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Nitrogen Uptake and Use Efficiency of Irrigated Bread Wheat (Triticum aestivum L.) as Influenced by Seed and Nitrogen Fertilizer Rates at Werer, Afar National Regional State, Ethiopia 埃塞俄比亚阿法尔国家地区Werer地区种子和氮肥用量对灌溉面包小麦氮素吸收和利用效率的影响
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000418
Birke Bahiru, H. Ashagre, Mihratu Amanuel
An offseason field experiment was conducted in 2015-2016 at Werer Agricultural Research Center Middle Awash, Afar regional state to determine N-uptake and use efficiency of irrigated bread wheat (Triticum aestivum L.) as influenced by seed and nitrogen fertilizer rates. The experiment was laid down in a randomized complete block design (RCBD) with three replications. The treatments were factorial combination of five nitrogen levels (0, 23, 46, 69, and 92 kgNha-1) with four seed rates (75, 100, 125, and 150 kgha-1). The results of the study showed that both main and interaction effect of nitrogen and seed rates significantly affect N concentrations of the grain and straw, Nuptake, apparent nitrogen recovery, agronomic and physiological efficiencies, nitrogen use efficiency, grain protein content, and N harvest index. The apparent recovery, agronomic and physiological efficiencies and nitrogen use efficiencies decreased with increasing N-rate. Concentration of N and total nitrogen uptake in straw and grain, grain protein and NHI were increased as the N rate increased, while decreased with the increment of seed rate. In conclusion, rate of nitrogen fertilizer applied and seed rate affect the N-uptake and N-use efficiencies.
2015-2016年,在阿法尔州中部阿瓦什的Werer农业研究中心进行了淡季田间试验,研究了施氮量和施氮量对灌溉面包小麦(Triticum aestivum L.)氮素吸收和利用效率的影响。试验采用随机完全区组设计(RCBD), 3个重复。5个氮素水平(0、23、46、69和92 kgNha-1)和4个种子率(75、100、125和150 kgNha-1)的因子组合处理。结果表明,施氮量和种子量的主效应和互作效应对籽粒和秸秆氮素浓度、氮素吸收、表观氮素恢复、农艺和生理效率、氮素利用效率、籽粒蛋白质含量和氮素收获指数均有显著影响。表观恢复率、农艺生理效率和氮素利用效率随施氮量的增加而降低。秸秆和籽粒氮素浓度、总氮吸收量、籽粒蛋白质和氮肥含量均随施氮量的增加而增加,随播种率的增加而降低。综上所述,施氮量和种子量影响氮素吸收和氮素利用效率。
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引用次数: 0
Decade of South Ethiopian Coffee Improvement Activities at Awada Coffee Research Center 阿瓦达咖啡研究中心南埃塞俄比亚咖啡改良活动的十年
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000420
M. Gessese
Coffee improvement program of Ethiopia was aimed to develop widely adaptable and stable cultivars across all coffee growing regions of the country although there is a significant ecological variation that prevails between the major coffee growing regions. Assessing the feedback from users on the performance of released coffee cultivars, the national coffee research program realized the need to initiate coffee improvement programs for each coffee growing region that possessed specific coffee quality and fetch premium price in the world market. In effect, coffee improvement program was initiated for Awada Agricultural Research Center mandated to improve South Ethiopian coffee with the financial aid of the Government of Switzerland. To date about 580 arabica coffee accessions have been collected and maintained in the center in separate sets of collection and are under evaluation. Fourty-two (set I) and sixteen (set II) selections are under variety trials, twelve selections are in variety verification trial, five hybrids are under variety verification trial and four high yielding cultivars that possessed the typical quality of Yirgachefe or Sidama coffee types were released to coffee growers in the region. In this paper, coffee improvement activities, such as collection and evaluation of germplasm, variety development activities and genetic studies are reviewed.
埃塞俄比亚咖啡改良计划的目标是在该国所有咖啡种植区开发适应性广泛且稳定的品种,尽管在主要咖啡种植区之间存在显著的生态差异。国家咖啡研究计划评估了用户对发布的咖啡品种性能的反馈,意识到需要为每个咖啡种植区启动咖啡改进计划,这些咖啡种植区拥有特定的咖啡质量,并在世界市场上获得优质价格。实际上,咖啡改良计划是为阿瓦达农业研究中心发起的,该中心被授权在瑞士政府的财政援助下改善南埃塞俄比亚的咖啡。迄今为止,该中心已收集并保存了大约580件阿拉比卡咖啡制品,分装在不同的收藏品中,目前正在进行评估。42个品种(第一组)和16个品种(第二组)正在进行品种试验,12个品种正在进行品种验证试验,5个杂交品种正在进行品种验证试验,4个具有Yirgachefe或Sidama咖啡典型品质的高产品种已向该地区的咖啡种植者发布。本文综述了咖啡种质资源的收集与评价、品种开发和遗传研究等方面的改良活动。
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引用次数: 0
Characterization of Selected Maize Inbred Lines Adapted to Highland Agro-Ecologies of Ethiopia Using Morphological and Molecular Genetic Distances 利用形态和分子遗传距离对适应埃塞俄比亚高原农业生态的玉米自交系进行鉴定
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000421
Worknesh Terefe, A. Teklewold, K. Tesfaye
Characterization of available genetic diversity is a crucial step in effective crop improvement which provide basis for the analysis of combining ability and exploitation of heterosis of inbred lines in hybrid breeding. Characterization involves evaluation of quantitative and qualitative attributes of a given genotypes in order to differentiate their usefulness, structure, genetic variability and relationships among them. The objectives of this study were to characterize elite maize inbred lines adapted to highland agro-ecologies and classify groups of similar inbred lines by means of cluster and principal component analysis based on morpho-agronomic and SNP markers data. A total of twenty-three maize inbred lines of highland breeding department at Ambo Plant Protection Research Center formed the core plant materials in the current experiment for 23 morpho-agronomic traits and SNP markers characterization. The evaluated lines were sown in alpha lattice design 12 × 2. It was planted in two replications at each locations (Ambo and Holetta agricultural research centers). The inbred-lines were highly significantly different for all traits evaluated (p ≤ 0.01). The PCA indicated that the first nine principal components (PCs) with eigen value greater than unity accounted for 83% of the entire diversity among 23 inbred lines for all traits. Cluster analysis performed on the basis morpho-agronomic traits using unweighted paired group method arithmetic averages (UPGMA) grouped 23 tested lines in to five distinct classes and an outlier genotype whereas cluster analysis based on molecular resulted from distance matrix of genetic categorized the entries into four main groups. Five inbred lines (L5, L8, L18, L12 and L7) with comparatively high yielder and other phenotypic characters were selected using the morpho-agronomic traits and SNP based genotyping for cultivar development and germplasm utilization.
有效遗传多样性的鉴定是作物有效改良的关键步骤,为杂交育种中自交系配合力分析和杂种优势利用提供依据。表征包括对特定基因型的定量和定性属性进行评估,以便区分它们的有用性、结构、遗传变异性和它们之间的关系。本研究的目的是在形态农艺和SNP标记数据的基础上,采用聚类和主成分分析的方法对适应高原农业生态的玉米自交系进行鉴定,并对相似自交系进行类群分类。以安博植物保护研究中心高原育种部的23个玉米自交系作为本次试验的核心植物材料,对23个形态农艺性状和SNP标记进行了表征。评价线采用12 × 2 α格子设计播种。在每个地点(Ambo和Holetta农业研究中心)种植了两个重复。自交系各性状评价差异极显著(p≤0.01)。主成分分析表明,23个自交系中特征值大于1的前9个主成分占全部性状多样性的83%。基于形态农艺性状的聚类分析采用未加权配对组算法平均(UPGMA)将23个供试品系分为5个不同的类别和一个离群基因型,而基于遗传距离矩阵的分子结果聚类分析将供试品系分为4个主要类别。利用形态农艺性状和SNP基因分型技术,选择了产量较高的5个自交系(L5、L8、L18、L12和L7)进行品种开发和种质资源利用。
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引用次数: 2
Marker-Assisted Pyramiding Resistance Genes Against Angular Leaf Spot and Common Bacterial Blight Disease into Preferred Common Bean Cultivar "REDWOLAITA" 标记辅助锥体抗角斑病和普通白叶枯病基因在普通菜豆优选品种“红wolaita”中的应用
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000416
Y. Rezene, K. Tesfaye, C. Mukankusi, P. Gepts
Angular leaf spot (ALS) caused by Pseudocercospora griseola and common bacterial blight (CBB) caused by Xanthomonas campestris pv phaseoli X. campestris pv. phaseoli var. fuscans are the most economically important diseases of common bean production in Ethiopia. This research aims at pyramiding the Phg-2 R gene for angular leaf spot resistance and two CBB major resistance quantitative trait loci (RQTLs) into the background of the most popular and susceptible common bean cultivar “REDWOLAITA” (RW) with the aid of marker-assisted breeding method. Marker-assisted Parallel Back Crossing (MAPBC) breeding scheme with three separate parallel backcrossing streams were adopted for tracking three independent resistance loci linked to g796 (Phg-2 for ALS resistance) and, SU91 and SAP6 genetic markers from two different donor parents to the REDWOLAITA recurrent parent. The two donor parental lines VAX6 (with known RQTLs tagged by the SAP6 and SU91 genetic markers on linkage groups 10 and 8, respectively) and MEX54 with the Phg-2 R gene tagged by the g796 genetic marker at the linkage group 8 were used in the gene pyramiding program. After the BC4 generation, progenies that combined SAP6 and g796 genetic markers were created and selected from the BC4 inter-crossing of progenies. Then, further inter-crossing was made between selected progenies that combined the SAP6 and g796 genetic markers with selected progenies with the SU91 genetic marker. Finally, from this study we developed Monogenic Near Isogenic Lines (MNILs) with R genes tagged by the SAP6, g796, and SU91 molecular markers and polygenic PNILs with different gene combination includes MNILSAP6, MNILSU91 & MNILg796, polygenetic PNILs SAP6/g796, PNILs SU91/g796, PNILs SAP6/SU, PNILs SAP6/g796/SAP6, with more than 97% genome recovered from the RW genetic background. Marker-assisted backcrossing facilitated selection of progenies that combined good agronomic traits with resistance loci were constructed from the RW common bean cultivar genetic background and tested under the screening house condition. The developed lines showed high level of disease resistance to the strains of CBB and ALS present under the screening conditions. They were selected to be multiplied and tested under multiple environment, before varietal release and wider production. Developed MNILs with good agronomic background will also be used as alternative donor parent for the future gene pyramiding program.
由灰绿假cercospora griiseola引起的角斑病(ALS)和由campestris pv phaseoli X. campestris pv引起的普通细菌性枯萎病(CBB)。fuscans是埃塞俄比亚普通豆生产中最重要的经济病害。本研究旨在利用标记辅助育种的方法,将角斑病抗性phg - 2r基因和两个CBB主抗性数量性状位点(RQTLs)构建到最受欢迎和易感的普通豆品种“REDWOLAITA”(RW)的背景中。采用标记辅助平行回交(MAPBC)育种方案,对来自两个不同供体亲本的g796 (ALS抗性为Phg-2)、SU91和SAP6遗传标记的3个独立抗性位点进行了追踪。两个供体亲本VAX6(在连锁组10和连锁组8上分别有SAP6和SU91标记的已知RQTLs)和MEX54(在连锁组8上有g796标记的phg - 2r基因)被用于基因金字塔程序。在BC4代后,从后代的BC4杂交中选择SAP6和g796遗传标记组合的后代。将选择的带有SAP6和g796遗传标记的子代与选择的带有SU91遗传标记的子代进一步杂交。最后,我们开发了由SAP6、g796和SU91分子标记的R基因单基因近等基因系(MNILs)和不同基因组合的多基因PNILs,包括MNILSAP6、MNILSU91和MNILg796,多基因PNILs SAP6/g796, PNILs SU91/g796, PNILs SAP6/SU, PNILs SAP6/g796/SAP6, PNILs SAP6/g796/SAP6,从RW遗传背景中恢复了97%以上的基因组。利用RW普通豆品种的遗传背景,构建标记辅助回交,筛选农艺性状优良、抗病位点结合的后代,并在筛选室条件下进行试验。在筛选条件下,对CBB和ALS菌株均表现出较高的抗病性。在品种发布和广泛生产之前,选择它们在多种环境下进行繁殖和试验。具有良好农艺背景的已开发的mnil也将被用作未来基因金字塔计划的替代供体亲本。
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引用次数: 1
Effect of Industrial Crops Production in the Emerging Dynamic Needs of Natural Resources Utilization on Farmers' Perception and Adaptation of Agricultural Development Projects (In Case of Wolkait Sugar Development Project), North Western, Ethiopia 新兴的自然资源利用动态需求中的工业作物生产对农民对农业发展项目的认知和适应的影响(以Wolkait糖业发展项目为例),埃塞俄比亚西北部
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000417
Temesgen Kebede Dubale, Worku Mengesha Estifo
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引用次数: 0
Impact of Zinc and Organic Manure on Wheat Productivity and Soil Fertility 锌和有机肥对小麦生产力和土壤肥力的影响
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000414
Waqar Ali, Mukhtiar Ali, J. Iqbal, M. Khalil, Z. Ahmad
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引用次数: 7
Herbicide Interactions between Glufosinate and Three Fomesafen-Containing Herbicide Products as Affected by Weed Size and Spray Droplet Size 草铵膦与三种含氟草胺除草剂的相互作用受杂草大小和喷雾滴度的影响
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000415
C. J. Meyer, J. Norsworthy, G. Kruger
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引用次数: 0
Evaluation of Bread Wheat (Triticum aestivum L.) Genotypes for Yield Potential and Related Traits Under High Temperature Stress Condition at Middle Awash, Ethiopia 面包小麦(Triticum aestivum L.)的评价埃塞俄比亚中部阿瓦什地区高温胁迫条件下产量潜力及相关性状基因型研究
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000410
Tadiyos Bayisa, E. Habte, Mihratu Amanuel
Information on the extent and performance of genotypes present in a crop species under high temperature stress are essential for effective crop adaptation program. To generate such information 36 bread wheat genotypes with three released varieties (Fentalle, Amibara and Werer-2) were tested in Triple Lattice Design with three replication at Werer agricultural research center, Afar in 2017. The objective of the study were to develop best performed bread wheat genotypes that adapted to middle Awash area and to identify best performed bread wheat genotypes for yield under high temperature stress condition. The data generated from the experiment were subjected to analysis of variance. The analysis of variances of bread wheat genotypes evaluated for 15 traits revealed highly significant difference between the genotypes for most traits and significant difference among genotypes for days to emergence. It was interesting to note that genotypes identified for short grain filling period and early maturing were G-4, G-18, G-3, G-26 and G-15 with maturity days of 70, 76, 77, 77 and 79 days after sowing respectively, than the released check variety Werer-2 (84 days). Similarly genotypes G-16, G-22, G-28, G-32 and G-34 had better required low canopy temperature reading than released check varieties Fentalle, Amibara and Werer-2. The most important genotypes for showing excellent performance on grain yield per hectare obtained from G-2, G-7 and G-21 with grain yield of 2479 kh/ha, 3039 kg/ha and 2409 kg/ha respectively, than the best performed released check variety Werer-2 (2362 kilo gram per hectare). Hence, from the current results it has been observed adequate existence of traits showing adaptability in the studied genotypes and this could be exploited in future bread wheat selection and breeding for high temperature stress condition.
了解作物在高温胁迫下基因型变化的程度和表现,对有效的作物适应计划至关重要。为了获得这些信息,2017年在阿法尔的Werer农业研究中心对36个面包小麦基因型和3个已发布品种(Fentalle、Amibara和Werer-2)进行了三重格子设计和3个重复试验。本研究的目的是寻找适合中阿瓦什地区的最佳面包小麦基因型,以及在高温胁迫条件下最佳产量表现的面包小麦基因型。对实验产生的数据进行方差分析。对面包小麦15个性状的基因型方差分析显示,大部分性状的基因型间存在极显著差异,且在出苗期基因型间存在显著差异。值得注意的是,籽粒灌浆期短和早熟的基因型分别为G-4、G-18、G-3、G-26和G-15,其成熟期分别为播种后70、76、77、77和79 d,而对照品种维尔-2的成熟期为84 d。基因型G-16、G-22、G-28、G-32和G-34比对照品种Fentalle、Amibara和Werer-2更需要低冠温读数。G-2、G-7和G-21在单产方面表现优异,产量分别为2479、3039和2409公斤/公顷,优于表现最好的对照品种Werer-2(2362公斤/公顷)。因此,从目前的研究结果来看,所研究的基因型中存在足够的适应性性状,可以为今后面包小麦在高温胁迫条件下的选择和育种提供参考。
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引用次数: 5
Soil Test and Tissue Analysis Based Nitrogen and Phosphorus Fertilizer Applications on Teff [Eragrostis tef (Zucc.) Trotter] Cultivars in North Shewa Central Highlands, Ethiopia 基于土壤试验和组织分析的氮磷肥在Teff (Eragrostis tef (Zucc.))上的应用埃塞俄比亚谢瓦中部高地北部的栽培品种
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000411
Temesgen Kebede Dubale
Soil fertility studies and crop improvement have brought remarkable change in crop production particularly in teff in Ethiopia. Differences in soil status affect productivities of various cultivars in which their nutritional demand is different and increasing. This elucidates that fertilizer rate studies are dynamic and increasing time to time across crop cultivars. Fertilizer rate recommendations need to be specific soil and tissue test based and should be done repeatedly for any cultivar to maximize the inherent potential yield of the crop. Field experiment was laid out to test teff cultivars to a soil and tissue test based NP fertilizer applications. Soil samples were collected and subjected to a soil test in respective methods employed to determine the physicochemical property of a soil. High available P, medium organic carbon content moderately low total N content and pH of 7.57 were obtained from the experimental site. Plant tissue samples were collected from a 2 × 2 m2 plot and subjected to analysis to determine tissue nitrogen and phosphorus contents. N application significantly increased the concentrations of nitrogen and phosphorus in shoot tissue and grain yield. However, application of P at different rates did not significantly affected any of the parameters. This might be due to unavailability of P for the plant due to fixation and hence examination must continue to identify real causes.
土壤肥力研究和作物改良为作物生产带来了显著变化,特别是埃塞俄比亚的苔麸。土壤状况的差异影响着不同品种的生产力,不同品种的营养需求不同且不断增加。这说明施肥研究是动态的,并且在作物品种间是不断增加的。肥料用量的建议需要根据具体的土壤和组织试验,并应反复进行,以最大限度地提高作物的内在潜在产量。通过田间试验,对油菜品种进行了土壤和组织施肥试验。收集土壤样品,并采用各自的方法进行土壤试验,以确定土壤的物理化学性质。试验点速效磷含量高,有机碳含量中等,全氮含量中低,pH为7.57。在一个2 × 2 m2的地块上采集植物组织样品,分析测定组织氮、磷含量。施氮显著提高了地上部组织氮磷浓度和籽粒产量。不同施磷量对各参数均无显著影响。这可能是由于固定导致植物无法获得P,因此必须继续检查以确定真正的原因。
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引用次数: 2
Antifungal Activities of Some Essential Oils Against Fusarium oxysporum of Rosemary and Sage Plants 几种精油对迷迭香和鼠尾草尖孢镰刀菌的抑菌活性研究
Pub Date : 2019-01-01 DOI: 10.4172/2329-8863.1000419
Mihiret Mekonnen, Ano Wariyo, Guddisa Hilu
Rosemary (Rosmarinus officinalis L.) is a common household aromatic and medicinal plant belonging to the Labiatae family and sage (Salvia officinalis) Lamiaceae family is aromatic, rather woody perennial low shrub. This study aimed some antifungal effects of plant essential oils were investigated against fungal disease agent Fusarium oxysporum on mycilial growth inhibition in vitro and disease development reserve in vivo conditions. The tissue pieces were aseptically transferred to petridishes containing a potato dextrose agar medium supplemented with streptomycin sulfate at the rate of three to five pieces per plate. Fusarium oxysporum was grown for 7-10 days on PDA at 27°C. The concentrations were tested at 20, 40, 60 and 80 μl. The same quantity of PDA Fusarium oxysporum also established in control. F. oxysporum was cut with a sterile cork borer from the edge of actively growing cultures on PDA plates. At 20 μl, 40 μl, 60 μl and 80 μl concentration, lemon grass (100%) growth inhibition was confirmed against Fusarium wilt followed by spearmint (85.00%) and minimum inhibition recorded 25.00% at Eucalyptus. As conclusion lemon grass and spear mint essential oils demonstrated the maximum antagonistic effect against the Fusarium wilt, whereas Eucalyptus inhibit the list amount of growth pathogen.
迷迭香(Rosmarinus officinalis L.)是一种常见的家用芳香和药用植物,属于唇形科和鼠尾草(Salvia officinalis) Lamiaceae科是芳香的,相当木质的多年生低矮灌木。本研究旨在探讨植物精油对真菌病原菌尖孢镰刀菌的体外抑菌作用和体内疾病发展储备作用。将组织片无菌转移到含有添加硫酸链霉素的马铃薯葡萄糖琼脂培养基的培养皿中,每板3至5片。尖孢镰刀菌在27℃的PDA培养基上培养7 ~ 10 d。分别在20、40、60、80 μl浓度下检测。在对照中也发现了相同数量的镰刀菌。用无菌软木钻从PDA平板上活跃生长的培养物边缘切割尖孢菌。在浓度为20、40、60和80 μl时,柠檬草对枯萎病的抑制率为100%,其次是绿薄荷(85.00%),桉树对枯萎病的抑制率最低为25.00%。结果表明,柠檬草和薄荷精油对枯萎病的拮抗作用最大,桉树精油对枯萎病生长病原菌的拮抗作用最大。
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引用次数: 4
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Advances in Crop Science and Technology
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