A differential pulse voltammetric (DPV) method is proposed for the highly sensitive determination of fenhexamid (FHX) based on both electrooxidation and electroreduction processes using a disposable and cost-effective pencil graphite electrode (PGE). The electrochemical oxidation and reduction mechanisms of FHX at the PGE were elucidated by recording cyclic voltammograms at various pH values of Britton–Robinson buffer (BRB) solutions at a scan rate of 50 mV s−1 and different scan rate values in the range 10–400 mV s−1 at selected pH of BRB (pH 2.0). Differential pulse voltammograms recorded under optimized conditions revealed an oxidation peak of FHX around + 0.65 V and a reduction peak of FHX around + 0.45 V. The DPV analysis of FHX revealed two linear ranges: 0.001–0.01 µmol L−1 and 0.01–5.0 µmol L−1 for the anodic peak, and 0.001–0.1 µmol L−1 and 0.1–5.0 µmol L−1 for the cathodic peak. The limits of detection were 0.34 nmol L−1 and 0.32 nmol L−1 for the anodic and cathodic peaks, respectively. The proposed methodology demonstrated satisfactory selectivity, as selected pesticides, certain electroactive compounds, and cationic species tested did not interfere with the voltammetric determination of FHX, particularly during its reduction. The recovery results, showing values close to 100% obtained from the analysis of real samples spiked with FHX, indicated that this methodology can accurately determine FHX in water and soil samples.
Graphical abstract
{"title":"Highly sensitive voltammetric determination of the fungicide fenhexamid using a cost-effective and disposable pencil graphite electrode","authors":"Teslime Erşan, Didem Giray Dilgin, Ayhan Oral, Sławomira Skrzypek, Mariola Brycht, Yusuf Dilgin","doi":"10.1007/s00604-024-06804-4","DOIUrl":"10.1007/s00604-024-06804-4","url":null,"abstract":"<div><p> A differential pulse voltammetric (DPV) method is proposed for the highly sensitive determination of fenhexamid (FHX) based on both electrooxidation and electroreduction processes using a disposable and cost-effective pencil graphite electrode (PGE). The electrochemical oxidation and reduction mechanisms of FHX at the PGE were elucidated by recording cyclic voltammograms at various pH values of Britton–Robinson buffer (BRB) solutions at a scan rate of 50 mV s<sup>−1</sup> and different scan rate values in the range 10–400 mV s<sup>−1</sup> at selected pH of BRB (pH 2.0). Differential pulse voltammograms recorded under optimized conditions revealed an oxidation peak of FHX around + 0.65 V and a reduction peak of FHX around + 0.45 V. The DPV analysis of FHX revealed two linear ranges: 0.001–0.01 µmol L<sup>−1</sup> and 0.01–5.0 µmol L<sup>−1</sup> for the anodic peak, and 0.001–0.1 µmol L<sup>−1</sup> and 0.1–5.0 µmol L<sup>−1</sup> for the cathodic peak. The limits of detection were 0.34 nmol L<sup>−1</sup> and 0.32 nmol L<sup>−1</sup> for the anodic and cathodic peaks, respectively. The proposed methodology demonstrated satisfactory selectivity, as selected pesticides, certain electroactive compounds, and cationic species tested did not interfere with the voltammetric determination of FHX, particularly during its reduction. The recovery results, showing values close to 100% obtained from the analysis of real samples spiked with FHX, indicated that this methodology can accurately determine FHX in water and soil samples.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06804-4.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142749536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-29DOI: 10.1007/s00604-024-06841-z
Xue Wang, Zixuan Zhao, Long An, Tianxiang Wang, Xiaojing Yang, Jiajia Shan
An unlabeled electrochemical sensing strategy based on electroactive copper-centered metal–organic framework (Cu-MOF) film coupled with multiwalled carbon nanotubes (MWCNTs) was proposed for the rapid assessment of nanoplastic concentration. The sensing interface was fabricated via the electro-deposition of Cu-MOF on the pre-modified MWCNTs using the cathodic reduction method. The exposed copper active sites in Cu-MOF showed excellent electrochemical activity, which was further enhanced due to rapid electron transfer induced by highly conductive MWCNTs. Through the adsorption functionality of Cu-MOF film towards polystyrene (PS) nanoplastics, the rapid recognition for nanoplastics in aqueous solution was achieved, thereby causing the inhibition of the current response. The results showed a robust dependence of the inhibition rate on the PS mass concentration. The proposed detection method was used for the quantitative determination of PS nanoplastics with the sizes of 100 nm, 500 nm, and 1 μm. The applicability of this electrochemical sensing platform was successfully validated in real-world water sample analysis.
{"title":"Novel unlabeled electrochemical sensing platform based on highly electroactive Cu-MOF film for nanoplastic detection in water","authors":"Xue Wang, Zixuan Zhao, Long An, Tianxiang Wang, Xiaojing Yang, Jiajia Shan","doi":"10.1007/s00604-024-06841-z","DOIUrl":"10.1007/s00604-024-06841-z","url":null,"abstract":"<div><p>An unlabeled electrochemical sensing strategy based on electroactive copper-centered metal–organic framework (Cu-MOF) film coupled with multiwalled carbon nanotubes (MWCNTs) was proposed for the rapid assessment of nanoplastic concentration. The sensing interface was fabricated via the electro-deposition of Cu-MOF on the pre-modified MWCNTs using the cathodic reduction method. The exposed copper active sites in Cu-MOF showed excellent electrochemical activity, which was further enhanced due to rapid electron transfer induced by highly conductive MWCNTs. Through the adsorption functionality of Cu-MOF film towards polystyrene (PS) nanoplastics, the rapid recognition for nanoplastics in aqueous solution was achieved, thereby causing the inhibition of the current response. The results showed a robust dependence of the inhibition rate on the PS mass concentration. The proposed detection method was used for the quantitative determination of PS nanoplastics with the sizes of 100 nm, 500 nm, and 1 μm. The applicability of this electrochemical sensing platform was successfully validated in real-world water sample analysis.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1007/s00604-024-06790-7
Kirill Yu. Presnyakov, Polina M. Ilicheva, Daria V. Tsyupka, Ekaterina A. Khudina, Mikhail V. Pozharov, Pavel S. Pidenko, Natalia A. Burmistrova
The aim of this study is to develop molecularly imprinted protein specific to zearalenone (ZEN). The primary idea of our study was to replace the toxic template—ZEN—with a dummy-template—4–hydroxicoumarin—during the synthesis of imprinted proteins (IPs). The choice of the dummy-template was based on the results of comprehensive evaluation that included a combination of blind docking and molecular dynamics simulations. Furthermore, we studied the influence of protonation and purification conditions to IPs sorption capacity. 3D fluorescence spectroscopy was used to monitor the process of bovine serum albumin (BSA) imprinting. The modified purification approach allowed reducing the eluent volume and purification time by approximately 300 and 48 times, respectively. The imprinted BSA was then used to produce a bioinorganic sorbent (IPs–BIS) based on silica nanoparticles (silica NPs), that, as far as we know, was never described before. The synthesized IPs–BIS were successfully applied as ZEN sorbents in model solutions (Q(_text {max}) = 1.70 ± 0.15 mg g(^{-1}), imprinting factor = 2.5) and artificially contaminated wheat extract (Q(_text {max}) = 2.24 ± 0.02 mg g(^{-1})) confirmed by HPLC–UV. We believe that our method can be used for mycotoxin monitoring in animal feeds and foodstuff.
Dummy-template imprinted bovine serum albumin for extraction of zearalenone
{"title":"Dummy-template imprinted bovine serum albumin for extraction of zearalenone","authors":"Kirill Yu. Presnyakov, Polina M. Ilicheva, Daria V. Tsyupka, Ekaterina A. Khudina, Mikhail V. Pozharov, Pavel S. Pidenko, Natalia A. Burmistrova","doi":"10.1007/s00604-024-06790-7","DOIUrl":"10.1007/s00604-024-06790-7","url":null,"abstract":"<p>The aim of this study is to develop molecularly imprinted protein specific to zearalenone (ZEN). The primary idea of our study was to replace the toxic template—ZEN—with a dummy-template—4–hydroxicoumarin—during the synthesis of imprinted proteins (IPs). The choice of the dummy-template was based on the results of comprehensive evaluation that included a combination of blind docking and molecular dynamics simulations. Furthermore, we studied the influence of protonation and purification conditions to IPs sorption capacity. 3D fluorescence spectroscopy was used to monitor the process of bovine serum albumin (BSA) imprinting. The modified purification approach allowed reducing the eluent volume and purification time by approximately 300 and 48 times, respectively. The imprinted BSA was then used to produce a bioinorganic sorbent (IPs–BIS) based on silica nanoparticles (silica NPs), that, as far as we know, was never described before. The synthesized IPs–BIS were successfully applied as ZEN sorbents in model solutions (Q<span>(_text {max})</span> = 1.70 ± 0.15 mg g<span>(^{-1})</span>, imprinting factor = 2.5) and artificially contaminated wheat extract (Q<span>(_text {max})</span> = 2.24 ± 0.02 mg g<span>(^{-1})</span>) confirmed by HPLC–UV. We believe that our method can be used for mycotoxin monitoring in animal feeds and foodstuff.</p><p>Dummy-template imprinted bovine serum albumin for extraction of zearalenone</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736817","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1007/s00604-024-06850-y
Nimisha Jadon, Ahmet Cetinkaya, Goksu Ozcelikay-Akyildiz, S. Irem Kaya, Esen Bellur Atici, Lokman Uzun, Sibel A. Ozkan
N-nitrosodimethylamine (NDMA) was determined using a molecularly imprinted polymer (MIP)-based electrochemical sensor. Green-synthesized silver nanoparticles were functionalized with cysteamine to enhance their integration into the electrode surface, which was used to modify a glassy carbon electrode (GCE). Furthermore, a MIP-based electrochemical sensor was constructed via electropolymerization of 3-aminophenyl boronic acid (3-APBA) as a conjugated functional monomer in the presence of lithium perchlorate (LiClO4) solution as a dopant, chitosan as a carrier natural polymer, and NDMA as a template/target molecule. The polymer film was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The analytical performance of the silver nanomaterial-based MIP-based electrochemical (AgNPs@Chitosan/3-APBA@MIP-GCE) sensor was evaluated under optimized conditions. The linear range of NDMA was 1.0 × 10–13–1.0 × 10–12 M (0.1–1.0 pM), with a limit of detection (LOD) of 3.63 × 10–15 M (3.63 fM) using differential pulse voltammetry (DPV). Method validation figured out that the developed MIP-based electrochemical nanosensor exhibited excellent selectivity, accuracy, and precision, which was shown by the analysis of synthetic serum samples and tap water. The LOD and LOQ in serum samples were 17.8 fM and 59.5 fM, respectively, which were in agreement with the developed method. Good recovery results confirm the successful application of the method in serum and tap water samples. The selectivity of the developed AgNPs@Chitosan/3-APBA@MIP-GCE sensor for NDMA was demonstrated in the presence of NDEA, sartans (valsartan, losartan, irbesartan, candesartan, telmisartan), and potential interferents that are possibly present in biological fluids (dopamine, ascorbic acid, uric acid) besides ionic species (sodium, chloride, potassium, nitrate, magnesium, sulfate) and common analgesic paracetamol.
{"title":"Development of a green-synthesized molecularly imprinted polymer-based electrochemical nanosensor for the determination of N-nitrosodimethylamine (NDMA) in serum and tap water","authors":"Nimisha Jadon, Ahmet Cetinkaya, Goksu Ozcelikay-Akyildiz, S. Irem Kaya, Esen Bellur Atici, Lokman Uzun, Sibel A. Ozkan","doi":"10.1007/s00604-024-06850-y","DOIUrl":"10.1007/s00604-024-06850-y","url":null,"abstract":"<div><p>N-nitrosodimethylamine (NDMA) was determined using a molecularly imprinted polymer (MIP)-based electrochemical sensor. Green-synthesized silver nanoparticles were functionalized with cysteamine to enhance their integration into the electrode surface, which was used to modify a glassy carbon electrode (GCE). Furthermore, a MIP-based electrochemical sensor was constructed via electropolymerization of 3-aminophenyl boronic acid (3-APBA) as a conjugated functional monomer in the presence of lithium perchlorate (LiClO<sub>4</sub>) solution as a dopant, chitosan as a carrier natural polymer, and NDMA as a template/target molecule. The polymer film was characterized by scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS). The analytical performance of the silver nanomaterial-based MIP-based electrochemical (AgNPs@Chitosan/3-APBA@MIP-GCE) sensor was evaluated under optimized conditions. The linear range of NDMA was 1.0 × 10<sup>–13</sup>–1.0 × 10<sup>–12</sup> M (0.1–1.0 pM), with a limit of detection (LOD) of 3.63 × 10<sup>–15</sup> M (3.63 fM) using differential pulse voltammetry (DPV). Method validation figured out that the developed MIP-based electrochemical nanosensor exhibited excellent selectivity, accuracy, and precision, which was shown by the analysis of synthetic serum samples and tap water. The LOD and LOQ in serum samples were 17.8 fM and 59.5 fM, respectively, which were in agreement with the developed method. Good recovery results confirm the successful application of the method in serum and tap water samples. The selectivity of the developed AgNPs@Chitosan/3-APBA@MIP-GCE sensor for NDMA was demonstrated in the presence of NDEA, sartans (valsartan, losartan, irbesartan, candesartan, telmisartan), and potential interferents that are possibly present in biological fluids (dopamine, ascorbic acid, uric acid) besides ionic species (sodium, chloride, potassium, nitrate, magnesium, sulfate) and common analgesic paracetamol.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-28DOI: 10.1007/s00604-024-06844-w
Yanping Chang, Jingyi Wang, Hongwei Guo, Wanqing Yao, Haijiao Xie, Long Li, Xianhu Liu
The synthesis of a novel bidentate ligand-protected copper nanocluster via a solid-state strategy is reported. Single-crystal X-ray diffraction analysis result reveals that the copper nanocluster features an octahedral core (Cu6) coordinated by six ligands. Noncovalent interactions (C-H…π and π…π) exist between the copper nanoclusters. The copper nanocluster displays luminescence even at 250 °C. The luminescence intensity is linearly correlated with temperature changes. The copper nanocluster can assemble into luminescent nanosheets whose emission is quenched by 4-nitrophenol. Spectroscopic analysis and theoretical calculations results demonstrate that the inner filter effect and electron transfer cause the above quenching effect. A probe based on luminescent nanosheets was constructed for β-galactosidase activity determination. The linearity range is 3.3–91.8 U·L−1, and the limit of detection is 0.45 U·L−1. This probe was also evaluated for determination of the β-galactosidase activity in human serum via spiking experiments. The recoveries ranged from 96.2% to 101.8%.
Graphical Abstract
报告采用固态策略合成了一种新型双齿配体保护的纳米铜簇。单晶 X 射线衍射分析结果表明，纳米铜簇具有一个八面体核心（Cu6），由六个配体配位。纳米铜簇之间存在非共价相互作用（C-H...π 和 π...π）。即使在 250 °C 时，纳米铜簇也能发光。发光强度与温度变化呈线性相关。纳米铜团簇可组装成发光纳米片，其发射可被 4-硝基苯酚淬灭。光谱分析和理论计算的结果表明，内滤光器效应和电子转移导致了上述淬灭效应。构建了一种基于发光纳米片的探针，用于测定 β-半乳糖苷酶的活性。其线性范围为 3.3-91.8 U-L-1，检测限为 0.45 U-L-1。该探针还被用于通过加标实验测定人血清中的β-半乳糖苷酶活性。回收率为 96.2% 至 101.8%。
{"title":"Temperature-dependent luminescent copper nanoclusters with noncovalent interactions for determination of β-galactosidase activity","authors":"Yanping Chang, Jingyi Wang, Hongwei Guo, Wanqing Yao, Haijiao Xie, Long Li, Xianhu Liu","doi":"10.1007/s00604-024-06844-w","DOIUrl":"10.1007/s00604-024-06844-w","url":null,"abstract":"<div><p>The synthesis of a novel bidentate ligand-protected copper nanocluster via a solid-state strategy is reported. Single-crystal X-ray diffraction analysis result reveals that the copper nanocluster features an octahedral core (Cu<sub>6</sub>) coordinated by six ligands. Noncovalent interactions (C-H<sup><b>…</b></sup>π and π<sup><b>…</b></sup>π) exist between the copper nanoclusters. The copper nanocluster displays luminescence even at 250 °C. The luminescence intensity is linearly correlated with temperature changes. The copper nanocluster can assemble into luminescent nanosheets whose emission is quenched by 4-nitrophenol. Spectroscopic analysis and theoretical calculations results demonstrate that the inner filter effect and electron transfer cause the above quenching effect. A probe based on luminescent nanosheets was constructed for β-galactosidase activity determination. The linearity range is 3.3–91.8 U·L<sup>−1</sup>, and the limit of detection is 0.45 U·L<sup>−1</sup>. This probe was also evaluated for determination of the β-galactosidase activity in human serum via spiking experiments. The recoveries ranged from 96.2% to 101.8%.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142736816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27DOI: 10.1007/s00604-024-06809-z
Ye Wang, Ming Wang, Hang You, Chao Chen, Jing Zhang, Tianhua Li, Ning Gan
A dual-mode assay was developed for screening and detecting live Escherichia coli (E. coli) and Hafnia paralvei (H. paralvei) (as two typical pathogens in aquatic environments) based on magnetic poly(phages) encoded probes (MPEP). The probes were prepared by grafting a large number of phages targeting different target bacteria on a long-chain DNA structure, respectively. They could specifically capture and enrich E. coli and H. paralvei by magnetic separation. Then, different DNA signal tags with different lengths conjugate with the corresponding MPEP-bacteria complex and form two kinds of sandwich structures, respectively. After that, the captured E. coli and H. paralvei were lysed to release both adenosine triphosphate (ATP) and DNA signal tags. The measurement includes two steps. Firstly, a portable ATP bioluminescence meter was employed to rapidly screen the positive samples that contain either of the two target bacteria. Secondly, only positive samples were injected into the microfluidic chip which could detect various DNA signal tags for accurate quantification of the target bacteria. The assay demonstrated high sensitivity (3 CFU/mL for E. coli and 5 CFU/mL for H. paralvei), high specificity (strain identification), signal amplification (20-fold), and short time (≤ 35 min). It can be applied to detect other pathogens solely by changing the relative phage in MPEP. Furthermore, the proposed dual-mode assay provides a wide prospect for rapid screening and accurate determination of live foodborne pathogens. Clinical Trial Number: nbdxms-20240322.
Graphical abstract
基于磁性多(噬菌体)编码探针(MPEP)，开发了一种用于筛选和检测活大肠杆菌（E. coli）和副嗜血杆菌（H. paralvei）（水生环境中的两种典型病原体）的双模式检测方法。这些探针是将大量针对不同目标细菌的噬菌体分别嫁接到长链 DNA 结构上制备而成的。它们可以通过磁分离特异性地捕获和富集大肠杆菌和副大肠杆菌。然后，不同长度的 DNA 信号标签与相应的 MPEP-细菌复合物共轭，分别形成两种夹层结构。然后，将捕获的大肠杆菌和帕拉维氏菌裂解，释放出三磷酸腺苷（ATP）和 DNA 信号标签。测量包括两个步骤。首先，使用便携式 ATP 生物发光仪快速筛选含有两种目标细菌中任何一种的阳性样本。其次，只将阳性样本注入微流控芯片，芯片可检测各种 DNA 信号标签，从而准确量化目标细菌。该检测方法具有灵敏度高（大肠杆菌为 3 CFU/mL，副猪嗜血杆菌为 5 CFU/mL）、特异性高（菌株鉴定）、信号放大（20 倍）和时间短（≤ 35 分钟）等特点。只需改变 MPEP 中的相对噬菌体，就可用于检测其他病原体。此外，所提出的双模式检测方法为快速筛查和准确测定活的食源性病原体提供了广阔的前景。临床试验编号：NBDXMS-20240322。
{"title":"Magnetic poly(phages) encoded probes–based dual-mode assay for rapid determination of live Escherichia coli and Hafnia paralvei based on microfluidic chip and ATP bioluminescence meter","authors":"Ye Wang, Ming Wang, Hang You, Chao Chen, Jing Zhang, Tianhua Li, Ning Gan","doi":"10.1007/s00604-024-06809-z","DOIUrl":"10.1007/s00604-024-06809-z","url":null,"abstract":"<div><p> A dual-mode assay was developed for screening and detecting live <i>Escherichia coli</i> (<i>E. coli</i>) and <i>Hafnia paralvei</i> (<i>H. paralvei</i>) (as two typical pathogens in aquatic environments) based on magnetic poly(phages) encoded probes (MPEP). The probes were prepared by grafting a large number of phages targeting different target bacteria on a long-chain DNA structure, respectively. They could specifically capture and enrich <i>E. coli</i> and <i>H. paralvei</i> by magnetic separation. Then, different DNA signal tags with different lengths conjugate with the corresponding MPEP-bacteria complex and form two kinds of sandwich structures, respectively. After that, the captured <i>E. coli</i> and <i>H. paralvei</i> were lysed to release both adenosine triphosphate (ATP) and DNA signal tags. The measurement includes two steps. Firstly, a portable ATP bioluminescence meter was employed to rapidly screen the positive samples that contain either of the two target bacteria. Secondly, only positive samples were injected into the microfluidic chip which could detect various DNA signal tags for accurate quantification of the target bacteria. The assay demonstrated high sensitivity (3 CFU/mL for <i>E. coli</i> and 5 CFU/mL for <i>H. paralvei</i>), high specificity (strain identification), signal amplification (20-fold), and short time (≤ 35 min). It can be applied to detect other pathogens solely by changing the relative phage in MPEP. Furthermore, the proposed dual-mode assay provides a wide prospect for rapid screening and accurate determination of live foodborne pathogens. Clinical Trial Number: nbdxms-20240322.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-024-06809-z.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142724594","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A photocurrent enhancing photoelectrochemical (PEC) immunosensor was developed for chloramphenicol (CAP) detection based on cation exchange reaction. The efficient split-type PEC immunosensor combined with controlled-release strategy was established using the ZnIn2S4/TiO2/Ti3C2 MXene (ZIS/T/M) composite as the photoactive material and CuO as the signal response probe. In the presence of target CAP, CuO-labeled CAP antibody (CuO-mAb) was introduced onto the microplate via a competitive-type immunoassay. Under acidic conditions, a large amount of Cu2+ released from CuO-mAb, which triggered a cation exchange reaction with the Zn2+ in ZIS/T/M-modified photoelectrode to generate CuxS, resulting in enhancing the photocurrent. As a result, the quantitative detection of CAP was achieved by detecting the photocurrent change. Under optimized conditions, the linear range of the sensor was 1 pg/mL to 50 ng/mL, and the detection limit was 0.24 pg/mL. The excellent PEC behavior of ZIS/T/M composite could be attributed to the fact that heterojunction formation improved the migration and separation of the photocarrier. Additionally, by virtue of the photocurrent-enhancing strategy via cation exchange reaction and the controlled releasing signal amplification method of ion, the PEC immunosensor has high sensitivity and satisfactory accuracy, offering great potential applications in the determination of CAP.
{"title":"Photoelectrochemical immunosensor for chloramphenicol detection based on cation exchange reaction-mediacted photocurrent enhancement of ZnIn2S4/TiO2/Ti3C2 MXene coupled with controlled-release strategy","authors":"Mengqin Zheng, Huizi Lin, Suhua Li, Shuoying Huang, Jiangwei Huang, Wenqiang Lai, Dianping Tang, Youxiu Lin","doi":"10.1007/s00604-024-06847-7","DOIUrl":"10.1007/s00604-024-06847-7","url":null,"abstract":"<div><p> A photocurrent enhancing photoelectrochemical (PEC) immunosensor was developed for chloramphenicol (CAP) detection based on cation exchange reaction. The efficient split-type PEC immunosensor combined with controlled-release strategy was established using the ZnIn<sub>2</sub>S<sub>4</sub>/TiO<sub>2</sub><b>/</b>Ti<sub>3</sub>C<sub>2</sub> MXene (ZIS/T/M) composite as the photoactive material and CuO as the signal response probe. In the presence of target CAP, CuO-labeled CAP antibody (CuO-mAb) was introduced onto the microplate via a competitive-type immunoassay. Under acidic conditions, a large amount of Cu<sup>2+</sup> released from CuO-mAb, which triggered a cation exchange reaction with the Zn<sup>2+</sup> in ZIS/T/M-modified photoelectrode to generate Cu<sub>x</sub>S, resulting in enhancing the photocurrent. As a result, the quantitative detection of CAP was achieved by detecting the photocurrent change. Under optimized conditions, the linear range of the sensor was 1 pg/mL to 50 ng/mL, and the detection limit was 0.24 pg/mL. The excellent PEC behavior of ZIS/T/M composite could be attributed to the fact that heterojunction formation improved the migration and separation of the photocarrier. Additionally, by virtue of the photocurrent-enhancing strategy via cation exchange reaction and the controlled releasing signal amplification method of ion, the PEC immunosensor has high sensitivity and satisfactory accuracy, offering great potential applications in the determination of CAP.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27DOI: 10.1007/s00604-024-06852-w
Lin Mei, Yanmei Shi, Xueke Ding, Jing Li
Surface functionalization and the combined utilization of zero‐dimensional and two‐dimensional nanomaterials is an effective method to achieve highly sensitive detection for electrochemical analysis. Using an all-in-one strategy, phthalocyanine, gold nanoparticles, and ionic liquid were successively modified on the graphene surface as a highly integrated electrode modification material. Phthalocyanine can repair the defects of reduced graphene oxide by binding to the graphene structure surface through non-covalent functionalization. The combination of ionic liquid on the surface of gold nanoparticles can enhance their physical and chemical activity while preserving their stability. The obtained phthalocyanine-coated graphene nanosheets decorated with ionic liquid-functionalized gold nanoparticles nanocomposites had enhanced electrocatalysis and conductivity ability, and were used for highly sensitive electrochemical detection of ascorbic acid in fruit juices. Excellent results were obtained for the detection of ascorbic acid in the linear range 0.05 to 50 µmol/L with a detection limit of 6.80 nmol/L (S/N = 3) and a sensitivity of 2.68 μA μM−1 cm−2, indicated that the proposed sensor strategy with multiple signal amplification can achieve higher detection sensitivity. Furthermore, the sensor was used to quantify ascorbic acid content in grapefruit and orange juice with good selectivity and accuracy. The highly integrated electroactive nanocomposites construction method described may also be used with other electrode modification materials and is anticipated to yield fresh perspectives on the advancement of ultrasensitive detection.
{"title":"Integrated tetrahydroxyphthalocyanine-coated graphene decorated with ionic liquid-functionalized gold nanoparticles for highly sensitive and selective electrochemical determination of ascorbic acid in fruit juices","authors":"Lin Mei, Yanmei Shi, Xueke Ding, Jing Li","doi":"10.1007/s00604-024-06852-w","DOIUrl":"10.1007/s00604-024-06852-w","url":null,"abstract":"<div><p>Surface functionalization and the combined utilization of zero‐dimensional and two‐dimensional nanomaterials is an effective method to achieve highly sensitive detection for electrochemical analysis. Using an all-in-one strategy, phthalocyanine, gold nanoparticles, and ionic liquid were successively modified on the graphene surface as a highly integrated electrode modification material. Phthalocyanine can repair the defects of reduced graphene oxide by binding to the graphene structure surface through non-covalent functionalization. The combination of ionic liquid on the surface of gold nanoparticles can enhance their physical and chemical activity while preserving their stability. The obtained phthalocyanine-coated graphene nanosheets decorated with ionic liquid-functionalized gold nanoparticles nanocomposites had enhanced electrocatalysis and conductivity ability, and were used for highly sensitive electrochemical detection of ascorbic acid in fruit juices. Excellent results were obtained for the detection of ascorbic acid in the linear range 0.05 to 50 µmol/L with a detection limit of 6.80 nmol/L (<i>S/N</i> = 3) and a sensitivity of 2.68 μA μM<sup>−1 </sup>cm<sup>−2</sup>, indicated that the proposed sensor strategy with multiple signal amplification can achieve higher detection sensitivity. Furthermore, the sensor was used to quantify ascorbic acid content in grapefruit and orange juice with good selectivity and accuracy. The highly integrated electroactive nanocomposites construction method described may also be used with other electrode modification materials and is anticipated to yield fresh perspectives on the advancement of ultrasensitive detection.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142737283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-11-27DOI: 10.1007/s00604-024-06842-y
Chao Zhang, Yichang Li, Hui Si, Hao Du, Lianpeng Lv, Bing Xu, Yefeng Deng, Junting Li, Hailiang Yang, Yang Zhou, Bing Wang
A point-of-care testing (POCT) platform, i.e., a portable colorimetric immunosensor based on iron oxide magnetic beads and AuNPs, has been developed for detecting leather residues. The immunosensor demonstrates a linear detection range from 1 ng/mL to 10 µg/mL, with a limit of detection (LOD) of 0.985 ng/mL. The sensor exhibits high specificity and repeatability and performs effectively in detecting leather artifacts excavated from Inner Mongolia. Thus, the proposed colorimetric immunosensor not only enables the micro-detection of leather artifacts but also shows significant potential for on-site leather detection at archaeological sites.
{"title":"A portable colorimetric immunosensor for highly sensitive point-of-care testing of leather artifacts","authors":"Chao Zhang, Yichang Li, Hui Si, Hao Du, Lianpeng Lv, Bing Xu, Yefeng Deng, Junting Li, Hailiang Yang, Yang Zhou, Bing Wang","doi":"10.1007/s00604-024-06842-y","DOIUrl":"10.1007/s00604-024-06842-y","url":null,"abstract":"<div><p> A point-of-care testing (POCT) platform, i.e., a portable colorimetric immunosensor based on iron oxide magnetic beads and AuNPs, has been developed for detecting leather residues. The immunosensor demonstrates a linear detection range from 1 ng/mL to 10 µg/mL, with a limit of detection (LOD) of 0.985 ng/mL. The sensor exhibits high specificity and repeatability and performs effectively in detecting leather artifacts excavated from Inner Mongolia. Thus, the proposed colorimetric immunosensor not only enables the micro-detection of leather artifacts but also shows significant potential for on-site leather detection at archaeological sites.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
An efficient “on-off-on” electrochemiluminescence (ECL) aptasensor utilizing dual-mechanism quenching was constructed for detecting furanyl fentanyl (FuF). The first signal “on” state was achieved by novel dual-ligand zinc metal-organic frameworks (Zn-MOFs), which were synthesized by self-assembly reaction using zinc atom clusters as metal nodes, achieving strong and stable ECL emission. The “off” state was realized by the energy and electron quenching effect of copper-doped WO3. Specifically, in addition to the overlap of the UV-Vis spectrum, energy transfer existed between the acceptor and donor after doping copper. Therefore, copper created a “highway” for electron transfer between the donor and acceptor, which greatly improved the quenching efficiency. Simultaneously, employing multivalent aptamers as capture probes augmented the binding affinity and probability of association between aptamer and target through a synergistic multivalent effect. Consequently, the second “on” state was the ECL signal restored by introducing FuF. Benefiting from the combination of the multivalent aptamers strategy with the “on-off-on” design, the developed aptasensor showed excellent linearity (1.0 × 10−13 to 1.0 × 10−6 g/L) with a low limit of detection of 5.5 × 10−14 g/L (S/N = 3). Additionally, it demonstrates a relative standard deviation (RSD) of less than 5% and good recovery ranging from 97.6 to 102%. The proposed aptasensor presents considerable potential for rapid, sensitive, and accurate determination of FuF.
{"title":"Ultrasensitive electrochemiluminescencent multivalent aptamer sensor based on energy and electron transfer dual quenching tactics","authors":"Yuan Ni, Ding Jiang, Xiaomei An, Wenchang Wang, Zheng Sun, Haibo Li, Hiroshi Shiigi, Zhidong Chen","doi":"10.1007/s00604-024-06833-z","DOIUrl":"10.1007/s00604-024-06833-z","url":null,"abstract":"<div><p> An efficient “on-off-on” electrochemiluminescence (ECL) aptasensor utilizing dual-mechanism quenching was constructed for detecting furanyl fentanyl (FuF). The first signal “on” state was achieved by novel dual-ligand zinc metal-organic frameworks (Zn-MOFs), which were synthesized by self-assembly reaction using zinc atom clusters as metal nodes, achieving strong and stable ECL emission. The “off” state was realized by the energy and electron quenching effect of copper-doped WO<sub>3</sub>. Specifically, in addition to the overlap of the UV-Vis spectrum, energy transfer existed between the acceptor and donor after doping copper. Therefore, copper created a “highway” for electron transfer between the donor and acceptor, which greatly improved the quenching efficiency. Simultaneously, employing multivalent aptamers as capture probes augmented the binding affinity and probability of association between aptamer and target through a synergistic multivalent effect. Consequently, the second “on” state was the ECL signal restored by introducing FuF. Benefiting from the combination of the multivalent aptamers strategy with the “on-off-on” design, the developed aptasensor showed excellent linearity (1.0 × 10<sup>−13</sup> to 1.0 × 10<sup>−6</sup> g/L) with a low limit of detection of 5.5 × 10<sup>−14</sup> g/L (<i>S</i>/<i>N</i> = 3). Additionally, it demonstrates a relative standard deviation (RSD) of less than 5% and good recovery ranging from 97.6 to 102%. The proposed aptasensor presents considerable potential for rapid, sensitive, and accurate determination of FuF.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"191 12","pages":""},"PeriodicalIF":5.3,"publicationDate":"2024-11-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142714543","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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