Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1089
T. Terasaki, T. Kameya, T. Nakajima, M. Tsumuraya, Y. Shimosato, K. Kato, H. Ichinose, T. Nagatsu, T. Hasegawa
Two distinct cell lines were obtained from a single heterotransplanted tumor which had originated from a primary focus of small cell carcinoma of the lung (SCCL). They were maintained separately from the beginning in culture media with and without fetal calf serum supplementation. Cells in the serum-free medium grew mostly floating in loose aggregates and showed poor cell cohesiveness, scanty cytoplasm and a few intracytoplasmic small dense-cored granules; all of these features are characteristics of oat cell type SCCL. On the other hand, cells in the serum-supplemented medium grew mostly floating in flatter and more closely associated clumps, were larger, and showed increased cell cohesiveness, occasional tubular structures, better developed organelles including dense-cored granules, and an increased number of cell attachments; these features are characteristics of intermediate cell type SCCL. The modal number of chromosomes differed from each other. Neuron-specific enolase (gamma enolase) and aromatic L-amino acid decarboxylase (ADC) activities in cell pellets were significantly higher in both lines than in control non-small cell lung cancer cell lines. The alpha/gamma type enolase ratio was lower, as was the ADC activity, in serum-free cultures than in serum-supplemented cultures. Interchange of the culture medium induced changes of the growth pattern and cell type from "oat cell type" to "intermediate cell type" and vice versa. The chromosomal number also partially changed. These findings suggest that cultured cells of SCCL alter their growth pattern and cell type depending on the culture conditions and that the selective growth of one cell type might then take place.
{"title":"Interconversion of biological characteristics of small cell lung cancer depending on culture conditions.","authors":"T. Terasaki, T. Kameya, T. Nakajima, M. Tsumuraya, Y. Shimosato, K. Kato, H. Ichinose, T. Nagatsu, T. Hasegawa","doi":"10.20772/CANCERSCI1959.75.12_1089","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1089","url":null,"abstract":"Two distinct cell lines were obtained from a single heterotransplanted tumor which had originated from a primary focus of small cell carcinoma of the lung (SCCL). They were maintained separately from the beginning in culture media with and without fetal calf serum supplementation. Cells in the serum-free medium grew mostly floating in loose aggregates and showed poor cell cohesiveness, scanty cytoplasm and a few intracytoplasmic small dense-cored granules; all of these features are characteristics of oat cell type SCCL. On the other hand, cells in the serum-supplemented medium grew mostly floating in flatter and more closely associated clumps, were larger, and showed increased cell cohesiveness, occasional tubular structures, better developed organelles including dense-cored granules, and an increased number of cell attachments; these features are characteristics of intermediate cell type SCCL. The modal number of chromosomes differed from each other. Neuron-specific enolase (gamma enolase) and aromatic L-amino acid decarboxylase (ADC) activities in cell pellets were significantly higher in both lines than in control non-small cell lung cancer cell lines. The alpha/gamma type enolase ratio was lower, as was the ADC activity, in serum-free cultures than in serum-supplemented cultures. Interchange of the culture medium induced changes of the growth pattern and cell type from \"oat cell type\" to \"intermediate cell type\" and vice versa. The chromosomal number also partially changed. These findings suggest that cultured cells of SCCL alter their growth pattern and cell type depending on the culture conditions and that the selective growth of one cell type might then take place.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"16 1","pages":"1089-99"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86355302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1053
H. Ohgaki, T. Kato, K. Morino, N. Matsukura, S. Sato, S. Takayama, T. Sugimura
The effect of sodium chloride on the promotion stage of gastric carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was studied in male inbred Wistar rats. Rats in group I were given MNNG at a concentration of 50 micrograms/ml in their drinking water for 12 weeks and then 1 ml of saturated NaCl solution intragastrically once a week until experimental week 65. Rats in group II were given MNNG for 12 weeks and then 1 ml of distilled water intragastrically once a week until week 65. Rats in group III were not treated for the first 12 weeks and were then given 1 ml of saturated NaCl solution intragastrically once a week until week 65. The incidence of adenomatous hyperplasias in the glandular stomach was significantly higher in group I than in group II, but the incidences of gastric adenocarcinomas and adenomas in groups I and II were not significantly different. No neoplastic or preneoplastic changes were observed in the stomach in group III.
{"title":"Study of the promoting effect of sodium chloride on gastric carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine in inbred Wistar rats.","authors":"H. Ohgaki, T. Kato, K. Morino, N. Matsukura, S. Sato, S. Takayama, T. Sugimura","doi":"10.20772/CANCERSCI1959.75.12_1053","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1053","url":null,"abstract":"The effect of sodium chloride on the promotion stage of gastric carcinogenesis by N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) was studied in male inbred Wistar rats. Rats in group I were given MNNG at a concentration of 50 micrograms/ml in their drinking water for 12 weeks and then 1 ml of saturated NaCl solution intragastrically once a week until experimental week 65. Rats in group II were given MNNG for 12 weeks and then 1 ml of distilled water intragastrically once a week until week 65. Rats in group III were not treated for the first 12 weeks and were then given 1 ml of saturated NaCl solution intragastrically once a week until week 65. The incidence of adenomatous hyperplasias in the glandular stomach was significantly higher in group I than in group II, but the incidences of gastric adenocarcinomas and adenomas in groups I and II were not significantly different. No neoplastic or preneoplastic changes were observed in the stomach in group III.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"524 1","pages":"1053-7"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73484993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
It was found that three synthetic anthracycline analogs lacking not only antitumor activity but also calcium-antagonizing action possessed an activity to potentiate vincristine cytotoxicity against vincristine-resistant P388 leukemia. ID-8279, one of these analogs, significantly reversed resistance to vincristine and daunorubicin by increasing their intracellular accumulation.
{"title":"Reversal of multidrug resistance by non-antitumor anthracycline analogs.","authors":"Makoto Inaba, Kyoko Nagashima, Yoshio Sakurai, Masaru Fukui, Yoshikazu Yanagi","doi":"10.20772/CANCERSCI1959.75.12_1049","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1049","url":null,"abstract":"It was found that three synthetic anthracycline analogs lacking not only antitumor activity but also calcium-antagonizing action possessed an activity to potentiate vincristine cytotoxicity against vincristine-resistant P388 leukemia. ID-8279, one of these analogs, significantly reversed resistance to vincristine and daunorubicin by increasing their intracellular accumulation.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"27 1","pages":"1049-52"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88602860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1076
T. Miyagi, T. Goto, S. Tsuiki
The subcellular distribution of sialidase in rat hepatoma induced by 3'-methyl-4-dimethylaminoazobenzene was studied by using sialyllactose as a substrate in the pH range of 4.0-7.0. As found in rat liver, the activity was recovered largely in the mitochondrial/lysosomal fraction with an optimal pH of 4.5 and in the cytosolic fraction with an optimal pH of 6.0, although hepatoma lysosomal (acidic) sialidase was also distributed in the microsomal fraction. The lysosomal and cytosolic sialidases of the hepatoma were indistinguishable from the corresponding enzymes of liver in chromatographic behavior, kinetics and substrate specificity. The levels of lysosomal and cytosolic sialidase activities in liver and hepatomas were then studied in the pellet and supernatant fractions, respectively, obtained by centrifuging the postnuclear supernatant at 105,000g for 1 hr. All the hepatomas tested, one primary and three transplanted, showed higher lysosomal sialidase and lower cytosolic sialidase activities as compared with liver. Quantitative changes similar to those seen in hepatomas were observed in regenerating liver after partial hepatectomy.
{"title":"Sialidase of rat hepatomas: qualitative and quantitative comparison with rat liver sialidase.","authors":"T. Miyagi, T. Goto, S. Tsuiki","doi":"10.20772/CANCERSCI1959.75.12_1076","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1076","url":null,"abstract":"The subcellular distribution of sialidase in rat hepatoma induced by 3'-methyl-4-dimethylaminoazobenzene was studied by using sialyllactose as a substrate in the pH range of 4.0-7.0. As found in rat liver, the activity was recovered largely in the mitochondrial/lysosomal fraction with an optimal pH of 4.5 and in the cytosolic fraction with an optimal pH of 6.0, although hepatoma lysosomal (acidic) sialidase was also distributed in the microsomal fraction. The lysosomal and cytosolic sialidases of the hepatoma were indistinguishable from the corresponding enzymes of liver in chromatographic behavior, kinetics and substrate specificity. The levels of lysosomal and cytosolic sialidase activities in liver and hepatomas were then studied in the pellet and supernatant fractions, respectively, obtained by centrifuging the postnuclear supernatant at 105,000g for 1 hr. All the hepatomas tested, one primary and three transplanted, showed higher lysosomal sialidase and lower cytosolic sialidase activities as compared with liver. Quantitative changes similar to those seen in hepatomas were observed in regenerating liver after partial hepatectomy.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"66 1","pages":"1076-82"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86791570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1070
S. Hino, T. Kawamichi, M. Funakoshi, M. Kanamura, T. Kitamura, T. Miyamoto
The incidence of anti-adult T-cell leukemia-associated antigens (ATLA) was surveyed in 134 patients under chronic hemodialysis in the Nagasaki area, as well as 4708 blood donors resident in the same area as controls: 23 patients (17%) and 201 donors (4.3%) were positive for anti-ATLA antibody. All seropositive patients were found to have had a positive history of blood transfusions. Seroconversions were confirmed in 9 cases, all of them after initiation of transfusions. In total, 216 units of blood were transfused in 7 seroconverted patients, and this is consistent with the estimated rate of anti-ATLA antibody-positive donor blood supplied by the Red Cross Nagasaki Blood Center (5%).
{"title":"Transfusion-mediated spread of the human T-cell leukemia virus in chronic hemodialysis patients in a heavily endemic area, Nagasaki.","authors":"S. Hino, T. Kawamichi, M. Funakoshi, M. Kanamura, T. Kitamura, T. Miyamoto","doi":"10.20772/CANCERSCI1959.75.12_1070","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1070","url":null,"abstract":"The incidence of anti-adult T-cell leukemia-associated antigens (ATLA) was surveyed in 134 patients under chronic hemodialysis in the Nagasaki area, as well as 4708 blood donors resident in the same area as controls: 23 patients (17%) and 201 donors (4.3%) were positive for anti-ATLA antibody. All seropositive patients were found to have had a positive history of blood transfusions. Seroconversions were confirmed in 9 cases, all of them after initiation of transfusions. In total, 216 units of blood were transfused in 7 seroconverted patients, and this is consistent with the estimated rate of anti-ATLA antibody-positive donor blood supplied by the Red Cross Nagasaki Blood Center (5%).","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"61 1","pages":"1070-5"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88528548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1062
Y. Yamane, K. Sakai, T. Umeda, N. Murata, S. Ishizeki, I. Ogihara, A. Takahashi, I. Iwasaki, G. Ide
The effect of cupric acetate on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in rats was investigated. The surviving rats in the group given DMN (25 ppm) in the drinking water alone were killed at 26 weeks and it was found that 12 of 16 rats had developed liver tumors. In the group given DMN and cupric acetate (sc injections of 2 mg of Cu/kg of body weight once a week for 26 weeks), 7 of 22 rats developed liver tumors. The incidence of liver tumors in rats given DMN and cupric acetate was thus only about 40% of that in rats given DMN alone. No tumor was observed in the group given saline or cupric acetate alone. The thymidine incorporation into the liver DNA of rats was measured at 2 and 4 weeks after the start of the carcinogenicity experiment. The thymidine incorporation into the liver DNA of rats given DMN was significantly suppressed by the administration of cupric acetate. The methylation of liver DNA in rats given a single dose of DMN was also significantly suppressed by sc injection of cupric acetate; the formation of both O6-methylguanine and 7-methylguanine was reduced. This result suggests that sc injection of cupric acetate may have a suppressive effect on the initiation of carcinogenesis in the liver.
{"title":"Suppressive effect of cupric acetate on DNA alkylation, DNA synthesis and tumorigenesis in the liver of dimethylnitrosamine-treated rats.","authors":"Y. Yamane, K. Sakai, T. Umeda, N. Murata, S. Ishizeki, I. Ogihara, A. Takahashi, I. Iwasaki, G. Ide","doi":"10.20772/CANCERSCI1959.75.12_1062","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1062","url":null,"abstract":"The effect of cupric acetate on dimethylnitrosamine (DMN)-induced hepatocarcinogenesis in rats was investigated. The surviving rats in the group given DMN (25 ppm) in the drinking water alone were killed at 26 weeks and it was found that 12 of 16 rats had developed liver tumors. In the group given DMN and cupric acetate (sc injections of 2 mg of Cu/kg of body weight once a week for 26 weeks), 7 of 22 rats developed liver tumors. The incidence of liver tumors in rats given DMN and cupric acetate was thus only about 40% of that in rats given DMN alone. No tumor was observed in the group given saline or cupric acetate alone. The thymidine incorporation into the liver DNA of rats was measured at 2 and 4 weeks after the start of the carcinogenicity experiment. The thymidine incorporation into the liver DNA of rats given DMN was significantly suppressed by the administration of cupric acetate. The methylation of liver DNA in rats given a single dose of DMN was also significantly suppressed by sc injection of cupric acetate; the formation of both O6-methylguanine and 7-methylguanine was reduced. This result suggests that sc injection of cupric acetate may have a suppressive effect on the initiation of carcinogenesis in the liver.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"92 1","pages":"1062-9"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86972900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1108
Y. Koide, T. Hongo, R. Iseki, Y. Mori, T. Yoshida
T cells from 19 out of 25 childhood cancer patients showed impaired proliferative responses to purified protein derivatives (PPD)-pulsed antigen-presenting cells (APC) although all of the patients had been immunized with BCG. To test whether such low responsiveness of T cells results from the dysfunction of T cells or from that of APC, the experiment was designed to assess the proliferative response of T cells from patients or their parents to PPD-pulsed APC from patients or parents. These combinations seem to be suitable to assess the activity of T cells or APC since at least partial identity of HLA-D/DR antigens is required for T cell-APC interactions. Although T cells from patients who showed low responsiveness to PPD failed to respond even to PPD-pulsed APC from parents, T cells from parents were able to respond to PPD-pulsed APC from patients as well as to autologous APC. These observations strongly suggest that the low responsiveness to PPD in childhood cancer patients results from the dysfunction of T cells, and the capacity of APC is fully preserved. In other words, it appears that the capacity of APC is not impaired by chemotherapy, neoplastic cells, or other factors. Suppressor T cells appeared not to be involved in such dysfunction of T cells.
{"title":"The capacity of antigen-presenting cells is fully preserved in childhood cancer patients.","authors":"Y. Koide, T. Hongo, R. Iseki, Y. Mori, T. Yoshida","doi":"10.20772/CANCERSCI1959.75.12_1108","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1108","url":null,"abstract":"T cells from 19 out of 25 childhood cancer patients showed impaired proliferative responses to purified protein derivatives (PPD)-pulsed antigen-presenting cells (APC) although all of the patients had been immunized with BCG. To test whether such low responsiveness of T cells results from the dysfunction of T cells or from that of APC, the experiment was designed to assess the proliferative response of T cells from patients or their parents to PPD-pulsed APC from patients or parents. These combinations seem to be suitable to assess the activity of T cells or APC since at least partial identity of HLA-D/DR antigens is required for T cell-APC interactions. Although T cells from patients who showed low responsiveness to PPD failed to respond even to PPD-pulsed APC from parents, T cells from parents were able to respond to PPD-pulsed APC from patients as well as to autologous APC. These observations strongly suggest that the low responsiveness to PPD in childhood cancer patients results from the dysfunction of T cells, and the capacity of APC is fully preserved. In other words, it appears that the capacity of APC is not impaired by chemotherapy, neoplastic cells, or other factors. Suppressor T cells appeared not to be involved in such dysfunction of T cells.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"18 1","pages":"1108-15"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83800448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-12-01DOI: 10.20772/CANCERSCI1959.75.12_1058
S. Hosaka, H. Nagayama, I. Hirono
The effect of caffeine on the development of hepatic tumors induced by 2-acetylaminofluorene (2-AAF) was studied in 6-week-old male ACI rats. Rats in group 1 were fed a diet containing 0.02% 2-AAF for 18 weeks and then basal diet for 15 weeks with normal drinking water throughout. Animals in group 2 received a diet containing 0.02% 2-AAF and a solution of 0.2% caffeine as their drinking water for 18 weeks, followed by basal diet and caffeine-free water. Rats in group 3 received drinking water containing 0.2% caffeine for 18 weeks. Rats in group 4 were given a basal diet and water freely and served as controls. The experiment was terminated after 33 weeks. Both the multiplicity, i.e. the number of tumors per rat, and the size of tumors were less (P less than 0.001 in the former case, by Student's t-test) in group 2 than in group 1. Thus, the induction of tumors of the liver by 2-AAF was suppressed by the administration of caffeine.
{"title":"Suppressive effect of caffeine on the development of hepatic tumors induced by 2-acetylaminofluorene in ACI rats.","authors":"S. Hosaka, H. Nagayama, I. Hirono","doi":"10.20772/CANCERSCI1959.75.12_1058","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.12_1058","url":null,"abstract":"The effect of caffeine on the development of hepatic tumors induced by 2-acetylaminofluorene (2-AAF) was studied in 6-week-old male ACI rats. Rats in group 1 were fed a diet containing 0.02% 2-AAF for 18 weeks and then basal diet for 15 weeks with normal drinking water throughout. Animals in group 2 received a diet containing 0.02% 2-AAF and a solution of 0.2% caffeine as their drinking water for 18 weeks, followed by basal diet and caffeine-free water. Rats in group 3 received drinking water containing 0.2% caffeine for 18 weeks. Rats in group 4 were given a basal diet and water freely and served as controls. The experiment was terminated after 33 weeks. Both the multiplicity, i.e. the number of tumors per rat, and the size of tumors were less (P less than 0.001 in the former case, by Student's t-test) in group 2 than in group 1. Thus, the induction of tumors of the liver by 2-AAF was suppressed by the administration of caffeine.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"11 1","pages":"1058-61"},"PeriodicalIF":0.0,"publicationDate":"1984-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84652384","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-11-01DOI: 10.20772/CANCERSCI1959.75.11_1002
M. Bessho, I. Jinnai, K. Hirashima, K. Tanaka, H. Tanooka
The clonal origin of radiation-induced thymic lymphoma was studied in mice with cellular mosaicism for phosphoglycerate kinase (PGK). Repeated whole-body X-irradiations (4 doses, 1.7 Gy each) with intervals of 7 days resulted in development of thymic lymphomas in the mosaic mice. PGK from all lymphomas gave only a single spot on electrophoresis. The results demonstrate the single cell origin of the thymic lymphoma.
{"title":"Single cell origin of radiation-induced thymic lymphoma in mice with cellular mosaicism.","authors":"M. Bessho, I. Jinnai, K. Hirashima, K. Tanaka, H. Tanooka","doi":"10.20772/CANCERSCI1959.75.11_1002","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_1002","url":null,"abstract":"The clonal origin of radiation-induced thymic lymphoma was studied in mice with cellular mosaicism for phosphoglycerate kinase (PGK). Repeated whole-body X-irradiations (4 doses, 1.7 Gy each) with intervals of 7 days resulted in development of thymic lymphomas in the mosaic mice. PGK from all lymphomas gave only a single spot on electrophoresis. The results demonstrate the single cell origin of the thymic lymphoma.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"127 1","pages":"1002-5"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75833127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 1984-11-01DOI: 10.20772/CANCERSCI1959.75.11_986
M. Tanaka, K. Kimura, S. Yoshida
Various human leukemoblastoid cell lines in logarithmic growth were incubated for 16 hr with 0.5 microM [3H]fluorodeoxyuridine (FdUrd) and the incorporation of [3H]FdUrd into DNA was measured. T-acute lymphoblastic leukemia (T-ALL) cell lines incorporated 2.4-7.0 times more [3H]FdUrd into newly synthesized DNA than cell lines from B-lymphoid, pre B-ALL, null cell ALL and acute myeloid leukemia cells. T-ALL cells incorporated 1.93-3.15 pmol of [3H]FdUrd nucleotides into DNA per 10(7) cells. The amount of [3H]FdUrd incorporated into DNA was inversely correlated with the activity level of uracil DNA glycosylase of the cells. On the other hand, no correlation was observed between the incorporation and the level of deoxyuridine triphosphate diphosphohydrolase of each cell line. However, the amount of FdUrd incorporated into DNA could not be correlated with the antiproliferative activity of FdUrd against these cell lines.
{"title":"Increased incorporation of 5-fluorodeoxyuridine into DNA of human T-lymphoblastic cell lines.","authors":"M. Tanaka, K. Kimura, S. Yoshida","doi":"10.20772/CANCERSCI1959.75.11_986","DOIUrl":"https://doi.org/10.20772/CANCERSCI1959.75.11_986","url":null,"abstract":"Various human leukemoblastoid cell lines in logarithmic growth were incubated for 16 hr with 0.5 microM [3H]fluorodeoxyuridine (FdUrd) and the incorporation of [3H]FdUrd into DNA was measured. T-acute lymphoblastic leukemia (T-ALL) cell lines incorporated 2.4-7.0 times more [3H]FdUrd into newly synthesized DNA than cell lines from B-lymphoid, pre B-ALL, null cell ALL and acute myeloid leukemia cells. T-ALL cells incorporated 1.93-3.15 pmol of [3H]FdUrd nucleotides into DNA per 10(7) cells. The amount of [3H]FdUrd incorporated into DNA was inversely correlated with the activity level of uracil DNA glycosylase of the cells. On the other hand, no correlation was observed between the incorporation and the level of deoxyuridine triphosphate diphosphohydrolase of each cell line. However, the amount of FdUrd incorporated into DNA could not be correlated with the antiproliferative activity of FdUrd against these cell lines.","PeriodicalId":74436,"journal":{"name":"Philosophia (Ramat-Gan, Israel)","volume":"203 1","pages":"986-92"},"PeriodicalIF":0.0,"publicationDate":"1984-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86837708","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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