Microchimica Acta最新文献_第3页

Immunoelectrochemical assessment of human IgE in non-invasive samples of allergic individuals using PdNCs-labelled antibodies

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07083-3

Alejandro Rodríguez-Penedo, Estefanía Costa-Rama, Rosario Pereiro, Beatriz Fernández, M. Teresa Fernández-Abedul

The escalating global prevalence of allergies presents a substantial public health challenge. Immunoglobulin E (IgE) serves as a key biomarker for allergic diseases, often measured in blood serum by ELISA immunoassays. Despite recent interest in minimally invasive sampling of biological fluids, the low sample volumes and IgE concentrations demand highly sensitive methodologies, typically confined to centralized laboratories. In this article, a decentralizable approach based on competitive immunoassays using Pd nanocluster (PdNCs)-labelled antibodies for electrochemical detection is proposed. With this aim, PdNCs were successfully bioconjugated with an anti-hIgE antibody to perform competitive immunoassays. To improve the analytical capabilities of the methodology, disposable screen-printed carbon electrodes with dual working electrodes were used for enhancing precision. Prior electrodeposition of PdNCs at − 0.6 V for 90 s significantly improved sensitivity (7.1 µA g ng⁻¹) and lowered the limit of detection (LoD) to 0.3 ng g⁻¹ for PdNCs determination. The use of PdNCs as labels resulted in an improvement in the LoD for IgE determination. Calibration curves performed using competitive immunoassays for IgE determination, ranging from 10⁻⁵ to 10² ng g⁻¹, demonstrated sensitivity comparable to high-tech methods, with a LoD of 0.008 ng g⁻¹ for electrochemical measurements. Bimodal detection of Pd (linear sweep voltammetry and inductively coupled plasma–mass spectrometry) in various biological fluids (saliva, tears, nasal exudate, capillary blood, and blood serum) revealed significant differences in IgE levels between allergic and non-allergic individuals. Notably, capillary blood correlated strongly with serum blood, and a certain correlation has also been found with nasal exudate. The electrochemical approach, combining sensitivity and precision with non-invasive sampling, offers a simplified alternative for IgE determination in allergic disease.

Graphical Abstract

{"title":"Immunoelectrochemical assessment of human IgE in non-invasive samples of allergic individuals using PdNCs-labelled antibodies","authors":"Alejandro Rodríguez-Penedo, Estefanía Costa-Rama, Rosario Pereiro, Beatriz Fernández, M. Teresa Fernández-Abedul","doi":"10.1007/s00604-025-07083-3","DOIUrl":"10.1007/s00604-025-07083-3","url":null,"abstract":"<div><p>The escalating global prevalence of allergies presents a substantial public health challenge. Immunoglobulin E (IgE) serves as a key biomarker for allergic diseases, often measured in blood serum by ELISA immunoassays. Despite recent interest in minimally invasive sampling of biological fluids, the low sample volumes and IgE concentrations demand highly sensitive methodologies, typically confined to centralized laboratories. In this article, a decentralizable approach based on competitive immunoassays using Pd nanocluster (PdNCs)-labelled antibodies for electrochemical detection is proposed. With this aim, PdNCs were successfully bioconjugated with an anti-hIgE antibody to perform competitive immunoassays. To improve the analytical capabilities of the methodology, disposable screen-printed carbon electrodes with dual working electrodes were used for enhancing precision. Prior electrodeposition of PdNCs at − 0.6 V for 90 s significantly improved sensitivity (7.1 µA g ng⁻<sup>1</sup>) and lowered the limit of detection (LoD) to 0.3 ng g⁻<sup>1</sup> for PdNCs determination. The use of PdNCs as labels resulted in an improvement in the LoD for IgE determination. Calibration curves performed using competitive immunoassays for IgE determination, ranging from 10<sup>−5</sup> to 10<sup>2</sup> ng g<sup>−1</sup>, demonstrated sensitivity comparable to high-tech methods, with a LoD of 0.008 ng g<sup>−1</sup> for electrochemical measurements. Bimodal detection of Pd (linear sweep voltammetry and inductively coupled plasma–mass spectrometry) in various biological fluids (saliva, tears, nasal exudate, capillary blood, and blood serum) revealed significant differences in IgE levels between allergic and non-allergic individuals. Notably, capillary blood correlated strongly with serum blood, and a certain correlation has also been found with nasal exudate. The electrochemical approach, combining sensitivity and precision with non-invasive sampling, offers a simplified alternative for IgE determination in allergic disease.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://link.springer.com/content/pdf/10.1007/s00604-025-07083-3.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638632","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cadmium telluride quantum dot-MXene composite–based electrochemical sensing platform for simultaneous determination of rutin and quercetin in foods

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07092-2

Lin Li, Lanlan Liu, Jianmei Zhou, Chen Gu, Xiongzhi Wu, Chenghong Lei, Liqiang Yan

A simple and rapid electrochemical method based on a composite of cadmium telluride quantum dots (CdTe QDs) and MXene is developed for the simultaneous determination of rutin and quercetin in food samples. The CdTe QD-MXene composite is synthesized via the in situ growth of CdTe QDs on MXene, which serves as a carrier and enhances electrical conductivity. Incorporating CdTe QDs into MXene interlayers effectively prevents agglomeration in MXene and provides more active sites for electrochemical determination. The developed electrochemical method can successfully determine rutin and quercetin, both individually and simultaneously, in aqueous solutions while achieving high stability and selectivity. Notably, the prepared sensor exhibits limits of detection of 3.300 × 10⁻⁸ and 3.268 × 10⁻⁷ M for the simultaneous determination of rutin and quercetin, respectively. Moreover, the sensing platform is used for the determination of rutin in buckwheat, locust rice, and apples, with results well comparable to those obtained using ultraviolet spectroscopy. Finally, the proposed sensor is employed to monitor the hydrolysis of rutin into quercetin in buckwheat using an electrochemical method for the first time. This study provides new ideas for the application of electrochemical sensors in food and drug science.

Graphical Abstract

{"title":"Cadmium telluride quantum dot-MXene composite–based electrochemical sensing platform for simultaneous determination of rutin and quercetin in foods","authors":"Lin Li, Lanlan Liu, Jianmei Zhou, Chen Gu, Xiongzhi Wu, Chenghong Lei, Liqiang Yan","doi":"10.1007/s00604-025-07092-2","DOIUrl":"10.1007/s00604-025-07092-2","url":null,"abstract":"<div><p> A simple and rapid electrochemical method based on a composite of cadmium telluride quantum dots (CdTe QDs) and MXene is developed for the simultaneous determination of rutin and quercetin in food samples. The CdTe QD-MXene composite is synthesized via the in situ growth of CdTe QDs on MXene, which serves as a carrier and enhances electrical conductivity. Incorporating CdTe QDs into MXene interlayers effectively prevents agglomeration in MXene and provides more active sites for electrochemical determination. The developed electrochemical method can successfully determine rutin and quercetin, both individually and simultaneously, in aqueous solutions while achieving high stability and selectivity. Notably, the prepared sensor exhibits limits of detection of 3.300 × 10<sup>−8</sup> and 3.268 × 10<sup>−7</sup> M for the simultaneous determination of rutin and quercetin, respectively. Moreover, the sensing platform is used for the determination of rutin in buckwheat, locust rice, and apples, with results well comparable to those obtained using ultraviolet spectroscopy. Finally, the proposed sensor is employed to monitor the hydrolysis of rutin into quercetin in buckwheat using an electrochemical method for the first time. This study provides new ideas for the application of electrochemical sensors in food and drug science.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrochemical sensing of caffeic acid on natural biomass-pyrrole-functionalized magnetic biochar (PFMB) as promising SPE material

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-18 DOI: 10.1007/s00604-025-07087-z

Imen Abidli, Mohamed Bououdina, Latifa Latrous, Adel Megriche

A peanut shell-modified screen-printed carbon electrode (SPE) was developed for the sensing of caffeic acid (CA) in saliva samples using cheap miniaturized analyzer composed of a laptop and an electrochemical workstation. Peanut shells, sourced from abundant biomass residues, were used to fabricate magnetic biochar (MB) and pyrrole-functionalized magnetic biochar (PFMB) with varying pyrrole/Fe ratios through a hydrothermal process. The surface morphology and electrochemical properties of the synthesized PFMB material were analyzed using XRD, FTIR, Raman, SEM, VSM, cyclic voltammetry, and differential pulse voltammetry techniques. The PFMB-modified SPE displayed excellent electrocatalytic response towards CA in a wide linear range from 10 to 600 μM with a low limit of detection of 0.08 μM. The enhanced electrocatalytic response could be ascribed to the synergistic effect of pyrrole-functionalized biochar and Fe₃O₄ on the newly designed probe. Moreover, the fabricated sensor was successfully utilized for real-time detection of CA in various samples. Quantum chemical modeling was performed to confirm the relevant findings to clarify the structure–activity relationship of CA adsorption on biochar.

Graphical Abstract

{"title":"Electrochemical sensing of caffeic acid on natural biomass-pyrrole-functionalized magnetic biochar (PFMB) as promising SPE material","authors":"Imen Abidli, Mohamed Bououdina, Latifa Latrous, Adel Megriche","doi":"10.1007/s00604-025-07087-z","DOIUrl":"10.1007/s00604-025-07087-z","url":null,"abstract":"<div><p>A peanut shell-modified screen-printed carbon electrode (SPE) was developed for the sensing of caffeic acid (CA) in saliva samples using cheap miniaturized analyzer composed of a laptop and an electrochemical workstation. Peanut shells, sourced from abundant biomass residues, were used to fabricate magnetic biochar (MB) and pyrrole-functionalized magnetic biochar (PFMB) with varying pyrrole/Fe ratios through a hydrothermal process. The surface morphology and electrochemical properties of the synthesized PFMB material were analyzed using XRD, FTIR, Raman, SEM, VSM, cyclic voltammetry, and differential pulse voltammetry techniques. The PFMB-modified SPE displayed excellent electrocatalytic response towards CA in a wide linear range from 10 to 600 μM with a low limit of detection of 0.08 μM. The enhanced electrocatalytic response could be ascribed to the synergistic effect of pyrrole-functionalized biochar and Fe<sub>3</sub>O<sub>4</sub> on the newly designed probe. Moreover, the fabricated sensor was successfully utilized for real-time detection of CA in various samples. Quantum chemical modeling was performed to confirm the relevant findings to clarify the structure–activity relationship of CA adsorption on biochar.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143645537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Facile preparation of polymer dots for tetracycline and Al3+ detection and exploration of anti-counterfeiting applications via the fluorescence “ON–OFF-ON” strategy

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-07093-1

Zhonghao Li, Zherui Wan, Xiaoxu Cui, Qi Chen, Yue Hua, Guang Yang

Polymer dots (PT-PDs) were obtained through a “synthesis-modification integration” method by using polyethyleneimine (PEI) and tartaric acid (TA) as raw materials. The designed PT-PDs displayed a nanoscale structure with an average size of 1.6 nm, and bright blue fluorescence (FL) emission with a fluorescence quantum yield (QY) of 14.3%. Moreover, the PT-PDs were used as a sensing platform for the sensitive and quantitative detection of tetracyclines (TCs) and Al³⁺ via fluorescence quenching and recovery. The LODs for tetracycline hydrochloride (TCH), doxycycline (DOX), and Al³⁺ were 94.8 nM, 76.7 nM, and 177.8 nM, respectively. In addition, PT-PDs incorporated with polyacrylamide were used for the recognition of TCs and Al³⁺ in a portable manner on the basis of the fluorescence “ON–OFF-ON” strategy, which revealed great application in the field of anti-counterfeiting and encryption.

Graphical Abstract

{"title":"Facile preparation of polymer dots for tetracycline and Al3+ detection and exploration of anti-counterfeiting applications via the fluorescence “ON–OFF-ON” strategy","authors":"Zhonghao Li, Zherui Wan, Xiaoxu Cui, Qi Chen, Yue Hua, Guang Yang","doi":"10.1007/s00604-025-07093-1","DOIUrl":"10.1007/s00604-025-07093-1","url":null,"abstract":"<div><p>Polymer dots (PT-PDs) were obtained through a “synthesis-modification integration” method by using polyethyleneimine (PEI) and tartaric acid (TA) as raw materials. The designed PT-PDs displayed a nanoscale structure with an average size of 1.6 nm, and bright blue fluorescence (FL) emission with a fluorescence quantum yield (QY) of 14.3%. Moreover, the PT-PDs were used as a sensing platform for the sensitive and quantitative detection of tetracyclines (TCs) and Al<sup>3+</sup> via fluorescence quenching and recovery. The LODs for tetracycline hydrochloride (TCH), doxycycline (DOX), and Al<sup>3+</sup> were 94.8 nM, 76.7 nM, and 177.8 nM, respectively. In addition, PT-PDs incorporated with polyacrylamide were used for the recognition of TCs and Al<sup>3+</sup> in a portable manner on the basis of the fluorescence “ON–OFF-ON” strategy, which revealed great application in the field of anti-counterfeiting and encryption.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143632386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Casein-Tb-Ti3C2 quantum dots ratiometric fluorescence probe for the detection of ciprofloxacin

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-07107-y

Chong Wang, Huixin Wang, Qingting Ni, Wenjuan Zhou, Dan Liu

A water-stable ratiometric fluorescence probe based on casein-Tb-Ti₃C₂ quantum dots was developed for the highly sensitive and selective detection of ciprofloxacin. With the increase in ciprofoxacin concentration, the fluorescence intensity at 440 nm remained relatively stable, while the fluorescence emission at 543 nm exhibited progressive enhancement. This may be attributed to ciprofloxacin being able to effectively coordinate with Tb³⁺ ions while displacing surrounding water molecules, leading to enhanced luminescence. Thus, the I₅₄₃/I₄₄₀ ratio was utilized as an indicator for monitoring changes in ciprofloxacin concentration. The ratiometric fluorescence probe demonstrated excellent linearity across a concentration range 0.1–800 µM and exhibited high sensitivity, achieving a detection limit of 0.018 μM for ciprofloxacin. Furthermore, the ratiometric fluorescence probe was successfully employed for the detection of ciprofloxacin in pork, beef, fish, honey, eggs, and milk samples, achieving recoveries that ranged from 76.5 to 123%. Owing to its advantages of high sensitivity and excellent selectivity, the ratiometric fluorescence probe demonstrates significant potential for practical applications in ciprofloxacin detection.

Graphical abstract

{"title":"Casein-Tb-Ti3C2 quantum dots ratiometric fluorescence probe for the detection of ciprofloxacin","authors":"Chong Wang, Huixin Wang, Qingting Ni, Wenjuan Zhou, Dan Liu","doi":"10.1007/s00604-025-07107-y","DOIUrl":"10.1007/s00604-025-07107-y","url":null,"abstract":"<div><p> A water-stable ratiometric fluorescence probe based on casein-Tb-Ti<sub>3</sub>C<sub>2</sub> quantum dots was developed for the highly sensitive and selective detection of ciprofloxacin. With the increase in ciprofoxacin concentration, the fluorescence intensity at 440 nm remained relatively stable, while the fluorescence emission at 543 nm exhibited progressive enhancement. This may be attributed to ciprofloxacin being able to effectively coordinate with Tb<sup>3+</sup> ions while displacing surrounding water molecules, leading to enhanced luminescence. Thus, the <i>I</i><sub>543</sub>/<i>I</i><sub>440</sub> ratio was utilized as an indicator for monitoring changes in ciprofloxacin concentration. The ratiometric fluorescence probe demonstrated excellent linearity across a concentration range 0.1–800 µM and exhibited high sensitivity, achieving a detection limit of 0.018 μM for ciprofloxacin. Furthermore, the ratiometric fluorescence probe was successfully employed for the detection of ciprofloxacin in pork, beef, fish, honey, eggs, and milk samples, achieving recoveries that ranged from 76.5 to 123%. Owing to its advantages of high sensitivity and excellent selectivity, the ratiometric fluorescence probe demonstrates significant potential for practical applications in ciprofloxacin detection.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638630","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-17 DOI: 10.1007/s00604-025-06997-2

Chao Guo, Tongnian Gu, Shao-Hua Wen, Yuan Dang, Yuanzhen Zhou, Junping Ma, Sha Yu

An intramolecular enhanced entropy-driven DNA amplifier-tethered gold nanoparticle (DNA-Au) nanodevice has been designed for highly sensitive in situ imaging of messenger ribonucleic acid (mRNA) in living cells. The DNA amplifier is immobilized on a same AuNP and the initial fluorescence of DNA-Au nanodevice is quenched. Upon internalized into the target cancer cells, the nanodevice can be activated by endogenous TK1 mRNA, and promptly release the fluorophore via the intramolecular enhanced DNA strand displacement reaction. The decreasing distance and increasing local concentration of the probes via intramolecular reaction can significantly improve the reaction kinetics of DNA-Au nanodevice, thus achieving the highly sensitive imaging of TK1 mRNA. The excellent sensitivity and selectivity allow the DNA-Au nanodevice to accurately discriminate different cell lines and monitor the variations in intracellular TK1 mRNA expression levels via fluorescence imaging. Therefore, the proposed intramolecular enhanced entropy-driven DNA-Au nanodevice will afford a reliable approach for accurate determination of mRNA in molecular diagnostic systems.

Graphical Abstract

{"title":"Intramolecular enhanced entropy-driven DNA-Au nanodevice for mRNA imaging in living cells","authors":"Chao Guo, Tongnian Gu, Shao-Hua Wen, Yuan Dang, Yuanzhen Zhou, Junping Ma, Sha Yu","doi":"10.1007/s00604-025-06997-2","DOIUrl":"10.1007/s00604-025-06997-2","url":null,"abstract":"<div><p>An intramolecular enhanced entropy-driven DNA amplifier-tethered gold nanoparticle (DNA-Au) nanodevice has been designed for highly sensitive in situ imaging of messenger ribonucleic acid (mRNA) in living cells. The DNA amplifier is immobilized on a same AuNP and the initial fluorescence of DNA-Au nanodevice is quenched. Upon internalized into the target cancer cells, the nanodevice can be activated by endogenous TK1 mRNA, and promptly release the fluorophore via the intramolecular enhanced DNA strand displacement reaction. The decreasing distance and increasing local concentration of the probes via intramolecular reaction can significantly improve the reaction kinetics of DNA-Au nanodevice, thus achieving the highly sensitive imaging of TK1 mRNA. The excellent sensitivity and selectivity allow the DNA-Au nanodevice to accurately discriminate different cell lines and monitor the variations in intracellular TK1 mRNA expression levels via fluorescence imaging. Therefore, the proposed intramolecular enhanced entropy-driven DNA-Au nanodevice will afford a reliable approach for accurate determination of mRNA in molecular diagnostic systems.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143638631","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Capillary-driven distance-based paper analytical devices for albumin protein and glucose quantification in human whole blood

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-15 DOI: 10.1007/s00604-025-07079-z

Kawin Khachornsakkul, Thithawat Trakoolwilaiwan, Ruben Del-Rio-Ruiz, Sameer Sonkusale, Tapparath Leelasattarathkul

This study presents a simple and inexpensive distance-based paper analytical device (dPAD) for plasma separation from whole blood samples and its application in monitoring albumin protein and glucose levels using colorimetric and fluorescent distance methods. The developed dPAD consists of a sample zone, a separation zone with hydrophobic wax-patterned lines, a pretreatment zone, and a straight zone channel pre-deposited with chemical reagents for both albumin protein and glucose quantification. Plasma separation relies on the capillarity-driven different flow velocities of blood cells and plasma with varying hydrophilicity in the paper channel. Remarkably, the blood cells are trapped in the separation channel of the device, while plasma can be separated and subsequently flow with a buffer solution to the detection zone by capillary force. Target analyte in plasma content then reacts with its specific reagents, resulting in the change in the color or fluorescent distance signal. Our sensor exhibited remarkable accuracy and precision for the detection of albumin protein and glucose in whole blood samples with an acceptable recovery range between 99.94 and 101.65% and the highest relative standard deviation (RSD) of 4.49%. Furthermore, the results indicated no significant differences between our method and conventional methods for albumin protein and glucose determination in whole blood samples. Additionally, to the best of our knowledge, this method is the first time for the development of the fluorescent dPAD sensor for glucose monitoring. It is also the first demonstration to use a dPAD sensor for the direct detection of both albumin protein and glucose levels in whole blood. Hence, despite its simplicity, the concept offers a more cost-effective and accessible method for plasma separation from whole blood and subsequent albumin protein or and glucose detection. Moreover, it can be extended for further advancements in POC analytical sensing.

Graphical Abstract

{"title":"Capillary-driven distance-based paper analytical devices for albumin protein and glucose quantification in human whole blood","authors":"Kawin Khachornsakkul, Thithawat Trakoolwilaiwan, Ruben Del-Rio-Ruiz, Sameer Sonkusale, Tapparath Leelasattarathkul","doi":"10.1007/s00604-025-07079-z","DOIUrl":"10.1007/s00604-025-07079-z","url":null,"abstract":"<div><p>This study presents a simple and inexpensive distance-based paper analytical device (dPAD) for plasma separation from whole blood samples and its application in monitoring albumin protein and glucose levels using colorimetric and fluorescent distance methods. The developed dPAD consists of a sample zone, a separation zone with hydrophobic wax-patterned lines, a pretreatment zone, and a straight zone channel pre-deposited with chemical reagents for both albumin protein and glucose quantification. Plasma separation relies on the capillarity-driven different flow velocities of blood cells and plasma with varying hydrophilicity in the paper channel. Remarkably, the blood cells are trapped in the separation channel of the device, while plasma can be separated and subsequently flow with a buffer solution to the detection zone by capillary force. Target analyte in plasma content then reacts with its specific reagents, resulting in the change in the color or fluorescent distance signal. Our sensor exhibited remarkable accuracy and precision for the detection of albumin protein and glucose in whole blood samples with an acceptable recovery range between 99.94 and 101.65% and the highest relative standard deviation (RSD) of 4.49%. Furthermore, the results indicated no significant differences between our method and conventional methods for albumin protein and glucose determination in whole blood samples. Additionally, to the best of our knowledge, this method is the first time for the development of the fluorescent dPAD sensor for glucose monitoring. It is also the first demonstration to use a dPAD sensor for the direct detection of both albumin protein and glucose levels in whole blood. Hence, despite its simplicity, the concept offers a more cost-effective and accessible method for plasma separation from whole blood and subsequent albumin protein or and glucose detection. Moreover, it can be extended for further advancements in POC analytical sensing. </p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629697","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A novel smartphone-mediated ratiometric fluorescence imprinting sensor based on boric acid-functionalized Eu-MOF for the detection of horseradish peroxidase

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-14 DOI: 10.1007/s00604-025-07074-4

Minghao Ma, Wei Hu, Shengdong Luo, Fubin Pei, Wu Lei, Jiang Wang, Zhaoyang Tong, Bing Liu, Bin Du, Qingli Hao, Xihui Mu

Horseradish peroxidase (HRP) was used as a model glycoprotein, and a molecularly imprinted ratiometric fluorescence sensor based on a smartphone (NEB@MIP) was constructed using the sol–gel method for the fluorescence and visual detection of HRP. The sensor consisted of boronic acid-functionalized metal–organic frameworks (Eu-MOF-B(OH)₂) and nitrogen-doped carbon dots (N-CDs). The Eu-MOF-B(OH)₂ surface can not only load abundant N-CDs but also covalently bind with HRP through its boronic acid groups. The NEB@MIP exhibited two fluorescence emission peaks at 450 nm and 616 nm. When HRP was present, the fluorescence was quenched due to the internal filtering effect (IFE), but the quenching of N-CDs was more pronounced. Furthermore, the concentration of HRP in the range 0.05–10 µM showed a good linear relationship with the ratio of fluorescence intensity at 616 nm and 450 nm, with a detection limit (LOD) of 0.01 µM. Meanwhile, the sensor displayed a noticeable change in fluorescence color under different concentrations of HRP targets. Moreover, the sensor achieved satisfactory results in detecting simulated real samples, with recoveries ranging from 92.0% to 98.5% and RSDs between 1.5% and 3.3%. The detection platform based on the smartphone also performed well when detecting HRP in simulated real samples. Thus, this work provided a new approach for the portable detection of HRP. The method provides a new idea for the combination of ratiometric fluorescence molecular imprinting of glycoproteins and the portable detection platform of smart phones.

Graphical Abstract

{"title":"A novel smartphone-mediated ratiometric fluorescence imprinting sensor based on boric acid-functionalized Eu-MOF for the detection of horseradish peroxidase","authors":"Minghao Ma, Wei Hu, Shengdong Luo, Fubin Pei, Wu Lei, Jiang Wang, Zhaoyang Tong, Bing Liu, Bin Du, Qingli Hao, Xihui Mu","doi":"10.1007/s00604-025-07074-4","DOIUrl":"10.1007/s00604-025-07074-4","url":null,"abstract":"<div><p> Horseradish peroxidase (HRP) was used as a model glycoprotein, and a molecularly imprinted ratiometric fluorescence sensor based on a smartphone (NEB@MIP) was constructed using the sol–gel method for the fluorescence and visual detection of HRP. The sensor consisted of boronic acid-functionalized metal–organic frameworks (Eu-MOF-B(OH)<sub>2</sub>) and nitrogen-doped carbon dots (N-CDs). The Eu-MOF-B(OH)<sub>2</sub> surface can not only load abundant N-CDs but also covalently bind with HRP through its boronic acid groups. The NEB@MIP exhibited two fluorescence emission peaks at 450 nm and 616 nm. When HRP was present, the fluorescence was quenched due to the internal filtering effect (IFE), but the quenching of N-CDs was more pronounced. Furthermore, the concentration of HRP in the range 0.05–10 µM showed a good linear relationship with the ratio of fluorescence intensity at 616 nm and 450 nm, with a detection limit (LOD) of 0.01 µM. Meanwhile, the sensor displayed a noticeable change in fluorescence color under different concentrations of HRP targets. Moreover, the sensor achieved satisfactory results in detecting simulated real samples, with recoveries ranging from 92.0% to 98.5% and RSDs between 1.5% and 3.3%. The detection platform based on the smartphone also performed well when detecting HRP in simulated real samples. Thus, this work provided a new approach for the portable detection of HRP. The method provides a new idea for the combination of ratiometric fluorescence molecular imprinting of glycoproteins and the portable detection platform of smart phones.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Significantly improved catalytic activity of copper nanocrystal by introducing Ti3C2TX and arginine and serine-functionalized graphene quantum dot for colorimetric detection of H2O2

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-13 DOI: 10.1007/s00604-025-07027-x

Ji Min, Li Ruiyi, Li Zaijun

Copper nanocrystal has been widely used as nanozyme for construction of optical sensing platforms because of low cost, special catalysis, and high stability. However, low catalytic activity limits further applications in bioanalysis. This study reports one way for improving the catalytic activity of copper nanocrystal by introducing Ti₃C₂T_X and arginine and serine-functionalized graphene quantum dot (RSGQD). Cu²⁺ was reduced by RSGQD to produce copper nanocrystal, which was immobilized on Ti₃C₂T_X sheet via π-π stacking and self-assembly. The resulted Ti₃C₂T_X/Cu-RSGQD shows a three-dimensional structure composing of small copper nanocrystals with an average particle size of 18.1 ± 1.7 nm and Ti₃C₂T_X sheets. The introduction of Ti₃C₂T_X and RSGQD improves the catalytic activity due to good conductivity of Ti₃C₂T_X and formation of Ti₃C₂T_X/RSGQD/Cu Schottky heterojunction. The peroxidase-like and oxidase-like specific activities reach 591.61 U mg⁻¹ and 105.2 U mg⁻¹. Based on the catalysis of Ti₃C₂T_X/Cu-RSGQD towards oxidation of 3,3′,5,5′-tetramethylbenzidine into a blue product, a sensitive method was developed for colorimetric detection of H₂O₂. The absorbance linearly increases with increasing H₂O₂ concentration between 0 and 50 μM with a detection limit of 0.0032 μM (S/N = 3). The sensitivity is better than that of other reported analytical methods. It has been contentedly applied in colorimetric detection of H₂O₂ in food.

Graphical abstract

{"title":"Significantly improved catalytic activity of copper nanocrystal by introducing Ti3C2TX and arginine and serine-functionalized graphene quantum dot for colorimetric detection of H2O2","authors":"Ji Min, Li Ruiyi, Li Zaijun","doi":"10.1007/s00604-025-07027-x","DOIUrl":"10.1007/s00604-025-07027-x","url":null,"abstract":"<div><p>Copper nanocrystal has been widely used as nanozyme for construction of optical sensing platforms because of low cost, special catalysis, and high stability. However, low catalytic activity limits further applications in bioanalysis. This study reports one way for improving the catalytic activity of copper nanocrystal by introducing Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> and arginine and serine-functionalized graphene quantum dot (RSGQD). Cu<sup>2+</sup> was reduced by RSGQD to produce copper nanocrystal, which was immobilized on Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> sheet via π-π stacking and self-assembly. The resulted Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub>/Cu-RSGQD shows a three-dimensional structure composing of small copper nanocrystals with an average particle size of 18.1 ± 1.7 nm and Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> sheets. The introduction of Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> and RSGQD improves the catalytic activity due to good conductivity of Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub> and formation of Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub>/RSGQD/Cu Schottky heterojunction. The peroxidase-like and oxidase-like specific activities reach 591.61 U mg<sup>−1</sup> and 105.2 U mg<sup>−1</sup>. Based on the catalysis of Ti<sub>3</sub>C<sub>2</sub>T<sub>X</sub>/Cu-RSGQD towards oxidation of 3,3′,5,5′-tetramethylbenzidine into a blue product, a sensitive method was developed for colorimetric detection of H<sub>2</sub>O<sub>2</sub>. The absorbance linearly increases with increasing H<sub>2</sub>O<sub>2</sub> concentration between 0 and 50 μM with a detection limit of 0.0032 μM (S/N = 3). The sensitivity is better than that of other reported analytical methods. It has been contentedly applied in colorimetric detection of H<sub>2</sub>O<sub>2</sub> in food.</p><h3>Graphical abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143602367","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A sensitive electrochemical biosensor based on Exo III cyclic amplification strategy for Phaeocystis globosa detection

IF 5.3 2区化学 Q1 CHEMISTRY, ANALYTICAL

Microchimica Acta

Pub Date : 2025-03-13 DOI: 10.1007/s00604-025-07095-z

Hongjie Liu, Hao Fu, Yibo Zhang, Shaopeng Wang, Kedi Yang, Liwei Wang

Phaeocystis globosa (P. globosa), a main culprit of harmful algal blooms (HABs), is highly prone to blocking the inlet filter screens of nuclear power cold sources, thus posing a significant threat to nuclear power safety. However, existing methods for P. globosa detection fail to achieve rapid and on-site monitoring of single-cell densities prior to bloom outbreaks, limiting timely defensive measures. In this study, we developed a novel biosensor platform for efficient P. globosa detection, leveraging an Exo III-assisted signal amplification strategy to significantly enhance sensitivity and selectivity. The biosensor achieved an ultra-low limit of detection (LOD) of 268.91 fg µL⁻¹ (3119 cells L⁻¹), far below the benchmark concentration for P. globosa blooms (10⁷ cells L⁻¹), and demonstrated a wide linear detection range from 500 fg µL⁻¹ to 10 ng µL⁻¹. Furthermore, the biosensor’s accuracy and reliability were validated through comparative analysis with droplet digital PCR (ddPCR) using actual samples from the Beibu Gulf of China, revealing a low risk of P. globosa blooms in the region at the sampling time. This study represents a significant advancement in HAB monitoring by providing a highly sensitive, rapid, and field-deployable tool for early warning of P. globosa blooms. The biosensor’s innovative design and performance address critical gaps in current detection methods, offering practical implications for safeguarding coastal nuclear power facilities and protecting marine ecosystems.

{"title":"A sensitive electrochemical biosensor based on Exo III cyclic amplification strategy for Phaeocystis globosa detection","authors":"Hongjie Liu, Hao Fu, Yibo Zhang, Shaopeng Wang, Kedi Yang, Liwei Wang","doi":"10.1007/s00604-025-07095-z","DOIUrl":"10.1007/s00604-025-07095-z","url":null,"abstract":"<p><i>Phaeocystis globosa</i> (<i>P. globosa</i>), a main culprit of harmful algal blooms (HABs), is highly prone to blocking the inlet filter screens of nuclear power cold sources, thus posing a significant threat to nuclear power safety. However, existing methods for <i>P. globosa</i> detection fail to achieve rapid and on-site monitoring of single-cell densities prior to bloom outbreaks, limiting timely defensive measures. In this study, we developed a novel biosensor platform for efficient <i>P. globosa</i> detection, leveraging an Exo III-assisted signal amplification strategy to significantly enhance sensitivity and selectivity. The biosensor achieved an ultra-low limit of detection (LOD) of 268.91 fg µL<sup>−1</sup> (3119 cells L<sup>−1</sup>), far below the benchmark concentration for <i>P. globosa</i> blooms (10<sup>7</sup> cells L<sup>−1</sup>), and demonstrated a wide linear detection range from 500 fg µL<sup>−1</sup> to 10 ng µL<sup>−1</sup>. Furthermore, the biosensor’s accuracy and reliability were validated through comparative analysis with droplet digital PCR (ddPCR) using actual samples from the Beibu Gulf of China, revealing a low risk of <i>P. globosa</i> blooms in the region at the sampling time. This study represents a significant advancement in HAB monitoring by providing a highly sensitive, rapid, and field-deployable tool for early warning of <i>P. globosa</i> blooms. The biosensor’s innovative design and performance address critical gaps in current detection methods, offering practical implications for safeguarding coastal nuclear power facilities and protecting marine ecosystems.</p>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 4","pages":""},"PeriodicalIF":5.3,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143612128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0