A lanthanide ion–based metal–organic framework (Eu-TATAB nanorods) was designed and synthesized as an effective tri-mode nanoprobe for sensitive and portable detection of ethanol content in a water–ethanol mixture. The assay was based on the responsive properties of Eu-TATAB nanorods to ethanol stimulus and their adaptive encapsulation capability towards optically active lanthanides. With the addition of ethanol to the Eu-TATAB nanorods, the structure was destroyed, resulting in a decrease in luminescence, electrochemiluminescence, and ultraviolet–visible spectrophotometric signals by perturbing energy transfer in the Eu-TATAB nanorods. According to the degree of the decrease in three luminescence intensities, the ethanol content could be quantitatively detected. Based on this, the rapid detection of alcohol content in different types of spirits and alcoholic beverages was further realized. This method is simple, convenient, and sensitive, and has good application prospects and development potential.
{"title":"Lanthanide ion–based metal–organic framework as an effective tri-mode nanoprobe for alcoholic content detection","authors":"Ning Xu, Xin Li, Chunyuan Tian, Zhiyang Zhang, Lingxin Chen, Feng Luan, Xuming Zhuang","doi":"10.1007/s00604-024-06915-y","DOIUrl":"10.1007/s00604-024-06915-y","url":null,"abstract":"<div><p> A lanthanide ion–based metal–organic framework (Eu-TATAB nanorods) was designed and synthesized as an effective tri-mode nanoprobe for sensitive and portable detection of ethanol content in a water–ethanol mixture. The assay was based on the responsive properties of Eu-TATAB nanorods to ethanol stimulus and their adaptive encapsulation capability towards optically active lanthanides. With the addition of ethanol to the Eu-TATAB nanorods, the structure was destroyed, resulting in a decrease in luminescence, electrochemiluminescence, and ultraviolet–visible spectrophotometric signals by perturbing energy transfer in the Eu-TATAB nanorods. According to the degree of the decrease in three luminescence intensities, the ethanol content could be quantitatively detected. Based on this, the rapid detection of alcohol content in different types of spirits and alcoholic beverages was further realized. This method is simple, convenient, and sensitive, and has good application prospects and development potential.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-21DOI: 10.1007/s00604-024-06881-5
Govinda Mandal, Muhammad Umar, Rui Lv, Ruochen Guo, Tianjin Ge, Muhammad Awais, Shunli Yang, Muhammad Sajjad Ul Hasan, Jian Liu
A new methodology is presented for the rapid, specific, and sensitive detection of irinotecan (CPT-11), a chemotherapeutic agent utilized in the treatment of cancer, along with its metabolically active derivative, SN-38, via laser desorption/ionization mass spectrometry (LDI MS). The method includes the detection of camptothecin (CPT), which can be utilized as an internal standard for the quantitative assessment of both CPT-11 and SN-38 in mouse serum. The approach utilizes a plasmonic two-dimensional (2D) black phosphorus nanosheet (BPN)-gold nanomatrix (BP@Au) in LDI MS. The experimental results demonstrated that the BP@Au nanomatrix outperformed the standard organic matrices (SA, CHCA, and DHB) in detecting irinotecan and its active metabolite with improved specificity and sensitivity, crucial factors for applications in personalized medicine. Mass spectra obtained using organic matrices revealed interference from matrix peaks overlapping with analyte peaks. The coefficient of determination (R2) was 0.9806 for CPT-11 and 0.9932 for SN-38, indicating strong linearity suitable for quantification. Moreover, the method achieved a lower limit of detection (LOD) of 62.76 ng/mL for CPT-11 and 189.87 ng/mL for SN-38, significantly enhancing the detection sensitivity by approximately 2–8 times compared with previous matrix-assisted laser desorption/ionization (MALDI) methodologies. This method was subsequently applied to the quantitative determination of analytes in mouse serum. The analyte recoveries for CPT-11 and SN-38 were 95.40% and 92.95%, respectively. Overall, this study offers potential insights and opens avenues for developing new nanomaterials as a MALDI nanomatrix, demonstrating enhanced capabilities for the rapid, specific, and sensitive detection of small biomolecules within the realms of analytical chemistry and personalized medicine.
{"title":"Facile synthesis of plasmonic BP@Au nanomatrix for sensitive detection of irinotecan and its active SN-38 metabolite via laser desorption/ionization mass spectrometry","authors":"Govinda Mandal, Muhammad Umar, Rui Lv, Ruochen Guo, Tianjin Ge, Muhammad Awais, Shunli Yang, Muhammad Sajjad Ul Hasan, Jian Liu","doi":"10.1007/s00604-024-06881-5","DOIUrl":"10.1007/s00604-024-06881-5","url":null,"abstract":"<div><p> A new methodology is presented for the rapid, specific, and sensitive detection of irinotecan (CPT-11), a chemotherapeutic agent utilized in the treatment of cancer, along with its metabolically active derivative, SN-38, via laser desorption/ionization mass spectrometry (LDI MS). The method includes the detection of camptothecin (CPT), which can be utilized as an internal standard for the quantitative assessment of both CPT-11 and SN-38 in mouse serum. The approach utilizes a plasmonic two-dimensional (2D) black phosphorus nanosheet (BPN)-gold nanomatrix (BP@Au) in LDI MS. The experimental results demonstrated that the BP@Au nanomatrix outperformed the standard organic matrices (SA, CHCA, and DHB) in detecting irinotecan and its active metabolite with improved specificity and sensitivity, crucial factors for applications in personalized medicine. Mass spectra obtained using organic matrices revealed interference from matrix peaks overlapping with analyte peaks. The coefficient of determination (<i>R</i><sup>2</sup>) was 0.9806 for CPT-11 and 0.9932 for SN-38, indicating strong linearity suitable for quantification. Moreover, the method achieved a lower limit of detection (LOD) of 62.76 ng/mL for CPT-11 and 189.87 ng/mL for SN-38, significantly enhancing the detection sensitivity by approximately 2–8 times compared with previous matrix-assisted laser desorption/ionization (MALDI) methodologies. This method was subsequently applied to the quantitative determination of analytes in mouse serum. The analyte recoveries for CPT-11 and SN-38 were 95.40% and 92.95%, respectively. Overall, this study offers potential insights and opens avenues for developing new nanomaterials as a MALDI nanomatrix, demonstrating enhanced capabilities for the rapid, specific, and sensitive detection of small biomolecules within the realms of analytical chemistry and personalized medicine.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-20DOI: 10.1007/s00604-025-06959-8
Man Du, Meimei Song, Shuang Hou, Yue Zhang, Haijun Lv, Shuchun Zhao, Hongxia Du, Hongyong Guo
A novel fluorescence sensing nanoplatform (CDs/AuNCs@ZIF-8) encapsulating carbon dots (CDs) and gold nanoclusters (AuNCs) within a zeolitic imidazolate framework-8 (ZIF-8) was developed for ratiometric detection of formaldehyde (FA) in the medium of hydroxylamine hydrochloride (NH2OH·HCl). The nanoplatform exhibited pink fluorescence due to the aggregation-induced emission (AIE) effect of AuNCs and the internal filtration effect (IFE) between AuNCs and CDs. Upon reaction between NH2OH·HCl and FA, a Schiff base formed via aldehyde-diamine condensation, releasing hydrochloric acid. The acid triggered the degradation of ZIF-8, releasing CDs and AuNCs, and thereby shifting the fluorescence to blue as the CDs disperse. The nanoplatform demonstrated high sensitivity (LOD of 0.26-2.19 μM), exceptional selectivity, and rapid response time (<1 min) toward FA. Additionally, test strips and hydrogel films integrated with smartphones were prepared for on-site and visual detection of FA. The portable and smartphone-assisted test strips effectively detected indoor FA gas, while wearable and intelligent hydrogel films provided reliable surface measurements of FA on fruits and vegetables. Real sample analyses achieved satisfactory FA recoveries (99.06-115.30%) with relative standard deviations (RSD) from 1.86% to 3.81%. The innovative sensing nanoplatform served as a promising approach for FA detection in food, construction materials, and indoor air quality, offering both analytical accuracy and convenient visualization.
{"title":"Metal-organic frameworks encapsulating gold nanoclusters and carbon dots for ratiometric fluorescent detection of formaldehyde in real food samples, construction materials and indoor environments","authors":"Man Du, Meimei Song, Shuang Hou, Yue Zhang, Haijun Lv, Shuchun Zhao, Hongxia Du, Hongyong Guo","doi":"10.1007/s00604-025-06959-8","DOIUrl":"10.1007/s00604-025-06959-8","url":null,"abstract":"<div><p> A novel fluorescence sensing nanoplatform (CDs/AuNCs@ZIF-8) encapsulating carbon dots (CDs) and gold nanoclusters (AuNCs) within a zeolitic imidazolate framework-8 (ZIF-8) was developed for ratiometric detection of formaldehyde (FA) in the medium of hydroxylamine hydrochloride (NH<sub>2</sub>OH·HCl). The nanoplatform exhibited pink fluorescence due to the aggregation-induced emission (AIE) effect of AuNCs and the internal filtration effect (IFE) between AuNCs and CDs. Upon reaction between NH<sub>2</sub>OH·HCl and FA, a Schiff base formed via aldehyde-diamine condensation, releasing hydrochloric acid. The acid triggered the degradation of ZIF-8, releasing CDs and AuNCs, and thereby shifting the fluorescence to blue as the CDs disperse. The nanoplatform demonstrated high sensitivity (LOD of 0.26-2.19 μM), exceptional selectivity, and rapid response time (<1 min) toward FA. Additionally, test strips and hydrogel films integrated with smartphones were prepared for on-site and visual detection of FA. The portable and smartphone-assisted test strips effectively detected indoor FA gas, while wearable and intelligent hydrogel films provided reliable surface measurements of FA on fruits and vegetables. Real sample analyses achieved satisfactory FA recoveries (99.06-115.30%) with relative standard deviations (RSD) from 1.86% to 3.81%. The innovative sensing nanoplatform served as a promising approach for FA detection in food, construction materials, and indoor air quality, offering both analytical accuracy and convenient visualization.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H2O2 from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.
{"title":"An ultrasensitive colorimetric/fluorescent/photothermal sensing platform for the detection of D-amino acids based on carbon dots nanozymes with enhanced peroxidase-like activity","authors":"Wei Wu, Chen Ni, Yan-Li Huang, Hui-Ling Cheng, Yu-Han Shi, Ya-Hui Zhang, Zhi-Hong Xu, Guo-Qi Zhang","doi":"10.1007/s00604-025-06961-0","DOIUrl":"10.1007/s00604-025-06961-0","url":null,"abstract":"<div><p>Based on the enhanced peroxidase-like activity of carbon dots nanozymes (CDszymes), with a specific oxidation reaction of D-amino acid oxidase catalysing the formation of H<sub>2</sub>O<sub>2</sub> from D-amino acid, an ultrasensitive sensing platform, was constructed for the quantitative detection of D-amino acids in saliva. With the increase of D-amino acids concentration, the blue color of catalytic product gradually deepend, the fluorescence CDszymes gradually quenched, and the temperature gradually increased. Using D-alanine as D-amino acid models, the detection limits of D-alanine in colorimetric/photothermal/fluorescent mode were 0.3 μM, 1.8 μM, and 0.04 μM, respectively. The proposed detection platform exhibits promising application potential in clinical diagnostics. The exceptional sensing performance can be attributed to the utilization of CDszymes with outstanding POD activity. Revolving around the blind synthesis of CDszymes exhibiting high-efficiency POD activity, this study delved into the underlying mechanism governing the regulation of the POD activity of CDszymes by precursor functional groups. This work investigates the valence band theory to enhance the peroxidase-like of CDszymes, thereby offering a rational approach for designing CDszymes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-18DOI: 10.1007/s00604-025-06950-3
Yuan Su, Ziyu Zhang, Hongfang Zhang
A biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS2 nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover, multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a G-quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron. In the absence of MiR-31, the tetrahedron DNAzyme probe formed after the addition of hemin can mediate the deposition of insoluble on MoS2/AAO, which not only enables the color change of the membrane but also gates the transport of K3[Fe(CN)6] across the nanochannels. Therefore, the detection of MiR-31 is realized by both visual observation of the brown color and measuring the electrochemical redox current of [Fe(CN)6]3−. Using this biosensor, a detection limit as low as 0.06 fM is achieved. The dual-mode detection method also exhibits good specificity, reproducibility, and stability, demonstrating potential application in the diagnosis of oral squamous cell carcinoma and other related biological purposes.
{"title":"A dual-mode biosensor for microRNA detection based on DNA tetrahedron-gated nanochannels","authors":"Yuan Su, Ziyu Zhang, Hongfang Zhang","doi":"10.1007/s00604-025-06950-3","DOIUrl":"10.1007/s00604-025-06950-3","url":null,"abstract":"<div><p>A biosensor based on solid-state nanochannels of anodic aluminum oxide (AAO) membrane for both electrochemical and naked-eye detection of microRNA-31 (MiR-31) is proposed. For this purpose, MoS<sub>2</sub> nanosheets, which possess different adsorption capabilities to single-stranded and double-stranded nucleic acids, are deposited onto the top surface of the AAO membrane. Moreover, multi-functional DNA nanostructure have been designed by linking a G-rich sequence for folding to a <b>G-</b>quadruplex at three vertices and a complementary sequence of MiR-31 at the other one vertex of a DNA tetrahedron. In the absence of MiR-31, the tetrahedron DNAzyme probe formed after the addition of hemin can mediate the deposition of insoluble on MoS<sub>2</sub>/AAO, which not only enables the color change of the membrane but also gates the transport of K<sub>3</sub>[Fe(CN)<sub>6</sub>] across the nanochannels. Therefore, the detection of MiR-31 is realized by both visual observation of the brown color and measuring the electrochemical redox current of [Fe(CN)<sub>6</sub>]<sup>3<b>−</b></sup>. Using this biosensor, a detection limit as low as 0.06 fM is achieved. The dual-mode detection method also exhibits good specificity, reproducibility, and stability, demonstrating potential application in the diagnosis of oral squamous cell carcinoma and other related biological purposes.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of Ag2S/FeOOH heterojunction. The Ag2S/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb. Mb-aptamer was used as the responsive group to grasp the target Mb in a real sample due to its advantages of strong affinity, high stability, and ease of preparation. Mb-aptamer immunocomplex is formed in the presence of Mb, which hinders the interfacial electron transfer and then reduce the photocurrent. The proposed PEC aptasensor exhibited excellent analytical performance including wide linear range (1.0 pg mL−1 ~ 50 ng mL−1), low limit of detection (0.28 pg mL−1), and good selectivity and stability. This work introduces an innovative approach to PEC aptasensor, offering a promising method for precise determination of human biomarkers.
Graphical Abstract
肌红蛋白（Mb）是一种重要的心脏标志物，在心血管疾病患者的诊断、监测和评估中起着至关重要的作用。在这里，我们提出了一种无标记的光电化学（PEC）传感器，通过靶调节Ag2S/FeOOH异质结的光活性来检测Mb。合成了Ag2S/FeOOH纳米纺锤体，作为构建Mb生物识别过程的传感平台。Mb-aptamer具有亲和力强、稳定性高、制备简单等优点，可作为响应基团在真实样品中捕获目标Mb。Mb-适体免疫复合物在Mb存在下形成，阻碍了界面电子转移，从而降低了光电流。该传感器线性范围宽（1.0 pg mL−1 ~ 50 ng mL−1），检出限低（0.28 pg mL−1），具有良好的选择性和稳定性。这项工作介绍了一种创新的PEC配体传感器方法，为精确测定人类生物标志物提供了一种有前途的方法。图形抽象
{"title":"Target-regulated Ag2S/FeOOH heterojunction activity: a direct label-free photoelectrochemical immunosensor","authors":"Rui-Jin Shao, Min-Li Wang, Yadong Xue, Na Zhang, Ai-Jun Wang, Pei Song, Li-Ping Mei, Jiu-Ju Feng","doi":"10.1007/s00604-024-06929-6","DOIUrl":"10.1007/s00604-024-06929-6","url":null,"abstract":"<div><p>Myoglobin (Mb), an important cardiac marker, plays a crucial role in diagnosing, monitoring, and evaluating the condition of patients with cardiovascular diseases. Here, we propose a label-free photoelectrochemical (PEC) sensor for the detection of Mb through target regulated the photoactivity of Ag<sub>2</sub>S/FeOOH heterojunction. The Ag<sub>2</sub>S/FeOOH nanospindles were synthesized and served as a sensing platform for the fabrication of bio-recognized process for Mb. Mb-aptamer was used as the responsive group to grasp the target Mb in a real sample due to its advantages of strong affinity, high stability, and ease of preparation. Mb-aptamer immunocomplex is formed in the presence of Mb, which hinders the interfacial electron transfer and then reduce the photocurrent. The proposed PEC aptasensor exhibited excellent analytical performance including wide linear range (1.0 pg mL<sup>−1</sup> ~ 50 ng mL<sup>−1</sup>), low limit of detection (0.28 pg mL<sup>−1</sup>), and good selectivity and stability. This work introduces an innovative approach to PEC aptasensor, offering a promising method for precise determination of human biomarkers.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995068","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1007/s00604-024-06937-6
Keao Liu, Fengshan Liu, Yang Xu
A kind of sulfur-doped carbon dots was prepared which were encapsulated with polydopamine (S-CDs@PDA) that has fluorescence response on polyethylene (PE) microplastics (MPs). Modified membranes were constructed using S-CDs@PDA for MP detection. Through heating and vacuum filtration process, yellow emission from the modified membrane appeared because of the combination between S-CDs@PDA and PE MPs. Notably, the fluorescence signal value of PE MPs detected by S-CDs@PDA-modified membrane was 21.3% higher than that of unmodified S-CDs membrane, and the detection efficiency was 8% higher than that of S-CDs membrane. The minimum detection limit for modified membranes was 4 mg. Due to the good adhesion of polydopamine (PDA), S-CDs@PDA-modified membrane was more easily adhered to PE MPs, showing its excellent detection ability. The rapid quantitative detection of PE MPs in 10 min was realized with a linear equation of y = 3081x + 3686.1 in a linear range of 4–14 mg. Such modified membrane exhibited excellent anti-photobleaching using continuous 365-nm excitation and its sensing performance was further confirmed in sea and river water samples. S-CDs@PDA could detect solid MP powders as well as MPs dispersed in liquids. The detection method constructed with a modified glass fiber filter membrane enables rapid identification and quantitative detection of PE MPs without relying on large-scale instrumentation. This study provided research ideas for fluorescent tracing of PE MPs and paved the way for quantitative detection of micron-sized plastics with smaller particle sizes.
{"title":"Polydopamine-encapsulated carbon dots to boost analytical performance for microplastics detection in fluorescence mode","authors":"Keao Liu, Fengshan Liu, Yang Xu","doi":"10.1007/s00604-024-06937-6","DOIUrl":"10.1007/s00604-024-06937-6","url":null,"abstract":"<div><p>A kind of sulfur-doped carbon dots was prepared which were encapsulated with polydopamine (S-CDs@PDA) that has fluorescence response on polyethylene (PE) microplastics (MPs). Modified membranes were constructed using S-CDs@PDA for MP detection. Through heating and vacuum filtration process, yellow emission from the modified membrane appeared because of the combination between S-CDs@PDA and PE MPs. Notably, the fluorescence signal value of PE MPs detected by S-CDs@PDA-modified membrane was 21.3% higher than that of unmodified S-CDs membrane, and the detection efficiency was 8% higher than that of S-CDs membrane. The minimum detection limit for modified membranes was 4 mg. Due to the good adhesion of polydopamine (PDA), S-CDs@PDA-modified membrane was more easily adhered to PE MPs, showing its excellent detection ability. The rapid quantitative detection of PE MPs in 10 min was realized with a linear equation of <i>y</i> = 3081<i>x</i> + 3686.1 in a linear range of 4–14 mg. Such modified membrane exhibited excellent anti-photobleaching using continuous 365-nm excitation and its sensing performance was further confirmed in sea and river water samples. S-CDs@PDA could detect solid MP powders as well as MPs dispersed in liquids. The detection method constructed with a modified glass fiber filter membrane enables rapid identification and quantitative detection of PE MPs without relying on large-scale instrumentation. This study provided research ideas for fluorescent tracing of PE MPs and paved the way for quantitative detection of micron-sized plastics with smaller particle sizes.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995439","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-17DOI: 10.1007/s00604-024-06889-x
Hyun Pyo Kim, Ji-Ho Park, Min-Gon Kim, Youngung Seok
The global healthcare market increasingly demands affordable molecular diagnostics for field testing. To address this need, we introduce a lab-on-paper (LOP) platform that integrates isothermal amplification with a specially designed paper strip for molecular testing through an automated microfluidics process. The LOP system is engineered for rapid, cost-effective, and highly sensitive detection, using USB-powered thermal management and a wax valve mechanism. This innovative platform provides an accessible solution for the rapid and accurate detection of various microorganisms, proving particularly advantageous for point-of-care testing in resource-limited environments. Experiments conducted in this study demonstrated the efficacy of the LOP platform in the colorimetric detection of foodborne pathogens. It reliably detected Vibrio vulnificus at concentrations as low as 120 CFU/mL and Salmonella Typhimurium at 60 CFU/mL, with results observable to the naked eye. The entire process, encompassing amplification and detection, was completed within 30 min, underscoring the system’s rapid diagnostic capability. Furthermore, with an assay cost of 5.2 USD per test, the platform offers a highly cost-effective solution for molecular diagnostics, particularly in resource-limited settings. The LOP platform’s portability, ease of use, and affordability make it a promising alternative for various diagnostic applications, including infectious disease monitoring and ensuring food safety.
{"title":"Lab-on-paper for molecular testing with USB-powered isothermal amplification and fluidic control","authors":"Hyun Pyo Kim, Ji-Ho Park, Min-Gon Kim, Youngung Seok","doi":"10.1007/s00604-024-06889-x","DOIUrl":"10.1007/s00604-024-06889-x","url":null,"abstract":"<div><p>The global healthcare market increasingly demands affordable molecular diagnostics for field testing. To address this need, we introduce a lab-on-paper (LOP) platform that integrates isothermal amplification with a specially designed paper strip for molecular testing through an automated microfluidics process. The LOP system is engineered for rapid, cost-effective, and highly sensitive detection, using USB-powered thermal management and a wax valve mechanism. This innovative platform provides an accessible solution for the rapid and accurate detection of various microorganisms, proving particularly advantageous for point-of-care testing in resource-limited environments. Experiments conducted in this study demonstrated the efficacy of the LOP platform in the colorimetric detection of foodborne pathogens. It reliably detected <i>Vibrio vulnificus</i> at concentrations as low as 120 CFU/mL and <i>Salmonella Typhimurium</i> at 60 CFU/mL, with results observable to the naked eye. The entire process, encompassing amplification and detection, was completed within 30 min, underscoring the system’s rapid diagnostic capability. Furthermore, with an assay cost of 5.2 USD per test, the platform offers a highly cost-effective solution for molecular diagnostics, particularly in resource-limited settings. The LOP platform’s portability, ease of use, and affordability make it a promising alternative for various diagnostic applications, including infectious disease monitoring and ensuring food safety.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 2","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142995473","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-01-16DOI: 10.1007/s00604-024-06944-7
Mohammad Beiranvand, Gholamreza Dehghan
MILs (Materials Institute Lavoisier), as nanocarriers based on metal–organic frameworks (MOFs), are one of the most advanced drug delivery vehicles that are now a major part of cancer treatment research. This review article highlights the key features and components of MIL nanocarriers for the development and improvement of these nanocarriers for drug delivery. Surface coatings are one of the key components of MIL nanocarriers, which play the role of stabilizing the nanocarrier, pH-dependent drug release, increasing the half-life of the drug, and targeting the carrier. MIL nanocarriers have been synthesized mainly by thermal and hydrothermal methods due to their single-step nature and the ability to produce individual crystals with tunable sizes. According to the data available in the literature, MIL-53 and MIL-101 are the best MILs for drug delivery. These MILs have a high ability to release drugs under acidic conditions, indicating their high efficiency compared to other MILs. In addition to drugs, these nanocarriers can also carry fluorescent, photothermal, and photodynamic agents. These agents allow the MIL nanocarriers to benefit from the therapeutic potential of photothermal and photodynamic agents in addition to the therapeutic capacity of the drug. Furthermore, the fluorescent active ingredient gives these nanocarriers a further tracking capability in addition to the inherent tracking capability of MRI. Therefore, MIL nanocarriers as theranostic carriers have the potential to revolutionize both drug delivery and imaging.
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