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Genetic Diversity of Ethiopian Durum Wheat (Triticum durum Desf.) Landrace Collections as Reveled by SSR Markers 埃塞俄比亚硬粒小麦的遗传多样性SSR标记揭示的地方品种收藏
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000413
M. Asmamaw, G. Keneni, K. Tesfaye
The genetic diversity of 141 Ethiopian durum wheat (Triticum durum Desf.) landrace collections and 19 improved varieties was analyzed using primers of 12 SSR markers in 2015. The experiment was conducted at the National Agricultural Biotechnology Laboratory, Holetta, Ethiopia. The difference between the longest and shortest amplified fragment size ranged from 100 to 350 bp. The highest variation in fragment size was observed for primer CFD 257 (250-350 bp) and the lowest was for primer CFA2278 (100-180 bp). All the 12 SSR primer pairs were polymorphic and generated a total of 74 alleles. The number of alleles per locus ranged from 4 for primers WMS 375, WMS 493, and CFD257 to 9 for primers WMS53, with a mean of 6 alleles per locus. Some loci were more monomorphic (WMS493, WMS516, and WMS532) with less gene flow than other loci such as WMS269 and WMS234. Based on the analysis of molecular variance (AMOVA), lower level (19%) of variation was observed among populations and higher level (81%) within population. The dendogram of cluster analysis based on neighbor-joining algorithm categorized the 160 durum wheat genotypes into three major clusters. Cluster I consisted 47, Cluster II 64, and Cluster III 49 genotypes. Based on the magnitude of the genetic distance (GD), more differentiations were observed between accessions in populations originated from different geographical regions in Ethiopia.
利用12个SSR标记的引物分析了2015年141个埃塞俄比亚硬粒小麦(Triticum durum Desf.)地方品种和19个改良品种的遗传多样性。该实验是在埃塞俄比亚Holetta的国家农业生物技术实验室进行的。最长和最短扩增片段大小的差异在100 ~ 350 bp之间。引物CFD 257片段大小变化最大(250-350 bp),引物CFA2278片段大小变化最小(100-180 bp)。12对SSR引物均具有多态性,共产生74个等位基因。引物wms375、wms493和CFD257每个位点的等位基因数为4个,引物WMS53每个位点的等位基因数为9个,平均每个位点6个。与WMS269和WMS234相比,WMS493、WMS516和WMS532具有较强的单态性,基因流较少。基于分子方差分析(AMOVA),种群间变异较低(19%),种群内变异较高(81%)。基于邻居加入算法的聚类分析树形图将160个硬粒小麦基因型划分为3大聚类。聚类1 47个,聚类2 64个,聚类3 49个。根据遗传距离(GD)的大小,在埃塞俄比亚不同地理区域的种群中观察到更多的分化。
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引用次数: 20
Plant water stress based precision irrigation using a wireless network of sensors and controllers 利用传感器和控制器的无线网络,基于植物水分胁迫的精确灌溉
Pub Date : 2018-12-12 DOI: 10.4172/2329-8863-c2-007
pShrini K Upadhyayap
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引用次数: 1
Evaluation of Tef ( Eragrostis tef (Zucc.) Trotter) Somaclones for Drought Tolerance Tef (Eragrostis Tef (Zucc.))的评价抗干旱体细胞克隆
Pub Date : 2018-08-01 DOI: 10.4172/2329-8863.1000385
Brikti Ferede, F. Mekbib, K. Assefa, S. Chanyalew, E. Abraha, Z. Tadele
Breeding for moisture stress tolerance by conventional methods is not satisfactory in view of current demand to increase crop productivity. Inducing somaclonal variation is a potential technique to obtain stress tolerant variants. The present study was conducted to evaluate the performance of tef somaclones (R1 generation) and their respective parental genotypes for drought tolerance. The materials used in this experiment were obtained from the R0 generation of previous in vitro culture experiment. Twelve seed derived somaclones; Melko 0, Melko 0.5, Melko 1, Melko 1.5, Gemechis 0, Gemechis 0.5, Gemechis 1, Gemechis 1.5, Pop12S20, Pop12S20.5, Pop12S21 and Pop12S21.5 and their respective parental genotypes (Melko, Gemechis and Pop12S2) were used under two set of experiments (i.e., under moisture stress and non-stress conditions) in pots under green house. The experiments were laid out in completely randomized design with three replications. Analysis of variance revealed that somaclones (R1 generation) and their parental genotypes showed highly significant (P ≤ 0.01) difference in all the measured traits under both moisture regimes. Comparisons were made between the somaclones and their parents under moisture stress and non-stress conditions for the measured traits. Under moisture stress condition superiority of the somaclones was observed except for number of spikelet/panicle and total number of tiller/plant. On the other hand, under non-stress condition better performance was observed from parental genotypes for number of spikelet/ panicle, panicle weight, panicle seed weight, plant weight, plant seed weight and grain yield. The results showed that all the somaclones and their parental genotypes were negatively affected by moisture stress for all studied parameters. However, somaclones were less affected by moisture stress as compared to the parental genotypes. Hence, in vitro culture induced somaclonal variation can effectively be used for selection and improvement of drought tolerant tef genotypes.
鉴于目前提高作物产量的需要,采用常规方法进行耐湿性育种已不能令人满意。诱导体细胞无性系变异是获得耐胁迫变异的一种潜在技术。本研究对tef体细胞无性系(R1代)及其亲本基因型的耐旱性进行了评价。本实验所用材料均为前人体外培养实验的R0代材料。12个种子衍生体细胞无性系;采用Melko 0、Melko 0.5、Melko 1、Melko 1.5、Gemechis 0、Gemechis 0.5、Gemechis 1、Gemechis 1.5、Pop12S20、Pop12S20.5、Pop12S21和Pop12S21.5及其亲本基因型(Melko、Gemechis和Pop12S2)在温室盆栽条件下进行水分胁迫和非胁迫两组试验。实验采用完全随机设计,重复3次。方差分析表明,在两种水分条件下,体细胞无性系(R1代)及其亲本基因型的所有性状差异均极显著(P≤0.01)。比较了水分胁迫和非水分胁迫条件下体细胞无性系及其亲本所测得性状的变化。在水分胁迫条件下,体细胞无性系除颖花/穗数和分蘖/株数外均表现出优势。另一方面,在非胁迫条件下,亲本基因型在穗粒数、穗重、穗粒重、株重、籽粒重和产量方面表现较好。结果表明,水分胁迫对所有体细胞无性系及其亲本基因型均有不利影响。与亲本基因型相比,体细胞无性系受水分胁迫的影响较小。因此,体外培养诱导的体细胞无性系变异可以有效地用于耐旱tef基因型的选择和改良。
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引用次数: 1
Genetic Variability Study of Yield and Yield Related Traits in Rice ( Oryza sativa L.) Genotypes 水稻产量及相关性状的遗传变异研究基因型
Pub Date : 2018-07-04 DOI: 10.4172/2329-8863.1000381
Beakal Tadesse Girma, Mihratu Amanuel Kitil, Desta Gebere Banje, Hailu Mengistu Biru, Tadiyos Bayisa Serbessa
Rice is one of the most important food crop which is a stable food for more than half of the world population providing one fifth of calorie consumption. In Ethiopia, it is considered as one of the target commodity that can promote agricultural production of the country. Ethiopia has a wide range of ecology that is suitable for rice production which is not suitable for other crops. Although the country has high potential, the lack well adaptable varieties is one of the major problem especially for irrigated areas. The present study was conducted with the objective of assessing the presence of genetic variability in 64 genotypes. The genotypes were evaluated from June to November 2016 at WARC experimental site using 8 × 8 simple lattice designs. The ANOVA revealed that there is highly significant difference between the genotypes in all studied traits. Also, GCV and PCV indicate the presence of variability in all studied traits. But PCV is a bit higher than GCV which indicated the influence of the environment in each trait although the effect was not much except in PL. The presence of high heritability and genetic advance in most of the traits indicates the presence of additive gene action. So, these traits can be improved through direct selection. The phenotypic correlation and principal component analysis showed that most of the traits evaluated are important for selection of high yielding genotypes and contributing their share for wider genetic variability of the genotypes. Therefore, the overall result indicates the presence of enough variability for development of improved rice varieties and the studied traits can be used for selection. But the experiment should be repeated by integrating more number of important traits in representative irrigated areas of the country.
大米是最重要的粮食作物之一,是世界上一半以上人口的稳定粮食,提供了五分之一的卡路里消耗。在埃塞俄比亚,它被认为是能够促进该国农业生产的目标商品之一。埃塞俄比亚有广泛的生态环境,适合种植水稻,而不适合种植其他作物。虽然这个国家有很大的潜力,但缺乏适应性强的品种是一个主要问题,特别是在灌溉区。本研究的目的是评估64个基因型中存在的遗传变异。2016年6 - 11月在WARC试验点采用8 × 8简单格设计进行基因型分析。方差分析显示,各基因型在各性状上均存在极显著差异。此外,GCV和PCV表明在所有研究性状中存在变异性。PCV略高于GCV,说明环境对各性状均有影响,但除PL外影响不大。大部分性状均表现出较高的遗传力和遗传先进性,说明存在加性基因作用。因此,这些性状可以通过直接选择得到改善。表型相关分析和主成分分析表明,所评价的大部分性状对高产基因型的选择具有重要意义,并对更广泛的基因型遗传变异性有贡献。因此,总体结果表明存在足够的变异以发展改良品种,所研究的性状可用于选择。但是应该在全国有代表性的灌溉区通过整合更多的重要性状来重复实验。
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引用次数: 30
The Causal Pathogen, Inoculum Sources and Alternative Hosts Studies of the Newly Emerged Gall Forming Faba Bean (Vicia faba) Disease in Ethiopia 埃塞俄比亚新发瘿状蚕豆病病原、接种源及替代寄主研究
Pub Date : 2018-06-04 DOI: 10.4172/2329-8863.1000368
G. Getaneh, Endalew Hailu, K. Sadessa, T. Alemu, Girma Megersa
Faba bean is the most important and dominant pulse crop of Ethiopia. In recent years the newly emerged disease were occurred and being threat to the production. The causal agent, disease alternative host and inoculums source were studied during the 2015-17 cropping season. As a result, based on field/plant symptom, morphometry and light microscopy, the pathogen were similar to previously identified faba bean gall disease which was caused by the fungal pathogen Olpidium viciae. It was observed that it can infect other hosts with mild severity. The alternate host study showed, the pathogen was largely soil and stubble born. Seeds from infected plant didn’t develop any disease symptom the consecutive three years.
蚕豆是埃塞俄比亚最重要和最主要的豆类作物。近年来新出现的病害不断出现,对生产构成威胁。研究了2015-17种植季的病原菌、病害替代寄主和菌源。结果表明,根据田间/植株症状、形态测定和光镜观察,该病原菌与先前鉴定的蚕豆瘿病相似,该病是由真菌病原菌Olpidium viciae引起的。据观察,它可以感染其他宿主,但严重程度较轻。交替寄主研究表明,病原菌主要来源于土壤和残茬。侵染植株的种子连续3年未出现任何病害症状。
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引用次数: 8
Estimation of Genetic Variability, Heritability and Genetic Advance for Essential Oil Yield and Related Traits in Genus Ocimum 精油产量及相关性状的遗传变异、遗传力及遗传进展
Pub Date : 2018-03-30 DOI: 10.4172/2329-8863.1000350
S. Smita, Kishori Rl
Assessing variability is fundamental to identify the most important traits in Ocimum improvement program. The objective of the present study was to estimate variability, heritability and genetic advance based on twelve morphological characters of Ocimum. The experiment was conducted in 2015/16 main cropping season at one location by using randomized complete block design with three replications. The results revealed highly significant differences (P<0.01) among genotypes for all characters considered. The phenotypic coefficient of variation (PCV) for all character was higher than genotypic coefficient of variation (GCV). The estimation of broad sense heritability ĥ2(BS)% was observed to be lower than those of broad sense heritability ĥ2(BS)% for all characters. Genetics advance was recorded as maximum for eugenol content (96.65). Highest heritability in broad sense ĥ2(BS)% recorded for days to maturity (99.46). On the basis of study accessions G-4, G-7, G-9, G-11, G-18 and G-25 were identified high oil of better quality. These accessions may be exploited for commercial cultivation.
评估可变性是识别Ocimum改进计划中最重要特征的基础。本研究的目的是基于12个形态特征来估计其变异度、遗传力和遗传进展。试验于2015/16主季在同一地点进行,采用随机完全区组设计,3个重复。结果表明,各性状基因型间差异极显著(P<0.01)。所有性状的表型变异系数(PCV)均高于基因型变异系数(GCV)。所有性状的广义遗传力ĥ2(BS)%估计值均低于广义遗传力ĥ2(BS)%估计值。丁香酚含量遗传进步最高(96.65)。最高广义遗传率ĥ2(BS)%记录的成熟期天数为99.46。在研究资料的基础上,确定了G-4、G-7、G-9、G-11、G-18和G-25为优质优质油。这些材料可用于商业种植。
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引用次数: 9
Evaluating the Potential Organic Manure for Improving Wheat Yield and Quality under Agro-Climatic Conditions of Pakistan 巴基斯坦农业气候条件下有机肥提高小麦产量和品质的潜力评价
Pub Date : 2018-03-28 DOI: 10.4172/2329-8863.1000349
A. Mukhtiar, A. Waqar, Khalil Mq, Muhammad Tariq, S. Muhammad, A. Hussain, A. Kamal
Use of organic manures for crop productivity not only improve crop production but also improving soil physicochemical properties. It also reduces soil and water pollution by acting as chelating agent for inorganic nutrients. Keeping these things in view an experiment was carried out aiming the use of different organic manures for improving crop productivity and to select a potential organic manure that improve crop productivity compared to others. All the manures were applied at the same rate 5 tons ha-1. The results revealed that all the wheat parameters were significantly affected by differences in nature of organic manures except days to emergence. Plots receiving Poultry manures has high Spikes m-2 (274), Grains spike-1 (60), more 1000 grain weight (42.287 g), high biological yield (11435 kg ha-1) and high grain yield (3996 kg ha-1). Similarly, Sheep manure had also great effect on wheat parameters such as higher emergence m-2(103), Tillers m-2 (308), plant height (104.50 cm), Grains spike-1 (60) and similar 1000 grain weight compared to poultry manures (42.200). Cattle and Farmyard manure were also found better compared to legume residue but were found less important in improving wheat quality compared to poultry and sheep manure. Similarly, legume residues showed very poor performance in improving wheat quality compared to other organic manures. Wheat parameters were found better performed when poultry or sheep manures applied at the rate of 5 tons ha-1. Therefore, it is recommended that application of sheep or poultry manures at the rate mentioned above improve wheat components under the agro-climatic conditions of Pakistan.
施用有机肥不仅能提高作物产量,还能改善土壤理化性质。它还可以作为无机养分的螯合剂,减少土壤和水的污染。考虑到这些问题,进行了一项实验,旨在使用不同的有机肥料来提高作物生产力,并选择一种潜在的有机肥料,与其他有机肥相比,提高作物生产力。所有肥料按相同的比率施用5吨每公顷。结果表明,除出苗期外,有机肥性质差异对小麦各项参数均有显著影响。鸡粪处理小区的穗数m-2高(274穗),穗数1高(60穗),千粒重高(42.287 g),生物产量高(11435 kg ha-1),籽粒产量高(3996 kg ha-1)。同样,羊粪对小麦的出苗率(m-2)(103)、分蘖率(m-2)(308)、株高(104.50 cm)、穗数(60)和千粒重(42.200)等参数也有很大影响。与豆类残留物相比,牛粪和农家肥的效果更好,但与家禽和羊粪相比,牛粪和农家肥在改善小麦品质方面的作用较小。与其他有机肥相比,豆科植物秸秆对小麦品质的改善效果也很差。以5吨/公顷施用家禽或绵羊粪便时,小麦参数表现较好。因此,建议在巴基斯坦的农业气候条件下,按上述比率施用羊粪或禽粪可改善小麦成分。
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引用次数: 5
Common Bean Improvement Status ( Phaseolus vulgaris L. ) in Ethiopia 菜豆改良现状(Phaseolus vulgaris L.)在埃塞俄比亚
Pub Date : 2018-03-26 DOI: 10.4172/2329-8863.1000347
Demelash Bb
Common bean (Phaseolus vulgaris L; 2n=22) is the most important food legume rich in protein, minerals, and vitamins where its protein content is cheap and easily affordable for the famers of the country. The crop plays great role in maintaining the fertility of the soil through fixing atmospheric nitrogen and thus keeping diversity and stability of the agricultural system. Due to its importance, the total area allocated for common bean crop production and the yield obtained in Ethiopia is 357,299.89 ha and 540,238.94 tons respectively. The productivity of white and red common bean is 1.41 ton/ha and 1.59 ton/ha respectively in 2016 planting season. Since 1970s common bean improvement has been started in Ethiopia and since then more than 50 common bean varieties for different traits and for different agro ecologies were released through conventional breeding in Ethiopia. Currently, in common bean breeding; gene pyramiding against common bean diseases like ALS (Angular Leaf Spot), CBB (Common Bean Blight) and anthracnose and diversity assessment for diseases like CBB and ALS has been started using MAS (Marker Assisted Selection) in Southern Agricultural Research Institute at Hawassa. Therefore the aim of this article is to review common bean improvement status and to indicate some improvement gaps in Ethiopia.
菜豆(Phaseolus vulgaris L;豆类是最重要的食物,富含蛋白质、矿物质和维生素,其蛋白质含量便宜,对该国的农民来说很容易负担得起。作物通过固定大气氮来维持土壤肥力,从而保持农业系统的多样性和稳定性。由于其重要性,埃塞俄比亚分配用于普通豆作物生产的总面积和产量分别为357,299.89公顷和540,238.94吨。2016年种植季白豆和红豆产量分别为1.41吨/公顷和1.59吨/公顷。自20世纪70年代以来,埃塞俄比亚开始进行普通豆改良,此后通过常规育种在埃塞俄比亚发布了50多个具有不同性状和不同农业生态的普通豆品种。目前,在普通豆类育种中;阿瓦萨南方农业研究所利用MAS (Marker Assisted Selection)技术开展了针对ALS (Angular Leaf Spot)、CBB (common bean Blight)和炭疽病等常见豆类疾病的基因金字塔构建和CBB和ALS等疾病的多样性评估。因此,本文的目的是回顾普通豆的改善状况,并指出一些改进差距在埃塞俄比亚。
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引用次数: 10
Productivity of Sweet Sorghum Genotypes under Contrasting Fertility Management for Food and Ethanol Production 甜高粱基因型在粮食和乙醇生产肥力管理对比下的生产力
Pub Date : 2018-03-26 DOI: 10.4172/2329-8863.1000348
E. Chalachew, M. Rebuma
Sweet sorghum (Sorghum bicolor (L.) Moench) has a good potential for ethanol production in Ethiopia. However, continual supply of feed stock to the distillery is one of the major constraints in sweet sorghum-based ethanol and grain yield productions. A study was carried out in Ethiopia during the 2015 to 2016 crop seasons to determine the performance of sweet sorghum genotypes for their stability or specific adaptability under contrasting fertilizer rates across environments. All agronomic characters were varied with the genotypes and environments. Grain yield, panicle weight and plant height were also significantly varied with fertilizations, but other agronomic characters were not varied with fertilizations. The ethanol yield and quality components varied with genotypes, environments and fertilizations, but brix % was not varied with fertilizations. The genotypes, E36-1, ICSR 93034 and IESV 92207 DL, produced greater estimated sugar, ethanol, juice and fresh stalk yield. The genotype, IESV 92207 DL, was the superior genotypes in grain yield and yield related traits, although it was second in brix % and ethanol yield followed by E36-1. At phenotypic and genotypic levels, grain yield was positive and significant correlations with panicle weight and panicle width. There was positive and significant (p<0.01) correlations between ethanol, juice, sugar and fresh stalk yields at both levels, this indicates the merits of these quality characters to improve ethanol yield.
甜高粱(sorghum bicolor, L.)Moench)在埃塞俄比亚有很好的乙醇生产潜力。然而,持续供应的原料,以酿酒厂是主要的制约因素之一,以甜高粱为基础的乙醇和粮食产量生产。2015年至2016年作物季期间,在埃塞俄比亚开展了一项研究,以确定甜高粱基因型在不同环境下不同施肥量下的稳定性或特定适应性。所有农艺性状随基因型和环境的不同而不同。籽粒产量、穗重和株高随施肥变化显著,但其他农艺性状不随施肥变化。乙醇产量和品质成分随基因型、环境和施肥的不同而不同,但甜度百分比不随施肥而变化。E36-1、ICSR 93034和IESV 92207 DL基因型的糖、乙醇、果汁和鲜茎产量估计更高。该基因型IESV 92207 DL在籽粒产量和产量相关性状上表现优异,但在糖度%和乙醇产量上表现次之,E36-1次之。在表型和基因型水平上,籽粒产量与穗重和穗宽呈显著正相关。在两个水平上,乙醇产量、果汁产量、糖产量和鲜茎产量均呈极显著正相关(p<0.01),说明这些品质性状在提高乙醇产量方面具有优势。
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引用次数: 3
Participatory Pre Extension Demonstration of Maize ( Zea Mays L.) Varieties with its Full Packages in South Ethiopia 玉米推广前参与式示范(Zea Mays L.)品种与它的完整包装在南埃塞俄比亚
Pub Date : 2018-03-02 DOI: 10.4172/2329-8863.1000342
S. Yokamo, Endrais Oyka
Participatory pre extension demonstration works of three variety (BH-546, BH-547 and BH-661) were conducted in 2016 at two FTC and 11 beneficiary farmers field by the main objective of enhancing the adoption and diffusion of the technology, to collect feedback information from the farmers and to study cost benefit analysis. All data were collected from 100 m2 demonstration plots and the collected data were analyzed through Genstat Software, Simple descriptive statistics and matrix rankings. Farmers were evaluate the demonstration plots three times i.e., at sowing, knee height and maturity stages. Average yield performances that were obtained from farmers field were 49 Quintal/ hectare, 53 Quintal/hectare and 43.5 Quintal/hectare of BH-546, BH-547 and BH-661 respectively and Yield that obtained from FTCs were 54 Quintal/hectare, 55 Quintal/hectare and 48 Quintal/hectare of BH-546, BH-547 and BH-661 respectively. BH-547 variety shows better yield performance than other variety. The net benefit that were obtained from BH-546, BH-547 and BH-661 were 23,750 ETB, 24,280 ETB and 20,400 ETB respectively. Finally, the demonstration sites were visited by organized farmer’s field day and farmers select those varieties by different criteria and rank at first BH-547 by its ability to tolerate drought, earliness, resistance to pest and disease and resistance to lodging. Secondly they select as best BH-546 by criteria of good seed emergency rate, earliness, resistance to disease and pest and ability to tolerate drought. Finally it is better to scale up both BH-547 and BH-546 in larger scale to enhance adoption and diffusion of variety.
2016年在2个农委会和11个受益农户开展了3个品种(BH-546、BH-547和BH-661)的参与式推广前示范工作,主要目的是加强技术的采用和推广,收集农户反馈信息,研究成本效益分析。所有数据采集自100m2的示范地块,通过Genstat软件、简单描述性统计和矩阵排序对采集数据进行分析。农民在播种、膝部高度和成熟期对示范地块进行了三次评价。BH-546、BH-547和BH-661在农民田间获得的平均产量分别为49、53和43.5 Quintal/公顷,BH-546、BH-547和BH-661在农民田间获得的平均产量分别为54、55和48 Quintal/公顷。品种BH-547表现出较好的产量表现。BH-546、BH-547和BH-661的净效益分别为23,750、24,280和20,400 ETB。最后,通过有组织的农民田间日访问示范点,农民根据不同的标准选择这些品种,并根据耐旱、早熟、抗病虫害和抗倒伏的能力将bh547排在第一位。其次,以种子抢救率高、早熟性好、抗病虫害能力强、抗旱能力强为标准,优选出最佳种子品种BH-546。最后,最好将BH-547和BH-546的规模都扩大,以促进品种的采用和扩散。
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引用次数: 1
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Advances in Crop Science and Technology
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