Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02307.X
M. H. Solaas
Presence of Australia-SH antigen in serum has been studied by agar gel double diffusion, electron microscopy and complement fixation tests. Serum from 30 patients suffering from serum hepatitis or infectious hepatitis, and serum from 10 apparently healthy blood donors have been included in the investigation. In serum from four of the patients with Australia-SH antigen positive serum hepatitis, the presence of the antigen has been studied during the course of the disease. The agar gel double diffusion test was less sensitive than the other two methods, although the sensitivity of this method was raised by increasing the amount of the serum sample to be examined. The present electron microscopical test seems to be at least as sensitive as the complement fixation test. However, the electron microscopical test may be less specific than previously believed.
{"title":"Detection of Australia-SH antigen in serum. A comparison of the electron microscopical, agar gel double diffusion, and complement fixation tests.","authors":"M. H. Solaas","doi":"10.1111/J.1699-0463.1974.TB02307.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02307.X","url":null,"abstract":"Presence of Australia-SH antigen in serum has been studied by agar gel double diffusion, electron microscopy and complement fixation tests. Serum from 30 patients suffering from serum hepatitis or infectious hepatitis, and serum from 10 apparently healthy blood donors have been included in the investigation. In serum from four of the patients with Australia-SH antigen positive serum hepatitis, the presence of the antigen has been studied during the course of the disease. The agar gel double diffusion test was less sensitive than the other two methods, although the sensitivity of this method was raised by increasing the amount of the serum sample to be examined. The present electron microscopical test seems to be at least as sensitive as the complement fixation test. However, the electron microscopical test may be less specific than previously believed.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"31 1","pages":"151-63"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74495975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04323.X
H. Thörne, H. Hallander
A provisional phage set suggested by Davidson for typing of “bovine” Staphylococcus aureus was compared to the international phage set in the typing of 225 strains isolated from bovine mastitis in 61 herds in central Sweden. At RTD 44.9 per cent of the strains were typable with the provisional phage set and 30.7 per cent with the international set. Corresponding figures at 1000 × RTD were 74.7 and 69.8 per cent. With both sets a total typability of 79.1 per cent was achieved. The following phage set proved most useful: 29, 52 A, 84, 3 A, 6, 53, 75, 77, 42 D, 107, 1363/14, 78, S 1, S 6, 883, 54, 71, 81.
{"title":"Phage typing of Staphylococcus aureus from bovine mastitis. A comparison of phages according to Davidson to the conventional phage set.","authors":"H. Thörne, H. Hallander","doi":"10.1111/J.1699-0463.1970.TB04323.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04323.X","url":null,"abstract":"A provisional phage set suggested by Davidson for typing of “bovine” Staphylococcus aureus was compared to the international phage set in the typing of 225 strains isolated from bovine mastitis in 61 herds in central Sweden. At RTD 44.9 per cent of the strains were typable with the provisional phage set and 30.7 per cent with the international set. Corresponding figures at 1000 × RTD were 74.7 and 69.8 per cent. With both sets a total typability of 79.1 per cent was achieved. The following phage set proved most useful: 29, 52 A, 84, 3 A, 6, 53, 75, 77, 42 D, 107, 1363/14, 78, S 1, S 6, 883, 54, 71, 81.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"78 4 1","pages":"425-8"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81467227","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/j.1699-0463.1971.tb00037.x
M. Degré
The effect of infection with parainfluenza 1 virus or Haemophilus influenzae b on the mucosa of respiratory tract of mice was studied. A section of trachea just above the bifurcation was examined histologically for the presence of destructive changes and leukocyte accumulation in the epithelium and lamina propria. Polymorphonuclear (PMN) leukocyte accumulation was a prominent sign after both viral and bacterial inoculation. Viral inoculation produced more frequent and more pronounced inflammatory changes than bacterial inoculation. Disruption of the basement membrane was observed only following virus inoculation. Gross evidence of pneumonia was seen more frequently among virus inoculated animals. The histological changes were progressive with time of survival up to the third day after inoculation. The bacterial titer in homogenates of lung tissue correlated with the number of lobes affected with pneumonia and to some extent with the accumulation of PMN leukocytes in the tracheal epithelium. Bacteria, normally present in the upper respiratory tract, were isolated from a high proportion of virus inoculated lungs. The findings are compatible with the concept that parainfluenza 1 virus infection facilitates a secondary bacterial invasion because of functional impairment or destruction of the epithelium.
{"title":"Synergistic effect in viral-bacterial infection. 4. The leukocyte response to parainfluenza virus infection in the respiratory tract of mice, and the influence of antibiotics on the development of infection.","authors":"M. Degré","doi":"10.1111/j.1699-0463.1971.tb00037.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1971.tb00037.x","url":null,"abstract":"The effect of infection with parainfluenza 1 virus or Haemophilus influenzae b on the mucosa of respiratory tract of mice was studied. A section of trachea just above the bifurcation was examined histologically for the presence of destructive changes and leukocyte accumulation in the epithelium and lamina propria. Polymorphonuclear (PMN) leukocyte accumulation was a prominent sign after both viral and bacterial inoculation. Viral inoculation produced more frequent and more pronounced inflammatory changes than bacterial inoculation. Disruption of the basement membrane was observed only following virus inoculation. Gross evidence of pneumonia was seen more frequently among virus inoculated animals. The histological changes were progressive with time of survival up to the third day after inoculation. The bacterial titer in homogenates of lung tissue correlated with the number of lobes affected with pneumonia and to some extent with the accumulation of PMN leukocytes in the tracheal epithelium. Bacteria, normally present in the upper respiratory tract, were isolated from a high proportion of virus inoculated lungs. The findings are compatible with the concept that parainfluenza 1 virus infection facilitates a secondary bacterial invasion because of functional impairment or destruction of the epithelium.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"18 1","pages":"88-94"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85878084","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04283.X
O. S. Mikkelsen
An account is given of the distribution of 958 strains of Ps. aeruginosa among 14 O groups, investigated by means of a tube agglutination technique and heat-treated antigen suspensions. Use has been made of unabsorbed O sera corresponding to Habs 12 O groups, an O group 13 from Sandvik's material, and an O group 14 prepared with a strain from the present material. 75 per cent of the strains, which originated chiefly from patients in a hospital environment, could be O group determined. 55 per cent of the strains belonged to O groups 6, 2, 5, 3 and 11. Strains belonging to O groups 2, 5 and 6 were the ones most frequently occurring in a hospital environment. The results showed that strains belonging to O groups 2 and 5 were more frequent than O group 6 strains in a hospital environment and that they colonized more easily than O group 6 strains wounds, urinary tract, ears, pleura and lower respiratory tract, whereas strains belonging to O groups 2 and 5 and O group 6 were equally frequent in the upper respiratory tract and in the large intestine (stools). The frequent occurrence of strains belonging to O groups 2 and 5 in samples from blood and central nervous system can be explained by the finding of these O group strains at sites from where Ps. aeruginosa is presumed to advance into the circulation and central nervous system.
{"title":"Serotyping of Pseudomonas aeruginosa. 2. Results of an O group classification.","authors":"O. S. Mikkelsen","doi":"10.1111/J.1699-0463.1970.TB04283.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04283.X","url":null,"abstract":"An account is given of the distribution of 958 strains of Ps. aeruginosa among 14 O groups, investigated by means of a tube agglutination technique and heat-treated antigen suspensions. Use has been made of unabsorbed O sera corresponding to Habs 12 O groups, an O group 13 from Sandvik's material, and an O group 14 prepared with a strain from the present material. 75 per cent of the strains, which originated chiefly from patients in a hospital environment, could be O group determined. 55 per cent of the strains belonged to O groups 6, 2, 5, 3 and 11. Strains belonging to O groups 2, 5 and 6 were the ones most frequently occurring in a hospital environment. The results showed that strains belonging to O groups 2 and 5 were more frequent than O group 6 strains in a hospital environment and that they colonized more easily than O group 6 strains wounds, urinary tract, ears, pleura and lower respiratory tract, whereas strains belonging to O groups 2 and 5 and O group 6 were equally frequent in the upper respiratory tract and in the large intestine (stools). The frequent occurrence of strains belonging to O groups 2 and 5 in samples from blood and central nervous system can be explained by the finding of these O group strains at sites from where Ps. aeruginosa is presumed to advance into the circulation and central nervous system.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"215 1","pages":"163-75"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77555761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02361.X
J. Ursing
The biochemical reactions of 8 isolates of Proteus inconstans biotype A, 83 isolates of P. inconstans biotype B and 18 isolates of P. rettgeri were compared with 14 isolates that had the same biochemical pattern as biotype B of P. inconstans but for a positive urease reaction. On the basis of comparison it is shown that the atypical isolates must be considered as urease positive variants of P. inconstans biotype B. The urease reaction is accordingly not an absolutely reliable feature in differentiating P. inconstans from the other Proteus species. This confirms the observations of some previous authors but contradicts the more generally accepted view. Attention is drawn to the possible value of a negative galactose fermentation test in characterizing strains of P. inconstans biotype A.
{"title":"BIOCHEMICAL STUDY OF PROTEUS INCONSTANS (PROVIDENCIA).","authors":"J. Ursing","doi":"10.1111/J.1699-0463.1974.TB02361.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02361.X","url":null,"abstract":"The biochemical reactions of 8 isolates of Proteus inconstans biotype A, 83 isolates of P. inconstans biotype B and 18 isolates of P. rettgeri were compared with 14 isolates that had the same biochemical pattern as biotype B of P. inconstans but for a positive urease reaction. On the basis of comparison it is shown that the atypical isolates must be considered as urease positive variants of P. inconstans biotype B. The urease reaction is accordingly not an absolutely reliable feature in differentiating P. inconstans from the other Proteus species. This confirms the observations of some previous authors but contradicts the more generally accepted view. Attention is drawn to the possible value of a negative galactose fermentation test in characterizing strains of P. inconstans biotype A.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"31 1","pages":"527-532"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86928309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04286.X
E. Bindseil
{"title":"Immunity to Ascaris suum. 4. The effect of different stimulations upon challenge with Ascaris suum in mice.","authors":"E. Bindseil","doi":"10.1111/J.1699-0463.1970.TB04286.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04286.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"51 1","pages":"191-5"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75144677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04312.X
S. Jyssum, K. Jyssum
Meningococcal extracts mediate an incorporation of 32P in the presence of Mg44 and ADP which is improved by the addition of EDTA. A major part of this incorporation is due to a polynucleotide phosphorylase activity, and is independent of electron flux. But the endogenous respiration is also accompanied by a phosphorylation. When the cytochrome oxidase is inhibited by KCN phosphorylation occurs concomitantly with the endogenous reduction of added mammalian ferricytochrome c. This phosphorylation is nearly doubled when KHCO3 is included in the system. It has not been possible to demonstrate an oxidative phosphorylation on the electron-oxygen transport level in meningococcal extracts with NADH, NADPH or ferrocytochrome c as electron sources. The phosphorylation connected with electron flux, and its enhancement by KHCO3 have been discussed in relation to the CO2 requirements typical for N. meningitidis.
{"title":"Endogenous incorporation of 32 P in Neisseria meningitidis. I. The effects of CO2 and electron flux.","authors":"S. Jyssum, K. Jyssum","doi":"10.1111/J.1699-0463.1970.TB04312.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04312.X","url":null,"abstract":"Meningococcal extracts mediate an incorporation of 32P in the presence of Mg44 and ADP which is improved by the addition of EDTA. A major part of this incorporation is due to a polynucleotide phosphorylase activity, and is independent of electron flux. But the endogenous respiration is also accompanied by a phosphorylation. When the cytochrome oxidase is inhibited by KCN phosphorylation occurs concomitantly with the endogenous reduction of added mammalian ferricytochrome c. This phosphorylation is nearly doubled when KHCO3 is included in the system. It has not been possible to demonstrate an oxidative phosphorylation on the electron-oxygen transport level in meningococcal extracts with NADH, NADPH or ferrocytochrome c as electron sources. The phosphorylation connected with electron flux, and its enhancement by KHCO3 have been discussed in relation to the CO2 requirements typical for N. meningitidis.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"61 1","pages":"337-42"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86628559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/j.1699-0463.1974.tb02298.x
Thomsen Ac, S. Rosendal
{"title":"Mycoplasmosis: experimental pyelonephritis in rats. The effect of secondary infection with Escherichia coli.","authors":"Thomsen Ac, S. Rosendal","doi":"10.1111/j.1699-0463.1974.tb02298.x","DOIUrl":"https://doi.org/10.1111/j.1699-0463.1974.tb02298.x","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"1 1","pages":"94-98"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88468166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04319.X
A. Wallerström
The “Epidermophyton factor” (EPF) was formed best on cultivation in medium where the reaction was maintained alkaline. The substance gave the chemical colour reactions expected of a steroid. In these reactions and in comparative chromatography EPF closely resembled fucidin, but it was found to be a less acid substance. Nor was EPF identical with 3-keto-fusidic acid, helvolic acid or cephalosporin P1.
{"title":"Production of antibiotics by Epidermophyton floccosum. 5. Relation between the \"epidermophyton factor\" (EPF) and some steroid antibiotics, particularly fucidin.","authors":"A. Wallerström","doi":"10.1111/J.1699-0463.1970.TB04319.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04319.X","url":null,"abstract":"The “Epidermophyton factor” (EPF) was formed best on cultivation in medium where the reaction was maintained alkaline. The substance gave the chemical colour reactions expected of a steroid. In these reactions and in comparative chromatography EPF closely resembled fucidin, but it was found to be a less acid substance. Nor was EPF identical with 3-keto-fusidic acid, helvolic acid or cephalosporin P1.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"36 1","pages":"395-400"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90149272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04362.X
Sorensen Sf, Nielsen Ls
{"title":"The genetic basis for reactivity in human mixed lymphocyte culture. 2. Studies of unrelated subjects identical at the HL-A subloci \"LA\" and \"Four\".","authors":"Sorensen Sf, Nielsen Ls","doi":"10.1111/J.1699-0463.1970.TB04362.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04362.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"23 1","pages":"719-725"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85505700","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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