Zach E Russell, Sayward “Russell” Grindley, Kristin Bunker, Louis T Germinario
{"title":"A Remembrance of Dr. Phillip E. Russell: Professor, Mentor, Researcher, and Entrepreneur","authors":"Zach E Russell, Sayward “Russell” Grindley, Kristin Bunker, Louis T Germinario","doi":"10.1093/mictod/qaad061","DOIUrl":"https://doi.org/10.1093/mictod/qaad061","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"388 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135297847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.
{"title":"Instant Volume Microscopy of Organoids with SOLIS","authors":"Florian Ströhl","doi":"10.1093/mictod/qaad064","DOIUrl":"https://doi.org/10.1093/mictod/qaad064","url":null,"abstract":"Abstract Advancements in microscopy techniques have revolutionized our ability to explore the intricacies of biological systems, with engineered human heart tissue (EHT) being a particularly challenging target. In this article, we will provide an in-depth look at the award-winning SOLIS technique (scanned oblique light-sheet instant-volume sectioning), a new twist on multifocus fluorescence microscopy. Through its unique capabilities, SOLIS offers a new approach to organoid and tissue imaging, providing unprecedented insights into the cellular architecture of these complex artificial samples. By being able to record optically sectioned volumes during single-camera exposures, SOLIS demonstrates remarkable advantages over traditional multifocus microscopy, underscoring its potential to transform our understanding of developmental biology, disease mechanisms, and potential therapeutic interventions.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"307 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135298348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Data-Driven Science: How AI and Open Data will Revolutionize Scientific Discovery","authors":"Ryan Brinkman","doi":"10.1093/mictod/qaad066","DOIUrl":"https://doi.org/10.1093/mictod/qaad066","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135297850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"Why We Feel Sick When We Have the Flu","authors":"Stephen W Carmichael","doi":"10.1093/mictod/qaad059","DOIUrl":"https://doi.org/10.1093/mictod/qaad059","url":null,"abstract":"","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135297851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Abstract Digital imaging has made scientific microscopy much faster and facilitated a range of imaging techniques not previously available. Advances in microscope digital image detection systems over the last decade have played an integral part in establishing digital imaging as the method of choice for most types of microscopy. Current systems represent advancements in hardware and software components adapted for both established techniques and for a variety of novel methods using light to extract microscopic detail from biological samples. These advanced systems provide an unprecedented ability to capture the most detail possible from a variety of sample types and image applications. With this complexity, however, comes the need to have a basic understanding of how individual camera systems operate so the best solution for an imaging need can be adopted. In this article, I will describe some key differences between camera options and how to evaluate whether a particular type of system is best suited to a specific research requirement. Although I have focused on the key attributes of systems for biological microscopy, the information is broadly applicable to other types of microscopic samples.
{"title":"Choosing the Best Camera System for Your Biological Light Microscopy Needs: Part II","authors":"W Gray (Jay) Jerome","doi":"10.1093/mictod/qaad063","DOIUrl":"https://doi.org/10.1093/mictod/qaad063","url":null,"abstract":"Abstract Digital imaging has made scientific microscopy much faster and facilitated a range of imaging techniques not previously available. Advances in microscope digital image detection systems over the last decade have played an integral part in establishing digital imaging as the method of choice for most types of microscopy. Current systems represent advancements in hardware and software components adapted for both established techniques and for a variety of novel methods using light to extract microscopic detail from biological samples. These advanced systems provide an unprecedented ability to capture the most detail possible from a variety of sample types and image applications. With this complexity, however, comes the need to have a basic understanding of how individual camera systems operate so the best solution for an imaging need can be adopted. In this article, I will describe some key differences between camera options and how to evaluate whether a particular type of system is best suited to a specific research requirement. Although I have focused on the key attributes of systems for biological microscopy, the information is broadly applicable to other types of microscopic samples.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":"43 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135297848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The use of microscope technology has proved to be foundational in the global advancement of science and medicine. However, fewer women are entering science and microscopy as a career. Many factors have been suggested for this decades-old trend, yet intense mentoring of female students by successful women in academia and industry may be reversing the trend. Our team designed a STEM microscopy curriculum in a mobile laboratory format to test the idea that more female students might show an interest in STEM training if they have a chance to operate a microscope. The interest in dinosaur remains is very high among secondary students, thus we reasoned that choosing dinosaur tissue remains as a specimen might attract such students to hands-on labs. Here we describe our efforts to attract female students to microscopy-related STEM content by using dinosaur soft tissue from our dinosaur digs as specimens during our hands-on microscope labs. We conducted 33 such labs in six states across the US over a 16-month period. Female student participation was over 52%. We suggest that the specimen choice (particularly dinosaur cells, veins, and nerves) in microscopy education provides a powerful incentive to female students to consider a science as a career.
{"title":"When Specimen Choice Wags the Microscopy Education Dog","authors":"M. Armitage","doi":"10.1093/mictod/qaad038","DOIUrl":"https://doi.org/10.1093/mictod/qaad038","url":null,"abstract":"\u0000 The use of microscope technology has proved to be foundational in the global advancement of science and medicine. However, fewer women are entering science and microscopy as a career. Many factors have been suggested for this decades-old trend, yet intense mentoring of female students by successful women in academia and industry may be reversing the trend. Our team designed a STEM microscopy curriculum in a mobile laboratory format to test the idea that more female students might show an interest in STEM training if they have a chance to operate a microscope. The interest in dinosaur remains is very high among secondary students, thus we reasoned that choosing dinosaur tissue remains as a specimen might attract such students to hands-on labs. Here we describe our efforts to attract female students to microscopy-related STEM content by using dinosaur soft tissue from our dinosaur digs as specimens during our hands-on microscope labs. We conducted 33 such labs in six states across the US over a 16-month period. Female student participation was over 52%. We suggest that the specimen choice (particularly dinosaur cells, veins, and nerves) in microscopy education provides a powerful incentive to female students to consider a science as a career.","PeriodicalId":74194,"journal":{"name":"Microscopy today","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45644208","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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