Pub Date : 2026-01-02DOI: 10.1007/s00604-025-07797-4
Çağdaş Salih Meriç, Bahar Bankoğlu Yola, Sena Bekerecioğlu, İlknur Polat, Necip Atar, Mehmet Lütfi Yola
A new type quartz crystal microbalance (QCM) sensor based on nitrogen sulphur co-doped carbon dots incorporated TiO2 nanoparticles (NSCTi) nanocomposite and molecularly imprinting polymers (MIPs) is presented for acetamiprid (ACE) detection. After the preparation of NSCTi nanocomposite by using microwave irradiation treatment and the hydrothermal method, QCM chip modified NSCTi nanocomposite was developed by using the interaction between gold and sulphur. Then, MIPs based on QCM chip modified NSCTi nanocomposite was designed via the spin coating method providing mono-layered and homogeneous QCM surface in presence of methacryloylamidoglutamic acid (MAGA) as monomer and N, N′-azobisisobutyronitrile (AIBN) as initiator. After the characterization investigations, the linearity in the range 1.0 × 10− 9 – 2.0 × 10− 8 M with a detection limit (LOD) of 3.3 × 10− 10 M was calculated for ACE. Finally, the developed QCM sensor was applied to wastewater samples with high recovery.
{"title":"A novel insecticide acetamiprid detection in wastewater samples by quartz crystal microbalance sensor based on molecularly imprinting polymer and nitrogen sulphur co-doped carbon dots incorporated TiO2 nanoparticles","authors":"Çağdaş Salih Meriç, Bahar Bankoğlu Yola, Sena Bekerecioğlu, İlknur Polat, Necip Atar, Mehmet Lütfi Yola","doi":"10.1007/s00604-025-07797-4","DOIUrl":"10.1007/s00604-025-07797-4","url":null,"abstract":"<div><p>A new type quartz crystal microbalance (QCM) sensor based on nitrogen sulphur co-doped carbon dots incorporated TiO<sub>2</sub> nanoparticles (NSCTi) nanocomposite and molecularly imprinting polymers (MIPs) is presented for acetamiprid (ACE) detection. After the preparation of NSCTi nanocomposite by using microwave irradiation treatment and the hydrothermal method, QCM chip modified NSCTi nanocomposite was developed by using the interaction between gold and sulphur. Then, MIPs based on QCM chip modified NSCTi nanocomposite was designed via the spin coating method providing mono-layered and homogeneous QCM surface in presence of methacryloylamidoglutamic acid (MAGA) as monomer and N, N′-azobisisobutyronitrile (AIBN) as initiator. After the characterization investigations, the linearity in the range 1.0 × 10<sup>− 9</sup> – 2.0 × 10<sup>− 8</sup> M with a detection limit (LOD) of 3.3 × 10<sup>− 10</sup> M was calculated for ACE. Finally, the developed QCM sensor was applied to wastewater samples with high recovery.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1007/s00604-025-07729-2
Yilong Xing, Shengnan Liu, Xiangshan Yang, Juan Li, Rui Zhang
Polyethyleneimine (PEI) modified 5,10,15,20-tetrakis(4-(hydroxyl)phenyl)porphyrin (THPP) assemblies with different morphologies are prepared via an evaporation-induced self-assembly method. The obtained THPP/PEI assemblies have positive surface potentials under physiological conditions. Therefore, THPP/PEI assemblies can capture extracellular vesicles (EVs) from MCF-7 cells via electrostatic interaction. Furthermore, THPP/PEI assemblies can also isolate EVs in size according to their different morphologies. The EV capture efficiency of THPP/PEI assembly is much high than those of inorganic ZnO-based nanowire arrays, although both rely on electrostatic attraction to capture EVs. Using the THPP/PEI assemblies, we demonstrated that the expression level of CD147 of EVs derived from the urine samples of colon cancer patients was 4.3-fold higher than that from the urine samples of healthy donors with a p-value less than 0.01. It is expected that the new THPP/PEI assemblies in this work has potential to be applied to liquid biopsy technology because of their portability and cost effectiveness.
{"title":"Porphyrin micro–nano assemblies for capture and isolation of extracellular vesicles","authors":"Yilong Xing, Shengnan Liu, Xiangshan Yang, Juan Li, Rui Zhang","doi":"10.1007/s00604-025-07729-2","DOIUrl":"10.1007/s00604-025-07729-2","url":null,"abstract":"<div><p>Polyethyleneimine (PEI) modified 5,10,15,20-tetrakis(4-(hydroxyl)phenyl)porphyrin (THPP) assemblies with different morphologies are prepared <i>via</i> an evaporation-induced self-assembly method. The obtained THPP/PEI assemblies have positive surface potentials under physiological conditions. Therefore, THPP/PEI assemblies can capture extracellular vesicles (EVs) from MCF-7 cells via electrostatic interaction. Furthermore, THPP/PEI assemblies can also isolate EVs in size according to their different morphologies. The EV capture efficiency of THPP/PEI assembly is much high than those of inorganic ZnO-based nanowire arrays, although both rely on electrostatic attraction to capture EVs. Using the THPP/PEI assemblies, we demonstrated that the expression level of CD147 of EVs derived from the urine samples of colon cancer patients was 4.3-fold higher than that from the urine samples of healthy donors with a <i>p</i>-value less than 0.01. It is expected that the new THPP/PEI assemblies in this work has potential to be applied to liquid biopsy technology because of their portability and cost effectiveness.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1007/s00604-025-07789-4
Zhaojiang Yin, Jing Zhang, Binghui Li, Hao Fan, Mei Wang, Hanfeng Cui
A dual-mode sensor based on intramolecular catalytic hairpin assembly strategy (intra-CHA) is proposed for Survivin mRNA detection. Intra-CHA, a triplex complex of tetraferrocene-labeled H1/H2, shortens H1-H2 distance and increases their local concentrations, accelerating reaction by 2.8-fold vs. free-CHA. Ruthenium-based covalent organic frameworks (RuCOFs) with Ru(dcbpy)32+ as luminophore has dual fluorescence-electrochemiluminescence (FL-ECL) properties. EDTA-incorporated RuCOFs@EDTA expands active sites, enhancing ECL efficiency 8-fold. Intra-CHA system is covalently conjugated to RuCOFs@EDTA through amide bond linkages. Target mRNA-triggered strand displacement reactions induce structural reconfiguration of the intra-CHA probes, resulting in controlled spatial segregation of tetraferrocene from the RuCOFs@EDTA interface. This results in the recovery of FL and ECL signals, enabling rapid and highly sensitive detection of Survivin mRNA. Experimental results show that the detection limits reach 0.04 fM (in the FL mode) and 0.009 fM (in the ECL mode) respectively, with excellent selectivity, stability, and reproducibility. The system has been successfully applied to detect Survivin mRNA in actual serum samples, providing a novel strategy for Survivin mRNA detection and paving the way for developing future diagnostic strategies for early cancer screening.
Graphical Abstract
提出了一种基于分子内催化发夹组装策略(intra-CHA)的双模传感器用于Survivin mRNA检测。cha内是四铁二烯标记的H1/H2的三重复合物,缩短了H1-H2的距离,增加了它们的局部浓度,比游离cha的反应速度加快了2.8倍。以Ru(dcbpy)32+为发光团的钌基共价有机骨架(RuCOFs)具有荧光-电化学发光(FL-ECL)双特性。edta合并RuCOFs@EDTA扩展活性位点,提高ECL效率8倍。cha内系统通过酰胺键键共价共轭到RuCOFs@EDTA。靶mrna触发的链位移反应诱导cha内探针的结构重构,导致四茂铁从RuCOFs@EDTA界面的可控空间分离。这导致FL和ECL信号的恢复,从而能够快速和高灵敏度地检测Survivin mRNA。实验结果表明,该方法的检出限分别达到0.04 fM (FL模式)和0.009 fM (ECL模式),具有良好的选择性、稳定性和重复性。该系统已成功应用于实际血清样本中Survivin mRNA的检测,为Survivin mRNA的检测提供了一种新的策略,为未来早期癌症筛查的诊断策略的发展铺平了道路。图形抽象
{"title":"A dual-mode sensor based on an intramolecular catalytic hairpin assembly strategy for detecting survivin mRNA","authors":"Zhaojiang Yin, Jing Zhang, Binghui Li, Hao Fan, Mei Wang, Hanfeng Cui","doi":"10.1007/s00604-025-07789-4","DOIUrl":"10.1007/s00604-025-07789-4","url":null,"abstract":"<div><p> A dual-mode sensor based on intramolecular catalytic hairpin assembly strategy (intra-CHA) is proposed for Survivin mRNA detection. Intra-CHA, a triplex complex of tetraferrocene-labeled H1/H2, shortens H1-H2 distance and increases their local concentrations, accelerating reaction by 2.8-fold vs. free-CHA. Ruthenium-based covalent organic frameworks (RuCOFs) with Ru(dcbpy)<sub>3</sub><sup>2+</sup> as luminophore has dual fluorescence-electrochemiluminescence (FL-ECL) properties. EDTA-incorporated RuCOFs@EDTA expands active sites, enhancing ECL efficiency 8-fold. Intra-CHA system is covalently conjugated to RuCOFs@EDTA through amide bond linkages. Target mRNA-triggered strand displacement reactions induce structural reconfiguration of the intra-CHA probes, resulting in controlled spatial segregation of tetraferrocene from the RuCOFs@EDTA interface. This results in the recovery of FL and ECL signals, enabling rapid and highly sensitive detection of Survivin mRNA. Experimental results show that the detection limits reach 0.04 fM (in the FL mode) and 0.009 fM (in the ECL mode) respectively, with excellent selectivity, stability, and reproducibility. The system has been successfully applied to detect Survivin mRNA in actual serum samples, providing a novel strategy for Survivin mRNA detection and paving the way for developing future diagnostic strategies for early cancer screening.</p><h3>Graphical Abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2026-01-02DOI: 10.1007/s00604-025-07748-z
Alberto Escarpa, Mamas Prodromidis
{"title":"Topical collection in memoriam Otto S. Wolfbeis (1947–2023)","authors":"Alberto Escarpa, Mamas Prodromidis","doi":"10.1007/s00604-025-07748-z","DOIUrl":"10.1007/s00604-025-07748-z","url":null,"abstract":"","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Biomass-derived nitrogen self-doped carbon quantum dots (N-CQDs) were synthesized by the hydrothermal method using soybean residue as both nitrogen and carbon sources. The N-CQDs were applied to the study of cell multicolor imaging and temperature sensing in living cells. The experimental results show that N-CQDs have strong fluorescence intensity, good biocompatibility, good cell membrane penetration, good cell imaging performance and strong temperature sensitivity. In the temperature range 25 ~ 60℃, the intensity of the N-CQDs decreased with increase of temperature, and showed a good linear relationship. At 60℃, the relative fluorescence intensity was quenched by 65%. The results show that the soybean residue N-CQDs have high application potential and value in the field of fluorescence temperature sensing in living cells, and are of great significance for the development of nondestructive cell measurement nanothermometers. It is expected that the N-CQDs may establish as a temperature-sensitive fluorescence nanoprobe widely used in biomedical fields such as cell research.
{"title":"Synthesis of biomass nitrogen self-doped carbon quantum dots and their application in multicolor imaging and temperature sensing in cells","authors":"Yue‑Fang Hu, Yu‑Xin Chen, Ting Chen, Jin-Fang Li, Sheng-Yu Chen","doi":"10.1007/s00604-025-07741-6","DOIUrl":"10.1007/s00604-025-07741-6","url":null,"abstract":"<div><p>Biomass-derived nitrogen self-doped carbon quantum dots (N-CQDs) were synthesized by the hydrothermal method using soybean residue as both nitrogen and carbon sources. The N-CQDs were applied to the study of cell multicolor imaging and temperature sensing in living cells. The experimental results show that N-CQDs have strong fluorescence intensity, good biocompatibility, good cell membrane penetration, good cell imaging performance and strong temperature sensitivity. In the temperature range 25 ~ 60℃, the intensity of the N-CQDs decreased with increase of temperature, and showed a good linear relationship. At 60℃, the relative fluorescence intensity was quenched by 65%. The results show that the soybean residue N-CQDs have high application potential and value in the field of fluorescence temperature sensing in living cells, and are of great significance for the development of nondestructive cell measurement nanothermometers. It is expected that the N-CQDs may establish as a temperature-sensitive fluorescence nanoprobe widely used in biomedical fields such as cell research.</p><h3>Graphical abstract</h3><div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"193 1","pages":""},"PeriodicalIF":5.3,"publicationDate":"2026-01-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145886962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}