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Control of ginseng leaf black spot disease by endophytic fungi 内生真菌防治人参叶黑斑病的研究
Pub Date : 2021-07-31 DOI: 10.5897/ajb2018.16696
Zhou Chunyuan, Xu Shiquan, Piao Xiangmin, Yan Meixia, Cui Lili, Wang Yingping
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引用次数: 2
Changes in some behavioral, hematological and biochemical indices of air-breathing Clarias gariepinus [BUCHELL, 1822] exposed to pharmaceutical effluent 暴露于制药废水中的呼吸性Clarias gariepinus的一些行为、血液学和生化指标的变化[BUCHELL, 1822]
Pub Date : 2021-07-31 DOI: 10.5897/AJB2018.16673
M. Ogundiran, T. Ayandiran
This work aimed at evaluating the behavioral, biochemical and hematological effects of pharmaceutical effluent in laboratory population of Clarias gariepinus using a static renewal bioassay system. Fish specimens were collected and exposed to five (0.04, 0.06, 0.08, 0.10 and 0.12 mgL-1) sublethal concentrations of the effluent including a control experiment. Different dose dependent behavioral responses such as erratic swimming, gasp for breath, restlessness, and constant upward movement were observed in exposed fish. There was a steady decrease in the value of red blood cell (RBC), hemoglobin (Hb) and packed cell volume (PCV) as concentrations increase compared to the control stock. White blood cell count was found to be significantly (p<0.05) higher as the concentration of the test medium increases. Irregular level of lymphocytes and granulocytes across all concentrations was observed and levels of lymphocytes and granulocytes were significantly (p<0.05) increased in all effluent-treated fish samples during the exposure period. Different levels of total protein, glucose, cholesterol, etc., were obtained in the control and effluent-treated fish samples (p<0.05). The levels of aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase (serum enzymes) and lactate dehydrogenase were significantly (p<0.05) higher in the exposed C. gariepinus. The hematological and biochemical alterations in the effluent treated C. gariepinus, which were strongly indicative of cellular damages. This might be attributed to toxic effects of the phamarceutical effluent. Consequently, direct discharged of untreated or partially treated pharmaceutical effluent should be discouraged as this calls for public health concern.   Key words: Hematology, biochemical, pharmaceutical, Clarias gariepinus, effluent.
本工作旨在使用静态更新生物测定系统评估药物废水在实验室种群中的行为、生化和血液学影响。收集鱼类样本,并将其暴露于五种(0.04、0.06、0.08、0.10和0.12 mgL-1)亚致死浓度的流出物中,包括对照实验。在暴露的鱼类中观察到不同的剂量依赖性行为反应,如不稳定的游泳、喘息、不安和持续的向上运动。与对照组相比,随着浓度的增加,红细胞(RBC)、血红蛋白(Hb)和堆积细胞体积(PCV)的值稳步下降。随着试验培养基浓度的增加,白细胞计数显著增加(p<0.05)。在暴露期内,观察到所有浓度的淋巴细胞和粒细胞水平不规则,所有污水处理的鱼类样本中淋巴细胞和粒细胞核水平显著增加(p<0.05)。对照和出水处理的鱼样品中总蛋白、葡萄糖、胆固醇等水平不同(p<0.05)。暴露的加里epinus的天冬氨酸转氨酶、丙氨酸转氨酶、碱性磷酸酶(血清酶)和乳酸脱氢酶水平显著(p<0.01)。处理后的C.gariepinus废水中的血液学和生化变化强烈表明细胞损伤。这可能归因于制药废水的毒性作用。因此,应禁止直接排放未经处理或部分处理的制药废水,因为这需要公众健康关注。关键词:血液学,生物化学,制药,桔梗,出水。
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引用次数: 0
Effect of light quality on in vitro germination, seedling growth and photosynthetic pigments production in wheat (Triticum aestivum L.) 光品质对小麦离体萌发、幼苗生长及光合色素产生的影响
Pub Date : 2021-07-31 DOI: 10.5897/ajb2021.17329
Felix Blidar Cristian, Semiredana Chis Remalia, Rodica Bota Sanda, Serban Georgeta, Delia Stanasel Oana
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引用次数: 2
CRISPR/Cas genome editing: A frontier for transforming precision cassava breeding CRISPR/Cas基因组编辑:转化木薯精准育种的前沿
Pub Date : 2021-06-30 DOI: 10.5897/AJB2021.17344
Bicko Steve Juma, C. Mweu, M. Piero, W. Mbinda
Improved agricultural production of essential crops through advanced breeding is important for increasing access to nutritious food for the world's rapidly growing population, which is expected to reach 9.8 billion by 2050. Recent advancements in the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein9 (CRISPR/Cas9) genome editing process, which uses single-guide RNA for genome editing, have made it easy, stable and efficient tool for targeted gene mutations, knockout and knock-in/replacement to boost crop yield. The CRISPR/Cas method is constantly being improved, and its applications have greatly expanded. It can be used to modify the genome sequence of any organism, including plants like cassava, to achieve the desired trait. As a result, CRISPR/Cas is regarded as a game-changing technology in plant biology. Here, we discuss the principles of operation, implementations and future prospects of CRISPR/Cas9 for efficient processing of individual genes in cassava cultures. Recent work on cassava crop with regards to the use of CRISPR/Cas9 for the plant improvement was also addressed.   Key words:  Manihot esculenta, CRISPR/Cas9, genome editing, gRNA, protospacer adjacent motif (PAM).
通过先进育种提高基本作物的农业生产,对于增加世界快速增长人口获得营养食品的机会至关重要,预计到2050年,世界人口将达到98亿。集群规则间隔短回文重复序列/CRISPR相关蛋白9(CRISPR/Cas9)基因组编辑过程的最新进展,使用单引导RNA进行基因组编辑,使其成为靶向基因突变、敲除和敲除/替换的简单、稳定和有效工具,以提高作物产量。CRISPR/Cas方法正在不断改进,其应用也大大扩展。它可以用来修改任何生物体的基因组序列,包括木薯等植物,以实现所需的特性。因此,CRISPR/Cas被认为是植物生物学中一种改变游戏规则的技术。在这里,我们讨论了CRISPR/Cas9在木薯培养物中有效处理单个基因的操作原理、实施和未来前景。还讨论了最近关于木薯作物使用CRISPR/Cas9进行植物改良的工作。关键词:Manihot esculenta,CRISPR/Cas9,基因组编辑,gRNA,原间隔区相邻基序(PAM)。
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引用次数: 3
Imaging from anatomic to molecular and atomic resolution scales: A review 从解剖学到分子和原子分辨率的成像:综述
Pub Date : 2021-06-30 DOI: 10.5897/AJB2016.15696
P. Kamweru
Imaging may be referred to as the ‘eyes of science’ as it provides scientists with highly informative multi-dimensional and multi-parameter data usually invisible to the naked eye. As instrumentation technologies and genetic engineering advances, it’s possible in modern times to observe and image highly dynamic biochemistry processes. This paper reviews positron emission tomography (PET), magnetic resonance imaging (MRI), computed tomography (CT), ultrasound, visible light microscopy, bioluminescence (BLI) and fluorescence mediated tomography (FMT) imaging techniques, highlighting the principles behind the operation of each technique, their major strengths and drawbacks. With the enhancement of the existing techniques and evolution of new ones, the future possibility of refined view of systems invisible to naked human eye is promising. More so is when two or more techniques are combined in biological systems analysis.   Key words: Optical, imaging, microscopy, resolution, fluorescence.
成像可以被称为“科学之眼”,因为它为科学家提供了通常肉眼看不到的信息量大的多维和多参数数据。随着仪器技术和基因工程的进步,在现代有可能观察和成像高度动态的生物化学过程。本文综述了正电子发射断层扫描(PET)、磁共振成像(MRI)、计算机断层扫描(CT)、超声、可见光显微镜、生物发光(BLI)和荧光介导断层扫描(FMT)成像技术,重点介绍了每种技术的操作原理及其主要优缺点。随着现有技术的增强和新技术的发展,对肉眼看不见的系统进行精细视图的未来可能性是有希望的。当两种或两种以上的技术结合在生物系统分析中时更是如此。关键词:光学,成像,显微镜,分辨率,荧光。
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引用次数: 1
Synthesis, characterization and antibacterial activity of AgNPS mediated by Ocimum sanctum 茜草介导AgNPS的合成、表征及抗菌活性研究
Pub Date : 2021-05-31 DOI: 10.5897/ajb2020.17292
V. A., S. R.
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引用次数: 0
Precise assessment of angular leaf spot severity using the Leaf Doctor app for common beans 使用叶子医生应用程序对普通豆进行角斑严重程度的精确评估
Pub Date : 2021-05-31 DOI: 10.5897/ajb2021.17324
Panhoca de Almeida Caléo, Francesco Janini Bonfante Gabriel, Fausto de Carvalho Paulino Jean, Cristina de Deus Bianca, Rodrigues Alves Patrício Flávia, Augusto Morais Carbonell Sérgio, Fernando Chiorato Alisson, Lasry Benchimol-Reis Luciana
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引用次数: 1
Molecular characterization of bacteria strians isolated from JIKO: A herbal preparation consumed in some parts of Kaduna metropolis, Nigeria 从JIKO分离的细菌条纹的分子特征:尼日利亚卡杜纳大都市部分地区食用的草药制剂
Pub Date : 2021-04-30 DOI: 10.5897/AJB2020.17260
E. Kereakede, M. Abdulsalami, N. Egbe, V. Yilwa, E. E. Oaikhena, E. O. Oladapo, A. Danjuma, K. Onuh
Herbal products are considered as phytomedicines which are used as complementary medicine and without adequate monitoring they are leased straightaway into the market. Preparation, transportation and handling of these products can lead to high microbial contamination which can be harmful to human health. The aim of this research was to molecularly characterize some multiple resistance bacteria obtained from JIKO which is a herbal preparation consumed in some parts of Kaduna metropolis. The genomic DNA was extracted from each bacteria isolates, 16s rRNA genes were amplified and sequenced. The sequenced obtained from the amplified genes were analyzed using Basic Local Alignment Search Tool (BLAST) of the National Center for Biotechnology Institute database to compare the genes with the exist genes with ones in GENbank. The results obtained confirmed the identities of four bacteria isolates: Three Bacillus cereus and a Serratia species.  It was concluded that herbal products can contain some bacterial contaminants if not properly handled.   Key words: Herbal, phytomedicines, contaminants, genomic.
草药产品被视为植物药物,用作补充药物,在没有充分监测的情况下,它们被直接租赁到市场上。这些产品的制备、运输和处理可能会导致高度的微生物污染,对人类健康有害。本研究的目的是对从JIKO获得的一些多重耐药性细菌进行分子表征,JIKO是卡杜纳大都市一些地区食用的一种草药制剂。从每个菌株中提取基因组DNA,扩增16s rRNA基因并测序。使用国家生物技术中心研究所数据库的基本局部比对搜索工具(BLAST)分析从扩增的基因获得的测序,以将基因与存在的基因与GENbank中的基因进行比较。所获得的结果证实了四种细菌分离物的身份:三种蜡样芽孢杆菌和一种沙雷氏菌。结论是,如果处理不当,草药产品可能含有一些细菌污染物。关键词:草药,植物药,污染物,基因组。
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引用次数: 1
Investigation of the anti-bacterial properties of mangrove fern, Acrostichum aureum in the Niger Delta, Nigeria 尼日利亚尼日尔三角洲红树蕨的抗菌特性研究
Pub Date : 2021-04-30 DOI: 10.5897/AJB2021.17307
Aroloye O. Numbere, E. Maduike
Mangroves render several ecosystem services globally, one of which is pharmacological products. This study was done to verify whether Acrostichum aureum, a species of mangrove, common in the Niger Delta has anti-bacterial property. Several leaf samples were plucked from the trees at Eagle Island and placed in a cooler and sent to the lab for further analysis. The leaves were dried and ground into fine powder with manual grinding machine and 100 g of the powdered sample was measured and placed in 1000 ml of each of the extraction solvents (hot water and methanol).  Five different concentrations (32.25 mg/ml, 65.5 mg/ml, 125 mg/l, 250 mg/l and 500 mg/l) were made from each of the extracts using dimethyl sulfoxide (DMSO). Furthermore, bacterial species (Escherichia coli, Salmonella paratyphi, Staphylococcus aureus, and Pseudomonas aeruginosa) were isolated and a stock of each sample was made in agar slant and stored in the refrigerator at 4°C.  Mueller Hinton Sensitivity Agar (MHA) medium was prepared in agar and triplicate discs of each of the concentrations made from the two extracts were placed on the medium (MHA). The zone of inhibition in diameter in all plates was measured and analyzed statistically using R statistics. The result revealed that there is a significant difference in the growth of the microbes on the different concentrations of A. aureum (F4, 100 = 4.02, P = 0.01). A. aureum had higher effect on E. coli and S. aureus. Similarly, the higher the concentrations of the extracts the more effective it is in controlling the bacteria.  Finally, the study revealed that A. aureum has antibacterial properties that can be employed in drug production to treat common diseases prevalent in the region.   Key words: Antibiotics, bacteria, Niger Delta, mangrove, microbial species, medicinal products.
红树林在全球范围内提供多种生态系统服务,其中一种是药理产品。本研究旨在验证尼日尔三角洲常见的红树林Acrostichum aurum是否具有抗菌特性。从鹰岛的树上摘下几片叶子样本,放入冷却器中,送往实验室进行进一步分析。将叶子干燥并用手动研磨机研磨成细粉末,测量100g粉末状样品并将其置于1000ml每种提取溶剂(热水和甲醇)中。使用二甲基亚砜(DMSO)从每种提取物中制备五种不同浓度(32.25mg/ml、65.5mg/ml、125mg/l、250mg/l和500mg/l)。此外,分离细菌种类(大肠杆菌、副伤寒沙门氏菌、金黄色葡萄球菌和铜绿假单胞菌),并在琼脂斜面中制备每个样品的储备,并在4°C下储存在冰箱中。在琼脂中制备Mueller-Hinton敏感琼脂(MHA)培养基,并将由两种提取物制备的每种浓度的一式三份的圆盘放置在培养基(MHA)上。测量所有平板中直径的抑制区,并使用R统计进行统计学分析。结果表明,不同浓度的金黄色葡萄球菌对微生物的生长有显著差异(F4100=4.02,P=0.01)。类似地,提取物的浓度越高,它在控制细菌方面就越有效。最后,研究表明,金黄色葡萄球菌具有抗菌特性,可用于药物生产,以治疗该地区流行的常见疾病。关键词:抗生素,细菌,尼日尔三角洲,红树林,微生物种类,医药产品。
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引用次数: 4
Composition and balance of the analytical fractionation of desert date (Balanites aegyptiaca L.) seeds harvested in Senegal 塞内加尔收获的沙漠椰枣(Balanites aegyptiaca L.)种子分析分级的组成和平衡
Pub Date : 2021-04-30 DOI: 10.5897/AJB2021.17327
Djibril Diedhiou, M. Faye, C. Laure, Virginie Vendenbossche, G. Vilarem, O. Sock, L. Rigal
The desert date (Balanites aegyptiaca L.) can be one of the most common trees in Senegal. Thus, the objective is to carry out an analytical fractionation of these seeds to consider prospects for valuing the different constituents and a diagram of fractionation processes. The physical characterization of the seeds made it possible to know the fruit-seed and kernel-hull proportions. The chemical composition of the seeds shows that three main fractions represent 90% of its dry matter: The fibrous (68%), the lipid (14%) and the proteins (nearly 9%). The kernel is made up of 34.50% (80% of the lipid) and 28.75% of proteins (or 93% of the protein) while the hull is essentially fibrous, 90% contains 85% of the fibers.  The high proportion of unsaturated fatty acids oleic and linoleic (73%) associated with those of saturated fatty acids (palmitic and stearic) in high proportions (27%) and the high contents of sterols (2.11 g/kg), and in tocopherols (512.40 mg/kg), make desert date oil an excellent food oil. On the other hand, it has qualities that make it suitable for use in various non-food applications. Proteins are made up of three main amino acids: glutamic acid (20.35%), arginine (14.42%) and aspartic acid (11.29%). The amino acid composition is close to that of oil seeds whose cakes are widely used in animal or even human food. Given their essentially lignocellulosic composition, the hulls obtained from shelling can be used as fuel or be extruded and used for the manufacture of composite agro-materials.   Key words: Desert date, Balanites aegyptiaca, seeds, analytical fractionation, proteins, lipids.
沙漠椰枣(Balanites aegyptiaca L.)是塞内加尔最常见的树木之一。因此,目的是对这些种子进行分析分级,以考虑评估不同成分的前景和分级过程图。种子的物理特性使我们有可能知道果实种子和果壳的比例。种子的化学成分表明,三个主要部分占其干物质的90%:纤维(68%)、脂质(14%)和蛋白质(近9%)。内核由34.50%(80%的脂质)和28.75%的蛋白质(或93%的蛋白质)组成,而外壳基本上是纤维状的,90%含有85%的纤维。高比例的不饱和脂肪酸油酸和亚油酸(73%)与高比例的饱和脂肪酸(棕榈酸和硬脂酸)(27%)以及高含量的甾醇(2.11克/公斤)和生育酚(512.40毫克/公斤)相结合,使沙漠大枣油成为一种极好的食用油。另一方面,它具有适合用于各种非食品应用的特性。蛋白质由三种主要氨基酸组成:谷氨酸(20.35%)、精氨酸(14.42%)和天冬氨酸(11.29%)。其氨基酸组成与动物甚至人类食品中广泛使用的油籽接近。考虑到其基本上是木质纤维素的组成,从脱壳获得的外壳可以用作燃料或被挤出并用于制造复合农业材料。关键词:沙漠枣,埃及Balanites aegyptiaca,种子,分析分馏,蛋白质,脂质。
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引用次数: 0
期刊
African Journal of Biotechnology
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