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Morphology (Dordrecht, Netherlands)最新文献

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어휘부 연구와 단어형성론 词汇研究与词汇形成论
Pub Date : 2021-05-31 DOI: 10.51157/KMOR.2021.23.1.1
H. Jeong
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引用次数: 0
Inflectional predictability and prosodic morphology in Pitjantjatjara and Yankunytjatjara pitjantjathara和yankunytjathara的屈折可预测性和韵律形态
Pub Date : 2021-03-26 DOI: 10.1007/s11525-021-09380-y
Sasha Wilmoth, John Mansfield
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引用次数: 6
Is the English writing system phonographic or lexical/morphological? A new look at the spelling of stems 英语的书写系统是语音系统还是词汇/形态系统?词干拼写的新视角
Pub Date : 2021-03-17 DOI: 10.1007/s11525-021-09379-5
Kristian Berg, Mark Aronoff
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引用次数: 2
Pseudo-ABA patterns in pronominal morphology 代词形态中的伪aba模式
Pub Date : 2021-03-09 DOI: 10.1007/s11525-021-09377-7
Janelle Middleton
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引用次数: 5
Introduction to the special issue morphological spelling 介绍特刊形态拼写
Pub Date : 2021-03-09 DOI: 10.1007/s11525-021-09378-6
Kristian Berg, M. Aronoff
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引用次数: 0
Special Issue: Phonological and phonetic variation in spoken morphology 特刊:语音形态的音位和语音变化
Pub Date : 2021-02-24 DOI: 10.1007/s11525-021-09376-8
Ruben van de Vijver, F. Tomaschek
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引用次数: 1
Correction to: How sensitive are adults to the role of morphology in spelling? 更正:成人对词法在拼写中的作用有多敏感?
Pub Date : 2021-02-02 DOI: 10.1007/S11525-021-09375-9
R. Treiman, Sloane Wolter, Brett Kessler
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引用次数: 0
Paradigmatic structure in the tonal inflection of Amuzgo 《阿穆兹戈》调性变调中的聚合结构
Pub Date : 2021-01-26 DOI: 10.1007/s11525-021-09373-x
E. Palancar
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引用次数: 4
Autophagy as a life support marker of isolated hepatocytes 自噬作为分离肝细胞的生命支持标志物
Pub Date : 2021-01-15 DOI: 10.17816/1026-3543-2021-159-1-5-12
N. Bgatova, R. Dossymbekova, Julia S. Taskaeva, S. Miroshnichenko, R. Knyazev, A. Solovieva, K. Sharipov, Z. B. Tungushbaeva
AIM: The work aimed to reveal structural signs of autophagy in the cytoplasm of isolated hepatocytes in the dynamics of their cultivation. MATERIALS AND METHODS: The cultivated hepatocyte culture cell cycle was studied by flow cytofluorometry. The cells were cultured for 1, 24, and 48 hours. Morphometric analysis was performed using of the computer program Image J. The diameters of the nuclei and cytoplasm of hepatocytes, the volumes of nuclei and cytoplasm, and the nuclear-cytoplasmic ratio were determined. The concentration of intracellular organelles and autophagy was evaluated with magnification by 30000 times. RESULTS: The cell cycle arrest in the G0/G1 stage after 24 hours of hepatocyte cultivation and the preservation of their viability by hour 48 of the experiment without increase in the percentage of cells in the apoptosis stage were revealed. The decrease in the absolute count of cells was registered, as well as an increase in the nuclear-cytoplasmic ratio indicating a decrease in the proportion of hepatocyte cytoplasm in the course of cultivation. After 24 hours of cultivation, autophagosomes with fragments of cytoplasm, glycogen rosettes, and autolysosomes with partially degraded material were revealed in the cell cytoplasm. By hour 48 of the study, a significant decrease in the volume density of glycogen and mitochondria was noted, as well as an increase in basal autophagy in hepatocytes, with a prevalence of glycophagy and mitophagy. CONCLUSIONS: Autophagy maintains cellular homeostasis of isolated hepatocytes under standard culture conditions, as evidenced by a decrease in the volume density of glycogen and mitochondria, and an increase in basal autophagy in the hepatocyte cytoplasm. The findings indicate the contribution of autophagy to the survival of the primary culture of hepatocytes and can be used as an indicator of the adequacy of culturing conditions.
目的:本研究旨在揭示分离肝细胞培养过程中细胞质中自噬的结构特征。材料与方法:采用流式细胞荧光法研究肝细胞培养周期。细胞分别培养1、24、48小时。用计算机程序Image j进行形态计量学分析,测定肝细胞细胞核和细胞质的直径、细胞核和细胞质的体积以及核质比。放大30000倍观察细胞内细胞器和自噬的浓度。结果:肝细胞培养24h后,细胞周期停滞在G0/G1期,实验48h时肝细胞活力保持,凋亡期细胞比例未增加。细胞的绝对数量减少,核质比增加,表明在培养过程中肝细胞的细胞质比例减少。培养24小时后,细胞质中可见带有细胞质碎片的自噬体、糖原莲座和含有部分降解物质的自噬体。在研究的第48小时,糖原和线粒体的体积密度显著降低,肝细胞的基础自噬增加,糖吞噬和线粒体自噬普遍存在。结论:在标准培养条件下,自噬维持了分离肝细胞的细胞稳态,证明了糖原和线粒体的体积密度降低,肝细胞细胞质的基础自噬增加。研究结果表明自噬对原代培养肝细胞存活的贡献,并可作为培养条件是否充足的指标。
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引用次数: 0
Immunophenotypic characteristics of inducible NO synthase expression in dentate gyrus of mature rats in modeling depression and its pharmacological correction 成熟大鼠齿状回诱导NO合成酶表达模型抑郁的免疫表型特征及其药理纠正
Pub Date : 2021-01-15 DOI: 10.17816/1026-3543-2021-159-1-21-28
A. Smirnov, M. Ekova, I. Tyurenkov, E. Volotova
AIM: The work aimed to investigate inducible NO synthase (iNOS) expression in dentate gyrus in mature rats when modeling depression, as well as the establish the pharmacological correction possibility of detected changes with Phenibut and compounds under laboratory codes of RSPU-189, RSPU-135. MATERIALS AND METHODS: Depressive-like behavior in animals was modeled by combining stressful stimuli such as loud sound, pulsating bright light, and vibration simultaneous with constant restriction of mobility and fluctuations in temperature of environment for 7 days (daily for 30 minutes). Changes in level of iNOS expression in dentate gyrus were assessed by calculating relative area of immunoreactive material (IRM) and staining intensity in points from 0 to 3. RESULTS: Compared with the control group, rats with experimental depression showed an increase in expression of iNOS-IRM in cytoplasm of neuronal perikarya in granular layer of dentate gyrus, as well as an increase in relative area of iNOS-IRM in neuropil and nerve cells. The use of the compound RSPU-189 (salifen) demonstrated to a greater extent the corrective effect, since in the cytoplasm of neuronal perikarya in granular layer of dentate gyrus of rats, there was a decrease in the expression of iNOS-IRM, as well as a decrease in the relative area of iNOS-IRM in neuropil and nerve cells, which corresponded to values of these parameters in the control group of animals. CONCLUSIONS: An experimental modeling of depression in dentate gyrus of mature rats revealed an increase of iNOS-IRM expression, the decrease of which was noted in its pharmacological correction with the compound RSPU-189 (salifen), which may indicate the predominant neuroprotective effect of this compound on GABAergic neurotransmission mechanisms.
目的:研究成年大鼠抑郁模型时齿状回诱导NO合成酶(iNOS)的表达情况,并建立Phenibut及实验室编码RSPU-189、RSPU-135的化合物对iNOS表达变化的药理学纠正可能性。材料与方法:在持续7天(每天30分钟)的环境温度波动和持续限制活动的同时,结合高声、脉冲强光、振动等应激刺激,模拟动物的抑郁样行为。通过计算免疫反应物质的相对面积(IRM)和0 ~ 3点的染色强度来评估齿状回iNOS表达水平的变化。结果:与对照组相比,实验性抑郁大鼠齿状回颗粒层神经元核周细胞质中iNOS-IRM表达增加,神经元和神经细胞中iNOS-IRM相对面积增加。化合物RSPU-189 (salifen)的使用更大程度上显示了矫正效果,因为在大鼠齿状回颗粒层神经元核周细胞质中,iNOS-IRM的表达减少,神经细胞和神经细胞中iNOS-IRM的相对面积减少,这与对照组动物的这些参数值相对应。结论:成熟大鼠齿状回抑郁的实验模型显示,iNOS-IRM表达增加,而化合物RSPU-189(沙利芬)对其进行药理学校正后,iNOS-IRM表达降低,这可能表明该化合物对gaba能神经传递机制具有主要的神经保护作用。
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引用次数: 0
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Morphology (Dordrecht, Netherlands)
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