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Magnetic Microrobots with Folate Targeting for Drug Delivery. 具有叶酸靶向药物递送的磁性微型机器人。
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.34133/cbsystems.0019
Min Ye, Yan Zhou, Hongyu Zhao, Xiaopu Wang
Untethered microrobots can be used for cargo delivery (e.g., drug molecules, stem cells, and genes) targeting designated areas. However, it is not enough to just reach the lesion site, as some drugs can only play the best therapeutic effect within the cells. To this end, folic acid (FA) was introduced into microrobots in this work as a key to mediate endocytosis of drugs into cells. The microrobots here were fabricated with biodegradable gelatin methacryloyl (GelMA) and modified with magnetic metal–organic framework (MOF). The porous structure of MOF and the hydrogel network of polymerized GelMA were used for the loading of enough FA and anticancer drug doxorubicin (DOX) respectively. Utilizing the magnetic property of magnetic MOF, these microrobots can gather around the lesion site with the navigation of magnetic fields. The combination effects of FA targeting and magnetic navigation substantially improve the anticancer efficiency of these microrobots. The result shows that the cancer cells inhibition rate of microrobots with FA can be up to 93%, while that of the ones without FA was only 78%. The introduction of FA is a useful method to improve the drug transportation ability of microrobots, providing a meaningful reference for further research.
不受束缚的微型机器人可用于针对指定区域的货物运输(例如,药物分子、干细胞和基因)。然而,仅仅到达病变部位是不够的,因为有些药物只能在细胞内发挥最佳的治疗效果。为此,本研究将叶酸(folic acid, FA)作为介导药物内吞作用的关键物质引入微机器人。该微机器人由可生物降解的明胶甲基丙烯酰(GelMA)和磁性金属有机框架(MOF)修饰而成。利用MOF的多孔结构和聚合GelMA的水凝胶网络分别装载足量FA和抗癌药物DOX。利用磁性MOF的磁性,这些微型机器人可以在磁场的导航下聚集在病变部位周围。FA靶向和磁导航的联合作用大大提高了这些微型机器人的抗癌效率。结果表明,添加FA的微型机器人对癌细胞的抑制率可达93%,而不添加FA的微型机器人的抑制率仅为78%。FA的引入为提高微型机器人的药物运输能力提供了有益的方法,为进一步的研究提供了有意义的参考。
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引用次数: 8
Carbon-Based Stimuli-Responsive Nanomaterials: Classification and Application. 碳基刺激响应纳米材料:分类与应用。
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2023-01-01 DOI: 10.34133/cbsystems.0022
Chen Zhao, Jun Kang, Yuwen Li, Yan Wang, Xiaoying Tang, Zhenqi Jiang

Carbon-based nanomaterials, including carbon nanotubes, carbon nanospheres, and carbon nanofibers, are becoming a research hotspot due to their unique structure and good mechanical, thermal, electrical, optical, and chemical properties. With the development of material synthesis technology, they can be functionalized and used in various fields such as energy, environment, and biomedicine. In particular, stimuli-responsive carbon-based nanomaterials have stood out in recent years because of their smart behavior. Researchers have applied carbon-based nanomaterials to different disease treatments based on their stimulus-response properties. In this paper, based on stimuli-responsive carbon-based nanomaterials' morphology, we categorize them into carbon nanotubes, carbon nanospheres, and carbon nanofibers according to their morphology. Then, their applications in probes, bioimaging, tumor therapy, and other fields are discussed. Finally, we address the advantages and disadvantages of carbon-based stimuli-responsive nanomaterials and discuss their future perspective.

碳基纳米材料,包括碳纳米管、碳纳米球和碳纳米纤维,由于其独特的结构和良好的力学、热学、电学、光学和化学性能而成为研究热点。随着材料合成技术的发展,它们可以被功能化并应用于能源、环境、生物医药等各个领域。特别是,刺激响应碳基纳米材料近年来因其智能行为而脱颖而出。研究人员根据碳基纳米材料的刺激反应特性,将其应用于不同的疾病治疗。本文基于碳基刺激响应纳米材料的形态特征,将其分为碳纳米管、碳纳米球和碳纳米纤维。然后讨论了它们在探针、生物成像、肿瘤治疗等领域的应用。最后,我们讨论了碳基刺激响应纳米材料的优点和缺点,并讨论了它们的未来前景。
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引用次数: 6
A Systematic Review of the Use of Commercial Wearable Activity Trackers for Monitoring Recovery in Individuals Undergoing Total Hip Replacement Surgery. 关于使用商用可穿戴活动追踪器监测全髋关节置换手术患者恢复情况的系统性综述》(A Systematic Review of Use of Commercial Wearable Activity Trackers for Monitoring Recovery in Individuals Undergoing Total Hip Replacement Surgery)。
IF 10.5 Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-10-26 eCollection Date: 2022-01-01 DOI: 10.34133/2022/9794641
Nasibeh Babaei, Negin Hannani, Nader Jafarnia Dabanloo, Shayan Bahadori

The innovation of wearable devices is advancing rapidly. Activity monitors can be used to improve the total hip replacement (THR) patients' recovery process and reduce costs. This systematic review assessed the body-worn accelerometers used in studies to enhance the rehabilitation process and monitor THR patients. Electronic databases such as Cochrane Database of Systematic Reviews library, CINAHL CompleteVR, Science Citation Index, and MedlineVR from January 2000 to January 2022 were searched. Due to inclusion criteria, fourteen eligible studies that utilised commercial wearable technology to monitor physical activity both before and after THR were identified. Their evidence quality was assessed with RoB 2.0 and ROBINS-I. This study demonstrates that wearable device technology might be feasible to predict, monitor, and detect physical activity following THR. They could be used as a motivational tool to increase patients' mobility and enhance the recovery process. Also, wearable activity monitors could provide a better insight into the individual's activity level in contrast to subjective self-reported questionnaires. However, they have some limitations, and further evidence is needed to establish this technology as the primary device in THR rehabilitation.

可穿戴设备的创新发展日新月异。活动监测器可用于改善全髋关节置换术(THR)患者的康复过程并降低成本。本系统性综述评估了研究中用于改善全髋关节置换术患者康复过程和监测全髋关节置换术患者的体戴式加速度计。研究人员检索了 2000 年 1 月至 2022 年 1 月期间的电子数据库,如 Cochrane Database of Systematic Reviews library、CINAHL CompleteVR、Science Citation Index 和 MedlineVR。根据纳入标准,确定了 14 项符合条件的研究,这些研究利用商业可穿戴技术来监测 THR 前后的体力活动。这些研究的证据质量通过 RoB 2.0 和 ROBINS-I 进行了评估。这项研究表明,可穿戴设备技术在预测、监测和检测全脊椎十字路口术后的体力活动方面是可行的。它们可以作为一种激励工具,增加患者的活动能力,促进康复进程。此外,与主观的自我报告问卷相比,可穿戴活动监测器能更好地了解个人的活动水平。不过,可穿戴活动监测器也有一些局限性,要将这种技术确定为 THR 康复的主要设备,还需要进一步的证据。
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引用次数: 0
Efficacy of Biological and Physical Enhancement on Targeted Muscle Reinnervation 生物和物理强化对靶向肌肉再支配的疗效
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-09-15 DOI: 10.34133/2022/9759265
Siyang Zhong, Zijun Zhang, Huan Su, Chenyang Li, Yifeng Lin, Wei Lu, Zhendong Jiang, Lin Yang
Targeted muscle reinnervation (TMR) is a microsurgical repair technique to reconstruct the anatomical structure between the distal nerve and the muscle stump to provide more myoelectric information to the artificially intelligent prosthesis. Postoperative functional electrical stimulation treatment of the patient's denervated muscle or proximal nerve stump as well as nerve growth factor injection is effective in promoting nerve regeneration and muscle function recovery. In this experiment, we successfully established a TMR rat model and divided Sprague-Dawley (SD) adult male rats into TMR group, TMR + FES group, and TMR + NGF group according to TMR and whether they received FES treatment or NGF injection after surgery, and the recovery effect of rat neuromuscular function was assessed by analyzing EMG signals. Through the experiments, we confirmed that growth factor supplementation and low-frequency electrical stimulation can effectively promote the regeneration of the transplanted nerve as well as significantly enhance the motor function of the target muscle and have a positive effect on the regeneration of the transplanted nerve.
靶向肌肉再支配(TMR)是一种显微外科修复技术,用于重建远端神经和肌肉残端之间的解剖结构,为人工智能假肢提供更多的肌电信息。术后对患者失神经肌肉或近端神经残端进行功能性电刺激治疗,并注射神经生长因子,可有效促进神经再生和肌肉功能恢复。在本实验中,我们成功地建立了TMR大鼠模型,并将Sprague-Dawley(SD)成年雄性大鼠分为TMR组、TMR + FES组和TMR + NGF组根据TMR,术后是否接受FES治疗或注射NGF,并通过分析EMG信号评估大鼠神经肌肉功能的恢复效果。通过实验证实,补充生长因子和低频电刺激能有效促进移植神经的再生,并能显著增强靶肌的运动功能,对移植神经的重建有积极作用。
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引用次数: 3
Platelet Detection Based on Improved YOLO_v3 基于改进YOLO_v3的血小板检测
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-09-14 DOI: 10.34133/2022/9780569
Renting Liu, Chunhui Ren, Miaomiao Fu, Z. Chu, Jiuchuan Guo
Platelet detection and counting play a greatly significant role in medical field, especially in routine blood tests which can be used to judge blood status and diagnose related diseases. Therefore, platelet detection is valuable for diagnosing related blood diseases such as liver-related diseases. Blood analyzers and visual microscope counting were widely used for platelet detection, but the experimental procedure took nearly 20 minutes and can only be performed by a professional doctor. In recent years, technological breakthroughs in artificial intelligence have made it possible to detect red blood cells through deep learning methods. However, due to the inaccessibility of platelet datasets and the small size of platelets, deep learning-based platelet detection studies are almost nonexistent. In this paper, we carried out experiments for platelet detection based on commonly used object detection models, such as Single Shot Multibox Detector (SSD), RetinaNet, Faster_rcnn, and You Only Look Once_v3 (YOLO_v3). Compared with the other three models, YOLO_v3 can detect platelets more effectively. And we proposed three ideas for improvement based on YOLO_v3. Our study demonstrated that YOLO_v3 can be adopted for platelet detection accurately and in real time. We also implemented YOLO_v3 with multiscale fusion, YOLO_v3 with anchor box clustering, and YOLO_v3 with match parameter on our self-created dataset and, respectively, achieved 1.8% higher average precision (AP), 2.38% higher AP, and 2.05% higher AP than YOLO_v3. The comprehensive experiments revealed that YOLO_v3 with the improved ideas performs better in platelet detection than YOLO_v3.
血小板的检测与计数在医学领域,特别是在血液常规检查中具有重要的作用,可用于判断血液状况和诊断相关疾病。因此,血小板检测对肝脏相关疾病等相关血液疾病的诊断具有重要价值。血液分析仪和视觉显微镜计数被广泛用于血小板检测,但实验过程耗时近20分钟,只能由专业医生完成。近年来,人工智能的技术突破使得通过深度学习方法检测红细胞成为可能。然而,由于血小板数据集的不可访问性和血小板体积小,基于深度学习的血小板检测研究几乎不存在。本文基于常用的目标检测模型(Single Shot Multibox Detector, SSD)、RetinaNet、Faster_rcnn、You Only Look Once_v3 (YOLO_v3)进行了血小板检测实验。与其他三种模型相比,YOLO_v3能更有效地检测血小板。并提出了基于YOLO_v3的三个改进思路。我们的研究表明,YOLO_v3可以准确、实时地用于血小板检测。在自建数据集上实现了基于多尺度融合的YOLO_v3、基于锚盒聚类的YOLO_v3和基于匹配参数的YOLO_v3,分别比YOLO_v3提高了1.8%、2.38%和2.05%的平均精度。综合实验表明,采用改进思路的YOLO_v3在血小板检测方面优于YOLO_v3。
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引用次数: 7
Graphdiyne-Related Materials in Biomedical Applications and Their Potential in Peripheral Nerve Tissue Engineering 石墨烯相关材料在生物医学中的应用及其在周围神经组织工程中的潜力
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-09-10 DOI: 10.34133/2022/9892526
Xiao Li, Huiquan Jiang, Ning He, Wei-En Yuan, Y. Qian, Y. Ouyang
Graphdiyne (GDY) is a new member of the family of carbon-based nanomaterials with hybridized carbon atoms of sp and sp2, including α, β, γ, and (6,6,12)-GDY, which differ in their percentage of acetylene bonds. The unique structure of GDY provides many attractive features, such as uniformly distributed pores, highly π-conjugated structure, high thermal stability, low toxicity, biodegradability, large specific surface area, tunable electrical conductivity, and remarkable thermal conductivity. Therefore, GDY is widely used in energy storage, catalysis, and energy fields, in addition to biomedical fields, such as biosensing, cancer therapy, drug delivery, radiation protection, and tissue engineering. In this review, we first discuss the synthesis of GDY with different shapes, including nanotubes, nanowires, nanowalls, and nanosheets. Second, we present the research progress in the biomedical field in recent years, along with the biodegradability and biocompatibility of GDY based on the existing literature. Subsequently, we present recent research results on the use of nanomaterials in peripheral nerve regeneration (PNR). Based on the wide application of nanomaterials in PNR and the remarkable properties of GDY, we predict the prospects and current challenges of GDY-based materials for PNR.
石墨烯(GDY)是碳基纳米材料家族中的一个新成员,具有sp和sp2的杂化碳原子,包括α、β、γ和(6,6,12)-GDY,它们在乙炔键中的百分比不同。GDY独特的结构提供了许多吸引人的特征,如均匀分布的孔隙、高度π-共轭的结构、高热稳定性、低毒性、生物降解性、大比表面积、可调的电导率和显著的热导率。因此,GDY广泛应用于储能、催化和能源领域,以及生物医学领域,如生物传感、癌症治疗、药物输送、辐射防护和组织工程。在这篇综述中,我们首先讨论了不同形状的GDY的合成,包括纳米管、纳米线、纳米壁和纳米片。其次,在现有文献的基础上,介绍了近年来生物医学领域的研究进展,以及GDY的生物降解性和生物相容性。随后,我们介绍了纳米材料在外周神经再生(PNR)中的最新研究结果。基于纳米材料在PNR中的广泛应用和GDY的显著性能,我们预测了GDY基材料用于PNR的前景和当前的挑战。
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引用次数: 8
The Inverse Problems for Computational Psychophysiology: Opinions and Insights 计算心理生理学的反问题:观点和见解
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-08-24 DOI: 10.34133/2022/9850248
B. Hu, Kun Qian, Ye Zhang, Jian Shen, B. Schuller
Since a long time, measuring the psychological status of subjects in a quantitative paradigm is a challenging problem in the scientific community. It is known that there is not a direct way to measure the psychological quantities [1], whereas an emerging methodology, i.e., computational psychophysiology (CPP), was introduced [2]. The core idea of CPP is to explore the link between the psychological quantities and the physiological quantities, which the latter ones can be measured via ubiquitous equipment (e.g., a braincomputer interface device). Psychiatric diseases are usually accompanied by abnormal psychological status, which can be objectively quantified by psychophysiological quantities. Evaluating psychiatric diseases is of great significance for mental health. With the fast development of artificial intelligence, big data, wearables, and the internet of things, we can observe successful achievements in finding quantitative methods for evaluating the degree of psychiatric diseases (e.g., depression) under the guidance of CPP. Nevertheless, the underlying mechanisms of these engineering milestones are still “up in the air” [3]. Investigating the fundamentals of CPP is a prerequisite for strengthening our power to extend the knowledge frontiers of mental health and benefit from clinical practice. D. R. Bach et al. proposed the concept of the “psychophysiological inverse problem,” claiming that psychologists use the peripheral physiological quantities to infer psychological quantities [4]. In particular, compared to other domains (e.g., intelligent disease diagnosis), understanding the mechanism of the mind could even benefit the development of novel clinical treatment methods for psychiatric disease. Therefore, the inverse problem tool cannot only facilitate a more personalised and precised medicine but also help discover the inherited characteristics of the psychophysiology. It is reasonable to think that the fundamental mechanism of CPP can be validated and/or interpreted by introducing the methodology of mathematical inverse problems. By the language of mathematical inverse problems [5], the computational psychophysiological problems can be formulated through an abstract equation,
长期以来,用定量范式测量被试的心理状态一直是科学界面临的难题。众所周知,没有一种直接的方法来测量心理量[1],而一种新兴的方法,即计算心理生理学(CPP),被引入[1]。CPP的核心思想是探索心理量和生理量之间的联系,后者可以通过无处不在的设备(如脑机接口设备)来测量。精神疾病通常伴有异常的心理状态,可以用心理生理量客观地量化。精神疾病的评估对心理健康具有重要意义。随着人工智能、大数据、可穿戴设备和物联网的快速发展,在CPP的指导下,我们可以看到精神疾病(如抑郁症)程度的定量评估方法的成功成果。然而,这些工程里程碑的潜在机制仍然“悬而未决”。研究CPP的基本原理是加强我们扩展精神卫生知识前沿和从临床实践中获益的先决条件。d.r.b ach等人提出了“心理生理逆问题”的概念,声称心理学家使用外围生理量来推断心理量[4]。特别是,与其他领域(例如,智能疾病诊断)相比,了解心理机制甚至可能有利于精神疾病的新型临床治疗方法的发展。因此,逆问题工具不仅可以促进更个性化和精确的医学,而且有助于发现心理生理的遗传特征。有理由认为,CPP的基本机制可以通过引入数学逆问题的方法来验证和/或解释。用数学逆问题[5]的语言来说,计算心理生理问题可以用一个抽象的方程来表述,
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引用次数: 2
A Survey on Design, Actuation, Modeling, and Control of Continuum Robot. 连续体机器人的设计、驱动、建模与控制综述
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-07-25 eCollection Date: 2022-01-01 DOI: 10.34133/2022/9754697
Jingyu Zhang, Qin Fang, Pingyu Xiang, Danying Sun, Yanan Xue, Rui Jin, Ke Qiu, Rong Xiong, Yue Wang, Haojian Lu

In this paper, we describe the advances in the design, actuation, modeling, and control field of continuum robots. After decades of pioneering research, many innovative structural design and actuation methods have arisen. Untethered magnetic robots are a good example; its external actuation characteristic allows for miniaturization, and they have gotten a lot of interest from academics. Furthermore, continuum robots with proprioceptive abilities are also studied. In modeling, modeling approaches based on continuum mechanics and geometric shaping hypothesis have made significant progress after years of research. Geometric exact continuum mechanics yields apparent computing efficiency via discrete modeling when combined with numerical analytic methods such that many effective model-based control methods have been realized. In the control, closed-loop and hybrid control methods offer great accuracy and resilience of motion control when combined with sensor feedback information. On the other hand, the advancement of machine learning has made modeling and control of continuum robots easier. The data-driven modeling technique simplifies modeling and improves anti-interference and generalization abilities. This paper discusses the current development and challenges of continuum robots in the above fields and provides prospects for the future.

本文介绍了连续体机器人的设计、驱动、建模和控制等方面的研究进展。经过几十年的开创性研究,出现了许多创新的结构设计和驱动方法。无系绳磁力机器人就是一个很好的例子;它的外部驱动特性允许小型化,他们已经得到了很多学者的兴趣。此外,还研究了具有本体感觉能力的连续体机器人。在建模方面,基于连续介质力学和几何成形假设的建模方法经过多年的研究取得了重大进展。几何精确连续介质力学通过离散建模与数值分析方法相结合具有明显的计算效率,从而实现了许多有效的基于模型的控制方法。在控制中,闭环和混合控制方法结合传感器反馈信息提供了很高的运动控制精度和弹性。另一方面,机器学习的进步使连续体机器人的建模和控制变得更加容易。数据驱动建模技术简化了建模,提高了抗干扰能力和泛化能力。本文讨论了连续统机器人在上述领域的发展现状和面临的挑战,并对未来进行了展望。
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引用次数: 0
Experimental Verification on Steering Flight of Honeybee by Electrical Stimulation 电刺激控制蜜蜂飞行的实验验证
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-07-21 DOI: 10.34133/2022/9895837
Li Yu, Jieliang Zhao, Zhiyun Ma, Wenzhong Wang, Shaoze Yan, Yue Jin, Yu Fang
The artificial locomotion control strategy is the fundamental technique to ensure the accomplishment of the preset assignments for cyborg insects. The existing research has recognized that the electrical stimulation applied to the optic lobes was an appropriate flight control strategy for small insects represented by honeybee. This control technique has been confirmed to be effective for honeybee flight initiation and cessation. However, its regulation effect on steering locomotion has not been fully verified. Here, we investigated the steering control effect of honeybee by applying electrical stimulation signals with different duty cycles and frequencies on the unilateral optic lobes and screened the stimulus parameters with the highest response successful rate. Moreover, we confirmed the effectiveness of steering control by verifying the presence of rotation torque on tethered honeybees and the body orientation change of crawling honeybees. Our study will contribute some reliable parameter references to the motion control of cyborg honeybees.
人工运动控制策略是保证机器人昆虫完成预设任务的基本技术。现有研究已经认识到,对以蜜蜂为代表的小昆虫来说,对视叶进行电刺激是一种合适的飞行控制策略。这种控制技术已被证实对蜜蜂飞行的开始和停止是有效的。然而,其对转向运动的调节作用尚未得到充分验证。在这里,我们通过对单侧视叶施加不同占空比和频率的电刺激信号来研究蜜蜂的转向控制效果,并筛选出响应成功率最高的刺激参数。此外,我们通过验证系留蜜蜂的旋转扭矩和爬行蜜蜂的身体方向变化,证实了转向控制的有效性。我们的研究将为半机械人蜜蜂的运动控制提供一些可靠的参数参考。
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引用次数: 3
Recognition of Drivers’ Hard and Soft Braking Intentions Based on Hybrid Brain-Computer Interfaces 基于脑机混合接口的驾驶员硬、软制动意图识别
Q1 ENGINEERING, BIOMEDICAL Pub Date : 2022-07-20 DOI: 10.34133/2022/9847652
Jiawei Ju, Aberham Genetu Feleke, Longxi Luo, Xinan Fan
In this paper, we propose simultaneous and sequential hybrid brain-computer interfaces (hBCIs) that incorporate electroencephalography (EEG) and electromyography (EMG) signals to classify drivers’ hard braking, soft braking, and normal driving intentions to better assist driving for the first time. The simultaneous hBCIs adopt a feature-level fusion strategy (hBCI-FL) and classifier-level fusion strategies (hBCIs-CL). The sequential hBCIs include the hBCI-SE1, where EEG signals are prioritized to detect hard braking, and hBCI-SE2, where EMG signals are prioritized to detect hard braking. Experimental results show that the proposed hBCI-SE1 with spectral features and the one-vs-rest classification strategy performs best with an average system accuracy of 96.37% among hBCIs. This work is valuable for developing human-centric intelligent assistant driving systems to improve driving safety and driving comfort and promote the application of BCIs.
在本文中,我们提出了同步和顺序混合脑机接口(hBCIs),结合脑电图(EEG)和肌电图(EMG)信号来分类驾驶员的硬制动,软制动和正常驾驶意图,以更好地辅助驾驶。同时hbci采用特征级融合策略(hBCI-FL)和分类器级融合策略(hbci - cl)。顺序hbci包括hBCI-SE1,其中脑电图信号优先检测硬制动,以及hBCI-SE2,其中肌电信号优先检测硬制动。实验结果表明,结合光谱特征和1 -vs-rest分类策略的hBCI-SE1在hbci中表现最好,平均系统准确率为96.37%。该工作对于开发以人为中心的智能辅助驾驶系统,提高驾驶安全性和舒适性,促进脑机接口的应用具有重要价值。
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引用次数: 12
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Cyborg and bionic systems (Washington, D.C.)
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