Background: Corn silk, a traditional Chinese herbal medicine in China, has been used to remedy nephritis, hepatitis, measles, hyperglycemia, tumor, etc. In this study, flavonoid monomers in corn silk were isolated and identified. This study could serve as a basis for determining the curative effects of these monomers on human diseases. Method: Corn silk flavonoids were extracted using 80% (v/v) ethanol and isolated by repeated polyamide column and Toyopearl HW-40 column chromatography. Corn silk flavonoids were identified by UV spectroscopy, liquid chromatography/mass spectroscopy, and nuclear magnetic resonance spectroscopy. Results: Two C-glycoside flavones, namely, ax-4″-OH-3′-methoxymaysin and a 3′-methoxymaysin isomer, were isolated from corn silk and identified. Conclusion: The two C-glycoside flavones were successfully isolated from corn silk and identified. This study is the first to report 3′-methoxymaysin in corn silk.
{"title":"TWO C-GLYCOSIDE FLAVONES FROM CORN (ZEA MAYS) SILK","authors":"Shuncheng Ren","doi":"10.21010/AJTCAM.V15I4.4","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.4","url":null,"abstract":"Background: Corn silk, a traditional Chinese herbal medicine in China, has been used to remedy nephritis, hepatitis, \u0000measles, hyperglycemia, tumor, etc. In this study, flavonoid monomers in corn silk were isolated and identified. This \u0000study could serve as a basis for determining the curative effects of these monomers on human diseases. \u0000Method: Corn silk flavonoids were extracted using 80% (v/v) ethanol and isolated by repeated polyamide column and \u0000Toyopearl HW-40 column chromatography. Corn silk flavonoids were identified by UV spectroscopy, liquid \u0000chromatography/mass spectroscopy, and nuclear magnetic resonance spectroscopy. \u0000Results: Two C-glycoside flavones, namely, ax-4″-OH-3′-methoxymaysin and a 3′-methoxymaysin isomer, were \u0000isolated from corn silk and identified. \u0000Conclusion: The two C-glycoside flavones were successfully isolated from corn silk and identified. This study is the \u0000first to report 3′-methoxymaysin in corn silk.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75855967","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Most countries pose their own indigenous knowledge systems. Native knowledge on indigenous medicine is one of the knowledge systems widely used in many countries in Asia and Africa. In some countries, 80% of the population depend on indigenous medicine for primary health care. Sri Lanka is an island enriched with a wellestablished indigenous medical system. Materials and Methods: The objectives of the study were to identify characteristics, usage patterns, and perceptions of communities concerning indigenous medicine. Beneficiaries of indigenous medicine were selected through purposive sampling for this study. The data were collected through a documentary survey and interviews. Results: The study identified that the indigenous medical system of the country is called ‘DeshiyaChikitsa,’ ‘Sinhala Vedakama,’ or ‘Hela Vedakama,’ which encompass ritual healing practices, astrology, religious observances, and spiritual powers. Different approaches of people including the use of domestic medicine, obtaining the assistance of astrology, and rituals were identified. Regardless of age, level of education, or employment, communities trust indigenous medicine as a primary or a secondary option on the health care system of the country. The survey identified the growing demand for the indigenous medicine. Conclusion: Around 60 to 70% of the rural population which is comprise 77.3% of total population relies on indigenous medicine. Domestic medicine, astrology and rituals associated with indigenous medicine plays a vital role in people’s health care process. Experts in indigenous medicine are scattered in the country.
{"title":"AN INVESTIGATION INTO UTILIZATION, BELIEFS AND PRACTICE OF INDIGENOUS MEDICINE IN SRI LANKA","authors":"G. R. Padmasiri","doi":"10.21010/AJTCAM.V15I4.1","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.1","url":null,"abstract":"Background: Most countries pose their own indigenous knowledge systems. Native knowledge on indigenous \u0000medicine is one of the knowledge systems widely used in many countries in Asia and Africa. In some countries, 80% of \u0000the population depend on indigenous medicine for primary health care. Sri Lanka is an island enriched with a wellestablished \u0000indigenous medical system. \u0000Materials and Methods: The objectives of the study were to identify characteristics, usage patterns, and perceptions of \u0000communities concerning indigenous medicine. Beneficiaries of indigenous medicine were selected through purposive \u0000sampling for this study. The data were collected through a documentary survey and interviews. \u0000Results: The study identified that the indigenous medical system of the country is called ‘DeshiyaChikitsa,’ ‘Sinhala \u0000Vedakama,’ or ‘Hela Vedakama,’ which encompass ritual healing practices, astrology, religious observances, and \u0000spiritual powers. Different approaches of people including the use of domestic medicine, obtaining the assistance of \u0000astrology, and rituals were identified. Regardless of age, level of education, or employment, communities trust \u0000indigenous medicine as a primary or a secondary option on the health care system of the country. The survey identified \u0000the growing demand for the indigenous medicine. \u0000Conclusion: Around 60 to 70% of the rural population which is comprise 77.3% of total population relies on \u0000indigenous medicine. Domestic medicine, astrology and rituals associated with indigenous medicine plays a vital role \u0000in people’s health care process. Experts in indigenous medicine are scattered in the country.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75789882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: The roots of Dianthus thunbergii SS Hooper are used traditionally in South Africa for the treatment of diabetes, wounds, colic, chest complaints and cancer. This study was aimed at investigating the potential anti-proliferative activities of the D. thunbergii in mammalian cancer cell lines. Materials and Methods: Aqueous and ethanol extracts of D. thunbergii were tested in vitro on two cancer cell lines: human hepato-cellular carcinoma (HepG2) cells and murine insulinoma (INS-1) cells using the 3-(4,5-Dimethylthiazol-2- yl) 2,5-diphenyltetrazolium bromide (MTT) and crystal violet cell viability assays, as well as live-cell fluorescence imaging microscopy. A tentative profiling of the aqueous extract was also carried out using liquid chromatography-mass spectrometry (LC-MS). Results: The aqueous extract (50-200μg/ml) exhibited significant (p
{"title":"ANTI-PROLIFERATIVE ACTIVITIES OF THE AQUEOUS ROOT EXTRACT OF DIANTHUS THUNBERGII SS HOOPER (CARYOPHYLLACEAE)","authors":"A. Akinrinde, M. Venter, T. Koekemoer, G. Bradley","doi":"10.21010/AJTCAM.V15I4.5","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.5","url":null,"abstract":"Background: The roots of Dianthus thunbergii SS Hooper are used traditionally in South Africa for the treatment of \u0000diabetes, wounds, colic, chest complaints and cancer. This study was aimed at investigating the potential anti-proliferative \u0000activities of the D. thunbergii in mammalian cancer cell lines. \u0000Materials and Methods: Aqueous and ethanol extracts of D. thunbergii were tested in vitro on two cancer cell lines: \u0000human hepato-cellular carcinoma (HepG2) cells and murine insulinoma (INS-1) cells using the 3-(4,5-Dimethylthiazol-2- \u0000yl) 2,5-diphenyltetrazolium bromide (MTT) and crystal violet cell viability assays, as well as live-cell fluorescence imaging \u0000microscopy. A tentative profiling of the aqueous extract was also carried out using liquid chromatography-mass \u0000spectrometry (LC-MS). \u0000Results: The aqueous extract (50-200μg/ml) exhibited significant (p","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89685236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-10-16DOI: 10.21010/AJTCAM.V15I4.10
Abraham Gómez-Rivera, M. González-Cortázar, Ammy Joana Gallegos-García, Armando Escobar-Ramos, G. Flores‐Franco, C. E. Lobato-García
Background: Salvia gesneriflora Lindley is employed in traditional Mexican medicine for the treatment of several diseases. Materials and Methods: The chromatographic profile of three extracts (SgH, SgD and SgM) of this plant allowed the identification of 11 components in SgH, the presence of rosmarinic (1), chlorogenic (2) and caffeic (3) acids and quercetin glucoside (4) in SgM and of ursolic acid (5) in SgD. The spasmolytic (electrically induced contractions of guinea-pig ileum), anti-inflammatory (edema in mouse ear) and antioxidant potential (DPPH, ABTS and FRAP assays) of the extracts were evaluated. Results: SgM showed the highest percentage of relaxation (80.67 ± 1.633%) with no significant difference (p
{"title":"SPASMOLYTIC, ANTI-INFLAMMATORY, AND ANTIOXIDANT ACTIVITY OF SALVIA GESNERIFLORA LINDLEY","authors":"Abraham Gómez-Rivera, M. González-Cortázar, Ammy Joana Gallegos-García, Armando Escobar-Ramos, G. Flores‐Franco, C. E. Lobato-García","doi":"10.21010/AJTCAM.V15I4.10","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.10","url":null,"abstract":"Background: Salvia gesneriflora Lindley is employed in traditional Mexican medicine for the treatment \u0000of several diseases. \u0000Materials and Methods: The chromatographic profile of three extracts (SgH, SgD and SgM) of this \u0000plant allowed the identification of 11 components in SgH, the presence of rosmarinic (1), chlorogenic (2) \u0000and caffeic (3) acids and quercetin glucoside (4) in SgM and of ursolic acid (5) in SgD. The spasmolytic \u0000(electrically induced contractions of guinea-pig ileum), anti-inflammatory (edema in mouse ear) and \u0000antioxidant potential (DPPH, ABTS and FRAP assays) of the extracts were evaluated. \u0000Results: SgM showed the highest percentage of relaxation (80.67 ± 1.633%) with no significant \u0000difference (p","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"30 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85047305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Background: Nowadays, there has been an increase in the number of studies focused on the search for bioactive compounds produced by hydrolytic reactions from natural sources, such as the Quinoa grain, which represents an interesting agro-alimentary source that can have a beneficial influence on health, specifically antihypertensive potential. For this reason, the aim of the present study was to evaluate the antihypertensive activity of the protein hydrolysates obtained of Quinoa, which results important at the time to consider the incorporation of such peptides in the design of functional foods. Materials and Methods: Quinoa (Chenopodium quinoa Willd.) seeds were ground and the obtained flour was degreased and the protein isolate was obtained by isoelectric precipitation. The protein isolate was enzymatically hydrolyzed with Alcalase® and Flavourzyme® and the antihypertensive effect of peptides against angiotensin converting enzyme was evaluate using a mixture of 50 μL of sample, 50 μL of ACE working solution, 200 μL of substrate working solution and the fluorescence was determined with a microplate fluorometer following these characteristics: λ (excitation) = 355-375 nm; λ (emission) = 400-430 nm. Results: Peptides obtained using Alcalase® (protein content= 72.13%; DH= 31.22%) showed the highest inhibitory activity against the angiotensin converting enzyme (ACE), close to 88%. Conclusion: The Quinoa protein hydrolysates can be considered as a new agri-food source to be incorporated in the elaboration of functional foods with antihypertensive potential.
{"title":"ANTIHYPERTENSIVE ACTIVITY OF QUINOA (Chenopodium quinoa Willd.) PROTEIN HYDROLYSATES","authors":"S. Moreno-Limón, R. González-Luna","doi":"10.21010/AJTCAM.V15I4.3","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.3","url":null,"abstract":"Background: Nowadays, there has been an increase in the number of studies focused on the search for bioactive \u0000compounds produced by hydrolytic reactions from natural sources, such as the Quinoa grain, which represents an \u0000interesting agro-alimentary source that can have a beneficial influence on health, specifically antihypertensive potential. For \u0000this reason, the aim of the present study was to evaluate the antihypertensive activity of the protein hydrolysates obtained of \u0000Quinoa, which results important at the time to consider the incorporation of such peptides in the design of functional foods. \u0000Materials and Methods: Quinoa (Chenopodium quinoa Willd.) seeds were ground and the obtained flour was degreased \u0000and the protein isolate was obtained by isoelectric precipitation. The protein isolate was enzymatically hydrolyzed with \u0000Alcalase® and Flavourzyme® and the antihypertensive effect of peptides against angiotensin converting enzyme was \u0000evaluate using a mixture of 50 μL of sample, 50 μL of ACE working solution, 200 μL of substrate working solution and the \u0000fluorescence was determined with a microplate fluorometer following these characteristics: λ (excitation) = 355-375 nm; λ \u0000(emission) = 400-430 nm. \u0000Results: Peptides obtained using Alcalase® (protein content= 72.13%; DH= 31.22%) showed the highest inhibitory \u0000activity against the angiotensin converting enzyme (ACE), close to 88%. \u0000Conclusion: The Quinoa protein hydrolysates can be considered as a new agri-food source to be incorporated in the \u0000elaboration of functional foods with antihypertensive potential.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"38 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90225387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
F. Nasr, N. Abutaha, M. Z. Alzahrani, Muhammad Farooq, M. Wadaan
Background: Medicinal plants have been used in traditional medicine for the treatment of numerous diseases worldwide. There is a dire need for new anticancer agents and plants used in traditional medicine are a particularly useful source. Materials and methods: In this study, extracts of five different plants that grow in the desert of Saudi Arabia were evaluated to assess their cytotoxicity against the MDA-MB-231 breast cancer cell line. Soxhlet extraction was carried out on the leaves and stems using different solvents. The cytotoxicity of these extracts against MDA-MB-231 breast cancer cells was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The apoptotic cellular morphological changes were observed using inverted and fluorescence microscopes. Results: Our results showed that two of the five different medicinal plants (Rumex vesicarius and Malva parviflora) exhibited strong anticancer activity against the breast cancer cells. Specifically, 2 of the 40 extracts (from the five studied plants) showed promising activity. The chloroform extract of the stem of R. vesicarius (RSV CHCL3) exhibited moderate anticancer activity with a half-maximal inhibitory concentration (IC50) of 230 µg/mL while that of the hexane extract of M. parviflora stems (MPS Hex) was 248 µg/mL. Loss of cell integrity, shrinkage of the cytoplasm, and cell detachment were observed in the extract-treated MDA-MB-231 cells. Conclusion: R. vesicarius and M. parviflora chloroform and n-hexane stem extracts showed significant cytotoxicity against MDA-MB-231 human breast carcinoma cells.
{"title":"ANTICANCER POTENTIAL OF PLANT EXTRACTS FROM RIYADH (SAUDI ARABIA) ON MDA-MB-231 BREAST CANCER CELLS","authors":"F. Nasr, N. Abutaha, M. Z. Alzahrani, Muhammad Farooq, M. Wadaan","doi":"10.21010/AJTCAM.V15I4.7","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.7","url":null,"abstract":"Background: Medicinal plants have been used in traditional medicine for the treatment of numerous diseases worldwide. There is a dire need for new anticancer agents and plants used in traditional medicine are a particularly useful source. \u0000Materials and methods: In this study, extracts of five different plants that grow in the desert of Saudi Arabia were evaluated to assess their cytotoxicity against the MDA-MB-231 breast cancer cell line. Soxhlet extraction was carried out on the leaves and stems using different solvents. The cytotoxicity of these extracts against MDA-MB-231 breast cancer cells was assessed using the 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) and lactate dehydrogenase (LDH) assays. The apoptotic cellular morphological changes were observed using inverted and fluorescence microscopes. \u0000Results: Our results showed that two of the five different medicinal plants (Rumex vesicarius and Malva parviflora) exhibited strong anticancer activity against the breast cancer cells. Specifically, 2 of the 40 extracts (from the five studied plants) showed promising activity. The chloroform extract of the stem of R. vesicarius (RSV CHCL3) exhibited moderate anticancer activity with a half-maximal inhibitory concentration (IC50) of 230 µg/mL while that of the hexane extract of M. parviflora stems (MPS Hex) was 248 µg/mL. Loss of cell integrity, shrinkage of the cytoplasm, and cell detachment were observed in the extract-treated MDA-MB-231 cells. \u0000Conclusion: R. vesicarius and M. parviflora chloroform and n-hexane stem extracts showed significant cytotoxicity against MDA-MB-231 human breast carcinoma cells.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74855513","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Munirah, S.H Ahmad, H.M Ghazali, T. Mahmud, B.R Ketaren, N. Shukor, N.E Tajidin
Background: Clinacanthus nutans, also known as ‘Sabah snake grass’ or ‘Belalai gajah’, is a herb well known locally for its medicinal values. The primary chemical constituents of the leaves are schaftoside, vitexin, isovitexin, orientin and isoorientin, and antiviral activity is shown by two glycoglycerolipids. Despite the importance of C. nutans, complete information with respect to commercial production and postharvest handling of the herb in the local herbal industry is still lacking. Thus, the objective of this study was to determine the optimum postharvest handling processes that could retain the phytochemicals quality of C. nutans. Materials and Methods: The flavonoid compounds of C. nutans were analysed by using ultra fast liquid chromatography (UFLC). Total phenolic content and antioxidant activity were determined using a spectrophotometer. Results: The total phenolic compounds and antioxidant activity in C. nutans were found to be higher in the young vegetative stage than in the mature vegetative stage. Flavonoid compounds (schaftoside, isovitexin, vitexin and orientin) were also found to be highest in the young vegetative plant compared to the mature vegetative plant. All of the assayed phytochemicals and flavonoid compounds levels were found to be highest in oven dried samples compared to the sun, air and solar dried samples. Conslusion: This study suggests that oven-drying young vegetative C. nutans plant material is the optimum method to retain postharvest quality.
{"title":"PLANT VEGETATIVE STAGES AND DRYING METHODS AFFECT THE FLAVONOID CONTENT OF CLINACANTHUS NUTANS EXTRACTS","authors":"M. Munirah, S.H Ahmad, H.M Ghazali, T. Mahmud, B.R Ketaren, N. Shukor, N.E Tajidin","doi":"10.21010/AJTCAM.V15I4.8","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.8","url":null,"abstract":"Background: Clinacanthus nutans, also known as ‘Sabah snake grass’ or ‘Belalai gajah’, is a herb well known locally for its medicinal values. The primary chemical constituents of the leaves are schaftoside, vitexin, isovitexin, orientin and isoorientin, and antiviral activity is shown by two glycoglycerolipids. Despite the importance of C. nutans, complete information with respect to commercial production and postharvest handling of the herb in the local herbal industry is still lacking. Thus, the objective of this study was to determine the optimum postharvest handling processes that could retain the phytochemicals quality of C. nutans. \u0000Materials and Methods: The flavonoid compounds of C. nutans were analysed by using ultra fast liquid chromatography (UFLC). Total phenolic content and antioxidant activity were determined using a spectrophotometer. \u0000Results: The total phenolic compounds and antioxidant activity in C. nutans were found to be higher in the young vegetative stage than in the mature vegetative stage. Flavonoid compounds (schaftoside, isovitexin, vitexin and orientin) were also found to be highest in the young vegetative plant compared to the mature vegetative plant. All of the assayed phytochemicals and flavonoid compounds levels were found to be highest in oven dried samples compared to the sun, air and solar dried samples. \u0000Conslusion: This study suggests that oven-drying young vegetative C. nutans plant material is the optimum method to retain postharvest quality.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"37 4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74981200","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
M. Soares, C. A. Caneschi, M. Chaves, M. Mota, P. Stroppa, W. Barbosa, N. Raposo
Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were evaluated. Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) 10231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy (SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts. Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis. Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis.
{"title":"IN VITRO ANTIFUNGAL ACTIVITY AND CYTOTOXICITY SCREENING OF DRY CRUDE EXTRACTS FROM BRAZILIAN AMAZONIA PLANTS","authors":"M. Soares, C. A. Caneschi, M. Chaves, M. Mota, P. Stroppa, W. Barbosa, N. Raposo","doi":"10.21010/AJTCAM.V15I4.2","DOIUrl":"https://doi.org/10.21010/AJTCAM.V15I4.2","url":null,"abstract":"Background: Antifungal multidrug resistance has been reported worldwide and has stimulated investigations of plant \u0000species for the treatment of candidiasis. In particular, in vitro antifungal activities and cytotoxicity of dry extracts from \u0000Ceasalpinia ferrea (tul.) Martius, Brosimum acutifolium Huber, and Salacia impressifolia (Miers) A.C. Smith were \u0000evaluated. \u0000Materials and Methods: Minimum inhibitory concentrations (MIC) and minimum fungicide (MFC) values were \u0000established according to the protocol M27-A2 of the Clinical and Laboratory Standards Institute (CLSI). Subsequent \u0000evaluations were performed using strains of Candida albicans from the American Type Culture Collection (ATCC) \u000010231, clinical isolated Candida albicans, Candida glabrata (CCT) 0728, Candida krusei (FTI) CCT 1517, and \u0000Candida guilliermondii (CCT) 1890. Morphological changes were evaluated using scanning electron microscopy \u0000(SEM), and cytotoxicity was evaluated in murine L929 fibroblast cells after treatment with plant extracts. \u0000Results: MIC values indicated antifungal potential of all three extracts against the main fungi that causes candidiasis. \u0000Conclusion: In particular, C. ferrea showed promising antimicrobial potential against all strains. Hence, future studies \u0000are warranted to investigate pharmacologically active compounds from this extract that could be used as prototypes for \u0000drug development and/or as a source of raw pharmaceutical materials for the treatment of candidiasis.","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79246358","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"OPTIMIZATION OF ALKALINE Α-AMYLASE PRODUCTION BY THERMOPHILIC BACILLUS SUBTILIS","authors":"Nuha Bakheet Al-Johani, M. Al-Seeni, Y. Ahmed","doi":"10.21010/ajtcam.v15i4.6","DOIUrl":"https://doi.org/10.21010/ajtcam.v15i4.6","url":null,"abstract":"<jats:p>Nill</jats:p>","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"96 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81173491","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"ANANAS SATIVA INCREASES LONGEVITY THROUGH OXIDATIVE STRESS RESISTANCE IN DROSOPHILA MELANOGASTER","authors":"Ajagun-Ogunleye M. O","doi":"10.21010/ajtcam.v15i3.9","DOIUrl":"https://doi.org/10.21010/ajtcam.v15i3.9","url":null,"abstract":"","PeriodicalId":7408,"journal":{"name":"African Journal of Traditional, Complementary and Alternative Medicines","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2018-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90314184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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