microPublication biology最新文献

Actinobacteriophage Magritte was isolated from soil in Columbia, TN using Microbacterium foliorum as a host. Magritte is a singleton with a siphovirus morphology and a large genome of 133,228 bp encoding 250 predicted genes, including 26 tRNA genes.

The microbiota influences the Drosophila melanogaster dietary preference for yeast (DPY). We previously identified four transposon insertion mutants in Acetobacter fabarum that significantly influence fly DPY, and three of these insertions were in genes that are associated with thiamine metabolism. Here, we tested if thiamine influences fly DPY in monoassociated flies. We show that thiamine and other B vitamins influence fly DPY and that the different mutants have distinct DPY responses to thiamine supplementation. Together, these experiments identify specific nutritional effectors of D. melanogaster DPY.

The evolution of developmental rates may drive morphological change. Caenorhabditis inopinata develops nearly twice as slowly as Caenorhabditis elegans . clk-1 encodes a hydroxylase required for synthesizing ubiquinone, and mutant clk-1 slow growth phenotypes can be rescued by supplying animals with a ubiquinone precursor analogue, 2,4-dihydroxybenzoate. RNA-seq data showing low clk-1 expression raised the possibility that C. inopinata grows slowly because of reduced ubiquinone biosynthesis. C. inopinata did not reveal a clear reduction in the age of maturation when reared on 2,4-dihydroxybenzoate. Further scrutiny of RNA-seq results revealed multiple ubiquinone metabolism genes have low expression in C. inopinata . Divergent clk-1 expression alone may not be a major driver of the evolution of slow development in this species.

The C. elegans E3 ubiquitin ligase RPM-1 consists of 3,766 amino acids, with a RING finger domain at the C-terminus that functions to target the DLK-1 kinase for degradation for synapse development and axon termination. rpms-1 ( for rpm-1 short, aka F07B7.12 ) resides 35 kb away from rpm-1 on chromosome V, and is a near-perfect 12 kb duplication of rpm-1 , including the entire promoter region and coding sequences. RPMS-1 consists of 1,964 amino acids and is identical to the N-terminal half of RPM-1 , except the last 40 amino acids. Previous studies showed that transgenic overexpression of the duplicated region of rpm-1 (+) did not rescue synapse defects of rpm-1 loss of function mutants. Here, using CRISPR editing, we generated a double knockout of rpm-1 and rpms-1 . We find that axon and synapse defects in rpm-1rpms-1 double mutants resemble those in rpm-1 single mutants. Expression levels of endogenously tagged DLK-1 protein are increased to a comparable degree in rpm-1 and rpm-1rpms-1 mutants, compared to the control. These data, along with previous transgene expression analysis, support the idea that rpms-1 does not have a major role in RPM-1-mediated cellular processes.

Polyfluoro-alkyl substances (PFAS) are widely distributed environmental contaminants linked to human toxicity and developmental delays, especially low birthweight (LBW). In this study, Human Umbilical Vein Endothelial Cells (HUVECs) were exposed to the PFAS perfluorooctanesulfonic acid (PFOS). After 48-hours, their proliferation, and differential gene expression were assessed. A small, yet significant, reduction in proliferation was seen at 50 μg/mL and 75 μg/mL. RNA sequencing showed that estrogen response and notch signaling pathways were significantly altered. This study increases our understanding of how PFAS may interfere with endothelial cell (HUVECs) functions which may have larger effects on fetal growth, development, and birthweight.

Phages EarickHC, Figueroism, FinalFrontier, SBlackberry, Skylord, and Slay were isolated from soil samples collected around Southern California using the host Microbacterium foliorum . All six phages are lytic and have a siphoviral morphology. Genomes are 39,843 to 52,992 bp in length and contain 58 to 91 protein coding genes.

Gene model for the ortholog of Ecdysone-inducible gene L2 ( ImpL2 ) in the May 2017 (Princeton ASM75419v2/DsimGB2) Genome Assembly (GenBank Accession: GCA_000754195.3 ) of Drosophila simulans . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus Drosophila using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

Gene model for the ortholog of Myc ( Myc ) in the May 2011 (Agencourt dana_caf1/DanaCAF1) Genome Assembly (GenBank Accession: GCA_000005115.1 ) of Drosophila ananassae . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus Drosophila using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

Human p97/VCP is a vital AAA ATPase (ATPase associated with diverse cellular activity) that plays critical roles in protein homeostasis by regulating autophagy, endosomal trafficking, and the ubiquitin-proteasome system. Global proteomics analysis of p97/VCP inhibition with CB-5083 has been performed in HCT116 colon cells. Here, we examined the impact of CB-5083 treatment in another cancer model, the HL-60 acute myeloid leukemia cell line, employing subcellular fractionation combined with label-free proteomics to analyze changes in protein levels across cytoplasmic, nuclear, and insoluble membrane protein compartments. The results reveal distinct compartment-specific protein regulation, providing insight into p97/VCP's cellular mechanisms and its potential for targeted therapeutic applications.

Anthropogenic litter is one of the most important factors that influence recreation users and their activities because of its correlation to the river and environmental health. We monitored pollution levels on the Illinois river, near Tahlequah, OK for three months and surveyed the publics opinion on the issue. Our goal was to get this data to local and state management agencies to management practices to keep the scenic Illinois River clean. We found aluminum cans in the most abundant, while survey participants said cigarette butts were of highest observable abundance. A total of 170 people took the survey, and many of them want to see change on the river. Change may have to come from cooperation with commercial float operators.