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Additive manufacturing of personalized scaffolds for vascular cell studies in large arteries: A case study on carotid arteries in sickle cell disease patients 用于大动脉血管细胞研究的个性化支架的增材制造:镰状细胞病患者颈动脉案例研究
Q3 Medicine Pub Date : 2024-10-05 DOI: 10.1016/j.stlm.2024.100178
Saskia Eckert , Christian Kassasseya , Weiqiang Liu , Eliott Benichou , Irène Vignon-Clementel , Smaïne Kouidri , Kim-Anh Nguyen-Peyre , Pablo Bartolucci , Frédéric Segonds
Patient-specific models have increasingly gained significance in medical and research domains. In the context of hemodynamic studies, computational fluid dynamics emerges as a highly innovative and promising approach. We propose to augment these computational studies with cell-based experiments in individualized artery geometries using personalized scaffolds and vascular cell experiments. Previous research has demonstrated that the development of Sickle Cell Disease (SCD)-Related Vasculopathy is dependent on personal geometries and flow characteristics of the carotid artery. This fact leaves conventional animal experiments unsuitable for gaining patient-specific insights into cellular signaling, as they cannot replicate the personalized geometry. These personalized dynamics of cellular signaling may further impact disease progression, yet remains unclear. This paper presents a six-step methodology for creating personalized large artery scaffolds, focusing on high-precision models that yield biologically interpretable patient-specific results. The methodology outlines the creation of personalized large artery models via Additive Manufacturing suitably for supporting cell culture and other cellular experiments. Additionally, it discusses how different Computer-Aided-Design (CAD) construction modes can be used to obtain high-precision personalized models, while simplifying model reconfigurations and facilitating adjustments to general designs such as system connections to bioreactors, fluidic systems and visualization tools. A proposal for quality control measures to ensure geometric congruence for biological relevance of the results is added. This innovative, interdisciplinary approach appears promising for gaining patient-specific insights into pathophysiology, highlighting the importance of personalized medicine for understanding complex diseases.
患者特异性模型在医疗和研究领域的重要性日益凸显。在血液动力学研究中,计算流体动力学是一种极具创新性且前景广阔的方法。我们建议利用个性化支架和血管细胞实验,在个性化动脉几何结构中进行基于细胞的实验,以增强这些计算研究。先前的研究表明,镰状细胞病(SCD)相关血管病变的发展取决于颈动脉的个性化几何形状和流动特性。由于无法复制个性化的几何形状,传统的动物实验无法深入了解患者的细胞信号传导。这些个性化的细胞信号动态可能会进一步影响疾病的进展,但目前仍不清楚。本文介绍了创建个性化大动脉支架的六步方法,重点是建立高精度模型,以产生可从生物学角度解释的患者特异性结果。该方法概述了通过快速成型制造技术创建个性化大动脉模型,以支持细胞培养和其他细胞实验。此外,它还讨论了如何利用不同的计算机辅助设计(CAD)构建模式来获得高精度的个性化模型,同时简化模型的重新配置并促进对一般设计的调整,如与生物反应器、流体系统和可视化工具的系统连接。此外,还提出了质量控制措施建议,以确保结果的几何一致性和生物学相关性。这种创新的跨学科方法似乎很有希望获得针对病人的病理生理学见解,突出了个性化医学对理解复杂疾病的重要性。
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引用次数: 0
3D printable customized drug delivery system for the vaginal delivery of therapeutics: Unlocking potential prospects for women care 用于阴道给药的 3D 可打印定制给药系统:打开妇女护理的潜在前景
Q3 Medicine Pub Date : 2024-10-05 DOI: 10.1016/j.stlm.2024.100177
Suraj Kumar , Rishabha Malviya , Sathvik Belagodu Sridhar
The current traditional medication administration method is characterized by a prevalent "one-size-fits-all" approach poses challenges in tailoring medications to individual pharmacokinetic profiles, limiting its applicability in the pharmaceutical sector. In response to this, three-dimensional (3D) printable medical devices for vaginal drug delivery are gaining popularity, due to advancements in Fused Deposition Modeling (FDM) or additive manufacturing (AM) technology. These devices offer distinct Strengths over conventional medication delivery methods, enabling the customization of drugs and the creation of intricate three-dimensional structures with personalized designs. In an additional point of interest, the development of multi-material printing integrates the beneficial properties of a variety of functional substances, presenting diverse opportunities to enhance the efficacy of vaginal drug delivery. Although there have been substantial advancements in manufacturing processes, challenges such as cost-effectiveness, scalability, and compliance with regulations remain substantial hurdles. This review focuses on the recent progress made in the field of printed using three-dimensional technology for vaginal medication delivery devices, addresses the primary challenges that need to be overcome, and explores the potential of manufacturing techniques in shaping the future of dosage forms and drug administration systems.
当前传统给药方法的特点是普遍采用 "一刀切 "的方法,这给根据个体药代动力学特征定制药物带来了挑战,限制了其在制药领域的适用性。为此,随着熔融沉积建模(FDM)或增材制造(AM)技术的发展,用于阴道给药的三维(3D)可打印医疗器械越来越受欢迎。与传统的给药方法相比,这些设备具有明显的优势,可以实现药物的定制,并创造出具有个性化设计的复杂三维结构。另一个值得关注的问题是,多材料打印技术的发展整合了多种功能物质的有益特性,为提高阴道给药的疗效提供了多种机会。虽然制造工艺有了长足的进步,但成本效益、可扩展性和法规遵从性等挑战仍然是巨大的障碍。本综述重点介绍了利用三维技术印制阴道给药装置领域的最新进展,探讨了需要克服的主要挑战,并探讨了制造技术在塑造未来剂型和给药系统方面的潜力。
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引用次数: 0
A novel approach to hallucal sesamoid pathology utilizing a 3D printed patient specific total sesamoid replacement implant: Case series 利用 3D 打印的患者专用全芝麻状替代假体治疗幻觉芝麻状病变的新方法:病例系列
Q3 Medicine Pub Date : 2024-09-14 DOI: 10.1016/j.stlm.2024.100174
Tyler R. Rodericks , Adam D. Perler , Hans C. Humrick

Background

Sesamoidectomy can be associated with multiple complications secondary to loss of the intrinsic function of the excised sesamoid. We sought to mitigate these complications by preserving sesamoid function with a total sesamoid replacement (TSR) in lieu of sesamoidectomy.

Method

Patient specific 3D printed TSR implants were designed and implanted for three patients who had exhaustively failed conservative measures. Follow up ranges from 7-36 months during which we evaluated for complications, symptom resolution, and patient satisfaction.

Result

All patients had complete resolution of pain between 3.5-12 months postop and have remained satisfied with their outcome. No evidence of the aforementioned complications was observed.

Conclusion

TSR may represent a viable alternative treatment option for most cases in which sesamoidectomy would otherwise be considered.

Level of evidence

IV, case series.

背景芝麻状骨切除术可能会继发多种并发症,因为切除的芝麻状骨会丧失固有功能。我们试图用全芝麻假体置换术(TSR)来代替芝麻切除术,以保留芝麻功能,从而减少这些并发症的发生。结果所有患者在术后 3.5-12 个月内疼痛完全缓解,并对治疗效果表示满意。结果所有患者在术后3.5-12个月期间疼痛完全缓解,并对疗效表示满意,没有发现上述并发症。
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引用次数: 0
Selective laser sintering at the Point-of-Care 3D printing laboratory in hospitals for cranio-maxillo-facial surgery: A further step into industrial additive manufacturing made available to clinicians 用于颅颌面外科手术的医院护理点 3D 打印实验室的选择性激光烧结技术:向临床医生提供工业增材制造技术又迈进了一步
Q3 Medicine Pub Date : 2024-09-12 DOI: 10.1016/j.stlm.2024.100175
Alessandro Tel , Erik Kornfellner , Enikő Molnár , Shiden Johannes , Francesco Moscato , Massimo Robiony

Additive manufacturing has developed rapidly in recent years and has many useful applications in the clinical field. In particular, cranio-maxillo-facial (CMF) surgery requires high precision, which can be obtained with 3D printed patient-specific surgical guides and anatomical models. Among the many different printing options, selective laser sintering (SLS) seems to be rarely used in point-of-care applications, considering its apparent characteristics.

This article examines the advantages and disadvantages of SLS printers for CMF point-of-care (PoC) by reviewing the literature and comparing in-house printed SLS and stereolithography (SLA) prints.

The investigation showed that the easily sterilizable and robust materials processed by SLS printing are well suited for CMF surgical guides and have clear advantages over SLA parts.

Some barriers to the use of SLS printers in PoC are likely to be the slightly higher complexity and cost.

However, these will decrease as 3D printing technology advances and surgeon acceptance increases, making SLS a practical PoC tool.

快速成型制造技术近年来发展迅速,在临床领域有许多有用的应用。尤其是颅颌面(CMF)手术需要高精度,而这可以通过三维打印的患者专用手术导板和解剖模型来实现。在众多不同的打印选项中,考虑到选择性激光烧结(SLS)的明显特点,它似乎很少用于护理点应用。本文通过回顾文献并比较内部打印的 SLS 和立体光刻(SLA)打印件,研究了用于 CMF 护理点(PoC)的 SLS 打印机的优缺点。调查显示,SLS 打印加工的材料易于消毒且坚固耐用,非常适合用于 CMF 手术导板,与 SLA 零件相比具有明显的优势。
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引用次数: 0
Evaluation of a pilot regional neurotrauma workshop using 3D printed simulation models 使用 3D 打印模拟模型对区域神经创伤试点研讨会进行评估
Q3 Medicine Pub Date : 2024-08-01 DOI: 10.1016/j.stlm.2024.100169
Nalinda Dissanayaka , Hamish Alexander , Michael Redmond , Patrick Pearce , Andrew Hobbins-King , Kate Lee , Danilo Carluccio , Roozbeh Fakhr , Luigi-Jules Vandi , James I. Novak

Objective

Non-neurosurgeons in regional and rural hospitals may be required to operate on patients presenting with a traumatic brain injury where timely transfer to a tertiary hospital is not possible. Confidence and experience can vary significantly due to limited access to hands-on training. Increasing availability to advanced 3D printed models opens new opportunities to provide accurate head models suitable for this purpose. This study evaluated the experience of regional clinicians and nurses following a neurotrauma workshop where 3D printed head models were used to provide training in burr hole and craniotomy procedures.

Methods

A neurotrauma seminar and workshop was hosted at the Sunshine Coast Health Institute, in the state of Queensland, Australia. The workshop component allowed 26 local clinicians and nurses to gain hands-on experience with a 3D printed head model, guided by neurosurgeons from the closest tertiary hospital. Following training, participants completed a short survey.

Results

Prior to this workshop, most participants had never performed a burr hole (58 %, n=15) or interacted with a 3D printed model (69 %, n=18). Overall, most participants indicated that the 3D printed model performed better (58 %, n=15) and much better (15 %, n=4) than their expectations. 81 % (n=21) left the workshop with improved confidence in performing burr hole and craniotomy procedures. Despite some melting of the plastic, 96 % (n=25) of participants would recommend this model to their colleagues.

目的地区和农村医院的非神经外科医生可能需要为脑外伤患者进行手术,而这些患者不可能及时转到三级医院。由于获得实践培训的机会有限,他们的信心和经验会有很大差异。先进 3D 打印模型的日益普及为提供适用于这一目的的精确头部模型带来了新的机遇。本研究评估了地区临床医生和护士在神经创伤研讨会后的经验,在研讨会上,3D 打印的头部模型被用来提供毛刺孔和开颅手术的培训。方法在澳大利亚昆士兰州的阳光海岸卫生研究所举办了神经创伤研讨会和讲习班。研讨会上,26 名当地临床医生和护士在最近的三甲医院神经外科医生的指导下,亲身体验了 3D 打印头部模型。培训结束后,参与者填写了一份简短的调查问卷。结果在参加此次研讨会之前,大多数参与者从未进行过毛刺孔操作(58%,人数=15)或与 3D 打印模型进行过互动(69%,人数=18)。总体而言,大多数参与者表示 3D 打印模型的性能比他们的预期要好(58%,人数=15)或好得多(15%,人数=4)。81% 的学员(21 人)在离开讲习班时表示,他们对实施毛刺孔和开颅手术的信心有所增强。尽管塑料有些融化,但仍有 96% (人数=25)的学员会向同事推荐该模型。
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引用次数: 0
Design and Investigation of a low-cost elbow rehabilitation tool 低成本肘部康复工具的设计与研究
Q3 Medicine Pub Date : 2024-08-01 DOI: 10.1016/j.stlm.2024.100167
Huda Farooq Jameel, Aws Alazawi, Amal Ibrahim Mahmood

Electronic medical development focuses on creating an efficient rehabilitation device that will strengthen all surrounding muscles and enhance elbow performance. The elbow rehabilitation tool (ERT) provides sophisticated methods like exercise and motion analysis. The initiative is notable for its advanced assessment methods and adaptable training curricula, which offer users a thorough and successful therapeutic experience. The ERT includes elements like a stepper motor, variable resistor, steel wire, microcontroller, motor driver, and components created using a 3D printer. The experiment results show that the average systematic error percentage is about 82.857%, where seven healthy people have tested the ERT aged between 22 and 55 (five males and two females). The ERT also has achievement evaluation, which improves motivation and dedication to the recovery methods through an effective rehabilitation experience for users.

电子医疗开发的重点是创造一种高效的康复设备,以增强所有周围肌肉的力量,提高肘部的性能。肘部康复工具(ERT)提供运动和运动分析等先进方法。其先进的评估方法和适应性强的训练课程为用户提供了全面、成功的治疗体验。ERT 包括步进电机、可变电阻器、钢丝、微控制器、电机驱动器和使用 3D 打印机制作的组件。实验结果表明,平均系统误差百分比约为 82.857%,7 名年龄在 22 至 55 岁之间的健康人(5 名男性和 2 名女性)测试了 ERT。ERT 还进行了成就评估,通过为用户提供有效的康复体验,提高了他们对康复方法的积极性和专注度。
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引用次数: 0
3D-printed β-TCP/Ti6Al4V composite scaffolds for bone regeneration: Process parameter optimization and evaluation 用于骨再生的三维打印β-TCP/Ti6Al4V复合支架:工艺参数优化与评估
Q3 Medicine Pub Date : 2024-08-01 DOI: 10.1016/j.stlm.2024.100168
Belgin Paul D L, Praveen Ayyappan Susila, D. Rajamani, Hushein R

A β-TCP/Ti6Al4V composite scaffold with interconnected macro porous architecture was fabricated using Direct Ink Writing (DIW). Pluronic F-127 and de-ionized water was used as binder and solvent for ink preparation. The present work was carried out to study the rheological behavior of the composite bioceramic ink and to investigate DIW process parameters such as Ti6Al4V proportion, infill percentage and extrusion pressure. The Box-Behnken response surface methodology, ANOVA, sensitivity, desirability approach are used for the experimental, statistical and numerical optimization of the parameters suitable for DIW. The output responses such as dimensional error of the fabricated scaffold from the original dimensions and compressive strength are considered for multi-objective optimization. The result defined that the optimal values are solid loading 55 %v/v (40 %v/v of β-TCP, 15 %v/v of Ti6Al4V) and 45 %v/v of Pluronic gel, 98 % infill rate and 6.36 bar pressure. The dimensional error and compressive strength of the scaffold printed at the optimized conditions are found as 1.88 % and 19 MPa with macro and micro pores suitable for bone regeneration with satisfactory biocompatibility assed via MTT assay.

利用直接墨水写入(DIW)技术制造了具有相互连接的大孔隙结构的β-TCP/Ti6Al4V复合支架。Pluronic F-127 和去离子水用作墨水制备的粘合剂和溶剂。本研究旨在研究复合生物陶瓷墨水的流变行为,并调查 DIW 工艺参数,如 Ti6Al4V 比例、填充比例和挤出压力。采用方框-贝肯响应面方法、方差分析、灵敏度和可取性方法对适合 DIW 的参数进行了实验、统计和数值优化。多目标优化考虑了输出响应,如制造的脚手架与原始尺寸的尺寸误差和抗压强度。结果表明,最佳值为固体负载 55 %v/v(β-TCP 40 %v/v,Ti6Al4V 15 %v/v)和 Pluronic 凝胶 45 %v/v,填充率 98 %,压力 6.36 巴。在优化条件下打印的支架的尺寸误差和抗压强度分别为 1.88 % 和 19 兆帕,具有适合骨再生的大孔和微孔,并通过 MTT 检测获得了令人满意的生物相容性。
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引用次数: 0
An automatic procedure for modelling patient-specific wrist orthosis 为特定患者腕部矫形器建模的自动程序
Q3 Medicine Pub Date : 2024-08-01 DOI: 10.1016/j.stlm.2024.100166
Michaela Servi, Roberto Magherini, Yary Volpe, Rocco Furferi

Background

In recent years, the treatment of wrist fractures has been the focus of numerous studies, particularly in the development of casts modeled on the patient's anatomy using additive manufacturing techniques. A 3D printed cast offers several advantages over traditional treatment methods, including washability, lightness, and ventilation.

Objective

This work introduces an automatic procedure for designing patient-specific wrist orthoses from a 3D scan of the arm using open-source mesh-processing libraries.

Methods

The procedure consists of seven steps that generate a customized orthosis model. Due to the absence of a single library capable of completing the entire modeling process, we defined the best execution strategy for each step and established a communication flow between the various blocks.

Results

The resulting orthosis comprises two halves, secured by three appropriately positioned bands and perforated with ventilation holes. The modeling procedure takes approximately 5 min to complete and was evaluated on 20 scans of arms of different shapes and sizes. The process proved to be fast, reliable, and suitable for direct use by medical personnel.

Conclusions

The developed automatic procedure for designing patient-specific wrist orthoses is efficient and effective, facilitating the use of 3D printed casts in medical practice.

背景近年来,腕部骨折的治疗一直是众多研究的重点,尤其是利用增材制造技术根据患者的解剖结构开发石膏模型。与传统治疗方法相比,3D 打印石膏具有可清洗性、轻便性和透气性等优点。 Objective This work introduces an automatic procedure for designing patient-specific wrist orthoses from a 3D scan of the arm using open-source mesh-processing libraries.Methods该程序包括七个步骤,可生成定制的矫形模型。由于缺乏能够完成整个建模过程的单一库,我们为每个步骤定义了最佳执行策略,并建立了各个模块之间的通信流程。结果最终生成的矫形器由两半组成,由三条位置适当的带子固定,并有通气孔。建模过程大约需要 5 分钟完成,并在 20 个不同形状和大小的手臂扫描中进行了评估。结论所开发的自动设计患者特定腕部矫形器的程序既高效又有效,有助于在医疗实践中使用三维打印模型。
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引用次数: 0
Geometric validation of a pediatric upper airways model made using a mainstream desktop 3D printer 使用主流桌面 3D 打印机制作的小儿上气道模型的几何验证
Q3 Medicine Pub Date : 2024-07-10 DOI: 10.1016/j.stlm.2024.100165
Pierre Cnockaert , Gregory Reychler , Renaud Menten , Jan Steckel , William Poncin

Background

Three-dimensional (3D) printing has become increasingly affordable. Several research projects used 3D printing to create in vitro upper airways model. However, studies using a mainstream desktop 3D printer never performed geometric validation of their model. The aim of this study was to perform geometric validation of a pediatric upper airways model printed with a mainstream desktop 3D printer.

Methods

Head computerized tomography (CT) scan of a 10-month-old female underwent segmentation between airways and surrounding anatomical structures. Airways segmentation allowed their measurement for further comparison with printed model. Head segmentation enabled the creation of a 3D printable volume file. To proceed to the geometric validation of the head model, the latter underwent a CT scan. Similar segmentation work was performed on the printed model for comparison. Overlap proportion between the original infant volume and the printed model as well as an average Hausdorff distance were calculated after manual alignment between the original and printed model.

Results

Volumes were 12.31 cm3 and 12.32 cm3 for the pediatric and the printed model upper airways, respectively (0.08% difference). Dice coefficient of original and printed model was 0.92. The average Hausdorff distance was 0.21 mm.

Conclusion

Desktop mainstream 3D printers can generate pediatric upper airway model with a high dimensional accuracy, as evidenced by our comprehensive geometrical validation.

背景三维(3D)打印变得越来越经济实惠。一些研究项目使用三维打印技术制作体外上呼吸道模型。然而,使用主流桌面 3D 打印机的研究从未对其模型进行几何验证。本研究的目的是对使用主流桌面 3D 打印机打印的小儿上呼吸道模型进行几何验证。方法对一名 10 个月大女性的头部计算机断层扫描(CT)结果进行气道和周围解剖结构的分割。气道分割后可对其进行测量,以便与打印模型进行进一步比较。对头部进行分割后,就可以创建可打印的三维体积文件。为了对头部模型进行几何验证,后者接受了 CT 扫描。在打印模型上也进行了类似的分割工作,以便进行比较。在对原始模型和打印模型进行手动对齐后,计算了原始婴儿体积和打印模型之间的重叠比例以及平均豪斯多夫距离。结果小儿和打印模型上气道的体积分别为 12.31 立方厘米和 12.32 立方厘米(相差 0.08%)。原始模型和印刷模型的骰子系数为 0.92。结论桌面主流三维打印机可以生成具有高尺寸精度的儿科上气道模型,我们的综合几何验证也证明了这一点。
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引用次数: 0
3D surgical planning method for lower jaw osteotomies applied to facial feminization surgery 应用于面部女性化手术的下颌骨截骨三维手术规划方法
Q3 Medicine Pub Date : 2024-07-08 DOI: 10.1016/j.stlm.2024.100164
Valeria Marin-Montealegre , Amelia R. Cardinali , Valentina Ríos Borras , M. Camila Ceballos-Santa , Jhon Jairo Osorio-Orozco , Iris V. Rivero

Our proposed method uses a three-dimensional (3D) measurement approach that focuses mainly on the lower jaw from basal, lateral, and frontal views applied to the volumetric skull model derived from a computed tomography (CT) of the head. Likewise, we discuss the geometrical features and clinical considerations involved in the 3D biomodeling of the surgical osteotomy. The workflow that allowed this virtual planning to be developed was composed of medical imaging processing software, data extraction software from images, and statistical software that allows the creation and generation of curve-fitting (nonlinear regression) graphs from data. Thirty-two (32) anatomical points were positioned, sixteen (16) measurements were taken, and two-dimensional (2D) sketches in three views (frontal, lateral, and inferior) were generated to overlap in a 3D environment, which informed the cutting of the desired bone segments. Implementing a nonlinear regression curve-fitting on the contours of the original jaws allowed optimal planning of the osteotomy. Desired cutting shapes were extrapolated for the front view by third-order equations, while for the side and bottom views, log-normal distribution curves and second-order polynomial curves were used, respectively. The reduction in the mandibular volume was between 6.55 and 10.27 %, with two of the most important measurements related to vertical reduction in the lateral views and the difference to determine gonion reduction.

我们提出的方法采用三维(3D)测量方法,主要侧重于下颌骨的基底、侧面和正面视图,并应用于头部计算机断层扫描(CT)得出的头骨体积模型。同样,我们还讨论了手术截骨的三维生物建模所涉及的几何特征和临床考虑因素。开发虚拟规划的工作流程由医学影像处理软件、图像数据提取软件和统计软件组成,其中统计软件可根据数据创建和生成曲线拟合(非线性回归)图。对 32 个解剖点进行了定位,进行了 16 次测量,生成了三个视图(正面、侧面和下部)的二维草图,并在三维环境中进行了重叠,为切割所需的骨段提供了依据。对原始颌骨轮廓进行非线性回归曲线拟合,可优化截骨规划。正视图的理想切割形状是通过三阶方程推算出来的,而侧视图和底视图则分别使用了对数正态分布曲线和二阶多项式曲线。下颌骨体积缩小了 6.55% 到 10.27%,其中两个最重要的测量值与侧视图中的垂直缩小和确定性腺缩小的差值有关。
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引用次数: 0
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Annals of 3D printed medicine
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