microPublication biology最新文献

We isolated and annotated a novel bacteriophage, RedRaider, on Gordonia terrae 3612. Annotation revealed genome features typical of cluster CR3 phages with the putative lysin A function encoded in two gene products, a protease C39 domain and a glycosyl hydrolase domain, and lysin B encoded distally in the left arm of the genome.

　 Enterococcus faecium T-110 (EFT110), Bacillus subtilis TO-A (BSTOA), and Clostridium butyricum TO-A (CBTOA) are probiotic bacteria used as supplements to improve intestinal symptoms in humans, chickens, pigs, cattle, and fish. However, their effects on hosts remain unclear. Our previous study showed that EFT110, BSTOA, and CBTOA extend the lifespan of Caenorhabditis elegans . In this study, we investigated the effects of these three strains on fat accumulation in nematodes; and observed that each bacterium significantly reduced fat accumulation by regulating gene expression in lipid metabolic pathways.

Actinobacteriophage LilTerminator was isolated from soil collected from Jekyll Island, Georgia, using Microbacterium foliorum NRRL B-24224 as the host. The genome of this phage is 39,963 bp, circularly permuted, and contains 62 predicted genes, including one tRNA gene. Based on gene content similarity of at least 35% to actinobacteriophages, LilTerminator is grouped into subcluster EA5.

We report the isolation and genomic sequencing of nine Actinomycetota obtained from both cave biofilms and trogloxenic Ceuthophilus (cave crickets). Strains were isolated from samples via actinomycetota-selective media and sequenced using nanopore sequencing. Provisional taxonomic inference of strains was performed by whole genome phylogenetic analysis, revealing a broad range of genera, with most strains clustering with known genera with digital DNA-DNA hybridization values less than 70%, and one strain not clustering with known genera. An analysis of secondary metabolism reveals the quantity and diversity of secondary metabolism in cave Actinomycetota.

Bacteriophage HamCheese and Kihatsu are siphoviruses isolated from soil in Raleigh, NC and Clayton, NC, respectively, using Arthrobacter globiformis , B2979. HamCheese is assigned to actinobacteriophage cluster AS based on gene content similarity. Its 38454 base pair genome encodes 67 putative genes. Kihatsu is assigned to cluster FF phage, with a genome 43,237 base pairs in length encoding 67 putative genes and 2 tRNAs. Based on gene content, both pages are predicted to be temperate.

During endocardial infections, viridans group streptococci use proteins containing siglec-like binding regions to engage sialic acid-capped O- GalNAc glycans on platelet glycoprotein GPIbα. Much past work used isolated di-, tri-, or tetrasaccharide partial ligands to interrogate this sialoglycan binding. Here, we report the 1.9 Å resolution crystal structure of the Streptococcus gordonii strain M99 siglec-like binding region bound to an L-serine-linked sialyl T antigen (sTa) trisaccharide. The structure demonstrates how trisaccharide extensions are accommodated, with implications for binding larger sialoglycan ligands.

Timelapse images of human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CMs) provide rich information on cell structure and contractile function. The two-dimensional cardiac muscle bundle (2DMB) platform standardizes tissue geometry, enabling physiologic, uniaxial contractions on elastomeric substrates. However, larger sarcomere displacements in 2DMBs challenge existing tracking pipelines. We present adaptations to SarcGraph, an open-source Python package for sarcomere detection and tracking, enabling automated analysis of high-frame-rate 2DMB videos. Key modifications include frame-by-frame detection with automated segmentation, Gaussian Process Regression for denoising, and automatic contractile phase detection. We provide 130 example movies through Harvard Dataverse, enabling high-throughput analysis and advancing hiPSC-CM research.

As part of the Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Sciences program, we report the discovery and genome sequence of microbacteriophages Crisis, BarnCat, Pochacco, Smelly, and SpiderBri. Phages were isolated from soil samples in Riverside, CA and Viroqua, WI using the hosts Microbacterium foliorum and Microbacterium paraoxydans.

In Drosophila melanogaster , the herbicide atrazine is known to alter longevity, accelerate development time, and cause modifications in protein production and gene expression related to oxidative stress. A low protein diet can affect fecundity and increase lifespan in flies. The present study investigated if and how different concentrations of dietary yeast, the main protein source for lab-reared flies, affect the lifespan of D. melanogaster exposed to atrazine. Atrazine exposure and yeast concentration did not affect adult longevity; however, there was a strong sex effect in that males displayed greater survival than females.

Phage IsHungry was isolated on Arthrobacter globiformis B-2979 and has siphovirus morphology. Its genome consists of 40,628 base pairs, encoding 61 putative genes and 3 tRNAs. As is typical of the phages with similar gene content that area assigned to actinobacteriophage FF cluster, all but 4 genes are transcribed unidirectionally.