, and model microparticle tests. The microfluidic chip achieved an almost perfect (> 99.2%) recovery and a ~ 20-fold enrichment of the targeted 8 μm particles (as surrogates for WBCs) from undesired 2 μm particles (as substitutes for red blood cells, RBCs) at flow rates ranging from 50 to 200 μL/min, irrespective of the filter array length and particle concentration. When applied to a twenty times diluted blood sample, the chip achieved a 96.6% recovery and 19.7-fold enrichment of WBCs, as well as a 95.0% removal of RBCs, at the optimal flow rate of 100 μL/min. With its simple design, cost-effectiveness, high recovery, substantial enrichment ratio, and considerable throughput, this chip offers an alternative solution that is potentially applicable to scenarios involving the separation of WBCs as well as other particles/cells.

Graphical abstract

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引用次数: 0

Amino-functionalized HPU-23@Ru@Tb as light-driven oxidase-like nanozyme for colorimetric recognition of Hg2+ and ratiometric fluorescence sensing of ClO− and PO43−

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06874-4

Xiang Liu, Huijun Li, Junjun Sun, Jianchao Shi, Zhouqing Xu, Yan Wang

A HPU-23@Ru@Tb-NH₂ sensor array with light-driven oxidase-mimicking activity and triple-emission fluorescence was developed. It was composed of a Tb³⁺-functionalized metal organic framework and Ru(bpy)₃²⁺ and applied to the simultaneous detection of Hg²⁺, ClO⁻, and PO₄³⁻ via differently responsive channels. HPU-23@Ru@Tb-NH₂ had a photoresponsive colorimetric response toward Hg²⁺ with a LOD as low as 4.18 nM. In addition, the three emissions of the HPU-23@Ru@Tb-NH₂ sensor array were influenced by ClO⁻ and PO₄³⁻ to varying degrees, causing remarkably distinguishable responses for the fluorescence channels to discriminate ClO⁻ and PO₄³⁻ from each other. The detection limits of ClO⁻ and PO₄³⁻ were 12.26 µM and 0.197 nM, respectively. Therefore, this work demonstrates the feasibility of multi-emission and multi-mode sensing platform, which is able to combine the advantages of different strategies for solving the problems of various toxic substances coexisting in the environment while meeting the needs of accurate and precise results and no side interferences.

Graphical Abstract

{"title":"Amino-functionalized HPU-23@Ru@Tb as light-driven oxidase-like nanozyme for colorimetric recognition of Hg2+ and ratiometric fluorescence sensing of ClO− and PO43−","authors":"Xiang Liu, Huijun Li, Junjun Sun, Jianchao Shi, Zhouqing Xu, Yan Wang","doi":"10.1007/s00604-024-06874-4","DOIUrl":"10.1007/s00604-024-06874-4","url":null,"abstract":"<div><p> A HPU-23@Ru@Tb-NH<sub>2</sub> sensor array with light-driven oxidase-mimicking activity and triple-emission fluorescence was developed. It was composed of a Tb<sup>3+</sup>-functionalized metal organic framework and Ru(bpy)<sub>3</sub><sup>2+</sup> and applied to the simultaneous detection of Hg<sup>2+</sup>, ClO<sup>−</sup>, and PO<sub>4</sub><sup>3−</sup> via differently responsive channels. HPU-23@Ru@Tb-NH<sub>2</sub> had a photoresponsive colorimetric response toward Hg<sup>2+</sup> with a LOD as low as 4.18 nM. In addition, the three emissions of the HPU-23@Ru@Tb-NH<sub>2</sub> sensor array were influenced by ClO<sup>−</sup> and PO<sub>4</sub><sup>3−</sup> to varying degrees, causing remarkably distinguishable responses for the fluorescence channels to discriminate ClO<sup>−</sup> and PO<sub>4</sub><sup>3−</sup> from each other. The detection limits of ClO<sup>−</sup> and PO<sub>4</sub><sup>3−</sup> were 12.26 µM and 0.197 nM, respectively. Therefore, this work demonstrates the feasibility of multi-emission and multi-mode sensing platform, which is able to combine the advantages of different strategies for solving the problems of various toxic substances coexisting in the environment while meeting the needs of accurate and precise results and no side interferences.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive bromate sensor based on a gold nanoparticle-poly(diallyldimethylammonium chloride)–reduced graphene oxide nanocomposite modified glassy carbon electrode

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06871-7

Ai-ping Zhang, Yi-xuan Fan, Ning Wang, Hao Yu

A nanocomposite consisting of gold nanoparticles (AuNPs), poly(diallyldimethylammonium chloride) (PDDA), and reduced graphene oxide (rGO) was fabricated by a two-step chemical reduction method. Firstly, a PDDA-rGO composite was prepared by using hydrazine hydrate as a reducing agent. Subsequently, the AuNP-PDDA-rGO composite was prepared in ethylene glycol with PDDA-rGO and HAuCl₄ as raw materials using sodium citrate as a reduction agent. The resulting composite was characterized using X-ray powder diffraction (XRD), Raman spectroscopy, transmission electron microscopy (TEM), and electrochemical methods. This composite was then modified on a glassy carbon electrode (GCE) by the dropping method. The electrochemical behavior of bromate on this modified electrode was investigated. The results showed that PDDA-rGO can be used as a good carrier to obtain AuNPs with small particle sizes and good dispersion. The AuNPs and PDDA-rGO in composite enhanced the electrochemical activity of the electrode. Under the synergistic action of each component, the resulting electrode exhibited high activity for the electrochemical reduction of bromate. Based on this, an amperometric bromate sensor was fabricated in N₂-saturated 0.10 mol/L HCl with attractive features including a wide linear range of 1.0 × 10⁻⁷–1.7 × 10⁻³ mol/L, a low detection limit (3s_b) of 3.2 × 10⁻⁸ mol/L, and a high sensitivity of 2317 µA/mM/cm². The sensor was successively used for the determination of bromate in drinking water.

Graphical Abstract

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引用次数: 0

An electrochemiluminescence biosensor based on silver-cysteine nanorod as an emitter and AgNP-decorated FeMoOν as a signal amplifier for sensitive detection of heart-type fatty acid binding protein

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-30 DOI: 10.1007/s00604-024-06923-y

Lingling Zheng, Jing Li, Shenglan Hu, Lixin Xu, Yusheng Wu, Biyang Deng

An electrochemiluminescence (ECL) immunosensor was developed for the highly sensitive and specific detection of heart-type fatty acid binding protein (H-FABP) and the rapid diagnosis of acute myocardial infarction (AMI). H-FABP is a biomarker that is highly specific to cardiac tissue and is associated with a range of cardiac diseases. Following myocardial injury, the rate of increase in H-FABP levels is greater than that observed for myoglobin and troponin. Therefore, the measurement of H-FABP is crucial for the early exclusion of AMI. Silver-cysteine nanorod (AgCysNR), which served as the ECL emitter, was produced with a one-step, green, simple, template-free aqueous phase method. The surfaces of AgCysNR displayed many amino and carboxyl groups that were connected to a large number of a secondary H-FABP-specific antibody. Ferrum-doped molybdenum oxide (FeMoO_ν), with a large specific surface area, was richly decorated with silver nanoparticle (AgNP), which increased the interfacial electron transfer rate of FeMoO_ν. The AgNP was used as a co-reaction accelerator to promote persulfate to produce more sulfate anion radical and then enhance the ECL intensity of AgCysNR. The linear range of the ECL immunosensor was 10 fg/mL to 100 ng/mL, and the detection limit was 2.3 fg/mL (signal/noise = 3). The sensor was determined to be stable, repeatable, and reproducible, and the method achieved recoveries of 101.0 to 102.6% with relative standard deviations of 1.4 to 2.0%. This immunosensor represents a promising tool for the early diagnosis of AMI.

Graphical abstract

{"title":"An electrochemiluminescence biosensor based on silver-cysteine nanorod as an emitter and AgNP-decorated FeMoOν as a signal amplifier for sensitive detection of heart-type fatty acid binding protein","authors":"Lingling Zheng, Jing Li, Shenglan Hu, Lixin Xu, Yusheng Wu, Biyang Deng","doi":"10.1007/s00604-024-06923-y","DOIUrl":"10.1007/s00604-024-06923-y","url":null,"abstract":"<div><p>An electrochemiluminescence (ECL) immunosensor was developed for the highly sensitive and specific detection of heart-type fatty acid binding protein (H-FABP) and the rapid diagnosis of acute myocardial infarction (AMI). H-FABP is a biomarker that is highly specific to cardiac tissue and is associated with a range of cardiac diseases. Following myocardial injury, the rate of increase in H-FABP levels is greater than that observed for myoglobin and troponin. Therefore, the measurement of H-FABP is crucial for the early exclusion of AMI. Silver-cysteine nanorod (AgCysNR), which served as the ECL emitter, was produced with a one-step, green, simple, template-free aqueous phase method. The surfaces of AgCysNR displayed many amino and carboxyl groups that were connected to a large number of a secondary H-FABP-specific antibody. Ferrum-doped molybdenum oxide (FeMoO<sub>ν</sub>), with a large specific surface area, was richly decorated with silver nanoparticle (AgNP), which increased the interfacial electron transfer rate of FeMoO<sub>ν</sub>. The AgNP was used as a co-reaction accelerator to promote persulfate to produce more sulfate anion radical and then enhance the ECL intensity of AgCysNR. The linear range of the ECL immunosensor was 10 fg/mL to 100 ng/mL, and the detection limit was 2.3 fg/mL (signal/noise = 3). The sensor was determined to be stable, repeatable, and reproducible, and the method achieved recoveries of 101.0 to 102.6% with relative standard deviations of 1.4 to 2.0%. This immunosensor represents a promising tool for the early diagnosis of AMI.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142906014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Rapid evaluation of hepatocellular carcinoma by detecting plasma exosomes with time-resolved fluorescence immunochromatographic test strips

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06903-2

Jiaming Li, Jianfen Su, Minghui Li, Yaofen Wu, Huiqiang Chen, Xihua Fu, Hongliang Yao, Jinping Chen, Yuntao Liu, Jie Zan

Time-resolved fluorescence immunochromatographic test strips (TRFIS) was developed for the rapid detection of hepatocellular carcinoma (HCC)-specific plasma exosomes (hExos) by targeting the hExo-surface membrane protein glypican-3 (GPC3). The GPC3-TRFIS could directly detect plasma exosomes without the isolation and purification process, and the whole immunoassay could be completed within 15 min. The visual detection limit of GPC3-TRFIS was 3.44 × 10^9 particles/mL, with a minimum detection limit of 1.8 × 10^9 particles/mL. For analysis of the clinical HCC samples, GPC3-TRFIS shows high specificity for detection of hExo, and was nearly unreactive for healthy donors’ samples. GPC3-TRFIS was able to efficiently distinguish HCC patients (19 cases) from healthy donors (19 cases). Overall, the developed TRFIS offers the benefits of high sensitivity, simple operation, and no need of large precision instruments and professional technical personnel for rapid detection of plasma hExos, and supplies a novel approach for early screening of HCC.

Graphical Abstract

{"title":"Rapid evaluation of hepatocellular carcinoma by detecting plasma exosomes with time-resolved fluorescence immunochromatographic test strips","authors":"Jiaming Li, Jianfen Su, Minghui Li, Yaofen Wu, Huiqiang Chen, Xihua Fu, Hongliang Yao, Jinping Chen, Yuntao Liu, Jie Zan","doi":"10.1007/s00604-024-06903-2","DOIUrl":"10.1007/s00604-024-06903-2","url":null,"abstract":"<div><p>Time-resolved fluorescence immunochromatographic test strips (TRFIS) was developed for the rapid detection of hepatocellular carcinoma (HCC)-specific plasma exosomes (hExos) by targeting the hExo-surface membrane protein glypican-3 (GPC3). The GPC3-TRFIS could directly detect plasma exosomes without the isolation and purification process, and the whole immunoassay could be completed within 15 min. The visual detection limit of GPC3-TRFIS was 3.44 × 10^9 particles/mL, with a minimum detection limit of 1.8 × 10^9 particles/mL. For analysis of the clinical HCC samples, GPC3-TRFIS shows high specificity for detection of hExo, and was nearly unreactive for healthy donors’ samples. GPC3-TRFIS was able to efficiently distinguish HCC patients (19 cases) from healthy donors (19 cases). Overall, the developed TRFIS offers the benefits of high sensitivity, simple operation, and no need of large precision instruments and professional technical personnel for rapid detection of plasma hExos, and supplies a novel approach for early screening of HCC.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrafine metal–organic framework @ graphitic carbon with MoS2-CNTs nanocomposites as carbon-based electrochemical sensor for ultrasensitive detection of catechin in beverages

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06901-4

Yilin Wang, Zixia Zhao, Yuefan Wang, Zhifang Liu, Leyao Chen, Jin Qi, Yixi Xie, Pengcheng Zhao, Junjie Fei

GO/Co-MOF/PPy-350 (GPC-350) was synthesized by in situ growth of ultrafine Co-MOF on graphene oxide (GO), followed by encapsulation with polypyrrole (PPy) and calcination at 350.0℃. Meanwhile, MoS₂-MWCNTs (MoS₂-CNTs) were produced via the in situ synthesis of MoS₂ within multi-walled carbon nanotubes (MWCNTs). The electrochemically superior GPC-350/MoS₂-CNTs nanocomposite was then achieved by combining GPC-350 with MoS₂-CNTs. The polypyrrole encapsulation serves to protect the ultrafine Co-MOF, preventing its degradation during the calcination process. The linear detection range of the GPC-350/MoS₂-CNTs/GCE sensor for the determination of catechin (CA) in phosphate buffered saline (PBS) was from 5.0 to 1800.0 nM with a limit of detection of 1.78 nM. In addition, the materials were characterized using SEM, EDX, TEM, XRD, EIS, XPS, FTIR, and Raman. These results indicate that the synthesis of GPC-350/MoS₂-CNTs nanocomposites is successful and CA in beverages samples can be effectively detected using electrochemical sensors. Additionally, the reaction mechanism of CA was explored through cyclic voltammetry. The application of GPC-350/MoS₂-CNTs nanocomposites in sensor technology offers innovative approaches for the ultrasensitive detection of flavonoids.

Graphical abstract

{"title":"Ultrafine metal–organic framework @ graphitic carbon with MoS2-CNTs nanocomposites as carbon-based electrochemical sensor for ultrasensitive detection of catechin in beverages","authors":"Yilin Wang, Zixia Zhao, Yuefan Wang, Zhifang Liu, Leyao Chen, Jin Qi, Yixi Xie, Pengcheng Zhao, Junjie Fei","doi":"10.1007/s00604-024-06901-4","DOIUrl":"10.1007/s00604-024-06901-4","url":null,"abstract":"<div><p>GO/Co-MOF/PPy-350 (GPC-350) was synthesized by in situ growth of ultrafine Co-MOF on graphene oxide (GO), followed by encapsulation with polypyrrole (PPy) and calcination at 350.0℃. Meanwhile, MoS<sub>2</sub>-MWCNTs (MoS<sub>2</sub>-CNTs) were produced via the in situ synthesis of MoS<sub>2</sub> within multi-walled carbon nanotubes (MWCNTs). The electrochemically superior GPC-350/MoS<sub>2</sub>-CNTs nanocomposite was then achieved by combining GPC-350 with MoS<sub>2</sub>-CNTs. The polypyrrole encapsulation serves to protect the ultrafine Co-MOF, preventing its degradation during the calcination process. The linear detection range of the GPC-350/MoS<sub>2</sub>-CNTs/GCE sensor for the determination of catechin (CA) in phosphate buffered saline (PBS) was from 5.0 to 1800.0 nM with a limit of detection of 1.78 nM. In addition, the materials were characterized using SEM, EDX, TEM, XRD, EIS, XPS, FTIR, and Raman. These results indicate that the synthesis of GPC-350/MoS<sub>2</sub>-CNTs nanocomposites is successful and CA in beverages samples can be effectively detected using electrochemical sensors. Additionally, the reaction mechanism of CA was explored through cyclic voltammetry. The application of GPC-350/MoS<sub>2</sub>-CNTs nanocomposites in sensor technology offers innovative approaches for the ultrasensitive detection of flavonoids.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle–based CdTe quantum dots for sensitive zearalenone determination

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2024-12-28 DOI: 10.1007/s00604-024-06914-z

Xi Ma, Minyi Hui, Jiayu Yuan, Zhouping Wang, Xiaoyuan Ma

A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto Fe₃O₄@SiO₂ and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals. The two parts were merged based on the principle of base complementary pairing, resulting in an assembled structure exhibiting a diminished fluorescence signal due to the Förster resonance energy transfer (FRET) effect. Due to the higher affinity of the aptamer towards the target, the presence of ZEN resulted in the detachment of COF-Au, leading to an increase in supernatant concentration of COF-Au proportional to ZEN concentration. Consequently, this enhanced the catalytic ability and amplified the colorimetric signal. The fluorescence of precipitation increased simultaneously with the reduction of FRET, enabling linear detection of colorimetry in the range 0.5 ~ 10,000 μg·kg⁻¹ and fluorescence in the range 0.1 ~ 10,000 μg·kg⁻¹, with respective detection limits of 0.36 μg·kg⁻¹ and 0.09 μg·kg⁻¹. The spike recovery in wheat flour and corn ranged from 93.4 to 122.0%. This technology was simple to operate and had low cost and good application prospects.

Graphical Abstract

{"title":"Construction of colorimetric-fluorescent dual-signal aptamer-based assay using COF-Au nanozyme and magnetic nanoparticle–based CdTe quantum dots for sensitive zearalenone determination","authors":"Xi Ma, Minyi Hui, Jiayu Yuan, Zhouping Wang, Xiaoyuan Ma","doi":"10.1007/s00604-024-06914-z","DOIUrl":"10.1007/s00604-024-06914-z","url":null,"abstract":"<div><p>A dual-signal aptamer-based assay utilizing colorimetric and fluorescence techniques was developed for the determination of zearalenone (ZEN). The CdTe quantum dots, serving as the fluorescent signal source, were surface-modified onto Fe<sub>3</sub>O<sub>4</sub>@SiO<sub>2</sub> and subsequently functionalized with the aptamer. The COF-Au was modified with complementary chain, which possessed peroxide (POD)-like enzyme properties, and could catalyze the peroxidation of 3,3′,5,5′-tetramethylbenzidine (TMB) to ox TMB, resulting in the generation of colorimetric signals. The two parts were merged based on the principle of base complementary pairing, resulting in an assembled structure exhibiting a diminished fluorescence signal due to the Förster resonance energy transfer (FRET) effect. Due to the higher affinity of the aptamer towards the target, the presence of ZEN resulted in the detachment of COF-Au, leading to an increase in supernatant concentration of COF-Au proportional to ZEN concentration. Consequently, this enhanced the catalytic ability and amplified the colorimetric signal. The fluorescence of precipitation increased simultaneously with the reduction of FRET, enabling linear detection of colorimetry in the range 0.5 ~ 10,000 μg·kg<sup>−1</sup> and fluorescence in the range 0.1 ~ 10,000 μg·kg<sup>−1</sup>, with respective detection limits of 0.36 μg·kg<sup>−1</sup> and 0.09 μg·kg<sup>−1</sup>. The spike recovery in wheat flour and corn ranged from 93.4 to 122.0%. This technology was simple to operate and had low cost and good application prospects.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142890469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0