Pub Date : 2023-10-10DOI: 10.53043/2347-3894.acam11010
Xiaoteng Huang, Wenting Shan, Fei Ai, Xin Wei, Xia Liu
Shenfu injection (SFI) is widely used for treating endotoxin shock in China. In the present study, to investigate the anti-inflammatory effects of SFI and further explored the potential mechanism of HMGB1 nuclear translocation, we established a vitro cell model provoked by lipopolysaccharide (LPS), observed nucleocytoplasmic translocation of high mobility group box 1 (HMGB1) and the relationship between histone deacetylase 3 (HDAC3) and HMGB1 under SFI intervention. The results showed that SFI upregulated the transcription and expression of HDAC3 in RAW264.7 cells, inhibited the nuclear to cytoplasmic translocation of HMGB1 and its subsequent extracellular release, and depressed the secretion of HMGB1, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). However, targeted knockdown of HDAC3 induced an increase in HMGB1 translocation to the cytoplasm, and HMGB1 localization was not altered significantly following LPS stimulation. SFI failed to reverse the abnormal localization of HMGB1. These results suggested that SFI may inhibit LPS-induced HMGB1 nuclear translocation in RAW264.7 cells through upregulating HDAC3 expression, thereby inhibiting its downstream pathway and suppressing inflammatory response.
{"title":"SFI Reduces the Nucleocytoplasmic Transportation of HMGB1 by Upregulating HDAC3 in LPS-induced RAW264.7 Cells","authors":"Xiaoteng Huang, Wenting Shan, Fei Ai, Xin Wei, Xia Liu","doi":"10.53043/2347-3894.acam11010","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11010","url":null,"abstract":"Shenfu injection (SFI) is widely used for treating endotoxin shock in China. In the present study, to investigate the anti-inflammatory effects of SFI and further explored the potential mechanism of HMGB1 nuclear translocation, we established a vitro cell model provoked by lipopolysaccharide (LPS), observed nucleocytoplasmic translocation of high mobility group box 1 (HMGB1) and the relationship between histone deacetylase 3 (HDAC3) and HMGB1 under SFI intervention. The results showed that SFI upregulated the transcription and expression of HDAC3 in RAW264.7 cells, inhibited the nuclear to cytoplasmic translocation of HMGB1 and its subsequent extracellular release, and depressed the secretion of HMGB1, interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). However, targeted knockdown of HDAC3 induced an increase in HMGB1 translocation to the cytoplasm, and HMGB1 localization was not altered significantly following LPS stimulation. SFI failed to reverse the abnormal localization of HMGB1. These results suggested that SFI may inhibit LPS-induced HMGB1 nuclear translocation in RAW264.7 cells through upregulating HDAC3 expression, thereby inhibiting its downstream pathway and suppressing inflammatory response.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":"37 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136295185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.53043/2347-3894.acam11008
Mengqiu Li, Ziyang Lin, Minyi Li, Yongjin Zhu, Ting-ting Duan, Lanqing Meng, Xianlong Zhang, T. Xia, G. Lin, Xiuqing Lu, Zhenghai Li, Junzheng Yang
Sucralose is the most used artificial sweetener around the world, accounted for 30% of the global low-calorie sweetener market in 2016. According to the inspection of 19915 pre-packaged foods in Hong Kong, sucralose is the most used non-nutritive sweetener, followed by acesulfame. In addition to the food industry, sucralose can also be used as one kind of subsidiary materials for correcting taste in suspension agent, oral liquid, tablets, and granules, which could cover up the bitter taste of medicines such as aspirin or traditional Chinese medicine compound. The Food and Drug Administration (FDA) had approved the use of sucralose, but whether sucralose intake has effect on blood glucose is not clarified clearly. For this purpose, we collected and summarized the clinical studies to evaluate the effect of sucralose intake on blood glucose since 1996 from PubMed database, hope it may provide some evidence for sucralose applications in food excipient or drug sweet excipient.
{"title":"The Controversy: Whether Sucralose Could Increase Blood Glucose in The Body?","authors":"Mengqiu Li, Ziyang Lin, Minyi Li, Yongjin Zhu, Ting-ting Duan, Lanqing Meng, Xianlong Zhang, T. Xia, G. Lin, Xiuqing Lu, Zhenghai Li, Junzheng Yang","doi":"10.53043/2347-3894.acam11008","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11008","url":null,"abstract":"Sucralose is the most used artificial sweetener around the world, accounted for 30% of the global low-calorie sweetener market in 2016. According to the inspection of 19915 pre-packaged foods in Hong Kong, sucralose is the most used non-nutritive sweetener, followed by acesulfame. In addition to the food industry, sucralose can also be used as one kind of subsidiary materials for correcting taste in suspension agent, oral liquid, tablets, and granules, which could cover up the bitter taste of medicines such as aspirin or traditional Chinese medicine compound. The Food and Drug Administration (FDA) had approved the use of sucralose, but whether sucralose intake has effect on blood glucose is not clarified clearly. For this purpose, we collected and summarized the clinical studies to evaluate the effect of sucralose intake on blood glucose since 1996 from PubMed database, hope it may provide some evidence for sucralose applications in food excipient or drug sweet excipient.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48974386","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-24DOI: 10.53043/2347-3894.acam11009
Uwe Gröber
Magnesium is primarily found within the cell where it acts as a counter ion for the energy-rich ATP and nuclear acids. Magnesium is a cofactor in more than 600 pacemaker enzyme systems, encompassing approximately 80% of all known metabolic functions, that regulate elementary biochemical reactions in the body, including protein synthesis, muscle and nerve transmission, neuromuscular conduction, blood glucose control, and blood pressure regulation. Some magnesium-dependent enzymes are Na+/ K+-ATPase, hexokinase, creatine kinase, protein kinase, and cyclases. Magnesium is also necessary for structural function of proteins, nucleic acids, or mitochondria. It is required for DNA and RNA synthesis, reproduction, and for both aerobic and anaerobic energy production—oxidative phosphorylation and glycolysis either indirectly as a part of magnesium-ATP complex, or directly as an enzyme activator [1-3].
{"title":"Magnesium – The Metabolic Blockbuster","authors":"Uwe Gröber","doi":"10.53043/2347-3894.acam11009","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11009","url":null,"abstract":"Magnesium is primarily found within the cell where it acts as a counter ion for the energy-rich ATP and nuclear acids. Magnesium is a cofactor in more than 600 pacemaker enzyme systems, encompassing approximately 80% of all known metabolic functions, that regulate elementary biochemical reactions in the body, including protein synthesis, muscle and nerve transmission, neuromuscular conduction, blood glucose control, and blood pressure regulation. Some magnesium-dependent enzymes are Na+/ K+-ATPase, hexokinase, creatine kinase, protein kinase, and cyclases. Magnesium is also necessary for structural function of proteins, nucleic acids, or mitochondria. It is required for DNA and RNA synthesis, reproduction, and for both aerobic and anaerobic energy production—oxidative phosphorylation and glycolysis either indirectly as a part of magnesium-ATP complex, or directly as an enzyme activator [1-3].","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44311149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-05DOI: 10.53043/2347-3894.acam11004
T. Tennakoon, L.P.S. Vinodani, W. Warnasooriya, N. R. Amarasinghe, J. Madushani
Objectives: Honey has abundant medicinal properties. It is formed by floral nectar, swaps of plants, and the secretions of bees. The current study determined the physical, chemical, and biological characteristics of Sri Lankan raw bee honey varieties. Methods: A honey stock/library was created using local honey produced by Apis cerana, and Apis dorsata. Colour, conductivity, cell constant and pH were determined under physical tests while free acidity, water content and hydroxymethylfurfural content were determined under chemical tests. Evaluation of biological activities includes checking for antimicrobial activity, antioxidant activity and enzymatic activity. Results: The pH values recorded for A. dorsata and A. cerana honey ranged from 4.14–5.55 and 4.54–6.33, respectively. The electrical conductivity of all the samples ranged from 0.39-2.59 mS cm-1. The free acidity of honey samples of A. dorsata was between 25-81.7 meq/kg and that for A. cerana was between 40- 82.7 meq/kg. The hydroxymethylfurfural content of honey samples of A. dorsata and A. cerana were 9.38-49.50 mg/kg and 7.49-53.94 mg/kg, respectively. The two honey varieties were similar in chemical and physical characteristics tested except for water content where the water content of honey from A. dorsata was significantly higher than that of A. cerana. All honey samples investigated had antibacterial activity against gram-negative bacteria E. coli and gram-positive bacteria S. aureus. A. cerana honey had higher antioxidant activity than A. dorsata honey while A. dorsata honey had higher enzymatic activity than A. cerana honey. Conclusions: The present study reveals that the physiochemical properties of two honey varieties were similar except for water content while biological properties were significantly different. This study indicates that honey from both A. dorsata and A. cerana of Sri Lanka has potential therapeutic activities that can be used to develop natural drug formulations.
{"title":"Physical, Chemical and Biological Characteristics of Sri Lankan Bee Honey Varieties","authors":"T. Tennakoon, L.P.S. Vinodani, W. Warnasooriya, N. R. Amarasinghe, J. Madushani","doi":"10.53043/2347-3894.acam11004","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11004","url":null,"abstract":"Objectives: Honey has abundant medicinal properties. It is formed by floral nectar, swaps of plants, and the secretions of bees. The current study determined the physical, chemical, and biological characteristics of Sri Lankan raw bee honey varieties. Methods: A honey stock/library was created using local honey produced by Apis cerana, and Apis dorsata. Colour, conductivity, cell constant and pH were determined under physical tests while free acidity, water content and hydroxymethylfurfural content were determined under chemical tests. Evaluation of biological activities includes checking for antimicrobial activity, antioxidant activity and enzymatic activity. Results: The pH values recorded for A. dorsata and A. cerana honey ranged from 4.14–5.55 and 4.54–6.33, respectively. The electrical conductivity of all the samples ranged from 0.39-2.59 mS cm-1. The free acidity of honey samples of A. dorsata was between 25-81.7 meq/kg and that for A. cerana was between 40- 82.7 meq/kg. The hydroxymethylfurfural content of honey samples of A. dorsata and A. cerana were 9.38-49.50 mg/kg and 7.49-53.94 mg/kg, respectively. The two honey varieties were similar in chemical and physical characteristics tested except for water content where the water content of honey from A. dorsata was significantly higher than that of A. cerana. All honey samples investigated had antibacterial activity against gram-negative bacteria E. coli and gram-positive bacteria S. aureus. A. cerana honey had higher antioxidant activity than A. dorsata honey while A. dorsata honey had higher enzymatic activity than A. cerana honey. Conclusions: The present study reveals that the physiochemical properties of two honey varieties were similar except for water content while biological properties were significantly different. This study indicates that honey from both A. dorsata and A. cerana of Sri Lanka has potential therapeutic activities that can be used to develop natural drug formulations.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48663357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-05DOI: 10.53043/2347-3894.acam11006
Meijie Liang, W. Hong, Jiping Shao
1Objectives: To analyze the characteristics of gp120 gene sequence and protein structure of HIV-1 CRF01_AE and to predict B- and T-cell epitopes and to forecast binding sites between gp120 and mD1.22 by using bioinformatics in order to lay a foundation for the research and development of AIDS vaccines and peptide-based drugs. Methods: The gene sequences and the amino acid sequences of gp120 were obtained from NCBI GeneBank database. Physicochemical property, membrane-panning region, PTMs sites of gp120 and secondary structure were analyzed by Expasy ProtParam, TMpred, NetPhos and GlycoMine and SOPMA, respectively. Epitopes of B-cell were predicted by VaxiJen v2.0, AllerTOP v2.0 and IEDB while epitopes of T-cell were predicted by IEDB. Protein-protein docking were forecast by Z-dock, pymol and so on. Results: The calculation formula of gp120 is C1033H1633N299O319S9. The number of amino acids is 211, and the molecular weight is 23633.68. The theoretical isoelectric point (pI) is 7.76. It is estimated that 17 residues are phosphorylation sites, while gp120 has more than 5 glycosylation sites. The protein contains alpha helix (blue mark), beta turn (green mark), random coil (purple mark) and thin slice (red mark), which are 26.54%, 6.16%, 40.28% and 27.01% respectively. We selected the top 10 possible epitopes of B cells, but only the top 5 epitopes of MHC-I. The most possible binding site between gp120 and mD1.22 was showed. Conclusion: With the potential humoral immunity and cellular immunity, gp120 of HIV-1 is a promising target for HIV-1 treating. The interaction of gp120 with CD4 is the first step of HIV cycle which allows the entry of HIV into CD4+ T-cells. This study has analyzed and predicted the binding sites between gp120 and mD1.22 and the character and B-cell and T-cell epitopes of gp120, could be a foundation for subsequent study of developing vaccine and polypeptide drugs.
{"title":"Bioinformatics-based Prediction of Character of Envelope Glycoprotein and Analysis of Epitopes of B- and T-cell of gp120","authors":"Meijie Liang, W. Hong, Jiping Shao","doi":"10.53043/2347-3894.acam11006","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11006","url":null,"abstract":"1Objectives: To analyze the characteristics of gp120 gene sequence and protein structure of HIV-1 CRF01_AE and to predict B- and T-cell epitopes and to forecast binding sites between gp120 and mD1.22 by using bioinformatics in order to lay a foundation for the research and development of AIDS vaccines and peptide-based drugs. Methods: The gene sequences and the amino acid sequences of gp120 were obtained from NCBI GeneBank database. Physicochemical property, membrane-panning region, PTMs sites of gp120 and secondary structure were analyzed by Expasy ProtParam, TMpred, NetPhos and GlycoMine and SOPMA, respectively. Epitopes of B-cell were predicted by VaxiJen v2.0, AllerTOP v2.0 and IEDB while epitopes of T-cell were predicted by IEDB. Protein-protein docking were forecast by Z-dock, pymol and so on. Results: The calculation formula of gp120 is C1033H1633N299O319S9. The number of amino acids is 211, and the molecular weight is 23633.68. The theoretical isoelectric point (pI) is 7.76. It is estimated that 17 residues are phosphorylation sites, while gp120 has more than 5 glycosylation sites. The protein contains alpha helix (blue mark), beta turn (green mark), random coil (purple mark) and thin slice (red mark), which are 26.54%, 6.16%, 40.28% and 27.01% respectively. We selected the top 10 possible epitopes of B cells, but only the top 5 epitopes of MHC-I. The most possible binding site between gp120 and mD1.22 was showed. Conclusion: With the potential humoral immunity and cellular immunity, gp120 of HIV-1 is a promising target for HIV-1 treating. The interaction of gp120 with CD4 is the first step of HIV cycle which allows the entry of HIV into CD4+ T-cells. This study has analyzed and predicted the binding sites between gp120 and mD1.22 and the character and B-cell and T-cell epitopes of gp120, could be a foundation for subsequent study of developing vaccine and polypeptide drugs.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42221448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As the popularity of early screening of tumors, more and more people have detected pulmonary nodules, of which about 3.48% was lung cancer. However, false positive rates accounted for 1.4-23.3% according to trials reported. Therefore, pulmonary nodules may be over treated. In the clinical practice, we found that traditional Chinese medicine treatment makes some patients’ lung nodules shrink or even disappear but this effect needs to be approved. Therefore, we design this global network randomized controlled study (RCT) study to explore the efficacy and safety of traditional Chinese medicine in the treatment of pulmonary nodules. This study is a multicenter, open, prospective, randomized, controlled study. In this study, participants will be randomly assigned to traditional Chinese medicine or routine treatment observation group. About 166 patients with pulmonary nodules will be registered within 12 months and followed up for at least 2 years. The important feature of this clinical trial is to provide services to patients with pulmonary nodules worldwide by combining online and offline diagnosis and treatment, excluding interference factors such as region, climate, environment, eating habits etc. At the same time, it also will save costs and improves patient compliance. This trial design will also conduct subgroup analysis on the population of traditional Chinese medicine in the treatment of pulmonary nodules and establish a mathematical model to analyze the dominant population of traditional Chinese medicine in pulmonary nodules and the predictive factors of curative effect.
{"title":"Efficacy and Safety of Chinese Medicine in the Treatment of Patients with Pulmonary Nodules-A Multicenter Open Label Randomized Controlled Study: A Study Protocol for a Randomized Controlled Trial","authors":"Lixing Liu, Pei-xin Li, Qinglin Zhang, Rui Zhou, Lilian Feng","doi":"10.53043/2347-3894.acam11007","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11007","url":null,"abstract":"As the popularity of early screening of tumors, more and more people have detected pulmonary nodules, of which about 3.48% was lung cancer. However, false positive rates accounted for 1.4-23.3% according to trials reported. Therefore, pulmonary nodules may be over treated. In the clinical practice, we found that traditional Chinese medicine treatment makes some patients’ lung nodules shrink or even disappear but this effect needs to be approved. Therefore, we design this global network randomized controlled study (RCT) study to explore the efficacy and safety of traditional Chinese medicine in the treatment of pulmonary nodules. This study is a multicenter, open, prospective, randomized, controlled study. In this study, participants will be randomly assigned to traditional Chinese medicine or routine treatment observation group. About 166 patients with pulmonary nodules will be registered within 12 months and followed up for at least 2 years. The important feature of this clinical trial is to provide services to patients with pulmonary nodules worldwide by combining online and offline diagnosis and treatment, excluding interference factors such as region, climate, environment, eating habits etc. At the same time, it also will save costs and improves patient compliance. This trial design will also conduct subgroup analysis on the population of traditional Chinese medicine in the treatment of pulmonary nodules and establish a mathematical model to analyze the dominant population of traditional Chinese medicine in pulmonary nodules and the predictive factors of curative effect.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42489994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-03-05DOI: 10.53043/2347-3894.acam11005
Du Fusheng, Du Yanping, Ping Jiejie
{"title":"Rare Clinical Conditions Caused by Dry Eye","authors":"Du Fusheng, Du Yanping, Ping Jiejie","doi":"10.53043/2347-3894.acam11005","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11005","url":null,"abstract":"","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-03-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41995395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.53043/2347-3894.acam11002
Marian x Marian Mulligan*
{"title":"Forensic Naturopathy in the Treatment of Self Harm, Suicide Attempts, and Violence","authors":"Marian x Marian Mulligan*","doi":"10.53043/2347-3894.acam11002","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11002","url":null,"abstract":"","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41419695","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-02-10DOI: 10.53043/2347-3894.acam11001
T. Andoh, T. Shimizu
Clinical therapy of chronic inflammatory skin diseases such as atopic dermatitis is extremely difficult. Recently, the use of a number of traditional herbal medicines has been attempted to treat these skin diseases. Orengedokuto is a traditional herbal medicine consisting of four crude natural medicines, and in the field of dermatology, it is used to treat skin diseases accompanied by inflammation and pruritus. Our recent animal study showed that berberine, a major component of Orengedokuto, improves skin inflammation and itching in mice with atopy-like dermatitis. Furthermore, we identified EIF3F and MALT1 as factors involved in the suppression of cytokine production by berberine. In addition, berberine also activates AMP-activated protein kinase, which is involved in the expression of inflammatory and anti-inflammatory cytokines. In addition, berberine also attenuates epidermal hyperplasia though the inhibition of CDC6 expression. Taken together, these findings indicate that berberine improves inflammatory skin diseases by controlling gene expression related to both inflammation, anti-inflammation and keratinocyte hyperproliferation.
{"title":"The Molecular Mechanisms underlying the Therapeutic Effect of Berberine in Inflammatory Skin Diseases","authors":"T. Andoh, T. Shimizu","doi":"10.53043/2347-3894.acam11001","DOIUrl":"https://doi.org/10.53043/2347-3894.acam11001","url":null,"abstract":"Clinical therapy of chronic inflammatory skin diseases such as atopic dermatitis is extremely difficult. Recently, the use of a number of traditional herbal medicines has been attempted to treat these skin diseases. Orengedokuto is a traditional herbal medicine consisting of four crude natural medicines, and in the field of dermatology, it is used to treat skin diseases accompanied by inflammation and pruritus. Our recent animal study showed that berberine, a major component of Orengedokuto, improves skin inflammation and itching in mice with atopy-like dermatitis. Furthermore, we identified EIF3F and MALT1 as factors involved in the suppression of cytokine production by berberine. In addition, berberine also activates AMP-activated protein kinase, which is involved in the expression of inflammatory and anti-inflammatory cytokines. In addition, berberine also attenuates epidermal hyperplasia though the inhibition of CDC6 expression. Taken together, these findings indicate that berberine improves inflammatory skin diseases by controlling gene expression related to both inflammation, anti-inflammation and keratinocyte hyperproliferation.","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-02-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48582381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-01-01Epub Date: 2023-01-11DOI: 10.53043/2347-3894.acam11003
Kenneth Blum, Richard Green, Paul Mullen, David Han, Abdalla Bowirrat, Igor Elman, John B Floyd, Panayotis K Thanos, David Baron, Mark S Gold, Rajendra D Badgaiyan
{"title":"Reward Deficiency Syndrome Solution System (RDSSS) A Genetically Driven Putative Inducer of \"Dopamine Homeostasis\" as a Futuristic Alternative to Enhance Rehabilitation Instead of Incarceration.","authors":"Kenneth Blum, Richard Green, Paul Mullen, David Han, Abdalla Bowirrat, Igor Elman, John B Floyd, Panayotis K Thanos, David Baron, Mark S Gold, Rajendra D Badgaiyan","doi":"10.53043/2347-3894.acam11003","DOIUrl":"10.53043/2347-3894.acam11003","url":null,"abstract":"","PeriodicalId":72312,"journal":{"name":"Asian journal of complementary and alternative medicine : A-CAM","volume":"11 1","pages":"11-14"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9977164/pdf/nihms-1867722.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9434129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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