Pub Date : 2025-08-25DOI: 10.1016/j.jciso.2025.100145
Margaux Ceccaldi , Coralie Menuisier , Vincent Langlois , Marielle Guéguen , Yoan Péchaud , Daniel Grande , Sébastien Vincent-Bonnieu , Olivier Pitois
Building on the development history of the Microbially Induced Calcite Precipitation (MICP) method and recent insights into the organization of liquid and bubbles within pore spaces filled with liquid foam, we hypothesize a potential synergy between MICP and liquid foam for enhancing soil properties. We assess the respiration and calcification capabilities of the bacterium Sporosarcina pasteurii in various foaming solutions formulated with bio-based surfactants. The most promising formulations are then used for in-situ observations of bacteria-induced calcification within foam-embedded 2D granular packings. We observe that maltoside-type AlkylPolyGlucosides with relatively short chains, in particular, enable optimal calcification in less than an hour. In the granular packing, the foam spontaneously creates liquid zones enriched with bacteria at the inter-grain contacts. As a result, instead of being distributed across the entire porous space, calcification is localized in these zones, promoting the formation of solid bridges (CaCO). This suggests that the foam-based MICP method could represent a relevant variation of the original approach, though this remains to be confirmed at larger scales through mechanical testing on representative soil samples.
{"title":"Foam-based microbially-induced calcite precipitation","authors":"Margaux Ceccaldi , Coralie Menuisier , Vincent Langlois , Marielle Guéguen , Yoan Péchaud , Daniel Grande , Sébastien Vincent-Bonnieu , Olivier Pitois","doi":"10.1016/j.jciso.2025.100145","DOIUrl":"10.1016/j.jciso.2025.100145","url":null,"abstract":"<div><div>Building on the development history of the Microbially Induced Calcite Precipitation (MICP) method and recent insights into the organization of liquid and bubbles within pore spaces filled with liquid foam, we hypothesize a potential synergy between MICP and liquid foam for enhancing soil properties. We assess the respiration and calcification capabilities of the bacterium Sporosarcina pasteurii in various foaming solutions formulated with bio-based surfactants. The most promising formulations are then used for in-situ observations of bacteria-induced calcification within foam-embedded 2D granular packings. We observe that maltoside-type AlkylPolyGlucosides with relatively short chains, in particular, enable optimal calcification in less than an hour. In the granular packing, the foam spontaneously creates liquid zones enriched with bacteria at the inter-grain contacts. As a result, instead of being distributed across the entire porous space, calcification is localized in these zones, promoting the formation of solid bridges (CaCO<span><math><msub><mrow></mrow><mrow><mn>3</mn></mrow></msub></math></span>). This suggests that the foam-based MICP method could represent a relevant variation of the original approach, though this remains to be confirmed at larger scales through mechanical testing on representative soil samples.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100145"},"PeriodicalIF":0.0,"publicationDate":"2025-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144917670","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-23DOI: 10.1016/j.jciso.2025.100148
Nasser A.M. Barakat, Aghareed M. Tayeb, Rahma Hamad, Rasha A. Hefny
Photocatalytic methanol photo-reforming stands as a promising avenue for sustainable hydrogen production, with potential applications in clean energy generation. In this study, we investigate the utilization of CoO/Co3O4 nanofibers as a photocatalyst for methanol photo-reforming, evaluating its performance under different reaction conditions. The synthesized nanofibers are employed with both pure methanol and methanol/water mixtures and compared against TiO2 nanoparticles for reference. Gas evolution rates and composition are analyzed to elucidate the catalytic behavior of the nanofibers. Results reveal that the CoO/Co3O4 nanofibers exhibit superior catalytic activity, yielding high gas evolution rates with pure methanol; 600 mmol/gcat·s. However, the addition of water leads to a sharp decrease in gas production rate (66.9 mmol/gcat.s), attributed to competitive water adsorption on the catalyst surface and drastic change in the reaction kinetics. Gas analysis demonstrates the predominant production of carbon monoxide and hydrogen with pure methanol, while carbon dioxide is detected in the presence of water. In contrast, TiO2 nanoparticles primarily yield carbon dioxide and hydrogen, with no carbon monoxide detected. These findings provide valuable insights into the catalytic mechanisms and selectivity of nanofiber-based catalysts for methanol photo-reforming, paving the way for the development of efficient and selective photocatalytic systems for sustainable energy production.
{"title":"Visible-light-driven methanol photo-reforming over cobalt oxides nanofibers: Insights into synthesis, bandgap engineering, and catalytic efficiency","authors":"Nasser A.M. Barakat, Aghareed M. Tayeb, Rahma Hamad, Rasha A. Hefny","doi":"10.1016/j.jciso.2025.100148","DOIUrl":"10.1016/j.jciso.2025.100148","url":null,"abstract":"<div><div>Photocatalytic methanol photo-reforming stands as a promising avenue for sustainable hydrogen production, with potential applications in clean energy generation. In this study, we investigate the utilization of CoO/Co<sub>3</sub>O<sub>4</sub> nanofibers as a photocatalyst for methanol photo-reforming, evaluating its performance under different reaction conditions. The synthesized nanofibers are employed with both pure methanol and methanol/water mixtures and compared against TiO<sub>2</sub> nanoparticles for reference. Gas evolution rates and composition are analyzed to elucidate the catalytic behavior of the nanofibers. Results reveal that the CoO/Co<sub>3</sub>O<sub>4</sub> nanofibers exhibit superior catalytic activity, yielding high gas evolution rates with pure methanol; 600 mmol/g<sub>cat</sub>·s. However, the addition of water leads to a sharp decrease in gas production rate (66.9 mmol/g<sub>cat</sub>.s), attributed to competitive water adsorption on the catalyst surface and drastic change in the reaction kinetics. Gas analysis demonstrates the predominant production of carbon monoxide and hydrogen with pure methanol, while carbon dioxide is detected in the presence of water. In contrast, TiO<sub>2</sub> nanoparticles primarily yield carbon dioxide and hydrogen, with no carbon monoxide detected. These findings provide valuable insights into the catalytic mechanisms and selectivity of nanofiber-based catalysts for methanol photo-reforming, paving the way for the development of efficient and selective photocatalytic systems for sustainable energy production.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100148"},"PeriodicalIF":0.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A glutathione (GSH)-responsive metal-organic framework (MOF) was developed using biocompatible L-cystine and Zn for targeted doxorubicin (DOX) delivery. This strategy enhances drug release in the microenvironment while minimizing systemic toxicity. Disulfide-containing MOFs were synthesized using L-cystine as the ligand and Zn as the metal node. A one-pot method was developed to encapsulate DOX during synthesis. The redox-responsive behavior of the resulting DOX@MOF-Zn(cystine) was evaluated under physiological and tumor-mimicking conditions. In vitro assays revealed that the disulfide bonds in L-cystine ligand facilitate MOF degradation in the presence of GSH, triggering DOX release. DOX@MOF-Zn(cystine) exhibited significantly enhanced release under conditions mimicking the tumor microenvironment (pH 5.4, 20 mM GSH) compared to physiological conditions (pH 7.4, no GSH). Cell viability assays demonstrated minimal toxicity for blank MOFs and strong DOX-dependent anticancer effects from DOX@MOF-Zn(cystine). These findings suggest DOX@MOF-Zn(cystine) is an effective GSH-responsive drug delivery system for targeted cancer therapy.
采用生物相容性l-胱氨酸和锌制备了谷胱甘肽（GSH）响应型金属-有机骨架（MOF），用于靶向递送阿霉素（DOX）。这种策略增强了药物在微环境中的释放，同时最小化了全身毒性。以l -胱氨酸为配体，锌为金属节点，合成了含二硫化物的mof。在合成过程中，采用一锅法包封DOX。在生理和肿瘤模拟条件下评估所得DOX@MOF-Zn（胱氨酸）的氧化还原反应行为。体外实验表明，l -胱氨酸配体中的二硫键在GSH存在下促进MOF降解，触发DOX释放。与生理条件（pH 7.4，无GSH）相比，DOX@MOF-Zn（胱氨酸）在模拟肿瘤微环境（pH 5.4, 20 mM GSH）下的释放明显增强。细胞活力测试表明，DOX@MOF-Zn（胱氨酸）对空白mof的毒性很小，具有很强的dox依赖性抗癌作用。这些发现表明DOX@MOF-Zn（胱氨酸）是一种有效的gsh反应性药物递送系统，用于靶向癌症治疗。
{"title":"Cancer cell microenvironment-responsive bio-metal-organic frameworks for anticancer drug delivery","authors":"Asuka Inada, Hirona Yoshinaka, Misa Kikuchi, Tatsuya Oshima","doi":"10.1016/j.jciso.2025.100149","DOIUrl":"10.1016/j.jciso.2025.100149","url":null,"abstract":"<div><div>A glutathione (GSH)-responsive metal-organic framework (MOF) was developed using biocompatible L-cystine and Zn for targeted doxorubicin (DOX) delivery. This strategy enhances drug release in the microenvironment while minimizing systemic toxicity. Disulfide-containing MOFs were synthesized using L-cystine as the ligand and Zn as the metal node. A one-pot method was developed to encapsulate DOX during synthesis. The redox-responsive behavior of the resulting DOX@MOF-Zn(cystine) was evaluated under physiological and tumor-mimicking conditions. In vitro assays revealed that the disulfide bonds in L-cystine ligand facilitate MOF degradation in the presence of GSH, triggering DOX release. DOX@MOF-Zn(cystine) exhibited significantly enhanced release under conditions mimicking the tumor microenvironment (pH 5.4, 20 mM GSH) compared to physiological conditions (pH 7.4, no GSH). Cell viability assays demonstrated minimal toxicity for blank MOFs and strong DOX-dependent anticancer effects from DOX@MOF-Zn(cystine). These findings suggest DOX@MOF-Zn(cystine) is an effective GSH-responsive drug delivery system for targeted cancer therapy.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100149"},"PeriodicalIF":0.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144902307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-08-20DOI: 10.1016/j.jciso.2025.100147
Muhammadin Hamid , Indri Dayana , Habib Satria , Dadan Ramdan , Junaidi , Muhammad Fadlan Siregar , Dewi Sholeha , Juliaster Marbun , Hadi Wijoyo
The synthesis of porous hard carbon from coconut biomass waste using physical activation through pyrolysis and chemical activation with orange juice as an organic activator at varying temperatures, including 500 °C, 600 °C, and 700 °C, has been carried out. The purpose of this research is to develop materials for faradaic-type supercapacitor electrodes based on these temperature variations. Characterization and testing methods used include X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). BET analysis showed that the PHC-700 sample exhibited a relatively high specific surface area with porous characteristics and a narrow pore size distribution around 17–18 nm. The PHC-700 sample also exhibited the best electrochemical performance with a specific capacitance of 485.23 F/g and a total resistance of 24.29 Ω. GCD measurements revealed stable triangular curves, indicating good charge-discharge reversibility and confirming the superior capacitive behavior of PHC-700. The study confirmed that the higher the temperature used, the better the supercapacitor electrode obtained. This is evidenced by the pores on the surface that help improve the quality of the electrode and the XRD test results which are directly proportional to the increase in temperature. It is hoped that this research can enhance understanding of supercapacitor electrode applications.
{"title":"Porous hard carbon derived from coconut biomass waste as electrode material for supercapacitor","authors":"Muhammadin Hamid , Indri Dayana , Habib Satria , Dadan Ramdan , Junaidi , Muhammad Fadlan Siregar , Dewi Sholeha , Juliaster Marbun , Hadi Wijoyo","doi":"10.1016/j.jciso.2025.100147","DOIUrl":"10.1016/j.jciso.2025.100147","url":null,"abstract":"<div><div>The synthesis of porous hard carbon from coconut biomass waste using physical activation through pyrolysis and chemical activation with orange juice as an organic activator at varying temperatures, including 500 °C, 600 °C, and 700 °C, has been carried out. The purpose of this research is to develop materials for faradaic-type supercapacitor electrodes based on these temperature variations. Characterization and testing methods used include X-ray diffraction (XRD), scanning electron microscopy (SEM), Brunauer-Emmett-Teller (BET), cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS). BET analysis showed that the PHC-700 sample exhibited a relatively high specific surface area with porous characteristics and a narrow pore size distribution around 17–18 nm. The PHC-700 sample also exhibited the best electrochemical performance with a specific capacitance of 485.23 F/g and a total resistance of 24.29 Ω. GCD measurements revealed stable triangular curves, indicating good charge-discharge reversibility and confirming the superior capacitive behavior of PHC-700. The study confirmed that the higher the temperature used, the better the supercapacitor electrode obtained. This is evidenced by the pores on the surface that help improve the quality of the electrode and the XRD test results which are directly proportional to the increase in temperature. It is hoped that this research can enhance understanding of supercapacitor electrode applications.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100147"},"PeriodicalIF":0.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144890911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The growing demand for sustainable nanomaterial synthesis has driven the development of green, bio-mediated approaches. In this study, CuO-ZnO nanocomposites (CuxZn1−xO; x = 0, 0.025, 0.05, 0.075, and 0.1) were synthesized via a phytochemical using the latex of Jatropha curcas an underexplored natural source as a reducing, capping, and stabilizing agent. This eco-friendly, latex-assisted approach avoids toxic chemicals and harsh synthesis conditions, making it fully compliant with green chemistry principles. The synthesized nanocomposites were systematically characterized to assess their structural and physicochemical properties. X-ray diffraction (XRD) confirmed the formation of a predominant hexagonal wurtzite ZnO phase with a secondary monoclinic CuO phase. Field emission scanning electron microscopy (FE-SEM) revealed quasi-spherical nanoparticles with well-defined facets, while energy-dispersive X-ray spectroscopy (EDS) verified the successful incorporation of Cu and Zn. Zeta potential analysis, UV–Visible spectroscopy, and FT-IR spectroscopy provided further insights into colloidal stability, optical properties and surface functionalities respectively. Among the synthesized materials, Cu0.05Zn0.95O exhibited the highest photocatalytic activity, achieving 88.7 % degradation of methylene blue with a kinetic rate constant of 0.01831 min−1. Reusability tests and scavenger studies confirmed the catalyst's durability and mechanistic reliability. To the best of our knowledge, there are very few or no reports employing Jatropha curcas latex for Cu-doped ZnO synthesis, particularly targeting photocatalytic applications, thereby demonstrating a novel eco-friendly pathway. Overall, this work demonstrates the efficacy of Jatropha-assisted green synthesis for producing stable and efficient CuO-ZnO nanocomposites, offering a promising solution for the photocatalytic treatment of dye-contaminated wastewater.
{"title":"Biogenic CuO-ZnO nanocomposites synthesized from Jatropha curcas for methylene blue dye degradation","authors":"Yojana Sharma , Vikas Anand , Vikas Dhiman , Pawan Heera","doi":"10.1016/j.jciso.2025.100146","DOIUrl":"10.1016/j.jciso.2025.100146","url":null,"abstract":"<div><div>The growing demand for sustainable nanomaterial synthesis has driven the development of green, bio-mediated approaches. In this study, CuO-ZnO nanocomposites (Cu<sub>x</sub>Zn<sub>1−x</sub>O; x = 0, 0.025, 0.05, 0.075, and 0.1) were synthesized via a phytochemical using the latex of <em>Jatropha curcas</em> an underexplored natural source as a <em>reducing, capping,</em> and <em>stabilizing agent</em>. This eco-friendly, latex-assisted approach avoids toxic chemicals and harsh synthesis conditions, making it fully compliant with green chemistry principles. The synthesized nanocomposites were systematically characterized to assess their structural and physicochemical properties. X-ray diffraction (XRD) confirmed the formation of a predominant hexagonal wurtzite ZnO phase with a secondary monoclinic CuO phase. Field emission scanning electron microscopy (FE-SEM) revealed quasi-spherical nanoparticles with well-defined facets, while energy-dispersive X-ray spectroscopy (EDS) verified the successful incorporation of Cu and Zn. Zeta potential analysis, UV–Visible spectroscopy, and FT-IR spectroscopy provided further insights into colloidal stability, optical properties and surface functionalities respectively. Among the synthesized materials, Cu<sub>0.05</sub>Zn<sub>0.95</sub>O exhibited the highest photocatalytic activity, achieving 88.7 % degradation of methylene blue with a kinetic rate constant of 0.01831 min<sup>−1</sup>. Reusability tests and scavenger studies confirmed the catalyst's durability and mechanistic reliability. To the best of our knowledge, there are very few or no reports employing <em>Jatropha curcas</em> latex for Cu-doped ZnO synthesis, particularly targeting photocatalytic applications, thereby demonstrating a novel eco-friendly pathway. Overall, this work demonstrates the efficacy of Jatropha-assisted green synthesis for producing stable and efficient CuO-ZnO nanocomposites, offering a promising solution for the photocatalytic treatment of dye-contaminated wastewater.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100146"},"PeriodicalIF":0.0,"publicationDate":"2025-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144853025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-21DOI: 10.1016/j.jciso.2025.100144
Ratnaningsih Eko Sardjono , Ramdhan Gunawan , Asep Kadarohman , Budiman Anwar , Hamidie Ronald Daniel Ray , Suci Nur Vikasari , Erdiwansyah , Siti Fatimah , Young Gun Ko
Mucuna pruriens (MP), a natural source of L-DOPA, holds potential as a Parkinson's disease (PD) treatment. This study developed two encapsulation systems for MP using nanocellulose (NC-MP) and nanocellulose-chitosan-tripolyphosphate (NCT-MP) to enhance therapeutic efficacy. The encapsulation process was performed via ultrasonication and characterized by FTIR, SEM, and TEM, revealing polydisperse spherical particles with sizes of 566.37 ± 37.41 nm (NC-MP) and 756.66 ± 51.44 nm (NCT-MP). The anti-parkinsonian effects were evaluated in a catalepsy-induced mouse model using the horizontal bar test. Both encapsulated systems significantly reduced catalepsy duration (∗p∗ < 0.001). NC-MP demonstrated superior efficacy at low doses (5–20 mg/kg), attributed to its smaller particle size and neutral charge, which may facilitate blood-brain barrier penetration. In contrast, NCT-MP showed enhanced effects at 25 mg/kg, likely due to chitosan's cationic charge promoting active transport and tripolyphosphate-mediated matrix stabilization. These findings highlight the potential of encapsulation systems to optimize MP's therapeutic profile, with formulation efficacy dependent on dosage requirements.
{"title":"Encapsulation of Mucuna pruriens seed extract using nanocellulose and its evaluation for Parkinson's treatment","authors":"Ratnaningsih Eko Sardjono , Ramdhan Gunawan , Asep Kadarohman , Budiman Anwar , Hamidie Ronald Daniel Ray , Suci Nur Vikasari , Erdiwansyah , Siti Fatimah , Young Gun Ko","doi":"10.1016/j.jciso.2025.100144","DOIUrl":"10.1016/j.jciso.2025.100144","url":null,"abstract":"<div><div><em>Mucuna pruriens</em> (MP), a natural source of L-DOPA, holds potential as a Parkinson's disease (PD) treatment. This study developed two encapsulation systems for MP using nanocellulose (NC-MP) and nanocellulose-chitosan-tripolyphosphate (NCT-MP) to enhance therapeutic efficacy. The encapsulation process was performed via ultrasonication and characterized by FTIR, SEM, and TEM, revealing polydisperse spherical particles with sizes of 566.37 ± 37.41 nm (NC-MP) and 756.66 ± 51.44 nm (NCT-MP). The anti-parkinsonian effects were evaluated in a catalepsy-induced mouse model using the horizontal bar test. Both encapsulated systems significantly reduced catalepsy duration (∗p∗ < 0.001). NC-MP demonstrated superior efficacy at low doses (5–20 mg/kg), attributed to its smaller particle size and neutral charge, which may facilitate blood-brain barrier penetration. In contrast, NCT-MP showed enhanced effects at 25 mg/kg, likely due to chitosan's cationic charge promoting active transport and tripolyphosphate-mediated matrix stabilization. These findings highlight the potential of encapsulation systems to optimize MP's therapeutic profile, with formulation efficacy dependent on dosage requirements.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100144"},"PeriodicalIF":0.0,"publicationDate":"2025-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144704053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-07-17DOI: 10.1016/j.jciso.2025.100143
Solange Mailen Selzer , Laura Vanesa Bonnet , María Belén Piccoli , Santiago Daniel Salas , Mauricio Galiano , Raquel Viviana Vico , Nancy Fabiana Ferreyra
Iron oxide magnetic nanoparticles (IONPs) are widely utilized in biomedical and industrial applications due to their unique properties, including biocompatibility, superparamagnetism, and ease of functionalization. However, their behavior in biological environments is heavily influenced by surface functionalization and the formation of the protein corona. This study investigates the impact of aryl-functionalization of iron oxide nanoparticles with carboxylic and amine groups on colloidal stability, protein corona formation, and biological interactions. The IONPs were synthesized and characterized for their physicochemical properties, including size, zeta potential, magnetic properties, and dispersibility in different media. The interaction of the nanoparticles with dipalmitoylphosphatidylcholine monolayers, as a model membrane, was evaluated. Cytotoxicity and autophagy induction were assessed in Chinese hamster ovary (CHO-K1) and cervical cancer (HeLa) cells, respectively. The results demonstrate that surface functionalization significantly alters protein corona composition, which in turn modulates nanoparticle stability, cellular uptake, and biological responses. The aryl-functionalized nanoparticles exhibited reduced interactions with cell membranes compared to unfunctionalized counterparts, and also lower autophagy induction, emphasizing the importance of surface design in minimizing adverse effects.
{"title":"From surface design to cellular Response: Insights into aryl-functionalized iron oxide nanoparticles with and without protein corona","authors":"Solange Mailen Selzer , Laura Vanesa Bonnet , María Belén Piccoli , Santiago Daniel Salas , Mauricio Galiano , Raquel Viviana Vico , Nancy Fabiana Ferreyra","doi":"10.1016/j.jciso.2025.100143","DOIUrl":"10.1016/j.jciso.2025.100143","url":null,"abstract":"<div><div>Iron oxide magnetic nanoparticles (IONPs) are widely utilized in biomedical and industrial applications due to their unique properties, including biocompatibility, superparamagnetism, and ease of functionalization. However, their behavior in biological environments is heavily influenced by surface functionalization and the formation of the protein corona. This study investigates the impact of aryl-functionalization of iron oxide nanoparticles with carboxylic and amine groups on colloidal stability, protein corona formation, and biological interactions. The IONPs were synthesized and characterized for their physicochemical properties, including size, zeta potential, magnetic properties, and dispersibility in different media. The interaction of the nanoparticles with dipalmitoylphosphatidylcholine monolayers, as a model membrane, was evaluated. Cytotoxicity and autophagy induction were assessed in Chinese hamster ovary (CHO-K1) and cervical cancer (HeLa) cells, respectively. The results demonstrate that surface functionalization significantly alters protein corona composition, which in turn modulates nanoparticle stability, cellular uptake, and biological responses. The aryl-functionalized nanoparticles exhibited reduced interactions with cell membranes compared to unfunctionalized counterparts, and also lower autophagy induction, emphasizing the importance of surface design in minimizing adverse effects.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100143"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144672143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-30DOI: 10.1016/j.jciso.2025.100142
Ahmed F. Aman , Omayma A. Ghazy , Hoda Saleh , Ismaiel A. Ali , Neamat H. Ahmed , Sameh A. Rizk , Zakaria I. Ali
This study investigates the synthesis of magnetite/chitosan nanocomposites (Fe3O4@CS) using in-situ and ex-situ methods. The nanocomposites were characterized by FTIR, XRD, TEM, and VSM analyses. Their potential in nanomedicine was assessed through chemosensitivity tests on Ehrlich ascites carcinoma (EAC) and hyperthermic performance under an alternating magnetic field. The particle size of Fe3O4 nanoparticles was 10–20 nm and 20–40 nm for in-situ and ex-situ prepared samples as revealed by TEM images. FTIR indicated strong interactions between magnetite and chitosan, while XRD revealed a more uniform crystalline structure of Fe3O4 nanoparticles in ex-situ prepared samples. VSM analysis showed higher magnetization saturation for the ex-situ samples, with magnetization decreasing as chitosan content increased. In vitro assays demonstrated that both synthesis routes influenced the cytotoxicity of the nanocomposites against EAC cells, with in-situ samples showing greater early-stage cytotoxicity causing 40 % reduction in cell viability at 10 μg/mL. Under an alternating magnetic field, Fe3O4@CS nanocomposites reached therapeutic hyperthermia temperatures, with in-situ samples achieving 40.1–41.4 °C and ex-situ samples reaching 41–43 °C. These results highlight the importance of synthesis method and chitosan content in tailoring Fe3O4@CS nanocomposites for efficient and targeted hyperthermia-based cancer treatment.
{"title":"Enhanced biomedical performance of magnetic Fe3O4@chitosan nanocomposites: A comparative study of synthesis methods for hyperthermia and chemotherapy applications","authors":"Ahmed F. Aman , Omayma A. Ghazy , Hoda Saleh , Ismaiel A. Ali , Neamat H. Ahmed , Sameh A. Rizk , Zakaria I. Ali","doi":"10.1016/j.jciso.2025.100142","DOIUrl":"10.1016/j.jciso.2025.100142","url":null,"abstract":"<div><div>This study investigates the synthesis of magnetite/chitosan nanocomposites (Fe<sub>3</sub>O<sub>4</sub>@CS) using <em>in-situ</em> and <em>ex-situ</em> methods. The nanocomposites were characterized by FTIR, XRD, TEM, and VSM analyses. Their potential in nanomedicine was assessed through chemosensitivity tests on Ehrlich ascites carcinoma (EAC) and hyperthermic performance under an alternating magnetic field. The particle size of Fe<sub>3</sub>O<sub>4</sub> nanoparticles was 10–20 nm and 20–40 nm for <em>in-situ</em> and <em>ex-situ</em> prepared samples as revealed by TEM images. FTIR indicated strong interactions between magnetite and chitosan, while XRD revealed a more uniform crystalline structure of Fe<sub>3</sub>O<sub>4</sub> nanoparticles in <em>ex-situ</em> prepared samples. VSM analysis showed higher magnetization saturation for the <em>ex-situ</em> samples, with magnetization decreasing as chitosan content increased. <em>In vitro</em> assays demonstrated that both synthesis routes influenced the cytotoxicity of the nanocomposites against EAC cells, with <em>in-situ</em> samples showing greater early-stage cytotoxicity causing 40 % reduction in cell viability at 10 μg/mL. Under an alternating magnetic field, Fe<sub>3</sub>O<sub>4</sub>@CS nanocomposites reached therapeutic hyperthermia temperatures, with <em>in-situ</em> samples achieving 40.1–41.4 °C and <em>ex-situ</em> samples reaching 41–43 °C. These results highlight the importance of synthesis method and chitosan content in tailoring Fe<sub>3</sub>O<sub>4</sub>@CS nanocomposites for efficient and targeted hyperthermia-based cancer treatment.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100142"},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144231275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-27DOI: 10.1016/j.jciso.2025.100141
Van-Hoa Nguyen , Hoa-Hung Lam , Minh-Tam K. Nguyen , Thi-An-Sa Do , Hong-Phuong Phan , Trung Dang-Bao
Adapting for a green and safe protocol, this work developed a straightforward polyol method for synthesizing spherical silver nanoparticles (AgNPs), with a median size of 12.2 nm in glycerol at a low temperature (60 °C). The multipurpose nature of glycerol, acting as both a reductant and an immobilizer, was explored, demonstrating the long-term stability of AgNPs in the resulting colloidal solution. The stability of AgNPs in glycerol was also contrasted with their stability in water and other polyols. The multiple applications of AgNPs in glycerol included their use as antibacterial agents (against both E. coli and S. aureus strains) and as an eco-friendly catalyst. At room temperature, the selective hydrogenation of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) obeyed a pseudo first-order kinetic model, exhibiting a rate constant of 0.1064 min−1. The kinetic and thermodynamic results confirmed that the rate-determining step involved an electron-transfer process from adsorbed hydrogen to 4-nitrophenolate on the AgNPs surface. Utilizing glycerol as a non-toxic solvent, this colloidal AgNPs solution can be easily stored and directly used without any additional purification.
{"title":"A clear-cut synthesis of silver nanoparticles using glycerol as a multipurpose medium toward catalytic hydrogenation and antibacterial","authors":"Van-Hoa Nguyen , Hoa-Hung Lam , Minh-Tam K. Nguyen , Thi-An-Sa Do , Hong-Phuong Phan , Trung Dang-Bao","doi":"10.1016/j.jciso.2025.100141","DOIUrl":"10.1016/j.jciso.2025.100141","url":null,"abstract":"<div><div>Adapting for a green and safe protocol, this work developed a straightforward polyol method for synthesizing spherical silver nanoparticles (AgNPs), with a median size of 12.2 nm in glycerol at a low temperature (60 °C). The multipurpose nature of glycerol, acting as both a reductant and an immobilizer, was explored, demonstrating the long-term stability of AgNPs in the resulting colloidal solution. The stability of AgNPs in glycerol was also contrasted with their stability in water and other polyols. The multiple applications of AgNPs in glycerol included their use as antibacterial agents (against both <em>E. coli</em> and <em>S. aureus</em> strains) and as an eco-friendly catalyst. At room temperature, the selective hydrogenation of 4-nitrophenol (4-NP) to 4-aminophenol (4-AP) obeyed a pseudo first-order kinetic model, exhibiting a rate constant of 0.1064 min<sup>−1</sup>. The kinetic and thermodynamic results confirmed that the rate-determining step involved an electron-transfer process from adsorbed hydrogen to 4-nitrophenolate on the AgNPs surface. Utilizing glycerol as a non-toxic solvent, this colloidal AgNPs solution can be easily stored and directly used without any additional purification.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"19 ","pages":"Article 100141"},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144170380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2025-05-13DOI: 10.1016/j.jciso.2025.100138
Aimê Gomes da Mata Kanzaki , Tiago de Sousa Araújo Cassiano , João Valeriano , Fabio Luis de Oliveira Paula , Leonardo Luiz e Castro
The standard DLVO theory offers a limited description of ionic-surfacted magnetic colloids in near aggregation regimes. Correcting the electrical double layer term for ionic surfactants is not enough to successfully simulate the systems. The correction of the van der Waals energy divergence at short interparticle distances is fundamental for proper Monte Carlo sampling of nanoparticles’ configurations. We compare different short-range interaction models and show that a more detailed model leads to Monte Carlo simulations that better match theoretical expectations. Studying the energy scaling with the number of particles, we observe a slight deviation from energy extensivity across all models, small but still detectable via Akaike’s information criterion. The more detailed model predicts a strong effect of particle-size dispersity on the transition between overall attraction and repulsion. More precise modeling can significantly affect numerical predictions, in particular, the effect of particle-size dispersity on the spatial structure of colloids with high volume fraction. This emphasizes the importance of nailing down better models for describing complex colloidal dispersions.
{"title":"Colloidal structure, energy extensivity and Monte Carlo sampling properties of improved short-range interaction models for surfactant-coated magnetic nanoparticles","authors":"Aimê Gomes da Mata Kanzaki , Tiago de Sousa Araújo Cassiano , João Valeriano , Fabio Luis de Oliveira Paula , Leonardo Luiz e Castro","doi":"10.1016/j.jciso.2025.100138","DOIUrl":"10.1016/j.jciso.2025.100138","url":null,"abstract":"<div><div>The standard DLVO theory offers a limited description of ionic-surfacted magnetic colloids in near aggregation regimes. Correcting the electrical double layer term for ionic surfactants is not enough to successfully simulate the systems. The correction of the van der Waals energy divergence at short interparticle distances is fundamental for proper Monte Carlo sampling of nanoparticles’ configurations. We compare different short-range interaction models and show that a more detailed model leads to Monte Carlo simulations that better match theoretical expectations. Studying the energy scaling with the number of particles, we observe a slight deviation from energy extensivity across all models, small but still detectable via Akaike’s information criterion. The more detailed model predicts a strong effect of particle-size dispersity on the transition between overall attraction and repulsion. More precise modeling can significantly affect numerical predictions, in particular, the effect of particle-size dispersity on the spatial structure of colloids with high volume fraction. This emphasizes the importance of nailing down better models for describing complex colloidal dispersions.</div></div>","PeriodicalId":73541,"journal":{"name":"JCIS open","volume":"18 ","pages":"Article 100138"},"PeriodicalIF":0.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144098467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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