microPublication biology最新文献

Gene model for the ortholog of Ribosomal protein S6 kinase ( S6k ) in the Dyak_CAF1 Genome Assembly (GenBank Accession: GCA_000005975.1) of Drosophila yakuba . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus Drosophila using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

Pseudomonas aeruginosa is an important pathogen associated with both chronic wounds and bloodstream infections. Virulence factors required for the establishment of acute and chronic infections differ substantially. Since bacteremia can be a severe outcome of wound colonization, we performed a comparative analysis of virulence between P. aeruginosa strains isolated from the bloodstream and chronic wounds. Our results show that, in general, P. aeruginosa strains isolated from bloodstream infections are more virulent than strains that colonize chronic wounds.

Macrophages are present in various forms throughout metazoans and play conserved roles in phagocytosis, immunity, and tissue homeostasis. In Drosophila melanogaster' s larval hematopoietic organ, the lymph gland, transient caspase-mediated activation of caspase-activated DNase triggers the DNA damage response (DDR), which is crucial for macrophage-type cell differentiation. Here, we report that other Drosophila species having different-sized mature lymph glands show effector caspase activity and DDR similar to those in Drosophila melanogaster , indicating that the developmental mechanism regulating phagocytic macrophage differentiation is conserved in different species of Drosophila .

The reversal of phototaxis has been observed in a wide range of animal species. However, environmental chemicals that can cause a quick reversal of phototaxis have rarely been reported. Here we identified hypochlorous acid (HClO) as an inducer of phototactic reversal in Vargula hilgendorfii , also known as sea fireflies. This species shows innate photophobic swimming behavior away from light sources and 0.1% HClO triggers rapid positive phototactic responses, that is reversible upon washout. Phototactic assays using monochromatic light irradiation revealed the highest photophobic responses at 460 nm, while the HClO-induced photophilic responses were observed widely, even in UV light.

We report the discovery and genome sequence of mycobacteriophage Eugenia, isolated from soil samples collected in Akron, OH. Eugenia is a double-stranded DNA virus with a genome size of 69,139 bp, featuring 104 predicted protein-encoding genes, with 32 of these genes assigned putative functions.

Efficient delivery of sensitive nucleic acid payloads, including mRNA, in Caenorhabditis elegans remains challenging, especially with traditional, labor-intensive transgenesis methods. We addressed these challenges using polymeric nanogels (NGs) as an advanced platform for mRNA delivery in C. elegans . These polymeric delivery vehicles can be engineered to suit desired applications owing to their chemical versatility, resulting from the ability to conjugate multiple functional groups onto the same backbone. Here, we validate the in vivo RNA delivery potential of redox-responsive NGs. The NGs showed up to 72.4 % RNA encapsulation and 6.61 % loading efficiencies and facilitated the controlled release of the mRNA payloads at intracellular concentrations of the reducing agent glutathione, where most of the RNA was released within 24 hours. As a proof of concept, we successfully delivered green fluorescent protein (GFP)-expressing mRNA using NGs in C. elegans for the first time. Physicochemical characterization revealed uniform NG size and charge, and fluorescence microscopy confirmed GFP expression in the gut after 24 hours of treatment. Our findings show NGs' potential as an mRNA delivery system in C. elegans .

Bacteriophage Evcara is a podovirus isolated on Microbacterium paraoxydans NRRL B-24275. Its genome is 16,285 bp in length and contains 22 predicted protein-coding genes. Evcara, has been assigned to cluster GI with Microbacterium foliorum phages PineapplePizza and Curie that share 10 homologues with the well-characterized Bacillus subtilis phage phi29.

Gene model for the ortholog of Downstream of raf1 ( Dsor1 ) in the May 2011 (Broad dper_caf1/DperCAF1) Genome Assembly (GenBank Accession: GCA_000005195.1 ) of Drosophila persimilis . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus Drosophila using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

Gene model for the ortholog of Insulin-like peptide 5 ( Ilp5 ) in the D. ananassae May 2011 (Agencourt dana_caf1/DanaCAF1) Genome Assembly (GenBank Accession: GCA_000005115.1 ) of Drosophila ananassae . This ortholog was characterized as part of a developing dataset to study the evolution of the Insulin/insulin-like growth factor signaling pathway (IIS) across the genus Drosophila using the Genomics Education Partnership gene annotation protocol for Course-based Undergraduate Research Experiences.

Tall fescue ( Festuca arundinacea ) is a widely adopted forage and turf grass. This is partly due to a fungal endophyte, Epichloë coenophiala, which confers both abiotic and biotic stress tolerance. Although PCR primers exist to test for endophyte presence, these were not designed to quantitatively analyze the amount of fungus in the plant. In this study, we test different primer sets for quantitative biomass analysis of tall fescue and E. coenophiala. We report standard curves, r-squared, and efficiency values for every primer set and identify those most suited for qPCR in this system.