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Canine mycoplasmas II: biochemical characterization and serological identification. 犬支原体II型:生化特性和血清学鉴定。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB02288.X
S. Rosendal
A total of 118 mycoplasma strains isolated from the conjunctivae and the respiratory- and genital tracts of 78 dogs were studied biochemically and serologically. Three biochemical tests (glucose fermentation, catabolism of arginine and phosphatase activity) were found to be very useful for a preliminary biochemical grouping prior to serological identification. Using these tests, the strains could be divided into six groups that did not show any antigenic overlapping. By serological examination using the indirect immunofluorescence technique and growth inhibition 35 isolates were identified as M. canis; 21 were identified as M. edwardii; 10 as M. cynos; 10 as the feline species M. gateae; 30 as M. spumans; 2 as M. maculosum; 2 as the bovine species M. bovigenitalium; and 5 as M. feliminutum, a species previously represented by a single strain isolated from a cat. The three remaining strains which were glucose positive and arginine and phosphatase negative were placed in a separate serogroup, group A, as they were serologically different from the established canine species.
对78只犬结膜、呼吸道和生殖道分离的118株支原体进行了生化和血清学研究。三个生化测试(葡萄糖发酵,精氨酸分解代谢和磷酸酶活性)被发现对血清学鉴定之前的初步生化分组非常有用。通过这些测试,这些菌株可以分为六组,没有显示任何抗原重叠。采用间接免疫荧光技术和生长抑制技术进行血清学检测,鉴定35株分离株为犬支原体;鉴定为爱德华氏分枝杆菌21株;10 . M. cynos;10种为猫科动物门门门门;30例为M. spumans;2 . M. maculosum;2为牛种M. bovigenitalium;5个为猫分枝杆菌,该物种以前由从猫身上分离出来的单一菌株代表。其余三株葡萄糖阳性,精氨酸和磷酸酶阴性的菌株被放置在一个单独的血清组,a组,因为它们在血清学上与已建立的犬种不同。
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引用次数: 9
Pseudomonas aeruginosa infection in cystic fibrosis. Distribution of B and T lymphocytes in relation to the humoral immune response. 囊性纤维化中的铜绿假单胞菌感染。B淋巴细胞和T淋巴细胞分布与体液免疫反应的关系。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB02366.X
N. Høiby, L. Mathiesen
The influence of chronic Ps. aeruginosa infection on the occurrence of bone marrow-derived lymphocytes (B cells) and thymus-derived lymphocytes (T cells) in peripheral blood have been studied in 2 groups of patients with cystic fibrosis. One group (9 patients) suffered from chronic Ps. aeruginosa infection in the respiratory tract and produced multiple Ps. aeruginosa precipitins which were demonstrated by means of crossed immunoelectrophoresis. The other group (9 patients) had never harboured Ps. aeruginosa in the respiratory tract and presented no demonstrable precipitins against this bacteria. The lymphocytes were examined for the presence of 2 surface markers for B cells: receptor for C3 complement component (EAC rosette-formation) and surface immunoglobulins (immunofluorescent staining), and for the presence of 1 surface marker for T cells: spontaneous binding of sheep red blood cells (E rosette-formation). The B:T cell ratio was nearly identical in the 2 groups of patients whereas the total numbers of B and T cells were significant higher in patients with chronic Ps. aeruginosa infection. The number of Ps. aeruginosa precipitins per serum was significant positively correlated to the total number of T cells, wheras the correlation with the total number of B cells was insignificant.
研究慢性铜绿假单胞菌感染对2组囊性纤维化患者外周血骨髓源性淋巴细胞(B细胞)和胸腺源性淋巴细胞(T细胞)发生的影响。其中一组(9例)呼吸道慢性铜绿假单胞菌感染,产生多种铜绿假单胞菌沉淀,经交叉免疫电泳证实。另一组(9例)从未在呼吸道中存在铜绿假单胞菌,也没有明显的针对该细菌的沉淀。检测淋巴细胞是否存在2种B细胞表面标记物:C3补体成分受体(EAC莲座形成)和表面免疫球蛋白(免疫荧光染色);检测T细胞表面标记物:羊红细胞自发结合(E莲座形成)。两组患者的B:T细胞比例几乎相同,而慢性铜绿假单胞菌感染患者的B细胞和T细胞总数明显较高。血清中铜绿假单胞菌沉淀蛋白的数量与T细胞总数呈显著正相关,与B细胞总数的相关性不显著。
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引用次数: 12
Leucocyte migration test in agarose. The use of puromycin in disclosure of non-immunological inhibition. 琼脂糖中白细胞迁移试验。使用嘌呤霉素揭露非免疫抑制。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB02323.X
K. Mygind, S. Stenbjerg
The response of peripheral human leucocytes to PPD and tubercle bacilli was studied in the leucocyte migration test in agarose medium. At PPD 50 μg/ml migration of leucocytes from tuberculin positive persons was inhibited in all cases tested, while no migration inhibition of cells from tuberculin negative persons was found. There was no overlapping between the MI of the two groups. Blocking of protein synthesis by means of puromycin prevented the migration inhibition of cells from tuberculin positive persons at PPD 50 μg/ml and 100 μg/ml. In some cases the migration of cells from tuberculin negative persons was inhibited in the presence of PPD 100 μg/ml. This inhibition could not be prevented by puromycin and was probably due to a toxic effect of PPD. It is suggested that puromycin may be useful in dislocating false, probably toxic, inhibition.
通过琼脂糖培养基白细胞迁移试验,研究了人外周血白细胞对PPD和结核杆菌的反应。在PPD 50 μg/ml时,结核菌素阳性者白细胞的迁移均受到抑制,而结核菌素阴性者白细胞的迁移未见抑制。两组心肌梗死无重叠。在PPD为50 μg/ml和100 μg/ml时,嘌呤霉素阻断蛋白合成可阻止结核菌素阳性细胞的迁移抑制。在某些情况下,结核菌素阴性者细胞的迁移在PPD 100 μg/ml的存在下受到抑制。这种抑制不能被嘌呤霉素阻止,可能是由于PPD的毒性作用。提示嘌呤霉素可用于脱臼、假的、可能有毒的抑制。
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引用次数: 5
Proteolytic enzymes and biological inhibitors. 3. Naturally occurring inhibitors in some animal and plant materials and their effect upon proteolytic enzymes of various origin. 蛋白水解酶和生物抑制剂。3.一些动物和植物材料中天然存在的抑制剂及其对各种来源的蛋白水解酶的影响。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1970.TB04365.X
K. Fossum
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引用次数: 8
Bone marrow transplantation in inbred strains of mice. I. The failure of development of normal T cell function following allogeneic transplantation. 近交系小鼠的骨髓移植。1 .同种异体移植后正常T细胞功能发育的失败。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB00242.X
L. Trier, B. Rubin
Lethally irradiated mice reconstituted with allogeneic bone marrow cells usually survive the irradiation damage, but their immune-status is not completely restored. Thus, such mice show depressed humoral antibody responses to thymus dependent antigens, prolonged allogeneic skin graft survival, and their spleen cells display reduced graft-versus-host (GVH) activity when inoculated into newborn F1 hybrid mice. The present study was carried out in order to explore the reason(s) for the depressed immunological activity of allogeneic irradiation chimaeric mice. It was found that these mice had an elevated level of θ positive cells compared to mice transplanted with syngeneic bone marrow cells, but their spleen cells did not show any detectable specific or third-party GVH response. Spleen cells from allogeneic irradiation chimaeras were not able to participate in a collaborative GVH response with lymph node cells either. Possible reasons for the immunological inertness of θ positive cells in allogeneic irradiation chimaeras are discussed.
用同种异体骨髓细胞重建的致死性辐照小鼠通常能在辐照损伤中存活,但其免疫状态不能完全恢复。因此,这些小鼠对胸腺依赖抗原的体液抗体反应降低,异体皮肤移植存活时间延长,其脾脏细胞接种到新生F1杂交小鼠后显示移植物抗宿主(GVH)活性降低。本研究旨在探讨同种异体辐照嵌合小鼠免疫活性下降的原因。结果发现,与移植同种骨髓细胞的小鼠相比,这些小鼠的θ阳性细胞水平升高,但它们的脾脏细胞没有表现出任何可检测到的特异性或第三方GVH反应。同种异体辐照嵌合体的脾脏细胞也不能参与与淋巴结细胞的协同GVH反应。讨论了异体辐照嵌合体中θ阳性细胞免疫惰性的可能原因。
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引用次数: 6
Proteolytic enzymes and biological inhibitors. II. Naturally occurring inhibitors in sera from different species and their effect upon proteolytic enzymes of various origin. 蛋白水解酶和生物抑制剂。2不同物种血清中天然存在的抑制剂及其对不同来源的蛋白水解酶的影响。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1970.TB04347.X
Kåre Fossum
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引用次数: 32
Experimental toxoplasmosis in pregnant sows. I. Clinical, parasitological and serological observations. 妊娠母猪实验性弓形虫病。1 .临床、寄生虫学和血清学观察。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1970.TB04279.X
K. Work, L. Eriksen, K. L. Fennestad, T. Moller, J. Siim
Six sows in the 3rd month of gestation were infected with Toxoplasma gondii, four intravenously with the RH strain, two perorally with a porcine strain. Severe disease, characterized by fever, anorexia and dyspnoea, was seen in the animals infected with the RH strain and one sow died on day 4. Mild febrile disease was seen in the porcine strain infections. Parasitaemia and Toxoplasma antibodies became demonstrable in all sows, and at term the sows delivered dead and/or live piglets, the mortality being 50–100 per cent in three RH strain infected litters and 30 per cent in one porcine strain infected litter. At sacrifice, Toxoplasma was found in the sows infected with the porcine strain but in none of those infected with the RH strain. Non-viable Toxoplasma was demonstrated histologically in 17 of 35 dead piglets and viable Toxoplasma was isolated from the cord blood of three liveborn piglets. At sacrifice 3–4 weeks after birth, Toxoplasma was demonstrable in several piglets from both sows infected with the porcine strain but in none of the piglets from sows infected with the RH strain. It is concluded that toxoplasmosis during the 3rd month of pregnancy in swine may cause fetal infection, resulting in death and congenital toxoplasmosis of liveborn piglets. Infection of the fetuses occurs probably during the maternal parasitaemia but horizontal infection i. e. from one litter mate to another, may also take place. There is little chance of isolating viable Toxoplasma from mummified or macerated fetuses, but non-viable Toxoplasma may be demonstrated by histological examination.
6头妊娠3个月的母猪感染了刚地弓形虫,其中4头经静脉注射感染RH株,2头经口感染猪株。感染RH菌株的动物出现严重疾病,表现为发热、厌食和呼吸困难,1头母猪在第4天死亡。在猪株感染中可见轻度发热病。在所有母猪中均可发现寄生虫病和弓形虫抗体,并且在足月母猪分娩死仔猪和/或活仔猪,三个RH株感染窝的死亡率为50 - 100%,一个猪株感染窝的死亡率为30%。在牺牲时,在感染猪株的母猪中发现弓形虫,但在感染RH株的母猪中没有发现弓形虫。35头死仔猪中有17头在组织学上证实无活的弓形虫,并从3头活产仔猪的脐带血中分离出有活的弓形虫。在出生后3-4周的牺牲时,在感染猪株的母猪的几头仔猪中可以发现弓形虫,但在感染RH株的母猪的仔猪中没有发现弓形虫。由此可见,猪妊娠3个月时感染弓形虫病可引起胎儿感染,导致仔猪死亡和先天性弓形虫病。胎儿的感染可能发生在母体寄生虫血症期间,但也可能发生水平感染,即从一胎配偶传染给另一胎配偶。从干尸或浸渍的胎儿中分离出有活力的弓形虫的可能性很小,但组织学检查可能会证明没有活力的弓形虫。
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引用次数: 24
The effect of different serum concentrations of antimicrobial agents on the Lactobacillus casei folate assay. 不同血清浓度抗菌药物对干酪乳杆菌叶酸测定的影响。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB02339.X
G. Asbjørnsen
{"title":"The effect of different serum concentrations of antimicrobial agents on the Lactobacillus casei folate assay.","authors":"G. Asbjørnsen","doi":"10.1111/J.1699-0463.1974.TB02339.X","DOIUrl":"https://doi.org/10.1111/J.1699-0463.1974.TB02339.X","url":null,"abstract":"","PeriodicalId":7323,"journal":{"name":"Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology","volume":"16 1","pages":"371-4"},"PeriodicalIF":0.0,"publicationDate":"2009-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79279172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Proteolytic enzymes and biological inhibitors. IV. Bacterial proteinase inhibitors and their effect upon enzymes of various origin. 蛋白水解酶和生物抑制剂。细菌蛋白酶抑制剂及其对各种来源的酶的影响。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1970.TB04366.X
K. Fossum
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引用次数: 14
Purine metabolism in Neisseria meningitidis. 2. Utilization of exogenous adenosine, guanosine and inosine. 脑膜炎奈瑟菌嘌呤代谢。2. 外源性腺苷、鸟苷和肌苷的利用。
Pub Date : 2009-08-15 DOI: 10.1111/J.1699-0463.1974.TB02387.X
S. Jyssum
Whole cells of Neisseria meningitidis are found to utilize exogenous radioactive adenosine with labelling of the adenine, adenosine 5′-monophosphate, adenosine 5′-diphosphate and adenosine 5′-triphosphate pools. Extracts from N. meningitidis were found to have activities corresponding to adenosine nucleosidase (EC 3.2.2.7). No activities corresponding to purinenucleoside phosphorylase (EC 2.4.2.1), adenosine kinase (EC 2.7.1.20) or adenosine deaminase (EC 3.5.4.4) could be demonstrated. Radioactive guanosine or inosine were not found to be utilized by whole cells. A hydrolytic breakdown of guanosine or inosine to guanine and ribose, respectively hypoxanthine and ribose could not be demonstrated in crude extracts. Likewise, the search for activities corresponding to a guanosine kinase or to inosine kinase (EC 2.7.1.73) was altogether negative.
发现整个脑膜炎奈瑟菌细胞利用外源性放射性腺苷标记腺嘌呤、5′-单磷酸腺苷、5′-二磷酸腺苷和5′-三磷酸腺苷池。发现脑膜炎奈瑟菌提取物具有相应的腺苷核苷酶活性(EC 3.2.2.7)。嘌呤核苷磷酸化酶(EC 2.4.2.1)、腺苷激酶(EC 2.7.1.20)和腺苷脱氨酶(EC 3.5.4.4)均无相应活性。放射性鸟苷或肌苷未被整个细胞利用。在粗提取物中,鸟苷或肌苷水解分解为鸟嘌呤和核糖,分别为次黄嘌呤和核糖。同样,与鸟苷激酶或肌苷激酶(EC 2.7.1.73)相对应的活性搜索完全为阴性。
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引用次数: 4
期刊
Acta pathologica et microbiologica Scandinavica. Section B: Microbiology and immunology
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