Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02365.X
N. Høiby
The occurrence of Pseudomonas aeruginosa in the respiratory tract of 70 cystic fibrosis patients and the occurrence of precipitins against Ps. aeruginosa in sera from the same patients have been investigated during one year by means of monthly bacteriological examinations of tracheal secretions and by means of crossed Immunoelectrophoresis of a polyvalent Ps. aeruginosa antigen against sera from the patients. The one-year period prevalence rate of patients harbouring Ps. aeruginosa was 64 per cent. In newly colonized patients and in intermittently colonized patients non-mucoid strains were predominating, whereas mucoid strains were predominating in chronically colonized patients. The occurrence of mucoid strains, especially in chronically colonized patients, was associated with a significantly higher number of precipitins against Ps. aeruginosa than the occurrence of non-mucoid strains. Males chronically colonized with mucoid strains presented a significantly higher number of precipitins than females chronically colonized with mucoid strains and the number of precipitins was correlated with the duration of the chronic colonization with Ps. aeruginosa in males in contrast to females. The results are in accordance with the hypothesis that mucoid substance could be a virulence factor because it might inhibit the opsonizing effect of the precipitins on the mucoid cells and the complement dependent lysis of the cells hereby favouring mucoid strains at the expense of non-mucoid strains in the respiratory tract of cystic fibrosis patients.
{"title":"Pseudomonas aeruginosa infection in cystic fibrosis. Relationship between mucoid strains of Pseudomonas aeruginosa and the humoral immune response.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1974.TB02365.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02365.X","url":null,"abstract":"The occurrence of Pseudomonas aeruginosa in the respiratory tract of 70 cystic fibrosis patients and the occurrence of precipitins against Ps. aeruginosa in sera from the same patients have been investigated during one year by means of monthly bacteriological examinations of tracheal secretions and by means of crossed Immunoelectrophoresis of a polyvalent Ps. aeruginosa antigen against sera from the patients. The one-year period prevalence rate of patients harbouring Ps. aeruginosa was 64 per cent. In newly colonized patients and in intermittently colonized patients non-mucoid strains were predominating, whereas mucoid strains were predominating in chronically colonized patients. The occurrence of mucoid strains, especially in chronically colonized patients, was associated with a significantly higher number of precipitins against Ps. aeruginosa than the occurrence of non-mucoid strains. Males chronically colonized with mucoid strains presented a significantly higher number of precipitins than females chronically colonized with mucoid strains and the number of precipitins was correlated with the duration of the chronic colonization with Ps. aeruginosa in males in contrast to females. The results are in accordance with the hypothesis that mucoid substance could be a virulence factor because it might inhibit the opsonizing effect of the precipitins on the mucoid cells and the complement dependent lysis of the cells hereby favouring mucoid strains at the expense of non-mucoid strains in the respiratory tract of cystic fibrosis patients.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"58 1","pages":"551-558"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84742192","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02349.X
F. Olsen
By means of the leucocyte migration technique it is demonstrated that the migration indices from 9 patients suffering from essential hypertension are separated significantly from 14 patients suffering from secondary hypertension, diabetic angiopathy, periarteritis nodosa, temporal arteritis, endarteritis obliterans and from 11 normal persons. This means that a simple secondary degenerative damage of the vascular bed is not sufficient to produce measurable cellular antibodies against the vascular wall; accordingly the positive finding in patients with essential hypertension seems to be specific and possibly an important pathogenetic factor in at least some cases of essential hypertension.
{"title":"Delayed hypersensitivity and essential hypertension. Delayed hypersensitivity against arterial wall components as a pathogenetic factor in essential hypertension.","authors":"F. Olsen","doi":"10.1111/J.1699-0463.1974.TB02349.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02349.X","url":null,"abstract":"By means of the leucocyte migration technique it is demonstrated that the migration indices from 9 patients suffering from essential hypertension are separated significantly from 14 patients suffering from secondary hypertension, diabetic angiopathy, periarteritis nodosa, temporal arteritis, endarteritis obliterans and from 11 normal persons. This means that a simple secondary degenerative damage of the vascular bed is not sufficient to produce measurable cellular antibodies against the vascular wall; accordingly the positive finding in patients with essential hypertension seems to be specific and possibly an important pathogenetic factor in at least some cases of essential hypertension.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"34 1","pages":"448-50"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88399297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04300.X
F. Ørskov, I. Ørskov
{"title":"IMMUNOELECTROPHORETIC PATTERNS OF EXTRACTS FROM ALL ESCHERICHIA COLI O AND K ANTIGEN TEST STRAINS","authors":"F. Ørskov, I. Ørskov","doi":"10.1111/J.1699-0463.1970.TB04300.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04300.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"1 1","pages":"263-264"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72642378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04346.X
I. Ørskov, F. Ørskov
Re-examination and re-evaluation of three representative Escherichia coli K antigens of the L variety have been carried out (K12, K51 and K52). It is concluded that the K antigen (L) as hitherto described is not uniform, since more K antigen determinants exist on the surface. Some of these are truly thermolabile, probably proteins, while others are thermostable and are eluted into the suspension medium during heating. This elution is rarely complete. The complex character of the K antigens cannot be detected in the traditional bacterial agglutination technique, but can be demonstrated in double diffusion in gel and immuno-electrophoresis and by passive haemagglutination. The implications for K antigen typing are discussed.
{"title":"The K antigens of Escherichia coli. Re-examination and re-evaluation of the nature of L antigens.","authors":"I. Ørskov, F. Ørskov","doi":"10.1111/J.1699-0463.1970.TB04346.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04346.X","url":null,"abstract":"Re-examination and re-evaluation of three representative Escherichia coli K antigens of the L variety have been carried out (K12, K51 and K52). It is concluded that the K antigen (L) as hitherto described is not uniform, since more K antigen determinants exist on the surface. Some of these are truly thermolabile, probably proteins, while others are thermostable and are eluted into the suspension medium during heating. This elution is rarely complete. The complex character of the K antigens cannot be detected in the traditional bacterial agglutination technique, but can be demonstrated in double diffusion in gel and immuno-electrophoresis and by passive haemagglutination. The implications for K antigen typing are discussed.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"310 1","pages":"593-604"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76453808","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02322.X
Jon Ørstavik
Mouse P-388 cells were grown in suspension culture and exposed to 3H-labelled mitomycin C for 1 hour. With concentrations from 0.5 to 50 μg of 3H-mitomycin C per ml cell culture, about 0.01 per cent of the radioactivity added was found in washed cells. Most of the intracellular antibiotic was present as cold acid soluble material, but significant amounts of tritium were found in the nucleic acid- and protein fractions. The ratio of 3H per weight unit was of the same order of magnitude for DNA and RNA, but substantially lower for cell protein. Double labelling experiments using 14C-thymidine and 3H-mitomycin C were carried out to study the specific loss of antibiotic from the cells during post-treatment incubation. Cold TCA soluble 3H-mitomycin C was rapidly released from the cells during the first hours after they had been transferred to non-radioactive growth medium. Loss of 3H-radioactivity from the RNA- and protein fractions roughly paralleled the loss of 14C radioactivity from the DNA fraction over a period of 3 days. The content of 3H-radioactivity in the DNA containing cell fraction increased during the first day of post-treatment incubation, followed by a substantial drop during the second day. This drop was far more extensive than the corresponding loss of 14C radioactivity. During the third day after exposure to mitomycin C the drops in 3H and 14C in the DNA fraction were not significantly different.
{"title":"UPTAKE AND RELEASE OF 3H‐MITOMYCIN C IN MOUSE P‐388 CELL CULTURES","authors":"Jon Ørstavik","doi":"10.1111/J.1699-0463.1974.TB02322.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02322.X","url":null,"abstract":"Mouse P-388 cells were grown in suspension culture and exposed to 3H-labelled mitomycin C for 1 hour. With concentrations from 0.5 to 50 μg of 3H-mitomycin C per ml cell culture, about 0.01 per cent of the radioactivity added was found in washed cells. Most of the intracellular antibiotic was present as cold acid soluble material, but significant amounts of tritium were found in the nucleic acid- and protein fractions. The ratio of 3H per weight unit was of the same order of magnitude for DNA and RNA, but substantially lower for cell protein. Double labelling experiments using 14C-thymidine and 3H-mitomycin C were carried out to study the specific loss of antibiotic from the cells during post-treatment incubation. Cold TCA soluble 3H-mitomycin C was rapidly released from the cells during the first hours after they had been transferred to non-radioactive growth medium. Loss of 3H-radioactivity from the RNA- and protein fractions roughly paralleled the loss of 14C radioactivity from the DNA fraction over a period of 3 days. The content of 3H-radioactivity in the DNA containing cell fraction increased during the first day of post-treatment incubation, followed by a substantial drop during the second day. This drop was far more extensive than the corresponding loss of 14C radioactivity. During the third day after exposure to mitomycin C the drops in 3H and 14C in the DNA fraction were not significantly different.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"43 1","pages":"270-276"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78581308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04313.X
S. Jyssum, K. Jyssum
{"title":"Endogenous incorporation of 32P in Neisseria meningitidis. 2. The effects of acetate, succinate, propionate and acetoacetate.","authors":"S. Jyssum, K. Jyssum","doi":"10.1111/J.1699-0463.1970.TB04313.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04313.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"2 1","pages":"343-9"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82397125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02364.X
N. Høiby
Seventy patients with cystic fibrosis treated as out-patients in a cystic fibrosis clinic have been followed during one year by monthly bacteriological examinations of tracheal secretions. The daily impression obtained in the laboratory is expressed by the mean point prevalence rate of Pseudomonas aeruginosa: 44 per cent, Staphylococcus aureus: 39 per cent, Haemophilus influenzae: 17 per cent, Diplococcus pneumoniae: 8 per cent, and miscellaneous other bacteria, mainly Enterobacteriaceae: 8 per cent. The fluctuations of the bacteriology are described by additional epidemiological terms: At one or more examinations during the study (period prevalence rate), 90 per cent of the patients harboured St. aureus, 64 per cent Ps. aeruginosa (mainly mucoid strains), 64 per cent H. influenzae, 37 per cent D. pneumoniae, and 30 per cent miscellaneous other bacteria, mainly Enterobacteriaceae. This pattern was found in all age groups with minor age-dependent modifications, especially as regards Enterobacteriaceae. Ps. aeruginosa was predominating as regards chronic colonization which reflects the most difficult therapeutic problems, the period prevalence rate being 39 per cent in contrast to St. aureus: 10 per cent, and H. influenzae: 1 per cent. St. aureus was predominating as regards new colonization and recolonization which reflect the problems of prevention, the incidence rate of new colonization and recolonization per risk group being 84 per cent, followed by H. influenzae: 62 per cent, Ps. aeruginosa: 43 per cent, and D. pneumoniae: 30 per cent. The results show that the bacteriological problems in cystic fibrosis are still considerable as regards therapy as well as prevention. Although many species may colonize the respiratory tracts of these patients, the main clinical problems concern Ps. aeruginosa and St. aureus; the reason why is discussed.
{"title":"Epidemiological investigations of the respiratory tract bacteriology in patients with cystic fibrosis.","authors":"N. Høiby","doi":"10.1111/J.1699-0463.1974.TB02364.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02364.X","url":null,"abstract":"Seventy patients with cystic fibrosis treated as out-patients in a cystic fibrosis clinic have been followed during one year by monthly bacteriological examinations of tracheal secretions. The daily impression obtained in the laboratory is expressed by the mean point prevalence rate of Pseudomonas aeruginosa: 44 per cent, Staphylococcus aureus: 39 per cent, Haemophilus influenzae: 17 per cent, Diplococcus pneumoniae: 8 per cent, and miscellaneous other bacteria, mainly Enterobacteriaceae: 8 per cent. The fluctuations of the bacteriology are described by additional epidemiological terms: At one or more examinations during the study (period prevalence rate), 90 per cent of the patients harboured St. aureus, 64 per cent Ps. aeruginosa (mainly mucoid strains), 64 per cent H. influenzae, 37 per cent D. pneumoniae, and 30 per cent miscellaneous other bacteria, mainly Enterobacteriaceae. This pattern was found in all age groups with minor age-dependent modifications, especially as regards Enterobacteriaceae. Ps. aeruginosa was predominating as regards chronic colonization which reflects the most difficult therapeutic problems, the period prevalence rate being 39 per cent in contrast to St. aureus: 10 per cent, and H. influenzae: 1 per cent. St. aureus was predominating as regards new colonization and recolonization which reflect the problems of prevention, the incidence rate of new colonization and recolonization per risk group being 84 per cent, followed by H. influenzae: 62 per cent, Ps. aeruginosa: 43 per cent, and D. pneumoniae: 30 per cent. The results show that the bacteriological problems in cystic fibrosis are still considerable as regards therapy as well as prevention. Although many species may colonize the respiratory tracts of these patients, the main clinical problems concern Ps. aeruginosa and St. aureus; the reason why is discussed.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"50 1","pages":"541-50"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85053584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1970.TB04278.X
G. Kronvall
{"title":"PROTEIN A – AN “INCOMPLETE” STAPHYLOCOCCAL AGGLUTINOGEN","authors":"G. Kronvall","doi":"10.1111/J.1699-0463.1970.TB04278.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1970.TB04278.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"9 1","pages":"127-128"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84633026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02376.X
E. Jantzen, T. Bergan, K. Bøvre
{"title":"Gas chromatography of bacterial whole cell methanolysates; VI. Fatty acid composition of strains within Micrococcaceae;.","authors":"E. Jantzen, T. Bergan, K. Bøvre","doi":"10.1111/J.1699-0463.1974.TB02376.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02376.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"19 1","pages":"785-798"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83001297","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2009-08-15DOI: 10.1111/J.1699-0463.1974.TB02358.X
K. Hougen
Treponema phagedenis, Treponema vincentii, and Treponema refringens were studied in the electron microscope by means of negative staining and sectioning techniques. The length and width of the cells were within the same limits for all three strains examined. Statistical analysis, however, demonstrated a difference in the wavelengths of the cells. Organisms of T. phagedenis and T. vincentii had blunt ends and possessed cell wall surface layers in which a regular substructure was only occasionally revealed, while cells of T. refringens had tapered ends and a distinctly structured cell wall surface layer. Generally, cells of the strains investigated had 4–6 flagella inserted at each end. Two bundles of flagella, one from each end of the cell, wound around the organism and overlapped in the middle of the cell. The flagella with their insertion organelles were identical for the three strains studied. Two bundles of cytoplasmic tubules were detected in the interior of the treponemes after treatment with either sodium deoxycholate or Myxobacter AL-1 protease 1. The two bundles of cytoplasmic tubules overlapped in the middle of the cell. The results of the present morphological study are compared with results obtained by other investigators studying other characteristics of these strains and it is concluded that the three strains studied belong to three different species.
{"title":"The ultrastructure of cultivable treponemes. 2. Treponema calligyrum, Treponema minutum and Treponema microdentium.","authors":"K. Hougen","doi":"10.1111/J.1699-0463.1974.TB02358.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02358.X","url":null,"abstract":"Treponema phagedenis, Treponema vincentii, and Treponema refringens were studied in the electron microscope by means of negative staining and sectioning techniques. The length and width of the cells were within the same limits for all three strains examined. Statistical analysis, however, demonstrated a difference in the wavelengths of the cells. Organisms of T. phagedenis and T. vincentii had blunt ends and possessed cell wall surface layers in which a regular substructure was only occasionally revealed, while cells of T. refringens had tapered ends and a distinctly structured cell wall surface layer. Generally, cells of the strains investigated had 4–6 flagella inserted at each end. Two bundles of flagella, one from each end of the cell, wound around the organism and overlapped in the middle of the cell. The flagella with their insertion organelles were identical for the three strains studied. Two bundles of cytoplasmic tubules were detected in the interior of the treponemes after treatment with either sodium deoxycholate or Myxobacter AL-1 protease 1. The two bundles of cytoplasmic tubules overlapped in the middle of the cell. The results of the present morphological study are compared with results obtained by other investigators studying other characteristics of these strains and it is concluded that the three strains studied belong to three different species.","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"40 1","pages":"495-507"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88652724","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
京公网安备 11010802042870号
京ICP备2023020795号-1
扫码关注我们
求助内容：
应助结果提醒方式：