{"title":"Empowering Future Leaders in Science Through the Student Microscopy Community","authors":"Thomas S Marchese, R. J. Spurling, Andrés Márquez","doi":"10.1093/mictod/qaae053","DOIUrl":"https://doi.org/10.1093/mictod/qaae053","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141841128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
As technology advances, the field of microscopy offers unique opportunities for scientific exploration. However, the complex terminology used to describe these cutting-edge techniques often appears as a jumble of letters, akin to alphabet soup. Among the plethora of acronyms, some of the most prevalent are associated with super-resolution microscopy, including Stimulated Emission Depletion Microscopy (STED), Structured Illumination Microscopy (SIM), Spatially Modulated Illumination (SMI), and Single-Molecule Localization Microscopy (SMLM). These techniques push past the limitations of conventional light microscopy, achieving resolutions down to the tens of nanometers. This article aims to decode these common super-resolution techniques, fostering a deeper understanding of microscopy and igniting interest in their application.
{"title":"The Alphabet Soup of Microscopy: An Introduction to Advanced Imaging Techniques. Part I: Super-Resolution’s STED, SIM, SMI, and SMLM","authors":"Austin Worden","doi":"10.1093/mictod/qaae048","DOIUrl":"https://doi.org/10.1093/mictod/qaae048","url":null,"abstract":"\u0000 As technology advances, the field of microscopy offers unique opportunities for scientific exploration. However, the complex terminology used to describe these cutting-edge techniques often appears as a jumble of letters, akin to alphabet soup. Among the plethora of acronyms, some of the most prevalent are associated with super-resolution microscopy, including Stimulated Emission Depletion Microscopy (STED), Structured Illumination Microscopy (SIM), Spatially Modulated Illumination (SMI), and Single-Molecule Localization Microscopy (SMLM). These techniques push past the limitations of conventional light microscopy, achieving resolutions down to the tens of nanometers. This article aims to decode these common super-resolution techniques, fostering a deeper understanding of microscopy and igniting interest in their application.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141850456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Nematode worms are the most abundant multicellular organism on Earth. They thrive in every habitat known, and they are voracious feeders within the top 70 cm of soils. Their sturdy cuticle protects them from environmental factors and predators. Nematodes play a significant role in the decomposition of vertebrate remains in soil and serve as indicators of nutrients that enter soils during decomposition. Certain parasitic nematodes have been identified in fossil remains, but reports of fossil worms are rare. We demonstrate the abundant presence of opportunistic nematodes feeding within dinosaur bones from the Hell Creek formation, MT. The presence of visible worm ultrastructure indicates that they were alive when preserved within the dinosaur bones. Our findings are identical to worms characterized as “blood parasites” in a dinosaur bone from Brazil, demonstrating that there is sufficient soft tissue within dinosaur bone canals to sustain large populations of nematodes post-mortem.
{"title":"Light and Electron Microscopy Study of Opportunistic Free-Living Nematodes Scavenging and Thriving within Buried Dinosaur Bones","authors":"M. Armitage","doi":"10.1093/mictod/qaad110","DOIUrl":"https://doi.org/10.1093/mictod/qaad110","url":null,"abstract":"\u0000 Nematode worms are the most abundant multicellular organism on Earth. They thrive in every habitat known, and they are voracious feeders within the top 70 cm of soils. Their sturdy cuticle protects them from environmental factors and predators. Nematodes play a significant role in the decomposition of vertebrate remains in soil and serve as indicators of nutrients that enter soils during decomposition. Certain parasitic nematodes have been identified in fossil remains, but reports of fossil worms are rare. We demonstrate the abundant presence of opportunistic nematodes feeding within dinosaur bones from the Hell Creek formation, MT. The presence of visible worm ultrastructure indicates that they were alive when preserved within the dinosaur bones. Our findings are identical to worms characterized as “blood parasites” in a dinosaur bone from Brazil, demonstrating that there is sufficient soft tissue within dinosaur bone canals to sustain large populations of nematodes post-mortem.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140522665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Steven L Goodman, Emily K Benson, Noah A Flint, Louis E Dye, Melissa F Chimento, Edward Phillips, Jeffrey W Percival, Grahame J Kidd
Electron microscopy (EM) is essential to the biological and biomedical sciences and clinical diagnostic pathology. Electron microscopy specimen preparation is laborious and time-consuming, with transmission EM (TEM) chemical preparation typically requiring 1–3 days, while volume electron microscopy (vEM) takes 3–5 days of tedious manual reagent exchanges every few minutes or hours. This places a considerable burden on laboratory scientists with the ongoing demand for TEM, and the rapidly growing demand for vEM due to its potential to revolutionize structural biology, connectomics, and related fields. This burden is exacerbated by a shortage of trained electron microscopy scientists as current staff retire, and few enter the workforce. This report provides four case studies to illustrate how automated and faster specimen preparation workflows using mPrep™ Automated Specimen Processors (ASP-1000™ and ASP-2000™, Microscopy Innovations, LLC) free electron microscopy staff in academic and pre-clinical research labs and a clinical pathology laboratory.
{"title":"Creating Efficient Workflows for Electron Microscopy Laboratories with Automated Specimen Preparation","authors":"Steven L Goodman, Emily K Benson, Noah A Flint, Louis E Dye, Melissa F Chimento, Edward Phillips, Jeffrey W Percival, Grahame J Kidd","doi":"10.1093/mictod/qaad108","DOIUrl":"https://doi.org/10.1093/mictod/qaad108","url":null,"abstract":"\u0000 Electron microscopy (EM) is essential to the biological and biomedical sciences and clinical diagnostic pathology. Electron microscopy specimen preparation is laborious and time-consuming, with transmission EM (TEM) chemical preparation typically requiring 1–3 days, while volume electron microscopy (vEM) takes 3–5 days of tedious manual reagent exchanges every few minutes or hours. This places a considerable burden on laboratory scientists with the ongoing demand for TEM, and the rapidly growing demand for vEM due to its potential to revolutionize structural biology, connectomics, and related fields. This burden is exacerbated by a shortage of trained electron microscopy scientists as current staff retire, and few enter the workforce. This report provides four case studies to illustrate how automated and faster specimen preparation workflows using mPrep™ Automated Specimen Processors (ASP-1000™ and ASP-2000™, Microscopy Innovations, LLC) free electron microscopy staff in academic and pre-clinical research labs and a clinical pathology laboratory.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140527345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Highlights from Microscopy and Microanalysis","authors":"","doi":"10.1093/mictod/qaad100","DOIUrl":"https://doi.org/10.1093/mictod/qaad100","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140520057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"How a Fish Can “See” with its Skin","authors":"S. Carmichael","doi":"10.1093/mictod/qaad101","DOIUrl":"https://doi.org/10.1093/mictod/qaad101","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140526597","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Super-resolution microscopy is becoming more and more relevant and widespread for solving complex biological questions. However, many super-resolution techniques require high-level expertise and specific sample preparation protocols limiting the kind of fluorophore that can be used and consequently the applications. CrestOptics DeepSIM supports researchers deploying standard sample preparation protocols used for confocal microscopy while offering staggering resolution, the possibility of doing live cell imaging, and the ability to image deep into tissues. DeepSIM also allows staining with commonly used fluorescent markers, making it compatible with a large variety of samples and applications.
{"title":"Easy Access to Super-Resolution Applications with DeepSIM","authors":"Carlo Brighi, Luca Clario, Sara Romanzi","doi":"10.1093/mictod/qaad105","DOIUrl":"https://doi.org/10.1093/mictod/qaad105","url":null,"abstract":"\u0000 Super-resolution microscopy is becoming more and more relevant and widespread for solving complex biological questions. However, many super-resolution techniques require high-level expertise and specific sample preparation protocols limiting the kind of fluorophore that can be used and consequently the applications. CrestOptics DeepSIM supports researchers deploying standard sample preparation protocols used for confocal microscopy while offering staggering resolution, the possibility of doing live cell imaging, and the ability to image deep into tissues. DeepSIM also allows staining with commonly used fluorescent markers, making it compatible with a large variety of samples and applications.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140523337","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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