Abstract Since the advent of confocal microscopy, advances in tissue clearing have allowed researchers to push the limits of imaging depth and dive deeper into the precise 3D structures of biological specimens. As clearing methods are discovered, refined, and retired, it behooves the prudent microscopist to consider the pros and cons of various tissue clearing methods and systematically evaluate whether tissue clearing is beneficial to answer their research questions. Herein, I will briefly discuss the evolution of tissue clearing, pros and cons of more popular methods and techniques related to fluorescence microscopy, and key considerations for selecting a method most likely to yield experimental success.
{"title":"Tissue Clearing: An Overview of Methods, Considerations, and Applications","authors":"Caleb A Padgett","doi":"10.1093/mictod/qaad086","DOIUrl":"https://doi.org/10.1093/mictod/qaad086","url":null,"abstract":"Abstract Since the advent of confocal microscopy, advances in tissue clearing have allowed researchers to push the limits of imaging depth and dive deeper into the precise 3D structures of biological specimens. As clearing methods are discovered, refined, and retired, it behooves the prudent microscopist to consider the pros and cons of various tissue clearing methods and systematically evaluate whether tissue clearing is beneficial to answer their research questions. Herein, I will briefly discuss the evolution of tissue clearing, pros and cons of more popular methods and techniques related to fluorescence microscopy, and key considerations for selecting a method most likely to yield experimental success.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135715343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Index of Advertisers","authors":"","doi":"10.1093/mictod/qaad094","DOIUrl":"https://doi.org/10.1093/mictod/qaad094","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135786641","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Highlights from Microscopy and Microanalysis","authors":"","doi":"10.1093/mictod/qaad070","DOIUrl":"https://doi.org/10.1093/mictod/qaad070","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135297846","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract In general, crystal twinning in accessory zircon represents a rare, but in almost all granites and gneisses, encounterable phenomenon. Since only the crystal face {101} occurs as a twin plane, formation of crystal twins turns out to be relatively simple. Besides geniculate twins, which develop by rotation of the crystal structure about the line [101], parallel twins that are arranged parallel to the crystallographic main axis of zircon can also be observed by light and electron microscopy. While geniculate twins usually show a frequency within the region of a permille, the number of parallel twins can rise to several percent depending on the investigated rock. In the present contribution, a brief insight into the microscopic world of zircon twinning is provided.
{"title":"Microscopic Visualization of Crystal Twinning in Accessory Zircon","authors":"Robert Sturm","doi":"10.1093/mictod/qaad071","DOIUrl":"https://doi.org/10.1093/mictod/qaad071","url":null,"abstract":"Abstract In general, crystal twinning in accessory zircon represents a rare, but in almost all granites and gneisses, encounterable phenomenon. Since only the crystal face {101} occurs as a twin plane, formation of crystal twins turns out to be relatively simple. Besides geniculate twins, which develop by rotation of the crystal structure about the line [101], parallel twins that are arranged parallel to the crystallographic main axis of zircon can also be observed by light and electron microscopy. While geniculate twins usually show a frequency within the region of a permille, the number of parallel twins can rise to several percent depending on the investigated rock. In the present contribution, a brief insight into the microscopic world of zircon twinning is provided.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135298349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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