Pub Date : 2024-08-12DOI: 10.1007/s00604-024-06607-7
Xiangzhen Chen, Jun Xu, Yongxin Li, Yuanyuan Huang, Lina Zhang, Ning Bi, Jian Gou, Tongqian Zhao, Lei Jia
Tetracycline (TC) has been widely used in clinical medicine and animal growth promotion due to its broad-spectrum antibacterial properties and affordable prices. Unfortunately, the high toxicity and difficult degradation rate of TC molecules make them easy to accumulate in the environment, which breaks the ecological balance and seriously threatens human health. Rapid and accurate detection of TC residue levels is important for ensuring water quality and food safety. Recently, fluorescence detection technology of TC residues has developed rapidly. Lanthanide nanomaterials, based on the high luminescence properties of lanthanide ions and the high matching with TC energy levels, are favored in the real-time trace detection of TC due to their advantages of high sensitivity, rapidity, and high selectivity. Therefore, they are considered potential substitutes for traditional detection methods. This review summarizes the synthesis strategy, TC response mechanism, removal mechanism, and applications in intelligent sensing. Finally, the development of lanthanide nanomaterials for TC fluorescence detection and removal is reasonably summarized and prospected. This review provides a reference for the establishment of a method for the accurate determination of TC content in complex food matrices.
{"title":"Recent progress in lanthanide-based fluorescent nanomaterials for tetracycline detection and removal.","authors":"Xiangzhen Chen, Jun Xu, Yongxin Li, Yuanyuan Huang, Lina Zhang, Ning Bi, Jian Gou, Tongqian Zhao, Lei Jia","doi":"10.1007/s00604-024-06607-7","DOIUrl":"10.1007/s00604-024-06607-7","url":null,"abstract":"<p><p>Tetracycline (TC) has been widely used in clinical medicine and animal growth promotion due to its broad-spectrum antibacterial properties and affordable prices. Unfortunately, the high toxicity and difficult degradation rate of TC molecules make them easy to accumulate in the environment, which breaks the ecological balance and seriously threatens human health. Rapid and accurate detection of TC residue levels is important for ensuring water quality and food safety. Recently, fluorescence detection technology of TC residues has developed rapidly. Lanthanide nanomaterials, based on the high luminescence properties of lanthanide ions and the high matching with TC energy levels, are favored in the real-time trace detection of TC due to their advantages of high sensitivity, rapidity, and high selectivity. Therefore, they are considered potential substitutes for traditional detection methods. This review summarizes the synthesis strategy, TC response mechanism, removal mechanism, and applications in intelligent sensing. Finally, the development of lanthanide nanomaterials for TC fluorescence detection and removal is reasonably summarized and prospected. This review provides a reference for the establishment of a method for the accurate determination of TC content in complex food matrices.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141970344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-11DOI: 10.1007/s00604-024-06611-x
Li Ruiyi, Wei Mengyu, Zhou Xinyi, Li Zaijun, Liu Xiaohao
The synthesis of cobalt nanocrystal-graphene quantum dot-Ti3C2TX monolithic film electrode (Co-GQD-Ti3C2TX) is reported via self-assembly of Ti3C2TX nanosheets induced by protonated arginine-functionalized graphene quantum dot and subsequent reduction of cobalt (III). The resulting Co-GQD-Ti3C2TX shows good monolithic architecture, mechanical property, dispersibility and conductivity. The structure achieves excellent supercapacitor and sensing behavior. The self-charging supercapacitor produced by printing viscous Co-GQD-Ti3C2TX hydrogel on the back of flexible solar cell surface provides high specific capacitance (296 F g-1 at 1 A g-1), high-rate capacity (153 F g-1 at 20 A g-1), capacity retention (98.1% over 10,000-cycle) and energy density (29.6 W h kg-1 at 299.9 W kg-1). The electrochemical chip produced by printing Co-GQD-Ti3C2TX hydrogel on paper exhibits sensitive electrochemical response towards uric acid. The increase of uric acid between 0.01 and 800 μM causes a linear increase in differential pulse voltammetry signal with a detection limit of 0.0032 μM. The self-powered sensing platform integrating self-charging supercapacitor, electrochemical chip and micro electrochemical workstation was contentedly applied to monitoring uric acid in sweats and shows one broad application prospect in wearable electronic health monitoring device.
报告通过质子化精氨酸功能化石墨烯量子点诱导 Ti3C2TX 纳米片的自组装以及随后的钴(III)还原,合成了钴纳米晶体-石墨烯量子点-Ti3C2TX 单片电极(Co-GQD-Ti3C2TX)。由此产生的 Co-GQD-Ti3C2TX 显示出良好的整体结构、机械性能、分散性和导电性。该结构实现了优异的超级电容器和传感性能。通过在柔性太阳能电池表面背面印刷粘性 Co-GQD-Ti3C2TX 水凝胶制作的自充电超级电容器具有高比电容(1 A g-1 时为 296 F g-1)、高倍率容量(20 A g-1 时为 153 F g-1)、容量保持率(10,000 周期以上为 98.1%)和能量密度(299.9 W kg-1 时为 29.6 W h kg-1)。通过在纸上印刷 Co-GQD-Ti3C2TX 水凝胶制作的电化学芯片对尿酸具有灵敏的电化学响应。尿酸在 0.01 至 800 μM 之间的增加会导致差分脉冲伏安法信号的线性增加,检测限为 0.0032 μM。集自充电超级电容器、电化学芯片和微型电化学工作站于一体的自供电传感平台成功应用于监测汗液中的尿酸,在可穿戴电子健康监测设备中展现了广阔的应用前景。
{"title":"Self-powered sensing platform for monitoring uric acid in sweat using cobalt nanocrystal-graphene quantum dot-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> monolithic film electrode with excellent supercapacitor and sensing behavior.","authors":"Li Ruiyi, Wei Mengyu, Zhou Xinyi, Li Zaijun, Liu Xiaohao","doi":"10.1007/s00604-024-06611-x","DOIUrl":"10.1007/s00604-024-06611-x","url":null,"abstract":"<p><p>The synthesis of cobalt nanocrystal-graphene quantum dot-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> monolithic film electrode (Co-GQD-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub>) is reported via self-assembly of Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> nanosheets induced by protonated arginine-functionalized graphene quantum dot and subsequent reduction of cobalt (III). The resulting Co-GQD-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> shows good monolithic architecture, mechanical property, dispersibility and conductivity. The structure achieves excellent supercapacitor and sensing behavior. The self-charging supercapacitor produced by printing viscous Co-GQD-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> hydrogel on the back of flexible solar cell surface provides high specific capacitance (296 F g<sup>-1</sup> at 1 A g<sup>-1</sup>), high-rate capacity (153 F g<sup>-1</sup> at 20 A g<sup>-1</sup>), capacity retention (98.1% over 10,000-cycle) and energy density (29.6 W h kg<sup>-1</sup> at 299.9 W kg<sup>-1</sup>). The electrochemical chip produced by printing Co-GQD-Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> hydrogel on paper exhibits sensitive electrochemical response towards uric acid. The increase of uric acid between 0.01 and 800 μM causes a linear increase in differential pulse voltammetry signal with a detection limit of 0.0032 μM. The self-powered sensing platform integrating self-charging supercapacitor, electrochemical chip and micro electrochemical workstation was contentedly applied to monitoring uric acid in sweats and shows one broad application prospect in wearable electronic health monitoring device.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A ratiometric fluorescence probe based on carbon quantum dots with 420 nm emission (bCQDs) and a p-phenylenediamine-derived fluorescence probe with 550 nm emission (yprobe) is constructed for the detection of Mn2+. The presence of Mn2+ results in the enhanced absorption band at 400 nm of yprobe, and the fluorescence of yprobe is significantly enhanced based on the chelation-enhanced fluorescence mechanism. The fluorescence of bCQDs is then quenched based on the inner filtration effect. The ratio (I550/I420) linearly increases with the increase of Mn2+ concentration within 2.00 × 10-7-1.50 × 10-6 M, and the limit of detection is 1.76 × 10-9 M. Given the fluorescence color changing from blue to yellow, the visual sensing of Mn2+ is feasible based on bCQDs/yprobe coupled with RGB value analysis. The practicability of the proposed method has been verified in tap water, lake water, and sparkling water beverage, indicating that bCQDs/yprobe has promising application in Mn2+ monitoring.
{"title":"Ratiometric fluorescence probe based on carbon dots and p-phenylenediamine-derived nanoparticles for the sensitive detection of manganese ions.","authors":"Anqi Hu, Guoqing Chen, Anlan Huang, Lei Li, Chaoqun Ma, Taiqun Yang, Hui Gao, Jiao Gu, Chun Zhu, Yunpeng Shang, Yamin Wu","doi":"10.1007/s00604-024-06612-w","DOIUrl":"https://doi.org/10.1007/s00604-024-06612-w","url":null,"abstract":"<p><p>A ratiometric fluorescence probe based on carbon quantum dots with 420 nm emission (bCQDs) and a p-phenylenediamine-derived fluorescence probe with 550 nm emission (yprobe) is constructed for the detection of Mn<sup>2+</sup>. The presence of Mn<sup>2+</sup> results in the enhanced absorption band at 400 nm of yprobe, and the fluorescence of yprobe is significantly enhanced based on the chelation-enhanced fluorescence mechanism. The fluorescence of bCQDs is then quenched based on the inner filtration effect. The ratio (I<sub>550</sub>/I<sub>420</sub>) linearly increases with the increase of Mn<sup>2+</sup> concentration within 2.00 × 10<sup>-7</sup>-1.50 × 10<sup>-6</sup> M, and the limit of detection is 1.76 × 10<sup>-9</sup> M. Given the fluorescence color changing from blue to yellow, the visual sensing of Mn<sup>2+</sup> is feasible based on bCQDs/yprobe coupled with RGB value analysis. The practicability of the proposed method has been verified in tap water, lake water, and sparkling water beverage, indicating that bCQDs/yprobe has promising application in Mn<sup>2+</sup> monitoring.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141911312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-09DOI: 10.1007/s00604-024-06558-z
Yanping Wang, Min Guo, Yuanyuan Li, Yan Chen, Hong Wei, Xiaohui Mo, Guolin Chai, Yongling Du, Fangdi Hu
A dual-template molecularly imprinted electrochemical sensor was developed for the simultaneous detection of serotonin (5-HT) and glutamate (Glu). First, amino-functionalized reduced graphene oxide (NRGO) was used as the modification material of a GCE to increase its electrical conductivity and specific surface area, using Glu and 5-HT as dual-template molecules and o-phenylenediamine (OPD) with self-polymerization ability as functional monomers. Through self-assembly and electropolymerization, dual-template molecularly imprinted polymers were formed on the electrode. After removing the templates, the specific recognition binding sites were exposed. The amount of NRGO, polymerization parameters, and elution parameters were further optimized to construct a dual-template molecularly imprinted electrochemical sensor, which can specifically recognize double-target molecules Glu and 5-HT. The differential pulse voltammetry (DPV) technique was used to achieve simultaneous detection of Glu and 5-HT based on their distinct electrochemical activities under specific conditions. The sensor showed a good linear relationship for Glu and 5-HT in the range 1 ~ 100 μM, and the detection limits were 0.067 μM and 0.047 μM (S/N = 3), respectively. The sensor has good reproducibility, repeatability, and selectivity. It was successfully utilized to simultaneously detect Glu and 5-HT in mouse serum, offering a more dependable foundation for objectively diagnosing and early warning of depression. Additionally, the double signal sensing strategy also provides a new approach for the simultaneous detection of both electroactive and non-electroactive substances.
{"title":"Development of a dual-template molecularly imprinted electrochemical sensor for the simultaneous detection of depression markers 5-HT and Glu.","authors":"Yanping Wang, Min Guo, Yuanyuan Li, Yan Chen, Hong Wei, Xiaohui Mo, Guolin Chai, Yongling Du, Fangdi Hu","doi":"10.1007/s00604-024-06558-z","DOIUrl":"10.1007/s00604-024-06558-z","url":null,"abstract":"<p><p>A dual-template molecularly imprinted electrochemical sensor was developed for the simultaneous detection of serotonin (5-HT) and glutamate (Glu). First, amino-functionalized reduced graphene oxide (NRGO) was used as the modification material of a GCE to increase its electrical conductivity and specific surface area, using Glu and 5-HT as dual-template molecules and o-phenylenediamine (OPD) with self-polymerization ability as functional monomers. Through self-assembly and electropolymerization, dual-template molecularly imprinted polymers were formed on the electrode. After removing the templates, the specific recognition binding sites were exposed. The amount of NRGO, polymerization parameters, and elution parameters were further optimized to construct a dual-template molecularly imprinted electrochemical sensor, which can specifically recognize double-target molecules Glu and 5-HT. The differential pulse voltammetry (DPV) technique was used to achieve simultaneous detection of Glu and 5-HT based on their distinct electrochemical activities under specific conditions. The sensor showed a good linear relationship for Glu and 5-HT in the range 1 ~ 100 μM, and the detection limits were 0.067 μM and 0.047 μM (S/N = 3), respectively. The sensor has good reproducibility, repeatability, and selectivity. It was successfully utilized to simultaneously detect Glu and 5-HT in mouse serum, offering a more dependable foundation for objectively diagnosing and early warning of depression. Additionally, the double signal sensing strategy also provides a new approach for the simultaneous detection of both electroactive and non-electroactive substances.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-09DOI: 10.1007/s00604-024-06593-w
Chao Li, Yangmin Ma, Cheng Fan, Chong He, Siyue Ma
A dual-emission ratiometric fluorescence sensor (CDs@CdTe@MIP) with a self-calibration function was successfully constructed for AMO detection. In the CDs@CdTe@MIP system, non-imprinted polymer-coated CDs and molecule-imprinted polymer-coated CdTe quantum dots were used as the reference signal and response elements, respectively. The added AMO quenched the fluorescence of the CdTe quantum dots, whereas the fluorescence intensity of the CDs remained almost unchanged. The AMO concentration was monitored using the fluorescence intensity ratio (log(I647/I465)0/(I647/I465)) to reduce interference from the testing environment. The sensor with a low detection limit of 0.15 μg/L enabled detection of the AMO concentration within 6 min. The ratiometric fluorescence sensor was used to detect AMO in spiked pork samples; it exhibited a high recovery efficiency and relative standard deviation (RSD) of 97.94-103.70% and 3.77-4.37%, respectively. The proposed highly sensitive and selective platform opens avenues for sensitive, reliable, and rapid determination of pharmaceuticals in the environment and food safety monitoring using ratiometric sensors.
成功构建了一种具有自校准功能的双发射比率荧光传感器(CDs@CdTe@MIP),用于检测 AMO。在 CDs@CdTe@MIP 系统中,非压印聚合物涂层 CD 和分子压印聚合物涂层碲化镉量子点分别用作参考信号和响应元件。添加的 AMO 可淬灭碲镉(CdTe)量子点的荧光,而 CD 的荧光强度几乎保持不变。利用荧光强度比(log(I647/I465)0/(I647/I465))来监测 AMO 的浓度,以减少测试环境的干扰。传感器的检测限低至 0.15 微克/升,可在 6 分钟内检测到 AMO 浓度。该比率荧光传感器用于检测猪肉样品中的 AMO,其回收率和相对标准偏差(RSD)分别为 97.94-103.70% 和 3.77-4.37%。所提出的高灵敏度和高选择性平台为利用比率计量传感器灵敏、可靠、快速地测定环境中的药物和监测食品安全开辟了道路。
{"title":"Highly sensitive and selective detection of amoxicillin using molecularly imprinted ratiometric fluorescent nanosensor based on quantum dots.","authors":"Chao Li, Yangmin Ma, Cheng Fan, Chong He, Siyue Ma","doi":"10.1007/s00604-024-06593-w","DOIUrl":"10.1007/s00604-024-06593-w","url":null,"abstract":"<p><p>A dual-emission ratiometric fluorescence sensor (CDs@CdTe@MIP) with a self-calibration function was successfully constructed for AMO detection. In the CDs@CdTe@MIP system, non-imprinted polymer-coated CDs and molecule-imprinted polymer-coated CdTe quantum dots were used as the reference signal and response elements, respectively. The added AMO quenched the fluorescence of the CdTe quantum dots, whereas the fluorescence intensity of the CDs remained almost unchanged. The AMO concentration was monitored using the fluorescence intensity ratio (log(I<sub>647</sub>/I<sub>465</sub>)<sub>0</sub>/(I<sub>647</sub>/I<sub>465</sub>)) to reduce interference from the testing environment. The sensor with a low detection limit of 0.15 μg/L enabled detection of the AMO concentration within 6 min. The ratiometric fluorescence sensor was used to detect AMO in spiked pork samples; it exhibited a high recovery efficiency and relative standard deviation (RSD) of 97.94-103.70% and 3.77-4.37%, respectively. The proposed highly sensitive and selective platform opens avenues for sensitive, reliable, and rapid determination of pharmaceuticals in the environment and food safety monitoring using ratiometric sensors.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905433","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-09DOI: 10.1007/s00604-024-06602-y
Juan José García-Guzmán, José Manuel Jiménez Heras, David López-Iglesias, Rafael Jesús González-Álvarez, Laura Cubillana-Aguilera, Carmen González Macías, Juan Jesús Fernández Alba, José María Palacios-Santander
A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.
我们开发了一种基于 LOx 的电化学生物传感器,用于测定高浓度乳酸盐。在构建生物传感器时,采用旋涂工艺将壳聚糖层和 Nafion 层整合在一起,与滴注工艺相比,表面孔隙更少。在批处理和流动模式下,对所产生的用于测定乳酸盐的生物传感器的分析性能进行了评估,在 0.5 至 20 mM 的浓度范围内,两种模式都显示出令人满意的结果,可用于评估乳酸酸中毒。最后,使用开发的生物传感器估算了原始血清样本中的乳酸水平,并用血气分析仪进行了验证。根据这些结果,开发的生物传感器在适当微型化后有望用于医疗环境。此外,还开发了一种基于普通聚苯胺电化学传感器的 pH 探针,用于辅助生物传感器监测乳酸酸中毒,在 6.0 至 8.0 mM 的储备溶液和原始血浆样本中均取得了出色的结果。使用血气分析仪和 pH 计这两种不同的方法对结果进行了确认。因此,使用这两种(生物)传感器可以在连续流条件下实现乳酸酸中毒监测。
{"title":"New spin coated multilayer lactate biosensor for acidosis monitoring in continuous flow assisted with an electrochemical pH probe.","authors":"Juan José García-Guzmán, José Manuel Jiménez Heras, David López-Iglesias, Rafael Jesús González-Álvarez, Laura Cubillana-Aguilera, Carmen González Macías, Juan Jesús Fernández Alba, José María Palacios-Santander","doi":"10.1007/s00604-024-06602-y","DOIUrl":"10.1007/s00604-024-06602-y","url":null,"abstract":"<p><p>A LOx-based electrochemical biosensor for high-level lactate determination was developed. For the construction of the biosensor, chitosan and Nafion layers were integrated by using a spin coating procedure, leading to less porous surfaces in comparison with those recorded after a drop casting procedure. The analytical performance of the resulting biosensor for lactate determination was evaluated in batch and flow regime, displaying satisfactory results in both modes ranging from 0.5 to 20 mM concentration range for assessing the lactic acidosis. Finally, the lactate levels in raw serum samples were estimated using the biosensor developed and verified with a blood gas analyzer. Based on these results, the biosensor developed is promising for its use in healthcare environment, after its proper miniaturization. A pH probe based on common polyaniline-based electrochemical sensor was also developed to assist the biosensor for the lactic acidosis monitoring, leading to excellent results in stock solutions ranging from 6.0 to 8.0 mM and raw plasma samples. The results were confirmed by using two different approaches, blood gas analyzer and pH-meter. Consequently, the lactic acidosis monitoring could be achieved in continuous flow regime using both (bio)sensors.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11315777/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A sophisticated electrochemical sensor is presented employing a glassy carbon electrode (GCE) modified with a novel composite of synthesized graphitic carbon nitride (g-C3N4) and CoNiO2 bimetallic oxide nanoparticles (g-C3N4/CoNiO2). The sensor's electrocatalytic capabilities for Sunitinib (SUNI) oxidation were demonstrated exceptional performance with a calculated detection limit (LOD) of 52.0 nM. The successful synthesis and integrity of the composite were confirmed through meticulous characterization using various techniques. FT-IR analysis affirmed the successful synthesis of g-C3N4/CoNiO2 by providing insights into its molecular structure. XRD, FE-SEM, SEM-EDX, and BET analyses collectively validated the material's structural integrity, surface morphology, and electrocatalytic performance. Optimization of key analytical parameters, such as loading volume, concentration, electrolyte solution type, and pH, enhanced the electrocatalytic sensing capabilities of g-C3N4/CoNiO2. The synergistic interaction between g-C3N4 and CoNiO2 bimetallic oxide nanoparticles executed the sensor highly effective in the electrical oxidation of SUNI. Across a concentration range of 0.1-83.8 µM SUNI, the anodic peak current exhibited a linear increase with good precision. Application of the newly developed g-C3N4/CoNiO2 system to detect SUNI in a variety of samples, including urine, human serum, and capsule dosage forms, obtained satisfactory recoveries ranging from 97.1 to 103.0%. This methodology offers a novel approach to underscore the potential of the developed sensor for applications in biological and pharmaceutical monitoring.
{"title":"Glassy carbon electrodes modified with graphitic carbon nitride nanosheets and CoNiO<sub>2</sub> bimetallic oxide nanoparticles as electrochemical sensor for Sunitinib detection in human fluid matrices and pharmaceutical samples.","authors":"Ghazaleh Kholafazadehastamal, Nevin Erk, Asena Ayse Genc, Zeliha Erbas, Mustafa Soylak","doi":"10.1007/s00604-024-06605-9","DOIUrl":"10.1007/s00604-024-06605-9","url":null,"abstract":"<p><p>A sophisticated electrochemical sensor is presented employing a glassy carbon electrode (GCE) modified with a novel composite of synthesized graphitic carbon nitride (g-C<sub>3</sub>N<sub>4</sub>) and CoNiO<sub>2</sub> bimetallic oxide nanoparticles (g-C<sub>3</sub>N<sub>4</sub>/CoNiO<sub>2</sub>). The sensor's electrocatalytic capabilities for Sunitinib (SUNI) oxidation were demonstrated exceptional performance with a calculated detection limit (LOD) of 52.0 nM. The successful synthesis and integrity of the composite were confirmed through meticulous characterization using various techniques. FT-IR analysis affirmed the successful synthesis of g-C<sub>3</sub>N<sub>4</sub>/CoNiO<sub>2</sub> by providing insights into its molecular structure. XRD, FE-SEM, SEM-EDX, and BET analyses collectively validated the material's structural integrity, surface morphology, and electrocatalytic performance. Optimization of key analytical parameters, such as loading volume, concentration, electrolyte solution type, and pH, enhanced the electrocatalytic sensing capabilities of g-C<sub>3</sub>N<sub>4</sub>/CoNiO<sub>2</sub>. The synergistic interaction between g-C<sub>3</sub>N<sub>4</sub> and CoNiO<sub>2</sub> bimetallic oxide nanoparticles executed the sensor highly effective in the electrical oxidation of SUNI. Across a concentration range of 0.1-83.8 µM SUNI, the anodic peak current exhibited a linear increase with good precision. Application of the newly developed g-C<sub>3</sub>N<sub>4</sub>/CoNiO<sub>2</sub> system to detect SUNI in a variety of samples, including urine, human serum, and capsule dosage forms, obtained satisfactory recoveries ranging from 97.1 to 103.0%. This methodology offers a novel approach to underscore the potential of the developed sensor for applications in biological and pharmaceutical monitoring.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141905432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-07DOI: 10.1007/s00604-024-06594-9
Xue Wei, Xiaoxiao Zhao, Dandan Sui, Xu Chen, Wensheng Yang
An antifouling peptide hydrogel-based electrochemical biosensor was developed for real-time monitoring of hydrogen peroxide (H2O2) and nitric oxide (NO) released by 3D cultured breast cancer cells upon drug stimulation. Platinum nanoparticles (Pt NPs) were electrodeposited on titanium mesh (Pt NPs/TM) to enhance sensitivity and shown to possess excellent electrocatalytic ability toward H2O2 and NO. The composite hydrogel formed by co-assembling of N-fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF) and a fluorine methoxycarbonyl group-functionalized Lys-(Fmoc)-Asp was coated on Pt NPs/TM electrode surface to provide cellular scaffolding. Their favorable biocompatibility promoted cell adhesion and growth, while good hydrophilicity endowed the sensor with greatly enhanced antifouling capability in complex cell culture environments. The biosensor successfully determined H2O2 and NO secretion from both non-metastatic and metastatic breast cancer cells in real time. Our results demonstrated robust associations between reactive oxygen species (ROS) and reactive nitrogen species (RNS) production and cell malignancy, with the main difference in oxidative stress between the two subtypes of cells being NO release, particularly emphasizing RNS's critical leading in driving cancer metastasis and invasion progression. This sensor holds great potential for cell-release research under the in vivo-like microenvironment and could reveal RNS as an attractive therapeutic target for treating breast cancer.
研究人员开发了一种基于防污肽水凝胶的电化学生物传感器,用于实时监测三维培养的乳腺癌细胞在药物刺激下释放的过氧化氢(H2O2)和一氧化氮(NO)。铂纳米粒子(Pt NPs)被电沉积在钛网(Pt NPs/TM)上以提高灵敏度,并被证明对 H2O2 和 NO 具有优异的电催化能力。由 N-芴甲氧羰基二苯丙氨酸(Fmoc-FF)和氟甲氧羰基官能化 Lys-(Fmoc)-Asp 共同组装形成的复合水凝胶被涂覆在 Pt NPs/TM 电极表面,为细胞提供支架。它们良好的生物相容性促进了细胞的粘附和生长,而良好的亲水性则大大增强了传感器在复杂细胞培养环境中的防污能力。该生物传感器成功地实时测定了非转移性和转移性乳腺癌细胞的 H2O2 和 NO 分泌。我们的研究结果表明,活性氧(ROS)和活性氮(RNS)的产生与细胞的恶性程度密切相关,两种亚型细胞氧化应激的主要区别在于 NO 的释放,这特别强调了 RNS 在推动癌症转移和侵袭进程中的关键作用。该传感器在类活体微环境下的细胞释放研究方面具有巨大潜力,并可能揭示 RNS 是治疗乳腺癌的一个有吸引力的治疗靶点。
{"title":"Peptide hydrogel based electrochemical biosensor for simultaneous monitoring of H<sub>2</sub>O<sub>2</sub> and NO released from three-dimensional cultured breast cancer cells.","authors":"Xue Wei, Xiaoxiao Zhao, Dandan Sui, Xu Chen, Wensheng Yang","doi":"10.1007/s00604-024-06594-9","DOIUrl":"10.1007/s00604-024-06594-9","url":null,"abstract":"<p><p>An antifouling peptide hydrogel-based electrochemical biosensor was developed for real-time monitoring of hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) and nitric oxide (NO) released by 3D cultured breast cancer cells upon drug stimulation. Platinum nanoparticles (Pt NPs) were electrodeposited on titanium mesh (Pt NPs/TM) to enhance sensitivity and shown to possess excellent electrocatalytic ability toward H<sub>2</sub>O<sub>2</sub> and NO. The composite hydrogel formed by co-assembling of N-fluorenylmethoxycarbonyl diphenylalanine (Fmoc-FF) and a fluorine methoxycarbonyl group-functionalized Lys-(Fmoc)-Asp was coated on Pt NPs/TM electrode surface to provide cellular scaffolding. Their favorable biocompatibility promoted cell adhesion and growth, while good hydrophilicity endowed the sensor with greatly enhanced antifouling capability in complex cell culture environments. The biosensor successfully determined H<sub>2</sub>O<sub>2</sub> and NO secretion from both non-metastatic and metastatic breast cancer cells in real time. Our results demonstrated robust associations between reactive oxygen species (ROS) and reactive nitrogen species (RNS) production and cell malignancy, with the main difference in oxidative stress between the two subtypes of cells being NO release, particularly emphasizing RNS's critical leading in driving cancer metastasis and invasion progression. This sensor holds great potential for cell-release research under the in vivo-like microenvironment and could reveal RNS as an attractive therapeutic target for treating breast cancer.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-07DOI: 10.1007/s00604-024-06603-x
Shendong Liu, Xinlian Chen, Hong Zhao, Tianran Lin, Li Hou, Shulin Zhao
An ultrasensitive photothermal assay was designed for point-of-care testing (POCT) of tumor markers based on a filter membrane. Firstly, Cu2-xSe was successfully encapsulated in liposome spheres with biotin on the surface and connected to carcinoembryonic antigen (CEA) aptamer with 3'end modified biotin by streptavidin. Secondly, the CEA antibody was successfully modified on the surface of the nitrocellulose membrane through simple incubation. Finally, the assay process was completed using a disposable syringe, and the temperature was recorded using a handheld infrared temperature detector. In the range 0-50 ng mL-1, the temperature change of the nitrocellulose membrane has a strong linear relationship with CEA concentration, and the detection limit is 0.097 ng mL-1. It is worth noting that the entire testing process can be easily performed in 10 min, much shorter than traditional clinical methods. In addition, this method was successfully applied to the quantitative determination of CEA levels in human serum samples with a recovery of 96.2-103.3%. This rapid assay can be performed by "one suction and one push" through a disposable syringe, which is simple to operate, and the excellent sensitivity reveals the great potential of the proposed strategy in the POCT of tumor biomarkers.
研究人员设计了一种基于滤膜的超灵敏光热检测方法,用于肿瘤标志物的床旁检测(POCT)。首先,Cu2-xSe 被成功封装在表面带有生物素的脂质体球中,并通过链霉亲和素与带有 3'end 修饰生物素的癌胚抗原(CEA)适配体连接。其次,通过简单的孵育,成功地将 CEA 抗体修饰在硝酸纤维素膜表面。最后,使用一次性注射器完成检测过程,并使用手持式红外温度检测器记录温度。在 0-50 ng mL-1 的范围内,硝酸纤维素膜的温度变化与 CEA 浓度有很强的线性关系,检测限为 0.097 ng mL-1。值得一提的是,整个检测过程可在 10 分钟内轻松完成,比传统的临床方法短得多。此外,该方法还成功应用于人体血清样本中 CEA 水平的定量检测,回收率高达 96.2-103.3%。这种快速检测方法只需通过一次性注射器 "一吸一推 "即可完成,操作简单,而且灵敏度极高,揭示了所提出的策略在肿瘤生物标志物 POCT 中的巨大潜力。
{"title":"Rapid photothermal assay for ultrasensitive point-of-care detection of tumor markers based on a filter membrane.","authors":"Shendong Liu, Xinlian Chen, Hong Zhao, Tianran Lin, Li Hou, Shulin Zhao","doi":"10.1007/s00604-024-06603-x","DOIUrl":"10.1007/s00604-024-06603-x","url":null,"abstract":"<p><p>An ultrasensitive photothermal assay was designed for point-of-care testing (POCT) of tumor markers based on a filter membrane. Firstly, Cu<sub>2-x</sub>Se was successfully encapsulated in liposome spheres with biotin on the surface and connected to carcinoembryonic antigen (CEA) aptamer with 3'end modified biotin by streptavidin. Secondly, the CEA antibody was successfully modified on the surface of the nitrocellulose membrane through simple incubation. Finally, the assay process was completed using a disposable syringe, and the temperature was recorded using a handheld infrared temperature detector. In the range 0-50 ng mL<sup>-1</sup>, the temperature change of the nitrocellulose membrane has a strong linear relationship with CEA concentration, and the detection limit is 0.097 ng mL<sup>-1</sup>. It is worth noting that the entire testing process can be easily performed in 10 min, much shorter than traditional clinical methods. In addition, this method was successfully applied to the quantitative determination of CEA levels in human serum samples with a recovery of 96.2-103.3%. This rapid assay can be performed by \"one suction and one push\" through a disposable syringe, which is simple to operate, and the excellent sensitivity reveals the great potential of the proposed strategy in the POCT of tumor biomarkers.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141900554","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-08-07DOI: 10.1007/s00604-024-06585-w
Yang Chen, Zixin Yang, Jiaqian Qi, Funan Chen
MoO3-x NPs was rapidly synthesized at room temperature by an easy stirring method. It was interesting to find that MoO3-x NPs induce OH- to generate active free radicals (ROS), which is a highly promising property in chemiluminescence (CL). Benefiting from the abundant oxygen vacancy, MoO3-x NPs adsorbs H2O2 and turn it into ·OH. The oxidase activity of fluorescein under visible light had already been reported, which catalyzes dissolved oxygen to become O2-· and continue to convert to H2O2. By creating the synergy effect with fluorescein, MoO3-x NPs strengthen the CL intensity of K3[Fe(CN)6]-fluorescein system significantly. Utilizing the quench effect of uric acid for the CL intensity, we developed a rapid, simple, and highly sensitive CL platform for uric acid detection. The linear range was 5-80 µM and the detection limit (LOD) for uric acid was 3.11 µM (S/N = 3). This work expanded the application of MoO3-x NPs in the CL field and developed a simple and highly sensitive CL sensing system to detect UA in human saliva.
{"title":"Synergistic enhancement of fluorescein-K<sub>3</sub>[Fe(CN)<sub>6</sub>] CL by MoO<sub>3-x</sub> NPs for sensitive and noninvasive detection of uric acid in saliva.","authors":"Yang Chen, Zixin Yang, Jiaqian Qi, Funan Chen","doi":"10.1007/s00604-024-06585-w","DOIUrl":"https://doi.org/10.1007/s00604-024-06585-w","url":null,"abstract":"<p><p>MoO<sub>3-x</sub> NPs was rapidly synthesized at room temperature by an easy stirring method. It was interesting to find that MoO<sub>3-x</sub> NPs induce OH<sup>-</sup> to generate active free radicals (ROS), which is a highly promising property in chemiluminescence (CL). Benefiting from the abundant oxygen vacancy, MoO<sub>3-x</sub> NPs adsorbs H<sub>2</sub>O<sub>2</sub> and turn it into ·OH. The oxidase activity of fluorescein under visible light had already been reported, which catalyzes dissolved oxygen to become O<sub>2</sub><sup>-</sup>· and continue to convert to H<sub>2</sub>O<sub>2</sub>. By creating the synergy effect with fluorescein, MoO<sub>3-x</sub> NPs strengthen the CL intensity of K<sub>3</sub>[Fe(CN)<sub>6</sub>]-fluorescein system significantly. Utilizing the quench effect of uric acid for the CL intensity, we developed a rapid, simple, and highly sensitive CL platform for uric acid detection. The linear range was 5-80 µM and the detection limit (LOD) for uric acid was 3.11 µM (S/N = 3). This work expanded the application of MoO<sub>3-x</sub> NPs in the CL field and developed a simple and highly sensitive CL sensing system to detect UA in human saliva.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":null,"pages":null},"PeriodicalIF":5.3,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141896435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}