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Pharmacokinetic predictions of ROS-mediated targets and genotoxin combinations via multiple ligand simultaneous docking and ROS evaluation in vitro using HepG2 cell lines. 利用 HepG2 细胞系,通过多配体同时对接和 ROS 体外评估,对 ROS 介导的靶标和基因毒素组合进行药代动力学预测。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-14 DOI: 10.1007/s13205-024-04109-0
C P Sri Snehaa, Praveen Kumar Issac, Palanisamy Rajaguru, Velan Pugalenthi

Although combination therapy is known for its high efficacy, reduced side effects and drug resistance, toxicity remains a major drawback. Some of the genes are likely to induce hepatotoxicity through ROS-mediated mechanisms when a drug is metabolized alone or in combination in the liver. To address this, we have developed a scientific approach to predict the toxicity of different genotoxin combinations and validate their interactions with various targets. The current study is an extensive study of our previous set of in vivo rat liver microarray data processed using R studio for their functional analysis. About five combinations of genotoxins such as CPT/ETP, CPT/CPL, ETP/CPL, CP/CPT and EES/CP along with their differential gene expression targeting Chemical carcinogenesis-ROS are chosen for this study. We aim to examine the binding affinity of different genotoxin combinations using in silico multiple ligand simultaneous docking (MLSD) and are then bio-evaluated for cytotoxicity in vitro using human hepatocellular carcinoma cell lines (HepG2) with the MTT assay. As a result, dose-response cytotoxicity with its strength of interactions and a significant variance in ROS levels in the treated cells is observed compared to their IC50 values. Out of 5 combinations such as CPT/CPL, ETP/CPL and EES/CP are found not only to be significantly cytotoxic but also induce oxidative stress specifically above their IC50 values with good and moderate binding interactions ensuring their toxicity. On the contrary, the safe combinations are found to be CTP/ETP and CP/CPT possibly with no and tolerable adverse effects standing as preliminary information for researchers in drug design and development.

尽管联合疗法以疗效高、副作用小和耐药性低而著称,但毒性仍然是其主要缺点。当药物在肝脏中单独或联合代谢时,其中一些基因很可能通过 ROS 介导的机制诱发肝毒性。为此,我们开发了一种科学方法来预测不同基因毒素组合的毒性,并验证它们与各种靶点的相互作用。目前的研究是对我们之前使用 R studio 处理的一组体内大鼠肝脏微阵列数据进行功能分析的广泛研究。本研究选择了五种基因毒素组合,如 CPT/ETP、CPT/CPL、ETP/CPL、CP/CPT 和 EES/CP,以及它们针对化学癌变-ROS 的不同基因表达。我们的目的是利用硅学多配体同时对接(MLSD)技术研究不同基因毒素组合的结合亲和力,然后使用 MTT 法对人肝癌细胞株(HepG2)进行体外细胞毒性生物评估。结果,观察到剂量-反应细胞毒性及其相互作用的强度,以及与它们的 IC50 值相比,处理细胞中 ROS 水平的显著差异。在 CPT/CPL、ETP/CPL 和 EES/CP 等 5 种组合中,发现它们不仅具有显著的细胞毒性,而且还能诱导氧化应激,其毒性明显高于其 IC50 值,其良好和适度的结合相互作用确保了它们的毒性。相反,CTP/ETP 和 CP/CPT 被认为是安全的组合,它们可能不会产生任何不良影响,而且是可以承受的,这为研究人员进行药物设计和开发提供了初步信息。
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引用次数: 0
Editorial Expression of Concern: Two species of Ulva inhibits the progression of cervical cancer cells SiHa by means of autophagic cell death induction. 社论表达关注:两种莼菜通过诱导自噬细胞死亡抑制宫颈癌细胞SiHa的进展。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-24 DOI: 10.1007/s13205-024-04115-2
Asmita Pal, Preeti Verma, Subhabrata Paul, Indira Majumder, Rita Kundu
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引用次数: 0
Enhancing glioblastoma cytotoxicity through encapsulating O6-benzylguanine and temozolomide in PEGylated liposomal nanocarrier: an in vitro study. 通过在 PEG 化脂质体纳米载体中封装 O6-苄基鸟嘌呤和替莫唑胺来增强胶质母细胞瘤的细胞毒性:一项体外研究。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1007/s13205-024-04123-2
Manasa Manjunath Hegde, Pranoti Palkar, Sadhana P Mutalik, Srinivas Mutalik, Jayant Sastri Goda, B S Satish Rao

Glioblastoma (GBM) (grade IV glioma) is the most fatal brain tumor, with a median survival of just 14 months despite current treatments. Temozolomide (TMZ), an alkylating agent used with radiation, faces challenges such as systemic toxicity, poor absorption, and drug resistance. To enhance TMZ effectiveness, we developed poly(ethylene glycol) (PEG) liposomes co-loaded with TMZ and O6-benzylguanine (O6-BG) for targeted glioma therapy. These liposomes, prepared using the thin-layer hydration method, had an average size of 146.33 ± 6.75 nm and a negative zeta potential (-49.6 ± 3.1 mV). Drug release was slower at physiological pH, with 66.84 ± 4.62% of TMZ and 69.70 ± 2.88% of O6-BG released, indicating stability at physiological conditions. The liposomes showed significantly higher cellular uptake (p < 0.05) than the free dye. The dual drug-loaded liposomes exhibited superior cytotoxicity against U87 glioma cells, with a lower IC50 value (3.99µg/mL) than the free drug combination, demonstrating enhanced anticancer efficacy. The liposome formulation induced higher apoptosis (19.42 ± 3.5%) by causing sub-G0/G1 cell cycle arrest. The novelty of our study lies in co-encapsulating TMZ and O6-BG within PEGylated liposomes, effectively overcoming drug resistance and improving targeted delivery for glioma treatment.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04123-2.

胶质母细胞瘤(GBM)(IV 级胶质瘤)是最致命的脑肿瘤,尽管目前有多种治疗方法,但中位生存期仅为 14 个月。替莫唑胺(TMZ)是一种与放射线配合使用的烷化剂,它面临着全身毒性、吸收不良和耐药性等挑战。为了提高TMZ的疗效,我们开发了共载TMZ和O6-苄基鸟嘌呤(O6-BG)的聚乙二醇(PEG)脂质体,用于胶质瘤的靶向治疗。这些脂质体采用薄层水合法制备,平均尺寸为 146.33 ± 6.75 nm,Zeta 电位为负(-49.6 ± 3.1 mV)。在生理 pH 值下,药物释放速度较慢,TMZ 和 O6-BG 的释放量分别为 66.84 ± 4.62% 和 69.70 ± 2.88%,这表明脂质体在生理条件下是稳定的。脂质体的细胞吸收率(p 50 值(3.99µg/mL))明显高于游离药物组合,表明抗癌功效增强。脂质体制剂通过引起亚 G0/G1 细胞周期停滞,诱导更多细胞凋亡(19.42 ± 3.5%)。我们研究的新颖之处在于将TMZ和O6-BG共同包裹在PEG化脂质体中,有效克服了耐药性,提高了胶质瘤治疗的靶向给药效果:在线版本包含补充材料,可查阅 10.1007/s13205-024-04123-2。
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引用次数: 0
Discovery and characterization of novel lipopeptides produced by Virgibacillus massiliensis with biosurfactant and antimicrobial activities. 发现 Massiliensis 维吉巴菌产生的具有生物表面活性剂和抗菌活性的新型脂肽并确定其特性。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-04 DOI: 10.1007/s13205-024-04100-9
Badiaa Essghaier, Chahnez Naccache, Houda Ben-Miled, Filomena Mottola, Kamel Ben-Mahrez, Maha Mezghani Khemakhem, Lucia Rocco

The study aimed to evaluate the biosurfactants (BSs) production by SM-23 strain of Virgibacillus identified by phenotypical and WGS analysis as Virgibacillus massiliensis. We first demonstrated the lipopeptides production by Virgibacillus massiliensis specie and studied their biochemical and molecular analysis as well as their biological potential. The GC-MS analysis indicated that methyl.2-hyroxydodecanoate was the major fatty acid compound with 33.22%. The maximum BSs production was obtained in LB medium supplemented by 1% olive oil (v/v) at 30 °C and 5% NaCl with 1.92 g/l. The obtained results revealed the significant biosurfactants/bioemulsifier potential compared to triton X100 with E24 of 100%, and an emulsification stability SE of 83%. The lipopeptides types were identified by FTIR analysis. A strong antimicrobial action was observed by the produced lipopeptides by the agar diffusion method against E.coli, K. pneumoniae, S. aureus, Fusarium sp, Alternaria sp, and Phytophtora sp. The complete genome sequencing showed genes involved in the synthesis of multiple compounds identified as amphipathic cyclic lipopeptides such as locillomycin/locillomycin B/locillomycin C and bacillibactin. Our results highlighted significant lipopeptides properties displayed by V. massiliensis that can be exploited to develop a novel strategy in the formulation of natural biocidal and fungicidal agents.

Supplementary information: The online version contains supplementary material available at 10.1007/s13205-024-04100-9.

本研究旨在评估经表型和 WGS 分析鉴定为大肠维吉杆菌(Virgibacillus massiliensis)的 SM-23 株大肠维吉杆菌(Virgibacillus massiliensis)生产生物表面活性剂(BSs)的情况。我们首先证明了大肠维吉杆菌(Virgibacillus massiliensis)能产生脂肽,并研究了其生化和分子分析及其生物潜力。气相色谱-质谱分析表明,2-羟基十二酸甲酯是主要的脂肪酸化合物,占 33.22%。在 30 °C、5%NaCl、添加 1%橄榄油(v/v)的 LB 培养基中,BSs 的产量最大,为 1.92 克/升。研究结果表明,与 E24 为 100%、乳化稳定性 SE 为 83% 的 triton X100 相比,生物表面活性剂/生物乳化剂具有显著的潜力。通过傅立叶变换红外分析确定了脂肽的类型。通过琼脂扩散法,观察到所生产的脂肽对大肠杆菌、肺炎金葡菌、金黄色葡萄球菌、镰刀菌属、交替孢霉属和疫霉属有很强的抗菌作用。全基因组测序显示,有基因参与合成多种被鉴定为两性环脂肽的化合物,如洛钙霉素/洛钙霉素 B/洛钙霉素 C 和巴氏杀菌素。我们的研究结果突显了V. massiliensis所显示的重要脂肽特性,可以利用这些特性开发出一种新型战略,用于配制天然杀菌剂和杀真菌剂:在线版本包含补充材料,可查阅 10.1007/s13205-024-04100-9。
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引用次数: 0
Biological characterization of marine algae and its potent in vitro antioxidant, antimicrobial and larvicidal activity: a focus on Ulva lactuca Linnaeus 1753: 1163. 海洋藻类的生物学特征及其强大的体外抗氧化、抗菌和杀幼虫剂活性:重点关注乳莼菜(Ulva lactuca Linnaeus 1753: 1163)。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-05 DOI: 10.1007/s13205-024-04105-4
Jayanthi Kumaravelu, Deepak Paramasivam, Mohammed Rafi Shaik, Ajay Guru, Nathiya Thiyagarajulu, Manon Mani Vellingiri, Shaik Althaf Hussain

This study evaluated the biological characteristics of seaweeds Turbinaria ornata, Ulva lactuca, and Gracilaria crassa. Among the seaweeds tested, ethyl acetate extract of Ulva lactuca exhibited the highest antibacterial activity against Salmonella enterica, Staphylococcus aureus, and Pseudomonas aeruginosa. The phytochemical analysis of ULME and ULEA showed the presence of most of the tested phytochemicals, whereas only amino acids, tannins, glycosides, and carbohydrates were detected by ULHE. The DPPH scavenging property of U. lactuca exerted the maximum antioxidant property of 62.54% (ULME), 75.64% (ULEA), and 39.55% (ULHE), whereas the alpha amylase inhibitory property (µg/mL) of ULME, ULEA, and ULHE was, respectively, 80.99, 51.15, and 49.23. ULME, ULEA, and ULHE exhibited the greatest alpha-glucosidase inhibition, with IC50 values (g/mL) of 116.12, 45.59, and 170.10 correspondingly. ULEA also showed potent mosquito-larvicidal effects against Aedes aegypti larvae with the maximum lethal concentration values with LC50 and LC90 values (mg/mL) being 11.55 and 65.97, respectively. FTIR analysis of ULME, ULHE, and ULEA were found to have various functional groups, including alkanes, carboxylic acids, alkenes, alkynes, aldehydes, amides and alkanes, ketones, and aromatics, while HPLC revealed a strong peak at 4.760 retention time. In conclusion, Ulva lactuca, particularly its ethyl acetate extract, demonstrates significant antibacterial, antioxidant, and enzyme-inhibitory properties, highlighting its therapeutic and biotechnological potential. Its diverse phytochemicals and effective mosquito-larvicidal activity further support its broad application prospects.

本研究评估了海藻 Turbinaria ornata、Ulva lactuca 和 Gracilaria crassa 的生物特性。在测试的海藻中,乳莼的乙酸乙酯提取物对肠炎沙门氏菌、金黄色葡萄球菌和绿脓杆菌的抗菌活性最高。植物化学分析显示,ULME 和 ULEA 提取物含有大多数测试的植物化学物质,而 ULHE 提取物只检测到氨基酸、单宁、苷类和碳水化合物。U. lactuca 的 DPPH 清除率最高,分别为 62.54%(ULME)、75.64%(ULEA)和 39.55%(ULHE),而 ULME、ULEA 和 ULHE 的α-淀粉酶抑制率(µg/mL)分别为 80.99、51.15 和 49.23。ULME、ULEA 和 ULHE 对α-葡萄糖苷酶的抑制作用最大,IC50 值(克/毫升)分别为 116.12、45.59 和 170.10。ULEA 对埃及伊蚊幼虫也有很强的杀蚊虫效果,最大致死浓度值(LC50 和 LC90 值,毫克/毫升)分别为 11.55 和 65.97。傅立叶变换红外光谱分析发现,ULME、ULHE 和 ULEA 具有多种官能团,包括烷烃、羧酸、烯烃、炔烃、醛、酰胺和烷烃、酮和芳烃,而 HPLC 则在 4.760 保留时间处发现了一个强峰。总之,乳莼菜,尤其是其乙酸乙酯提取物,具有显著的抗菌、抗氧化和酶抑制特性,彰显了其治疗和生物技术潜力。其多样的植物化学成分和有效的杀蚊虫活性进一步支持了其广阔的应用前景。
{"title":"Biological characterization of marine algae and its potent in vitro antioxidant, antimicrobial and larvicidal activity: a focus on <i>Ulva lactuca</i> Linnaeus 1753: 1163.","authors":"Jayanthi Kumaravelu, Deepak Paramasivam, Mohammed Rafi Shaik, Ajay Guru, Nathiya Thiyagarajulu, Manon Mani Vellingiri, Shaik Althaf Hussain","doi":"10.1007/s13205-024-04105-4","DOIUrl":"10.1007/s13205-024-04105-4","url":null,"abstract":"<p><p>This study evaluated the biological characteristics of seaweeds <i>Turbinaria ornata</i>, <i>Ulva lactuca</i>, and <i>Gracilaria crassa</i>. Among the seaweeds tested, ethyl acetate extract of <i>Ulva lactuca</i> exhibited the highest antibacterial activity against <i>Salmonella enterica, Staphylococcus aureus,</i> and <i>Pseudomonas aeruginosa</i>. The phytochemical analysis of ULME and ULEA showed the presence of most of the tested phytochemicals, whereas only amino acids, tannins, glycosides, and carbohydrates were detected by ULHE. The DPPH scavenging property of <i>U. lactuca</i> exerted the maximum antioxidant property of 62.54% (ULME), 75.64% (ULEA), and 39.55% (ULHE), whereas the alpha amylase inhibitory property (µg/mL) of ULME, ULEA, and ULHE was, respectively, 80.99, 51.15, and 49.23. ULME, ULEA, and ULHE exhibited the greatest alpha-glucosidase inhibition, with IC<sub>50</sub> values (g/mL) of 116.12, 45.59, and 170.10 correspondingly. ULEA also showed potent mosquito-larvicidal effects against <i>Aedes aegypti</i> larvae with the maximum lethal concentration values with LC<sub>50</sub> and LC<sub>90</sub> values (mg/mL) being 11.55 and 65.97, respectively<i>.</i> FTIR analysis of ULME, ULHE, and ULEA were found to have various functional groups, including alkanes, carboxylic acids, alkenes, alkynes, aldehydes, amides and alkanes, ketones, and aromatics, while HPLC revealed a strong peak at 4.760 retention time. In conclusion, <i>Ulva lactuca</i>, particularly its ethyl acetate extract, demonstrates significant antibacterial, antioxidant, and enzyme-inhibitory properties, highlighting its therapeutic and biotechnological potential. Its diverse phytochemicals and effective mosquito-larvicidal activity further support its broad application prospects.</p>","PeriodicalId":7067,"journal":{"name":"3 Biotech","volume":"14 11","pages":"261"},"PeriodicalIF":2.6,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11455792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142387129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reappraisal of different agro-industrial waste for the optimization of cellulase production from Aspergillus niger ITV02 in a liquid medium using a Box-Benkhen design. 采用方框-本肯设计,重新评估不同农工业废弃物对黑曲霉 ITV02 在液体培养基中生产纤维素酶的优化作用。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-23 DOI: 10.1007/s13205-024-04120-5
Alejandra Miranda-Sosa, Sandra Del Moral, María Inés Infanzón-Rodriguez, María Guadalupe Aguilar-Uscanga

High-value metabolites, such as enzymes and biofuels, can be produced from various agro-industrial waste containing high percentages of cellulose and hemicellulose. Aspergillus niger ITV02 demonstrates high potential in cellulases production, the key enzyme for converting lignocellulosic materials into fermentable sugars to produce second-generation bioethanol (bioethanol 2G). This study evaluated five lignocellulosic residues of agricultural importance: sugarcane bagasse (SCB), sorghum bagasse (SB), corn stubble (CS), barley straw (BS) and rice husk (RH) as substrates for cellulase production. The temperature, pH and stirring conditions were optimized using a Box-Behnken design to identify the most suitable conditions for cellulase production while minimizing nitrogen concentrations. The results indicate that the best way of propagation A. niger ITV02 is through the use of spores as an inoculant, in conjunction with the use of materials with a high cellulose/lignin ratio, such as CS and SB for the generation of cellulases. These conditions promote the expression of cellulases towards β-glucosidase production, unlike materials with lower cellulose/lignin ratios like BS and RH, which exhibited lower cellulase activity. The optimal conditions for cellulase production by A. niger ITV02 were determined to be 33 °C, pH 5.3, and 200 rpm, resulting in a 1.7-fold increase in Exoglucanase (FPase activity) (from 0.127 to 0.215 U/mL). These findings demonstrate the potential to enhance FPase activity by utilizing substrates with high cellulose/lignin content and implementing optimal operational conditions without the need to raise the nitrogen content of the basal medium, thus mitigating the economic impact of cellulase production.

可从含有高比例纤维素和半纤维素的各种农工废料中生产高价值代谢物,如酶和生物燃料。黑曲霉 ITV02 具有生产纤维素酶的巨大潜力,而纤维素酶是将木质纤维素材料转化为可发酵糖以生产第二代生物乙醇(生物乙醇 2G)的关键酶。本研究对甘蔗渣(SCB)、高粱渣(SB)、玉米茬(CS)、大麦秸(BS)和稻壳(RH)这五种具有重要农业意义的木质纤维素残留物作为纤维素酶生产的底物进行了评估。采用方框-贝肯设计法对温度、pH 值和搅拌条件进行了优化,以确定最适合纤维素酶生产的条件,同时将氮浓度降至最低。结果表明,繁殖 A. niger ITV02 的最佳方法是使用孢子作为接种剂,同时使用纤维素/木质素比率较高的材料(如 CS 和 SB)来生成纤维素酶。这些条件促进了纤维素酶向 β-葡萄糖苷酶生产方向的表达,而不像 BS 和 RH 等纤维素/木质素比率较低的材料表现出较低的纤维素酶活性。经测定,黑曲霉 ITV02 生产纤维素酶的最佳条件为 33 °C、pH 值 5.3 和 200 rpm,从而使外切葡聚糖酶(FP 酶活性)提高了 1.7 倍(从 0.127 U/mL提高到 0.215 U/mL)。这些研究结果表明,利用纤维素/木质素含量高的基质和最佳操作条件,无需提高基础培养基中的氮含量,就能提高 FPase 的活性,从而减轻纤维素酶生产对经济的影响。
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引用次数: 0
Efficacy of two different microbial consortia on salinity tolerance in chickpea: an in-planta evaluation on biochemical, histochemical, and genomic aspects. 两种不同微生物群对鹰嘴豆耐盐性的影响:对种植园内生化、组织化学和基因组方面的评估。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-29 DOI: 10.1007/s13205-024-04124-1
Arumugam Sathya, Vahida Rehman, Vadlamudi Srinivas, Himabindu Kudapa, Subramaniam Gopalakrishnan

This study aimed to identify and characterize actinobacteria and rhizobia with plant growth-promoting (PGP) traits from chickpea plants. Out of 275 isolated bacteria, 25 actinobacteria and 5 chickpea rhizobia showed 1-aminocyclopropane-1-carboxylate deaminase (ACCd) activity. Selected chickpea rhizobia were tested for their nodulating capacity under sterile and non-sterile soil conditions. Further screening on salinity and PGP traits identified three promising isolates: Nocardiopsis alba KG13, Sinorhizobium meliloti KGCR17, and Bacillus safensis KGCR11. These three isolates were analyzed for their compatibility and made into a consortium (Consortium 1). This along with another consortium made from our salinity-tolerant lab strains Chryseobacterium indologenes ICKM4 and Stenotrophomonas maltophilia ICKM15 (Consortium 2) was compared in planta studies. Trials revealed that Consortium 2 showed significant (p < 0.05) tolerance and on above-ground, below-ground traits and yield components than Consortium 1. Moreover, both consortia induced nodulation in saline-stressed plants, alleviated electrolyte leakage (2.3 vs. 0.4 in ICCV 2; 1.8 vs. 0.6 in JG 11), and increased chlorophyll content. Histochemical staining indicated reduced oxidative stress and lipid peroxidation in consortium-treated plants under salinity stress. Further, gene expression studies revealed mixed patterns, with up-regulation of antioxidant and transporter genes observed in consortium-treated plants, particularly in Consortium 2. Overall, Consortium 2 showed better gene expression levels for antioxidant and transporter genes, indicating its superior efficacy in mitigating salinity stress in chickpea plants. This study provides valuable insights into the potential use of these microbial isolates in improving chickpea productivity by enhancing salinity tolerance.

本研究旨在从鹰嘴豆植物中鉴定具有促进植物生长(PGP)特性的放线菌和根瘤菌并确定其特征。在分离出的 275 种细菌中,25 种放线菌和 5 种鹰嘴豆根瘤菌具有 1-氨基环丙烷-1-羧酸脱氨酶(ACCd)活性。对所选鹰嘴豆根瘤菌在无菌和非无菌土壤条件下的结瘤能力进行了测试。对盐度和 PGP 性状的进一步筛选确定了三种有前景的分离株:Nocardiopsis alba KG13、Sinorhizobium meliloti KGCR17 和 Bacillus safensis KGCR11。对这三个分离株的兼容性进行了分析,并将其组成一个联合体(联合体 1)。该联合体与另一个由我们实验室的耐盐碱菌株 Chryseobacterium indologenes ICKM4 和 Stenotrophomonas maltophilia ICKM15 组成的联合体(联合体 2)在植物研究中进行了比较。试验结果表明,联合体 2 显示出显著的(p
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引用次数: 0
Sodium propionate ameliorates lipopolysaccharide-induced acute respiratory distress syndrome in rats via the PI3K/AKT/mTOR signaling pathway. 丙酸钠通过PI3K/AKT/mTOR信号通路改善脂多糖诱发的大鼠急性呼吸窘迫综合征。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-30 DOI: 10.1007/s13205-024-04130-3
Fang He, Jiang-Shan Zhong, Chun-Lan Chen, Peng Tian, Jie Chen, Xian-Ming Fan

Acute respiratory distress syndrome (ARDS) is a severe lung disease characterized by significant hypoxemia, which impairs the oxygen supply necessary for optimal lung function. This study aimed to investigate the effects of sodium propionate (SP), the primary end product of intestinal flora fermentation of dietary fiber, on lipopolysaccharide (LPS)-induced ARDS in rats. The rats were treated with SP, after which the lung wet/dry ratio, arterial partial oxygen pressure (PaO2), levels of pro- and anti-inflammatory cytokines, tight junction proteins ZO-1 and Occludin, as well as LC3 and phosphorylated PI3K (p-PI3K)/p-AKT/p-mTOR protein levels, were measured. Additionally, histopathological analysis was conducted. The results indicated that SP effectively alleviated arterial hypoxemia in rats and mitigated the pathological damage to both intestinal and lung tissues caused by LPS. Notably, SP significantly reduced the levels of inflammatory factors TNF-α and IL-6 in the blood and bronchoalveolar lavage fluid (BALF) of ARDS rats, while increasing the concentration of the anti-inflammatory factor IL-10. Furthermore, SP inhibited the activation of the PI3K/AKT/mTOR signaling pathway and enhanced the LC3II/LC3I ratio in lung tissue. Therefore, SP may improve LPS-induced ARDS in rats by inhibiting the activation of the PI3K/AKT/mTOR signaling pathway, promoting autophagy, decreasing the production and release of inflammatory markers, and reducing alveolar epithelial damage.

急性呼吸窘迫综合征(ARDS)是一种严重的肺部疾病,其特征是显著的低氧血症,会损害最佳肺功能所需的氧气供应。本研究旨在探讨肠道菌群发酵膳食纤维的主要最终产物丙酸钠(SP)对脂多糖(LPS)诱导的大鼠 ARDS 的影响。用SP处理大鼠后,测量肺干湿比、动脉血氧分压(PaO2)、促炎和抗炎细胞因子水平、紧密连接蛋白ZO-1和Occludin以及LC3和磷酸化PI3K(p-PI3K)/p-AKT/p-mTOR蛋白水平。此外,还进行了组织病理学分析。结果表明,SP 能有效缓解大鼠动脉低氧血症,减轻 LPS 对肠道和肺组织造成的病理损伤。值得注意的是,SP能明显降低ARDS大鼠血液和支气管肺泡灌洗液(BALF)中炎症因子TNF-α和IL-6的水平,同时提高抗炎因子IL-10的浓度。此外,SP 还能抑制 PI3K/AKT/mTOR 信号通路的激活,并提高肺组织中 LC3II/LC3I 的比率。因此,SP可通过抑制PI3K/AKT/mTOR信号通路的激活、促进自噬、减少炎症标志物的产生和释放以及减轻肺泡上皮损伤来改善LPS诱导的大鼠ARDS。
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引用次数: 0
Augmentation of antifungal activity of fluconazole using a clove oil nanoemulgel formulation optimized by factorial randomized D-optimal design. 使用丁香油纳米凝胶配方增强氟康唑的抗真菌活性,该配方通过因子随机 D-优化设计进行了优化。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-18 DOI: 10.1007/s13205-024-04116-1
Shaimaa M Badr-Eldin, Hibah Mubarak Aldawsari, Sabna Kotta, Mahmoud Abdelkhalek Elfaky

In the present study, fluconazole (FLU) showed the highest solubility in clove oil and was selected as the oil phase for the FLU-loaded nanoemulsion (FLU-NE). Among the studied cosurfactants, Labrafac was better than ethanol at providing globules with acceptable sizes and a lower polydispersity index (PDI) when Tween 80 was the surfactant. This optimized FLU-NE was thermodynamically stable. Furthermore, FLU-NE stored at 40 ± 2 °C and 75 ± 5% relative humidity for 6 months demonstrated good stability. The FLU-NE was converted to a FLU-loaded nanoemulsion gel (FLU-NEG) using 2% w/v sodium carboxymethyl cellulose. The FLU-NEG was acceptable in terms of visual appearance and spreadability. Rheological studies revealed pseudoplastic behavior for FLU-NEG. The viscosity of FLU-NEG decreased when the applied rpm was increased. FLU-NEG showed greater drug release than that from a FLU-GEL formulation. Furthermore, the FLU release from FLU-NEG followed the Higuchi model. The results from the in vitro antifungal evaluation of FLU-NEG on Candida albicans ATCC 76615 strain confirmed the increase in the antifungal activity of FLU by clove oil. Significant differences were observed in the zones of inhibition produced by FLU-NEG compared to those produced by the blank nanoemulsion gel (B-NEG), fluconazole suspension (FLU-SUS), and nystatin samples. Thus, the increase in the antifungal activity of FLU using clove oil as the oil phase in its nanoemulsion formulation was quite evident from our results. Therefore, the developed FLU-NEG could be considered a potential candidate for further preclinical and clinical studies.

在本研究中,氟康唑(FLU)在丁香油中的溶解度最高,因此被选为氟康唑负载纳米乳液(FLU-NE)的油相。在所研究的共表面活性剂中,当 Tween 80 作为表面活性剂时,Labrafac 在提供具有可接受尺寸和较低多分散指数(PDI)的球状物方面优于乙醇。这种优化的 FLU-NE 具有热力学稳定性。此外,FLU-NE 在 40 ± 2 °C 和 75 ± 5%相对湿度条件下储存 6 个月后显示出良好的稳定性。使用 2% w/v 羧甲基纤维素钠将 FLU-NE 转化为 FLU 负载纳米乳液凝胶(FLU-NEG)。FLU-NEG 在外观和涂抹性方面均可接受。流变学研究表明,FLU-NEG 具有假塑性行为。当转速增加时,FLU-NEG 的粘度降低。与 FLU-GEL 制剂相比,FLU-NEG 的药物释放量更大。此外,FLU-NEG 的 FLU 释放遵循樋口模型。FLU-NEG 对白色念珠菌 ATCC 76615 株的体外抗真菌评价结果证实,丁香油提高了 FLU 的抗真菌活性。与空白纳米乳凝胶(B-NEG)、氟康唑悬浮剂(FLU-SUS)和奈司他丁样品相比,FLU-NEG 产生的抑菌区有显著差异。因此,从我们的研究结果来看,以丁香油作为纳米乳液配方中的油相,FLU 的抗真菌活性明显提高。因此,所开发的 FLU-NEG 可被视为进一步临床前和临床研究的潜在候选药物。
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引用次数: 0
Utilization of Aspergillus niger for the fermentative production of azaphilone dye in YEPB medium. 利用黑曲霉在 YEPB 培养基中发酵生产唑萘酮染料。
IF 2.6 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Pub Date : 2024-11-01 Epub Date: 2024-10-04 DOI: 10.1007/s13205-024-04098-0
Muhammad Usman Ahmad, Abeera Ahmad, Sadaf Mutahir, Muhammad Asim Khan, Sikander Ali, Abdulrahman A Almehizia, Kaynat William

The current research focuses on the production and optimization of a natural yellowish-brown Azaphilone dye using Aspergillus niger. A variety of culture media were tested to ascertain the best conditions for dye synthesis. The formation of the yellowish-brown dye was confirmed by a color shift in the reaction mixture, and UV-Vis spectroscopy detected the dye at 450 nm. Static conditions were found to be more favorable than shaking for higher dye yields, and fed-batch fermentation was more effective than batch fermentation. Maximum dye production was achieved after 28 days of incubation. Factors such as temperature, pH, and inoculum percentage were shown to influence dye synthesis, with the highest production (2.5 ml) occurring at 30 °C, pH 7, and a 3% spore suspension in yeast extract peptone broth (YEPB) medium under static conditions. Gas chromatography-mass spectrometry (GC-MS) analysis validated the presence of Azaphilone dye in the culture filtrate. The dye was successfully applied to a pretreated cotton cloth. These findings advance our understanding of optimizing fungal dye production for sustainable and eco-friendly textile coloration applications. This study appears to be the first of its kind to report azaphilone dye production by A. niger in the YEPB medium.

目前的研究重点是利用黑曲霉生产和优化天然黄棕色 Azaphilone 染料。研究人员测试了多种培养基,以确定染料合成的最佳条件。反应混合物的颜色变化证实了黄棕色染料的形成,紫外可见光谱在 450 纳米波长处检测到了染料。研究发现,静置条件比振荡条件更有利于提高染料产量,而喂料-分批发酵比分批发酵更有效。培养 28 天后染料产量达到最大。温度、pH 值和接种物百分比等因素都会影响染料的合成,在静态条件下,温度为 30 °C、pH 值为 7、酵母提取物蛋白胨肉汤(YEPB)培养基中孢子悬浮液含量为 3% 时,染料产量最高(2.5 毫升)。气相色谱-质谱(GC-MS)分析验证了培养滤液中存在 Azaphilone 染料。该染料被成功应用于预处理过的棉布上。这些发现加深了我们对优化真菌染料生产以实现可持续和生态友好型纺织品着色应用的理解。这项研究似乎是首次报道黑僵菌在 YEPB 培养基中生产萘甲萘醌染料。
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3 Biotech
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