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The differences in inflammation changes between lymphoid and non lymphoid tissues of mice after i.p. E.coli injection and its effect on renal function 腹腔注射大肠杆菌后小鼠淋巴组织与非淋巴组织炎症变化的差异及对肾功能的影响
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61039
Saad A. AL-Jashaami, Noori M. Lauibi, J. Jouda
Splenic microenvironments are suitable for the definition of immunologic processes, which may different than other non lymphoid organs. Splenic changes have been identified after Leishmania and viral infections, but after bacterial need more study. In kidney, as a non lymphoid organ, the bacterial infection associated with acute kidney injury. Acute kidney injury can lead to chronic kidney disease which can lead to kidney failure. The present work amid to study the differences between bacterial infection stages in lymphoid organ, spleen, and nonlymphoid organ, kidney; and the effect of bacterial infection stage on the renal function. 20 males Balb/c mice were used in this study, aged about 2 months and weight about 24g. 15 mice were injected intraperitoneal with 200μl E.coli, isolated from children have urinary infection, in final concentration (10cell /ml), and 5 mice were injected with only normal saline as control group. The mice were killed in different time after E.coli injection: 24, 48, and 72h. Blood was collected and used in serum creatinine and blood urea determinations. Spleen and kidney were collected, fixed in 10% formalin solution, and processed by standard procedures. The body weights were recorded before sacrifice. The results showed that: significantly higher body weight in infected groups within 24, 48, and 72h compared to zero time and control group, significantly negative correlations between body weight and infection duration, white pulp widening, back off red pulp 10 Saad A. AL-Jashaami et al. size, and activation of germinal center with the time after bacterial injection, infiltration of inflammatory cells and appearing of the focal necrosis of renal epithelial tubules within 48h after bacterial infection and increased after 72h, significantly higher serum creatinine (S.Cr) and blood urea (B.Urea) in the infected groups within 48 and 72h after bacterial injection compared to control group, and significantly positive correlations between S.Cr and B.Urea levels in infected groups and all groups together but not in the control group.
脾微环境适合于免疫过程的定义,这可能不同于其他非淋巴器官。利什曼原虫和病毒感染后已发现脾脏改变,但细菌感染后需要更多的研究。肾脏作为非淋巴器官,细菌感染可引起急性肾损伤。急性肾损伤可导致慢性肾病,慢性肾病可导致肾衰竭。本研究旨在探讨细菌感染在淋巴器官、脾脏和非淋巴器官、肾脏的分期差异;以及细菌感染阶段对肾功能的影响。本研究选用雄性Balb/c小鼠20只,年龄约2月龄,体重约24g。15只小鼠腹腔注射终浓度为200μl(10细胞/ml)的尿路感染患儿分离大肠杆菌,5只小鼠腹腔注射生理盐水作为对照组。注射大肠杆菌后,小鼠分别在24、48、72h的不同时间死亡。采集血液用于测定血清肌酐和血尿素。取脾、肾,用10%福尔马林溶液固定,按标准程序处理。献祭前记录体重。结果表明:感染组在24、48和72h内的体重明显高于零时间组和对照组,体重与感染持续时间、白牙髓扩大、退红牙髓10 Saad A. AL-Jashaami等人的大小、生发中心的激活与注射细菌后的时间呈显著负相关。感染组在细菌注射后48h和72h内血清肌酐(S.Cr)和血尿素(B.Urea)均显著高于对照组,感染组和各组间S.Cr和B.Urea水平呈显著正相关,对照组间无显著正相关。
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引用次数: 0
Dynamic modeling process from the hexane removal of contaminated air bio filtration method 动态建模过程从正己烷去除污染空气的生物过滤方法
Pub Date : 2017-01-01 DOI: 10.12988/asb.2017.724
M. K. Asl, M. Sohrabi, S. Yaghoubi
Purification of polluted air from volatile organic compounds by means of biologic methods has been considered due to great advantages. Biofiltration is one of the best biologic methods for doing such processes. In this research, a mathematical model has been presented in order for Biofiltration process of polluted air in which limiter effects of oxygen and humidity have been considered. Since, microbe’s inclination to substrate and microbial growth and production output of biomass are effective on bio filter’s performance, evaluating the effect of these quantities is necessary. Dynamic modeling of Biofiltration process of polluted air in stable and unstable conditions was conducted by emphasizing on humidity in the equations of mass transfer and sensitivity analysis in order for determining change effect of this parameter on the performance of model. The results show that the maximum amount of special growth results in the increase of absorption capacity, and also the model rightly confirms that constant increase of saturation 114 Morteza Kashefi Asl et al. reduces the absorption capacity; whilst by means of this model, we can show that special surface switch and thickness change of biofilm are effective on absorption capacity.
利用生物方法净化污染空气中的挥发性有机化合物具有很大的优势。生物过滤是进行此类处理的最佳生物方法之一。本文建立了考虑氧气和湿度限制作用的污染空气生物过滤过程的数学模型。由于微生物对基质的倾斜度、微生物的生长和生物量的产量对生物过滤器的性能是有效的,因此评估这些数量的影响是必要的。对稳定和不稳定条件下污染空气的生物过滤过程进行了动态建模,在传质方程和灵敏度分析中重点考虑了湿度,以确定该参数的变化对模型性能的影响。结果表明,最大的特殊生长量会导致吸收容量的增加,模型也正确地证实了饱和度的不断增加会降低吸收容量。同时,通过该模型可以看出,特殊的表面切换和生物膜厚度的变化对吸收能力是有效的。
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引用次数: 0
Evaluation of the serum lipid profile and dietary intake in patients undergoing cholecystectomy 胆囊切除术患者血脂和饮食摄入的评价
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61140
R. Goodarzi, A. Saedisomeolia, E. F. Moghadam, Ali Sadoogh-Abbasi, A. Sianaki, Zahra Seaf
Introduction: New findings suggest that gallbladder not only plays a role in control of concentration and storage of the bile acids, but also, may be effective on nutrients metabolism, in particular on fat metabolism. Objective: This study aimed to determine the effects of cholecystectomy on serum lipid parameters. Materials and methods: This cross-sectional study was conducted on seventy patients who were undergoing cholecystectomy. The serum concentration of lipoproteins, weight, BMI, and dietary intake of subjects were assessed before and one month after the cholecystectomy operation. Results: No significant difference was found regarding serum concentration of triglycerides, total cholesterol, low-density lipoprotein (LDL), and high-density lipoprotein (HDL) to LDL proportion, while, HDL concentration represented a significant reduction after the cholecystectomy. No significant difference was observed in terms of the dietary intake of energy, fat, protein, carbohydrate, fiber, saturated and unsaturated fat before and after the surgery. Conclusion: We showed that cholecystectomy decreases the HDL-C by the day of 30 after surgery, however, other biomarkers such as lipoproteins, weight, and BMI, did not differ significantly.Besides, the stability of the serum levels of lipoproteins preand post-cholecystectomy may be due to the insignificant changes in factors related to diet.
新的研究结果表明,胆囊不仅控制胆汁酸的浓度和储存,而且可能对营养物质的代谢,特别是脂肪的代谢有效。目的:探讨胆囊切除术对血脂指标的影响。材料与方法:对70例胆囊切除术患者进行横断面研究。在胆囊切除术前和术后1个月评估受试者的血清脂蛋白浓度、体重、BMI和饮食摄入量。结果:两组血清甘油三酯浓度、总胆固醇、低密度脂蛋白(LDL)、高密度脂蛋白(HDL)与低密度脂蛋白(LDL)之比无显著差异,而胆囊切除术后HDL浓度明显降低。术前、术后饮食中能量、脂肪、蛋白质、碳水化合物、纤维、饱和脂肪和不饱和脂肪的摄入量均无显著差异。结论:我们发现胆囊切除术降低了手术后30天的HDL-C,然而,其他生物标志物,如脂蛋白、体重和BMI,没有显著差异。此外,胆囊切除术前后血清脂蛋白水平的稳定可能与饮食相关因素的变化不显著有关。
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引用次数: 1
Smoking model with cravings to smoke 有吸烟欲望的吸烟模型
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61245
A. Awan, Attia Sharif, T. Hussain, M. Ozair
Among smokers, often the desire to quit smoking arise. A large number of smokers attempt to quit, but only a fraction of them are successful. While the health and monetary benefits are inarguable. The huge amount of stress associated with smoking, a combination of powerful cravings, sleep and mood disturbances and cognitive changes often stymie even the most motivated of individuals. In this paper, a mathematical model is proposed and analyzed in which the temporary quitters turn back to smoking by powerful cravings for smoking. We have discussed the qualitative behavior of the model and numerical simulations are carried out to support the analytical results.
在吸烟者中,往往会产生戒烟的愿望。许多吸烟者试图戒烟,但只有一小部分人成功了。虽然健康和金钱上的好处是无可争辩的。吸烟带来的巨大压力、强烈的烟瘾、睡眠和情绪障碍以及认知变化,往往会阻碍最积极的人。本文提出并分析了暂时戒烟者因强烈的吸烟欲望而重新吸烟的数学模型。我们讨论了模型的定性行为,并进行了数值模拟来支持分析结果。
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引用次数: 10
Expression alternations of CCR5 in new case and treated patients with multiple sclerosis CCR5在多发性硬化症新发和治疗患者中的表达变化
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.7411
Shima Bayat, M. Ataei, S. Nabavi, Mojgan Ghahari, M. Sanati
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引用次数: 0
Biomass density and carbon stock of tropical community forest area at FMU Lawu Manunggal-Panekan-Magetan district FMU Lawu Manunggal-Panekan-Magetan地区热带群落林区的生物量密度和碳储量
Pub Date : 2017-01-01 DOI: 10.12988/asb.2017.7924
Ahadiati Rohmatiah, Lyya Supriono, M. Lukito
Forest is a natural element that can absorb CO2 in large quantities. This is due to the existing vegetation in the forests bind CO2 while the process of photosynthesis and store it in the form of biomass. So that, the existence of forests affect the prevention and handling of global warming on earth. This research aims to measure the biomass density and carbon stock of tropical community forests area at Sumberdodol and Tapak Villages of FMU (Forest Managemen Unit) Lawu Manunggal – Panekan – Magetan District. The research method for inventory of individual trees in forest stands uses finger circle plot of 0.05 Ha. Its intensity is 5%. Destructive Biomass Measurements while analyzing carbon stock uses titration method of Wallky and Black. Result of research Biomass density of forests are Melia 61,288,649 kg/Ha, Mahogany 97,472,875 kg/Ha, Teak 88,925,376 kg/Ha, and Albicia 25,566,573 kg/ Ha. FMU Lawu Manunggal forest 206 Ahadiati Rohmatiah et al. area at Sumberdodol and Tapak is 276,33 Ha. Total Biomass density of Melia 1,225.77 tons, Mahogany 2,216.47 tons, Teak 1,778.51 tons, and albicia 511.33 tons. Total forest stands are 5,732.08 tons. The carbon stock average of Melia 28,806 ton/Ha, Mahogany 45,812 ton/Ha, Teak type 41,795 ton/Ha, and Albicia 12,016 ton/Ha. The total Carbon stock of Sumberdodol and Tapak Villages are 2,694.08 tons.
森林是一种可以大量吸收二氧化碳的自然元素。这是由于森林中现有的植被在光合作用过程中结合二氧化碳并以生物量的形式储存起来。因此,森林的存在影响着地球上全球变暖的预防和处理。本研究旨在测量森林管理单位(FMU) Lawu Manunggal - Panekan - Magetan地区sumberdodool村和Tapak村热带群落森林的生物量密度和碳储量。林分单株清查的研究方法采用0.05 Ha手指圈样地。其强度为5%。破坏性生物量测量,同时分析碳储量使用沃基和布莱克滴定法。研究结果森林生物量密度为木质素61,288,649 kg/Ha,红木97,472,875 kg/Ha,柚木88,925,376 kg/Ha,白檀木25,566,573 kg/Ha。FMU Lawu Manunggal森林206 Ahadiati Rohmatiah等在Sumberdodol和Tapak的面积为276,33公顷。总生物量密度为梅利亚1225.77吨、红木2216.47吨、柚木1778.51吨、白灰511.33吨。森林蓄积量5732.08吨。黑茉莉平均碳储量28806吨/公顷,红木平均碳储量45812吨/公顷,柚木平均碳储量41795吨/公顷,白檀木平均碳储量12016吨/公顷。松都都村和塔巴克村的总碳储量为2694.08吨。
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引用次数: 0
The assessment of neuronal density of CA1 and CA2 regions in hippocampus of newborn rats born from mothers fed by Cantharellus cibarius hidroalcoholic extract 大鼠海马CA1和CA2区神经元密度的变化
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.7521
Z. Esmaili, M. Tehranipour
One of the characteristics of human is the ability to change behavior based on previous experience and learning is the gaining the information that makes it possible. Memory is somewhere the preserve and storing this information. The hippocampus is one of centers in the brain responsible for learning and this center is a part of limbic system where new neurons are generated through neurogenesis. The aim of this study was to assess the changes in neuronal density in CA1 and CA2 regions in hippocampus of rats born from mothers fed by Cantharellus cibarius extract. In this experimental study, 24 female Wistar rats weighing 200 250 g were randomly divided to randomize controlled and 1, 2 and 3 treated groups with Cantharellus cibarius hydroalcoholic extract with doses of 100, 250 and 500. Treatment was performed through gavage method 7 days before mating. After delivery, one newborn rat of each mother was accidentally selected and anesthetized with ether, and after removing the skull, tissue processing was per182 Zahra Esmaili and Maryam Tehranipour formed in order to study the neuronal density in CA1 and CA2 areas by stereology and dissector methods, and all findings were compared by using ANOVA and t-test at significance level, (p<0.05).In control and 1, 2 and 3 experimental groups, the mean of neuronal density of CA1 was 2423 ±1.59, 3425±183, 4003 ± 101 and 4822 ±19.5, respectively, in CA2 area it was 2066±390, 3568±150, 3993 ±65.05 and 4629 ±64.7. Statistical analysis showed significant increase in neuronal density in (CA1, CA2) areas in experimental group (doses of 100, 250 and 500 mg / kg hydroalcoholic extract) compared to the control group, (p<0.05). It is possible that the Cantharellus cibarius extract increases the neurogenesis by excess neuronal density due to minerals such as magnesium, calcium, zinc, vitamin D and vitamin E.
人类的特征之一是根据以前的经验改变行为的能力,学习是获得使之成为可能的信息。记忆是保存和存储这些信息的地方。海马体是大脑中负责学习的中心之一,该中心是边缘系统的一部分,新神经元通过神经发生产生。本研究的目的是评估大鼠海马CA1和CA2区神经元密度的变化。实验选取体重200 ~ 250 g的雌性Wistar大鼠24只,随机分为对照组和1、2、3组,分别给予100、250、500剂量的红斑马草水酒精提取物。配种前7天采用灌胃法处理。分娩后,每只母鼠意外选取1只新生大鼠,用乙醛麻醉,取颅骨后,组织处理perzahra Esmaili和Maryam Tehranipour,通过立体学和解剖学方法研究CA1和CA2区神经元密度,所有结果采用方差分析和t检验,在显著性水平上,(p<0.05)。对照组和1、2、3实验组CA1区神经元密度均值分别为2423±1.59、3425±183、4003±101和4822±19.5,CA2区神经元密度均值分别为2066±390、3568±150、3993±65.05和4629±64.7。统计分析显示,实验组(100、250和500 mg / kg水酒精提取物剂量)与对照组相比,(CA1、CA2)区神经元密度显著增加(p<0.05)。这可能是由于斑蝥素提取物中含有镁、钙、锌、维生素D和维生素E等矿物质,使神经元密度增加,从而增加了神经发生。
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引用次数: 0
Effects of different burst form on gene expression dynamic and first-passage time 不同爆发形式对基因表达动态和首次传代时间的影响
Pub Date : 2017-01-01 DOI: 10.12988/asb.2017.726
Xuejie Liu, Qiuying Li
Timing of events in many cellular processes, such as cell-cycle control, cell differentiation, and so on, depend on regulatory proteins reaching critical threshold levels. The time at which special protein cross up the threshold levels is called as the first-passage time(FPT). Here, we theoretically show that geometric conditional burst has unneglectable effects on gene expression and FPT. More precisely, if the mean of protein is fixed and the same burst size, conditional geometric burst increase the mean and noise of FPT compared with the geometric distribution. If the mean of FPT is fixed and the same burst size, conditional geometric burst increase noise of FPT compared with the geometric distribution.
在许多细胞过程中,如细胞周期控制、细胞分化等,事件的定时取决于调节蛋白达到临界阈值水平。特殊蛋白质越过阈值水平的时间称为首次通过时间(FPT)。在这里,我们从理论上证明了几何条件爆发对基因表达和FPT有不可忽视的影响。更确切地说,当蛋白质的均值固定且爆发大小相同时,条件几何爆发比几何分布增加了FPT的均值和噪声。当FPT均值固定且突发大小相同时,条件几何突发比几何分布增加了FPT的噪声。
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引用次数: 0
The effect of Cantharellus Cibarius hidroalcoholic extract on fertility and sex determination in rats 斑蝥油醇提取物对大鼠生育能力和性别决定的影响
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61244
A. Karimi, M. Tehranipour, Mahmud Zokaei
Having a healthy child is the dream of every couple, and the child's gender for many couples is very important. The effect of nutrition on gender in several studies evaluated and is one of the most popular methods which today, as the common form is recommended for embryo selection. The aim of this study was to investigate Cantharellus Cibarius hydroalcoholic extracts on fertility and sexing in rats. Twenty four wistar rats(female)with weighing approximately 200-250 grams were randomly divided into four groups, including control and treatment groups with the above mentioned mushroom hydroalcoholic extract (with doses of 100, 250 and 500 mg/ kg). The Cantharellus Cibarius extract were fed to rats in by gavage method seven consecutive days and then each rat in a separate cage in adjacent to the male of the same breed were mated, after 19-21 days of pregnancy, newborns being counting and sexing. Quantitative data analysis using the software minitab and by ANOVA and t-test was performed. According to the findings, the number of births in the treatment groups compared to the control group 54 Asiye Karimi et al. there is no significant difference, but in the sexing, the female set in treatment groups compared to the control group showed a significant difference (p<0.05) that increase in the treated groups with doses of 500 was higher. As a result, increase of gynogenesis in treated groups with the extract of the mushroom Cantharellus Cibarius is probably due to calcium and magnesium ions.
拥有一个健康的孩子是每对夫妇的梦想,孩子的性别对许多夫妇来说是非常重要的。营养对性别的影响在几项研究中被评估,并且是当今最流行的方法之一,作为胚胎选择的常用形式被推荐。本研究旨在探讨红车前草水醇提取物对大鼠生殖能力和性别的影响。选取体重约200-250克的wistar雌性大鼠24只,随机分为对照组和处理组(剂量分别为100、250和500 mg/ kg)。用灌胃法连续喂大鼠7 d,在妊娠19 ~ 21 d后,与同一品种的雄鼠相邻,每只大鼠单独饲养,进行交配,对新生鼠进行计数和性别鉴定。采用minitab软件对数据进行定量分析,采用方差分析和t检验。根据研究结果,54 Asiye Karimi等治疗组的出生数与对照组相比无显著差异,但在性别上,治疗组的雌性集与对照组相比有显著差异(p<0.05),剂量为500的治疗组增加幅度更高。结果表明,加香菇提取物对雌蕊发生的促进作用可能与钙、镁离子有关。
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引用次数: 1
Pharmacochemistry of Iranian flora Artemisia oliveriana and its antimalarial effects on Plasmodium berghei in vivo 伊朗植物艾草的体内药物化学及其对伯氏疟原虫的抗疟作用
Pub Date : 2017-01-01 DOI: 10.12988/ASB.2017.61141
Touraj Miandoabi, K. H. Tappeh, H. Nahrevanian, Seyed Masoud Kazemi, H. Mohammadzadeh, Haleh Hanifian
The aim of this study was to evaluate antimalarial effects of Iranian flora Artemisia oliveriana on Plasmodium berghei in vivo and photochemistry of its natural effective components. This is the first application of Iranian flora A. oliveriana on murine malaria in Iran. The aerial parts of A. oliveriana were collected at the flowering stage from Qum and Isfahan provinces, in central part of Iran in 2010. The aerial parts were air-dried at room temperature and then powdered and macerated in methanol and filtered and it was extracted and dried by Rotary Evaporator. The toxicity of herbal extract was assessed on four groups of naive NMRI mice and it‘s antimalarial efficacy was investigated on infected P. berghei animals. The significance of differences was determined by student‘s ttest using Graph Pad prism software. The results indicated no toxicity was observed even by high concentration of herbal extract by measuring body weight, survival rate and hepato/ splenomegaly. It is demonstrated that total extract A. oliveriana possesses therapeutic inactivity against P. berghei which indicates its antimalarial effects in vivo on NMRI mice. 44 Touraj Miandoabi et al.
本研究旨在评价伊朗植物橄榄蒿(Artemisia oliveriana)体内抗伯氏疟原虫(Plasmodium berghei)的作用及其天然有效成分的光化学性质。这是伊朗植物A. oliveriana在伊朗首次应用于小鼠疟疾。本研究于2010年在伊朗中部库姆省和伊斯法罕省采集花期空中部分。空气部件在室温下风干,然后在甲醇中粉碎浸渍,过滤后用旋转蒸发器提取干燥。研究了中药提取物对四组NMRI小鼠的毒性作用,并对感染伯氏疟原虫的动物进行了抗疟作用的研究。采用Graph Pad prism软件进行学生检验,确定差异的显著性。结果表明,即使是高浓度的草药提取物也没有毒性,通过测量体重、存活率和肝/脾肿大。结果表明,榄香总提取物对柏氏疟原虫无治疗作用,表明其在NMRI小鼠体内具有抗疟作用。[4]图拉杰。
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引用次数: 0
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Advanced Studies in Biology
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