Abstract Terahertz (THz) imaging is a promising technology that can accurately detect breast tumors during breast-conserving surgery. Researchers have studied THz imaging and spectroscopy techniques to improve breast tumor detection for the past 20 years. This paper presents the recent development of the holographic THz imaging (HTI) method for identifying breast tumors. To evaluate the effectiveness of this new approach, we have developed a numerical system that includes realistic breast models and an imaging processing model. Through various experiments, we have successfully used the proposed holographic THz imaging method to identify breast tumors. Our results have shown that this method can reconstruct high-quality breast images and accurately detect small tumor inclusions, providing the correct size and location information. Based on these promising results, further investigation is warranted to explore the potential of this approach for breast tumor detection in a faster and more cost-effective manner.
{"title":"Holographic Terahertz Imaging for Breast Cancer Detection","authors":"Lulu Wang, Mohammad Al-Rawi","doi":"10.1115/1.4063599","DOIUrl":"https://doi.org/10.1115/1.4063599","url":null,"abstract":"Abstract Terahertz (THz) imaging is a promising technology that can accurately detect breast tumors during breast-conserving surgery. Researchers have studied THz imaging and spectroscopy techniques to improve breast tumor detection for the past 20 years. This paper presents the recent development of the holographic THz imaging (HTI) method for identifying breast tumors. To evaluate the effectiveness of this new approach, we have developed a numerical system that includes realistic breast models and an imaging processing model. Through various experiments, we have successfully used the proposed holographic THz imaging method to identify breast tumors. Our results have shown that this method can reconstruct high-quality breast images and accurately detect small tumor inclusions, providing the correct size and location information. Based on these promising results, further investigation is warranted to explore the potential of this approach for breast tumor detection in a faster and more cost-effective manner.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"34 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135666583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract The alleviation of test anxiety is beneficial for improving students' academic performance. This article provides a brief introduction to test anxiety and corresponding psychological interventions, followed by a study on the psychological intervention of test anxiety conducted on 200 college students from Yanching Institute of Technology. During the research process, college students were divided into four groups, each consisting of 50 students. After examination, there were no significant differences in gender and grade proportions among the groups. Psychological interventions were conducted through group counseling sessions. Group 1 received cognitive therapy, Group 2 participated in reading therapy, Group 3 combined cognitive therapy with reading therapy, and Group 4 did not include test anxiety in their counseling sessions. The test anxiety degrees of four groups were measured before and after psychological interventions. After conducting psychological interventions, subjective evaluations were obtained from students regarding their perception of counseling classes. The final results indicated that there was no significant difference in the level of test anxiety among the four groups of students before undergoing psychological intervention (P > 0.05). However, after the intervention, a significant reduction in test anxiety was observed for students in Groups 1, 2, and 3 (P < 0.05), while Group 4 showed no significant change (P > 0.05). Among those who experienced a significant change after the intervention, Group 3 using cognitive therapy combined with reading therapy exhibited the greatest decrease in test anxiety levels. Furthermore, following the psychological intervention, Group 3 provided the highest subjective evaluation of counseling classes.
{"title":"Relieving Test Anxiety in College Students Through Different Psychological Intervention Programs","authors":"Ying Du, Jingjing Lan","doi":"10.1115/1.4063597","DOIUrl":"https://doi.org/10.1115/1.4063597","url":null,"abstract":"Abstract The alleviation of test anxiety is beneficial for improving students' academic performance. This article provides a brief introduction to test anxiety and corresponding psychological interventions, followed by a study on the psychological intervention of test anxiety conducted on 200 college students from Yanching Institute of Technology. During the research process, college students were divided into four groups, each consisting of 50 students. After examination, there were no significant differences in gender and grade proportions among the groups. Psychological interventions were conducted through group counseling sessions. Group 1 received cognitive therapy, Group 2 participated in reading therapy, Group 3 combined cognitive therapy with reading therapy, and Group 4 did not include test anxiety in their counseling sessions. The test anxiety degrees of four groups were measured before and after psychological interventions. After conducting psychological interventions, subjective evaluations were obtained from students regarding their perception of counseling classes. The final results indicated that there was no significant difference in the level of test anxiety among the four groups of students before undergoing psychological intervention (P &gt; 0.05). However, after the intervention, a significant reduction in test anxiety was observed for students in Groups 1, 2, and 3 (P &lt; 0.05), while Group 4 showed no significant change (P &gt; 0.05). Among those who experienced a significant change after the intervention, Group 3 using cognitive therapy combined with reading therapy exhibited the greatest decrease in test anxiety levels. Furthermore, following the psychological intervention, Group 3 provided the highest subjective evaluation of counseling classes.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"67 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135666584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Sky Papendorp, Ammy Ovando, Saleh Gharaie, Bobak Mosadegh, David Guerra-Zubiaga, Seyedhamidreza Alaie, Turaj Ashuri, Amir Ali Amiri Moghadam
Abstract This paper reports on the development of a novel soft robotic system for remote ultrasound applications. Direct contact of the ultrasound probe with the patient's body represents a safety risk and therefore control of the probe's positioning and applied force is a crucial task. The proposed robot uses a passive control system that provides safe interaction between the robot and the patient by leveraging soft robotics technology. The soft robot's structure can be considered as a nonlinear spring which can be designed to exert a safe force within the robot's workspace to guarantee the safety of human–robot interaction. The literature suggests that effective ultrasound imaging of both the heart and abdomen requires six degrees-of-freedom. These degrees-of-freedom consist of three translational motions, which are achieved using a novel hybrid soft cable-driven parallel robot, and three wrist motions, which is based on a universal joint design. The experimental results show that the robot can achieve all these six degrees-of-freedom, and its blocking force can be engineered to generate a uniform force within the workspace.
{"title":"Toward Development of Novel Remote Ultrasound Robotic System Using Soft Robotics Technology","authors":"Sky Papendorp, Ammy Ovando, Saleh Gharaie, Bobak Mosadegh, David Guerra-Zubiaga, Seyedhamidreza Alaie, Turaj Ashuri, Amir Ali Amiri Moghadam","doi":"10.1115/1.4063469","DOIUrl":"https://doi.org/10.1115/1.4063469","url":null,"abstract":"Abstract This paper reports on the development of a novel soft robotic system for remote ultrasound applications. Direct contact of the ultrasound probe with the patient's body represents a safety risk and therefore control of the probe's positioning and applied force is a crucial task. The proposed robot uses a passive control system that provides safe interaction between the robot and the patient by leveraging soft robotics technology. The soft robot's structure can be considered as a nonlinear spring which can be designed to exert a safe force within the robot's workspace to guarantee the safety of human–robot interaction. The literature suggests that effective ultrasound imaging of both the heart and abdomen requires six degrees-of-freedom. These degrees-of-freedom consist of three translational motions, which are achieved using a novel hybrid soft cable-driven parallel robot, and three wrist motions, which is based on a universal joint design. The experimental results show that the robot can achieve all these six degrees-of-freedom, and its blocking force can be engineered to generate a uniform force within the workspace.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135667258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Polo is a popular sport in New Zealand, Australia, the United Kingdom, and many other countries. Polo is a vigorous sport involving players and horses moving at speed and can result in head injuries caused by falls. Helmets play a vital role in the safety and protection of Polo players. This study investigates different hemispheric bumper shapes of the helmet to improve the impact resistance performance using finite element analysis (FEA) and explicit dynamics analysis (EDA). The aerodynamic performance of the proposed helmet is investigated using computational fluid dynamics (CFD) to account for drag impact on the Polo player's speed. These are investigated for impact resistance and the drag coefficient under different speeds. The EDA results show that the new proposed bumper will absorb the impact and reduce the energy transferred to the inside foam at a relative impact speed of 6.2 m/s, as recommended by the U.S. Consumer Product Safety Commission (CPSC), with a maximum total deformation of 4.42 mm compared to 4.19 mm and 3.85 mm for impact speeds of 5.9 m/s by the European standard PAS015:2011 helmets for equestrian use and 5.42 m/s BS EN 1078 helmets for bicyclists' use, respectively. Additionally, under speeds ranging from 15 km/h to 65 km/h, the new helmet demonstrated a drag coefficient of 0.454, similar to that of the national team helmet, at 0.423.
马球运动在新西兰、澳大利亚、英国和其他许多国家都很流行。马球是一项激烈的运动，涉及运动员和马匹的快速移动，可能会导致头部受伤。头盔在马球运动员的安全和保护中起着至关重要的作用。采用有限元分析(FEA)和显式动力学分析(EDA)对不同形状的半球形安全帽保险杠进行了研究，以提高其抗冲击性能。利用计算流体动力学(CFD)研究了该头盔的空气动力学性能，以考虑阻力对马球运动员速度的影响。研究了不同速度下的抗冲击性能和阻力系数。EDA结果表明,新提出的保险杠将吸收的影响和减少能量转移到内部泡沫的相对影响速度6.2米/秒,推荐由美国消费者产品安全委员会(消费品安全委员会),4.42毫米的最大总变形而影响4.19毫米和3.85毫米5.9 m / s的速度由欧洲标准PAS015:2011头盔用于马术5.42 m / s BS EN 1078头盔骑自行车的使用,分别。此外，在15公里/小时至65公里/小时的速度范围内，新头盔的阻力系数为0.454，与国家队头盔的0.423相似。
{"title":"Explicit and Computational Fluid Dynamics Analysis of a Novel Polo Helmet Design: A Parametric Study","authors":"Mohammad Al-Rawi, Li Zheng, Russ Best","doi":"10.1115/1.4063650","DOIUrl":"https://doi.org/10.1115/1.4063650","url":null,"abstract":"Abstract Polo is a popular sport in New Zealand, Australia, the United Kingdom, and many other countries. Polo is a vigorous sport involving players and horses moving at speed and can result in head injuries caused by falls. Helmets play a vital role in the safety and protection of Polo players. This study investigates different hemispheric bumper shapes of the helmet to improve the impact resistance performance using finite element analysis (FEA) and explicit dynamics analysis (EDA). The aerodynamic performance of the proposed helmet is investigated using computational fluid dynamics (CFD) to account for drag impact on the Polo player's speed. These are investigated for impact resistance and the drag coefficient under different speeds. The EDA results show that the new proposed bumper will absorb the impact and reduce the energy transferred to the inside foam at a relative impact speed of 6.2 m/s, as recommended by the U.S. Consumer Product Safety Commission (CPSC), with a maximum total deformation of 4.42 mm compared to 4.19 mm and 3.85 mm for impact speeds of 5.9 m/s by the European standard PAS015:2011 helmets for equestrian use and 5.42 m/s BS EN 1078 helmets for bicyclists' use, respectively. Additionally, under speeds ranging from 15 km/h to 65 km/h, the new helmet demonstrated a drag coefficient of 0.454, similar to that of the national team helmet, at 0.423.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"2 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135667708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract With the population of people affected by lower limb disability and physical impairments continuing to grow, engineers in response have begun to develop exoskeletons designed to assist and rehabilitate those in need. While there have been great strides and advancements in the development of exoskeletons, many of them are still too cumbersome, heavy, and expensive for most people. The project described in this paper aims to design and manufacture a wearable robotic knee exoskeleton that helps solve some of the drawbacks that exoskeletons have today. The exoskeleton is designed with lightweight and durable three-dimensional (3D)-printed PETG, TPU, and PLA components combined with soft, flexible, and wearable materials to achieve improved human–robot interaction while providing support when bending and extending the knee joint. The three main assemblies designed in this project were a 3D-printed semirigid knee chain, a 3D-printed flexible shin brace, and a motor actuator assembly mounted on a carbon fiber back plate. The semirigid knee chain is actuated using a Bowden cable which allows the heavy motor to be relocated onto the user's back. solidworks topology optimization and finite element analysis (FEA) were used to reduce weight while keeping the overall strength of the chain and ensuring the safety factor of 2. The exoskeleton was observed to be able to withstand applied torques of up to 29 N·m during the walking functionality test. This exoskeleton is also designed to be integrated into a larger hip exoskeleton system.
{"title":"Design and Fabrication of a Lightweight and Wearable Semi-Rigid Robotic Knee Chain Exoskeleton","authors":"Diego Rivera, Mojtaba Sharifi","doi":"10.1115/1.4063466","DOIUrl":"https://doi.org/10.1115/1.4063466","url":null,"abstract":"Abstract With the population of people affected by lower limb disability and physical impairments continuing to grow, engineers in response have begun to develop exoskeletons designed to assist and rehabilitate those in need. While there have been great strides and advancements in the development of exoskeletons, many of them are still too cumbersome, heavy, and expensive for most people. The project described in this paper aims to design and manufacture a wearable robotic knee exoskeleton that helps solve some of the drawbacks that exoskeletons have today. The exoskeleton is designed with lightweight and durable three-dimensional (3D)-printed PETG, TPU, and PLA components combined with soft, flexible, and wearable materials to achieve improved human–robot interaction while providing support when bending and extending the knee joint. The three main assemblies designed in this project were a 3D-printed semirigid knee chain, a 3D-printed flexible shin brace, and a motor actuator assembly mounted on a carbon fiber back plate. The semirigid knee chain is actuated using a Bowden cable which allows the heavy motor to be relocated onto the user's back. solidworks topology optimization and finite element analysis (FEA) were used to reduce weight while keeping the overall strength of the chain and ensuring the safety factor of 2. The exoskeleton was observed to be able to withstand applied torques of up to 29 N·m during the walking functionality test. This exoskeleton is also designed to be integrated into a larger hip exoskeleton system.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"269 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044303","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Numerical modeling is a useful approach for answering research questions that are either difficult or inefficient to answer experimentally. The goal of this study was to develop a validated numerical model of a stereolithography (SLA) manufactured mesostructure to predict compression behavior. A three-dimensional (3D) model was created for an 80 × 80 × 20 mm compression block. ANSYS was used to simulate compression testing of this structure. The lower plate of the structure was fixed while a vertical displacement was prescribed on the upper plate. The base material was modeled using a linearly elastic, isotropic material derived from Young's modulus (E), Poisson's ratio, and the ultimate tensile strength. The base material was varied to represent three different SLA polymers from Formlabs (i.e., three different models of the same geometrical structure). The shape of the force–displacement curves and compression force values agreed well between the model and previously collected experimental data. However, this agreement was limited to stiffer materials and only up to 10 mm of compression. This may be where the structure shifts from elastic to plastic behavior and some lattice structures fracture. This simulation tool can be used in the future to predict and optimize the behavior of this lattice structure but only while operating elastically.
{"title":"Finite Element Simulation of Compressing an Additively Manufactured Mesostructure","authors":"Anne Schmitz","doi":"10.1115/1.4063462","DOIUrl":"https://doi.org/10.1115/1.4063462","url":null,"abstract":"Abstract Numerical modeling is a useful approach for answering research questions that are either difficult or inefficient to answer experimentally. The goal of this study was to develop a validated numerical model of a stereolithography (SLA) manufactured mesostructure to predict compression behavior. A three-dimensional (3D) model was created for an 80 × 80 × 20 mm compression block. ANSYS was used to simulate compression testing of this structure. The lower plate of the structure was fixed while a vertical displacement was prescribed on the upper plate. The base material was modeled using a linearly elastic, isotropic material derived from Young's modulus (E), Poisson's ratio, and the ultimate tensile strength. The base material was varied to represent three different SLA polymers from Formlabs (i.e., three different models of the same geometrical structure). The shape of the force–displacement curves and compression force values agreed well between the model and previously collected experimental data. However, this agreement was limited to stiffer materials and only up to 10 mm of compression. This may be where the structure shifts from elastic to plastic behavior and some lattice structures fracture. This simulation tool can be used in the future to predict and optimize the behavior of this lattice structure but only while operating elastically.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"51 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jesse Gerringer, Karthik Somasundaram, Frank A. Pintar
Abstract Severe cervical spine injuries are rare in an automobile crash, however, the recovery for an individual is difficult. With no suitable surrogate in the laboratory setting, the exact head-neck (HN) response to severe impact accelerations is unknown. Therefore, this study aimed to develop a nonhuman primate (NHP) HN model and validate it using a historic NHP kinematic dataset that tested noninjury, as well as injury-inducing impact accelerations. The geometry of the NHP HN model was constructed from a previously CT-scanned skeleton and idealized as a two-dimensional quadrilateral shell mesh. Inertial properties of the vertebra and skull were defined, as well as 1D beam elements representing the spinal ligaments and discs. The model was then driven at the T1 vertebra using a literature-derived 10G acceleration curve to simulate frontal impact. Output peak Head X-acceleration was measured at 19.8G, which fell within the average peak response of 18.8 ± 4.6 G. Capsular ligament and interspinous ligament strains were measured along the cervical spine and the relative magnitudes were consistent with areas of likely injury at more severe impact accelerations. Once tested at more severe impact accelerations, this NHP HN model will provide a suitable way to study potential human cervical spine dynamics during frontal impact.
{"title":"Development and Validation of Non-Human Primate Head-Neck Computational Model for Frontal Impact Injury Analysis","authors":"Jesse Gerringer, Karthik Somasundaram, Frank A. Pintar","doi":"10.1115/1.4063471","DOIUrl":"https://doi.org/10.1115/1.4063471","url":null,"abstract":"Abstract Severe cervical spine injuries are rare in an automobile crash, however, the recovery for an individual is difficult. With no suitable surrogate in the laboratory setting, the exact head-neck (HN) response to severe impact accelerations is unknown. Therefore, this study aimed to develop a nonhuman primate (NHP) HN model and validate it using a historic NHP kinematic dataset that tested noninjury, as well as injury-inducing impact accelerations. The geometry of the NHP HN model was constructed from a previously CT-scanned skeleton and idealized as a two-dimensional quadrilateral shell mesh. Inertial properties of the vertebra and skull were defined, as well as 1D beam elements representing the spinal ligaments and discs. The model was then driven at the T1 vertebra using a literature-derived 10G acceleration curve to simulate frontal impact. Output peak Head X-acceleration was measured at 19.8G, which fell within the average peak response of 18.8 ± 4.6 G. Capsular ligament and interspinous ligament strains were measured along the cervical spine and the relative magnitudes were consistent with areas of likely injury at more severe impact accelerations. Once tested at more severe impact accelerations, this NHP HN model will provide a suitable way to study potential human cervical spine dynamics during frontal impact.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"50 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Md Ashiqur Rahman, Nazmul Islam, Robert Freeman, Ali Ashraf
Abstract Biosensing technology has advanced significantly in recent years because of its wide applications in healthcare and biomolecule detection. However, fabricating a biosensor that will be rapid, sensitive, inexpensive, simple, and selective can be challenging. In this study, we fabricated a paper-based biosensor using conductive polymers (CPs) and graphene to detect Interleukin-6 (IL-6) in human serum. Cross-sectional scanning electron microscopy (SEM) was carried out and the images indicated that flat graphene nanoflake (GNF) sheets are connected to the fibrous structure of cellulose. We used 20 μL of human serum and 2 μL of IL-6 antigen concentration to measure the electrical impedance spectroscopy (EIS) using Autolab potentiostat (PGSTAT302N). A typical three-electrode method was preferred over the two-electrode method for better accuracy, and the sensor was connected through an adapter to the potentiostat. The paper-based biosensor detected in the range of 2 pg/mL to 20 μg/mL (the solution was prepared by serial dilution method), and the charge transfer resistance (Rct) increases linearly with the concentration (899 Ω–964 Ω). The limit of detection (LOD) of IL-6 detection was ∼16 pg/mL for our biosensor. Thus, using a portable, simple, sensitive, and inexpensive paper-based biosensor can be a great platform to detect early-stage cancer biomarkers.
{"title":"Graphene Conductive Polymer Paper-Based Biosensor for Interleukin-6 Detection in Human Serum","authors":"Md Ashiqur Rahman, Nazmul Islam, Robert Freeman, Ali Ashraf","doi":"10.1115/1.4063468","DOIUrl":"https://doi.org/10.1115/1.4063468","url":null,"abstract":"Abstract Biosensing technology has advanced significantly in recent years because of its wide applications in healthcare and biomolecule detection. However, fabricating a biosensor that will be rapid, sensitive, inexpensive, simple, and selective can be challenging. In this study, we fabricated a paper-based biosensor using conductive polymers (CPs) and graphene to detect Interleukin-6 (IL-6) in human serum. Cross-sectional scanning electron microscopy (SEM) was carried out and the images indicated that flat graphene nanoflake (GNF) sheets are connected to the fibrous structure of cellulose. We used 20 μL of human serum and 2 μL of IL-6 antigen concentration to measure the electrical impedance spectroscopy (EIS) using Autolab potentiostat (PGSTAT302N). A typical three-electrode method was preferred over the two-electrode method for better accuracy, and the sensor was connected through an adapter to the potentiostat. The paper-based biosensor detected in the range of 2 pg/mL to 20 μg/mL (the solution was prepared by serial dilution method), and the charge transfer resistance (Rct) increases linearly with the concentration (899 Ω–964 Ω). The limit of detection (LOD) of IL-6 detection was ∼16 pg/mL for our biosensor. Thus, using a portable, simple, sensitive, and inexpensive paper-based biosensor can be a great platform to detect early-stage cancer biomarkers.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"114 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044295","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Jose Colmenarez, Yingnan Zhai, Valentina Ochoa Mendoza, Pengfei Dong, Kenia Nunes, Donny Won Suh, Linxia Gu
Abstract The biomechanical properties of the sclera such as the stiffness, anisotropic behavior, and nonlinear stress–strain relationship have been extensively investigated for the pathogenesis study of ocular diseases. Even so, scarce mechanical investigations have been conducted on the damage in the sclera when subjected to large and repetitive deformations. Hence, the aim of this study is to quantify microstructural damage of the posterior and anterior sclera, through mechanical testing and model fitting. We performed uniaxial mechanical tests on scleral strips dissected from African green monkeys. Samples were subjected to strain-driven cycles of 5%, 10%, 15%, and 20% to evaluate the damage behavior commonly known as the Mullins effect. Experimental results showed qualitative changes in the stress–stretch curves when higher loading cycles were applied. A pseudo-elastic model accurately captured the curve trends across all tested samples, as indicated by a coefficient of determination above 0.96 and a subsequent finite element analysis (FEA) validation. Damage evolution and resultant permanent set demonstrated that considerable microstructural failure was attainable even at small strain levels and that the inherent plasticity had a similar contribution to stress-softening as the Mullins effect. Computed material and damage properties are expected to provide a broader understanding of the underlying mechanisms of ocular diseases and the development of more effective approaches for their treatment.
{"title":"Damage-Induced Softening of the Sclera: A Pseudo-Elastic Modeling Approach","authors":"Jose Colmenarez, Yingnan Zhai, Valentina Ochoa Mendoza, Pengfei Dong, Kenia Nunes, Donny Won Suh, Linxia Gu","doi":"10.1115/1.4063467","DOIUrl":"https://doi.org/10.1115/1.4063467","url":null,"abstract":"Abstract The biomechanical properties of the sclera such as the stiffness, anisotropic behavior, and nonlinear stress–strain relationship have been extensively investigated for the pathogenesis study of ocular diseases. Even so, scarce mechanical investigations have been conducted on the damage in the sclera when subjected to large and repetitive deformations. Hence, the aim of this study is to quantify microstructural damage of the posterior and anterior sclera, through mechanical testing and model fitting. We performed uniaxial mechanical tests on scleral strips dissected from African green monkeys. Samples were subjected to strain-driven cycles of 5%, 10%, 15%, and 20% to evaluate the damage behavior commonly known as the Mullins effect. Experimental results showed qualitative changes in the stress–stretch curves when higher loading cycles were applied. A pseudo-elastic model accurately captured the curve trends across all tested samples, as indicated by a coefficient of determination above 0.96 and a subsequent finite element analysis (FEA) validation. Damage evolution and resultant permanent set demonstrated that considerable microstructural failure was attainable even at small strain levels and that the inherent plasticity had a similar contribution to stress-softening as the Mullins effect. Computed material and damage properties are expected to provide a broader understanding of the underlying mechanisms of ocular diseases and the development of more effective approaches for their treatment.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"93 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract In this study, cell behavior in a microchannel was tracked for the application of dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was fabricated inside the microchannel by photolithographic techniques: a triangular electrode with a tip angle of 0.26 rad and a rectangular electrode. A periodic alternating current of square wave with a period of 1 μs was introduced between the electrodes to induce an asymmetric electric field perpendicular to the mainstream direction. The behavior of mouse myoblasts (C2C12: mouse myoblast cell line) was measured in vitro while the suspension was flowing. The relationship between cell shift motion near the electrode and cell shape on the two-dimensional projection plane was investigated. Experimental results showed that cell movement in the direction perpendicular to the mainstream increased with geometries away from the circle in the two-dimensional projection plane. This method can be applied to sort cells according to their degree of shape deviation from a sphere. The dielectrophoretic effect can be applied to sort cells not only by cell size but also by cell deformation.
{"title":"Dielectrophoretic Movement of Cell Passing Between Surface Electrodes in Flow Channel","authors":"Shigehiro Hashimoto, Ryuya Ono","doi":"10.1115/1.4063463","DOIUrl":"https://doi.org/10.1115/1.4063463","url":null,"abstract":"Abstract In this study, cell behavior in a microchannel was tracked for the application of dielectrophoresis to biological cell sorting. A pair of titanium surface electrodes was fabricated inside the microchannel by photolithographic techniques: a triangular electrode with a tip angle of 0.26 rad and a rectangular electrode. A periodic alternating current of square wave with a period of 1 μs was introduced between the electrodes to induce an asymmetric electric field perpendicular to the mainstream direction. The behavior of mouse myoblasts (C2C12: mouse myoblast cell line) was measured in vitro while the suspension was flowing. The relationship between cell shift motion near the electrode and cell shape on the two-dimensional projection plane was investigated. Experimental results showed that cell movement in the direction perpendicular to the mainstream increased with geometries away from the circle in the two-dimensional projection plane. This method can be applied to sort cells according to their degree of shape deviation from a sphere. The dielectrophoretic effect can be applied to sort cells not only by cell size but also by cell deformation.","PeriodicalId":73734,"journal":{"name":"Journal of engineering and science in medical diagnostics and therapy","volume":"87 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135044301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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