Reproduction and breeding最新文献

{"title":"Exploring phenotypic variability in quantitative and qualitative traits in F2 population of local red rice","authors":"Masud Perves ,&nbsp;Abu Sayeed Md Hasibuzzaman ,&nbsp;Rashadul Hasan ,&nbsp;Md Mosanna Habib Emon ,&nbsp;Maria Chowdhory ,&nbsp;Md Shamiul Haque ,&nbsp;Arif Hasan ,&nbsp;Istiak Ahamad ,&nbsp;Sakina Khanam ,&nbsp;Shamsun Nahar Begum","doi":"10.1016/j.repbre.2025.10.001","DOIUrl":"10.1016/j.repbre.2025.10.001","url":null,"abstract":"<div><div>Red rice is nutrient-dense, yet local varieties display suboptimal ideotypes characterized by prolonged growth duration, susceptibility to lodging, and open panicles with reduced grain count, constraining its yield. We hybridized a well-adapted, high-yielding variety, Binadhan-17, with a red rice mutant (derived from local red rice) by cross and reciprocal cross, intending to uncover changes in quantitative and qualitative traits in the F₂ population. Histograms and descriptive analysis revealed the highest variation in key yield components, with the genotypic coefficient of variation (GCV) close to the phenotypic coefficient of variation (PCV), indicating genetic control over the environment. Variation in Genetic Advance (GA) and Genetic Advance as a Percent of the Mean (GAM) highlights the traits against which selection will be crucial. Days to maturity exhibited a significant negative correlation with grain-related and panicle traits in the cross-population, while plant height positively correlated with effective tillers, panicle length, and filled grains in both populations. The variation in the <em>Rc</em> gene, which governs color, was validated by the SSR marker RM180, effectively distinguishing the red pericarp group from the white group. This is the first analysis integrating <em>Rc</em> gene validation with reciprocal F₂ populations in red rice × high-yielding variety crosses, revealing substantial variation in plant architecture and panicle-related traits and offering significant scope for selection in subsequent breeding cycles.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 202-213"},"PeriodicalIF":0.0,"publicationDate":"2025-10-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145363566","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reproductive biology, hormonal induction efficiency, and larval rearing success of Amblypharyngodon mola (Hamilton, 1822)","authors":"Md Sharif Mia ,&nbsp;Shyamal Kumar Paul ,&nbsp;K.M. Mahfujur Rahman ,&nbsp;M. Maksud Alam ,&nbsp;Tania Perven ,&nbsp;Debasish Saha ,&nbsp;Bhakta Supratim Sarker ,&nbsp;Srijan Sarker","doi":"10.1016/j.repbre.2025.09.004","DOIUrl":"10.1016/j.repbre.2025.09.004","url":null,"abstract":"<div><div>The status of <em>Amblypharyngodon mola</em> in Bangladesh is categorized as least concern. This experiment aims to examine the breeding biology and produce fry through induced breeding of the species. To achieve, we measured the gonadosomatic index (GSI), fecundity, and sex ratio throughout the year. It was designed 12 treatments (T1-T12) with CPH, hCG, and ovaprim and one control (T0: without hormone dose) with four doses of each hormone and maintaining a fixed sex ratio of 1:1 (M:F). For the evaluation of induced breeding, measured latency time and hatching periods, fertilization, hatching, and survival rates and monitored the water temperature, pH, dissolved oxygen (DO), and total dissolved solids (TDS) of the supplied water. The survival rate of the larvae in cages was assessed after 30 days of rearing under four different conditions, i.e., the control group (without supplementary feed), LR1 (mustard oil cake), LR2 (egg custard), and LR3 (commercial feed powder). Additionally, water quality parameters were evaluated during this period. The highest GSI and absolute fecundity were recorded in mid-October (21.86 ± 2.87 % and 12.462 ± 1639 eggs/female, respectively), while the lowest (3.23 ± 0.41 % and 978 ± 283 eggs/female) were observed in late January. The sex ratio (F:M) ranged from 1.23:1 to 2.0:1 (average of 1.52:1). CPH had a shorter latency duration (6–7 h) than hCG (7–8 h) and ovaprim (8–9 h). Different treatments had 16–18 h hatching periods. The highest rates of fertilization, hatching, and survival were 75.83 ± 9.30, 76.06 ± 7.95 %, and 66.74 ± 4.12 %, respectively, with treatment T2 (CPH 6 mg/kg female and 3 mg/kg male). After 30 days, the highest survival rate (56 ± 6.32 %) was recorded in treatment LR2, followed by 48 ± 5.93 % (LR3), 41 ± 7.23 % (LR1), and 32 ± 6.87 % (control). Physicochemical parameters of water in incubator and cage were optimal. These findings show that fish farmers can benefit from bulk fry production for culture, protecting this species and promoting biodiversity.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 190-201"},"PeriodicalIF":0.0,"publicationDate":"2025-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145268893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chromosomal localization and variation analysis of 5S rDNA in autotriploid Carassius auratus","authors":"Kun Zhang ,&nbsp;Yuxin Zhang ,&nbsp;Xu Huang ,&nbsp;Chongqing Wang ,&nbsp;Xidan Xu ,&nbsp;Xiaowei Xu ,&nbsp;Li Yang ,&nbsp;Tingting Yu ,&nbsp;Xiang Luo ,&nbsp;Qingwen Xiao ,&nbsp;Yue Zhou ,&nbsp;Qinbo Qin","doi":"10.1016/j.repbre.2025.09.003","DOIUrl":"10.1016/j.repbre.2025.09.003","url":null,"abstract":"<div><div>Polyploidization can induce a flurry of genetic events and plays an important role in speciation. In this study, we comparatively analyzed the molecular organization, chromosomal locus in natural autotriploid (3nCC, 3<em>n</em> = 150) and artificial autotriploid gynogenetic (3nG, 3<em>n</em> = 150) <em>Carassius auratus</em>, diploid <em>Carassius auratus</em> (2nCC, 2<em>n</em> = 100) and <em>Carassius auratus</em> red var. (RCC, 2<em>n</em> = 100). Three distinct 5S rDNA sequence types were identified across 3nCC, 3nG 2nCC and RCC. Among these, the sequences in 3nG and 3nCC matched perfectly with those in RCC and 2nCC, respectively. The NTS regions of 5S rDNA in 3nG and 3nCC showed significant base variations compared to RCC and 2nCC, separately. FISH with 5S rDNA probe indicated that the expected number of maternal chromosomal loci was found in both 3nCC and 3nG. These observations indicated that they provide theoretical support for the study of the evolutionary modes and characteristics of rDNA in vertebrates.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 180-189"},"PeriodicalIF":0.0,"publicationDate":"2025-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive screening and evaluation of diverse Mediterranean barley genotypes for salt tolerance using multiple agro-morphological and tolerance indices criteria","authors":"Said Bouhraoua ,&nbsp;Mohamed Ferioun ,&nbsp;Abdelali Boussakouran ,&nbsp;Douae Belahcen ,&nbsp;Khalil Hammani ,&nbsp;Said Louahlia","doi":"10.1016/j.repbre.2025.09.002","DOIUrl":"10.1016/j.repbre.2025.09.002","url":null,"abstract":"<div><div>Salinity tolerance in barley is essential for food security and as a model for crop resilience to climate change. Twenty-three barley genotypes were evaluated under non-saline (0 dS/m) and saline (12 dS/m) irrigation regimes. To evaluate the salinity resilience of various barley genotypes, grain yield was assessed under both salinity treatment. Eight tolerance indices were calculated to comprehensively characterize performance: Salinity Susceptibility Index (SSI), Tolerance Index (TOL), Stress Tolerance Index (STI), Harmonic Mean Productivity (HMP), Geometric Mean (GM), Geometric Mean Productivity (GMP), Yield Stability Index (YSI), and Mean Productivity (MP). Analysis of variance showed genotype variation and showed that salinity had a considerable impact on agro-morphological parameters. Grain yield shown significant correlations with GMP, STI, MP, and HMP in non-stress situations, indicating that these measurements correctly estimate high performance. Principal component analysis (PCA) and heatmap visualization identified four genotypes ‘Assiya’, ‘Laanaceur’, ‘Tombari’, and ‘Giza130’ as top performers under non-saline irrigation, displaying elevated values across these indices. Under saline stress (Ys), grain yield correlated positively with Yield Index (YI), YSI, and GM, highlighting their relevance for stress resilience. Three genotypes ‘Massine’, ‘Tozeur’, and ‘Giza125’ exhibited the highest yields and index scores under salinity, suggesting superior tolerance. In contrast, ‘Manal’, ‘Amira’, ‘Elarich’, ‘Rihane’, and ‘Temellalet’ were more sensitive to salt stress. Overall, STI, MP, HMP, and GMP demonstrated consistent, significant positive associations with grain yield across both irrigation treatments, underscoring their utility as robust selection criteria. These findings support using these indices in breeding programs targeting Mediterranean barley genotypes with enhanced salinity tolerance.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 171-179"},"PeriodicalIF":0.0,"publicationDate":"2025-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145159105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Generation of fast-growth grass carp by mutation of mstnb via CRISPR/Cas9 system","authors":"Pengfei Zhao ,&nbsp;Yuan Shen ,&nbsp;Jiaxiang Cheng ,&nbsp;Liang Zhang ,&nbsp;Zeting Qu ,&nbsp;Wenbo Li ,&nbsp;Xingyong Liu ,&nbsp;Minghui Li ,&nbsp;Shengfei Dai","doi":"10.1016/j.repbre.2025.09.001","DOIUrl":"10.1016/j.repbre.2025.09.001","url":null,"abstract":"<div><div>Myostatin (<em>mstn</em>) is a highly conserved negative regulator of myogenesis and myofiber growth in vertebrates. In recent years, the CRISPR/Cas9 (Clustered Regularly Interspaced Short Palindromic Repeats) system has been widely used for genetic breeding in aquaculture. Targeted disruption of <em>mstn</em> in various teleost species has led to enhanced growth traits. In this study, two <em>mstn</em> paralogs, designated <em>mstna</em> and <em>mstnb</em>, were identified in grass carp (<em>Ctenopharyngodon idella</em>). Tissue distribution analysis using RT-PCR revealed that <em>mstnb</em>, but not <em>mstna</em>, was expressed in muscle tissue, suggesting a potential role for the <em>mstnb</em> gene in grass carp muscle growth. To investigate the role of <em>mstnb</em> in skeletal muscle growth, we adopted CRISPR/Cas9 to disrupt the <em>mstnb</em> gene by co-injecting Cas9 protein and sgRNA into one-cell stage embryos. Approximately 85 % of injected F0 individuals carried mutations at the <em>mstnb</em> target site, including various frameshift indel mutations. Compared to their wild-type counterparts, the <em>mstnb</em> F0 mutants showed significantly increased body weight and length. Quantitative real-time PCR analysis confirmed a significant reduction in <em>mstnb</em> expression in the F0 mutants. Additionally, two growth-related genes, <em>myod1</em> and <em>vegfba</em>, exhibited significant upregulation in mutant muscle tissue. Histological examination revealed no significant difference in the thickness of individual muscle fibers between WT siblings and the F0 mutants. However, a significant increase in the total number of muscle fibers was observed in the <em>mstnb</em> F0 mutants. We successfully generated <em>mstnb</em> mutants with enhanced muscle growth, which may promote grass carp production in aquaculture practice. Overall, our study provided a valuable resource for the genetic breeding of grass carp.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 4","pages":"Pages 163-170"},"PeriodicalIF":0.0,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145107931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"From bench to bedside: Mouse models in translating infertility research to clinical applications in assisted reproduction","authors":"Pallav Sengupta ,&nbsp;Sulagna Dutta","doi":"10.1016/j.repbre.2025.08.003","DOIUrl":"10.1016/j.repbre.2025.08.003","url":null,"abstract":"<div><div>The use of animal models, principally mice, has been instrumental in advancing our understanding of reproductive biology. Mice serve as a powerful tool due to their genetic and physiological similarities to humans, short reproductive cycles, and ease of genetic manipulation. This review highlights the pivotal role of mouse models in reproductive research, emphasizing the genetic approaches used to elucidate gene functions. Reverse genetics, involving gene expression modifications followed by phenotypic analysis, and forward genetics, which starts with phenotypic observations to identify genetic causes, are key methodologies discussed. Mouse models have provided significant insights into various aspects of reproduction, including spermatogenesis, oogenesis, hormonal regulation, and the impact of specific genes on fertility. Notable examples include the use of knockout and transgenic mice to study gene functions related to fertility and the development of models for studying human reproductive disorders such as polycystic ovarian syndrome and endometriosis. Additionally, the review addresses the importance of mouse models in assisted reproductive technologies (ART), exploring their contributions to understanding ART-induced epigenetic changes and long-term outcomes. This comprehensive examination underscores the indispensable role of mice in reproductive biology research, paving the way for translating these findings into clinical applications for human infertility.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 153-162"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144932252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Captive breeding practices and insights on four indigenous Betta species of Sarawak","authors":"Ahmad Syafiq Ahmad Nasir ,&nbsp;Mohd Armeen Zulkanaini ,&nbsp;Jeffery Mahmud ,&nbsp;Ruhana Hassan ,&nbsp;Mohamad Faizul Mat Isa ,&nbsp;Samsur Mohamad","doi":"10.1016/j.repbre.2025.08.002","DOIUrl":"10.1016/j.repbre.2025.08.002","url":null,"abstract":"<div><div>Sarawak, located on the island of Borneo, is home to several indigenous <em>Betta</em> species, many of which face threats from habitat degradation. Despite their ecological and ornamental significance, limited information exists on their captive breeding potential. This study investigated the reproductive behavior and larval development of four native <em>Betta</em> species (<em>Betta brownorum</em>, <em>Betta ibanorum</em>, <em>Betta macrostoma</em>, and <em>Betta taeniata</em>) under controlled conditions. A total of 46 wild specimens were collected from four locations across Sarawak and subjected to species-specific breeding trials in aquaria. <em>B. brownorum</em>, a bubble-nester, exhibited nest-building and egg deposition behavior, with hatching observed within 3–4 days. In contrast, the three mouthbrooding species exhibited paternal incubation, which lasted 3–4 weeks. Larval development was monitored over time, showing steady increases in total length and distinct developmental stages were documented. These findings confirm the feasibility of captive breeding in all four species and highlight differences in reproductive strategy and larval growth patterns. The outcomes provide valuable baseline data for conservation planning and the development of ex-situ breeding programs for Sarawak's threatened <em>Betta</em> species.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 144-152"},"PeriodicalIF":0.0,"publicationDate":"2025-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of interspecific crossing line x tester (C. annuum and C. frutescens) on chili fruit based on image processing and multivariate analysis","authors":"Muhammad Fuad Anshori ,&nbsp;Abd Haris Bahrun ,&nbsp;Nuniek Widiayani ,&nbsp;Dwi Lestari ,&nbsp;Reynaldi Laurenze ,&nbsp;Muhammad Faried ,&nbsp;Nirwansyah Amier ,&nbsp;Nurafika Nurafika","doi":"10.1016/j.repbre.2025.08.001","DOIUrl":"10.1016/j.repbre.2025.08.001","url":null,"abstract":"<div><div>Developing interspecific crosses of chili peppers is one way to expand the range expected by the market. Common interspecific crosses were <em>Capsicum annum</em> and <em>Capsicum frutescens</em>. However, systematic and detailed evaluations of the effects of these crosses on fruit quality have rarely been conducted. Therefore, the objectives of this study were (1) to determine effective evaluation criteria for chili fruit from interspecific crosses of <em>C. annuum</em> and <em>C. frutescens,</em> and (2) to identify the effect of interspecific crosses on for offspring based on evaluation criteria. This study was conducted in two stages. The first stage was a reciprocal line x tester cross between <em>C. frutescens</em> (Sonar, Bara, Bhaskara) and <em>C. annuum</em> (Pilar and Igo). The second stage involved evaluation of the line × tester hybrids. The evaluation was conducted in a randomized complete block design with genotypes, 12 interspecies hybrids, and five elders as the main factors, repeated three times. Observations focused on five agronomic characters, and 14 image-processing characters were systematically analyzed using multivariate analysis. Based on the results of this study, the characteristics of HUE (H² = 68.18 %), fruit area (H² = 84.85 %), and fruit weight (H² = 50.00 %) can be used as criteria for assessing the crossing potential between the two species. These characters demonstrate that <em>C. frutescens</em> influences the fruit agronomic potential of the hybrid progeny compared to <em>C. annuum,</em> both as a line and tester. Even so, HUE can distinguish between the influence of the two species when acting as females and males. Therefore, the HUE is recommended as an adequate colorimetric criterion for assessing the interaction potential of the hybrid progeny of the two species.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 135-143"},"PeriodicalIF":0.0,"publicationDate":"2025-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144908196","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transcriptome-based analysis reveals the role of Hedgehog signaling in regulating slow muscle autophagy in Chinese perch","authors":"Yangyang Meng,&nbsp;Wei Zeng,&nbsp;Xin Liao,&nbsp;Lingtao Nie,&nbsp;Junzhao Wang,&nbsp;Xin Zhu,&nbsp;Jianshe Zhang,&nbsp;Wuying Chu","doi":"10.1016/j.repbre.2025.07.001","DOIUrl":"10.1016/j.repbre.2025.07.001","url":null,"abstract":"<div><div>As one of the structural tissues of fish skeletal muscles, slow muscle plays an important role in physiological and metabolic activities. The Hedgehog (Hh) signaling pathway has been reported to be involved in regulating the development and metabolism of slow muscle in fish. The underlying molecular mechanisms by which the Hedgehog (Hh) signaling pathway governs metabolic homeostasis within the slow muscle tissue of fish remain an enigma. In order to reveal the functional regulatory role of the Hh signaling pathway in the slow muscle of Chinese perch (<em>Siniperca chuatsi</em>), Cyclopamine was used to inhibit the Hh signaling pathway and Illumina sequencing technology was employed to explore the effect of inhibiting the Hh signaling pathway on slow muscle transcriptomes. In this study, a total of 666 differentially expressed genes (DEGs) were obtained between the control and Cyclopamine-treated groups. Functional enrichment analyses showed that the DEGs are mainly enriched in signaling pathways related to autophagy and metabolism, such as the mTOR signaling pathway, the autophagy pathway and the FoxO signaling pathway. The Western blotting analysis showed that the autophagy levels increased after treatment with Cyclopamine. Transmission electron microscopy results showed that the number of autophagic lysosomes was reduced in the Cyclopamine-treated group. The above results suggest that the Hh signaling pathway may play a key role in the regulation of autophagy and metabolism in slow muscle of Chinese perch, thus ensuring normal physiological activities in their muscles. In addition, the present study provides a direction for further elucidating the role of Hh signaling pathway in regulating the dynamic balance of metabolism in the slow muscle of Chinese perch.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 125-134"},"PeriodicalIF":0.0,"publicationDate":"2025-07-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Levels of serum and seminal fluid RANKL and osteoprotegerin in differentiating oligozoospermic and idiopathic infertile males: a case-control study","authors":"Osamah A. Layih,&nbsp;Basil O. Saleh","doi":"10.1016/j.repbre.2025.05.002","DOIUrl":"10.1016/j.repbre.2025.05.002","url":null,"abstract":"<div><div>Infertility is defined as the inability to conceive after one year of unprotected intercourse, with male factors accounting to about 50 % of cases. The study aimed to investigate the potential role of receptor activator of nuclear factor kappa-B ligand (RANKL) and osteoprotegerin (OPG) in differentiating between oligozoospemia and idiopathic subtypes of male infertility. A case-control study was conducted at the infertility center of Al-Batool Teaching Hospital in Diyala Governorate, Iraq, by the Department of Biochemistry, College of Medicine, University of Baghdad between April 2024 and January 2025. The study included 82 males, aged 20–55 years, divided into three groups based on seminal fluid analysis: 30 normozoospermic males (control group), 25 oligozoospermia patients, and 27 patients with idiopathic infertility. Serum and seminal fluid levels of (RANKL) and (OPG) were measured using enzyme-linked immunosorbent assays. Serum RANKL levels were significantly elevated in oligozoospermic patients (297.93 <em>±</em> 85.99 pg/mL) compared to idiopathic infertility (147.16 <em>±</em> 49.58 pg/mL) and normozoospermic controls (116.29 <em>±</em> 20.95 pg/mL; p &lt; 0.001). A similar pattern was observed in seminal RANKL levels, with oligozoospermia patients showing higher levels (402.39 <em>±</em> 132.79 pg/mL) than controls (175.84 <em>±</em> 24.46 pg/mL) (p &lt; 0.001). In contrast, serum (OPG) levels were highest in normozoospermic controls (3.45 <em>±</em> 0.98 ng/mL) and lowest in oligozoospermic patients (1.29 <em>±</em> 0.56 ng/mL; p &lt; 0.001). Seminal OPG exhibited the most pronounced differences, with controls (at 2.88 <em>±</em> 0.89 ng/mL) compared to (1.00 <em>±</em> 0.45 ng/mL) in oligozoospermic patients (p &lt; 0.001). Reservoir operating characteristics (ROC) analysis demonstrated exceptional diagnostic utility for serum RANKL Area under the curve (AUC: 0.999) and OPG (AUC: 0.980) in differentiating oligozoospermia from normozoospermia. Serum RANKL showed 100 % sensitivity and 96.67 % specificity at a threshold &gt;148.1 pg/mL, while OPG showed 100 % sensitivity and 92 % specificity at <em>≤</em>2.2 ng/mL. For distinguishing idiopathic cases from normozoospermia, serum RANKL and OPG showed moderate diagnostic value (AUC 0.683 and 0.637). Seminal fluid measurements of both markers demonstrated moderate to excellent diagnostic performance for both comparisons. In conclusion, serum RANKL and OPG levels may serve as valuable biomarkers for differentiate between subtypes of male infertility.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 119-124"},"PeriodicalIF":0.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144623799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}